Thermo Scientific Nanodrop Onec User Manual

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NanoDrop Micro-UV/Vis Spectrophotometer

NanoDrop One

with

NanoDrop QC Software

User Guide

269-342200 NanoDrop QC UG Revision A December 2019

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DYMO and LabelWriter are either trademarks or registered trademarks of Newell Rubbermaid in the United States and/or other countries. Wi-Fi is either a trademark or a registered trademark of Wi-Fi Alliance in the United States and/or other countries. Bluetooth is either a trademark or a registered trademark of Bluetooth Special Interest Group. Windows is either a trademark or a registered trademark of Microsoft Corporation in the United States and/or other countries. All other trademarks are the property of Thermo Fisher Scientific inc. and its subsidiaries.

For U.S. Technical Support, please contact:

Thermo Fisher Scientific 3411 Silverside Road Tatnall Building, Suite 100 Wilmington, DE 19810 U.S.A.

Telephone: 302 479 7707

For International Support, please contact:

http://www.nanodrop.com/support

Contact your local distributor. For contact information go to:

http://www.nanodrop.com/Order.aspx

Toll Free: 1 877 724 7690 (U.S. & Canada only) E-mail: nanodrop@thermofisher.com

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or errorfree and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.

For Research Use Only. This instrument or accessory is not a medical device and is not intended to be used for the prevention, diagnosis, treatment or cure of disease.

WARNING Avoid an explosion or fire hazard. This instrument or accessory is not designed for use in an explosive atmosphere.

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Chapter 1 About the Spectrophotometer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Cuvette Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

USB-A port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

PR-1 Pedestal Reconditioning Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4

PV-1 Performance Verification Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Instrument Detection Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Chapter 2 Instrument Set up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Update Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Setting Up User Account Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

User Account Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Security Administration Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Chapter 3 Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

To make UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Best practices for UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . .13

UV-Vis Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Settings for UV-Vis Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Measure Chemometrics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Measure Chemometrics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Create Chemometrics Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Edit Chemometrics Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Chemometrics Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Settings for Chemometric Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

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Contents

Measure Custom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29

Measure using a Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Delete Custom Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Custom Method Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Manage Custom Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

Chapter 4 Learning Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

Micro-Volume Sampling—How it Works. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Set Up the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Measure a Micro-Volume Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Measure a Sample Using a Cuvette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Prepare Samples and Blanks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Basic Instrument Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

NanoDrop One Home Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

NanoDrop One Measurement Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74

NanoDrop One General Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90

Instrument Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .100

Data Deletion Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

PC Control Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

PC Control Home Screen overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

Control options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108

History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108

Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109

Chapter 5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111

Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

Cleaning the Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

Maintaining the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

Cleaning the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114

Reconditioning the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

Decontaminating the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119

Maintaining the Cuvette Sampling System . . . . . . . . . . . . . . . . . . . . . . . . . . . 121

Instrument Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122

Intensity Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122

Performance Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

Pedestal Image Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130

Chapter 6 Safety and Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131

Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132

Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133

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About the Spectrophotometer

NanoDrop OneC Spectrophotometer

Arm

Pedestal

Cuvette holder

NOTICE Locate the instrument away from air vents and exhaust fans to minimize

evaporation

The Thermo Scientific™ NanoDrop™ OneC is a compact, stand-alone UV-Visible spectrophotometer developed for micro-volume analysis of a wide variety of analytes. The patented sample retention system enables the measurement of highly concentrated samples without the need for dilutions.

The NanoDrop One NanoDrop QC PC Control software can be installed on a local PC and used to control the instrument and view data. The instrument can be connected to an optional printer with a USB cable or to a remote printer through an Ethernet connection or wireless network.

NOTICE Before operating a NanoDrop One instrument, please read the safety and

operating precautions and then follow their recommendations when using the instrument.

system comes with preloaded software and a touchscreen display.

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About the Spectrophotometer

Features

Touchscreen

TheNanoDrop OneC spectrophotometer features the patented micro-volume sample

retention system. The NanoDrop One

also features a cuvette holder for analyzing dilute

samples using standard UV-visible cuvettes.

TheNanoDrop OneC comes with a built-in, 7-inch high-resolution touchscreen preloaded with easy-to-use instrument control software. The touchscreen can slide left or right to accommodate personal preference, and tilt forward or back for optimal viewing

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Cuvette Holder

Instrument light path

About the Spectrophotometer

Cuvette holder

Features

USB-A port

The NanoDrop OneC includes a cuvette holder for measuring dilute samples, colorimetric assays, cell cultures and kinetic studies. The cuvette system has these features:

• extended lower detection limits

• 37 °C heater option for temperature-sensitive samples and analyses

• micro-stirring option to ensure sample homogeneity and support kinetic studies

For details, see Measure a Sample using a Cuvette.

Two more USB-A ports are located on instrument back panel

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About the Spectrophotometer

Accessories

This section lists the accessories included for use with the NanoDrop OneC.

PR-1 Pedestal Reconditioning Kit

Specially formulated conditioning compound that can be applied to the pedestals to restore them to a hydrophobic state (required to achieve adequate surface tension for accurate sample measurements). The kit includes conditioning compound and applicators. For more information, see

Reconditioning the Pedestals.

PV-1 Performance Verification Solution

Liquid photometric standard used to check instrument performance. For more information, see Performance Verification.

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Instrument Detection Limits

Measurement Location Pathlength (mm)

Pedestal 1.0 12.5

About the Spectrophotometer

Instrument Detection Limits

Upper Detection Limit (10 mm Equivalent Absorbance)

0.2 62.5

0.1 150

0.05 300

0.03 550

Cuvette 10 1.5

2 7.5

115

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About the Spectrophotometer

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Instrument Set up

Register Your Instrument

To register your instrument

1. Do one of the following:

On the website, locate NanoDrop One Registration and follow the instructions to register the instrument.

Update Software

Quickly and easily download and install the latest NanoDrop One software and release notes from our website. Follow the steps to update or upgrade the software on your local instrument and/or install or update the NanoDrop QC software on a personal computer (PC). An Internet connection is required to download software.

To install or update NanoDrop QC software on a PC

1. Insert the USB flash drive containing the installer software into an available USB port on

instrument. An Internet connection is required for registration.

– From any PC that is connected to the Internet, use any web browser to navigate to

our website.

your PC

2. Launch Start.exe. The software installer will run.

To install or update NanoDrop QC software on the instrument

1. Copy the .zip file with the new software from your computer to a USB storage device. Do not attempt to unzip the folder.

2. Insert the USB device into any USB port on the NanoDrop One

3. From the instrument Home screen, tap Settings > System > Update Software and choose the latest version of software.

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Instrument Set up

Setting Up User Account Control

User account control is managed using the Security Administration application. When you launch Security Administration, you will need to enter your Windows log-in information.

User Account Control

Launch the Security Administration application and select NanoDrop QC from the directory on the left to reveal Access Control and System Policies.

Access control

Access control is used to grant or deny individual users or groups access to protected features in the instrument application. Add and remove users and groups to the access list and set access rights using the drop-down for each entity.

System policies

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System Policies is used to set options that define the behavior of the client application. See

“Security Administration Policies.”

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Security Administration Policies

System policies allow you to assign data and method creation and deletion and editing privileges for users and groups.

Launch the Security Administration application and select NanoDrop QC -> System

Policies

You can add, delete, or edit policy groups and enable or disable the group’s users permission to delete data. When you are finished, select Save. Changes will take effect the next time NanoDrop QC is launched. Changes made to the Security Administration policies are applicable to the local PC only and will not affect other computers on the network.

Instrument Set up

Setting Up User Account Control

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Instrument Set up

Technical Support

For U.S./Canada Support, please contact:

Thermo Fisher Scientific 3411 Silverside Road Tatnall Building, Suite 100 Wilmington, DE 19810 U.S.A.

Telephone: 302 479 7707 Toll Free: 1 877 724 7690 (U.S. & Canada only) Fax: 302 792 7155 E-mail: nanodrop@thermofisher.com Website: www.thermoscientific.com/nanodrop

For International Support, please contact:

Contact your local distributor. For contact information go to:

http://www.nanodrop.com/Order.aspx

If you are experiencing an issue with your system, refer to the troubleshooting information. If the issue persists, contact us. If you are outside the U.S.A. and Canada, please contact your local distributor.

If your instrument requires maintenance or repair, contact us or your local distributor.

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Applications

Use the NanoDrop OneC to perform UV-Vis, Chemometrics, or your own custom measurements.

The UV-Vis application can be set up directly from the touchscreen and allows the instrument to function as a conventional spectrophotometer. Up to 40 wavelengths from 190 nm to 850 nm can be monitored and reported.

The Chemometrics application allows you to use your unique chemometrics method. The method is created using the NanoDrop QC PC Control software that is installed on your PC and then loaded into the instrument though a USB storage device. See the NanoDrop QC PC Control software to learn about Chemometric method features that are supported.

The Custom application provides additional flexibility for the method that you use with the instrument. See the NanoDrop QC PC Control software to learn about custom method features that are supported.

