Thermo Fisher Scientific PD 300 User Manual

Instruction Manual

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PD 300

Waterproof Hand-held pH/Dissolved Oxygen/Temperature Meter

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68X248910

Rev.3 JUN 06

Preface

This manual serves to explain the use of the PD 300 hand-held meter.

The manual functions as a step by step guide to help the user operate the meter and as a handy

reference guide.

This instruction manual is written to cover as many anticipated applications of the meter as

possible. If there are doubts in the use of the PD 300 meter, do not hesitate to contact the nearest

Authorised Distributor.

Eutech Instruments/ Oakton Instruments cannot accept any responsibility for damage or

malfunction to the meter caused by improper use of the instrument.

The information presented in this manual is subject to change without notice as improvements are

made, and does not represent a commitment on the part of Eutech Instruments Pte Ltd/ Oakton

Instruments.

Eutech Instruments Pte Ltd/ Oakton Instruments

Rev.3 JUN 06

TABLE OF CONTENTS

1 INTRODUCTION 1 2 DISPLAY AND KEYPAD FUNCTIONS 2

2.1 Display 2

2.2 Keypad 3

3 PREPARATION 4

3.1 Inserting the Batteries 4

3.2 Probe Information 5

4 CALIBRATION 7

4.1 Important Information on Meter Calibration 7

4.2 Temperature Calibration 8

4.3 pH calibration 9

4.4 Dissolved Oxygen Calibration 12

4.5 Dissolved Oxygen Calibration in % Saturation 13

4.6 Dissolved Oxygen Calibration in mg/L (ppm) mode 15

5 MEASUREMENT 17

5.1 Taking pH Measurements 17

5.2 Taking DO readings 21

5.3 Taking Pressure/Salinity Compensated DO measurements 23

6 HOLD FUNCTION 24 7 BACKLIT LCD 52

7.1 Using the backlit LCD 25

7.2 Adjusting the LCD brightness 25

8 ADVANCED SETUP FUNCTIONS 62

8.1 Advanced SETUP Mode Detailed Overview 26

8.2 P1.0: Viewing previous pH calibration data 30

8.3 P2.0: Viewing pH electrode data 31

8.4 P3.0: pH Measurement configuration 32

8.5 P4.0: Resetting to factory default settings (pH) 36

8.6 P1.0 (dPr): Dissolved Oxygen Parameters 37

8.7 P2.0 (OFS): Offset for % Saturation Measurement 40

8.8 P3.0 [P2.0] (CAL): Previous Calibration Information

(*Program numbers in “[ ]” are for setup in mg/L (ppm) mode. 41

8.9 P4.0 [P3.0] (ELE): Electrode Properties 42

8.10 P5.0 [P4.0] COF: Unit Configurati on 44

8.11 P6.0 [P5.0] LCd: Adjusting LCD Brightness 48

8.12 P7.0 [6.0] rSt: Resetting to Factory Default Setting (DO) 49

9 PROBE CARE AND MAINTENANCE 05

9.1 pH Electrode care 50

9.2 Dissolved Oxygen Principle 52

9.3 Probe Care and Maintenance 53

9.4 Membrane Housing Replacement 54

9.5 Membrane/O-ring Replacement (Optional Procedure) 55

9.6 Electrolyte Solution 57

9.7 DO Probe Troubleshooting Table 58

10 TROUBLE SHOOTING GUIDE 60 11 ERROR MESSAGES 61 12 SPECIFICATIONS 62 13 ACCESSORIES 63 14 ADDENDUM 1: DISSOLVED OXYGEN & METER THEORY 66 15 ADDENDUM 2: PRESSURE VS ALTITUDE TABLE 67

16 ADDENDUM 3: METER FACTORY DEFAULT SETTINGS 68 17 WARRANTY 69 18 RETURN OF ITEMS 70

Instruction Manual PD 300

1 INTRODUCTION

Thank you for selecting the PD 300 waterproof portable meter. This meter is a

microprocessor-based instrument that is designed to allow one-hand operation. It is capable of

measuring pH, Dissolved Oxygen (mg/L or % Saturation of Oxygen) and Temperature. It is

completely WATERPROOF --- and it FLOATS!

