Page 1
Thermo Scientific
Multidrop® 384
User Manual
Rev. 3.4
Page 2
Page 3
Thermo Scientific
Multidrop
®
384
User Manual
Rev. 3.4, Cat. no. 1507010
Page 4
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 2
Thermo Scientific Multidrop 384, Cat. no. 5840150 or 5840157
User Manual Rev. 3.4; October 2008, Cat. no. 1507010
CONTENTS
1 SAFETY SYMBOLS AND MARKINGS........................................................................................... 6
2 ABOUT THE USER MANUAL ........................................................................................................ 7
3 INTRODUCTION TO THE MULTIDROP 384.................................................................................. 8
4 FUNCTIONAL DESCRIPTION ...................................................................................................... 10
5 INSTALLATION............................................................................................................................. 15
6 ROUTINE OPERATION................................................................................................................. 21
7 MAINTENANCE............................................................................................................................. 36
8 TECHNICAL SPECIFICATIONS ................................................................................................... 60
9 TROUBLESHOOTING................................................................................................................... 66
10 WARRANTY CERTIFICATE ......................................................................................................... 71
11 ORDERING INFORMATION ......................................................................................................... 72
12 GLOSSARY AND ABBREVIATIONS ........................................................................................... 75
13 INDEX............................................................................................................................................. 77
14 TABLE OF FIGURES .................................................................................................................... 81
APPENDIX 1. THERMO SCIENTIFIC MULTIDROP 384 BRIEF USER’S GUIDE.............................. 83
APPENDIX 2. THERMO SCIENTIFIC MULTIDROP 384 FEEDBACK FORM.................................... 84
APPENDIX 3. ADDRESSES................................................................................................................. 85
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Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 3
TABLE OF CONTENTS
1
SAFETY SYMBOLS AND MARKINGS........................................................................................... 6
2 ABOUT THE USER MANUAL ........................................................................................................ 7
3 INTRODUCTION TO THE MULTIDROP 384.................................................................................. 8
3.1 INTENDED USE ............................................................................................................................ 8
3.2 PRINCIPLE OF OPERATION............................................................................................................ 8
3.3 ADVANTAGES OF USING THE MULTIDROP 384 ............................................................................... 9
4 FUNCTIONAL DESCRIPTION ...................................................................................................... 10
4.1 INSTRUMENT LAYOUT................................................................................................................. 10
4.1.1 Front view ........................................................................................................................ 10
4.1.2 Back view......................................................................................................................... 13
4.2 MULTIDROP 384 MICROPLATE DISPENSER................................................................................... 14
5 INSTALLATION............................................................................................................................. 15
5.1 INSTALLATION CHECK LIST.......................................................................................................... 15
5.2 WHAT TO DO UPON DELIVERY..................................................................................................... 15
5.2.1 How to unpack................................................................................................................. 15
5.2.2 Checking delivery for completeness................................................................................ 15
5.2.3 Checking for damage during transport ............................................................................ 16
5.2.4 Environmental requirements............................................................................................ 16
5.2.5 Things to avoid ................................................................................................................ 16
5.2.6 Technical prerequisites.................................................................................................... 16
5.3 SETUPS BEFORE YOU PUT THE INSTRUMENT INTO OPERATION...................................................... 17
5.3.1 Installation procedure ...................................................................................................... 17
5.3.2 How to ensure startup ..................................................................................................... 18
5.4 OPERATIONAL CHECK ................................................................................................................ 19
5.5 OPERATING PRECAUTIONS AND LIMITATIONS BEFORE OPERATION ................................................ 20
6 ROUTINE OPERATION................................................................................................................. 21
6.1 OPERATIONAL CHECK LIST ......................................................................................................... 21
6.2 SWITCHING ON .......................................................................................................................... 21
6.3 HOW TO INSERT THE PRIMING VESSEL AND THE DISPENSING CASSETTE ........................................ 22
6.4 HOW TO INSERT AND ADJUST THE PLATE ADAPTER ...................................................................... 25
6.5 HOW TO USE THE CONTROL PANEL ............................................................................................. 30
6.5.1 Control panel indicators................................................................................................... 30
6.5.2 Control panel thumbwheels (digital switches) ................................................................. 30
6.5.3 Control panel keys........................................................................................................... 31
6.6 HOW TO SELECT THE 96 / 384-WELL PLATE................................................................................. 31
6.7 HOW TO PRIME.......................................................................................................................... 32
6.8 DISPENSING.............................................................................................................................. 32
6.8.1 How to dispense automatically........................................................................................ 32
6.8.2 How to dispense manually into the specified column(s) ................................................. 33
6.8.3 How to dispense multiple volumes .................................................................................. 33
6.8.3.1 Dispensing tenfold and hundredfold volumes............................................................................. 33
6.8.4 After use .......................................................................................................................... 34
6.9 SHUTDOWN............................................................................................................................... 35
6.10 GUIDELINES FOR USE............................................................................................................. 35
7 MAINTENANCE............................................................................................................................. 36
7.1 REGULAR AND PREVENTIVE MAINTENANCE.................................................................................. 36
7.1.1 General ............................................................................................................................ 36
7.1.2 Immediate ........................................................................................................................ 36
7.2 HOW TO WASH THE DISPENSING CASSETTE ................................................................................. 37
7.3 HOW TO AUTOCLAVE THE DISPENSING CASSETTE ........................................................................ 37
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Thermo Scientific Multidrop
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384 User Manual; Rev. 3.4, Cat. no. 1507010 4
7.4
HOW TO REPLACE THE FUSES .................................................................................................... 38
7.5 HOW TO RECALIBRATE THE DISPENSING CASSETTE...................................................................... 40
7.5.1 Volumetric calibration ...................................................................................................... 40
7.5.2 Gravimetric calibration..................................................................................................... 42
7.6 HOW TO CHANGE THE TUBING SET.............................................................................................. 45
7.6.1 Changing the tip band ..................................................................................................... 45
7.6.2 Fitting the new tubing set step by step ............................................................................ 46
7.7 DISPOSAL OF MATERIALS ........................................................................................................... 57
7.8 DECONTAMINATION PROCEDURE ................................................................................................ 57
7.9 HOW TO PACK FOR SERVICE....................................................................................................... 58
7.10 SERVICE CONTRACTS............................................................................................................. 58
7.11 DISPOSAL OF THE INSTRUMENT .............................................................................................. 58
8 TECHNICAL SPECIFICATIONS ................................................................................................... 60
8.1 GENERAL SPECIFICATIONS ......................................................................................................... 60
8.2 SAFETY SPECIFICATIONS............................................................................................................ 61
8.2.1 Live parts ......................................................................................................................... 61
8.3 IN CONFORMITY WITH THE REQUIREMENTS .................................................................................. 62
8.4 REMOTE CONTROL TO MULTIDROP 384 ...................................................................................... 63
8.4.1 Hardware for the computer port ...................................................................................... 63
8.4.2 Multidrop 384 PC interface .............................................................................................. 63
9 TROUBLESHOOTING................................................................................................................... 66
9.1 TROUBLESHOOTING GUIDE......................................................................................................... 66
9.2 FAQS ABOUT THE MULTIDROP 384 ............................................................................................ 67
9.3 HAZARDS.................................................................................................................................. 68
9.3.1 Electrical .......................................................................................................................... 68
9.3.2 Mechanical....................................................................................................................... 68
9.3.3 Environmental.................................................................................................................. 68
9.3.4 Defects and abnormal stresses....................................................................................... 69
9.4 SERVICE REQUEST PROTOCOL ................................................................................................... 69
9.4.1 Decontamination.............................................................................................................. 69
9.4.1.1 Certificate of Decontamination ................................................................................................... 70
10 WARRANTY CERTIFICATE...................................................................................................... 71
10.1 WARRANTY CERTIFICATE ....................................................................................................... 71
11 ORDERING INFORMATION...................................................................................................... 72
11.1 INSTRUMENTS ....................................................................................................................... 72
11.2 DISPENSING CASSETTES ........................................................................................................ 72
11.3 SPARE PARTS FOR DISPENSING CASSETTES ............................................................................ 73
11.4 PRIMING VESSELS (CONSUMABLES) ........................................................................................ 73
11.5 LIST OF ACCESSORIES............................................................................................................ 73
11.6 LIST OF PLATES (CONSUMABLES)............................................................................................ 73
11.7 LIST OF SPARE PARTS ............................................................................................................ 74
11.8 LIST OF RECOMMENDED SPARE PARTS .................................................................................... 74
12 GLOSSARY AND ABBREVIATIONS ....................................................................................... 75
12.1 KEYWORDS FOR WEB PAGES .................................................................................................. 76
12.2 LITERATURE .......................................................................................................................... 76
13 INDEX......................................................................................................................................... 77
14 TABLE OF FIGURES................................................................................................................. 81
APPENDIX 1. THERMO SCIENTIFIC MULTIDROP 384 BRIEF USER’S GUIDE.............................. 83
APPENDIX 2. THERMO SCIENTIFIC MULTIDROP 384 FEEDBACK FORM.................................... 84
APPENDIX 3. ADDRESSES................................................................................................................. 85
Page 7
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 5
Copyright
Copyright © 2008 Thermo Fisher Scientific Corporation. All rights reserved. First edition published in
1987. Printed in Finland. Reproduction of the accompanying user documentation in whole or in part is
prohibited.
Patents
This product is protected by the following patent: US 4995432.
Trademarks
Immulon, Microfluor and Multidrop are registered trademarks of Thermo Fisher Scientific.
Microlite is a trademark of Thermo Fisher Scientific.
All other trademarks and registered trademarks are the property of their respective holders.
Disclaimer
Thermo Fisher Scientific reserves the right to change its products and services at any time to
incorporate technological developments. This manual is subject to change without prior notice as part
of a continuous product development.
Although this manual has been prepared with every precaution to ensure accuracy, Thermo Fisher
Scientific assumes no liability for any errors or omissions, nor for any damages resulting from the
application or use of this information. This manual supersedes all previous editions.
No liability for consequential damages
Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or
inability to use this product.
Contact information
Thermo Fisher Scientific Oy
P.O. Box 100, FI-01621 Vantaa, Finland
Tel. +358-9-329 100, Fax +358-9-3291 0415
www.thermo.com
Page 8
CChhaapptteerr 11:: SSaaffeettyy SSyymmbboollss aanndd MMaarrkkiinnggss
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 6
1 SAFETY SYMBOLS AND MARKINGS
These symbols are intended to draw your attention to essential information and alert you to the
presence of hazards as indicated.
SAFETY SYMBOLS USED IN THE MULTIDROP 384
Power ON
Power OFF
WEEE symbol
This product is required to comply with the European
Union’s Waste Electrical & Electronic Equipment
(WEEE) Directive 2002/96/EC.
WARNING MARKINGS USED IN THE DOCUMENTATION
Caution: risk of electric shock.
Caution: biohazard risk.
Caution: risk of personal injury to the operator or a safety hazard to the
surrounding area.
Caution: risk of damage to the instrument, other equipment or loss of
performance or function in a specific application.
Page 9
CChhaapptteerr 22:: AAbboouutt tthhee UUsseerr MMaannuuaall
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 7
2 ABOUT THE USER MANUAL
This user manual has been written for the end user (for example, laboratory technician) and provides
information on the Thermo Scientific Multidrop 384 microplate dispenser.
This manual contains the
installation and operating instructions for the Multidrop 384 microplate dispenser.
Read the manual in its entirety prior to operating the instrument.
This user manual has been designed to give you the information you need to:
• Review safety precautions
• Install the Multidrop 384
• Carry out dispensing procedures
• Recalibrate the dispensing cassette
• Perform basic maintenance procedures
• Troubleshoot the instrument performance
This user manual also describes features and specifications of the Multidrop 384 hardware and
onboard software.
Chapter 6 ROUTINE OPERATION explains the dispensing principles and procedures.
In Chapter 9 TROUBLESHOOTING you will find a problem-solving guide. The user should be familiar
with the contents of Chapter 7 on maintenance.
For warranty and ordering information refer to Chapters 10 WARRANTY CERTIFICATE and 11
ORDERING INFORMATION.
In an effort to produce useful and appropriate documentation, we appreciate your comments on this
document to your local Thermo Fisher Scientific representative.
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CChhaapptteerr 33:: IInnttrroodduuccttiioonn ttoo tthhee MMuullttiiddrroopp 338844
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 8
3 INTRODUCTION TO THE MULTIDROP 384
There are two models of the Multidrop 384:
•
5840150 Multidrop 384, 220―240 V 50/60 Hz
•
5840157 Multidrop 384, 110―120 V 50/60 Hz
Each Multidrop 384 comes with one dispensing cassette:
•
24072670 Standard tube dispensing cassette (with 40 cm tubing set)
3.1 Intended use
The Multidrop 384 microplate dispenser is intended for professional research use by trained
personnel. The instrument is intended for automated dispensing of up to eight different reagents
simultaneously into microplates and strips in 96 or 384-well plate format. Use for self-testing is
excluded.
3.2 Principle of operation
The Multidrop 384 is an automatic, programmable, eight-channel microplate dispenser. It has a
peristaltic pump for rapid and continuous dispensing of liquids into 96 and 384-well microplates. It can
dispense 1 to 8 different reagents from external liquid reservoirs into different rows and can be used in
most microtitration techniques, for example, in the fields of bacteriology, cell biology, hematology,
immunology, toxicology and virology. It is particularly useful in the pharmaceutical industry. The
Multidrop 384 is lightweight, transportable and compact on a laboratory bench. It can dispense 20 µl
into the entire 384-well microplate in 20 seconds or 20 µl into the entire 96-well microplate in
5 seconds.
