Thermo Fisher Scientific pH 300, pH 310 User Manual

gyM

sy...

Instruction Manual

pH 300/310

Waterproof Hand-held pH / mV / Temperature Meter

Technolo

adeEa

68X248901

Rev 9 Jun 07

Part of Thermo Fisher Scientific

Preface

This manual serves to explain the use of the waterproof pH 300/310 hand-held meter. It

functions in two ways, firstly as a step by step guide to help you to operate the meter.

Secondly, it serves as a handy reference guide. It is written to cover as many anticipated

applications of the waterproof pH 300/310 meter as possible. If there are doubts in the use of

the meter, please do not hesitate to contact the nearest Authorized Distributor.

Eutech Instruments/ Oakton Instruments cannot accept any responsibility for damage or

malfunction to the meter caused by improper use of the instrument.

The information presented in this manual is subject to change without notice as improvements

are made, and does not represent a commitment on the part of Eutech Instruments Pte Ltd/

Oakton Instruments.

Eutech Instruments Pte Ltd/ Oakton Instruments

TABLE OF CONTENTS

1 INTRODUCTION 1 2 DISPLAY AND KEYPAD FUNCTIONS 2

2.1 Display 2

2.2 Keypad 3

2.3 Inserting the Batteries 4

2.4 Connecting the Electrode and Temperature Probe 5

2.5 Attaching the Electrode Holder to the Meter 7

2.6 Inserting the Electrode into the Electrode Holder 7

3 CALIBRATION 8

3.1 Important Information on Meter Calibration 8

3.2 Preparing the Meter for Calibration 8

3.3 pH Calibration 10

3.4 Relative mV Calibration 12

3.5 Temperature Calibration 13

4 MEASUREMENT 14

4.1 Automatic Temperature Compensation 14

4.2 Manual Temperature Compensation 15

4.3 Taking Measurements 16

5 HOLD FUNCTION 17 6 MEMORY FUNCTION 18

6.1 Memory Input 18

6.2 Memory Rec all 19

7 ADVANCED SETUP FUNCTIONS 21

7.1 Advanced SET-UP Mode Overview 23

7.2 P 1.0: Memory Clear (CLr) 25

7.3 P2.0: Viewing previous calibration data 26

7.4 P3.0: Viewing electrode data 27

7.5 P4.0: Unit configuration 29

7.6 P5.0: Resetting to factory default settin g s 34

7.7 P5.0: Setting the real-time clock 35

7.8 P6.0: Resetting to factory default settin g s 37

8 PROBE CARE AND MAINTENANCE 38 9 TROUBLE SHOOTING GUIDE 40 10 ERROR MESSAGES 41 11 SPECIFICATIONS 42 12 ACCESSORIES 43 13 ADDENDUM 1: METER FACTORY DEFAULT SETTINGS 46 14 WARRANTY 47 15 RETURN OF ITEMS 48

1 INTRODUCTION

Thank you for selecting this meter. The waterproof pH 300/310 Hand-held meter is a

microprocessor-based instrument that is designed to be handy and user-friendly. It is capable of

measuring pH, mV and temperature. It is designed to be handy and capable of allowing one-hand

operation. It is completely WATERPROOF --- and it FLOATS!

This meter has many user-friendly features – all of which are completely accessible through the

water-resistant membrane keypad. Your meter includes a temperature probe, two electrode holders,

batteries, instruction manual and a warranty card. Complete portable testing versions, including a

rugged carrying case and calibration solutions, are also available. Refer to the Accessories section

for more information.

Please read this manual thoroughly before operating your meter.

2 DISPLAY AND KEYPAD FUNCTIONS

2.1 Display

The LCD has a primary and secondary display.

• The primary display shows the measured pH, mV or Relative mV value.

• The secondary display shows the measured temperature.

The display also shows error messages, keypad functions and program functions.

See Figure 1.

Figure 1: Full LCD Screen

1. SETup mode indicator

2. MEASurement mode

indicator

3. CALibration indicator 9. Automatic Temperature

4. MEMory recall mode

indicator

5. mV or Relative mV indicator 11. ERRor indicator 17. HOLD indicator

6. pH indicator 12. MEMory location indicator 18. READY indicator

7. Temperature indicator (°F

available on pH 310 meter only)

8. pH buffer selection indicator 14. Probe indicator

Compensation indicator

10. Clock indicator (pH 310 meter only)

13. Low battery indicator

15. Buffer indicator

16. Function ON/OFF indicator

2.2 Keypad

The large membrane keypad makes the instrument easy to use. Each button, when pressed, has a

corresponding graphic indicator on the LCD. See Figure 2. Some buttons have several functions

depending on its mode of operation.