This meter has many user-friendly features ---- all of which are completely accessible through

the water-resistant membrane keypad. Your meter includes a pH electrode (EC-DA93506-

03B / 35805-23), dissolved oxygen electrode with integrated temperature sensor in a

submersible cable (EC-DOHANDY / 35640-50), maintenance kit, and instruction manual.

These items are available in a hard, plastic carrying case which makes it ideal for outdoor field

measurement. Please read this manual thoroughly before operating your meter.

Figure 1: Waterproof PD 300 Dissolved Oxygen Meter

Instruction Manual PD 300

2 DISPLAY AND KEYPAD FUNCTIONS

2.1 Display

The LCD has a primary and secondary display.

• The primary display shows the measured pH or DO value (either in mg/L, ppm or %, depending on units of measurement selected).

• The secondary display shows the measured temperature.

The display also shows error messages, keypad functions, and program functions. See Figure

Figure 2: Full LCD Screen

1. SETUP mode indicator 7. Oxygen solubility indicator in

2. MEASurement mode indicator 8. Temperature indicator 14.Calibration indicator

3. CALibration indicator 9. pH buffer indicator 15 Function ON / OFF

4. pH indicator 10. Automatic Temperature

5. % saturation indicator 11. ERRor indicator 17 READY indicator

6. ppt indicator for input of

Salinity value.

mg/L or ppm.

Compensation indicator

12. Low battery indicator

13. Probe indicator

indicator

16 HOLD indicator

Instruction Manual PD 300

2.2 Keypad

The large membrane keypad makes the instrument easy to use. Each button, when pressed,

has a corresponding graphic indicator on the LCD. See Figure 1. Some buttons have several

functions depending on its mode of operation.

Key Function

ON/OFF Powers meter on and off. The meter powers on in the mode that you were last using.

HOLD Freezes the measured reading. To activate, press HOLD while in measurement mode.

MODE 1. Selects the measurement parameter. Press MODE to toggle between pH; DO %

CAL/MEAS Toggles between Calibration and Measurement mode.

ENTER 1. Press to confirm your calibration values in calibration mode.

▲/▼

SETUP Takes you into the SETUP mode. This mode lets you customise meter preference and

For example, if you shut the meter off in mg/L measurement mode, the meter will be in mg/L measurement mode when switched on. Note: The meter shuts off automatically after 20 minutes of the last key press.

To release, press HOLD again. NOTE: When auto endpoint feature is switched on, it automatically holds reading after 5

seconds of stability. The HOLD indicator appears on the display. Press HOLD again to release.

saturation; and DO mg/L.

2. While in CAL mode under pH or mg/L (parts per million – ppm) measurement status, this key will toggle between pH or mg/L (or ppm) calibration and temperature calibration (refer to Section 4.2 in page 8).

1. In pH measurement mode, press CAL/MEAS to enter pH calibration mode.

2. In DO % saturation measurement mode, press CAL/MEAS to enter DO %

saturation calibration mode.

3. In DO mg/L (or ppm) measurement mode, press CAL/MEAS to enter DO mg/L (or

ppm) calibration mode.

4. To abort calibration, press CAL/MEAS key again to go back to measurement

mode.

While in advanced SETUP mode: Press CAL/MEAS to return to main menu from sub menus. Press CAL/MEAS again to return to return to measurement mode from main menu.

2. While in SETUP, pressing the ENTER key confirms selections.

In CALibration & SETUP mode:

In DO mg/L (or ppm) and temperature calibration mode, press up or down keys to increase or decrease the values.

In SETUP mode, press up or down keys to scroll through the setup subgroup programs. Within the SETUP sub-menu group, press up or down keys to change values.

defaults, view calibration and electrode data.

LIGHT. Press to activate backlit display.

Instruction Manual PD 300

3 PREPARATION

3.1 Inserting the Batteries

Four AAA batteries are included with your meter.

