Thermo Fisher Scientific MicroSEQ Reference Manual
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QUICK REFERENCE
MicroSEQ™ ID Software For SeqStudio™ Genetic Analyzer v1.0
Catalog Numbers A49380 and A49382
Pub. No. MAN0019555 Rev. A.0
This document contains basic information that is required to use the MicroSEQ™ ID Software For SeqStudio™ Genetic Analyzer. For more
information, see the MicroSEQ™ ID Software For SeqStudio™ Genetic Analyzer v1.0 User Guide (Pub. No. MAN0019554).
Product description
The Applied Biosystems™ MicroSEQ™ ID Software For SeqStudio™ Genetic Analyzer v1.0 is part of a streamlined workflow for microbial
identification of bacteria and fungi. MicroSEQ™ chemistry kits are used to generate PCR products that are sequenced on a SeqStudio™ Genetic
Analyzer. The MicroSEQ™ ID Software For SeqStudio™ Genetic Analyzer v1.0 compares the sequencing data to validated microbial libraries and
generates an identification report with percent similarity. The report includes a final identification list of the organisms that closely match the
unknown sample and can generate a phylogenetic tree. Security, audit, and e-signature capabilities are included to help enable 21 CFR Part 11
compliance.
Sign in to the MicroSEQ™ ID Software For SeqStudio™ Genetic Analyzer
To log in to the software:
1. Start the MicroSEQ™ ID Software For SeqStudio™ Genetic Analyzer by double-clicking the MicroSEQ ID icon
2. Enter your user name and password, then click OK.
Home screen
on the desktop.
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New Project
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Open Project
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SAE Manager
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For Research Use Only. Not for use in diagnostic procedures.
Create MicroSEQ ID Run
Open MicroSEQ ID Run
Open MicroSEQ ID Template
Workflow
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“Start the run on the SeqStudio™ Genetic Analyzer” on page 4
“Confirm that the MicroSEQ ID Run has started” on page 5
“Confirm that the run is complete” on page 6
“Analyze the data in the report” on page 7
“Selecting reports to export or print” on page 7
Create a MicroSEQ ID Run
1. On the home screen, click Create MicroSEQ ID Run.
The MicroSEQ ID Run window opens.
“Create a MicroSEQ ID Run” on page 2
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“Use SAE features” on page 7
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“Export a project” on page 7
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Project Setup
2. Enter the Project Setup details.
a. Enter the MicroSEQ ID Run Name.
b. Select the Run Module.
c. Select the Project Analysis Report.
d. Select the number of Matches To Display.
3. Click Add Project, then enter the project details.
a. Enter the New Project Name.
b. Enter the # of Specimens.
c. Select the Kit.
d. Select the Project Analysis Protocol.
e. Select the Library.
4. Click Next.
The Specimen Setup pane opens.
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Next
MicroSEQ™ ID Software For SeqStudio™ Genetic Analyzer v1.0 Quick Reference
5. (Optional) Edit the specimen names as required.
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Specimen Setup
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Specimen Name textbox
6. Click Next.
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Plate Layout
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Save button
The Plate Layout pane opens.
7. Click Save to save the run.
8. Click Export Plate.
A confirmation is received if the plate is exported successfully.
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Next
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Export Plate button
MicroSEQ
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ID Software For SeqStudio™ Genetic Analyzer v1.0 Quick Reference 3
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