Thermo Scientific Nanodrop Onec User Manual

NanoDrop Micro-UV/Vis Spectrophotometer

NanoDrop One

with

NanoDrop QC Software

User Guide

269-342200 NanoDrop QC UG Revision A December 2019

DYMO and LabelWriter are either trademarks or registered trademarks of Newell Rubbermaid in the United States and/or other countries. Wi-Fi is either a trademark or a registered trademark of Wi-Fi Alliance in the United States and/or other countries. Bluetooth is either a trademark or a registered trademark of Bluetooth Special Interest Group. Windows is either a trademark or a registered trademark of Microsoft Corporation in the United States and/or other countries. All other trademarks are the property of Thermo Fisher Scientific inc. and its subsidiaries.

For U.S. Technical Support, please contact:

Thermo Fisher Scientific 3411 Silverside Road Tatnall Building, Suite 100 Wilmington, DE 19810 U.S.A.

Telephone: 302 479 7707

For International Support, please contact:

http://www.nanodrop.com/support

Contact your local distributor. For contact information go to:

http://www.nanodrop.com/Order.aspx

Toll Free: 1 877 724 7690 (U.S. & Canada only) E-mail: nanodrop@thermofisher.com

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or errorfree and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.

For Research Use Only. This instrument or accessory is not a medical device and is not intended to be used for the prevention, diagnosis, treatment or cure of disease.

WARNING Avoid an explosion or fire hazard. This instrument or accessory is not designed for use in an explosive atmosphere.

Chapter 1 About the Spectrophotometer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Cuvette Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

USB-A port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

PR-1 Pedestal Reconditioning Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4

PV-1 Performance Verification Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Instrument Detection Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Chapter 2 Instrument Set up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Update Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Setting Up User Account Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

User Account Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Security Administration Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Chapter 3 Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

To make UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Best practices for UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . .13

UV-Vis Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Settings for UV-Vis Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Measure Chemometrics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Measure Chemometrics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Create Chemometrics Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Edit Chemometrics Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Chemometrics Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Settings for Chemometric Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Thermo Scientific NanoDrop OneC with NanoDropQC Software User Guide iii

Contents

Measure Custom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29

Measure using a Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Delete Custom Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Custom Method Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Manage Custom Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

Chapter 4 Learning Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

Micro-Volume Sampling—How it Works. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Set Up the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Measure a Micro-Volume Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Measure a Sample Using a Cuvette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Prepare Samples and Blanks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Basic Instrument Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

NanoDrop One Home Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

NanoDrop One Measurement Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74

NanoDrop One General Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90

Instrument Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .100

Data Deletion Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

PC Control Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

PC Control Home Screen overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

Control options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108

History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108

Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109

Chapter 5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111

Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

Cleaning the Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

Maintaining the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

Cleaning the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114

Reconditioning the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

Decontaminating the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119

Maintaining the Cuvette Sampling System . . . . . . . . . . . . . . . . . . . . . . . . . . . 121

Instrument Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122

Intensity Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122

Performance Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

Pedestal Image Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130

Chapter 6 Safety and Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131

Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132

Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133

iv NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

About the Spectrophotometer

NanoDrop OneC Spectrophotometer

Arm

Pedestal

Cuvette holder

NOTICE Locate the instrument away from air vents and exhaust fans to minimize

evaporation

The Thermo Scientific™ NanoDrop™ OneC is a compact, stand-alone UV-Visible spectrophotometer developed for micro-volume analysis of a wide variety of analytes. The patented sample retention system enables the measurement of highly concentrated samples without the need for dilutions.

The NanoDrop One NanoDrop QC PC Control software can be installed on a local PC and used to control the instrument and view data. The instrument can be connected to an optional printer with a USB cable or to a remote printer through an Ethernet connection or wireless network.

NOTICE Before operating a NanoDrop One instrument, please read the safety and

operating precautions and then follow their recommendations when using the instrument.

system comes with preloaded software and a touchscreen display.

Thermo Scientific NanoDrop OneC with NanoDropQC Software User Guide 1

About the Spectrophotometer

Features

Touchscreen

TheNanoDrop OneC spectrophotometer features the patented micro-volume sample

retention system. The NanoDrop One

also features a cuvette holder for analyzing dilute

samples using standard UV-visible cuvettes.