Fig. 3.1 Multidrop 384 microplate dispenser
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CChhaapptteerr 33:: IInnttrroodduuccttiioonn ttoo tthhee MMuullttiiddrroopp 338844
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 9
3.3 Advantages of using the Multidrop 384
The Multidrop 384 microplate dispenser provides several advantages relating mainly to the principle of
operation in that it has:
• Superior accuracy and precision
• A flexible volume range
• High-speed continuous dispensing
• Robot compatibility and versatile remote control commands
• An autoclavable, detachable and calibrateable dispensing cassette
The Multidrop 384 is an automated microplate dispenser designed for high-throughput screening and
microvolume dispensing into 384 and 96-well plates.
The Multidrop 384 can dispense up to eight different reagents with high accuracy, precision and
speed. It fills a 384-well plate with 20 µl/well in 20 seconds or a 96-well plate in 5 seconds. With a
volume range of 5 ― 100 µl for 384-well plates and 5 ― 395 µl for 96-well plates, the Multidrop 384
offers superior flexibility for a wide range of applications.
All reagent lines can be backflushed to the reagent bottle, minimizing the loss of expensive reagents.
From 1 to 24 columns can be selected for 384-well plates and from 1 to 12 columns for 96-well plates.
To ensure sterility and avoid cross contamination, the dispensing cassette is detachable and
autoclavable.
The Multidrop 384 can be used as a standalone instrument or added to robotic systems via an RS-232
serial port.
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CChhaapptteerr 44:: FFuunnccttiioonnaall DDeessccrriippttiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 10
4 FUNCTIONAL DESCRIPTION
4.1 Instrument layout
4.1.1 Front view
1 Instrument cover
2 Control panel
3 Thumbwheels for selecting the volume (
volume µl) and number of columns (col)
4 Plate switch (
plate) for selecting the 96/384-well plate (= rocker switch)
5 Plate carrier
6 Plate adapter
7 Plate adapter lock
8 Transfer rails
9 Priming vessel
10 Lower part slots
11 Upper part slots
12 Rest position slots of the upper part
13 Pump rotor
14 Rotor needles
15 Rotor cover not
pulled over the rotor
16 Pump body
Fig. 4.1 Multidrop 384 front view (A)
15
14
13
8
6
5
1
3
2
4
16
7
12 11
10
9
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CChhaapptteerr 44:: FFuunnccttiioonnaall DDeessccrriippttiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 11
1 Mains switch (ON/OFF)
2 Instrument cover retaining screws
3 Cooling-air outlet
Fig. 4.2 Multidrop 384 front view (B)
1 Rotor cover pulled over the rotor
2 Standard tube dispensing cassette attached
3 Plate adapter
4 Plate carrier
Fig. 4.3 Multidrop 384 front view (C)
2
3
1
2
2
3
4
1
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CChhaapptteerr 44:: FFuunnccttiioonnaall DDeessccrriippttiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 12
1 Tips (8)
2 Lower part of dispensing cassette with tips
3 Tension limiting wires (2)
4 Upper part of dispensing cassette with tubes
5 Sight strips (8)
6 Tubes (A ― H)
7 Tubing weight, eight tubes
8 Calibration screw cover
Fig. 4.4 Close-up of the Multidrop 384 standard tube dispensing cassette
1
7
3
6
8
2
5
4
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CChhaapptteerr 44:: FFuunnccttiioonnaall DDeessccrriippttiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 13
4.1.2 Back view
1 Mains input socket
2 Fuses in the fuse holder cap
3 RS-232C serial interface connector
Fig. 4.5 Multidrop 384 rear view
2
1
3
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CChhaapptteerr 44:: FFuunnccttiioonnaall DDeessccrriippttiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 14
4.2 Multidrop 384 microplate dispenser
The Multidrop 384 microplate dispenser (Fig. 4.6) automates dispensing into microplates. The
microprocessor-based system permits volumes between 5 ― 100 µl for 384-well plates and 5 ― 395 µl
for 96-well plates to be selected (in 5 µl increments) and then dispensed simultaneously into eight
wells (one column of a 96-well microplate). Setting the volume to be dispensed and the number of
columns per plate (maximum 12 columns in 96-well plates and 24 columns in 384-well plates) is
carried out using thumbwheels.
The movement of the microplate is automatic:
In the 96-well plate mode, the columns with 8 wells are filled starting from column 1 up to the column
number set, after which the plate carrier will return to the home position. In the 384-well plate mode,
the columns with 16 wells must be filled in two phases: first every other row (A, C, ..., M, O) is filled
starting from column 1 up to the set column number. Then the plate carrier shifts sideways so that the
remaining rows (B, D, ..., N, P) are filled in the opposite order, from the set column number back to
column 1.
Normally, the microplate moves through the instrument automatically, but a semiautomatic mode is
possible by using the
step and drop keys, which control dispensing into any column of the plate.
The dispensing cassette contains eight individual tubes. The dispensing cassette is detachable. Each
reagent can have a separate dispensing cassette. Alternatively, the same dispensing cassette can be
used with several reagents using the backflush feature. Refer to section 11.2 Dispensing cassettes.
The latest technology has been used in the manufacture of the dispensing cassette which includes an
injection molded manifold. All dispensing cassettes can be autoclaved and Thermo Fisher Scientific
guarantees them up to 50 autoclavings. After this amount it is recommended you replace the cassette.
To avoid damaging the tubes, follow the dispensing cassette maintenance, washing, autoclaving and
storage instructions carefully (see the instructions in sections 6.8.4 After use, 7.2 How to wash the
dispensing cassette and 7.3 How to autoclave the dispensing cassette).
Fig. 4.6 Multidrop 384 microplate dispenser
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CChhaapptteerr 55:: IInnssttaallllaattiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 15
5 INSTALLATION
5.1 Installation check list
The chapter on installation will contain an outline of the points mentioned in the check list below.
Tick Item
T
Unpack the Multidrop 384 instrument carefully. Refer to 5.2.1. Keep the original packaging
and packing material for future transportation.
T
Check the delivery for completeness. Refer to 5.2.2.
T
Check for damage during transport. Refer to 5.2.3.
T
Place the dispenser on a normal laboratory bench taking into account both the environmental
and technical prerequisites. Refer to 5.2.4 and 5.2.6. Leave sufficient clearance on both sides
and at the rear of the unit.
T
Install the instrument. Refer to 5.3.1.
T
Connect the mains supply cable to the mains input socket. See 5.3.2.
T
Carry out the operational check. Refer to 5.4.
5.2 What to do upon delivery
5.2.1 How to unpack
Move the unpacked instrument to its site of operation. Unpack the Multidrop 384 instrument and
accessories carefully with the arrows on the transport package pointing upwards. The following notes
and instructions are sent with the instrument and are immediately available when you open the
package:
• Warranty Certificate card
• packing instructions/packing list
• Multidrop 384 User Manual
Caution: Do not touch or loosen any screws or parts other than those specially
designated in the instructions. Doing so might cause misalignment and will invalidate the
instrument warranty.
Retain the original packaging and packing material for future transportation. The packaging is
designed to assure safe transport and minimize transit damage. Use of alternative packaging
materials may invalidate the warranty. Also retain all instrument-related documentation provided by
the manufacturer for future use.
5.2.2 Checking delivery for completeness
Check the enclosed packing list against order. If any parts are missing, contact Thermo Fisher
Scientific Oy or your local Thermo Fisher Scientific representative.
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CChhaapptteerr 55:: IInnssttaallllaattiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 16
5.2.3 Checking for damage during transport
Visually inspect the transport package, the instrument and the accessories for any possible transport
damage.
If the carton has been damaged in transit, it is particularly important that you retain it for inspection by
the carrier in case there has also been damage to the instrument.
Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit,
but the manufacturer will make every effort to help obtain restitution from the carrier. Upon receipt of
the carrier's inspection report, arrangements will be made for repair or replacement.
Visually check all interconnections in the basic instrument. Check that there are no loose parts inside
the instrument.
If any parts are damaged, contact Thermo Fisher Scientific Oy or your local Thermo Fisher Scientific
representative.
5.2.4 Environmental requirements
When you set up your Multidrop 384, avoid sites of operation with excess dust, vibrations, strong
magnetic fields, direct sunlight, draft, excessive moisture or large temperature fluctuations.
• Make sure the working area is flat, dry, clean and vibration-proof and leave additional room for
accessories, cables, reagent bottles, etc.
• Make sure the ambient air is clean and free of corrosive vapors, smoke and dust.
• Make sure the ambient temperature range is between +10°C (50°F) and +40°C (104°F).
• Make sure relative humidity is between 10% and 80% (non-condensing).
Leave sufficient space (at least 10 cm) on both sides and at the back of the unit to allow adequate air
circulation.
The Multidrop 384 does not produce operating noise at a level that would be harmful. No sound level
measurements are required after installation.
Warning: Do not operate the instrument in an environment where potentially damaging
liquids or gases are present.
5.2.5 Things to avoid
Do not smoke, eat or drink while using the Multidrop 384. Wash your hands thoroughly after handling
test fluids. Observe normal laboratory procedures for handling potentially dangerous samples. Use
proper protective clothing. Use disposable gloves. Be sure the working area is well-ventilated.
Never spill fluids in or on the equipment.
5.2.6 Technical prerequisites
Place the instrument on a normal laboratory bench. The net weight of the entire equipment is approx.
6.2 kg [13.7 lbs.] and the dispensing cassette 124 g [0.3 lb.].
The instrument operates at voltages of 100 – 240 Vac and a frequency range of 50 – 60 Hz.
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CChhaapptteerr 55:: IInnssttaallllaattiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 17
5.3 Setups before you put the instrument into operation
5.3.1 Installation procedure
The following procedure may be carried out by any properly skilled person using standard tools. In
case the local voltage does not correspond to the label at the rear of the instrument on top of the
mains input socket (Fig. 4.5), follow the next installation procedures.
Warning: Ensure the mains switch (Fig. 4.2) on the rear of the left side panel is in the
OFF position. Also ensure the mains supply cable is disconnected (Fig. 5.3).
1. Remove the instrument cover retaining screws (Fig. 5.1 and Fig. 4.2) and lift up the cover.
Fig. 5.1 Location of the cover retaining screws
2. Check that the voltage selector switch (Fig. 5.2) is in the correct position, that is, the voltage
selected corresponds to the local voltage.
Fig. 5.2 Location of the voltage selector
Cover retaining screw on both sides
Voltage
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CChhaapptteerr 55:: IInnssttaallllaattiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 18
3. Visually check that you did not leave any loose parts inside the instrument.
4. Close the cover and fasten the cover retaining screws.
5.3.2 How to ensure startup
1. Warning: Ensure the mains switch (Fig. 4.2) on the rear of the left side panel is in the
OFF position.
2. Connect the mains supply cable to the mains input socket (Fig. 4.3 and Fig. 5.3) at the
bottom of the back panel. If you need to use any other type of mains supply cable than
supplied, use only cables certified by the local authorities.
3. Connect the instrument to a correctly installed line power outlet that has a protective
conductor that is grounded.
Warning: Never operate your instrument from a power outlet that has no ground
connection. Never use a mains supply cable other than the Thermo Scientific mains
supply cable designed for your region.
Fig. 5.3 Connecting the mains supply cable
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CChhaapptteerr 55:: IInnssttaallllaattiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 19
5.4 Operational check
The following procedure should be completed without the dispensing cassette to confirm the correct
functioning of the dispenser prior to normal use.
Connect the mains supply cable (Fig. 5.3).
Switch the instrument on using the mains switch (Fig. 4.2).
• If the instrument starts:
- The green
power LED will light up
- The plate carrier will return to the home position
• If the instrument does not start:
)Pull the rotor cover over the rotor
)Check the mains cable connection
Press
prime/drop to prime
- The pump rotor will rotate
• If the pump rotor does not rotate:
)Pull the rotor cover over the rotor
• If the red
error LED lights up:
)Insert the priming vessel, press the stop key and prime again
Press start
- Starts the dispensing cycle
• If the dispensing cycle does not start:
)Pull the rotor cover over the rotor
• If the red error LED lights up:
)Press the stop key, then press the prime key and finally press the start key again
)Insert the priming vessel, press the stop key, then press the prime key and finally press the
start key again
plate (plate switch)
- Dispenses liquid into the 384 or 96-well microplates
- Try the alternative plate formats by pressing the
start key
step
- Advances the microplate by one column
• If the microplate does not advance:
)Pull the rotor cover over the rotor
After
step press prime/drop
- To dispense a preselected volume into the column selected by steps
- The plate carrier will return to the home position after reaching the last column
After
step press start (partial filling of the tubing)
- To dispense the selected volume automatically from the set column to the last column
empty
- The pump rotor will rotate
• If the pump rotor does not rotate:
)Pull the rotor cover over the rotor
stop
- Terminates the operation and returns the plate to the home position
For more information on the control panel, refer to section 6.5 How to use the control panel.
1
2
8
7
5
4
3
6
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384 User Manual; Rev. 3.4, Cat. no. 1507010 20
5.5 Operating precautions and limitations before operation
1. Read this manual in its entirety, as it contains information necessary to ensure safe operation.
2. Always ensure that the local supply voltage in the laboratory conforms to that specified on the type
label on the back of the instrument.