• Measure UV-Vis 12

• Measure Chemometrics 18

• Measure Custom 29

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Applications

Measure UV-Vis

The UV-Vis application allows the instrument to function as a conventional spectrophotometer. Sample absorbance is displayed on the screen from 190 nm to 850 nm. Up to 40 wavelengths can be designated for absorbance monitoring and inclusion in the report. Automatic pathlength adjustment and a single-point baseline correction can also be used.

Measures the absorbance of any sample at up to 40 wavelengths across the ultra-violet (UV) and visible regions of the spectrum.

Measure UV-Vis

Reported Results

Settings

Detection Limits

To make UV-Vis measurements

NOTICE

• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may cause permanent damage.

• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently damage the quartz fiber optic cables.

Before you begin...

Before taking pedestal measurements with the NanoDrop One instrument, lift the instrument arm and clean the upper and lower pedestals. At a minimum, wipe the pedestals with a new laboratory wipe. For more information, see Cleaning the Pedestals.

To measure a sample using the UV-Vis application

1. From the Home screen, select UV-Vis.

2. Specify up to 40 wavelengths to monitor (or you can specify them later if desired) and whether automated pathlength adjustment, analysis wavelength, and baseline correction will be used.

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Applications

Measure UV-Vis

3. Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm, or insert the blanking cuvette into the cuvette holder.

Tip: If using a cuvette, make sure to align the cuvette light path with the instrument light path.

4. Tap Blank and wait for the measurement to complete.

Tip: If Auto-Blank is On, the blank measurement starts automatically after you lower the arm. (This option is not available for cuvette measurements.)

5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove the blanking cuvette.

6. Pipette 1-2 µL sample solution onto the pedestal and lower the arm, or insert the sample cuvette into the cuvette holder.

7. Start the sample measurement:

– Pedestal: If Auto-Measure is On, lower arm; if Auto-Measure is off, lower arm and

tap Measure.

– Cuvette: Tap Measure.

When the sample measurement is completed, the spectrum and reported values are displayed (see the next section).

8. When you are finished measuring samples, tap End Experiment.

9. Lift the arm and clean both pedestals with a new wipe, or remove the sample cuvette.

Best practices for UV-Vis measurements

• Ensure the sample absorbance is within the instrument’s absorbance detection limits.

• Blank with the same buffer solution used to re-suspend the analyte of interest. The

blanking solution should be a similar pH and ionic strength as the analyte solution.

• Run a blanking cycle to assess the absorbance contribution of your buffer solution. If the

buffer exhibits strong absorbance at or near an analysis wavelength, you may need to choose a different buffer or application. See Choosing and Measuring a Blank for more information.

• For micro-volume measurements:

– Ensure pedestal surfaces are properly cleaned and conditioned.

– Ensure samples are homogeneous before taking a measurement. Avoid introducing

bubbles when mixing and pipetting.

– Follow best practices for micro-volume measurements.

– Use a 1-2 µL sample volume. See Recommended Sample Volumes for more

information.

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Applications

Measure UV-Vis

• For cuvette measurements (NanoDrop OneC instruments only), use compatible cuvettes

and follow best practices for cuvette measurements.

UV-Vis Reported Results

UV-Vis measurement screen

For each measured sample, this application shows the absorbance spectrum and a summary of the results. Here is an example as it appears on the local instrument display:

Menu of options;

tap to open

UV-Vis spectrum

Sample name; tap to edit

Absorbance at userdefined wavelength 1 (450 nm)

Tap to edit

Absorbance at user- defined wavelength 2 (623 nm)

Tap to edit

Tap to add

Tap row to

select sample and update spectrum; tap more rows to overlay up to five spectra. Press and hold sample row to view

Pinch and zoom to adjust axes; double-tap to reset

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Swipe screen left to view table with more measurement results

Tap to end experiment and export data

measurement details.

Drag tab down/up to see

more/less sample data

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Applications

Measure UV-Vis

Note Micro-volume absorbance measurements and measurements taken with nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.

UV-Vis reported values

The initial screen that appears after each measurement (see previous image) shows a summary of the reported values. To view all reported values, press and hold the sample row. Here is an example:

Application

User-defined wavelengths

Sampling method

Baseline correction wavelength

Sample name;

tap to edit

Absorbance at 635 nm

Date/time measured

Automated pathlength setting

Baseline correction absorbance

Absorbance at 450 nm

Absorbance at 623 nm

Note Scroll up to display absorbance values for any additional user-defined wavelengths.

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Applications

Measure UV-Vis

Below is an example of the measurement screen with reported values as it appears in the NanoDrop QC PC software:

Back to Home screen (ends experiment)

Sample name; select to edit

Run blank

Measure sample solution

Select to toggle Auto-Measure ON/OFF

Tips: Auto-Measure default is ON Auto-Blank default is OFF

Select to add absorbance at userdefined wavelengths

With sample selected, click and drag an area to zoom Right-click and select Autoscale to fit spectra to window

Tips:

Click sample row to select sample and update spectrum Shift-click multiple sample rows to overlay up to five spectra Click a sample and hover locations on spectra to view measurement values

End experiment and export data

Sample rows

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Settings for UV-Vis Measurements

To show the UV-Vis settings, from the Home screen, select UV-Vis.

Setting Available Options Description

Applications

Measure UV-Vis

Monitored wavelengths

Enter up to 40 wavelengths between 190 nm and 850 nm

Analytical Wavelength Any wavelength between

190 nm and 850 nm

Automated Pathlength On or Off

(affects pedestal measurements only)

User-defined wavelengths to be measured and reported at run time. Absorbance values for the first three entered wavelengths

are displayed in the measurement screen. To see absorbance values for 8 monitored wavelengths, swipe left in the measurement screen to show the Data table. To see all monitored wavelengths, press and hold a sample row to show the Sample

Details screen (scroll up to display absorbance values for any

additional user-defined wavelengths).

Note: If Baseline Correction is selected, all displayed absorbance values are the corrected values.

This is the wavelength the software will use to determine the pathlength selection.

Optional automated pathlength selection. Allows the software to use the optimal (shorter) pedestal pathlength for high concentration samples to help prevent detector saturation (see

Detection Limits for details).

• When selected, the shorter pathlength is used when any wavelength between 220 nm and 850 nm has 10 mm equivalent absorbance value of 12.5 or higher. For wavelengths between 190 nm and 219 nm the change to the shorter pathlength occurs when any wavelength in this range has a 10 mm equivalent absorbance value of 10 or higher.

• When deselected, the pedestal pathlength is restricted to 10 mm across all wavelengths.

Note: In either case, displayed absorbance values have been normalized to a 10 mm pathlength equivalent.

Baseline Correction On or off

Optional user-defined baseline correction. Can be used to

correct for any offset caused by light scattering particulates by Enter baseline correction wavelength in nm or use default value (750 nm)

subtracting measured absorbance at specified baseline correction

wavelength from absorbance values at all wavelengths in sample

spectrum. As a result, absorbance of sample spectrum is zero at

specified baseline correction wavelength.

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Applications

Measure Chemometrics

Make chemometric measurements.

Measure Chemometrics

Create Chemometric Method

Edit Chemometric Method

Reported Results

Settings

Detection Limits

Measure Chemometrics

The NanoDrop OneC model instrument can be used to make chemometric measurements.

Note The instrument arm can be up during cuvette measurements, which allows you to add reagents to the sample solution if desired.

To make chemometric measurements

NOTICE

• To prevent damage from spills, keep containers of liquids away from the instrument.

• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument ans may cause permanent damage.

Chemometric methods can only be created on a personal computer running the NanoDrop QC PC Control software. If you want to run a chemometric method and store the measurement results on the instrument, the method must also reside on the instrument. (This is the only way to run a chemometric method if your instrument is not connected to the computer with an Ethernet cable.)

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Applications

Measure Chemometrics

Load a chemometrics method

To measure a sample using a chemometrics method, you must first load the method onto the instrument.

To load chemometrics methods onto the instrument:

1. Export the method from the personal computer and copy the method file to the root of a portable USB device such as a memory stick.

Method files have a “.qmethod” filename extension.

2. Connect the USB device to one of the USB ports on the instrument.

3. From the Home screen, select the Chemometric Application icon

4. Use the list box at the top of the screen to indicate the USB port used.

5. Select Load Method.

A message box shows the NanoDrop One methods available on the selected USB device.

6. Select one or more method names in the Load Method box to select the methods to load.

7. Select Load.

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Applications

Measure Chemometrics

To measure a sample using the chemometrics application

1. From the Home screen, select the Chemometrics icon.

The Chemometrics Method Selection screen is displayed. If one or more chemometric methods exist in the currently selected Data Storage Location, they will be listed in the Select Method box. A description of the selected method appears in the Method Details box.

2. Select a method:

20 NanoDrop One

• select an existing method by selecting the method name in the Select Method box.

3. Select Run Method.

4. Follow the on-screen instructions to measure a sample.

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Applications

Measure Chemometrics

To measure a sample using the chemometrics application from the PC software

1. From the Home screen, select the Chemometric Method icon.

The Chemometrics Method Selection screen is displayed. If one or more chemometric methods exist in the currently selected Data Storage Location, they will be listed in the method selection pane below the search feature. Details of the selected method appears in the method details pane to the right.

