Thermo Fisher Scientific ProcartaPlex User Manual
ProcartaPlex™ Human Brain Injury Panel 4Plex
USER GUIDE
Catalog Number EPX040-15827-901
Publication Number MAN0019769
Revision A.0 (30)
For Research Use Only. Not for use in diagnostic procedures.
Bender MedSystems GmbH | Campus Vienna Biocenter 2 | 1030 Vienna, Austria
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0019769
Revision Date Description
A.0 6 January 2021 New manual
End User License Agreement for Luminex Assay Products: By opening the packaging containing this Assay Product (which contains
fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are
consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at
https://www.luminexcorp.com/end-user-terms-and-conditions/#sec3. If you do not agree to all of the terms and conditions, you must
promptly return this Assay Product for a full refund prior to using it in any manner.
TRADEMARKS: xMAP, Luminex 100/200, MAGPIX, INTELLIFLEX and FLEXMAP 3D are trademarks of Luminex Corp. and Bio-Plex is a
trademark of Bio-Rad Laboratories Inc. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise
specified.
©2021 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■
CHAPTER 1 ProcartaPlex™ Human Brain Injury Panel 4-Plex .................. 4
Introduction .................................................................... 4
Contents and storage ............................................................ 5
Required materials not supplied ................................................... 5
Precautions and technical hints ................................................... 6
Workflow ....................................................................... 7
■
CHAPTER 2 Methods ............................................................... 8
Sample preparation ............................................................. 8
Plasma sample preparation .................................................. 8
Serum sample preparation ................................................... 8
CSF sample preparation ..................................................... 8
Preparation of reagents .......................................................... 8
Prepare 1X Wash Buer ..................................................... 9
Prepare Standard Mix ....................................................... 9
Prepare 4-fold serial dilution .................................................. 9
Assay protocol ................................................................. 11
Instrument settings ............................................................. 13
Analyze results ................................................................ 13
■
APPENDIX A Recommended plate layout ...................................... 14
ProcartaPlex
™
Human Brain Injury Panel 4-Plex
3
1
WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear
appropriate protective eyewear, clothing, and gloves. Safety Data Sheets (SDSs) are available from
thermofisher.com/support.
Introduction
The ProcartaPlex™ Human Brain Injury Panel 4-Plex has been optimized for detection of multiple
analytes from serum, plasma, and cerebrospinal fluid (CSF).
The panel is provided in a ready-to-use format with individual vials of 1X capture and detection
reagents that require less pipetting and experimental setup. These reagents are not combinable with
simplexes or other panels.
ProcartaPlex™ Human Brain Injury
Panel 4-Plex
ProcartaPlex™ preconfigured panels are extensively tested for analyte combinability, interference and
cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex™ panels are
supplied with the necessary reagents to perform the assay.
Analytes
GFAP NF-H S100B
UCHL1
For detailed product information, visit thermofisher.com/procartaplex
4
ProcartaPlex™Human Brain Injury Panel 4-Plex
Contents and storage
Upon receipt, store the kit at 2°C to 8°C. When stored as indicated, all reagents are stable until the
expiration date.
Contents Amount
Standard Mix 4 (lyophilized) 2 each
Biotinylated Detection Antibody Mix (1X) 1 x 3.5 mL
Capture Bead Mix (1X) 1 x 5 mL
Streptavidin-PE (SA-PE) (1X) 1 x 5 mL
Wash Buer (10X) 1 x 25 mL
Reading Buer (1X) 1 x 40 mL
Universal Assay Buer (1X) 1 x 10 mL
8-Tube Strip 2 each
Chapter 1
ProcartaPlex™ Human Brain Injury Panel 4-Plex
Contents and storage
1
Flat Bottom 96-well Plate, black 1 each
Microplate Lid 1 each
Plate Seals 8 each
Retain the lot-specific Certificate of Analysis that contains the product expiration date. The Certificate
of Analysis also contains important information such as bead number, analyte names and highest
standard concentration required for the assay setup on the xMAP instrument.
CAUTION! This kit contains materials with small quantities of sodium azide. Sodium azide reacts
with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a
large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin and
mucous membranes. In case of contact, rinse aected area with plenty of water. Observe all federal,
state and local regulations for disposal.
Required materials not supplied
• xMAP™ instrument
• Hand-Held Magnetic Plate Washer (Cat. No. EPX-55555-000)
• Deionized water
• Vortex mixer (e.g., Cat. No. 88882010)
• Microcentrifuge
• Adjustable single and multichannel pipettes with disposable tips and low volume reservoirs (e.g.,
Cat. No. 95128093)
ProcartaPlex™Human Brain Injury Panel 4-Plex
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