TheNanoDrop OneC comes with a built-in, 7-inch high-resolution touchscreen preloaded with easy-to-use instrument control software. The touchscreen can slide left or right to accommodate personal preference, and tilt forward or back for optimal viewing

2 NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

Cuvette Holder

Instrument light path

About the Spectrophotometer

Cuvette holder

Features

USB-A port

The NanoDrop OneC includes a cuvette holder for measuring dilute samples, colorimetric assays, cell cultures and kinetic studies. The cuvette system has these features:

• extended lower detection limits

• 37 °C heater option for temperature-sensitive samples and analyses

• micro-stirring option to ensure sample homogeneity and support kinetic studies

For details, see Measure a Sample using a Cuvette.

Two more USB-A ports are located on instrument back panel

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 3

About the Spectrophotometer

Accessories

This section lists the accessories included for use with the NanoDrop OneC.

PR-1 Pedestal Reconditioning Kit

Specially formulated conditioning compound that can be applied to the pedestals to restore them to a hydrophobic state (required to achieve adequate surface tension for accurate sample measurements). The kit includes conditioning compound and applicators. For more information, see

Reconditioning the Pedestals.

PV-1 Performance Verification Solution

Liquid photometric standard used to check instrument performance. For more information, see Performance Verification.

4 NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

Instrument Detection Limits

Measurement Location Pathlength (mm)

Pedestal 1.0 12.5

About the Spectrophotometer

Instrument Detection Limits

Upper Detection Limit (10 mm Equivalent Absorbance)

0.2 62.5

0.1 150

0.05 300

0.03 550

Cuvette 10 1.5

2 7.5

115

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 5

About the Spectrophotometer

This page is intentionally blank.

6 NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

Instrument Set up

Register Your Instrument

To register your instrument

1. Do one of the following:

On the website, locate NanoDrop One Registration and follow the instructions to register the instrument.

Update Software

Quickly and easily download and install the latest NanoDrop One software and release notes from our website. Follow the steps to update or upgrade the software on your local instrument and/or install or update the NanoDrop QC software on a personal computer (PC). An Internet connection is required to download software.

To install or update NanoDrop QC software on a PC

1. Insert the USB flash drive containing the installer software into an available USB port on

instrument. An Internet connection is required for registration.

– From any PC that is connected to the Internet, use any web browser to navigate to

our website.

your PC

2. Launch Start.exe. The software installer will run.

To install or update NanoDrop QC software on the instrument

1. Copy the .zip file with the new software from your computer to a USB storage device. Do not attempt to unzip the folder.

2. Insert the USB device into any USB port on the NanoDrop One

3. From the instrument Home screen, tap Settings > System > Update Software and choose the latest version of software.

Thermo Scientific NanoDrop OneC with NanoDropQC Software User Guide 7

instrument.

Instrument Set up

Setting Up User Account Control

User account control is managed using the Security Administration application. When you launch Security Administration, you will need to enter your Windows log-in information.

User Account Control

Launch the Security Administration application and select NanoDrop QC from the directory on the left to reveal Access Control and System Policies.

Access control

Access control is used to grant or deny individual users or groups access to protected features in the instrument application. Add and remove users and groups to the access list and set access rights using the drop-down for each entity.

System policies

8 NanoDrop One

System Policies is used to set options that define the behavior of the client application. See

“Security Administration Policies.”

with NanoDropQC Software User Guide Thermo Scientific

Security Administration Policies

System policies allow you to assign data and method creation and deletion and editing privileges for users and groups.

Launch the Security Administration application and select NanoDrop QC -> System

Policies

You can add, delete, or edit policy groups and enable or disable the group’s users permission to delete data. When you are finished, select Save. Changes will take effect the next time NanoDrop QC is launched. Changes made to the Security Administration policies are applicable to the local PC only and will not affect other computers on the network.

Instrument Set up

Setting Up User Account Control

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 9

Instrument Set up

Technical Support

For U.S./Canada Support, please contact:

Thermo Fisher Scientific 3411 Silverside Road Tatnall Building, Suite 100 Wilmington, DE 19810 U.S.A.

Telephone: 302 479 7707 Toll Free: 1 877 724 7690 (U.S. & Canada only) Fax: 302 792 7155 E-mail: nanodrop@thermofisher.com Website: www.thermoscientific.com/nanodrop

For International Support, please contact:

Contact your local distributor. For contact information go to:

http://www.nanodrop.com/Order.aspx

If you are experiencing an issue with your system, refer to the troubleshooting information. If the issue persists, contact us. If you are outside the U.S.A. and Canada, please contact your local distributor.

If your instrument requires maintenance or repair, contact us or your local distributor.

