Thermo Fisher Scientific Panel 7-Plex User Manual
ProcartaPlex™ Human Antibody Isotyping
Panel 7-Plex
USER GUIDE
Catalog Number EPX070-10818-901
Publication Number MAN0024721
Revision A.0 (30)
For Research Use Only. Not for use in diagnostic procedures.
Bender MedSystems GmbH | Campus Vienna Biocenter 2 | 1030 Vienna, Austria
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The information in this guide is subject to change without notice.
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ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
End User License Agreement for Luminex Assay Products: By opening the packaging containing this Assay Product (which contains
fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are
consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at
https://www.luminexcorp.com/end-user-terms-and-conditions/#sec3. If you do not agree to all of the terms and conditions, you must
promptly return this Assay Product for a full refund prior to using it in any manner.
Revision history: Pub. No. MAN0024721
Revision Date Description
A.0 (30) 5 February 2021 new manual
TRADEMARKS: xMAP, Luminex 100/200, MAGPIX, INTELLIFLEX and FLEXMAP 3D are trademarks of Luminex Corp. and Bio-Plex is a
trademark of Bio-Rad Laboratories Inc. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise
specified.
©2021 Thermo Fisher Scientific Inc. All rights reserved.
Contents
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CHAPTER 1 ProcartaPlex™ Human Antibody Isotyping Panel 7-Plex .......... 4
Introduction .................................................................... 4
Contents and storage ............................................................ 5
Required materials not supplied ................................................... 5
Precautions and technical hints ................................................... 6
Workflow ....................................................................... 7
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CHAPTER 2 Methods ............................................................... 8
Sample preparation ............................................................. 8
Plasma sample preparation .................................................. 8
Serum sample preparation ................................................... 8
Cell culture supernatant preparation ........................................... 8
Dilution of serum, plasma, and cell culture supernatant samples .................. 9
Preparation of reagents .......................................................... 9
Prepare 1X Wash Buer ..................................................... 9
Prepare 1X Universal Assay Buer (UAB) ...................................... 9
Prepare 1X Detection Antibody Mix .......................................... 10
Prepare Standard Mix ...................................................... 10
Prepare 3-fold serial dilution ................................................ 10
Assay protocol ................................................................. 12
Instrument settings ............................................................. 14
Analyze results ................................................................ 14
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APPENDIX A Recommended plate layout ...................................... 16
ProcartaPlex
™
Human Antibody Isotyping Panel 7-Plex User Guide
3
1
WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear
appropriate protective eyewear, clothing, and gloves. Safety Data Sheets (SDSs) are available from
thermofisher.com/support.
Introduction
The ProcartaPlex™ Human Antibody Isotyping Panel 7-Plex has been optimized for detection of multiple
analytes from serum, plasma, and cell culture supernatants.
The panel is provided with individual vials of 1X capture and 50X detection reagents and is not
combinable with simplexes or other panels.
ProcartaPlex™ Human Antibody
Isotyping Panel 7-Plex
ProcartaPlex™ preconfigured panels are extensively tested for analyte combinability, interference and
cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex™ panels are
supplied with the necessary reagents to perform the assay.
Analytes
IgG1 IgG2 IgG3
IgG4 IgA IgE
IgM
For detailed product information, visit thermofisher.com/procartaplex
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ProcartaPlex™ Human Antibody Isotyping Panel 7-Plex User Guide
Contents and storage
Upon receipt, store the kit at 2°C to 8°C. When stored as indicated, all reagents are stable until the
expiration date.
Contents Amount
Isotyping Standard Mix (lyophilized) 2 each
Isotyping Detection Antibody Mix (50X) 1 x 70 μL
Capture Bead Mix (1X) 1 x 5 mL
Streptavidin-PE (SA-PE) (1X) 1 x 5 mL
Wash Buer (10X) 1 x 25 mL
Reading Buer (1X) 1 x 40 mL
Universal Assay Buer (10X) 1 x 35 mL
Detection Antibody Diluent (1X) 1 x 3 mL
Chapter 1
ProcartaPlex™ Human Antibody Isotyping Panel 7-Plex
Contents and storage
1
8-Tube Strip 2 each
Flat Bottom 96-well Plate, black 1 each
Microplate Lid 1 each
Plate Seals 8 each
Retain the lot-specific Certificate of Analysis that contains the product expiration date. The Certificate
of Analysis also contains important information such as bead number, analyte names and highest
standard concentration required for the assay setup on the xMAP instrument.
CAUTION! This kit contains materials with small quantities of sodium azide. Sodium azide reacts
with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a
large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin and
mucous membranes. In case of contact, rinse aected area with plenty of water. Observe all federal,
state and local regulations for disposal.
Required materials not supplied
• xMAP™ instrument
• Hand-Held Magnetic Plate Washer (Cat. No. EPX-55555-000)
• Deionized water
• Fresh cell culture medium for running cell culture supernatant samples
• Vortex mixer (e.g., Cat. No. 88882010)
• Microcentrifuge
ProcartaPlex™ Human Antibody Isotyping Panel 7-Plex User Guide
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Chapter 1 ProcartaPlex™ Human Antibody Isotyping Panel 7-Plex
1
Precautions and technical hints
• Adjustable single and multichannel pipettes with disposable tips and low volume reservoirs (e.g.,
Cat. No. 95128093)
• Beakers, flasks, and cylinders necessary for preparation of reagents
• Orbital microplate shaker with at least 1.5 mm or 0.059 inch orbit diameter capable of maintaining a
speed of 600 ± 50 rpm (e.g., Cat. No. 88882006)
Note: The use of rockers or large orbit shakers may cause adverse results.
Precautions and technical hints
1. Thoroughly read this User Guide and Certificate of Analysis prior to using the kit.
2. All chemicals should be considered potentially hazardous.
3. To avoid cross-contamination, do not invert the assay plate during the assay or allow contents
from one well to mix with another well.
4. Use a multichannel pipette and reagent reservoirs whenever possible to achieve optimal assay
precision.
5. Ensure that the xMAP™ instrument has been properly calibrated and set up prior to preparing and
running the assay.
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ProcartaPlex™ Human Antibody Isotyping Panel 7-Plex User Guide
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