Thermo Scientific Locator Jr, Locator 4, Locator 6, Locator Jr Plus, Locator 4 Plus User Manual

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User Manual

Locator and Locator Plus

Cryogenic Storage Vessel with or without
Ultrasonic Level Monitor

Operation Manual and Parts List LT509X9 (7018141) Rev. 4

Thermo Scientific

MANUAL NUMBER LT509X9 (7018141)

4 40578 2/23/16 Clarified Do Not Roll warning ccs

3 -- 10/27/15 Updated symbols and labels, added cautions ccs

2 31623 5/15/15 Added caution for moving vessel in Section 3 ccs

1 31528 9/15/14 Updated conformity specs and declaration ccs

0 27564 1/31/14 Original - new PCBA design ccs

REV ECR/ECN DATE DESCRIPTION By

Preface

Cryogenic Storage Vessel i

Locator Catalog Number

Locator

Model

Name

CY50925 w/o Monitor 8141 Locator Jr

CY50935 w/o Monitor 8143 Locator 4

CY50985 w/o Monitor 8197 Locator 6

CY50945 w/o Monitor 8145 Locator 8

CY509105 w/ Monitor 8201 Locator Jr.

CY509105CN w/ Monitor 8202 Locator Jr. China

CY509107 w/ Monitor 8246 Locator 4

CY509107CN w/ Monitor 8247 Locator 4 China

CY509113 w/ Monitor 8198 Locator 6

CY509110 w/ Monitor 8211 Locator 8

CY509110CN w/ Monitor 8212 Locator 8 China

Locator Plus Catalog Number

Locator Plus

Model

Name

CY50925-70 w/o Monitor 8142 Locator Jr Plus

CY50935-70 w/o Monitor 8144 Locator 4 Plus

CY50985-70 w/o Monitor 8147 Locator 6 Plus

CY50945-70 w/o Monitor 8146 Locator 8 Plus

CY509106 w/ Monitor 8237 Locator Jr Plus

CY509106CN w/ Monitor 8238 Locator Jr Plus China

CY509108 w/ Monitor 8207 Locator 4 Plus

CY509108CN w/ Monitor 8208 Locator 4 Plus China

CY509109 w/ Monitor 8209 Locator 6 Plus

CY509109CN w/ Monitor 8210 Locator 6 Plus China

CY509111 w/ Monitor 8213 Locator 8 Plus

CY509111CN w/ Monitor 8214 Locator 8 Plus China

Thermo Scientificii Cryogenic Storage Vessel

Preface

Important Read this instruction manual. Failure to read, understand and follow instructions in this manual may
result in damage to the unit, injury to operating personnel, and poor equipment performance.

s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Caution It is recommended to have this vessel tested by the manufacturer or qualified cryovessel service

technician every 7-10 years, regardless of any problems (or lack thereof) you may have had in the past.
This will help insure your samples against sudden loss of liquid nitrogen due to vacuum failure.

s

Warning Nitrogen gas can cause suffocation without warning. Store and use liquid nitrogen only in a
well-ventilated place. As the liquid evaporates, the resulting gas displaces the normal air in the area.
(The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture, not
the gas itself. The issuing gas is invisible.) In closed areas, excessive amounts of nitrogen gas reduces the
concentration of oxygen and can result in asphyxiation. Because nitrogen gas is colorless, odorless, and
tasteless, it cannot be detected by the human senses. Breathing an atmosphere that contains less than

19.5% oxygen can cause dizziness and quickly result in unconsciousness and death. Therefore, the use
of oxygen monitoring equipment is strongly recommended.

s

Warning Contact of liquid nitrogen with the skin or eyes may cause serious (freezing) injury. s

Warning Do not roll. Always keep liquid nitrogen vessel in an upright position. Exercise caution to

prevent spilling and splashing.

s

Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.

Intended Use:

The Thermo Fisher Scientific cryogenic storage vessels are intended to store biological samples at cryogenic
temperatures. These products are comprised of a vessel and a lid, with or without a level monitor.

Unintended Use:

These products are not intended for use within the patient environment.

When this manual is translated into other languages, the US English version is binding.

© 2010 Thermo Fisher Scientific. All rights reserved. Printed in U.S.A.

CAUTION

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

Thermo Scientific Cryogenic Storage Vessel iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.

