QuantStudio™ 6 Pro Real-Time PCR System
and QuantStudio™ 7 Pro Real-Time PCR
System
USER GUIDE
Publication Number MAN0018045
Revision G.0
For Research Use Only. Not for use in diagnostic procedures.
Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: T
O THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0018045
RevisionDateDescription
G.017 February 2021v1.4.0
Updat
ed centrifuges for the TaqMan™ Array Card.
F.06 November 2020v1.4.0
•
Specified that the instrument and the desktop software must be connected to the same SAE server
for an account password to be updated on both applications.
•
Provided the option of a static IP address or a dynamic IP address to connect to the SAE server.
•
Corrected standards on 384-well RNase P plate (Figure 14 on page 105).
•
Added a TaqMan™ Array Card block, including system template file names, calibration workflow,
instructions to edit the plate file, and instructions to load and unload from the instrument
(QuantStudio™ 7 Pro System only).
•
Added instructions to review the calibration status in the QuantStudio™ Design and Analysis
Software v2.
•
Added a peripheral self-test and updated the previous self-verification test to a hardware test.
•
Added instructions to upload a certificate for a TaqMan™ Array Plate with RFID.
•
Added the option of a voice recording for Smart Help.
•
Added remote support.
•
Updated the RFID labels (Figure 27 on page 215 and Figure 28 on page 216).
E.027 April 2020v1.3.0
•
•
•
•
•
•
•
•
•
D.07 January 2020v1.2.1
•
•
•
•
•
C.03 December 2019v1.2.0
•
•
•
•
Added protein thermal shift experiments to the instrument functions.
Added a 96-well 0.1-mL block.
Updated instructions to export data files when setting up a run and added instructions to import or
delete export settings.
Updated instructions to save an edited run method.
Updated instructions to add or edit reagents and then to apply the reagents to the plate.
Updated instructions to view the amplification plot after a run.
Added instructions to view QC Check status after the run.
Added information about a locked template file.
Added functions for using the instrument with the SAE Administrator Console.
Added information about position to set up facial authentication and to sign in with facial
authentication.
Added instructions to test the connection to the Connect cloud-based platform and to view the
privacy policy for the Connect platform.
Added instructions to repair the software after a software update.
Changed port 7000 to 7443 for the instrument-to-computer connection.
Corrected Ct to Cq in description of instrument verification.
Added a 384-well plate block.
Updated instructions to set up facial authentication.
Added a new voice command for calibration.
Added custom melt curve run calibration.
RevisionDateDescription
C.0
(continued)
B.022 August 2019v1.1.0
3 December 2019
•
Added Security, Auditing, and E‑signature (SAE) v2.0 module.
•
Added instructions to generate and reset an access key for external software to connect to the
instrument.
•
Added instructions to import an Assay Information File.
•
Corrected that a Connect profile is required for the instrument schedule and Smart Help.
•
Added new radio compliance standards.
•
Corrected the text on the buttons for the following items:
–
Add facial authentication to an existing instrument profile.
–
View the license agreement.
–
Reset the instrument (restore factory default).
•
Added the following items:
–
TET™ dye as a system dye.
–
Information about sleep mode.
–
Information about the automatic sign-out feature.
–
The option to configure the instrument so that sign-in is required (guest access is not
permitted).
–
Instructions to set up email notification.
–
Information about the speaker volume for each user.
–
Instructions to back up the instrument and to restore a backup of the instrument.
–
Information about multiple instruments and email notification in the comparison of a local
instrument profile and a Connect profile.
–
Instructions to unlink a Connect profile.
–
Instructions to update a PIN for a Connect profile.
–
Lighting guidelines for facial authentication.
–
Radio compliance standards and RF transceiver specifications.
•
Corrected the following items:
–
Indicators for voice commands.
–
Voice commands are only available in select countries.
–
Instructions to remove VeriFlex™ Zones and the Auto Delta setting.
–
Excitation and emission wavelengths of the filters in the calibration instructions.
–
Add a four-digit PIN when signing into the instrument for the first time using a Connect
profile.
–
Add a four-digit PIN when linking a local instrument profile to a Connect profile.
–
Select a region for the Connect cloud-based platform.