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CChhaapptteerr 66:: RRoouuttiinnee OOppeerraattiioonn
Thermo Scientific Multidrop
®
384 User Manual; Rev. 3.4, Cat. no. 1507010 21
6 ROUTINE OPERATION
6.1 Operational check list
The chapter on routine operation will contain an outline of the points mentioned in the check list below.
Tick Item
T
Switch on the instrument. Refer to 6.2.
T
Insert the priming vessel and the dispensing cassette. Refer to 6.3.
T
Use the plate adapter for the plate carrier to function. Refer to 6.4.
T
Pull the rotor cover over the rotor. Refer to 6.3.
T
Familiarize yourself with the control panel. Refer to 6.5.
T
Press the plate switch (
plate) to position 96 or 384 according to the plate type you use.
Refer to 6.5.2.
T
Prime all the eight channels so that there are no air bubbles in the tubes. Refer to 6.7.
T
Carry out dispensing either manually or automatically. Refer to 6.8.
T
After all the plates have been dispensed, press the
empty key to return the reagent or liquid
from the tubes to the reservoir. Refer to 6.8.4.
T
Wash the dispensing cassette by priming it with deionized distilled water when not in use.
Refer to 6.8.4 and 7.2.
T
After washing, store the dispensing cassette in the rest position. Refer to 6.8.4 and 7.2.
T
Maintain the instrument regularly. Refer to Chapter 7.
Note: Keep the dispensing cassette in the rest position when the dispensing cassette is
not in use.
6.2 Switching on
Switch on the instrument by using the mains switch on the left-hand side of the instrument (Fig. 4.2).
The green
power LED will light up.
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6.3 How to insert the priming vessel and the dispensing cassette
1. Ensure that the priming vessel is inserted correctly into its slot on the left of the plate carrier
(Fig. 6.1). You have fastened it correctly when it snaps slightly and is inserted into the sensor
slot.
Fig. 6.1 Inserting the priming vessel into place
2. Take the lower part of the cassette into your right hand with the dispensing tips pointing down
and the upper part into your left hand (Fig. 6.2).
Fig. 6.2 Inserting the standard tube dispensing cassette
Priming vessel
sensor slot
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®
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3. Carefully place the eight tubes below the pump rotor and insert the lower part of the cassette into
the lower part slots of the pump body (Fig. 6.3). Check that the tubes are freely placed below the
rotor and the tension limiting wires below the rotor shaft.
Fig. 6.3 Inserting the lower part of the dispensing cassette into its slots
4. Take a firm grip on the upper part of the cassette with your right hand and carefully pull the tubes
around the rotor until the upper part reaches and fits into the upper part slots of the pump body
(Fig. 6.4 and Fig. 4.1).
Fig. 6.4 Inserting the upper part of the dispensing cassette
Lower part slots
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Note: The tension limiting wires are designed to prevent the user from pulling the tubes
too much as excessive tension may damage the tubes or change the calibration of the
cassette.
5. Double-check that both the upper and the lower parts are properly placed into their
corresponding slots (Fig. 6.3 and Fig. 6.5).
Fig. 6.5 Lower and upper parts of the dispensing cassette inserted evenly into their slots
6. Ensure that all the tubes are evenly placed on the rotor needles, four tubes on each half of the
rotor.
7. Ensure also that the tension limiting wires have a loose fit around the rotor shaft.
8. PULL THE ROTOR COVER OVER THE ROTOR (Fig. 6.6). The plate carrier will then return to
the furthest right of the instrument, that is, the home position. The microswitch controls that the
cover is properly placed over the rotor. The Multidrop 384 is now ready for priming.
Fig. 6.6 Pulling the rotor cover over the rotor
Upper part slots
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Note: If the rotor cover is not pulled over the rotor, the instrument will not function.
Note: It is recommended you change the dispensing cassette or tubing set after
dispensing about 3000 – 5000 96-well plates, depending on the dispensing frequency and
the liquids used.
Note: Keep the dispensing cassette in the rest position when the dispensing cassette is
not in use.
6.4 How to insert and adjust the plate adapter
The Multidrop 384 requires a plate adapter for the plate carrier to function. Two different plate
adapters are included (Fig. 6.7).
Fig. 6.7 Two different plate adapters
The PLATE ADAPTER, PLATE
HEIGHT MAX. 15 MM, is
suitable for plates approximately
15 mm high, for example, 96well plates and some 384-well
plates.
The PLATE ADAPTER, PLATE
HEIGHT MAX. 11 MM, is
suitable for lower plates, for
example, 384-well plates.
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The plate adapter is inserted into the plate carrier in the following way:
1. Open the plate adapter lock located in the front left corner of the plate carrier (Fig. 6.8). Select
the plate adapter according to the height of the plate.
Fig. 6.8 Opening the plate adapter lock
2. Take the plate adapter in your left hand and lower the right end. Place the plate adapter into the
right end corner of the plate carrier (Fig. 6.9).
Fig. 6.9 Placing the right end of the plate adapter
Plate adapter lock
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3. Then press the left end of the plate adapter onto the plate carrier (Fig. 6.10).
Fig. 6.10 Pressing the left end of the plate adapter
4. Lock the plate adapter into place with the plate adapter lock located in the front left corner of the
plate carrier (Fig. 6.11).
Fig. 6.11 Closing the plate adapter lock
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5. To adjust the plate size, loosen first the two attached plastic-covered screws on the right of the
plate carrier (Fig. 6.12).
Fig. 6.12 Loosening the screws on the right of the plate carrier
6. Then loosen the two attached plastic-covered screws at the front of the plate adapter (Fig. 6.13).
Fig. 6.13 Loosening the screws at the front of the plate adapter
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7. Insert the microplate onto the plate adapter. Push the microplate at the same time towards the
left-hand top corner of the plate adapter while you fasten the front screws (Fig. 6.14).
Fig. 6.14 Fastening the front screws of the plate adapter
8. Then fasten the screws to the right of the plate carrier, pushing the microplate simultaneously
towards the left-hand corner of the plate adapter (Fig. 6.15). If the instrument is used for robotics,
leave sufficient moving space for the microplate according to the robotic specifications.
Fig. 6.15 Fastening the screws to the right of the plate carrier
Note: Ensure the dispensing cassette is attached and the rotor cover has been pulled
over the rotor.
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Thermo Scientific Multidrop
®
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6.5 How to use the control panel
The keypad has an embossed, tactile membrane with five keys. There are two control panel indicators
(LEDs) in all.
The control panel is shown in Fig. 6.16.
Fig. 6.16 Multidrop 384 control panel
6.5.1 Control panel indicators
power
The green
power LED indicates that the dispenser is powered.
error The red error LED shows when the dispenser has some kind of error.
6.5.2 Control panel thumbwheels (digital switches)
plate
96/384-well switch: sets the plate carrier movement and dispensing for 96 or 384well plates.
volume µl
Reagent volume thumbwheels: sets the volume to be dispensed per well.
col
Column thumbwheels: sets the number of columns per plate, up to 12 with 96well plates or up to 24 with 384-well plates.
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6.5.3 Control panel keys
start
Starts the dispensing cycle.
prime/
drop
Primes the cassette if the tips are above the priming vessel.
Primes continuously as long as the user presses the key.
Dispenses the adjusted volume if the tips are above the wells.
empty
Empties the liquid from the tubes to the liquid reservoir.
step
Advances the microplate by one column.
stop
Terminates the operation and returns the plate to the home position.
6.6 How to select the 96 / 384-well plate
• Press the plate switch (
plate) down into position 96 or 384 according to the plate type to be used
(Fig. 6.17).
• Select the number of columns (
col) between 1 and 24 in the 384 mode.
• Select the number of columns (
col) between 1 and 12 in the 96 mode (Fig. 6.17). Note that if you
set the column selection above 12 in the 96 mode, the red
error light will light up and the
dispensing operation will be terminated.
Fig. 6.17 Multidrop 384 control panel
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6.7 How to prime
Note: Ensure that the instrument is sufficiently level on the table to prevent liquids from
overspilling when priming.
Note: Ensure that the liquid temperature is close to the calibration temperature of 22°C.
Differences greater than ± 2°C will cause an error in the results.
Note: Silicon tubing (silicon rubber) is resistant to a number of chemical liquids. Most
water solutions typically used in a medical laboratory can be dispensed with the Multidrop
384. Some strong substances may, however, harm the silicone tubing and cause swelling
or breaking. If the substance has even a slight effect on silicone, the calibration of the
cassette may be affected and the cassette may become unusable.
1. Place the eight tube ends into the same liquid reservoir or into different reservoirs taking care
that all tube ends are below the liquid level. Use the tubing weight provided if possible.
2. Check that the priming vessel is firmly inserted next to the plate carrier. The Multidrop 384 will
not operate if the priming vessel is not completely inserted into its slot.
3. Press the
prime/drop key until all eight channels are dispensing continuously into the priming
vessel and there are no more air bubbles in the tubes. It is recommended you fill the priming
vessel two or three times with the reagent to be used before starting the actual dispensing in
order to bring the tubes into working condition.
Note: If you press the
prime/drop key at the same time as the rotor cover is pulled over
the rotor, the program enters into the volumetric calibration mode.
Note: You can empty the contents of the priming vessel back into the reagent reservoir by
pulling the priming vessel out of its slot if necessary. It is recommended you do this if you
use expensive reagents. Therefore, wash the priming vessel always after each use to
avoid any cross contamination. If you use several liquids at the same time in different rows,
you can order a special eight-cavity priming vessel for this purpose to prevent mixing the
liquids. Also a self-draining (flow-through) priming vessel is available. Refer to section 11.4
Priming vessels (consumables).
6.8 Dispensing
6.8.1 How to dispense automatically
1. Set the required reagent volume per well. The permitted volume range is 5 ― 100 µl for 384-well
plates and 5 ― 395 µl for 96-well plates.
2. Set the required number of columns to be dispensed per plate.
3. Place the microplate onto the plate carrier equipped with a suitable plate adapter. Refer to
section 6.4 How to insert and adjust the plate adapter.
4. Press the
prime/drop key to prime the tubes. Refer to section 6.7 How to prime.
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5. Press the
start key. The Multidrop 384 will now automatically dispense the preselected volume
of reagent into the preselected number of columns. When dispensing has been completed, the
plate carrier will automatically return to its home position.
6. Now you can dispense any number of plates by only changing the plate and pressing
start.
Note: Check that there is always enough liquid in the reservoir and ensure that all the
tube ends are below the liquid level. Use the tubing weight supplied.
Note: Check after each dispensing that the priming vessel has not filled up. Empty it if
necessary, otherwise the liquids may spill over during operation. Note that the Multidrop
384 always primes itself before each plate filling to ensure maximum accuracy and
precision. It is recommended you empty the priming vessel after dispensing 30 – 40 plates.
Alternatively, use the self-draining priming vessel.
6.8.2 How to dispense manually into the specified column(s)
1. Set the desired reagent volume. The permitted volume range is 5 ― 100 µl in the 384-well plate
mode and 5 ― 395 µl in the 96-well plate mode.
2. Place the microplate onto the plate carrier equipped with a suitable plate adapter.
3. Use the
step key to move the plate carrier column by column until you reach the desired
column.
4. Press the
prime/drop key. The Multidrop 384 will now dispense the preselected volume into
the column desired. The plate carrier will return to the home position after reaching the last
column. If you use the
start key, the instrument will automatically dispense the selected volume
from the set position to the last column selected.
Note: If you have pressed either the
stop or empty key, the Multidrop 384 refuses to
start dispensing before you have pressed the
prime/drop key once again.
6.8.3 How to dispense multiple volumes
Ten- or hundredfold volumes can be dispensed compared to the volume chosen.
6.8.3.1 Dispensing tenfold and hundredfold volumes
1. Press the
stop and prime/drop keys simultaneously.
2. Having done that first, release the
stop key and then the prime/drop key. Note that the
Multidrop 384 is in the volumetric calibration mode.
3. Now you can push the plate carrier into the desired dispensing position.
4. Set one tenth of the volume required on the thumbwheel. Then press the
start key and a tenfold
volume will be dispensed.
5. Set one hundredth of the volume required on the thumbwheel. Then press the
step key and a
hundredfold volume will be dispensed.
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6.8.4 After use
After all the plates have been dispensed, press the
empty key to return the reagent or liquid from the
tubes to the reservoir. This saves expensive reagents.
If there is only a short break in dispensing, no washing or cleaning of the tubes is necessary.
However, after pressing the
empty key, release the tubing tension by pulling the upper part of the
cassette from its slots and place it into its rest position slots located on the left-hand side of the rotor
(Fig. 6.18).
If there is a longer break (for example, a day), wash the dispensing cassette by priming it with
deionized distilled water or with special washing detergent if necessary. In the end, rinse the washing
detergent away with deionized distilled water.
After washing, store the dispensing cassette in the rest position (Fig. 6.18).
Note: Keep the dispensing cassette in the rest position when the dispensing cassette is
not in use.
Fig. 6.18 Rest position of the dispensing cassette
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6.9 Shutdown
Remove any microplates still on the plate carrier. Dispose of all microplates and strips as
biohazardous waste.
After all the plates have been dispensed, press the
empty key to return the reagent or liquid from the
tubes to the reservoir. This saves expensive reagents.
If there is only a short break in dispensing, no washing or cleaning of the tubes is necessary.
However, after pressing the
empty key, release the tubing tension by pulling the upper part of the
cassette from its slots and place it into its rest position slots located on the left-hand side of the rotor
(Fig. 6.18).
