Thermo Fisher Scientific KingFisher User Manual
KingFisher™ Apex Purification System
USER GUIDE
and BindIx Software version 1.0
Catalog Numbers 5400910, 5400920, 5400930, 5400940
Document Part Number
Publication Number MAN0018970
Revision A.0
For Laboratory Use.
Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Rev history: Pub. No. MAN0018970
Revision Date Description
A.0 28 January 2021 Baseline for KingFisher™ Apex Purification System User Guide revision
history.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2021 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■
CHAPTER 1 Product information .................................................. 6
Product description ............................................................. 6
Product type ................................................................... 6
Product components ............................................................ 7
Product overview ............................................................... 8
Instrument overview ......................................................... 8
User interface overview ..................................................... 10
Technology overview ........................................................... 11
Principle of magnetic particle processing ..................................... 11
Upon receiving the device ....................................................... 12
Guidelines for unpacking the instrument ..................................... 12
First time instrument setup .................................................. 13
System components ........................................................... 17
Heating block and corresponding plate ...................................... 17
Magnetic head ............................................................ 19
Tip comb and plate compatability ............................................ 21
About the Thermo Fisher™ Connect Platform ...................................... 21
Create a Connect account .................................................. 21
Connect the instrument to the Internet ....................................... 22
Create a PIN number ....................................................... 22
Generate a link code from the instrument ..................................... 22
Add an instrument to your Connect account .................................. 22
Set up a new Administrator ................................................. 24
■
KingFisher
CHAPTER 2 Methods ............................................................. 25
Protocol access overview ....................................................... 25
Download protocols through the instrument ................................... 25
Download protocols from Connect ........................................... 25
Run purification kit ............................................................. 26
Pause or abort run ............................................................. 27
Change heat block/magnetic head ............................................... 28
Unload heating block ....................................................... 28
Unload magnetic head ..................................................... 29
™
Apex Purification System User Guide
3
Contents
■
CHAPTER 3 Software applications .............................................. 30
About the user interface ........................................................ 30
Install BindIx™ Software .................................................... 30
Overview of UI controls ......................................................... 31
Home screen .............................................................. 31
Menu screen .............................................................. 32
Protocol editor screen ...................................................... 33
Settings screen ............................................................ 34
Instrument screen ......................................................... 39
Protocol overview .............................................................. 40
Create a protocol .............................................................. 40
Enter protocol information .................................................. 41
Select magnetic head ...................................................... 42
Add protocol steps ........................................................ 43
Define protocol steps ...................................................... 44
Save a protocol ................................................................ 56
Import/Export protocols ........................................................ 56
Import protocol from a USB memory device ................................... 56
Export protocol to a USB memory device ..................................... 56
Run history overview ........................................................... 56
Export run report to a USB memory device ................................... 57
Delete run report .......................................................... 57
Delete multiple run reports .................................................. 57
Delete run report by date ................................................... 57
Delete run report by name .................................................. 57
Telemetry data ................................................................. 58
Update software ............................................................... 59
■
APPENDIX A Maintenance ....................................................... 60
Regular and preventive maintenance ............................................. 60
Store the Transport Lock ........................................................ 60
Magnetic head maintenance ..................................................... 61
Decontamination procedure ..................................................... 61
Decontaminate instrument .................................................. 61
UV decontamination procedure .................................................. 62
UV treat process chamber .................................................. 62
Prepare instrument for transport ................................................. 63
Refit the transport lock ..................................................... 63
4
KingFisher™ Apex Purification System User Guide
■
APPENDIX B Specifications ...................................................... 64
Physical dimensions ............................................................ 64
Instrument specifications ....................................................... 64
Performance specifications ...................................................... 65
■
APPENDIX C Ordering information .............................................. 67
Accessory products ............................................................ 67
■
APPENDIX D Safety ............................................................... 69
Safety compliance ............................................................. 69
Symbols on this instrument ..................................................... 70
Standard safety symbols ................................................... 70
Electromagnetic compatibility (EMC) standards .................................... 71
Class B notice ............................................................. 71
EMC compliance .......................................................... 71
Electrical safety ................................................................ 72
Chemical safety ................................................................ 73
Biological hazard safety ......................................................... 74
Contents
■
APPENDIX E Certificate of decontamination ................................... 75
■
APPENDIX F Documentation and support ...................................... 76
Customer and technical support ................................................. 76
Limited product warranty ........................................................ 76
KingFisher™ Apex Purification System User Guide
5
1
Product description
The Thermo Scientific™ KingFisher™ Apex Purification System is intended for automated transfer and
processing of magnetic particles in a microplate format. The system utilizes magnetic rods covered with
a disposable, specially designed tip comb, and plates. The instrument functions without any dispensing
or aspiration parts or devices. Before the run, samples and reagents, including magnetic particles,
are dispensed into the plates according to default protocols that are downloaded from Thermo Fisher
Connect or http://www.thermofisher.com/kingfisher. Custom protocols can be created using the
instrument interface or with BindIx™ Software.