–
A run cannot be started if a block has never been calibrated on the instrument.
–
The options after a calibration run is complete.
–
The example files for the desktop software are embedded in the software and do not need to
be downloaded.
•
Updated the following items:
–
Cq Export checkbox is selected as the default.
–
Instructions to sign in with facial authentication.
A.018 April 2019New document (v1.0.1).
Important Licensing Information: These pr
oducts may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
NOTICE TO PURCHASER: DISCLAIMER OF LICENSE: Purchase of this software product alone does not imply any license under any
process, instrument or other apparatus, system, composition, reagent or kit rights under patent claims owned or otherwise controlled by
Thermo Fisher Scientific, either expressly, or by estoppel.
Trademarks: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is
a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Cy is a registered trademark of GE
Healthcare. Amazon, Alexa, and all related logos are trademarks of Amazon.com, Inc. or its aliates. Windows is a trademark of
Microsoft Corporation. Chrome is a trademark of Google LLC. Pentium is a trademark of Intel Corporation or its subsidiaries in the U.S.
and/or other countries. Macintosh is a trademark of Apple Inc., registered in the U.S. and other countries.
The network configuration and security settings of your laboratory or facility (such as firewalls, anti-
virus software, network passwords) are the sole responsibility of your facility administrator, IT, and
security personnel. This product does not provide any network or security configurationfiles, utilities, or
instructions.
If external or network drives are connected to the software, it is the responsibility of your IT personnel
to ensure that such drives are configured and secured correctly to prevent data corruption or loss. It
is the responsibility of your facility administrator, IT, and security personnel to prevent the use of any
unsecured ports (such as USB, Ethernet) and ensure that the system security is maintained.
Password security
Thermo Fisher Scientific strongly recommends that you maintain unique passwords for all accounts in
use on this product. All passwords should be reset upon first sign in to the product. Change passwords
according to your organization's password policy.
It is the sole responsibility of your IT personnel to develop and enforce secure use of passwords.
14
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
Instrument hardware description
Overview of the instrument
The Applied Biosystems™ QuantStudio™ 6 Pro and 7 Pro Real-Time PCR Systems use fluorescentbased polymerase chain reaction (PCR) reagents to perform the following functions:
•
Quantitative detection of target nucleic acid sequences (targets)
•
Qualitative detection of targets (endpoint analysis)
•
Qualitative analysis of the PCR product (post-PCR target nucleic acid sequences melt curve
analysis)
•
Protein thermal shift experiments
Chapter 1 Product information
Instrument hardware description
1
Each instrument is available with an interchangeable block. The following blocks are available:
•
96-well 0.2-mL block
•
96-well 0.1-mL block
•
384-well block
•
TaqMan™ Array Card block (QuantStudio™ 7 Pro System only)
The instrument can be used directly from the touchscreen to create and start runs. To design runs or to
analyze data, the instrument can be integrated with the desktop software or the Connect platform.
Each instrument has the following features:
•
Sign-in with facial authentication
•
An interchangeable block
•
Microphone for voice commands
•
Speakers for feedback from voice commands and for instructional videos
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
15
Chapter 1 P
1
Instrument hardware description
•
•
roduct information
Smart Help to request technical support or instrument service directly from the instrument
Barcode scanner for tracking plates or TaqMan™ Array Cards
Features of each instrument
Feature
Filter set
[1]
VeriFlex™ Zones
TaqMan™ Array Card
block
Security, Auditing, and
E‑signature (SAE) v2.0
module
[1]
S
ee “System dyes” on page 16.
[2]
See “VeriFlex™ Zones” on page 18.
[3]
See Chapter 9, “Use the instrument with the Security, Auditing, and E‑signature (SAE) v2.0 module”.
Instrument filt
System dyes
The QuantStudio™ 6 Pro Real-Time PCR System has a coupled five-colorfilter set.
The QuantStudio™ 7 Pro Real-Time PCR System has a decoupled six-by-six color filter set.
For more information about available spectral dye calibration kits, contact Support.
QuantStudio™ 6 Pro SystemQuantStudio™ 7 Pro System
Coupled five-colorfilter setDecoupled six-by-six color filter set
he central wavelengths are the optimized wavelengths.