2. Select a method:

• select an existing method by selecting the method name in the method selection pane.

3. Select .

Follow the on-screen instructions to measure a sample.

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Applications

Measure Chemometrics

Create Chemometrics Method

Chemometrics methods can be created only on the NanoDrop QC PC Control software. However, once the method is created, it can be saved in the NanoDrop One database on the local instrument, or in the NanoDrop QC database on the PC. To create a new Chemometrics method:

– From the NanoDrop QC software, select the Chemometric Method icon

– From the Chemometric Method Management pane, select

22 NanoDrop One

– Enter both a name and description for your chemometric method

– Select the quant method (.qnt fiile) you want to use

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– Adjust the method settings as desired

Applications

Measure Chemometrics

– Select Save

Note The method is saved in the currently selected Data Storage Location (local instrument or a connected PC).

– To run the method, select Run Method

Edit Chemometrics Method

Chemometrics methods can be edited only on the NanoDrop QC software. To edit an existing chemometrics method:

– From the NanoDrop QC software, select the Chemometric Method icon

– From the Chemometric Method Management screen, select the method you would

like to edit from the list of loaded methods.

– From the drop-down menu select Edit.

– Adjust the method settings as desired. You can select the components to be displayed

on the LC.

– Select Save

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Applications

Measure Chemometrics

Chemometrics Reported Results

Chemometrics method measurement screen (shown from Data Viewer)

For each measured sample, this application shows the absorbance spectrum and a summary of the results. Here is an example:

Menu of options;

tap to open

UV-visible spectrum

Analyte concentrations

Tap row to select sample and update spectrum; tap more rows to overlay up to five spectra.

Press and hold sample

row to view measurement details.

Drag tab down/up to

see more/less

Pinch and zoom to adjust axes; double-tap to reset

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sample data

Swipe screen left to view table with more measurement results

Note Micro-volume absorbance measurements and measurements taken with nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.

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Applications

Measure Chemometrics

Chemometrics method reported values

The initial screen that appears after each measurement (see previous image) shows a summary of the reported values. To view all reported values, press and hold the sample row. Here is an example:

Method name

Sampling method

Sample name;

tap to edit

Date/time measured

Analyte concentration

Method details

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Applications

Measure Chemometrics

Data Table

To see the data table, swipe the rate measurement screen (see above) to the left. Each row in the table shows the absorbance values at all user-defined wavelengths at a given stage and time. Scroll down to see measurement information that is out of view. The image below highlights the available features.

Measurement number

Sample name

Analyte concentration

Press and hold row to view measurement details

26 NanoDrop One

Swipe screen right to return

to Rate measurement screen

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Tap to end experiment and export data

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Applications

Measure Chemometrics

Measurement Details

To view details for a measurement, from the absorbance measurement screen or data table, press and hold the measurement row. Here is an example:

Print this

Application used

Return to

Sample nameSampling

method

Date/time measured

Component results

Method details (scroll up to view more)

Delete this

screen

previous screen

User-defined wavelengths

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Applications

Measure Chemometrics

Settings for Chemometric Measurements

Settings for chemometrics methods are defined by the quant method used. From the instrument Home screen, select Chemometrics, and select a mehtod from the list. The method details are displayed. Settings can be edited only from the NanoDrop QC software. In the PC Control software, you can select which components are displayed on the instrument and set significant figures for each component.

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Measure Custom

Runs a custom measurement method created using NanoDrop QC software.

Measure Custom Method

Delete Custom Method

Reported Results

Measure using a Custom Method

Use the Custom application to run a user-defined method created using the NanoDrop QC software running on a personal computer. For more information, see “Create Custom

Method” on page 35.

Applications

Measure Custom

To load a custom method

Custom methods can only be created on a personal computer running the NanoDrop QC software. If you want to run a custom method and store the measurement results on the instrument, the method must also reside on the instrument. (This is the only way to run a custom method if your instrument is not connected to the computer with an Ethernet cable.)

Load custom methods onto the instrument

1. Export the method from the personal computer and copy the method file to the root of a portable USB device such as a memory stick.

Method files have a “.method” filename extension.

Note Custom methods downloaded from the NanoDrop One website have a .zip filename extension and must be extracted using a third-party file decompression program before the software will recognize them as custom methods.

2. Connect the USB device to one of the USB ports on the instrument.

3. From the Home screen, select Custom Method.

4. Use the list box at the top of the screen to indicate the USB port used.

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Applications

Measure Custom

5. Select Load Method.

A message box shows the NanoDrop One methods available on the selected USB device.

6. Select one or more method names in the Load Method box to select the methods to load.

7. Select Load.

To measure using a custom method

NOTICE

• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may cause permanent damage.

• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently damage the quartz fiber optic cables.

Before you begin...

To measure a sample using a custom method using the local instrument interface

30 NanoDrop One

1. Make sure the method resides in same location as the database where you want to store the measurement results (see To Load a Custom Method for details).

2. From the Home screen, select Custom Method.

3. In the Select Method box, select to select the method to run.

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Applications

Measure Custom

Information about the selected method appears in the Method Details box.

4. Select Run Method.

5. Follow the on-screen instructions to measure a sample.

To measure a sample using a custom method using the PC software

1. Make sure the method resides in same location as the database where you want to store the measurement results (see To Load a Custom Method for details).

2. From the Home screen, select Custom Method.

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Applications

Measure Custom

3. In the method selection pane, select to select the method to run.

Information about the selected method appears in the method details pane.

4. Select .

5. Follow the on-screen instructions to measure a sample.

Delete Custom Method

– From Home screen, select Custom Method.

– In Select Method box, select a method to delete

– Select

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Custom Method Reported Results

Custom method measurement screen (shown from Data Viewer)

For each measured sample, this application shows the absorbance spectrum and a summary of the results. Here is an example:

Applications

Measure Custom

Menu of options;

tap to open

UV-visible spectrum

Analyte concentration

Tap row to select sample and update spectrum; tap more rows to overlay up to five spectra. Press and hold sample row to view measurement details.

Drag tab

Pinch and zoom to

adjust axes; double-tap to reset

Note Micro-volume absorbance measurements and measurements taken with nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.

Thermo Scientific NanoDrop One

Swipe screen left to view table with more measurement results

down/up to see more/less sample data

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Applications

Measure Custom

Custom method reported values

The initial screen that appears after each measurement (see previous image) shows a summary of the reported values. To view all reported values, press and hold the sample row. Here is an example:

Method name

Sampling method

Sample name;

tap to edit

Date/time measured

Analyte concentration

Method details

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Manage Custom Methods

The NanoDrop QC PC Control software is your tool for creating and managing custom methods, which contain user-defined settings that can be used to acquire data with the instrument. Custom methods can be made with or without standards.

Create Custom Method

Create method to be used for sample measurements with user-defined settings.

Create new custom method

– from the NanoDrop QC Home screen, select Custom Method

Applications

Measure Custom

Custom Method Icon

– in Manage Custom Methods screen, select and choose one of the

following:

– Formula (if your method will not have standards)

– Standard Curve (if your method will have standards)

– in the setup window, enter Method Name (this name appears in the Custom Setup

box on the instrument after the method has been transferred there)

– enter detailed Description of method, if desired

– specify how to calculate and report the method results:

– if method does not have standards, specify factor or extinction coefficient of

analyte (enter “1” to report absorbance measurements only)

– if method has standards, enter name and concentration of each standard and

select the curve fit type

– enter or choose remaining custom settings as needed

– choose Save

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Applications

Measure Custom

Note If appears at the bottom left of the screen instead of a green check mark icon, the method is invalid because it contains an error. Hover your mouse over the icon for suggested solutions.

if the method has a green check mark icon at the bottom, select Close to exit method setup

36 NanoDrop One

View or edit custom method

– select Custom Method (existing methods are listed in Select Method box along with

their type (formula or standards) and Description

– From the Custom Method Management screen, select the method you would like to

edit from the list of loaded methods.

– From the drop-down menu select Edit

– View and adjust the method settings as desired

– Select Save

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Applications

Measure Custom

Custom method settings

These settings are available for creating custom methods.

Setting Available Options

Result name Enter descriptive name for calculated concentration result (for example, “Polymer A

analysis”) and use adjacent drop down list to select appropriate unit. Result name appears as column heading for reported concentration value.

Measurement range Select spectral range in which method will acquire data.

Available options:

• Ultra-violet only (190 nm - 350 nm)

• Visible only (350 nm - 850 nm)

• Ultra-violet and visible (190 nm - 850 nm)

• Custom (specify starting and ending point in nanometers)

Analysis wavelength correction

Notes:

• If a Baseline correction and/or Analysis wavelength correction are used, make sure your selected spectral range includes your specified baseline correction and/or analysis correction wavelength.

• For micro-volume absorbance measurements and measurements taken with nonstandard (other than 10 mm) cuvettes, the spectra are normalized to a 10 mm pathlength equivalent.

Use this option to specify absorbance correction at analysis wavelength only. Available options:

• None. No correction at analysis wavelength.

• Single point. Enter wavelength for analysis correction. (Absorbance value at specified analysis correction wavelength is subtracted from absorbance value at analysis wavelength. Corrected value is used to calculate sample concentration.)