If there is a longer break (for example, a day), wash the dispensing cassette by priming it with
deionized distilled water or with special washing detergent if necessary.
After washing, store the dispensing cassette in the rest position (Fig. 6.18).
Switch the Multidrop 384 off by pressing the mains switch (Fig. 4.2) at the end of the left side panel of
the instrument into the OFF position.
Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled water,
a mild detergent (SDS, sodium dodecyl sulfate) or soap solution.
If you have spilt infectious agents on the dispenser, disinfect with 70% alcohol or some other
disinfectant (see section 7.8 Decontamination procedure).
6.10 Guidelines for use
• When the
error LED is on, the start or step keys will not function.
• To clear the error and continue the dispensing procedure, press the
stop key and wait until the
plate carrier has returned to the home position. The dispensing procedure can then be restarted.
• If you press the
stop key, all operations will stop. The plate carrier is automatically returned to
the home position. The Multidrop 384 will now require priming again.
• Preventative maintenance should be followed closely to keep the microplate dispenser in the best
condition for maximum reliability. A poorly maintained instrument will not give the best results.
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7 MAINTENANCE
7.1 Regular and preventive maintenance
7.1.1 General
Routine and service procedures must be performed by the user to prevent unnecessary wear or
hazards and are described below at the frequency with which they should be applied.
Abrasive cleaning agents are not recommended, because they are likely to damage the paint finish.
It is recommended you clean the case of the instrument periodically to maintain its good appearance.
A soft cloth dampened in a warm, mild detergent solution will be sufficient.
Clean the outside of the instrument and the plate carrier with clean low-pressure compressed air or a
cloth dampened with water or a mild detergent when necessary.
Painted surfaces can be cleaned with most laboratory detergents. Dilute the cleaning
agent as recommended by the manufacturer. Do not expose painted surfaces to
concentrated acids or alcohols for prolonged periods of time as damage may occur.
Clean the control panel with a mild laboratory detergent.
Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol.
If any surfaces have been contaminated with biohazardous material, a mild sterilizing
solution should be used.
The dispensing cassette is autoclavable. Do not autoclave any other part of this
instrument.
7.1.2 Immediate
Although the Multidrop 384 is constructed from high-quality materials, you must immediately wipe
away spilled saline solutions, solvents, acids or alkaline solutions from outer surfaces to prevent
damage and wipe with deionized distilled water.
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7.2 How to wash the dispensing cassette
Wash the tubes by priming them first with tap water and then with deionized distilled water. Make sure
that all the tubes are washed properly.
If cleaning with water is insufficient, use a mild laboratory detergent and then prime with large
amounts of tap water followed by deionized distilled water.
The dispensing cassette can be dried at room temperature.
After washing, store the dispensing cassette in the rest position (Fig. 6.18).
If the instrument is not used for a couple of hours, insert the dispensing cassette into the rest position.
Refer to section 6.8.4 After use. Handle the dispensing cassette with great care so that you do not
damage the tubes and dispensing tips. These factors will affect the service life of the dispensing
cassette.
7.3 How to autoclave the dispensing cassette
The standard tube dispensing cassette (Cat. no. 24072670) can be autoclaved 50 times with the same
tubes.
The autoclaving conditions of the dispensing cassette are as follows: 1 bar pressure at 121°C for
20 minutes.
Note: After autoclaving, the dispensing cassette must cool down to room temperature
before use.
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7.4 How to replace the fuses
1. Switch off (Fig. 4.2) and unplug the instrument from the mains input socket (Fig. 4.5).
2. Remove the fuse holder cap with a screwdriver or similar tool. Press both ends of the fuse holder
cap with the tool (Fig. 7.1 and Fig. 7.2).
Fig. 7.1 Removing the fuse holder cap (A)
Fig. 7.2 Removing the fuse holder cap (B)
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3. Check the fuses in the fuse holder cap (Fig. 7.3).
Fig. 7.3 Fuse holder cap with fuses
4. Replace the blown fuse with the same type and push the fuse holder cap back into its position
(Fig. 7.4).
Fig. 7.4 Inserting the fuse holder cap
5. Reconnect to the mains input socket and switch the power on.
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7.5 How to recalibrate the dispensing cassette
The dispensing cassette has been calibrated by the manufacturer with deionized distilled water at
22°C ± 2°C. It is recommended you recalibrate the cassette after dispensing about 1000 96-well
plates or after every three months. It is also recommended you recalibrate the dispensing cassette if
you have forgotten the cassette in the Multidrop 384 under tension for prolonged periods, for example,
a weekend or longer.
Two different calibration methods are described below, that is, volumetric calibration and gravimetric
calibration.
7.5.1 Volumetric calibration
For volumetric calibration, use the following steps:
1. Remove the priming vessel and microplate.
2. Install the dispensing cassette into the Multidrop 384 if it is not already installed. Place the
dispensing cassette into the rest position (Fig. 6.18). Refer to section 6.3 How to insert the priming
vessel and the dispensing cassette.
3. Install the tube of the calibration device into the tip of the channel to be recalibrated (Fig. 7.5), for
example, A.
Fig. 7.5 Placing the tube onto the tip of channel A
4. Insert the dispensing cassette into the working position so that the lower and upper parts of the
dispensing cassette are inserted into their slots (Fig. 6.5).
5. Pull the rotor cover over the rotor.
6. Place the tube of channel A into the reservoir with water at room temperature (working
temperature).
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7. Open the calibration screw cover on the upper part of the cassette (Fig. 7.7 and Fig. 4.4).
8. Set the volume to 100 µl.
9. Press the
stop and prime/drop keys at the same time. After that first release the stop key
and then the
prime/drop key. This brings the Multidrop 384 into the volumetric calibration
mode.
10. Press the
start key as many times until the water enters the calibration pipette.
Note: Take care not to press the
step key accidentally or otherwise 10 ml of water will be
dispensed.
11. Adjust the water level in the pipette to the lower mark by pulling or pushing the syringe piston
(Fig. 7.6).
Fig. 7.6 Adjusting the liquid level first to the lower mark and then to the upper mark
12. Press the
start key and wait until the water level stops. It should be at the upper mark, 1000 µl
(± 1.5%) (Fig. 7.6).
13. If the volume is higher than 1000 µl, turn the calibration screw (Fig. 7.7) clockwise with a
screwdriver; one turn for each 10 µl (1% from the volume dispensed); if the volume is lower than
1000 µl, turn in the opposite direction.
Upper mark
Lower mark
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®
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Fig. 7.7 Screwing the calibration screws according to the calibration results
14. Press the
empty key and adjust the water level in the pipette to the lower mark and repeat Steps
10 to 13 until the result is 1 ml. Three accurate calibrations must be obtained.
15. Change the tube to the next channel and repeat Steps 10 to 14.
16. After all the channels have been recalibrated, press the
stop key to reset the Multidrop 384 into
normal operation and reinstall the priming vessel.
17. Close the calibration screw cover.
7.5.2 Gravimetric calibration
For gravimetric calibration, use the following steps:
1. Install the dispensing cassette into the working position so that the lower and upper parts of the
dispensing cassette are inserted into their slots (Fig. 6.5).
2. Pull the rotor cover over the rotor.
3. Ensure that the priming vessel is installed. Refer to section 6.3 How to insert the priming vessel
and the dispensing cassette.
4. Ensure that you have the plate adapter for 15 mm high plates installed. Refer to section 6.4 How
to insert and adjust the plate adapter.
Calibration screw
cover opened
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Note: Keep the dispensing cassette in the rest position when the dispensing cassette is
not in use.
5. Place the tube of channel A into the reservoir with water at room temperature (working
temperature).
6. Use 8 x 12 well microstrips and adjust the Multidrop 384 for 100 µl in 10 columns and set the
plate switch (
plate) for 96-well plates (Fig. 7.8).
Fig. 7.8 8 x 12 well microstrips adjusted onto the plate adapter
7. Press the
prime/drop key to prime the tubes. Refer to section 6.7 How to prime.
8. Open the calibration screw cover on the upper part of the cassette.
9. Tare an analytical balance (resolution 0.01 mg) with one empty, dry 1 x 12 well microstrip.
10. Place the microstrip into the microplate frame on the row corresponding to the channel to be
recalibrated (from A to H). Press the
start key.
11. Weigh the filled microstrip (Fig. 7.9). The balance should now read 1000 mg ± 20 mg.
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Fig. 7.9 Removing the filled microstrip to be weighed
12. If the balance reads less than 980 mg, turn the calibration screw counterclockwise: one turn for
each 10 mg (1% from the volume dispensed). If the balance reads more than 1020 mg, turn in
the opposite direction (Fig. 7.10). Recheck the delivery after each adjustment using Steps 9 to
11. Three acceptable calibrations must be obtained.
Fig. 7.10 Screwing the calibration screw according to the calibration results
13. Continue in the same way with each channel until all eight channels have been recalibrated.
14. Close the calibration screw cover.
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7.6 How to change the tubing set
7.6.1 Changing the tip band
1. Place the dispensing cassette onto the laboratory bench with the dispensing tips pointing down.
2. Unfasten the four cover retaining screws of the lower part of the dispensing cassette (Fig. 7.11).
Fig. 7.11 Removing the cover plate of the lower part of the dispensing cassette
3. Remove the tip manifold, the tube fasteners and the rest of the tubing from the lower part of the
dispensing cassette. Then detach the tip band from the tubing by pulling the tubes away from the
tip band (Fig. 7.12).
Fig. 7.12 Removing the old tip band
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4. Replace the tip band (Fig. 7.13). The tips of the tip band must not be clogged in any way but be
clean to function correctly (Fig. 7.14).
Fig. 7.13 Tip band removed
Fig. 7.14 New tip band
7.6.2 Fitting the new tubing set step by step
1. First place the new tubing set onto the laboratory bench with the tip band attached (Fig. 7.15).
Ensure that you have saved the eight calibration screws and the tubing weight from the previous
tubing set.
Tip
Insert tube here
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Fig. 7.15 New tubing set with the tip band attached
2. Remove the four cover retaining screws on the lower part of the dispensing cassette, the tubes
with the tube fasteners and the tip manifold with the tips, the three cover retaining screws on the
upper part of the dispensing cassette, the two tension limiting wires, the calibration screws and
the tubes with the tube fasteners. Save the calibration screws and the tubing weight for further
use when installing the new tubing set.
3. Insert the tip manifold so that the tips go through the holes in the lower part of the dispensing
cassette (Fig. 7.16) and guide the tubes through the eight openings in the side wall by pressing
the small cubic tube fasteners into place (Fig. 7.17). Ensure that each tube is in its designated
opening (Fig. 7.18).
Fig. 7.16 Inserting the tip manifold into the lower part of the dispensing cassette
Tubing identification plate
Tube fasteners
Tip band
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Fig. 7.17 Pressing the tube fasteners into place
Fig. 7.18 New tubing inserted into the lower part of the dispensing cassette
4. Attach the tension limiting wires into their slots (Fig. 7.19).
Fig. 7.19 Tension limiting wires attached
Slots for tension limiting wires
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5. Fasten the four cover retaining screws of the lower part of the dispensing cassette (Fig. 7.20).
Fig. 7.20 Replacing the lower part cover of the dispensing cassette
6. Once the lower part of the dispensing cassette has been fitted, place the remaining parts
belonging to the upper part of the dispensing cassette onto the laboratory bench (Fig. 7.21).
Fig. 7.21 Parts belonging to the upper part of the dispensing cassette
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7. First place the plastic sight window with the grooves pointing upwards onto the bottom cover of
the upper part of the dispensing cassette, if it is not already there (Fig. 7.22).
Fig. 7.22 Placing the sight window
8. After that press the white tubing identification plate into place on the right-hand side of the bottom
cover of the upper part of the dispensing cassette (Fig. 7.23).
Fig. 7.23 Pressing the tubing identification plate into place
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9. Then press each tube fastener into place in its groove on the plastic sight window (Fig. 7.24).
Fig. 7.24 Pressing the tube fasteners into place
10. Fasten the calibration screws from the previous tubing into their places using a hexagonal
screwdriver (Fig. 7.25).
Fig. 7.25 Fastening the calibration screws
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11. Insert the tension limiting wires into their slots into the upper part of the dispensing cassette
(Fig. 7.26).
Fig. 7.26 Calibration screws fastened
12. Replace the upper part cover of the dispensing cassette carefully (Fig. 7.27).
Fig. 7.27 Replacing the cover of the upper part of the dispensing cassette
13. Ensure you do not get the tubing trapped or bent between the bottom and upper cover of the
upper part of the dispensing cassette (Fig. 7.28).
Fig. 7.28 Ensuring the tubing does not get trapped
Slots for tension limiting wires
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14. Fasten the three cover retaining screws of the upper part of the dispensing cassette, leaving the
front screw slightly open (Fig. 7.29).
Fig. 7.29 Fastening the cover retaining screws
15. Insert the calibration screw cover easier by lifting or bending the upper cover slightly (Fig. 7.30).
Fig. 7.30 Inserting the plate on the calibration screws
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16. Fasten the front cover retaining screw (Fig. 7.31). Continued below.
Fig. 7.31 Lower and upper part of the dispensing cassette fitted
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17. Turn the dispensing cassette so that the eight sight strips are visible (Fig. 7.32). Look at the eight
sight strips in the upper part of the dispensing cassette. You will notice that the tubes are not
precalibrated. First open the calibration screw cover. For precalibration of the dispensing
cassette, adjust the tube fasteners in the sight strips roughly to the middle using a hexagonal
screwdriver (Fig. 7.33).