1. Use a Philips screwdriver to remove the two screws holding the battery cover. See Figure

3 below.

2. Remove battery cover to expose batteries.

3. Insert batteries. Follow the diagram inside the cover for correct polarity.

4. Replace the battery cover into its original position and secure into place using the two

screws removed earlier.

Figure 3: Back panel of meter showing meter compartment

Instruction Manual PD 300

3.2 Probe Information

Your meter includes two probes:

• pH electrode with BNC connector.

• DO/temperature probe with a notched 6-pin connector

The temperature sensing element built into the DO probe will also compensate for pH readings as long as both probes are in your solution at the same time.

You can use any standard pH electrode with a BNC connector with this meter. DO probes, “3-

in-1” pH electrodes with a built-in temperature element, and temperature probes require a

notched 6-pin connector (see Figure 4). For replacement probes, see the Accessories section.

If you want to use a “3-in-1” pH probe with a built-in temperature element, or if you want to use

a separate temperature probe, you will need to disconnect the DO probe to allow for

connection of the separate temperature sensor.

NOTE: Keep connectors dry and clean. Do not touch connector with soiled hands.

To connect the pH electrode:

1. Slide the BNC connector of the probe over the BNC connector socket on the meter.

Make sure the slots of the connector are in line with the posts of the socket. Rotate and

push the connector clockwise until it locks.

2. To remove electrode, push and rotate the connector counterclockwise. While holding

onto the metal part of the connector, pull it away from the meter.

See Figure 4. CAUTION: Do not pull on the probe cord or the probe wires may be damaged.

Instruction Manual PD 300

To connect the DO/ temperature probe:

1. Line up the notch and 6 pins on the probe connector with the holes in the connector

located on the top of the meter. Push down and turn the metal locking ring clockwise to

lock the probe connector into place. See Figure 4.

2. To remove probe, unscrew the metal locking ring by turning the ring counterclockwise

and slide up the probe connector. While holding onto metal sleeve, pull probe away from

the meter.

NOTE

Do not substitute other DO probes. For a replacement DO probe, see the “Accessories” section. Follow the same directions to connect an optional separate temperature element.

CAUTION: Do not pull on the probe cord or the probe wires may be damaged.

Figure 4: Connecting pH and DO/temperature probes

Instruction Manual PD 300

4 CALIBRATION

4.1 Important Information on Meter Calibration

When you recalibrate your meter, old calibration points are replaced on a “point by point” basis

in pH and completely for % saturation and mg/L (ppm).

For example:

• pH: if you previously calibrated your meter at pH 4.01, 7.00 and 10.01, and you

recalibrate at pH 7.00, the meter retains the old calibration data at pH 4.01 and pH 10.01.

• % saturation and mg/L (ppm): all new calibrations automatically override existing

calibration data.

To view current calibration points:

• pH: Program 1.0 in the SETUP section, page 30.

• % saturation: Program 3.0 in the SETUP section: page 41.

• mg/L (ppm): Program 2.0 in the SETUP section, page 41.

To completely recalibrate your meter, or when you use replacement probes, it is best to clear

old calibration data by resetting the meter.

To reset the meter to its factory defaults:

• pH: Program 4.0 in the SETUP section, page 36.

• % saturation: Program 7.0 in the SETUP section: page 49.

• mg/L (ppm): Program 6.0 in the SETUP section, page 49.

NOTE

Resetting the meter will set meter to factory defaults. pH, % saturation and mg/L (ppm) must be reset separately.

For directions on how to calibrate your meter:

See section 4.2 for Temperature Calibration

See sections 4.3 for pH calibration.

See section 4.5 for % saturation calibration

See sections 4.6 for mg/L (ppm) calibration

Instruction Manual PD 300

4.2 Temperature Calibration

The temperature sensor of the the DO probe is factory

calibrated. Calibrate your sensor only if you suspect

temperature errors that may have occurred over a long

period of time or if you have a replacement probe. Make sure

the DO probe is attached prior to performing the calibration.