• Sloping baseline. Enter two wavelengths that define sloping baseline for analysis correction. (Absorbance value of sloping baseline at analysis wavelength is subtracted from absorbance value at analysis wavelength. Corrected value is used to calculate sample concentration.)

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Applications

Measure Custom

Setting Available Options

Factor or Extinction coefficient at 1 cm pathlength (Formula methods only)

Specify whether to use factor or extinction coefficient to calculate concentration result:

• User-defined factor. Enter factor for 1 cm pathlength and use adjacent drop down list to select appropriate unit. Equation below shows how factor is used to calculate sample concentration:

c = (A * f) / b

where:

c = analyte concentration A = absorbance in absorbance units (A)

f = factor (typically 1/ coefficient, or extinction coefficient) b = pathlength in cm (determined at measurement time, then normalized to 10 mm (1 cm) pathlength equivalent)

• Extinction coefficient and molecular weight. Enter extinction coefficient for 1 cm pathlength and use adjacent drop down list to select appropriate unit. Equation below shows how extinction coefficient is used to calculate sample concentration:

, where  = wavelength-dependent molar absorptivity

c = A / ( * b)

where:

c = analyte concentration A = absorbance in absorbance units (A)

 = wavelength-dependent molar absorptivity coefficient (or extinction

coefficient) b = pathlength in cm (determined at measurement time, then normalized to 10 mm (1 cm) pathlength equivalent)

Notes:

• Refer to product literature for information about factors and extinction coefficients for specific materials.

• To set up a method that reports absorbance measurements only, select Factor or Extinction Coefficient with the factor or extinction coefficient set to “1”.

• If specified unit for factor or extinction coefficient is based on mass (such as mg/mL) and specified unit for calculated result is based on molarity (such as pmol/µL) or vice versa, enter molecular weight and use adjacent drop down list to select appropriate unit.

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Setting Available Options

Applications

Measure Custom

Standards (Standard curve methods only)

Define the standards:

• Enter name and analyte concentration of each standard and a reference, if desired:

– Depending on the Curve Type setting, a standard curve can be generated

using two or more standards. (The software allows a reference and up to 7 standards.)

– All reference and standards solutions should be in the same buffer used to

resuspend the samples plus the same volume of reagent added to the samples.

– First standard can be a reference measurement. The reference solution

should contain none of the analyte of interest. (The reference measurement is not the same as a blank measurement.)

– Concentration values for standards can be entered in any order but the

standards must be measured in the order in which they were entered; however, best practice dictates that standards be measured from the lowest concentration of the standard analyte stock to the highest.

– Concentration range of the standards must cover the dynamic range of the

assay and the expected range of the unknown samples. Sample analyte concentrations are not extrapolated beyond the concentration of the highest standard.

• Select curve fit type.

Analysis wavelength (Standard curve methods only)

Specify type of equation used to create standard curve from standard concentration values. Available options:

– Linear: Draws the linear least squares line through all measured standards

(requires reference measurement and at least one standard)

– Interpolation: Draws a series of straight lines to connect all measured

standards (requires reference measurement ans at least one standard)

– 2

order polynomial: Draws th 2nd order least squares polynomial using all

measured standards (requires reference measurement and at least standards)

– 3rd order polynomial: Draws the 3rd order least squares polynomial using all

measured standards (requires reference measurement and at least three standards)

Monitor absorbance at specified wavelength (enter the wavelength in nanometers).

Note: The specified wavelength must fall within the selected measurement range.

The measurement results or the concentration will be calculated automatically using the absorbance value at the specified wavelength and applying the selected method type (factor or standard curve).

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Applications

Measure Custom

Setting Available Options

Baseline correction Select this option to correct offset caused by light scattering particulates by subtracting

the absorbance at a specified baseline point. Then specify wavelength for baseline correction.

Note: Software subtracts absorbance value at specified baseline correction wavelength from absorbance values at all wavelengths in sample spectrum. As a result, absorbance of sample spectrum is zero at specified baseline correction wavelength.

Automated pathlength Affects micro-volume measurements only.

• When Automated Pathlength is selected, software selects the optimal pathlength (between 1.0 mm and 0.03 mm) based on sample absorbance at the analysis wavelength. For example, when sample absorbance at the analysis wavelength is less than or equal to 12.5 (10 mm pathlength equivalent), the optimal longer pathlength is used. When sample absorbance is greater than 12.5, the optimal shorter pathlength is used. Recommended for samples that are highly absorbing at the analysis wavelength. (This option may cause reduced sensitivity when the sample spectra have a large absorbance peak that is not at the analysis wavelength.)

Note: When the analysis wavelength is between 190 nm and 219 nm, the optimal longer pathlength is used when sample absorbance is less than or equal to 10 (10 mm pathlength equivalent), and the optimal shorter pathlength is used when sample absorbance is greater than 10.

• When Automated Pathlength is deselected, the software uses a 1 mm pathlength regardless of the sample absorbance. This can cause detector saturation (resulting in jagged peaks) for highly absorbing samples (e.g., ~15 A at 10 mm pathlength equivalent).

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Applications

Measure Custom

Setting Available Options

Formula table (optional) Use the Formula table to specify additional reported results, such as a purity ratio, for

each sample.

Available options:

• Predefined. Select from a list of predefined formulas, which can be used as is or edited, and choose Add. The predefined formula is listed in the Formula Table.

• Add. Create formula for current method. Available options:

• Formula Name. Enter a name for the formula. After a measurement, the name is reported in Data Table and Sample Details screens.

• Formula. Enter valid formula (see below for rules and examples). After a measurement, the measured or calculated value is reported in Data Table and Sample Details screens.

• Unit. Enter unit for reported result. After a measurement, the unit is reported in Data Table and Sample Details screens.

• Edit. Edit selected formula for current method.

• Delete. Delete selected formula from current method.

Formula rules Custom formulas can include the following operators and functions:

• Path(). Returns sample pathlength in cm.

• A(nm). Returns sample absorbance at specified wavelength (for example, enter A(650) to add the measured absorbance at 650 nm to your equation).

• Operators: + (add), - (subtract), * (multiply), / (divide).

• Functions: Log(x), Pow(x,y).

Notes: Follow these additional rules for all languages:

• Use period “.” decimal separators for floating point and double-floating point numbers.

• Use comma “,” list separators (for example, “POW(2,8)”).

• Do not use comma “,” group separators for large numbers (for example, enter 1000 rather than 1,000).

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Applications

Measure Custom

Copy Custom Method

To create a custom method that is similar to an existing one, open the existing method, make your changes, then select Save As and enter a new name.

Copy custom method

You can now select the saved method and edit the Description and settings.

– from the Custom Methods screen, select a custom method

– from the drop-down menu choose Edit

– enter new Method name and Description

– select Save As

– Enter a filename for the method and click Save

Run Custom Method

If you want to run a custom method and store the measurement results on the instrument, the method must also reside on the instrument (see Load a Custom Method for details).

Export Custom Method

Export a custom method in order to run it and store the measurement results on the NanoDrop One

– from the Custom Methods screen, select a custom method

– from the kebab menu, choose Export (if method is invalid, an error message is

– choose Save (method is exported to method file (*.method filename extension) in

instrument.

displayed; errors must be fixed before method can be exported)

proprietary format)

To transfer the method to the NanoDrop One instrument, copy the method file to a USB memory device and then load the method (see Load a Custom Method for details)

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Import custom method

Import a custom method back to a computer running the NanoDrop One QC software in order to edit the method settings.

– from the Custom Methods screen, choose Import

– locate and select “.method” file

– choose Open (imported method is added to end of Select Method list)

Edit custom method

Edit a custom method in order to change the method settings.

– from Custom Methods screen, select a custom method from the list of available

methods

Applications

Measure Custom

– from the kebab menu, choose Edit

– edit method settings as desired

– choose Save

Delete custom method

– from Custom Methods screen, select a custom method from the list of available

– from the drop-down menu , choose Delete

after confirmation message, choose Ye s

methods

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Applications

This page is intentionally blank.

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Learning Center

Contents

• Micro-Volume Sampling—How it Works 46

• Set Up the Instrument 48

• Measure a Micro-Volume Sample 58

• Measure a Sample Using a Cuvette 63

• Prepare Samples and Blanks 66

• Basic Instrument Operations 71

• Instrument Settings 100

• PC Control Software 107

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Learning Center

Micro-Volume Sampling—How it Works

Surface Tension

Absorbance Spectrum

Sample Absorbance

Sample Concentration

Baseline Correction

Surface Tension

The NanoDrop OneC spectrophotometer uses surface tension to hold a small volume of sample between two pedestals. The patented sample retention system enables the measurement of highly concentrated samples without the need for dilutions.

A fiber optic cable embedded in the upper pedestal leads to a xenon light source. A second cable embedded in the lower pedestal leads to a detector. When the instrument arm is down, the sample forms a liquid column, essentially bridging the gap between the two fiber optic cables.