Fig. 7.32 Dispensing cassette before precalibration
Fig. 7.33 Precalibrating the dispensing cassette
Sight
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18. When the tube fasteners have all been adjusted roughly to the middle of the sight strip, close the
calibration screw cover (Fig. 7.34).
Fig. 7.34 Dispensing cassette precalibrated
19. Insert the eight tubes into the tubing weight either at this stage or earlier (Fig. 7.35). The new
tubing set has now been successfully fitted.
Fig. 7.35 Inserting the tubes into the tubing weight
20. Calibrate the dispensing cassette according to the instructions in section 7.5 How to recalibrate
the dispensing cassette.
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7.7 Disposal of materials
Refer to local regulations for the disposal of infectious material.
The samples can be potentially infectious. Dispose of all used microplates, microstrips,
disposable gloves, syringes, disposable tips, etc. as biohazardous waste.
7.8 Decontamination procedure
If there is any risk of contamination with biohazardous material, the procedure recommended below or
some other corresponding efficient decontamination procedure must be performed.
You must perform the complete decontamination procedure before shipping the instrument to Thermo
Fisher Scientific Oy, for example, for repair, or relocating the instrument from one laboratory to
another. Refer to section 7.9 How to pack for service. Decontamination is not required for the proper
functioning of the instrument.
Examples of decontaminants
• Ethanol 70%
• Virkon solution 1 ― 3%
• Glutaraldehyde solution 4%
• Chloramine T
• Microcide SQ 1:64
IF LOCAL OR LABORATORY REGULATIONS PRESCRIBE REGULAR DISINFECTION,
IT IS NOT ADVISABLE TO USE FORMALDEHYDE, SINCE EVEN SMALL TRACES OF
FORMALDEHYDE AFFECT THE ENZYME BEING USED IN EIA TESTS IN A NEGATIVE
WAY RESULTING IN BAD TEST RESULTS.
Always use disposable gloves and protective clothing and operate in a well-ventilated area.
Do not autoclave any other parts of the instrument except the dispensing cassette.
1. Prepare the decontaminant: for example, 1 ― 3% Virkon solution or 200 ml 4% glutaraldehyde
solution (or another agent recommended by your safety officer).
2. Empty the reagent container.
3. Switch the power OFF and disconnect the mains supply cable (Fig. 4.2).
4. Disinfect the outside of the instrument using a wad of cotton wool/cloth soaked in 70% ethanol.
5. Place the plate adapter and dispensing cassette, for example, in 1 ― 3% Virkon solution or
200 ml 4% glutaraldehyde solution for 10 min and rinse them with deionized distilled water.
6. Place the entire instrument in a large plastic bag. Ensure that the reagent containers are
disconnected and open.
7. Place a wad of cotton wool soaked in the prepared solution into the bag. Ensure that the wad
does not make contact with the instrument.
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8. Close the bag firmly and leave the instrument in the bag for at least 24 hours.
9. Remove the instrument from the bag.
10. Clean the instrument using a mild detergent.
11. Remove any stains using 70% ethanol.
12. Flush the fluid path of the instrument with deionized distilled water using the priming procedure.
13. The dispensing cassette must be autoclaved.
14. Also wash and disinfect the reagent containers.
15. After performing this decontamination procedure, enclose a signed and dated Certificate of
Decontamination both inside the transport package and attached to the outside of the package.
7.9 How to pack for service
When you ship the instrument for service remember to:
• Inform about the use of hazardous materials.
• Decontaminate the instrument beforehand.
• Pack the instrument according to the enclosed packing instructions.
• Use the original packaging to ensure that no damage will occur to the instrument during shipping.
Any damage will incur additional labor charges.
• Enclose a dated and signed Certificate of Decontamination (see section 9.4.1.1) both inside and
attached to the outside of the package, in which you return your instrument (or other items).
• Enclose the return authorization number (RGA) given by the Thermo Fisher Scientific
representative.
• Indicate the fault after you have been in touch with your local Thermo Fisher Scientific
representative or the Thermo Fisher Scientific technical service department.
See section 8.1 for details on storage and transportation temperatures.
7.10 Service contracts
It is recommended you maintain and service the instrument regularly every twelve months on a
contract basis by the manufacturer's trained service engineers. This will ensure the product is properly
maintained and gives trouble-free service. Contact the Thermo Fisher Scientific technical service
department for more details.
7.11 Disposal of the instrument
1. Decontaminate the instrument prior to disposal. See sections 7.8, 9.4.1 and 9.4.1.1
on decontamination. Also wash and disinfect the dispensing cassettes prior to
disposal.
2. Follow laboratory and country-specific procedures for biohazardous or radioactive
waste disposal. Dispose of the instrument according to the legislation stipulated by
the local authorities concerning take-back of electronic equipment and waste. The
proposals for the procedures vary by country.
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WEEE symbol Thermo Fisher Scientific has contracted with one or more
recycling/disposal companies in each EU Member State (European Country), and this
product should be disposed of or recycled through them. Further information on
Thermo Fisher Scientific’s compliance with these Directives, the recyclers in your
country, and information on Thermo Scientific products which may assist the
detection of substances subject to the RoHS Directive are available at
www.thermo.com/WEEERoHS
.
3. Regarding the original packaging and packing materials, please use the recycling
operators known to you.
4. For more information, contact your Thermo Fisher Scientific representative.
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8 TECHNICAL SPECIFICATIONS
8.1 General specifications
Overall dimensions
310 mm (12.2 in.) (W) x 320 mm (12.6 in.) (D) x 155 mm (6.1 in.) (H)
Weight (total) Instrument Dispensing cassette
6.2 kg [13.7 lbs.] 124 g [0.3 lb.]
Mains power supply
100 ― 120 Vac, 50/60 Hz, nominal (operating range 90 ― 130 Vac)
200 ― 240 Vac, 50/60 Hz, nominal (operating range 180 ― 256 Vac)
Power consumption
50 VA max.
Heat dissipation
171 BTU max.
Fuses
2 x 300 mA for 200 ― 240 V, UL 198G Time Delay, 5 x 20 mm
2 x 600 mA for 100 ― 120 V, UL 198G Time Delay, 5 x 20 mm
Operating conditions
(indoor use only)
+10°C ― +40°C
Transportation
conditions
-40°C ― +70°C, packed in transport packaging
Storage conditions
-25°C ― +50°C, packed in transport packaging
Autoclaving
conditions of the
dispensing cassette
pressure: 1 bar
temperature: 121°C
time: 20 min
autoclavings: 50 times
Control panel
Keys:
start, prime/drop, empty, step, stop
Selection switches: plate (= 96/384), volume µl, col (= column)
Interface
Serial RS-232C
384-well dispensing 96-well dispensing
Dispensing volume
5 – 100 µl in 5 µl increments
5 – 395 µl in 5 µl increments
Dispensing speed
20 s/20 µl into 384 wells 5 s/20 µl into 96 wells
Dispensing accuracy
± 3% or ± 1 µl, whichever is greater
5 µl: ± 3% or ± 1 µl, whichever is greater
20 µl: ± 2% (typical*)
100 µl: ± 1% (typical*)
Valid when counted for the entire plate
Dispensing precision
5 µl: CV ≤ 10% or SD ≤ 0.6 µl, whichever is greater
10 ― 395 µl: CV ≤ 3.0% or SD ≤ 0.4 µl, whichever is greater
5 µl: ≤ 10% (typical)
20 µl: ≤ 1.5% (typical)
100 µl: ≤ 1% (typical)
Valid when counted for the entire plate
* using the calibration procedure in section 7.5 the accuracy can be improved to ± 1.5%
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8.2 Safety specifications
8.2.1 Live parts
The instrument is safe to operate with the covers fitted and these must not be removed during operation.
The covers protect the user from live parts and they should only be removed after switching the
instrument off and disconnecting the mains supply cable, and only by suitably qualified maintenance
and repair personnel.
Voltages dangerous for human beings are present in this instrument. Before removing any
covers, disconnect the instrument from the power supply.
The Multidrop 384 fulfils the following requirements:
EN 61010-1:1993 + A2:1995/IEC 61010-1:1990 + A1:1992 + A2:1995
taking into account US and CA National differences
EN 61010-1:2001 (Ed. 2)
The safety specifications are also met under the following environmental conditions in addition to or in
excess of those stated in the operating conditions:
Altitude
Up to 2000 m
Temperature
+10°C ― +40°C
Mains supply fluctuations
± 10% from nominal
Installation category (overvoltage category)
II according to IEC 60664-1 (Note 1)
Pollution degree
2 according to IEC 60664-1 (Note 2)
Notes
1. The installation category (overvoltage category) defines the level of transient overvoltage, which
the instrument is designed to withstand safely. It depends on the nature of the electricity supply
and its means of overvoltage protection. For example, in CAT II, which is the category used for
instruments in installations supplied from a supply comparable to public mains, such as hospital
and research laboratories and most industrial laboratories, the expected transient overvoltage is
2500 V for a 230 V supply and 1500 V for a 120 V supply.
2. The pollution degree describes the amount of conductive pollution present in the operating
environment. Pollution degree 2 assumes that normally only nonconductive pollution, such as
dust, occurs with the exception of occasional conductivity caused by condensation.
Both of these affect the dimensioning of the electrical insulation within the instrument.
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8.3 In conformity with the requirements
The Multidrop 384 bears the following markings:
Type 832
100 ― 120 Vac, 200 ― 240 Vac 50/60 Hz, 50 VA
CE mark
CSA monogram
The Multidrop 384 conforms to the following requirements:
2006/95/EC (Low Voltage Directive)
2004/108/EC (Electromagnetic Compatibility Directive, EMC)
FCC Part 15, Subpart B/Class B
2002/96/EC (Waste of Electrical and Electronic Equipment)
Safety performance:
EN 61010-1:1993 + A2:1995/IEC 61010-1:1990 + A1:1992 + A2:1995
taking into account US and CA National differences
EN 61010-1:2001 (Ed. 2)
EMC performance:
EN 50081-1:1992 Generic emission standard. Residential, commercial and light industry.
EN 50082-1:1997 Generic immunity standard. Residential, commercial and light industry.
EN 61326-1:1997 + A1:1998 Product family standard.
+ A2:2001 + A3:2003
Test standards
and Performance limits
EN 55022:1998 Class B, 150 kHz ― 1 GHz
EN 61000-3-2:1995 + A1:1998 +
A2:1998 + A13:1997 + A14:2000
Class A
EN 61000-3-3:1995
ANSI C63.4:1992 Class B, 450 kHz – 1 GHz
EN 61000-4-2:1995 + A1:1998
4 kV CD, 8 kV AD, Criteria B
EN 61000-4-3:1996 + A1:1998 3 V/m, 80 MHz ― 2 GHz, Criteria A
EN 61000-4-4:1995 1 kV, Criteria B
EN 61000-4-5:1995 2 kV line to ground, 1 kV line to line, Criteria B
EN 61000-4-6:1996 3 V
rms
, 150 kHz ― 80 MHz, Criteria A
EN 61000-4-8:1994
EN 61000-4-11:1994
3 A/m, Criteria A
30%/10 ms, Criteria B
60%/100 ms, Criteria C
100%/20 ms, Criteria B
> 95%/5 s, Criteria C
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8.4 Remote control to Multidrop 384
The interface system consists of the serial RS-232C computer port which is used for computer control.
8.4.1 Hardware for the computer port
The character length is 10 bits with 1 start bit, 8 data bits, 1 stop bit and no parity. The format cannot
be changed.
The following signals are connected to the computer port DB-25 connector at the rear of the
instrument:
DB-25 CONNECTOR
Pin no. Signal name
2 TxD
3 RxD
4 RTS
7 GND
20 DTR
Signal description
2: TxD, transmit data (output)
3: RxD, receive data (input)
7: GND, signal ground
4/20: RTS/DTR (handshake lines)
The handshake lines
:
RTS/DTR are not in use in the standard Multidrop 384.
8.4.2 Multidrop 384 PC interface
Baudrate
9600
Parity
None
Data bits
8
Stop bits
1
Handshake
XON/XOFF
Commands
Commands to the Multidrop 384 have two to three fields as described in the following syntax. The first
letter identifies the command. Then a numeric argument field is optional for some and required for
other commands. The numeric field immediately follows the uppercase letter without any separating
characters. The last character of a command is a linefeed character <LF> or a carriage return
character <CR>. Commands may also be terminated to <CR><LF> or <LF><CR>, since the
instrument ignores empty commands.
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In the following list of commands, the command arguments in square brackets are optional.
D<LF> Dispense the volume set by the 'V' command to the entire plate.
Primes 10 µl into the priming vessel before dispensing.
E<LF> Empty the pump. A volume of 880 µl is pumped backwards.
G<LF> Dispense the plate using parameters from the plate switch and the volume/columns
thumbwheels. This command has the same effect as pressing the
start key. This
command has been added to version 1.7.
M[c]<LF> Dispense the given number of columns starting from the current column. If the column
count is not given, one column is dispensed. The dispensing volume is set by the 'V'
command. After the command is completed, the dispensing tips remain at the last
column dispensed. If this command is received when the plate is in the home position,
dispensing starts from column 1. ER3 is reported if more columns are requested than left
over from the current column to the last column of the plate.
N<LF> Report the internal software version of the instrument.
O<LF> Drive the plate out to the priming position.