[2]
The HEX™ dye and TET™ dye from Thermo Fisher Scientific fall within the emission wavelength range of the system, therefore they
can be added and adapted for use on the instrument.
[3]
This filter set currently does not support any dyes supplied by Thermo Fisher Scientific.
MUSTANG PURPLE
dye, Cy®5
[3]
™
LIZ™ dye
Cy®5.5 dye
[2]
16
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
Filters
Wavelength
(nm)
1 2
3
4
5
x1-m1 x2-m2
x3-m3
x4-m4
x5-m5 x6-m6
Emission
Spectra
Chapter 1 P
Instrument hardware description
roduct information
1
x1-m1—FAM™ dye, S
1
x2-m2—VIC™ dye
2
x3-m3—ABY™ dye, NED™ dye, Cy®3, T
3
YBR GREEN™ dye
AMRA™ dye
x4-m4—JUN™ dye, RO
4
x5-m5—Cy® 5, MUS
5
X™ dye, Texas Red™ dye
TANG PURPLE™ dye
Custom dyes
The instrument can run assays designed with custom dyes. Custom dyes include the following options:
•
Dyes that are not supplied by Thermo Fisher Scientific.
•
Dyes or formulations of dyes that are not system dyes for the instrument.
To use a custom dye on the instrument, review the following requirements.
•
Calibrate the instrument for the custom dye (see “Calibrate custom dyes” on page 113).
•
Ensure that the custom dye excites between 455–672 nm and emits between 505–723 nm.
•
Select a custom dye that does not overlap with other dyes used in the run (see “System dyes” on
page 16).
•
The custom dye for use in calibration must be attached to the 5' end of a short DNA
oligonucleotide consisting of the first two bases of the probe sequence without a quencher at
the 3' end.
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
17
Chapter 1 Product information
1
Instrument hardware description
Overview of data collection
The instrument collects raw fluorescence data at dierent points during the PCR cycle, depending on
the type of run performed.
When you create a plate file, you can customize the optical filter channels through which the instrument
collects data. You can specify a filter channel set for all PCR thermal protocols and for melt curve
stages.
Run typeRun typeData collection point
•
Real-time
Standard curve
•
Relative standard curve
•
Comparative Ct (DDCt)
•
Melt curve
During the thermal cycling protocol.
Typical timing is to collect data at each cycle of a PCR
stage or continuously during a melt stage.
Post-PCR
(endpoint)
VeriFlex™ Zones
VeriFlex™ Z
control over your real-time PCR runs.
The independent zones are ideal for real-time PCR optimization. They also provide the ability to perform
multiple runs at the same time. Unlike standard gradients which give a sigmoidal temperature curve
across the columns, blocks with VeriFlex™ Zones help deliver accurate temperatures across every zone.
The QuantStudio™ 6 Pro System has three VeriFlex™ Zones (Figure 1 on page 19). The QuantStudio™ 7
Pro System has six VeriFlex™ Zones (Figure 2 on page 19).
Note: VeriFlex™ Zones are only applicable for the 96-well 0.2-mL block and the 96-well 0.1-mL block.
ones provide independent temperature zones that oer enhanced functionality and precise
•
Genotyping
•
Presence/absence
•
After thermal cycling is completed.
For Presence/Absence and Genotyping analysis,
data collection before the PCR cycle is optional, but
recommended.
•
(Optional) During the thermal cycling protocol.
Collecting data during the run can confirm
genotyping results by viewing traces in allelic
discrimination plots or viewing genotyping calls at
earlier cycles.
18
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
Chapter 1 P
Instrument hardware description
roduct information
1
Figure 1 Three VeriFlex™ Z
Figure 2 Six VeriFlex™ Z
Note: Plate files are not compatible between the QuantStudio™ 6 Pro System and the QuantStudio™ 7
Pro System. For more information, see “Compatibility of plate files” on page 29.