1. Switch the meter on. Press MODE to select either pH or mg/L (ppm) measurement mode.

2. Press the CAL/MEAS key to enter pH or mg/L (ppm) calibration mode. The CAL indicator will appear above the primary display.

3. While in pH or mg/L (ppm) calibration mode, press the MODE key to enter temperature calibration mode. The primary display shows the temperature reading and the secondary display shows you the factory default temperature value.

4. Compare the primary display reading to a NISTtraceable thermometer or another thermometer known to be accurate.

5. Press the ▲ or ▼ keys to adjust the primary display reading to agree with your temperature standard.

6. Press the ENTER key to confirm temperature calibration and return to Measurement mode.

NOTE

• To exit from Temperature Calibration mode without

confirming calibration, DO NOT press ENTER in Step 6. Press CAL/MEAS instead.

• If a separate temperature probe is used for pH

measurement (EC-PHWPTEMP-01W / 35618-05), then temperature calibration done for pH is separate and independent from mg/L measurement mode.

• Te mperature calibration is restricted to ± 5°C from the

factory default value displayed during calibration (shown in the secondary display).

(The values shown above are for illustration purpose. Your meter may display different values depending on the settings & your environmental conditions)

Figure 5: Temperature calibration

Instruction Manual PD 300

4.3 pH calibration

Before starting calibration, make sure you are in pH measurement mode.

NOTE

We recommend calibration with at least two calibration standards that bracket (one above and one below) the expected sample range. One point calibration is possible as long as the chosen buffer is close to the expected sample value.

When you re-calibrate your meter, old calibration points are replaced on a “point-by-point”

basis. For example:

• if you previously calibrated your meter at pH 4.01, 7.00, and 10.01, and you re- calibrate at pH 7.00, the meter retains the old calibration data at pH 4.01 and pH

10.01.

Preparing for pH calibration

This meter is capable of up to 5-point pH calibration to ensure accuracy across the entire pH

range of the meter. Select from the following buffer options:

• pH 1.68, 4.01, 7.00, 10.01 and 12.45.

The meter automatically recognises and calibrates to these standard buffer values, which

makes pH calibration faster and easier.

Be sure to remove the protective electrode storage bottle or rubber ca p of the electrode before calibration or measurement. If the electrode has been stored dry, hydrate the

electrode in electrode storage solution or tap water for 10 minutes before calibrating or taking

readings to saturate the pH electrode surface and minimise drift.

Rinse your electrode in deionised water after use, and store in electrode storage solution. If

storage solution is not available, use fresh pH 4.01 or 7.00 buffer solution for short term

storage.

Do not reuse buffer solutions after calibration. Contaminants in the solution can affect the

calibration and eventually the accuracy of the measurements. See Accessories for information

on pH buffer solutions.

Instruction Manual PD 300

To Calibrate pH:

1. If necessary, press the MODE key to select

pH mode. The pH indicator appears in the

upper right hand corner of the display.

2. Rinse the probe thoroughly with de-ionised

water or a rinse solution. Do not wipe the

probe; this causes a build-up of electrostatic

charge on the glass surface.

3. Dip the probe into the calibration buffer. The

end of the probe must be completely

immersed into the sample. Stir the probe

gently to create a homogeneous sample.

NOTE

The temperature element is in the DO electrode. For temperature compensated readings, dip the DO electrode into the calibration buffer as well.

4. Press CAL/MEAS to enter pH calibration

mode. The CAL indicator will be shown. The

primary display will show the measured

reading while the smaller secondary display

will indicate the pH standard buffer solution.

5. Wait for the measured pH value to stabilise.

See Figure 5.

6. Press ENTER to confirm calibration. The

meter is now calibrated to the current buffer.

The lower display scrolls through the

remaining buffer options.

• For multi-point calibration, go to step 7.

• For one-point calibration, go to step 9.

Figure 6: pH Calibration

Instruction Manual PD 300

7. Rinse the electrode with deionised water or in

rinse solution, and place it in the next pH buffer.

8. Follow steps 5 to 8 for additional calibration

points. See Figure 7.