P[v]<LF> Prime the given volume. For a 96-well plate the volume must be in the range of 5 to
1000 µl. For a 384-well plate the range is 5 to 100 µl. The volume must be dispensed in
5 µl increments. If the volume is not given, 200 µl is primed. If the plate is not in the
home position when the command is received, it is first driven into home position.
Q<LF> Reset the instrument. There is no response to this command.
S[c]<LF> Drive the requested column under the dispensing tips. Valid columns are 1 to 12 for a
96-well plate and 1 to 24 for a 384-well plate. If the column is not given, the steps are
one column forward or the plate returns to home position after the last column.
Tt<LF> Set the plate type assumed for all other commands except the ‘G’ command. At startup
the default type is read from the plate switch.
t = 0 96-well plate
t = 1 384-well plate
This command has been added to version 1.7.
V<LF>
VER<LF>
Report the internal software version of the instrument just like the ‘N’ command.
Vv<LF> Sets the volume for dispensing. For a 96-well plate the volume must be in the range of
5 to 1000 µl. For a 384-well plate the range is 5 to 140 µl. The volume must be
dispensed in 5 µl increments.
Zs<LF> Shake the plate the desired amount of time. The time is seconds and may range from 1
to 60.
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Responses and error codes
The instrument sends a response to each command after the command has been executed. The only
exception is the 'Q' command, for which there is no response.
The response to an 'N' command is as follows:
Mdrop384 Vr.l[-b]<CR><LF>
where:
r is the release number;
l is the level number, and
b is the optional branch number.
The response to other commands, if the command is executed successfully, is as follows:
OK<CR><LF>
If an error is detected, the response is one of the following:
ER3<CR><LF> Unrecognized command or invalid command argument.
ER4<CR><LF> The pump is not primed.
ER5<CR><LF> The priming vessel is not inserted into its slot.
ER6<CR><LF> Hardware error. The instrument stops and must be reset manually.
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9 TROUBLESHOOTING
9.1 Troubleshooting guide
The problems covered below are considered as faults that require repair or corrective work. If the installation
procedure is carefully followed, no faults should arise. However, if problems do occur or reoccur, contact
authorized technical service immediately.
Error Cause Action
No power to the
instrument
The instrument has not been
connected to the mains.
A fuse has blown.
Connect the instrument to the
mains.
Replace the blown fuse, refer to
section 7.4.
The keys do not
function
Check that the rotor cover is over
the rotor.
Check that the priming vessel is
properly inserted into its slot.
Drop on the end of the
tip exceptionally large
The tip is defective or clogged.
The tube is defective.
The dispensing cassette is not
properly installed.
Clear the tip with a needle or
replace the tip strip.
Replace the tubes.
Reinstall the cassette.
Large air bubbles in
the tubes
The instrument is not primed. Prime, refer to section 6.7.
Poor dispensing
accuracy and
precision
The dispensing cassette is not
properly installed.
The tubes are worn out.
There is friction on one of the rotor
needles. Bearing malfunction.
Reinstall the cassette.
Replace the tubes.
The rotor needles should spin
without any friction. If even a slight
friction is present, contact
authorized technical service.
Liquid being
dispensed does not
fall into the required
well of the microplate
The dispensing cassette is not
properly installed.
The tip is defective or clogged.
Reinstall the cassette.
Clear the tip with a needle or
replace the tip strip.
Plate carrier moves
incorrectly
There is foreign matter in the way of
the carrier.
The drive band of the drive wheel is
worn out or broken.
The drive wheel is loose.
The drive wheel is not properly
adjusted.
Remove the foreign matter.
Contact authorized technical
service.
Contact authorized technical
service.
Contact authorized technical
service.
Instrument does not
function
Contact authorized technical
service.
The error LED
operates when trying
to
prime
The priming vessel is not present.
There is dirt in the priming vessel
sensor.
Insert the priming vessel.
Clean the priming vessel sensor.
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9.2 FAQs about the Multidrop 384
Q1: Can the same dispensing cassette be used in all Multidrop models?
A1: No, use only dispensing cassettes designed for Multidrop 384. It is not possible to use small tube
dispensing cassettes.
Q2: How many times can the dispensing cassette be autoclaved?
A2: Fifty times.
Q3: What are the autoclaving conditions for the dispensing cassette?
A3: The autoclaving conditions are as follows: 1 bar pressure at 121°C for 20 min.
Q4: What is the dead volume of the standard 40 cm tubing set?
A4: The dead volume is about 5.6 ml. Note that you do not waste the reagent due to the special
backflush feature of the Multidrop 384, with which you can empty your reagents from the tubing back
into the original container.
Q5: What liquid is recommended to be used for washing the Multidrop 384 tubes?
A5: It is recommended you use deionized distilled water and/or detergent solutions, for example,
0.2 – 1% Tween followed by deionized distilled water.
Q6: What is the material of the Multidrop 384 priming vessel?
A6: The Multidrop 384 priming vessel is made of black polypropylene (PP).
Q7: What is the material of the dispensing cassette tip band?
A7: The tip band (Fig. 7.14) material is polypropylene (PP). The melting temperature of the material is
160 ― 165°C.
Q8: Can ultraviolet light be used for sterilization?
A8: Ultraviolet rays can be used to sterilize objects, for example, the surface of the Multidrop 384
instrument and other areas where destruction of bacteria is necessary.
Q9: When should I calibrate the dispensing cassette?
A9: The dispensing cassette needs to be calibrated when the silicone tubes have been changed. It is
also recommended you recalibrate the dispensing cassette after dispensing about 1000 96-well plates
or after every three months. The dispensing cassette must also be recalibrated if it has been forgotten
under tension for prolonged periods, for example, a weekend or longer.
Q10: What is the dead volume of the 2 m tubing set?
A10: The dead volume is about 24 ml.
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9.3 Hazards
This instrument is designed to provide full user protection. When correctly installed, operated and
maintained, the instrument will present no hazards to the user.
The following recommendations are given to ascertain added user safety.
9.3.1 Electrical
Ensure that the mains supply cable supplied with the unit is always used. If a correct type of mains
cable is not provided, use only cables certified by the local authorities.
The mains plug should only be inserted into a socket outlet provided with a protective ground contact.
The protective action must not be negated by use of an extension cable without a protective ground
wire.
When the instrument is connected to the mains supply, the opening of covers or removal of
components is likely to expose live parts. The instrument should be disconnected from all voltage
sources by disconnecting the mains supply cable, before it is opened for any adjustment,
replacement, maintenance or repair purposes.
Any adjustment, maintenance or repair of the opened instrument under voltage should be avoided, if
possible, but if unavoidable, should only be carried out by a skilled technician aware of the hazard.
Voltages dangerous for human beings are present in this instrument. Before removing any
covers, disconnect the instrument from the power supply.
The same precautions applicable when using any electrical equipment should certainly be observed
with this instrument. Do not touch switches or electrical outlets with wet hands. Switch the instrument
off before disconnecting it from the mains supply.
9.3.2 Mechanical
The dispensing cassette is a free-moving mechanical device controlled by the instrument and not
directly by the operator. It is designed for ‘hands-off’ operation and should be used as such. Never
reach into the work space, while the instrument is performing a dispensing cycle. If it is
necessary to stop the operation of the equipment, press the
stop key on the control panel.
9.3.3 Environmental
Infectious samples and corrosive fluids are commonly used with this equipment. The ‘hands-off’
nature of the system allows the user to dispense into the reaction wells without getting into direct
contact with these fluids. However, the wells that have been in contact with potentially hazardous
fluids must be handled before and after the dispensing process, and this should be done with utmost
care. Hand and eye protections should always be worn as well as corrosive resistant laboratory coats.
Observe normal laboratory procedures for handling potentially hazardous samples.
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9.3.4 Defects and abnormal stresses
Whenever it is likely that the protection against safety hazards has been impaired, the instrument
should be made inoperative and be secured against any unintended operation.
The protection is likely to be impaired if, for example, the instrument:
1. shows visible damage;
2. fails to perform the intended functions;
3. has been subjected to prolonged storage under unfavorable conditions, or
4. has been subjected to severe transport stresses.
9.4 Service request protocol
If the Multidrop 384 requires service, contact your local Thermo Fisher Scientific representative or the
Thermo Fisher Scientific technical service department. Do not under any circumstances send the
instrument for service without any prior contact. It is imperative to indicate the fault and nature of the
required service. This will ensure a faster return of the instrument to the customer.
The Thermo Fisher Scientific representative or distributor will take care of sending a complaint form
(that is, the Warranty Claim Technical Sheet) to the Thermo Fisher Scientific technical service
department. The Warranty Claim Technical Sheet contains a more detailed description of the fault,
symptom or condition. Give all the necessary information to the distributor, who will fill in and forward
the Warranty Claim Technical Sheet to the Thermo Fisher Scientific technical service department.
Check section 7.9 How to pack for service. You will find instructions on how to proceed before
shipping the instrument for service.
Check that any necessary decontamination procedure has been carried out before packing. See
sections 9.4.1 and 9.4.1.1 on decontamination. Refer also to section 7.8 Decontamination procedure.
Ensure that the Certificate of Decontamination as well as the return authorization number (RGA) are
sent with the instrument.
The Thermo Fisher Scientific technical service department will keep you up to date with the progress
of service and provide you with any further details you might need, for example, on maintenance,
serviceability, troubleshooting and replacement.
9.4.1 Decontamination
Decontamination should be performed in accordance with normal laboratory procedures. Any
decontamination instructions provided with the reagents used should be followed.
A decontamination procedure is only recommendable when infectious substances have been in direct
contact with any part(s) of the instrument.
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9.4.1.1 Certificate of Decontamination
The decontamination procedure is required prior to shipping the instrument to Thermo Fisher Scientific
Oy, for example, for repair. If, for any reason, the instrument is shipped back to Thermo Fisher
Scientific Oy, it must be accompanied by a dated and signed Certificate of Decontamination, which
must be attached to the outside of the package containing the instrument. See section 7.8
Decontamination procedure.
Failure to confirm decontamination will incur additional labor charges or at worst the items will be
returned for proper cleaning.
Before returning any instrument(s) or item(s), ensure that they are fully decontaminated. Confirm A or
B status:
Name:
Address:
Tel./Fax:
Name: Serial no.:
A)
I confirm that the returned items have not been contaminated by body fluids, toxic, carcinogenic or radioactive
materials or any other hazardous materials.
B)
I confirm that the returned items have been decontaminated and can be handled without exposing the personnel
to health hazards.
Materials used in the unit: Chemicals + Biological • Radioactive *)
Specific information
about contaminants:
Decontamination
procedure
1
:
Date and place
:
Signature
:
Name (block capitals)
:
*) The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive
materials.
This unit is certified by the undersigned to be free of radioactive contamination.
Date and place
:
Signature
:
Name (block capitals)
:
1
Please include decontaminating solution used.
PHOTOCOPIABLE
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10 WARRANTY CERTIFICATE
10.1 Warranty Certificate
Thermo Fisher Scientific Microplate Instrumentation Business products are fully guaranteed against
defective parts and materials, including defects caused by poor workmanship, for a period of one year
from the date of delivery.
Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty
at no extra charge for materials and labor provided that the products were used and maintained in
accordance with Thermo Fisher Scientific’s instructions. The warranty is invalid if products have been
misused or abused.
For the warranty to be effective, the product must have been purchased either directly from Thermo
Fisher Scientific or from an authorized Thermo Fisher Scientific distributor. The guarantee is not
transferable to a third party without prior written approval from Thermo Fisher Scientific.
This guarantee is subject to the following exclusions:
• Any defects caused by normal wear and tear.
• Defects caused by fire, lightning, flood, earthquake, explosion, sabotage, war, riot, or any
other occurrence of the type listed above.
• Refurbished products that are subject to different warranty conditions.
THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES,
INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR
FITNESS FOR A PARTICULAR PURPOSE. The seller is not liable for any loss or damage arising out
of or in connection with the use of the product or other indirect damages.
These warranty terms and conditions can be obtained from your local Thermo Fisher Scientific dealer.
Note: Consumables are not included in the warranty.