Key Function

ON/OFF

HOLD

MODE

CAL/MEAS

ENTER

MI/

▲ &

MR/

▼

SETUP

Powers on and shuts off the meter. When you switch on the meter, the meter starts up in the mode that you last switched off from. For example, if you shut the meter off in mV measurement mode, the meter will be in mV measurement mode when you switch the meter on.

Freezes the measured reading. To activate, press HOLD while in measurement mode. To release, press HOLD again.

pH 310 model only:

Selects the measurement parameter. Press MODE to toggle between pH, mV (or relative mV) and date/time [date/time feature available on pH 310 model only].

Toggles between Calibration and Measurement mode.

1. If you were in pH Measurement mode, press CAL/MEAS to enter pH Calibration mode.

NOTE: Temperature calibration is available from pH Calibration mode. See page 13 for directions.

2. If you were in mV Measurement mode, press CAL/MEAS to enter mV Calibration mode.

While in SETUP main menu, pressing CAL/MEAS takes you out directly into the measurement mode.

1. To confirm your calibration values in Calibration mode.

2. To confirm selections in SETUP mode.

In Measurement mode:

Press MI/ memory. Press MR/

In SETUP mode:

Press to scroll through the setup subgroup programs.

In mV calibration mode:

Press to adjust the calibration value.

Takes you into the SETUP mode. This mode lets you customize meter preference and defaults, and view calibration and electrode offset data.

When auto endpoint feature is switched on, it automatically holds reading

after 5 seconds of stability. The HOLD indicator appears on the display. Press HOLD to release auto endpoint feature.

▲ (memory input) to store values with its corresponding temperature values in the

▼ (memory recall) to retrieve data from memory.

Figure 2 - Keypad

2.3 Inse rting the Batteries

Four AAA batteries are included with your meter.

1. Use a Philips screwdriver to remove the two screws holding the battery cover. See Figure 3

below.

2. Remove battery cover to expose batteries.

3. Insert batteries. Follow the diagram inside the cover for correct polarity.

4. Replace the battery cover into its original position using the two screws removed earlier.

Figure 3 - Back panel of meter showing battery compartment

2.4 Connecting the Electrode and Temperature Probe

The waterproof pH 300/310 handheld pH meter uses any standard pH, ORP, or ISE electrode with a

BNC connector. For Automatic Temperature Compensation (ATC), this meter requires a

temperature probe with a special 6-pin connector.

Use either:

• All electrode with a BNC connector and a separate temperature probe with 6-pin connector

(EC-PHWPTEM-01W/ 35618-05).

• A “3-in-1” combination pH electrode with temperature probe designed specifically for pH 300

and pH 310 waterproof meters.

Refer to “Accessories” section on page 43 for more information on temperature probe and other

electrodes.

NOTE: Keep connectors dry and clean. Do not touch connector with soiled hands.

To connect the pH, ORP or ISE electrode:

1. Slide the BNC connector of the probe over the BNC connector socket on the meter. Make sure

the slots of the connector are in line with the posts of the socket. Rotate and push the

connector clockwise until it locks.

5. To remove electrode, push and rotate the connector counterclockwise. While holding onto the

metal part of the connector, pull it away from the meter.

CAUTION: Do not pull on the probe cord or the probe wires might disconnect.

To connect the temperature probe:

1. Line up the notch and 6 pins on the probe connector with the holes in the connector located on

the top of the meter. Push down and screw the metal sleeve to lock the probe connector into

place. See Figure 4.

6. To remove probe, unscrew the metal sleeve and slide up the probe connector. While holding

onto metal sleeve, pull probe away from the meter.

CAUTION: Do not pull on the probe cord or the probe wires might disconnect.

Figure 4 - Connection for pH Electrode (BNC) and Temperature probe (6-pin connector)

2.5 Attaching the Electrode Holder to the Meter

The waterproof pH 300/310 handheld meter comes complete with two

electrode holders. They are designed to facilitate one-hand operation.