10 NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

Applications

Use the NanoDrop OneC to perform UV-Vis, Chemometrics, or your own custom measurements.

The UV-Vis application can be set up directly from the touchscreen and allows the instrument to function as a conventional spectrophotometer. Up to 40 wavelengths from 190 nm to 850 nm can be monitored and reported.

The Chemometrics application allows you to use your unique chemometrics method. The method is created using the NanoDrop QC PC Control software that is installed on your PC and then loaded into the instrument though a USB storage device. See the NanoDrop QC PC Control software to learn about Chemometric method features that are supported.

The Custom application provides additional flexibility for the method that you use with the instrument. See the NanoDrop QC PC Control software to learn about custom method features that are supported.

• Measure UV-Vis 12

• Measure Chemometrics 18

• Measure Custom 29

Thermo Scientific NanoDrop OneC with NanoDropQC Software User Guide 11

Applications

Measure UV-Vis

The UV-Vis application allows the instrument to function as a conventional spectrophotometer. Sample absorbance is displayed on the screen from 190 nm to 850 nm. Up to 40 wavelengths can be designated for absorbance monitoring and inclusion in the report. Automatic pathlength adjustment and a single-point baseline correction can also be used.

Measures the absorbance of any sample at up to 40 wavelengths across the ultra-violet (UV) and visible regions of the spectrum.

Measure UV-Vis

Reported Results

Settings

Detection Limits

To make UV-Vis measurements

NOTICE

• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may cause permanent damage.

• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently damage the quartz fiber optic cables.

Before you begin...

Before taking pedestal measurements with the NanoDrop One instrument, lift the instrument arm and clean the upper and lower pedestals. At a minimum, wipe the pedestals with a new laboratory wipe. For more information, see Cleaning the Pedestals.

To measure a sample using the UV-Vis application

1. From the Home screen, select UV-Vis.

2. Specify up to 40 wavelengths to monitor (or you can specify them later if desired) and whether automated pathlength adjustment, analysis wavelength, and baseline correction will be used.

12 NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

Applications

Measure UV-Vis

3. Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm, or insert the blanking cuvette into the cuvette holder.

Tip: If using a cuvette, make sure to align the cuvette light path with the instrument light path.

4. Tap Blank and wait for the measurement to complete.

Tip: If Auto-Blank is On, the blank measurement starts automatically after you lower the arm. (This option is not available for cuvette measurements.)

5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove the blanking cuvette.

6. Pipette 1-2 µL sample solution onto the pedestal and lower the arm, or insert the sample cuvette into the cuvette holder.

7. Start the sample measurement:

– Pedestal: If Auto-Measure is On, lower arm; if Auto-Measure is off, lower arm and

tap Measure.

– Cuvette: Tap Measure.

When the sample measurement is completed, the spectrum and reported values are displayed (see the next section).

8. When you are finished measuring samples, tap End Experiment.

9. Lift the arm and clean both pedestals with a new wipe, or remove the sample cuvette.

Best practices for UV-Vis measurements

• Ensure the sample absorbance is within the instrument’s absorbance detection limits.

• Blank with the same buffer solution used to re-suspend the analyte of interest. The

blanking solution should be a similar pH and ionic strength as the analyte solution.

• Run a blanking cycle to assess the absorbance contribution of your buffer solution. If the

buffer exhibits strong absorbance at or near an analysis wavelength, you may need to choose a different buffer or application. See Choosing and Measuring a Blank for more information.

• For micro-volume measurements:

– Ensure pedestal surfaces are properly cleaned and conditioned.

– Ensure samples are homogeneous before taking a measurement. Avoid introducing

bubbles when mixing and pipetting.

– Follow best practices for micro-volume measurements.

– Use a 1-2 µL sample volume. See Recommended Sample Volumes for more

information.

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 13

Applications

Measure UV-Vis

• For cuvette measurements (NanoDrop OneC instruments only), use compatible cuvettes

and follow best practices for cuvette measurements.

UV-Vis Reported Results

UV-Vis measurement screen

For each measured sample, this application shows the absorbance spectrum and a summary of the results. Here is an example as it appears on the local instrument display:

Menu of options;

tap to open

UV-Vis spectrum

Sample name; tap to edit

Absorbance at userdefined wavelength 1 (450 nm)

Tap to edit

Absorbance at user- defined wavelength 2 (623 nm)

Tap to edit

Tap to add

Tap row to

select sample and update spectrum; tap more rows to overlay up to five spectra. Press and hold sample row to view

Pinch and zoom to adjust axes; double-tap to reset

14 NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

Swipe screen left to view table with more measurement results

Tap to end experiment and export data

measurement details.