Product type
The KingFisher™ Apex Purification System consists of the KingFisher™ Apex instrument, and one of four
dierent magnetic heads and associated heating blocks.
Product information
Instrument
KingFisher™ Apex Purification System with 96 PCR Head 5400910
KingFisher™ Apex Purification System with 96 Combi Head 5400920
KingFisher™ Apex Purification System with 96 Deep Well Head 5400930
KingFisher™ Apex Purification System with 24 Combi Head 5400940
Catalog No.
6
KingFisher™ Apex Purification System User Guide
Product components
The contents of the KingFisher™ Apex Purification System are listed in the following table.
Chapter 1 Product information
Product components
1
Component
Instrument
KingFisher™ Apex Purification System 1 1 1 1
Accessory tray (See the KingFisher™ Apex Purification System Installation Guide)
KingFisher™ Apex Magnetic Head
96 PCR Head
96 Combi Head
96 Deep Well Head
24 Combi Head
KingFisher™ Apex PCR Heating Block 1 — — —
KingFisher™ Apex 96 Heating Block 1 1 1 —
KingFisher™ Apex 96 Deep Well Heating Block — 1 1 —
KingFisher™ Apex 96 Storage Tube Heating Block — 1 — —
KingFisher™ Apex 24 Deep Well Heating Block — — — 1
Cat. No.
5400910
1
—
—
—
Cat. No.
5400920
—
1
—
—
Cat. No.
5400930
—
—
1
—
Cat. No.
5400940
—
—
—
1
KingFisher™ Apex demo package (Contains plastic
consumables)
USB Drive containing KingFisher™ Apex user
manuals, BindIx™ Software, and Certificate of
conformance
UV Lamp 2 2 2 2
3 mm flat-tip screwdriver 1 1 1 1
Packing list 1 1 1 1
1 1 1 1
1 1 1 1
KingFisher™ Apex Purification System User Guide
7
1
3
4
6
5
2
Chapter 1 Product information
1
Product overview
Product overview
Instrument overview
The KingFisher™ Apex Purification System consists of an instrument with touch screen control and a
process chamber with lid to protect samples against environmental contamination.
Figure 1 KingFisher™ Apex instrument front view
1
Front lid
2
Touch screen
3
On/o button
4
USB port × 3 (Device)
Figure 2 KingFisher™ Apex instrument rear view
1
Power switch
2
Power inlet
3
Ethernet/LAN port
4
USB port (Host) × 2
5
RS-232 serial port
6
USB port (Device)
8
KingFisher™ Apex Purification System User Guide
Chapter 1 Product information
Product overview
1
Internal layout
The process chamber of the KingFisher™ Apex instrument consists of a turntable with eight plate
stations. The loading position has an illuminated display, while the processing position is situated over
the heating block (there are two heating block stations that can be selected for the processing position),
and underneath the processing head.
The processing head consists of two vertically moving platforms—the magnetic head holder, and the
tip comb holder. The magnetic head holder is used to attach up to two magnets composed of 24 or 96
magnetic rods, while the tip comb holder is used to hold plastic tip combs with 24 or 96 tips.