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Learning Center

Micro-Volume Sampling—How it Works

Absorbance Spectrum

The light passes through the liquid column to the detector, which generates a spectrum of absorbance versus wavelength. The spectrum shows the amount of light absorbed by the molecules of the sample at each measured wavelength.

Note: To prevent evaporation, which affects measurement accuracy, close the arm quickly after you finish loading a sample or blank.

The example at the left shows a typical absorbance spectrum taken of a nucleic acid sample. The spectrum is measured from 190 nm to 850 nm. The displayed range may vary for each application.

Sample Absorbance

Absorbance

intensity

--------------------------------------

log–=

intensityblank

sample

When the instrument is blanked, a reference spectrum is taken of the blanking solution and stored in memory. For each sample measurement, the sample intensities along with the blank intensities are used to calculate the total absorbance of the sample according to the equation at the left.

Baseline Correction

For some applications, the instrument can be set up to apply a baseline correction to each measurement to minimize any offset caused by light scattering particulates in the sample spectra. The correction subtracts the absorbance value at a reference wavelength that is close to zero from the absorbance value at each wavelength across the spectrum, essentially “anchoring” the spectrum to zero absorbance units at the reference wavelength.

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Learning Center

Set Up the Instrument

Power on/off

USB-A (2)

Ethernet

Power

Connect Power

CAUTION Avoid shock hazard. Each wall outlet used must be equipped with a ground.

The ground must be a noncurrent-carrying wire connected to earth ground at the main distribution box.

Connect the provided power cord to a grounded wall outlet. See “Power Cords” on page 137 for more information.

Connect an Accessory

To connect a compatible printer or other compatible accessory such as a USB keyboard and/or mouse to the instrument, use any USB port on the instrument (front, back-left or back-right). See Accessories for information about accessories compatible with the NanoDrop One instruments.

Set Up Bluetooth Connections

48 NanoDrop One

Use Bluetooth™ to connect the instrument to one or more Bluetooth (wireless) input devices such as a Bluetooth keyboard, mouse or barcode scanner.

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Learning Center

Set Up the Instrument

Note Make sure the device is labeled “Bluetooth” and not just “wireless.” All Bluetooth devices are wireless but not all wireless devices will run with Bluetooth.

Set up Bluetooth connections on the instrument

– from instrument Home screen, tap (Settings)

– tap System tab

– tap Bluetooth (if Bluetooth is disabled, button in upper right is set to “Off” and no

Bluetooth input devices are listed)

– tap Off button to enable Bluetooth connectivity (button turns blue, changes to “On”

and software automatically searches for any available Bluetooth input devices)

If no Bluetooth devices are found, after a few seconds the message “No nearby Bluetooth devices were found” is displayed

– to add a Bluetooth device, follow manufacturer instructions to pair the device (for

example, you may need to hold down a button) and tap Search For Devices on instrument)

device name should appear in Available Devices list

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Learning Center

Set Up the Instrument

– to pair device, tap its name in Available Devices list (a pairing request similar to the

following may be displayed)

– complete any instructions to pair the device

Note If your Bluetooth device does not pair, restart the device and then repeat the steps above to pair it with the instrument (you may also try turning Bluetooth off and back on). After a device is paired, it remains paired even after the instrument is restarted.

– tap Back (Bluetooth status is displayed at right of Bluetooth button)

50 NanoDrop One

– repeat steps above to add another Bluetooth device or tap Done to close Settings

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Learning Center

Set Up the Instrument

Deselect Bluetooth input device

You may want to stop using a Bluetooth device for input without disconnecting or unpairing it. This allows others to easily reselect and use the device for input. For example, if there are multiple connected and paired Bluetooth input devices such as a keyboard and a barcode scanner, follow these steps to select the devices to use or to deselect devices you don’t want to use:

– from instrument Home screen, tap

– tap System tab

– tap Bluetooth

– to deselect a paired Bluetooth device such as a

keyboard for input, tap its Profiles button

– deselect Use For Input by clearing it’s associated checkbox

– tap Paired Bluetooth Device in upper left to return to previous screen

– tap Back to return to System settings

– tap Done to close Settings

Note

• If no Bluetooth device is selected for input, the instrument relies on the integrated touchscreen keyboard for input.

• To select the device again, follow the steps above and select the device’s Use for Input checkbox.

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Learning Center

Set Up the Instrument

Disconnect Bluetooth device

– from instrument Home screen, tap

– tap System tab

– tap Bluetooth

– to disconnect paired Bluetooth device, tap its Profiles button

– tap Unpair

device is no longer listed under “Paired Devices” but remains in Available Devices list

– tap Back to return to System settings

– tap Done to close Settings

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Set Up Ethernet Connection

The instrument Ethernet port can be used to set up a wired connection between the instrument and either a personal computer (PC) or an active network wall jack.

If the instrument is connected to a network wall jack, you can export data files to a network location, for example, in order to transfer them to another computer. You can define multiple network paths that the operator can select when exporting data. See Export Settings for details.

Tools needed:

• Standard (straight through) Ethernet cable (CAT5e or newer is recommended)

Note If the computer is an older model, you may need a crossover Ethernet cable instead. Most newer model computers are designed to automatically detect and work with both cable types. However, a straight through cable will provide best performance.

Set up Ethernet connection

Learning Center

Set Up the Instrument

– from instrument Home screen, tap

– tap Networking tab

– tap Ethernet

– select an Ethernet option and choose OK.

• Direct connection to a PC. Select if you plan to connect an Ethernet cable

between the NanoDrop One

instrument and a personal computer.

• Connection to a network jack. Select if you plan to connect an Ethernet cable between the NanoDrop One

instrument and a network wall jack.

– connect one end of Ethernet cable to Ethernet port on instrument back panel

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Learning Center

Set Up the Instrument

Ethernet port

– connect other end of Ethernet cable to either the computer Ethernet port or an active

network wall jack

Set up Wireless Connections

Select Wi-Fi network on the instrument

– from instrument Home screen, tap (Settings)

– tap Networking tab

– tap Wi-Fi (if Wi-Fi is disabled, button in upper right is set to “OFF” and no wireless

networks are listed)

– tap button to enable Wi-Fi and display available Wi-Fi networks

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Learning Center

Set Up the Instrument

– select remote computer’s Wi-Fi network host and tap Connect (here is an example)

– tap Back to exit Wi-Fi setup (if the connection is successful, the instrument is

assigned an IP (Internet Protocol) address, which appears at the right of the Wi-Fi button as in the example below)

Note Some Wi-Fi networks may require an identity, password or other information before you can connect to them, or they may be anonymous (that is, you may have to search for them by name). For more information, see the system administrator at your work site.

– tap Done to exit Settings

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Learning Center

Set Up the Instrument

Assess Instrument Connectivity

Use the System Status icon at the top right of the instrument Home screen to quickly assess the instrument’s connectivity status including Bluetooth and Wi-Fi:

Tap to show connectivity status

Show connectivity status

– tap on instrument Home screen to open System Status box

Location of database where instrument is currently storing data (Local (instrument) or Connected PC)

– tap OK to exit System Status

Wi-Fi status

Bluetooth status

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Operating Specifications

The instrument operates reliably when the room environment meets these specifications:

• operating temperatures: 5 °C - 35 °C (41 °F - 95 °F)

• relative humidity (non-condensing): 20-80%

Locate the instrument away from air vents and exhaust fans to minimize evaporation.

Note If operating the instrument at the low end of the recommended humidity range, use adequate sample volume to avoid evaporation.

After the instrument is installed, you can leave it turned on.

Learning Center

Set Up the Instrument

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Measure a Micro-Volume Sample

The NanoDrop One spectrophotometer uses surface tension to hold a small volume of sample between two pedestals. The patented sample retention system enables the measurement of highly concentrated samples without the need for dilutions. Tap

here for details.

Supplies needed

• NanoDrop One or NanoDrop OneC spectrophotometer

• lint-free laboratory wipes

• calibrated precision pipettor (0–2 µL)

• sample material resuspended in appropriate buffer solution (see Preparing Samples)

• pure buffer solution for blanking instrument (see Choosing

and Measuring a Blank or watch multimedia training What is a blank?)

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Best practices for micro-volume measurements

Cleaning pedestals for daily operation

• Before first measurement, clean both pedestals with a new laboratory wipe.

• Run a blanking cycle to verify pedestals are clean.

• After each measurement, clean both pedestals with new wipe to prevent carryover.

• After each set of measurements, clean pedestals with DI H2O (see Clean

pedestals between users)

• Recondition pedestals periodically to maintain their hydrophobic property.

Learning Center

Measure a Micro-Volume Sample

Pipetting Samples

• Use recommended sample volumes to ensure proper liquid column formation.

• Use calibrated precision pipettor (0– 2 µL volume range) with well-fitting, low-retention precision tips to apply sample material to instrument for measurement.

If using low accuracy (0-10 µL) pipettor, use 2 µL sample volumes.

• Use new tip for each blank and sample aliquot.

• Use new aliquot of sample for each measurement.

• If solvents are used, make sure they are compatible with the pedestals. (see “Compatible Solvents” in Hazardous

Materials).

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Measure a Micro-Volume Sample

To measure a micro-volume sample

NOTICE

• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may cause permanent damage.

• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently damage the quartz fiber optic cables.

1. From the instrument Home screen, select an application: UV-Vis, Chemometric, or Custom Methods.

2. Lift the instrument arm and clean the upper and lower pedestals with new laboratory wipe.

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Learning Center

Measure a Micro-Volume Sample

3. Measure a blank:

– Pipette 1–2 µL blanking solution onto the lower

pedestal and quickly lower the arm

– Tap Blank and wait for the measurement to complete

Tip: If Auto-Blank is On, blank measurement starts automatically after you lower the arm.

– Lift the arm and clean both pedestals with a new

laboratory wipe

4. Measure the first sample:

– Pipette 1-2 µL sample solution onto the pedestal and

quickly lower the arm (see Recommended Sample

Volumes for more information)

Tap to end experiment

– Start the sample measurement:

– if Auto-Measure is On, lower arm

– if Auto-Measure is off, lower arm and tap Measure

– When the sample measurement is completed, the

spectra and reported values are displayed.

5. To measure another sample:

– Lift the arm

– Clean both pedestals with new wipe

– Load the next sample and quickly lower the arm

– Start the sample measurement

– Wait for the measurement to complete

The new spectrum replaces the previous one on the spectral display and the new reported values appear under the previous ones in the table. (Drag tab down to show both sets of data.)

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Measure a Micro-Volume Sample

6. When you are finished measuring samples:

– Tap End Experiment (see previous image)

– Enter an experiment name (tap Experiment Name box

to display keyboard), or leave the default experiment name

Tap to show keyboard; to close, tap Done key

Tap to measure more samples

Tap to end and save experiment

– Tap

– Lift the arm and clean both pedestals with a new wipe

If finished with the instrument for the day, clean the pedestals with DI H2O (see Clean pedestals between

users)

Acquired data are automatically saved in an experiment with the entered name. In the default configuration, experiments are stored in a database on the local instrument according to acquisition date, experiment name, application used and any assigned labels (see Manage identifiers on the instrument).

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Measure a Sample Using a Cuvette

The NanoDrop OneC spectrophotometer includes a cuvette holder for measuring dilute samples, colorimetric assays, cell cultures and kinetic studies. The cuvette system offers an extended lower detection limit and an optional 37 °C heater and micro-stirrer.

Supplies needed

• NanoDrop OneC spectrophotometer

Learning Center

Measure a Sample Using a Cuvette

• lint-free laboratory wipes

• two compatible cuvettes

• sample material resuspended in appropriate buffer solution (see Preparing Samples)

• pure buffer solution for blanking instrument (see

Choosing and Measuring a Blank or watch multimedia

training What is a blank?)

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Measure a Sample Using a Cuvette

Best practices for cuvette measurements

• The instrument arm can be up or down for cuvette measurements.

• Use 10 mm, 5 mm, 2 mm or 1 mm cuvettes up to 48 mm tall.

• Clean and dry cuvette after each measurement.

• Use cuvettes that are free of scratches and avoid fingerprints which may affect results.

• Use quartz cuvettes or UV-grade plastic cuvettes to measure samples with analysis wavelengths in the UV range (<340 nm).

Cuvette holder

Instrument light path

• Micro, semi-micro, and ultra-micro cuvettes should be masked.

• Fill cuvettes with enough blanking or sample solution to cover instrument optical path (2 mm sample beam is

8.5 mm above cuvette bottom).

• Lift instrument arm and make sure cuvette holder is free of debris.

• When inserting quartz or masked plastic cuvettes, align cuvette light path with instrument light path.

To measure a sample using a cuvette

NOTICE

• To prevent damage from spills, keep containers of liquids away from the instrument.

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• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument ans may cause permanent damage.

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Learning Center

Measure a Sample Using a Cuvette

1. From the Home screen, select (Settings)

2. Specify the cuvette options:

– Select General

– Select Use Cuvette

– Set Pathlength to pathlength (width) of

cuvette (see cuvette manufacturer for specifications)

Cuvette holder

– Set stirrer and heater if desired

– Select Done

See General settings for details.

3. From the Home screen, select an application

4. Measure a blank:

– Fill clean, dry cuvette with enough blanking

solution to cover instrument optical path

– Lift instrument arm and insert blanking

cuvette into cuvette holder, making sure to align light path of cuvette with light path of instrument

– Tap Blank and wait for the measurement to

complete

Instrument light path

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Prepare Samples and Blanks

5. Measure a sample:

– Fill clean cuvette to same height with sample

solution

– Replace blanking cuvette with sample

cuvette, making sure to align light paths

– Tap Measure

– Wait for measurement to complete

– Remove cuvette

– Clean cuvette according to manufacturer

specifications

Preparing Samples

• Isolate and purify samples before measuring them with the instrument. Commercial sample isolation kits are available for these purposes, or use an in-house protocol. After purification, analyte of interest is typically dissolved in aqueous buffer solution before it is measured.

Tip: Any molecule that absorbs light at analysis wavelength will contribute to total absorbance value used to calculate sample concentration.

• Ensure final analyte concentration is within instrument’s absorbance detection limits.

• For micro-volume measurements, gently (but thoroughly) vortex each sample before taking a measurement.

Avoid introducing bubbles when mixing and pipetting. For more information, watch multimedia training Effects of Bubbles in Samples.

Note Samples dissolved in extremely volatile solvent such as hexane may work best with

cuvette sampling option (NanoDrop One

instruments only).

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Choosing and Measuring a Blank

The buffer used to resuspend a sample analyte can contribute absorbance. Blanking minimizes any absorbance contribution due to the buffer components from the sample measurement. The resulting sample spectrum represents the absorbance of only the analyte of interest. For more information, watch the multimedia training What is a blank?

For best results:

• For most applications, blank with the same buffer solution used to resuspend the analyte of interest. The blanking solution should be a similar pH and ionic strength as the analyte solution. For details, see “To measure samples” in the application used.

• Measure new blank before each set of samples. It is not necessary to blank the instrument before each sample measurement unless the samples are dissolved in different buffer solutions.

Learning Center

Prepare Samples and Blanks

• Measure a new blank every 30 minutes.

• Run a blanking cycle to assess the suitability of your blanking solution before using it to perform sample measurements. For a quick demonstration, watch the multimedia training

Evaluating a Blanking Solution for Suitability.

The resulting spectrum should vary no more than 0.04 A (10 mm equivalent) across the spectrum, especially at the analysis wavelength as in the example at the right.

If the resulting spectrum is greater than 0.04 A around the analysis wavelength, that buffer solution may interfere with the sample analyses, especially for low concentration samples. See below for details.

Good blanking buffer (measured abs < 0.04)

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Prepare Samples and Blanks

Problems associated with blanking

• Residual sample was left on pedestal or in cuvette before blank measurement was performed. (Resulting sample spectra may exhibit negative absorbance values, indicating blank had more absorbance than sample in that region of spectrum.)

• Blank measurement exhibits higher absorbance than unknown sample at analysis wavelength. (If buffer used as blank differs in composition from that used to resuspend sample, measurement results will be incorrect.)

• Sample was inadvertently used to blank instrument. (Resulting sample spectra may exhibit negative absorbance values or, in some cases, resemble a mirror image of a typical pure nucleic acid or protein spectrum as in example at right.)

Solutions for blanking problems

• Thoroughly clean and/or recondition both

pedestals and then:

– rerun blanking cycle, or

– measure new blank using new aliquot of

appropriate buffer solution, then measure new aliquot of unknown sample

Protein sample solution used to blank instrument results in “mirror image” spectrum

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Run a Blanking Cycle

Run a blanking cycle to verify the following:

• instrument is operating normally (with flat baseline)

• pedestals are clean (i.e., no dried-down sample material on pedestals)

• absorbance contribution of buffer solution you plan to use for sample analyses

Supplies needed

• lint-free laboratory wipes

• calibrated precision pipettor (0–2 µL)

• buffer solution for evaluation

Learning Center

Prepare Samples and Blanks

To run a blanking cycle

For quick demonstration, watch multimedia training Evaluating a Blanking Solution for

Suitability.

NOTICE

• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may cause permanent damage.

• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently damage the quartz fiber optic cables.

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Prepare Samples and Blanks

1. From the Home screen, select an application name.

2. Lift the instrument arm and clean the upper and lower pedestals with new laboratory wipe.

3. Measure a water blank:

– Pipette exactly 1 µL deionized water

(DI H lower the arm.

– Tap Blank and wait for the

measurement to complete.

– Lift the arm and clean both pedestals

with new laboratory wipe.

4. Measure the buffer solution:

– Pipette 1-2 µL buffer solution onto the

pedestal and lower the arm.

O) onto the lower pedestal and

Example spectrum of buffer suitable for Protein A280 protein quantification

– Start the sample measurement:

– if Auto-Measure is On, lower arm

– if Auto-Measure is off, lower arm

and tap Measure

– Wait for measurement to complete.

The resulting spectrum should vary no more than 0.04 A from the baseline at the analysis wavelength.

If your spectrum does not meet these criteria, repeat steps 2–4.

If spectrum is still outside specifications, see Solutions for Blanking Problems.