AutoDelta settings
AutoDelta enables an incremental increase or decrease in the temperature or time in a PCR cycle.
o use AutoDelta, specify a dierence in the time and the temperature. Specify the PCR cycle for this
T
change to be applied.
ones
ones
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
19
1
2
3
4
5
6
8
9
7
Chapter 1 Product information
1
Instrument hardware description
Parts of the instrument
Figure 3 Front of the instrument
Cameras
1
Touchscreen
2
Microphones
3
USB port
4
Indicator light
5
Proximity sensor
6
Access door for block change
7
Speakers
8
Drawer
9
20
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
2
3
4
6
5
1
Chapter 1 P
Instrument hardware description
roduct information
1
Figure 4 Back of the instrument
USB ports
1
Wi-Fi dongle port
2
Ethernet port
3
Note: The instrument r
The USB ports at the rear of the instrument can be used for external devices, for example, a mouse or a
k
eyboard.
ecognizes only one external storage device at a time for data transfer.
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
RS-232 port (service use only)
4
ON/OFF switch
5
Power inlet receptacle
6
21
Chapter 1 Product information
1
Hands-free features
Instrument status indicator
Indicator
OPowered o
Blue light changing brightness slowlyPowering on
Blue light changing brightnessStandby
Blue light moving inwards towards middle of
instrument
Blue light moving outwards towards side of
instrument
Blue light moving back and forthBlock is being changed
Blue light onInstrument is ready to start a run or is performing a
Amber blinkingError
Hands-free features
Instrument status
Sleep mode
Drawer opening
Drawer closing
run
Facial authentication
The instrument allows hands-free facial authentication to sign in a user. The instrument camera detects
a face. The instrument will sign in the user after the face is matched to the photo associated with a
profile.
Voice commands
The instrument recognizes a set of commands for hands-free operation. For list of supported voice
commands, see “Use voice commands” on page 45.
Voice commands are only available when a user is signed in with a Connect account. Voice commands
are not available with a local instrument profile.
Voice commands are only available in select countries.
Voice commands use Amazon™ Alexa™ for Business.
22
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
Indicators for the hands-free features
Indicators for facial authentication
Chapter 1 Product information
Hands-free features
1
Display
The Sign In screen displays .Facial authentication for the instrument is enabled.
•
The Sign In screen does not
display .
Facial authentication for the instrument is disabled.
•
All users must sign in with a PIN.
Note: To enable facial authentication, see “Configure the instrument
for the hands-free features” on page 185.
Status of facial authentication
Indicators for voice commands
For the location of the proximity sensor, see Figure 3 on page 20.
DisplayStatus of voice commands
(Gray)
(Gray)
The voice command function is not available because the proximity
sensor does not detect a person.
The voice command function is not available if any of these conditions
is true:
•
The voice command function for the instrument is disabled by an
administrator.
•
The user is signed in with a local profile.
•
The microphone was disabled by the user and the proximity
sensor does not detect a person. The icon will return to a red
state when the proximity sensor detects a person.
The instrument is preparing to accept voice commands.
(Amber)
(Blue)
(Red)
Note: The amber display is an intermediate state and will only appear
for several seconds.
The voice command function is available if all of these conditions are
true:
•
The voice command function for the instrument is enabled by an
administrator.
•
The user is signed in with a Connect instrument profile.
•
The proximity sensor detects a person.
The microphone was disabled by the user (see “Enable or disable the
microphone” on page 44).
To enable voice commands, see “Configure the instrument for the hands-free features” on page 185.
For more information about linking a local instrument profile to a Connect profile, see “Link the
instrument to your Connect account” on page 126 and “If you link when you are signed in to the
instrument” on page 129.
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
23
12
3
4
5
6
7
8
910
11
Chapter 1 Product information
1
Software description
Software description
Parts of the home screen
Figure 5 Home screen
Instrument name
1
Instrument calendar
2
Microphone
3
Help
4
Eject
5
Status dial
6
Table 1 Parts of the home screen
Element of the home
scr
een
Block installed
7
Current user
8
Connectivity icons
9
Buttons to access plate files
10
Instrument settings
11
Function
Instrument nameSet by the administrator to identify the instrument.
CalendarSchedule time for a run or see runs that are scheduled.
MicrophoneShows the status of the microphone (see “Indicators for voice commands” on
page 23 and “Enable or disable the microphone” on page 44).
HelpAccess the Help.
EjectOpen or close the drawer to insert or remove a plate or a TaqMan™ Array Card.
