Thermo Fisher Scientific Panel 11-Plex User Manual

ProcartaPlex™ Human Coronavirus Ig Total Panel 11-Plex

USER GUIDE

Catalog Number EPX110-16000-901

Publication Number MAN0024889

Revision A.0 (30)

For Research Use Only. Not for use in diagnostic procedures.

Bender MedSystems GmbH | Campus Vienna Biocenter 2 | 1030 Vienna, Austria For descriptions of symbols on product labels or product documents, go to thermoﬁsher.com/symbols-deﬁnition.

The information in this guide is subject to change without notice.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

End User License Agreement for Luminex Assay Products: By opening the packaging containing this Assay Product (which contains ﬂuorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at

https://www.luminexcorp.com/end-user-terms-and-conditions/#sec3. If you do not agree to all of the terms and conditions, you must

promptly return this Assay Product for a full refund prior to using it in any manner.

Revision history: Pub. No. MAN0024729

Revision Date Description

A.0 (30) 26 February 2021 new manual

TRADEMARKS: xMAP, Luminex 100/200, MAGPIX, INTELLIFLEX and FLEXMAP 3D are trademarks of Luminex Corp. and Bio-Plex is a trademark of Bio-Rad Laboratories Inc. All trademarks are the property of Thermo Fisher Scientiﬁc and its subsidiaries unless otherwise

speciﬁed.

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CHAPTER 1 ProcartaPlex™ Human Coronavirus Ig Total Panel 11-Plex ....... 4

Introduction .................................................................... 4

Contents and storage ............................................................ 5

Required materials not supplied ................................................... 5

Precautions and technical hints ................................................... 6

Workﬂow ....................................................................... 7

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CHAPTER 2 Methods ............................................................... 8

Sample preparation ............................................................. 8

Plasma sample preparation .................................................. 8

Serum sample preparation ................................................... 8

Dilution of plasma and serum samples ........................................ 8

Preparation of reagents .......................................................... 9

Prepare 1X Wash Buer ..................................................... 9

Prepare 1X Detection Antibody Mix ........................................... 9

Prepare Controls ............................................................ 9

Prepare High Control as standard curve (optional) ............................. 10

Assay protocol ................................................................. 11

Instrument settings ............................................................. 13

Analyze results ................................................................ 13

Data evaluation ................................................................ 14

Performance characteristics .................................................... 15

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APPENDIX A Recommended plate layout ...................................... 17

ProcartaPlex

Recommended plate layout ..................................................... 17

™

Human Coronavirus Ig Total Panel 11-Plex User Guide

WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear

appropriate protective eyewear, clothing, and gloves. Safety Data Sheets (SDSs) are available from

thermoﬁsher.com/support.

Introduction

The ProcartaPlex™ Coronavirus Ig Total Panel 11-Plex has been optimized for detection of multiple analytes from serum and plasma.

The panel is provided with individual vials of 1X capture and 50X detection reagents and is not combinable with simplexes or other panels.

ProcartaPlex™ Human Coronavirus

Ig Total Panel 11-Plex

ProcartaPlex™ preconﬁgured panels are extensively tested for analyte combinability, interference and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex™ panels are supplied with the necessary reagents to perform the assay.

Analytes

SARS-CoV2 Spike (trimer) Ig Total SARS-CoV2 S1 protein Ig Total SARS-CoV2 RBD Ig Total

SARS-CoV2 Nucleocapsid Ig Total SARS S1 Ig Total CoV-NL63 Ig Total

CoV-HKU1 Ig Total CoV-229E Ig Total MERS S1 Ig Total

CoV-OC43 Ig Total Negative Control

For detailed product information, visit thermoﬁsher.com/procartaplex

ProcartaPlex™ Human Coronavirus Ig Total Panel 11-Plex User Guide

Contents and storage

Upon receipt, store the kit at 2°C to 8°C. When stored as indicated, all reagents are stable until the expiration date.

Contents Amount

High Control 2 each

Medium Control 2 each

Low Control 2 each

Chapter 1

ProcartaPlex™ Human Coronavirus Ig Total Panel 11-Plex

Contents and storage

Detection Antibody Mix (50X)

Capture Bead Mix (1X) 1 x 5 mL

Wash Buer (10X) 1 x 25 mL

Reading Buer (1X) 1 x 40 mL

Assay Diluent (1X) 1 x 60 mL

Detection Antibody Diluent (1X) 1 x 3 mL

8-Tube Strip 2 each

Flat Bottom 96-well Plate, black 1 each

Microplate Lid 1 each

Plate Seals 8 each

Retain the lot-speciﬁc Certiﬁcate of Analysis that contains the product expiration date. The Certiﬁcate of Analysis also contains important information such as bead number, analyte names and highest standard concentration required for the assay setup on the xMAP instrument.

1 x 70 μL

CAUTION! This kit contains materials with small quantities of sodium azide. Sodium azide reacts

with lead and copper plumbing to form explosive metal azides. Upon disposal, ﬂush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin and mucous membranes. In case of contact, rinse aected area with plenty of water. Observe all federal, state and local regulations for disposal.

Required materials not supplied

• xMAP™ instrument

• Hand-Held Magnetic Plate Washer (Cat. No. EPX-55555-000)

• Deionized water

• Vortex mixer (e.g., Cat. No. 88882010)

• Microcentrifuge

ProcartaPlex™ Human Coronavirus Ig Total Panel 11-Plex User Guide

Chapter 1 ProcartaPlex™ Human Coronavirus Ig Total Panel 11-Plex

Precautions and technical hints

• Adjustable single and multichannel pipettes with disposable tips and low volume reservoirs (e.g., Cat. No. 95128093)

• Beakers, ﬂasks, and cylinders necessary for preparation of reagents

• Orbital microplate shaker with at least 1.5 mm or 0.059 inch orbit diameter capable of maintaining a speed of 600 ± 50 rpm (e.g., Cat. No. 88882006)

Note: The use of rockers or large orbit shakers may cause adverse results.

Precautions and technical hints

1. Thoroughly read this User Guide and Certiﬁcate of Analysis prior to using the kit.

2. All chemicals should be considered potentially hazardous.

3. To avoid cross-contamination, do not invert the assay plate during the assay or allow contents

from one well to mix with another well.

4. Use a multichannel pipette and reagent reservoirs whenever possible to achieve optimal assay

precision.

5. Ensure that the xMAP™ instrument has been properly calibrated and set up prior to preparing and

running the assay.

ProcartaPlex™ Human Coronavirus Ig Total Panel 11-Plex User Guide

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