5. When you are finished with the blanking cycle, tap End Experiment.

6. Lift the arm and clean both pedestals with a new wipe.

Example spectrum of buffer unsuitable for Protein A280 protein quantification

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Basic Instrument Operations

• NanoDrop One Home Screen

• NanoDrop One Measurement Screens

• Open Data Viewer

• NanoDrop One General Operations

NanoDrop One Home Screen

These operations are available from the NanoDrop One Home screen.

Learning Center

Basic Instrument Operations

System StatusApplications

Data Viewer Instrument

Settings

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Applications

System Status

The NanoDrop QC software offers several configurable applications, which gives users full control of the measurement. See“Applications” on page 11 for detailed information about each available application.

Tap on the instrument Home screen to open the system status box. Here is an example:

72 NanoDrop One

The available information is described below.

Instrument type Instrument model (NanoDrop One

)

Serial number Instrument serial number

Instrument status Current status of the instrument

Data storage location Indicates location of database set where instrument is currently

storing data. These options are available:

• Local (instrument)

• Connected PC* (personal computer connected through

Ethernet cable or wireless network)

* the Ethernet and wireless options listed above also store data on the instrument as a backup.

Wi-Fi status Status of WiFi connections for the instrument (“Connected

to...”, “Enabled and not connected” or “Disabled”)

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Data Viewer

Learning Center

Basic Instrument Operations

Bluetooth status Status of Bluetooth connections for the instrument

(“Connected to...”, “Enabled-[list of any paired devices]” or “Disabled”)

Software package version Version of instrument operating software installed

Platform release Version of instrument platform software installed

Firmware version Version of instrument firmware installed

Android release Version of customized Android operating system software

installed

Android version Version of Android operating system software installed

Tap on the Home screen to view any data acquired earlier today, last week, last month, last six months, last year or in a specific date range. See “Open Data Viewer” on page 82 for more information about the Data Viewer on the instrument.

Instrument Settings

Tap on the Home screen to access instrument settings for software updates, cuvette sampling, networking and more. See “Instrument Settings” on page 100 for detailed information about all available instrument settings.

Instrument Diagnostics

Tap on the Home screen to verify instrument operation. Instrument diagnostics should be run periodically according to the recommended maintenance schedule. See

“Instrument Diagnostics” on page 122 for information about how to run the available

instrument diagnostics.

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NanoDrop One Measurement Screens

These operations are available from any measurement screen within an Application.

Menu of options; tap to open

Sample pathlength

Sample name; tap to edit

Measurement results; see

Applications for details

Measurement alert; tap to learn more

Tap row to select sample and update spectrum; tap

more rows to

UV absorbance

overlay up to five

spectrum for

spectra. Press

selected sample

and hold sample

row to view sample details.

Drag tab

Tap to measure

blank solution

Pinch and zoom to adjust axes

Tap to measure

sample solution

AutoMeasure

Tap to end experiment and export data

Page control; swipe screen left to view table with more measurement results

Tap in any measurement screen to see the available menu options.

Home Return to NanoDrop One Home screen

[application] Setup View or change settings for selected application

Settings View or change instrument settings

Note: The Dye/Chrom. Editor and Protein Editor tabs appear in Settings only when the Settings tab is opened from the NanoDrop One Home screen or the Data Viewer.

Print Print selected measurement results

down/up to see more/less sample data

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Sample Name

Learning Center

Basic Instrument Operations

Tap the Sample Name field in any measurement screen to edit the sample name.

When Auto-Naming is On (see General Settings), each sample is automatically assigned a sample name using the default base name followed by a unique number starting with “1.” The first time this appears is after the first blank measurement and before the first sample measurement in each experiment as shown below.

Default sample name; tap to edit

In this example, the first sample would be named “Sample 1” followed by “Sample 2,” etc. You can edit the default base name and overwrite any sample name.

Note If you edit the sample base name during an experiment when Auto-Naming is selected, the assigned sample ID numbers restart.

Edit default sample base name

After you measure a blank and before the first sample is measured:

– tap Sample Name field to display keyboard

– enter new base name

– tap Done key

Edit sample name

– from Home screen, tap to open Data Viewer

– select experiment

– swipe left to show data table

– press and hold sample name to show Sample Details box

– tap Sample Name field to display keyboard

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Basic Instrument Operations

Measurement Results

The types of results that appear in the measurement screens depend on the selected application. For details, see the reported results section of that application in this guide:

– enter new sample name

– tap Done key to close keyboard

– tap OK to close Sample Details box

Applications > [application group] > Measure [application name] > Reported Results

Absorbance Spectrum

For each measured sample, each application shows the UV or UV-visible absorbance spectrum and a summary of the results. The vertical axis shows absorbance in absorbance units (A). The horizontal axis shows wavelength in nm. Here is an example for a chemometric method.

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Sample Pathlength

Learning Center

Basic Instrument Operations

All applications display the sample pathlength along the spectrum’s vertical axis. Micro-volume absorbance measurements and measurements taken with nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent. Here is an example.

Sample pathlength

Blank Button

Measure Button

Tap Blank to measure a blank for the selected experiment.

A blank must be measured before each group of similar samples. The blank solution is typically the pure buffer that was used to resuspend the sample. For more information, see

Choosing and Measuring a Blank.

Tap Measure to measure a sample for the selected experiment.

Samples must be properly isolated and prepared before they can be measured with the instrument and the concentration must be within the instrument’s absorbance detection limits. For more information, see Preparing Samples. and Measure a Micro-Volume Sample or

Measure a Cuvette Sample and Absorbance Detection Limits.

Note The Measure button is enabled after a valid blank measurement is completed.

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Basic Instrument Operations

Auto-Measure and Auto-Blank Options

Speed up sample analysis with the NanoDrop One Auto-Measure and Auto-Blank features, which cause the instrument to start the measurement immediately after you lower the instrument arm. These options eliminate the need for repetitive Measure or Blank operations for large batches of samples.

Note Auto-Measure and Auto-Blank are available for micro-volume measurements only.

Auto-Measure

To select or deselect Auto-Measure, from any sample measurement screen, tap the On or Off button at the right of the Measure button.

Auto-Blank

To select or deselect Auto-Blank, from any blank measurement screen, tap the On or Off button at the right of the Blank button.

End Experiment Button

Tap End Experiment when you are ready to name and save your experiment, add a label to help you locate the experiment later or export the data.

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Learning Center

Basic Instrument Operations

Note The End Experiment button is enabled after the first sample measurement is completed.

After you tap End Experiment, the End Experiment box is displayed:

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Basic Instrument Operations

Available options:

Experiment Name Enter a name for this group of measurements. The

measurement results are saved in the selected database location using the entered experiment name.

Add Identifier Enter a descriptive label to help you find this experiment later or

to associate it with another experiment (see Manage identifiers

on the instrument for details).

Tap the Add Identifier box to display a keyboard to enter the label text.

Tap the Add Identifier button to add the label; tap the Done key to close the keyboard.

Export Data Select an available location for exporting the measurements in

this experiment. Experiments can be exported to a USB device connected to any USB port on the local instrument (front, back-left or back-right) or to a network location.

Export Button Allows you to select a file format for exporting the

measurements in this experiment and then export the data to a USB device or network. Available export file formats:

• comma-separated values spreadsheet (.csv) file

• tab-separated values spreadsheet (.tsv) file (spectral data only)

• NanoDrop QC (.sql) file

The filename is the entered experiment name (see above). The file is stored in a folder named “NanodropOne” followed by the instrument serial number. (Use System Status to view your instrument serial number.)

Return To Experiment button

Close the End Experiment box and display the results for the most recent measurement. From there you can add measurements to the current experiment and save it later.

Print button Print measurement results for current experiment

End Experiment button End the experiment and save the measurement results using the

entered experiment name. The experiment is saved in the selected database location.

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Sample Details

Learning Center

Basic Instrument Operations

Press and hold a sample row in any measurement screen or data table to show the sample details, which include all available measurement results and associated details for the selected sample. Here is an example:

Information about the measured values displayed in Sample Details is provided in this Help system, under the application used to acquire the data.

Note You can also edit the sample name from the Sample Details box.

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Data Table

Swipe left in any measurement screen to see the data table for the current experiment. The data table contains the measurement results for all samples in the experiment. The image below highlights the available features.

Menu of options;

tap to open

Sample name;

tap to edit

Measurement results; see

Applications for details

Measurement

alert; tap to

learn more Tap row to

select sample; Press and hold row for sample

details

Application used

Open Data Viewer

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Page control; swipe screen right to return to measurement screen

Whether you collect one sample or many in a row, after you choose End Experiment, the acquired data are automatically saved in an experiment with an experiment name. In the default configuration, experiments are stored in the NanoDrop One database on the local instrument according to acquisition date, experiment name, application used and any assigned labels.

Use the Data Viewer to open the database on the local instrument in order to view acquired spectra and associated data from any experiment at any time.

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Learning Center

Basic Instrument Operations

Open instrument database of measurement results

– to open NanoDrop One

database on instrument, tap (Data Viewer) on

instrument Home screen

Tap in the Data Viewer to see the available menu options.