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11 ORDERING INFORMATION
11.1 Instruments
Code Instrument
5840150 Multidrop 384, 220 – 240 V 50/60 Hz
5840157 Multidrop 384, 110 – 120 V 50/60 Hz
11.2 Dispensing cassettes
Code Dispensing cassette
24072670 Standard tube dispensing cassette (with 40 cm tubing set)
24072671 Standard tube dispensing cassette 5-pack
24072672 Standard tube dispensing cassette 10-pack
24072677 Long standard tube dispensing cassette (length of tubing 50 – 200 cm in 10 cm
increments, please specify the length when ordering)
24070300 Dispensing cassette Machined covers (with 40 cm tubing set) (Fig. 11.1)
24070307 Special dispensing cassette Multidrop, Machined covers (length of tubing 50 – 200 cm in
10 cm increments, please specify the length when ordering)
Fig. 11.1 Dispensing cassette Machined covers
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11.3 Spare parts for dispensing cassettes
Code Item
24070290 Tubing set for dispensing cassette (with 40 cm tubing set)
24070297 Special tubing set for dispensing cassette, special tubing set Multidrop (length of tubing
50 – 200 cm in 10 cm increments, please specify the length when ordering)
1046200 Tube tip manifold
1190780 Tubing weight, eight tubes
1002430 Tubing weight, one tube
2805980 Tube holder assembly
11.4 Priming vessels (consumables)
Code Priming vessel
1046240 Priming vessel, one cavity
1046241 Priming vessel, eight cavities
10260315 Self-draining priming vessel
11.5 List of accessories
Code Item
1507010 Multidrop 384 User Manual
2406910 Volumetric calibration device
1610641 Dust cover
24071790 Plate adapter (max. 15 mm height)
24071800 Plate adapter (max. 11 mm height)
2305290 Serial cable F9/F25
11.6 List of plates (consumables)
Code Item Qty
3355
96-well microplates
Immulon 1B Plate, Flat bottom
50 pcs/box
9502227 96 Well Plate UB, Flat bottom 50 pcs/box
7571 Microlite 1 + Plate, Flat bottom 50 pcs/box
9502887 White 96 Well Plate UB, Flat bottom 50 pcs/box
7605 Microfluor 1 Black Plate, Flat bottom 50 pcs/box
9502867 Black 96 Well Plate UB, Flat bottom 50 pcs/box
6310 Immulon 1 B 1x12 Strip Plate, Flat bottom 100 pcs/box
384-well microplates
95040000 384 Round Well Plate 50 pcs/box
95040020 White 384 Round Well Plate 50 pcs/box
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11.7 List of spare parts
Code Item Qty
1210810 Fuse 300 mA 10 pcs/box
1210850 Fuse 600 mA 10 pcs/box
11.8 List of recommended spare parts
Cat. no. Item 1 ― 2 unit(s) / year 10 units / year
10478101 Carriage 1
1093270 Tooth belt 1 4
1100420 Transfer rail 2
1190760 Tension limiting wire 4 6
1210810 Fuse 0.6 A slow 5 x 20 (115 V) 1 1
1210850 Fuse 0.3 A slow 5 x 20 (230 V) 1 1
2003350 MUKEY PCB 1
2004330 MUDROP-03 PCB 1
2004340 MUBCD-02 PCB (384) 1 3
2304720 Microswitch assembly 1
2304860 Flat cable 1
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12 GLOSSARY AND ABBREVIATIONS
dispense
To distribute (pipette) liquid into the wells of the preselected strips or microplate.
dispensing
cassette
The dispensing cassette contains eight individual tubes. The dispensing cassette is
detachable. Each reagent can have a separate dispensing cassette. Alternatively,
the same dispensing cassette can be used with several reagents using the
backflush feature. Refer to section 11.2 Dispensing cassettes.
The dispensing cassettes contain either Machined or Extruded covers. The tubing
length also varies from 40 cm to a selectable length of 50 to 2000 cm.
All dispensing cassettes can be autoclaved up to 50 times.
To avoid damaging the tubes, follow the dispensing cassette maintenance,
washing, autoclaving and storage instructions carefully. Refer to sections 6.8.4
After use, 7.2 How to wash the dispensing cassette and 7.3 How to autoclave the
dispensing cassette.
EIA
enzyme immunoassay
empty
The operation of discharging the contents of the tubing of fluid.
EN
European Norm
EU
European Union
FAQ
frequently asked questions
home position
The plate carrier is positioned to the furthest right of the transfer rails (Fig. 4.1).
IEC
International Electrotechnical Commission
plate adapter
The Multidrop 384 requires a plate adapter for the plate carrier to function.
Two different plate adapters are included:
the PLATE HEIGHT MAX. 15 MM plate adapter is suitable for plates approximately
15 mm high and the PLATE HEIGHT MAX. 11 MM plate adapter is suitable for
lower plates.
Insert the plate adapter correctly. Refer to section 6.4 How to insert and adjust the
plate adapter.
PP
polypropylene
prime
The operation of filling a pump intake with fluid to expel the air.
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12.1 Keywords for web pages
automated dispensers microwell plate
automated fluid dispensing multidispense
automated liquid handling multidrop
dispensing Multidrop
drug discovery Multidrop 384
EIA multidrops
ELISA plate
enzyme immunoassay robotic platform
immunoassay sandwich assay
liquid handling separation technique(s)
microplate solid phase assay(s)
microplate dispenser Thermo Fisher Scientific
Microtiter plate Thermo Scientific
12.2 Literature
Editorial (2004): Multidrop bulk reagent dispenser family from Thermo Electron; precise dispensing in
the microliter area allowing assay miniaturization. Innovations in Pharmaceutical Technology 4 (14),
36.
Ennis, C. and Tanner, A. (Fall 1999): Analysis of CHO cell requirements for assay miniaturization in
high-throughput screening. The HTS Forum 8, 5 pages.
Falconer M. et al. (2002): High-Throughput Screening for Ion Channel Modulators. Journal of
Biomolecular Screening 7 (5), 460 – 465.
Hudson Control Group (Jan 2001): Integrating the Thermo Labsystems Multidrop with the
PlateCrane™. IB104A, 3 pages.
Kiger A. A. et al. (2003): A functional genomic analysis of cell morphology using RNA interference.
Journal of Biology 2, 27.
Tanaka M. et al. (2005): An unbiased cell morphology-based screen for new, biologically active small
molecules. PLoS Biology 3 (5), e128.
Yarrow J. C. et al. (2004): A high-throughput cell migration assay using scratch wound healing, a
comparison of image-based readout methods. BMC Biotechnology 4, 21.
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13 INDEX
A
Abbreviations......................................................................................................................................... 75
Accessories ............................................................................................................................... 15, 16, 73
Addresses ............................................................................................................................................. 85
Advantages of using the Multidrop 384................................................................................................... 9
Application ........................................................................................................................... 5, 6, 9, 76, 84
B
Bottle ....................................................................................................................................................... 9
C
Cable ............................................................................................... 15, 17, 18, 19, 57, 61, 68, 73, 74, 81
Calibration .................................12, 24, 32, 33, 40, 41, 42, 43, 44, 46, 47, 51, 52, 53, 55, 60, 73, 81, 82
screw cover.................................................................................................... 12, 41, 42, 43, 44, 53, 55
Channels ....................................................................................................................... 40, 42, 43, 44, 81
Check ......................................................................15, 16, 17, 18, 19, 21, 23, 24, 32, 33, 39, 66, 69, 83
Clean ..................................................................................................... 16, 34, 35, 36, 37, 46, 58, 66, 70
Col ............................................................................................................... 14, 19, 30, 31, 33, 60, 64, 83
Columns ................................................................................................10, 14, 19, 30, 31, 33, 60, 64, 83
Consumables ............................................................................................................................ 32, 71, 73
Control panel ............................................................................. 10, 19, 21, 30, 31, 36, 60, 68, 81, 82, 83
indicators ........................................................................................................................................... 30
keys.................................................................................................................................................... 31
Cover retaining screws.................................................................... 11, 17, 18, 45, 47, 49, 53, 54, 81, 82
D
Dead volume ......................................................................................................................................... 67
Decontamination ....................................................................................................................... 58, 69, 70
Certificate of........................................................................................................................... 58, 69, 70
Decontamination procedure .......................................................................................... 35, 57, 58, 69, 70
Dimensions............................................................................................................................................ 60
Directives............................................................................................................................................... 62
Disclaimer................................................................................................................................................ 5
Dispenser .................................................................................................... 7, 8, 9, 14, 15, 19, 30, 35, 76
Dispensing. 7, 8, 9, 11, 12, 14, 16, 19, 21, 22, 23, 24, 25, 29, 30, 31, 32, 33, 34, 35, 36, 37, 40, 42, 43,
45, 47, 48, 49, 50, 52, 53, 54, 55, 56, 57, 58, 60, 64, 66, 67, 68, 72, 73, 75, 76, 81, 82, 83
accuracy ............................................................................................................................................ 60
cassette.. 7, 8, 9, 11, 12, 14, 16, 19, 21, 22, 23, 24, 25, 29, 34, 35, 36, 37, 40, 42, 43, 45, 47, 48, 49,
50, 52, 53, 54, 55, 56, 57, 58, 60, 66, 67, 68, 72, 73, 75, 81, 82, 83
precision ............................................................................................................................................ 60
volume ......................................................................................................................................... 60, 64
Disposal of instrument........................................................................................................................... 58
Disposal of materials ............................................................................................................................. 57
E
Empty ..........................................................19, 21, 31, 32, 33, 34, 35, 42, 43, 57, 60, 63, 64, 67, 75, 83
Environmental requirements ................................................................................................................. 16
Error................................................................................................................... 19, 30, 31, 32, 35, 65, 66
code ................................................................................................................................................... 65
Extruded covers .............................................................................................................. 8, 12, 72, 75, 81
F
FAQ ................................................................................................................................................. 67, 75
Frequently asked questions .................................................................................................................. 67
Fuse holder cap................................................................................................................... 13, 38, 39, 81
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Fuses....................................................................................................................... 13, 38, 39, 66, 74, 81
G
Gravimetric calibration..................................................................................................................... 40, 42
Guidelines for use ................................................................................................................................. 35
H
Hazard ............................................................................................................................................... 6, 68
Home position ............................................................................................. 14, 19, 24, 31, 33, 35, 64, 75
How to autoclave the dispensing cassette................................................................................ 14, 37, 75
How to change the tip band .................................................................................................................. 45
How to disinfect the instrument ........................................................................................... 35, 57, 69, 70
How to dispense automatically.............................................................................................................. 32
How to dispense manually into the specified column(s) ....................................................................... 33
How to dispense multiple volumes........................................................................................................ 33
How to fit the new tubing set ................................................................................................................. 46
How to install the Multidrop 384 .............................................................................. 15, 17, 19, 20, 21, 61
How to pack for service ............................................................................................................. 57, 58, 69
How to prime ................................................................................................................................... 32, 43
How to recalibrate the dispensing cassette .................................................................................... 40, 56
How to replace the fuses....................................................................................................................... 38
How to select the 96/384 well plate....................................................................................................... 31
How to shutdown........................................................................................................... 14, 34, 35, 37, 75
How to switch on ................................................................................................................................... 21
How to troubleshoot the instrument ...................................................................................................... 66
How to use the control panel........................................................................................................... 19, 30
How to wash the dispensing cassette....................................................................................... 14, 37, 75
I
Indicators ............................................................................................................................................... 30
Installation ............................................................................................... 7, 15, 16, 17, 40, 42, 47, 61, 66
check list .....................................................................................................................