24
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
Table 1 Parts of the home screen (continued)
Chapter 1 P
roduct information
Software description
1
Element of the home
screen
Status dial
•
When the instrument is not in use—Displays Set up run.
•
When the instrument is in use—Displays the sample block temperature, the
Function
elapsed run time, and the run status.
•
When the instrument is locked while in use—Displays Locked within the status
dial.
•
When a run is complete—Displays Run complete.
Block typeThe block that is installed on the instrument.
If applicable, the following items are noted:
•
If there is no block and heated cover set installed.
•
If the block and heated cover are mismatched.
Current userDisplays the current signed-in user.
Guest is displayed if no user is signed in.
ConnectivityOnly the icons that apply to the connectivity status of the instrument at the time are
displayed.
•
—The instrument is wirelessly connected.
•
—The instrument is connected to a wired network.
•
—A USB drive is plugged into the instrument.
•
—The instrument is connected to a network drive.
•
—The instrument is connected to a Connect account.
Buttons to access plate
[1]
files
Access a plate file to edit it or start a run.
•
Set up run—Open a system template or a saved plate file.
•
(Load plate file)—Open a system template or a saved plate file.
•
(Run last)—Open the last plate file that was used to start a run. The last plate
file is specific to the profile.
•
Instrument settings
[1]
P
late files are applicable to TaqMan™ Array Cards.
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
Edit the file name convention.
•
View the run history, and transfer or delete data files.
•
Install, change, or remove the block and the heated cover.
•
Configure the instrument settings.
•
Configure the maintenance and service settings.
•
View notifications.
•
Configure hands-free operation.
25
Chapter 1 Product information
1
Software description
Connect and Cloud definitions
The terms Connect, Cloud, Thermo Fisher Connect, and Thermo Fisher Cloud are used interchangeably
on the instrument touchscreen. The term Connect is used in this document.
The following icons are used interchangeably on the instrument touchscreen:
•
•
The icon is used in this document.
Instrument, desktop software, and Connect platform features
The instrument and the software include the features described below.
Note: System templates cannot be edited. They can be edited, then saved as a separate plate file.
FeatureInstrumentDesktopConnect
Use as guest (no sign in)
Schedule a run
Open system templates
Open system templates, edit them, and save
them as plate files
Open plate files created on the desktop software
Open plate files created on the Connect platform
Open plate file created on the instrument
Send plate files to the instrument run queue—
Access the plate file associated with last run on
the instrument
Load plate in instrument
Start run
View real-time data during a run
View results after a run is complete
—
—
—
——
——
——
—
26
See instrument status (run in progress)
Send email notifications
Check calibration status
Analyze and export results
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
(Limited)
—
——
Chapter 1 Product information
Software description
(continued)
FeatureInstrumentDesktopConnect
View analysis of results—
1
Set calibration reminders
Review exported calibration or RNase P
verification results
—
Overview of file locations and files
Note: The term plate file is applicable to both plates and TaqMan™ Array Cards.
File locations
File location
Run queuePlate files sent from the desktop software.
Public
Templates
Description and function
•
Plate files saved by guest users are stored in this folder.
•
Data files from runs started by guest users are stored in this folder.
•
System templates are stored in this folder.
•
System templates cannot be edited. They must be saved as a separate plate
file and stored in a dierent folder.
•
Data files cannot be saved in this folder.
—
(RNase P only)
Connect
Network drive
USB drive
My instrument
•
Plate files can be retrieved from a Connect profile.
•
Data files can be saved to a Connect profile.
•
You must have a Connect profile.
•
Plate files can be retrieved from a network drive.
•
Data files can be saved to a network drive.
•
The instrument must be connected to a network or a computer.
•
Plate files can be retrieved from a USB drive.
•
Data files can be saved to a USB drive.
•
A USB drive must be inserted into the instrument.
•
This location is only available to a user who is signed in. Guest users cannot
access this location to retrieve plate files or save data files.
•
This location is specific to a user. Files are not accessible to any other users,
except for administrators.
•
Plate files can be retrieved from this location.
•
Data files are always saved to this location.
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
27
Chapter 1 Product information
1
Software description
(continued)
File location
Post-read
Description and function
•
In endpoint PCR, the pre-read data is saved in this folder for post-read
analysis.