Attach two electrode holders if you have a separate electrode and

temperature probe. Care must always be taken to avoid use of excessive

force in the process of attaching these components.

1. Locate the slot on the right-hand side of the meter.

2. Gently slide the flange of the

holder into the slot on the

meter. Make sure the holder is

secured properly into the slot.

See figure 5.

3. You can attach the electrode holder in different

positions. See figure 6.

To attach a second electrode holder:

4. Align the flange of the second electrode holder with

the slot of the first holder.

5. Slide the flange of the second holder into the slot of the

Figure 6 - Electrode can be placed in

different positions with holder

first holder until the tops of the holders are aligned and

secure. See figure 7.

Figure 5 - Holder

slot into right-hand

side of meter

2.6 Inserti ng the Electrode into the Electrode Holder

Do not use excessive force when inserting electrodes into the

holders.

Insert the pH electrode into the opening of the first holder until

the top housing of the electrode touches the top of the holder.

If you are using a separate temperature probe, insert the probe into the opening of the second holder

until the ridge on the housing touches the top of the holder.

NOTE: The holder is designed for probes 12 mm in diameter. Electrodes larger than

12 mm may not fit in the holder. Forcing the electrode into the opening may damage the holder or your electrode.

Figure 7 - Two holders

3 CALIBRATION

3.1 Important Information on Meter Calibration

When you re-calibrate your meter, old pH, Rel mV and mV calibration points are replaced on a point

by point basis. For example, if you previously calibrated your meter at pH 4.01, 7.00, and 10.01, and

you have now re-calibrated at pH 7.00, the meter retains the old calibration data at pH 4.01 and pH

10.01. To view current calibration points, see Program P2.0 in the SETUP section on page 26.

To completely re-calibrate your meter, or when you use a replacement probe, it is best to set the

meter to its factory defaults and re-calibrate the meter at all points. To reset the meter to its factory

defaults, see the SETUP section, Program P5.0, page 34 (for pH 300 meter) or Program P6.0, page

37 (for pH 310 meter).

3.2 Prep aring the Meter for Calib ration

Before starting calibration, make sure you are in the correct measurement mode. When you switch

on the meter, the meter starts up in the units last used. For example, if you shut the meter off in “mV”

units, the meter will read “mV” units when you switch the meter on.

Be sure to remove the protective electrode storage bottle or rubber cap of the electrode before calibration or measurement. If the electrode has been stored dry, wet the electrode in tap

water for 10 minutes before calibrating or taking readings to saturate the pH electrode surface and

minimize drift.

Wash your electrode in deionized water after use, and store in electrode storage solution. If storage

solution is not available, use pH 4.01 or 7.00 buffer solution.

Do not reuse buffer solutions after calibration. Contaminants in the solution can affect the calibration,

and eventually the accuracy of the measurements. See page 43 for information on our high-quality

pH buffer solutions.

pH 300 meter calibration

The pH 300 meter is capable of up to 5-point pH calibration to ensure accuracy across the entire pH

range of the meter. Select from the following buffer options:

• USA buffers --- pH 1.68; 4.01, 7.00, 10.01, and 12.45.

The meter automatically recognizes and calibrates to these standard buffer values, which makes pH

calibration faster and easier.

pH 310 meter calibration

The pH 310 meter features three separate internationally-recognized buffer standards. Select the

buffer standard you require in SETUP mode Program 4.0 (see pages 27 & 34 for more information).

The pH 310 meter is capable of up to 6-point pH calibration, depending on the buffer standard

selection. Select from the following buffer options:

• USA buffers --- pH 1.68, 4.01, 7.00, 10.01, and 12.45.

• NIST buffers --- pH 1.68, 4.01, 6.86, 9.18, and 12.45.

• DIN buffers --- pH 1.09, 3.06, 4.65, 6.79, 9.23, and 12.75.

The meter automatically recognizes and calibrates to these standard buffer values, which makes pH

calibration faster and easier.

3.3 pH Calibration

NOTE: We recommend that you perform at least

2-point calibration u sing stan dard bu ffers that bracket (one above and one below) the expected sample range. You can perform a 1-point calibration, but make sure that the buffer value is close to the sample value you are measuring.

4. If necessary, press the MODE key to select pH

mode. The pH indicator appears in the upper right

hand corner of the display.