9. When calibration is complete, press

CAL/MEAS to return to pH measurement mode.

NOTE

To exit from pH calibration mode without confirming calibration, DO NOT press ENTER in step 6. Press CAL/MEAS instead.

If the selected buffer value is not within ±1.0 pH from the

measured pH value: the electrode and buffer icon blink

and the ERR annunciator appears in the lower left

Figure 7: Next point calibration for

pH 4.01

corner of the display. See Figure 8.

To limit the number of pH buffer values available during

calibration, see Setup Section 8.4

P3.2: Selecting

number of pH calibration points on page 34.

Figure 8: Err message and

electrode icon will appear if

incorrect buffer is used

Instruction Manual PD 300

4.4 Dissolved Oxygen Calibration

Before calibrating your meter, make sure to rinse the probe well with de-ionised water or rinse

solution.

NOTE

Do not let membrane surface of the probe touch any other surface. The probe guard (the piece with holes fitted over the end of the probe) protects the membrane; make sure this is always attached to the probe while it is in use. See Figure 9.

You can calibrate for dissolved oxygen in either % saturation or mg/L (ppm). All new calibrations

automatically override existing DO calibration data.

Figure 9: DO Probe guard

Before Calibration

Before starting calibration, make sure you are in the correct measurement mode. When you

switch on the meter, the meter starts up in the units last used (either pH, mg/L (ppm) or % air

saturation). For example, if you shut off the meter in “mg/L” units, the meter will read “mg/L”

units when you switch the meter on.

NOTE

Most users will calibrate to 100% saturation even when working in mg/L. When calibrating the meter in mg/L mode, you can make fine adjustments, typically to a mid-range value between 10 and 100%. If you are calibrating to a mid-range value, you need to set the 100% saturation value first.

Instruction Manual PD 300

4.5 Dissolved Oxygen Calibration in % Saturation

You can calibrate this meter quickly and easily in air. The exact calibration value depends on

barometric pressure. The meter is set to a factory default of 760 mm Hg, which results in a

calibration value of 100% saturation in air.

NOTE

If the barometric pressure setting has been changed from 760 mm Hg, the calibration value in air will automatically adjust to a value other than 100%. The adjusted value will be correct for the new barometric pressure setting.

See Section 8.6.1 on page 37 to change the pressure setting. See Addendum 2 on page 67

for a table of adjusted % saturation values.

To calibrate 100% Saturation:

1. Rinse the probe well with deionised rinse water. Do not touch

the membrane.

2. Press the MODE key to select the % saturation mode.

3. Press the CAL key. The CAL indicator will appear above the

primary display. The primary display shows the current value

of the measurement and the secondary display will show

100.0. See figure on right and NOTE above.

4. Hold the probe in the air. Wait for the reading to stabilise. If

the READY indicator feature is enabled, it will appear when

the reading is stable. (See page 45 for Ready indicator

setup.)

5. Press the ENTER key. The meter automatically calibrates to

100.0% air saturation and returns to Measurement mode.

NOTE

Whenever an error occurs during calibration, the ERR indicator appears in the lower left hand corner of the display.

You can offset your % DO calibration. See page 40 for directions.

Figure 10: To calibrate for

100% saturation

Instruction Manual PD 300

To calibrate 0% Saturation:

1. Rinse the probe well with deionised rinse water. Do not

touch the membrane.

2. Press the MODE key to select the % saturation mode.

3. Place the probe in zero oxygen solution. Stir the probe

gently to homogenise the sample. Make sure that the

READY

MEAS

128 . 5

23.4

°C ATC

sample is continuously flowing past the membrane sensor.

4. Press the CAL key. The CAL indicator will appear above

CAL

the primary display. The primary display shows the current

value of the measurement and the secondary display will

show 0.0.

5. Wait for the reading to stabilise. If the READY indicator

feature is enabled, it will appear when the reading is stable.

(See page 45 for Ready indicator setup.)

READY

MEAS

6. Press the ENTER key. The meter automatically calibrates

to 0.0% air saturation and returns to Measurement mode.

