Thermo Fisher Scientific Qubit 4 Reference Guide
QUICK REFERENCEQUICK REFERENCE Qubit™ Assays
Standards from kit
Ensure all reagents are
at room temperature
Qubit
QUICK REFERENCE
Qubit™ Assays
Pub. No. M AN0 017210
Rev. C.0
* where n = number of standards plus number of samples
For Researc h Use Only. Not for u se in diagnost ic procedure s.
General Qubit Assay Protocol
1. Set up two assay tubes for the standards (three for
the protein or RNA IQ assay) and one assay tube for
each sample.
2. Prepare the Qubit™ working solution by diluting the
Qubit™ reagent 1:200 in Qubit™ buer. Prepare 200 μL of
working solution for each standard and sample.
3. Prepare the assay tubes* according to the table below.
†
™
reagent
Qubit
buffer
™
1 × n μL*
199 × n μL*
™
Qubit
working solution
190 μL
180-199 μL
10 μL 10 μL
™
Final Qubit
User samples
1-20 μL 1-20 μL 1-20 μL
Final Qubit™ volume tube is 200 μL
4. Vortex all tubes for 2–3 seconds.
5. Incubate the tubes for 2 minutes at room temperature
(15 minutes for the Qubit™ protein assay).
6. Insert the tubes in the Qubit™ Fluorometer and take
readings. For detailed instructions, refer to the Qubit
Fluorometer manual.
tube volume is 200 μL
™
†
Working solution
Standard (from kit)
User sample — 1–20 μL
Total Volume in each assay tube 200 μL 200 μL
†
Qubit 1X dsDNA assays (Cat. Nos. Q33230, Q33231, Q33265, Q33266) are supplied with a ready-to-use work ing solution, and do not require preparation.
* Use only thin-wall, clear 0.5 mL PCR tubes. Acceptable tubes include Qubit
(from step 2) 190 μL 180 –19 9 μ L
™
assay tubes (set of 50 0, Cat. No. Q32856).
Standard assay tubes User sample assay tubes
10 μL —
For Qubit Protein BR Assay only
1. Set up two assay tubes for the standards, one assay tube
for a buer blank and one assay tube for each sample.
2. Prepare assay tubes according to the table below. Adding
3. Vortex for 5-7 seconds immediately after addition of
Protein BR Assay Reagent.
4. Incubate tubes for 10 minutes at room temperature.
solutions in the following order to the assay tubes: (1)
Standards/Sample/Sample Buer, (2) Protein BR Assay
Buer, (3) Protein BR Assay Reagent.
5. Insert the tubes in the Qubit™ Fluorometer and take
readings. For detailed instructions, refer to the Qubit
Fluorometer manual.
Standard assay tubes Buffer blank User sample assay tubes
Standard (from kit) 20 µL - -
User sample buffer
User sample - - 10 or 20 µL
Protein BR Assay Buf fer 150 µL 150 or 160 µL 150 or 160 µL
Protein BR Assay Reagent 30 µL 30 µL 30 µL
Total volume in each assay tube 200 µL 200 µL 200 µL
Add 150 or 160 µL
Add 10 or 20 µL Mix & vortex for 5 sec
- 10 or 20 µL -
Add 30 µL
™
STD 1 STD 2 Sample Blank
Protein BR
Assay Buffer
Step 1: Prepare
assay tubes
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YOUR USE OF IT.
Step 2: Add Protein BR
Assay Buffer
Step 3: Add Protein
BR Reagent
Protein BR
Reagent
Final volume is 200 µL
Step 4: Incubate for
10 min at RT
Impor tant Li censin g Inform ation: T hese pro ducts may b e covere d by one or mo re Limite d Use Lab el
Licen ses. By us e of these p roducts , you acce pt the term s and con dition s of all app licabl e Limite d Use
Label Licenses.
Manufacturer: Multiple Life Technologies Corporation manufacturing sites are responsible for
manufacturing the products associated with the workflow covered in this guide.
Step 5:
Read samples
Find out more at thermofisher.com/qubit
For Research Use Only. Not for use in diagnostic procedures. © 2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are
the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. 12 March 2021
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