Drag tab down/up to see

more/less sample data

Applications

Measure UV-Vis

Note Micro-volume absorbance measurements and measurements taken with nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.

UV-Vis reported values

The initial screen that appears after each measurement (see previous image) shows a summary of the reported values. To view all reported values, press and hold the sample row. Here is an example:

Application

User-defined wavelengths

Sampling method

Baseline correction wavelength

Sample name;

tap to edit

Absorbance at 635 nm

Date/time measured

Automated pathlength setting

Baseline correction absorbance

Absorbance at 450 nm

Absorbance at 623 nm

Note Scroll up to display absorbance values for any additional user-defined wavelengths.

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 15

Applications

Measure UV-Vis

Below is an example of the measurement screen with reported values as it appears in the NanoDrop QC PC software:

Back to Home screen (ends experiment)

Sample name; select to edit

Run blank

Measure sample solution

Select to toggle Auto-Measure ON/OFF

Tips: Auto-Measure default is ON Auto-Blank default is OFF

Select to add absorbance at userdefined wavelengths

With sample selected, click and drag an area to zoom Right-click and select Autoscale to fit spectra to window

Tips:

Click sample row to select sample and update spectrum Shift-click multiple sample rows to overlay up to five spectra Click a sample and hover locations on spectra to view measurement values

End experiment and export data

Sample rows

16 NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

Settings for UV-Vis Measurements

To show the UV-Vis settings, from the Home screen, select UV-Vis.

Setting Available Options Description

Applications

Measure UV-Vis

Monitored wavelengths

Enter up to 40 wavelengths between 190 nm and 850 nm

Analytical Wavelength Any wavelength between

190 nm and 850 nm

Automated Pathlength On or Off

(affects pedestal measurements only)

User-defined wavelengths to be measured and reported at run time. Absorbance values for the first three entered wavelengths

are displayed in the measurement screen. To see absorbance values for 8 monitored wavelengths, swipe left in the measurement screen to show the Data table. To see all monitored wavelengths, press and hold a sample row to show the Sample

Details screen (scroll up to display absorbance values for any

additional user-defined wavelengths).

Note: If Baseline Correction is selected, all displayed absorbance values are the corrected values.

This is the wavelength the software will use to determine the pathlength selection.

Optional automated pathlength selection. Allows the software to use the optimal (shorter) pedestal pathlength for high concentration samples to help prevent detector saturation (see

Detection Limits for details).

• When selected, the shorter pathlength is used when any wavelength between 220 nm and 850 nm has 10 mm equivalent absorbance value of 12.5 or higher. For wavelengths between 190 nm and 219 nm the change to the shorter pathlength occurs when any wavelength in this range has a 10 mm equivalent absorbance value of 10 or higher.

• When deselected, the pedestal pathlength is restricted to 10 mm across all wavelengths.

Note: In either case, displayed absorbance values have been normalized to a 10 mm pathlength equivalent.

Baseline Correction On or off

Optional user-defined baseline correction. Can be used to

correct for any offset caused by light scattering particulates by Enter baseline correction wavelength in nm or use default value (750 nm)

subtracting measured absorbance at specified baseline correction

wavelength from absorbance values at all wavelengths in sample

spectrum. As a result, absorbance of sample spectrum is zero at

specified baseline correction wavelength.

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 17

Applications

Measure Chemometrics

Make chemometric measurements.

Measure Chemometrics

Create Chemometric Method

Edit Chemometric Method

Reported Results

Settings

Detection Limits

Measure Chemometrics

The NanoDrop OneC model instrument can be used to make chemometric measurements.

Note The instrument arm can be up during cuvette measurements, which allows you to add reagents to the sample solution if desired.

To make chemometric measurements

NOTICE

• To prevent damage from spills, keep containers of liquids away from the instrument.

• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument ans may cause permanent damage.

Chemometric methods can only be created on a personal computer running the NanoDrop QC PC Control software. If you want to run a chemometric method and store the measurement results on the instrument, the method must also reside on the instrument. (This is the only way to run a chemometric method if your instrument is not connected to the computer with an Ethernet cable.)

18 NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

Applications

Measure Chemometrics

Load a chemometrics method

To measure a sample using a chemometrics method, you must first load the method onto the instrument.