23.4

°C ATC

See figure on right.

NOTE

Figure 11: To calibrate for

0% saturation

The reading in the primary display in step 3 must read at 10% or under for the calibration to work correctly. Whenever an error occurs during calibration, the ERR indicator appears in the lower left hand corner of the display.

You can offset your % DO Calibration; see page 40 for directions.

Instruction Manual PD 300

4.6 Dissolved Oxygen Calibration in mg/L (ppm) mode

Calibrating the meter to 100% in saturation mode will also calibrate the mg/L mode at the

value in mg/L corresponding to 100% saturation. This should produce acceptable results in

most applications.

This meter also lets you make a calibration adjustment in mg/L (ppm) mode without affecting

your % saturation calibration. To select between mg/L and ppm units, see page 46.

NOTES ON MG/L (PPM) MODE CALIBRATION

• During mg/L (ppm) measurement and calibration, the meter adjusts to the

barometric pressure value that is programmed into the meter. It also adjusts to

the salinity value that is programmed into the meter.

• You can change the barometric pressure value and salinity value in the mg/L

(ppm) SETUP mode (see Section 8.6 -- P1.0 (dPr): Dissolved Oxygen

Parameters) for directions).

• Whenever an error occurs during calibration, the ERR indicator appears in the

lower left hand corner of the display.

Instruction Manual PD 300

To calibrate in mg/L (ppm) mode:

1. Calibrate 100% saturation as per Section 4.5.

2. Rinses the probe well with deionised rinse water. Wipe

the outside of the probe carefully (do not touch the

membrane).

3. Dip the probe into a sample of known oxygen

concentration. Wait for the reading to stabilise. If the

READY indicator feature is enabled, it will appear when

the reading is stable.

4. Press the MODE key to select the mg/L (ppm) mode.

5. Press the CAL key. The CAL indicator will appear above

the primary display. The primary display shows the

current value of the measurement and the secondary

display shows the temperature.

6. Press the ▲ and ▼ keys to adjust the reading.

7. Press the ENTER key. The meter automatically

calibrates to the value you entered and returns to

Measurement mode.

Figure 12: To calibrate in

mg/L (ppm) mode

(The values shown above

are for illustration purpose.

Your meter may display

different values depending

on the settings & your

environmental conditions)

Instruction Manual PD 300

5 MEASUREMENT

5.1 Taking pH Measurements

5.1.1

Automatic Temperature Compensation only occurs when a compatible temperature sensing

element is plugged into the meter. If there is no temperature sensor plugged into the meter, the default manual temperature

setting is 25 °C. You can manually set the temperature to match your working conditions

using a separate thermometer.

For automatic temperature compensation (ATC) simply

plug the temperature probe into the meter (see page 6

for directions). The ATC indicator will light up on the

LCD. See figure on right.

NOTE

The temperature sensing element must be in the sample that you are calibrating or measuring.

Automatic Temperature Compensation

Figure 13: Automatic Temperature

Compensation

5.1.2

Instruction Manual PD 300

Manual Temperature Compensation (pH only)

IMPORTANT: For manual compensation, you must

disconnect the temperature probe (see page 10).

1. Switch the meter on. Press the MODE key to

select pH mode.

2. Press the CAL/MEAS key to enter pH

calibration mode. The CAL indicator will

appear above the primary display.

3. While in pH calibration mode, press the MODE key to enter temperature calibration

mode. The primary display shows the

current temperature setting and the

secondary display shows the default value

25 °C.

4. Check the temperature of your sample using

an accurate thermometer.

5. Press the ▲ or ▼ keys to set the

temperature to the measured value from step

6. Press ENTER to confirm the selected

temperature and to return to the pH

measurement mode.

See Figure 14.

The meter will now compensate pH readings for the

manually set temperature.

Figure 14: Manual temperature

compensation

NOTE

To exit this program without confirming the manual temperature compensation value, DO NOT press ENTER in step 6. Press CAL/MEAS instead.

5.1.3

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