5. Rinse the probe thoroughly with de-ionized water

or a rinse solution. Do not wipe the probe; this

causes a build-up of electrostatic charge on the

glass surface.

6. Dip the probe into the calibration buffer. The end

of the probe must be completely immersed into

the sample. Stir the probe gently to create a

homogeneous sample.

7. Press CAL/MEAS to enter pH calibration mode.

The CAL indicator will be shown. The primary

display will show the measured reading while the

smaller secondary display will indicate the pH

standard buffer solution.

8. Wait for the measured pH value to stabilize.

9. Press ENTER to confirm calibration. The meter is

now calibrated to the current buffer.

See figure 8.

Figure 8 - pH Calibration

7. Rinse the probe with de-ionized water or a rinse

solution, and place it in the next pH buffer.

8. Follow steps 5 and 6 for additional calibration

points. See Figure 9.

9. When all the calibration points as set in the Unit

Configuration Setup (see P4.2 on page 31) are

completed, the meter returns to Measurement

mode automatically. However, if you wish to

terminate the calibration without completing the

number of points as set in the Unit Configuration

Setup menu, press CAL/MEAS to return to pH

measurement mode.

NOTES:

To exit from pH calibration mode without confir ming calibration, DO NOT press ENTER in step 6. Press CAL/MEAS instead.

If the selected buffer value is not within ±1.0 pH from the

measured pH value: the electrode and buffer icon blink

and the ERR annunciator appears in the lower left corner

of the display. Figure 10.

To limit the number of pH buffer values available during

calibration, see SETUP section P4.2 on page 31.

Figure 9 - Next point calibration for pH

4.01

Figure 10 - Err message and electrode icon will appear if incorrect buffer are

used

3.4 Relati ve mV Calibration

10. While in the measurement function, press MODE to

enter the mV mode. The mV indicator appears in the

upper right hand corner. See figure at right.

11. Press the CAL/MEAS key. The CAL indicator

appears above the primary display. The primary

display shows the relative mV reading and the

secondary display shows the absolute mV value.

NOTE: If you have never calibrated relative mV or if

the meter has been reset, the value shown in the primary display is the same as the absolute mV value.

NOTE: “R.” annunciator will appear once mV

calibration is performed, an indication of mV offset.

12. Press the MI/▲ or MR/▼keys to enter the relative

mV value that matches your desired reading.

13. Press the ENTER key to confirm the reading and to

return to the measurement mode. The primary

display now shows the relative mV reading.

NOTES:

To view the mV offset value, use the SETUP mode

Program P3.1. See page 27 for instructions.

To reset all calibration and offset values in memory to the

factory default settings, use:

• In pH 300 meter: SETUP Program P 5.0. See page

34.

• In pH 310 meter: SETUP Program P 6.0. See page

37.

Figure 11 - mV calibration

3.5 Te mperature Calibration

The temperature sensor is factory calibrated. Calibrate the

temperature probe only if you suspect temperature errors that

may have occurred over a long period of time or if you have a

replacement probe.

Temperature Calibration

14. Make sure the ATC probe (or temperature connector of the

electrode) is attached to the 6-pin connector. The ATC

annunciator will appear at the right-hand side of the LCD.

15. Switch the meter on. Press the MODE key to select pH

mode.

16. Press the CAL/MEAS key to enter pH calibration mode.

The CAL indicator will appear above the primary display.

17. While in pH calibration mode, press the MODE key to enter

temperature calibration mode. The primary display shows

the temperature reading with last set offset and the

secondary display shows the factory default temperature

value.

18. Dip the ATC probe (or electrode) into a solution of known

temperature (i.e. a temperature bath). Allow time for the

temperature probe to stabilize.

19. Scroll with the MI/▲ or MR/▼ keys to set the correct

temperature value (i.e. the temperature of the temperature

bath). You can adjust the reading in increments of 0.1 °C.

20. Once you have selected the correct temperature, press the

ENTER key. The meter automatically returns to pH

measurement mode. See Figure 12.

NOTES:

• You can offset the temperature reading up to ±5°C from

original reading.

• To exit this program without confirming the temperature

calibration value, DO NOT press ENTER. Press

CAL/MEAS instead.

Figure 12 - Temperature

calibration

+ 36 hidden pages