....................... 15
Instrument layout................................................................................................................................... 10
Intended use............................................................................................................................................ 8
K
Keys............................................................................................................. 14, 30, 31, 33, 35, 41, 60, 66
Keywords for web pages ....................................................................................................................... 76
L
LED................................................................................................................................ 19, 21, 30, 35, 66
Literature ............................................................................................................................................... 76
Live parts ............................................................................................................................................... 61
Lower part........................................................................................ 10, 12, 22, 23, 24, 45, 47, 48, 49, 81
M
Machined covers ............................................................................................................................. 72, 82
Mains ........................................................... 11, 13, 15, 17, 18, 19, 21, 35, 38, 39, 57, 60, 61, 66, 68, 81
switch ................................................................................................................... 11, 17, 18, 19, 21, 35
Maintain ............................................................................................................................... 21, 36, 58, 83
Maintenance................................................................................ 7, 14, 21, 35, 36, 58, 61, 68, 69, 75, 83
Microplate dispenser ............................................................................................... 7, 8, 9, 14, 35, 76, 81
Microstrip ......................................................................................................................... 5, 43, 44, 57, 81
Microswitch...................................................................................................................................... 24, 74
Mode.............................................................................................................................. 14, 31, 32, 33, 41
Multidrop 384....7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 24, 25, 30, 31, 32, 33, 35, 36, 40, 41, 42, 43, 61, 62,
63, 67, 69, 72, 73, 74, 75, 76, 81, 82, 83
O
Operating precautions and limitations................................................................................................... 20
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Operational check ..................................................................................................................... 15, 19, 21
list....................................................................................................................................................... 21
Ordering information................................................................................................................................ 7
P
Packing...................................................................................................................................... 15, 58, 69
instructions................................................................................................................................... 15, 58
list....................................................................................................................................................... 15
materials ............................................................................................................................................ 58
Plate .... 7, 8, 9, 10, 11, 14, 19, 21, 22, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 40, 41, 42, 43,
44, 45, 50, 53, 55, 57, 60, 64, 66, 67, 73, 74, 75, 76, 81, 82, 83
adapter............................................... 10, 11, 21, 25, 26, 27, 28, 29, 32, 33, 42, 43, 57, 73, 75, 81, 83
adapter lock ..................................................................................................................... 10, 26, 27, 81
carrier...............................10, 11, 14, 19, 21, 22, 24, 25, 26, 27, 28, 29, 30, 32, 33, 35, 36, 66, 75, 81
switch ................................................................................................................... 10, 19, 21, 31, 43, 64
type .................................................................................................................................. 21, 31, 64, 83
Polypropylene tubing....................................................................................................................... 67, 75
Precalibration ............................................................................................................................ 55, 56, 82
Prime .... 10, 19, 21, 22, 24, 31, 32, 33, 34, 35, 37, 40, 41, 42, 43, 58, 60, 64, 65, 66, 67, 73, 75, 81, 83
Prime/drop ......................................................................................................... 19, 32, 33, 41, 43, 60, 83
Priming vessel ...................................................10, 19, 21, 22, 31, 32, 33, 40, 42, 65, 66, 67, 73, 81, 83
Protocol ................................................................................................................................................. 69
Pump body ...................................................................................................................................... 10, 23
Purge ..................................................................................................................................................... 75
R
Remote control .................................................................................................................................. 9, 63
Rest position.................................................................................... 10, 21, 25, 34, 35, 37, 40, 43, 81, 83
Robotic ........................................................................................................................................ 9, 29, 76
Robotics........................................................................................................................................... 29, 76
Rocker switch ...................................................................................................................... 10, 21, 31, 43
Rotor............................................................10, 11, 19, 21, 23, 24, 25, 29, 32, 34, 35, 40, 42, 66, 81, 83
cover .......................................................................... 10, 11, 19, 21, 24, 25, 29, 32, 40, 42, 66, 81, 83
needles .................................................................................................................................. 10, 24, 66
shaft ............................................................................................................................................. 23, 24
Routine ............................................................................................................................................ 21, 36
Run ........................................................................................................................................................ 83
S
Samples..................................................................................................................................... 16, 57, 68
Service..................................................................................................................... 36,
37, 57, 58, 66, 69
contracts ............................................................................................................................................ 58
pack for .................................................................................................................................. 57, 58, 69
request protocol ................................................................................................................................. 69
Shutdown............................................................................................................................................... 35
Sight strips................................................................................................................................. 12, 55, 56
Sight window ............................................................................................................................. 50, 51, 81
Silicone tubing ........................................................................................................................... 32, 47, 67
Slots..................................................................................................................................... 22, 32, 65, 66
Software ............................................................................................................................................ 7, 64
Spare parts...................................................................................................................................... 73, 74
recommended.................................................................................................................................... 74
Specifications ........................................................................................................................ 7, 29, 60, 61
general............................................................................................................................................... 60
safety ................................................................................................................................................. 61
Startup ....................................................................................................................................... 18, 21, 64
Step ............................................................................................................. 14, 19, 31, 33, 35, 41, 46, 60
Switching on .......................................................................................................................................... 18
Symbols................................................................................................................................................... 6
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T
Table of Figures .................................................................................................................................... 81
Tension limiting wires .................................................................................. 12, 23, 24, 47, 48, 52, 74, 81
Thumbwheels ........................................................................................................................................ 33
Tip................................................................................................................ 40, 45, 46, 47, 66, 67, 73, 81
band ........................................................................................................................... 45, 46, 47, 67, 81
manifold ........................................................................................................................... 45, 47, 73, 81
Transfer rails ............................................................................................................................. 10, 74, 75
Transport ....................................................................................................................... 15, 16, 58, 60, 69
damage........................................................................................................................................ 15, 16
package ................................................................................................................................. 15, 16, 58
Troubleshooting............................................................................................................................... 66, 69
guide .................................................................................................................................................. 66
Tubes.......................................................................32, 33, 40, 42, 43, 45, 47, 48, 51, 55, 56, 66, 73, 81
Tubing........................8, 12, 19, 25, 32, 33, 34, 35, 45, 46, 47, 48, 50, 51, 52, 56, 67, 72, 73, 75, 81, 82
identification plate ........................................................................................................................ 50, 81
set .................................................................................................. 8, 25, 45, 46, 47, 56, 67, 72, 73, 81
weight....................................................................................................... 12, 32, 33, 46, 47, 56, 73, 82
U
Unpack .................................................................................................................................................. 15
Upper part............................10, 12, 22, 23, 24, 34, 35, 40, 41, 42, 43, 47, 49, 50, 52, 53, 54, 55, 81, 82
User manual ................................................................................................................................ 7, 15, 73
V
View..................................................................................................................................... 10, 11, 13, 14
front........................................................................................................................................ 10, 11, 81
internal ............................................................................................................................................... 14
rear............................................................................................................................................... 13, 81
Voltage .................................................................................................................... 16, 17, 20, 62, 68, 81
selector ........................................................................................................................................ 17, 81
Volumetric calibration .............................................................................................. 32, 33, 40, 41, 60, 73
W
Warnings ................................................................................................................................... 16, 17, 18
Warranty...................................................................................................................................... 7, 15, 71
Weight ...................................................................................................12, 16, 32, 33, 46, 47, 56, 60, 73
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14 TABLE OF FIGURES
FIG. 3.1 MULTIDROP 384 MICROPLATE DISPENSER ...................................................................................... 8
FIG. 4.1 MULTIDROP 384 FRONT VIEW (A)................................................................................................. 10
FIG. 4.2 MULTIDROP 384 FRONT VIEW (B)................................................................................................. 11
FIG. 4.3 MULTIDROP 384 FRONT VIEW (C) ................................................................................................ 11
FIG. 4.4 CLOSE-UP OF THE MULTIDROP 384 STANDARD TUBE DISPENSING CASSETTE.................................. 12
FIG. 4.5 MULTIDROP 384 REAR VIEW ........................................................................................................ 13
FIG. 4.6 MULTIDROP 384 MICROPLATE DISPENSER .................................................................................... 14
FIG. 5.1 LOCATION OF THE COVER RETAINING SCREWS .............................................................................. 17
FIG. 5.2 LOCATION OF THE VOLTAGE SELECTOR ........................................................................................ 17
FIG. 5.3 CONNECTING THE MAINS SUPPLY CABLE....................................................................................... 18
FIG. 6.1 INSERTING THE PRIMING VESSEL INTO PLACE ................................................................................ 22
FIG. 6.2 INSERTING THE STANDARD TUBE DISPENSING CASSETTE ............................................................... 22
FIG. 6.3 INSERTING THE LOWER PART OF THE DISPENSING CASSETTE INTO ITS SLOTS.................................. 23
FIG. 6.4 INSERTING THE UPPER PART OF THE DISPENSING CASSETTE.......................................................... 23
FIG. 6.5 LOWER AND UPPER PARTS OF THE DISPENSING CASSETTE INSERTED EVENLY INTO THEIR SLOTS ..... 24
FIG. 6.6 PULLING THE ROTOR COVER OVER THE ROTOR ............................................................................. 24
FIG. 6.7 TWO DIFFERENT PLATE ADAPTERS ............................................................................................... 25
FIG. 6.8 OPENING THE PLATE ADAPTER LOCK ............................................................................................ 26
FIG. 6.9 PLACING THE RIGHT END OF THE PLATE ADAPTER ......................................................................... 26
FIG. 6.10 PRESSING THE LEFT END OF THE PLATE ADAPTER ....................................................................... 27
FIG. 6.11 CLOSING THE PLATE ADAPTER LOCK .......................................................................................... 27
FIG. 6.12 LOOSENING THE SCREWS ON THE RIGHT OF THE PLATE CARRIER ................................................. 28
FIG. 6.13 LOOSENING THE SCREWS AT THE FRONT OF THE PLATE ADAPTER ................................................ 28
FIG. 6.14 FASTENING THE FRONT SCREWS OF THE PLATE ADAPTER ............................................................ 29
FIG. 6.15 FASTENING THE SCREWS TO THE RIGHT OF THE PLATE CARRIER .................................................. 29
FIG. 6.16 MULTIDROP 384 CONTROL PANEL .............................................................................................. 30
FIG. 6.17 MULTIDROP 384 CONTROL PANEL .............................................................................................. 31
FIG. 6.18 REST POSITION OF THE DISPENSING CASSETTE........................................................................... 34
FIG. 7.1 REMOVING THE FUSE HOLDER CAP (A) ......................................................................................... 38
FIG. 7.2 REMOVING THE FUSE HOLDER CAP (B) ......................................................................................... 38
FIG. 7.3 FUSE HOLDER CAP WITH FUSES ................................................................................................... 39
FIG. 7.4 INSERTING THE FUSE HOLDER CAP ............................................................................................... 39
FIG. 7.5 PLACING THE TUBE ONTO THE TIP OF CHANNEL A.......................................................................... 40
FIG. 7.6 ADJUSTING THE LIQUID LEVEL FIRST TO THE LOWER MARK AND THEN TO THE UPPER MARK .............. 41
FIG. 7.7 SCREWING THE CALIBRATION SCREWS ACCORDING TO THE CALIBRATION RESULTS ......................... 42
FIG. 7.8 8 X 12 WELL MICROSTRIPS ADJUSTED ONTO THE PLATE ADAPTER .................................................. 43
FIG. 7.9 REMOVING THE FILLED MICROSTRIP TO BE WEIGHED ..................................................................... 44
FIG. 7.10 SCREWING THE CALIBRATION SCREW ACCORDING TO THE CALIBRATION RESULTS ......................... 44
FIG. 7.11 REMOVING THE COVER PLATE OF THE LOWER PART OF THE DISPENSING CASSETTE ...................... 45
FIG. 7.12 REMOVING THE OLD TIP BAND .................................................................................................... 45
FIG. 7.13 TIP BAND REMOVED................................................................................................................... 46
FIG. 7.14 NEW TIP BAND .......................................................................................................................... 46
FIG. 7.15 NEW TUBING SET WITH THE TIP BAND ATTACHED ......................................................................... 47
FIG. 7.16 INSERTING THE TIP MANIFOLD INTO THE LOWER PART OF THE DISPENSING CASSETTE.................... 47
FIG. 7.17 PRESSING THE TUBE FASTENERS INTO PLACE ............................................................................. 48
FIG. 7.18 NEW TUBING INSERTED INTO THE LOWER PART OF THE DISPENSING CASSETTE ............................. 48
FIG. 7.19 TENSION LIMITING WIRES ATTACHED .......................................................................................... 48
FIG. 7.20 REPLACING THE LOWER PART COVER OF THE DISPENSING CASSETTE........................................... 49
FIG. 7.21 PARTS BELONGING TO THE UPPER PART OF THE DISPENSING CASSETTE....................................... 49
FIG. 7.22 PLACING THE SIGHT WINDOW..................................................................................................... 50
FIG. 7.23 PRESSING THE TUBING IDENTIFICATION PLATE INTO PLACE .......................................................... 50
FIG. 7.24 PRESSING THE TUBE FASTENERS INTO PLACE ............................................................................. 51
FIG. 7.25 FASTENING THE CALIBRATION SCREWS....................................................................................... 51
FIG. 7.26 CALIBRATION SCREWS FASTENED .............................................................................................. 52
FIG. 7.27 REPLACING THE COVER OF THE UPPER PART OF THE DISPENSING CASSETTE ................................ 52
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F
IG. 7.28 ENSURING THE TUBING DOES NOT GET TRAPPED......................................................................... 52
FIG. 7.29 FASTENING THE COVER RETAINING SCREWS ............................................................................... 53
FIG. 7.30 INSERTING THE PLATE ON THE CALIBRATION SCREWS .................................................................. 53
FIG. 7.31 LOWER AND UPPER PART OF THE DISPENSING CASSETTE FITTED ................................................. 54
FIG. 7.32 DISPENSING CASSETTE BEFORE PRECALIBRATION....................................................................... 55
FIG. 7.33 PRECALIBRATING THE DISPENSING CASSETTE............................................................................. 55
FIG. 7.34 DISPENSING CASSETTE PRECALIBRATED .................................................................................... 56
FIG. 7.35 INSERTING THE TUBES INTO THE TUBING WEIGHT ........................................................................ 56
FIG. 11.1 DISPENSING CASSETTE MACHINED COVERS ............................................................................... 72
FIG. APPENDIX 1. 1 MULTIDROP 384 CONTROL PANEL ............................................................................... 83
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APPENDIX 1. THERMO SCIENTIFIC MULTIDROP 384 BRIEF USER’S
GUIDE
• Insert the dispensing cassette to be used (p. 22).
• Check that the rotor cover is pulled over the rotor (p. 21).
• Check that the priming vessel is installed (p. 22).
• Use the correct plate adapter (p. 25).
• Switch the Multidrop 384 on (p. 21).
• Load the microplate to be dispensed onto the instrument (p. 25).
• Select the desired plate type, volume and column(s) from the control panel
(p. 30).
• To prime the tubes, press the prime/drop key until the reagent flows from the
tips into the priming vessel (p. 32).
• Press the start key to start dispensing (p. 31).
• After the end of the run, remove the microplate (p. 34).
• Press the empty key to remove the reagent from the dispensing cassette
(p. 34).
• Put the dispensing cassette into the rest position (p. 34).
• Maintain your Multidrop 384 instrument and dispensing cassette (p. 36).
• Switch off the instrument (p. 35).
Fig. Appendix 1. 1 Multidrop 384 control panel
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APPENDIX 2. THERMO SCIENTIFIC MULTIDROP 384 FEEDBACK
FORM
Cat. no. Serial no.
PURCHASED BY PURCHASED FROM
Company/Institute
Distributor
Department
Address
Address
Tel.
Tel.
Fax
Date of delivery
Internet home page
Date of purchase
Your research area
Dr. Mr. Mrs. Ms. Job title/Position
Surname (block capitals)
First name (block capitals)
Internet e-mail address
Excellent Above As Below Comments
expectations expected expectations
Reagent kit/Instructions
Instrument/User manual
Operational reliability
Design
Ease of use
Operational costs
Customer support
Further instrument/system developments desired:
Further applications desired:
Where did you first learn about the product?
Would you like to receive information about other Thermo Scientific products?
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APPENDIX 3. ADDRESSES
For the latest information on products and services, visit our websites at:
http://www.thermo.com
http://www.thermo.com/multidrop
Manufactured by: Distributed by:
Thermo Fisher Scientific Oy
P.O. Box 100, FI-01621 Vantaa, Finland
Tel. +358-9-329 100, Fax +358-9-3291 0415
www.thermo.com
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Thermo Fisher Scientific Oy
Microplate Instrumentation
Ratastie 2, P.O. Box 100
FI-01621 Vantaa
Finland
www.thermo.com
15017010
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