Thermo Fisher Scientific Platinum ProcartaPlex User Manual
Platinum ProcartaPlex™ Human Panel 1 42Plex
USER GUIDE
Catalog Number EPXP420-10200-901
Publication Number MAN0024733
Revision A.0 (30)
For Research Use Only. Not for use in diagnostic procedures.
Bender MedSystems GmbH | Campus Vienna Biocenter 2 | 1030 Vienna, Austria
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The information in this guide is subject to change without notice.
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LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
End User License Agreement for Luminex Assay Products: By opening the packaging containing this Assay Product (which contains
fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are
consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at
https://www.luminexcorp.com/end-user-terms-and-conditions/#sec3. If you do not agree to all of the terms and conditions, you must
promptly return this Assay Product for a full refund prior to using it in any manner.
Revision history: Pub. No. MAN0024733
Revision Date Description
A.0 (30) 15 February 2021 New manual
TRADEMARKS: xMAP, Luminex 100/200, MAGPIX, INTELLIFLEX and FLEXMAP 3D are trademarks of Luminex Corp. and Bio-Plex is a
trademark of Bio-Rad Laboratories Inc. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise
specified.
©2021 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■
CHAPTER 1 Platinum ProcartaPlex™ Human Panel 1 42-Plex .................. 4
Introduction .................................................................... 4
Contents and storage ............................................................ 5
Required materials not supplied ................................................... 6
Precautions and technical hints ................................................... 6
Workflow ....................................................................... 7
■
CHAPTER 2 Methods ............................................................... 8
Sample preparation ............................................................. 8
Plasma sample preparation .................................................. 8
Serum sample preparation ................................................... 8
Dilution of plasma and serum samples ........................................ 8
Preparation of reagents .......................................................... 8
Prepare 1X Wash Buer ..................................................... 9
Prepare Standard Mix, High Control, and Low Control .......................... 9
Prepare 4-fold serial dilution .................................................. 9
Expected values of controls ................................................. 10
Assay protocol ................................................................. 11
Performance characteristic: Spike and dilution recovery ............................. 13
Instrument settings ............................................................. 15
Analyze results ................................................................ 15
■
APPENDIX A Recommended plate layout ...................................... 16
Platinum ProcartaPlex
™
Human Panel 1 42-Plex User Guide
3
1
Introduction
The Platinum ProcartaPlex™ Human Panel 1 42-Plex has been optimized for detection of multiple
analytes from serum and plasma. This panel assures high performance specifications (e.g., spike and
dilution recovery results of 70–130%) by using matrix type specific diluents for plasma and serum that
help to overcome matrix eects.
The panel is provided in a ready-to-use format with individual vials of 1X capture and detection
reagents that require less pipetting and experimental setup. These reagents are not combinable with
simplexes or other panels.
ProcartaPlex™ preconfigured panels are extensively tested for analyte combinability, interference and
cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex™ panels are
supplied with the necessary reagents to perform the assay.
Platinum ProcartaPlex™ Human
Panel 1 42-Plex
Analytes
BDNF Eotaxin GM-CSF
Gro alpha HGF IFN-alpha
IFN-gamma IL-1 alpha IL-1 beta
IL-1RA IL-2 IL-4
IL-5 IL-6 IL-7
IL-8 IL-9 IL-10
IL-12p70 IL-13 IL-15
IL-16 IL-17A IL-20
IL-21 IP-10 LIF
MCP-2 MIP-1 alpha MIP-1 beta
OPG PDGF-BB PECAM-1
P-Selectin RANTES SCF
4
Platinum ProcartaPlex™ Human Panel 1 42-Plex User Guide
(continued)
TNF alpha TNF-R2 tPA
TSLP VEGF-A VEGF-D
Contents and storage
Upon receipt, store the kit at 2°C to 8°C. When stored as indicated, all reagents are stable until the
expiration date.
Contents Amount
Platinum Standard Mix (lyophilized) 2 each
Platinum High Control 2 each
Platinum Low Control 2 each
Chapter 1 Platinum ProcartaPlex™ Human Panel 1 42-Plex
Contents and storage
Analytes
1
Biotinylated Detection Antibody Mix (1X) 1 x 3.5 mL
Capture Bead Mix (1X) 1 x 5 mL
Streptavidin-PE (SA-PE) (1X) 1 x 5 mL
Wash Buer (10X) 1 x 25 mL
Reading Buer (1X) 1 x 40 mL
Serum Assay Diluent 1 x 10 mL
Plasma Assay Diluent 1 x 10 mL
8-Tube Strip 2 each
Flat Bottom 96-well Plate, black 1 each
Microplate Lid 1 each
Plate Seals 8 each
Retain the lot-specific Certificate of Analysis that contains the product expiration date. The Certificate
of Analysis also contains important information such as bead number, analyte names and highest
standard concentration required for the assay setup on the xMAP instrument.
CAUTION! This kit contains materials with small quantities of sodium azide. Sodium azide reacts
with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a
large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin and
mucous membranes. In case of contact, rinse aected area with plenty of water. Observe all federal,
state and local regulations for disposal.
Platinum ProcartaPlex™ Human Panel 1 42-Plex User Guide
5
Chapter 1 Platinum ProcartaPlex™ Human Panel 1 42-Plex
1
Required materials not supplied
Required materials not supplied
• xMAP™ instrument
• Hand-Held Magnetic Plate Washer (Cat. No. EPX-55555-000)
• Deionized water
• Vortex mixer (e.g., Cat. No. 88882010)
• Microcentrifuge
• Adjustable single and multichannel pipettes with disposable tips and low volume reservoirs (e.g.,
Cat. No. 95128093)
• Beakers, flasks, and cylinders necessary for preparation of reagents
• Orbital microplate shaker with at least 1.5 mm or 0.059 inch orbit diameter capable of maintaining a
speed of 600 ± 50 rpm (e.g., Cat. No. 88882006)
Note: The use of rockers or large orbit shakers may cause adverse results.
Precautions and technical hints
1. Thoroughly read this User Guide and Certificate of Analysis prior to using the kit.
2. All chemicals should be considered potentially hazardous.
3. To avoid cross-contamination, do not invert the assay plate during the assay or allow contents
from one well to mix with another well.
4. Use a multichannel pipette and reagent reservoirs whenever possible to achieve optimal assay
precision.
5. Ensure that the xMAP™ instrument has been properly calibrated and set up prior to preparing and
running the assay.
6
Platinum ProcartaPlex™ Human Panel 1 42-Plex User Guide
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