Files
IMPORTANT! Plate files and data files are not included in a back-up of the instrument and are not
restored when a backup of the instrument is restored.
Plate files and data files must be transferred separately. See Chapter 6, “View and manage files”.
System templates are included in the back-up and are restored when a backup of the instrument is
restored.
All file types ar
ItemDescription and functionSupported in
System template
e applicable to both plates and TaqMan™ Array Cards.
•
A non-editable t
•
Accessed from
(only system templates are stored in this folder).
•
Accessed from the Set up run button.
•
Is saved as a separate plate file if it is edited.
•
Contains the thermal protocol for the real-time PCR.
•
Does not contain the sample information.
•
Does not contain the target information.
•
Sample information and target information must be added
(saved as a separate plate file). The sample information and
target information can be added on the desktop software or
on the Connect platform after the run is complete.
emplate.
(Load plate file)and the T
emplates folder
Instrument
Desktop
Connect
Plate File
From
28
(Load plate file)
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
•
A file that has been saved fr
•
Can be edited and saved.
•
Can be edited and saved as a separate plate file.
•
Contains the thermal protocol for the real-time PCR.
•
Can contain the sample information.
•
Can contain the target information.
•
Sample information and target information must be added if
not present. The sample information and target information
can be added on the desktop software or on the Connect
platform after the run is complete.
om a system template.
Instrument
Desktop
Connect
Chapter 1 P
roduct information
Software description
(continued)
ItemDescription and functionSupported in
•
Plate File
From (Run last)
The plate file associated with the last run for the profile.
•
Can be edited and saved.
•
Can be edited and saved as a separate plate file.
•
Contains the thermal protocol for the real-time PCR.
•
Contains the sample information. The sample information can
be edited on the desktop software or on the Connect platform
after the run is complete.
•
Contains the target information. The target information can be
edited on the desktop software or on the Connect platform
after the run is complete.
•
Sample information and target information can be edited on
the instrument.
•
If the sample information and the target information for the last
run were added on the desktop software or on Connect after
the run was complete, they will not be included on the plate
file that is on the instrument.
Instrument
1
Data file
Contains the r
•
Can be viewed on the instrument only immediately after the
run is complete.
•
Transfer to Connect or to the desktop software to view and
analyze data.
eal-time PCR data.
•
Compatibility of plate files
The plat
A plate file for a QuantStudio™ 6 Pro System can only be opened on this instrument. It cannot be
opened on a QuantStudio™ 7 Pro System.
A plate file for a QuantStudio™ 7 Pro System can only be opened on this instrument. It cannot be
opened on a QuantStudio™ 6 Pro System.
e files are specific to the instrument.
Instrument (limited)
Desktop
Connect
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
29
Chapter 1 Product information
1
Software description
Components of the files
Component
Properties
MethodThermal cycling conditions
InstrumentDesktopConnect
•
Data file name
•
Reagent information
–
Name
–
Type
–
Lot number
–
Barcode
–
Catalog number
–
Expiry date
•
Plate barcode or TaqMan
Array Card barcode
•
Location to send data after the
run is complete
•
T
emperature
•
Time
•
Cover temperature
•
Reaction volume
•
Data collection points
•
Ramp rates
•
VeriFlex™ Zones
•
Auto Delta settings
•
Experiment file name
•
Plate barcode or TaqMan™ Array Card barcode
•
User name
•
Instrument type
•
Block type
•
Analysis module
•
Chemistry (reagent information)
•
Run mode
™
•
Comments
•
(Connect platform only) Notifications
Thermal cycling conditions
PlateSample names
Targets
SNP assays
RunMonitor a run in progress
View time remaining, temperature,
method, and amplification plots
Pause, resume, or stop a run
ResultsNot applicableReview plots
ExportExport results
Data files are transferred to the
desktop software or the Connect
platform for analysis
30
QuantStudio™ 6 Pro Real-Time PCR System and QuantStudio™ 7 Pro Real-Time PCR System User Guide
Define and assign samples, targets or SNP assays, and
tasks in the Plate setup screen.
Monitor a run in progress
View time remaining
Export results
Monitor a run in progress
(link to Connect InstrumentDetails)
View time remaining,
temperature, method, and
amplification plots
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