To load chemometrics methods onto the instrument:

1. Export the method from the personal computer and copy the method file to the root of a portable USB device such as a memory stick.

Method files have a “.qmethod” filename extension.

2. Connect the USB device to one of the USB ports on the instrument.

3. From the Home screen, select the Chemometric Application icon

4. Use the list box at the top of the screen to indicate the USB port used.

5. Select Load Method.

A message box shows the NanoDrop One methods available on the selected USB device.

6. Select one or more method names in the Load Method box to select the methods to load.

7. Select Load.

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 19

Applications

Measure Chemometrics

To measure a sample using the chemometrics application

1. From the Home screen, select the Chemometrics icon.

The Chemometrics Method Selection screen is displayed. If one or more chemometric methods exist in the currently selected Data Storage Location, they will be listed in the Select Method box. A description of the selected method appears in the Method Details box.

2. Select a method:

20 NanoDrop One

• select an existing method by selecting the method name in the Select Method box.

3. Select Run Method.

4. Follow the on-screen instructions to measure a sample.

with NanoDropQC Software User Guide Thermo Scientific

Applications

Measure Chemometrics

To measure a sample using the chemometrics application from the PC software

1. From the Home screen, select the Chemometric Method icon.

The Chemometrics Method Selection screen is displayed. If one or more chemometric methods exist in the currently selected Data Storage Location, they will be listed in the method selection pane below the search feature. Details of the selected method appears in the method details pane to the right.

2. Select a method:

• select an existing method by selecting the method name in the method selection pane.

3. Select .

Follow the on-screen instructions to measure a sample.

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 21

Applications

Measure Chemometrics

Create Chemometrics Method

Chemometrics methods can be created only on the NanoDrop QC PC Control software. However, once the method is created, it can be saved in the NanoDrop One database on the local instrument, or in the NanoDrop QC database on the PC. To create a new Chemometrics method:

– From the NanoDrop QC software, select the Chemometric Method icon

– From the Chemometric Method Management pane, select

22 NanoDrop One

– Enter both a name and description for your chemometric method

– Select the quant method (.qnt fiile) you want to use

with NanoDropQC Software User Guide Thermo Scientific

– Adjust the method settings as desired

Applications

Measure Chemometrics

– Select Save

Note The method is saved in the currently selected Data Storage Location (local instrument or a connected PC).

– To run the method, select Run Method

Edit Chemometrics Method

Chemometrics methods can be edited only on the NanoDrop QC software. To edit an existing chemometrics method:

– From the NanoDrop QC software, select the Chemometric Method icon

– From the Chemometric Method Management screen, select the method you would

like to edit from the list of loaded methods.

– From the drop-down menu select Edit.

– Adjust the method settings as desired. You can select the components to be displayed

on the LC.

– Select Save

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 23

Applications

Measure Chemometrics

Chemometrics Reported Results

Chemometrics method measurement screen (shown from Data Viewer)

For each measured sample, this application shows the absorbance spectrum and a summary of the results. Here is an example:

Menu of options;

tap to open

UV-visible spectrum

Analyte concentrations

Tap row to select sample and update spectrum; tap more rows to overlay up to five spectra.

Press and hold sample

row to view measurement details.

Drag tab down/up to

see more/less

Pinch and zoom to adjust axes; double-tap to reset

24 NanoDrop One

with NanoDropQC Software User Guide Thermo Scientific

sample data

Swipe screen left to view table with more measurement results

Note Micro-volume absorbance measurements and measurements taken with nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.

Applications

Measure Chemometrics

Chemometrics method reported values

The initial screen that appears after each measurement (see previous image) shows a summary of the reported values. To view all reported values, press and hold the sample row. Here is an example:

Method name

Sampling method

Sample name;

tap to edit

Date/time measured

Analyte concentration

Method details

Thermo Scientific NanoDrop One

with NanoDropQC Software User Guide 25

Applications

Measure Chemometrics

Data Table

To see the data table, swipe the rate measurement screen (see above) to the left. Each row in the table shows the absorbance values at all user-defined wavelengths at a given stage and time. Scroll down to see measurement information that is out of view. The image below highlights the available features.

Measurement number

Sample name

Analyte concentration

Press and hold row to view measurement details

26 NanoDrop One

Swipe screen right to return

to Rate measurement screen

with NanoDropQC Software User Guide Thermo Scientific

Tap to end experiment and export data

+ 114 hidden pages