Home Return to NanoDrop One Home screen

Settings View or change instrument settings

Import Import data from a USB flash drive

Disk Status View remaining space available for storing measurement data on

the instrument

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Basic Instrument Operations

Search Experiment Database

Tap Search in the Data Viewer to search the selected database for an experiment or to change the time range or other search filters. The database is filtered using the current settings in the Search box. Filters include time range, application type and any user-defined labels (see

Manage Identifiers for information about adding and deleting labels). Here is an example:

Tap to change time range filter

Change a filter and tap OK to display updated list of experiments

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Tap to select or deselect application filters

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Export Selected Experiments

Use Select in the Data Viewer to select experiments to be exported.

Export selected experiments

– open the Data Viewer and tap Select

Learning Center

Basic Instrument Operations

– tap row to list experiments acquired on that date, or use Search feature to find

experiment

– tap to select one or more experiments to export (tap again to deselect an experiment;

to select all experiments in database, select All)

– tap Export

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Learning Center

Basic Instrument Operations

– set Export Data to an available export location (front, back-left or back-right USB

port, or a network location) and select Export

– select one or more formats to export to (see “Export Selected Experiments” in

General Operations for details) and tap Export

– after “Export Success” message, tap OK

Delete Selected Experiments

Use Select in the Data Viewer to select experiments to be deleted.

Delete selected experiments

– tap row in Data Viewer to list experiments acquired on that date, or use Search

feature to find desired experiment

– tap Select

– tap to select one or more experiments to delete (tap again to deselect an experiment)

– tap Delete and OK

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NOTICE Deleted data cannot be recovered.

Open Experiment and View Associated Data

Use the Data Viewer to locate and open any experiment to see the measurement data it contains.

Open an experiment

– tap row in Data Viewer to list experiments acquired on that date,

or use Search feature to find desired experiment

Learning Center

Basic Instrument Operations

– tap experiment name to open the experiment

Here is an example:

One experiment measured on this date

Tap to open this experiment; press and hold to view or edit experiment details such as experiment name

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The Data Viewer provides measurement data as spectral data and data tables, similar to what you see after you complete a measurement.

Note The data shown are dependent upon the application used to measure the samples (nucleic acids in these examples). For more information, see the application details.

Spectral data—

After you open an experiment, the software shows the UV or UV-visible absorbance spectrum and a summary of the associated data for the first sample measurement, much like it appears during a measurement. The image below describes the available features.

Menu of options;

tap to open

Selected experiment

UV spectrum for selected sample

Measurement results; see

Applications for details

Measurement

alert; tap to

learn more

Tap row to select sample and update spectrum; tap more rows to overlay up to five spectra. Press and hold sample row to view sample details.

Selected application

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Drag tab

Pinch and zoom

to adjust axes

Page control; swipe screen left or

right to view next or previous screen

down/up to

see more/less sample data

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Learning Center

Basic Instrument Operations

Data Table—

Swipe left in any Spectral Data screen to see the data table for the current experiment. The data table contains the measurement results for all samples in the experiment. The image below describes the available features.

Menu of options;

tap to open

Selected experiment

Measurement results; see

Applications for details

Tap to select unit

Tap row to select sample; Press and hold row for sample details

Measurement

alert; tap to

learn more

Application used

Tap from any Spectral Data or Data Table screen to see the available menu options.

Home Return to NanoDrop One Home screen

Manage Identifiers Add or delete labels for selected experiment to make it easier to

Export Export selected experiments

Print Print plot or data table for selected measurement results; if no

Settings View or change instrument settings

Disk Status View remaining space available for storing measurement data on

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Page control; swipe screen right to view previous screens (2)

find (see Manage identifiers on the instrument)

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Basic Instrument Operations

NanoDrop One General Operations

These operations are available from any measurement screen or from the Data Viewer.

Manage Identifiers (on the instrument)

You can add one or more “identifiers” (i.e., labels or metadata tags) to an experiment to make the experiment easier to find. Labels can be added from the NanoDrop One software running on the instrument, or from the NanoDrop QC software installed on a personal computer (see

Manage Identifiers on a PC).

Use the Data Viewer to add labels to experiments, assign existing labels, view assigned labels and remove or delete labels on the instrument. You can filter the list of experiments in the Data Viewer based on one or more user-defined labels.

Label new experiment when you save it

– after the last sample has been measured, tap

– in End Experiment box, tap Add Identifier field

– use displayed keyboard to enter label and tap

– tap Done key

– tap End Experiment

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Label experiment in Data Viewer

– from Home screen, tap to open Data Viewer

– tap to open an experiment

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– tap and choose Manage Identifiers

– in Manage Identifiers box, tap Add Identifier field

– use displayed keyboard to enter label and tap

– tap Done key

– tap OK

View assigned labels for an experiment

– from Home screen, tap to open Data Viewer

– press and hold selected experiment to see Experiment Details

Find labeled experiments

Learning Center

Basic Instrument Operations

– from Home screen, tap to open Data Viewer

– tap Search

– in Search box, select date range, select application (only applications that have

associated data are shown), select one or more identifiers from scrollable list and tap

Remove a label

– from Home screen, tap to open Data Viewer

– tap to open an experiment

– tap and choose Manage Identifiers

– in Manage Identifiers box, select label and tap .

– tap OK

Edit Experiment Name

You can edit the experiment name when you save the experiment or afterwards from the Data

Viewer.

Edit experiment name at end of experiment

– when finished measuring samples, tap

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Basic Instrument Operations

– enter a name for this group of measurements in the Experiment Name box

– tap End Experiment

Edit experiment name from Data Viewer

– from Home screen, tap to open Data Viewer

– tap row to list experiments acquired on that date, or use Search feature to find

experiment

– press and hold experiment name to open experiment details box

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– tap Experiment Name field to display keyboard

– enter new experiment name

Learning Center

Basic Instrument Operations

– tap Done key to close keyboard

– tap OK to close Experiment Details box

Export Selected Experiments

You can export measurement data when you save the experiment or afterwards from the Data

Viewer.

Note Data exported during a save are still saved to a database (local or remote, depending on the Data Storage setting; see Select location for saving or viewing collected data for more information).

Measurement data can be exported in four formats:

• as comma-separated values (.csv) files containing the measurement results and details for each exported experiment

• as tab-separated values (.tsv) files containing x,y coordinates for every spectral data point for each exported experiment

• as NanoDrop QC (.sql) files containing spectra and measurement results for each exported experiment

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Use any spreadsheet or word processing application to open a CSV or TSV file. Here is an example of several sample measurement results in CSV format:

Note The types of data exported are dependent upon the application used to measure the samples (nucleic acids in this example). For more information, see the application details.

Data can be exported to a USB device connected to any USB port on the local instrument (front, back-left or back-right) or to a network location. If you select multiple experiments for export, each exported experiment has a corresponding file. The filenames are the same as the

experiment names. The files are stored in a folder named “NanodropOne” followed by the

instrument serial number. (Use System Status to view your instrument serial number.)

Export data at end of experiment

– when finished measuring samples, tap

– from End Experiment box, set Export Data to an available export location (front,

back-left or back-right USB port, or a network location)

– tap

– from Export box, select one or more formats to export to (see above for details) and

tap Export

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– after “Export Success” message, tap OK

– tap End Experiment

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Export data from Data Viewer

– from Home screen, tap to open Data Viewer

– tap Select

– tap row to list experiments acquired on that date, or use Search feature to find

experiment

– tap to select one or more experiments to export (tap again to deselect an experiment;

to select all experiments in database, select All)

– tap Export

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– set Export Data to an available export location (front, back-left or back-right USB

port, or a network location) and tap Export

– select one or more formats to export to (see above for details) and tap Export

– after “Export Success” message, tap OK

Delete Selected Measurements

You can delete selected sample measurements from any experiment, or all the measurements in the database.

NOTICE Deleted data cannot be recovered.

Delete data from any measurement screen

– press and hold sample row to open Sample Details box

– tap

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Thermo Scientific Nanodrop Onec User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Contents

About the Spectrophotometer

Features

Touchscreen

Cuvette Holder

USB-A port

Accessories

PR-1 Pedestal Reconditioning Kit

PV-1 Performance Verification Solution

Instrument Detection Limits

Instrument Set up

Register Your Instrument

Update Software

Setting Up User Account Control

User Account Control

Security Administration Policies

Technical Support

Applications

Measure UV-Vis

Measure UV-Vis

To make UV-Vis measurements

Best practices for UV-Vis measurements

UV-Vis Reported Results

Settings for UV-Vis Measurements

Measure Chemometrics

Measure Chemometrics

Create Chemometrics Method

Edit Chemometrics Method

Chemometrics Reported Results

Settings for Chemometric Measurements

Measure Custom

Measure using a Custom Method

Delete Custom Method

Custom Method Reported Results

Manage Custom Methods

Learning Center

Micro-Volume Sampling—How it Works

Set Up the Instrument

Measure a Micro-Volume Sample

Measure a Sample Using a Cuvette

Prepare Samples and Blanks

Basic Instrument Operations

NanoDrop One Home Screen

NanoDrop One Measurement Screens

NanoDrop One General Operations