Extreme temperature hazards. Only qualified persons should perform procedures associated with this
symbol.

Potential biological hazards. Proper protective equipment and procedures must be used.

Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.

This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo Scientific products will be available at www.thermoscientific.com.

4 Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4 Always dissipate extreme cold or heat and wear protective clothing.

4 Always follow good hygiene practices.

4 Each individual is responsible for his or her own safety.

Thermo Scientificiv Cryogenic Storage Vessel

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermoscientific.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address

Certified Service Web Pagewww.unitylabservices.com

Our staff can provide information on pricing and give you quotations. We canSales Support

take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.

When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:

Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road, Box 649
Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

Cryogenic Storage Vessel vThermo Scientific

Table of Contents

Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Please Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

General Usage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Measuring Liquid Nitrogen Quantity . . . . . . . . . . . . . . . . . . . . . . . .4-1

General Cleaning Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

Storing Samples in Vapor Phase . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Liquid Nitrogen Level Monitor Operation . . . . . . . . . . . . . . . . . . . . . . . .6-1

Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Remote Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Alarm Contact Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Microprocessor Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4

Principles of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4

Level Monitor Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

Safety Feature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

Audible and Visual Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

LN2 Level Monitor Troubleshooting and Parts List . . . . . . . . . . . . . . .7-1

Ordering Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2

Section 1

Section 2

Section 3

Section 4

Section 5

Section 6

Section 7

Cryogenic Storage Vessel 1-1Thermo Scientific

Please Read

Section 1 Safety Information

This manual contains important operating and safety information. The
user must carefully read and understand the contents of this manual prior
to the use of this equipment.

Your Thermo Scientific cryogenic vessel has been designed with function,
reliability, and safety in mind. It is the user’s responsibility to install it in
conformance with local electrical codes. For safe operation, please pay
attention to the warnings and cautions throughout the manual.

All cryopreservation vessels, regardless of who manufactures them, use a
vacuum to provide the super insulative properties needed to keep nitrogen
in a liquid form. They are very similar, in fact, to the Thermos® vessels you
may have used to store coffee, soup or milk.

Remember how fragile they were? Since no vacuum is perfect or will last
forever, we suggest that you monitor the consumption of liquid nitrogen
used by your vessel on a regular basis, i.e. every 1-2 days. By monitoring,
you may be able to anticipate subtle changes in consumption and possibly
react to problems before they arise. You may opt to use a level monitor for
this purpose (see page 6-1 for level monitor operation.).

Also, if your samples are super critical, one-of-a-kind, or irreplaceable,
consider allocating your samples to 2 separate vessels. Remember the old
adage about placing all your eggs into one basket….

Finally, because no vacuum will last forever, have your vessel tested by the
manufacturer or a qualified cryovessel service technician every 7-10 years,
regardless of any problems (or lack thereof) you may have had in the past.
This will help insure your samples against sudden loss of nitrogen due to
vacuum failure.

Do not use this product for anything other than its intended usage.

Use of the equipment in a manner not specified by the manufacturer may
impair the protection provided by the equipment.

General Usage

1-2 Cryogenic Storage Vessel Thermo Scientific

Section 1

Safety Information

• This unit is intended to be used by trained personnel in a well­ventilated environment.

• Liquid nitrogen is extremely cold; it boils at -196°C.

• Follow all necessary precautions and warnings relevant to applicable
hazards such as frostbite, contact burn, asphyxiation, etc. in cryogenic
area.

• To avoid injury due to frostbite or ruptured vials, use extreme care
whenever handling liquid nitrogen, liquid nitrogen storage or transfer
vessels, or any objects which have come in contact with liquid
nitrogen.

• Leave no areas of skin exposed.

• Wear appropriate PPE where necessary; face shield, cryogenic gloves,
cryogenic apron.

• Do not tightly seal liquid nitrogen containers or prevent nitrogen gas
from escaping.

• Always handle ampules with tongs. They may explode when removed
from the vessel.

• Use extreme care to prevent spilling and splashing liquid nitrogen
during transfer and removal of storage contents and holders.

• Immediately remove any clothing or safety attire on which liquid
nitrogen has been spilled or splashed.

• Get immediate medical attention for any frostbite injuries due to
liquid nitrogen.

Warnings