Figure 3 Process chamber layout
1
Turntable with plate stations (1–8)
2
Processing position
3
Loading position
4
Tip comb holder
5
Magnetic head holder
6
Shield plate
7
Barcode reader
8
UV light clip × 2
9
UV light socket × 2
KingFisher™ Apex Purification System User Guide
9
Chapter 1 Product information
1
Product overview
User interface overview
Symbol
Open Notifications screen (See page 31 for more details)
Swipe down from the top of the touch screen
Displays the following information:
•
Notifications
•
Internet status
•
Storage devices
Open Menu screen (See page 32 for more details)
Select Menu
Gives access to the following items:
•
Home
•
Protocol editor
•
Protocol library
•
Run history
Open Settings screen (See page 34 for more details)
Select Menu4Settings
Function
•
•
•
Import/Export
Settings
Instrument
Gives access to the following items:
•
Localization
•
Protocols
•
Sound
•
Loading position display
•
Connect
Open Instrument screen (See page 39 for more details)
Select Menu4Settings
Gives access to the following items:
•
Tools
•
Magnets
•
Heating blocks
•
Network share
•
About
•
Reset factory settings
•
Troubleshoot
•
View EULA
10
KingFisher™ Apex Purification System User Guide
Technology overview
Principle of magnetic particle processing
The KingFisher™ Apex instrument operates on inverse magnetic particle processing (MPP) technology.
Instead of transferring reagents in and out of plates as might occur with an external magnet method,
the magnetic particles are moved through a series of plates containing specific reagents with the aid of
magnetic rods covered with a disposable, specially designed plastic tip comb.
Chapter 1
Product information
Technology overview
1
Figure 4 Inverse magnetic particle processing
Working with magnetic particles can be divided into five separate processes:
• Collecting magnetic particles
• Releasing magnetic particles
• Washing magnetic particles
• Incubation
• Concentration
Collecting magnetic particles
During the collection of the magnetic particles, the magnetic rod is fully inside the tip. The magnetic
rods together with the tip comb move slowly up and down in the plate and the magnetic particles are
collected onto the edge of the tips. The magnetic rods together with the tip comb, having collected the
magnetic particles, can be lifted out of the plate and transferred into the next plate.
Releasing magnetic particles
After collection of the magnetic particles, the magnetic rods together with the tip comb are lifted from
the plate, the magnetic rods are lifted o and the tip comb is lowered into the next plate containing a
reagent.
Magnetic particles are released by moving the tip comb up and down several times at considerably high
speed until all the particles have been mixed with the substance in the next reaction.
KingFisher™ Apex Purification System User Guide
11
Chapter 1
1
Upon receiving the device
Product information
Washing magnetic particles
Washing the magnetic particles is a frequent and an important processing phase. Washing is a
combination of the release and collection processes in a plate filled with washing solution.
To maximize washing eciency, the magnetic rods together with the tip comb are designed to have
minimized liquid-carrying properties.
Incubation
To keep the magnetic particle suspension evenly mixed in long-running reactions, the tip comb can be
moved up and down in the solution.
Concentration
Magnetic particle processing allows changes in volume to be performed during the procedure.
Transferring the magnetic particles from a plate with a larger volume to a plate with a smaller volume
allows concentration of the sample to occur.
Upon receiving the device
• Check the enclosed packing list against order.
• Visually inspect the transport package, the instrument and the accessories for any damage
incurred during transit.
• If the carton has been damaged in transit, it is particularly important that you retain it for inspection
by the carrier in case there has also been damage to the instrument.
• Any damage claims must be filed with the carrier. Neither the manufacturer nor its agents can be
held responsible for any damage incurred in transit, but the manufacturer will make every eort to
help obtain restitution from the carrier. Upon receipt of the carrier's inspection report, arrangements
will be made for repair or replacement.
Guidelines for unpacking the instrument
• To prevent condensation, the instrument should be left in its protective, antistatic plastic wrapping
until the ambient temperature has been reached.
• The instrument weighs 56 kg (123 lbs) and requires at least two people to lift. Use the proper
precautions when lifting the instrument to avoid injury.
• Retain the original packaging and packing material for future transportation. The packaging is
designed to assure safe transport and minimize transit damage. Use of alternative packaging
materials may invalidate the warranty. Also retain all instrument-related documentation provided by
the manufacturer for future use.
• Refer to the installation guide for detailed instructions on how to unpack the instrument.
12
KingFisher™ Apex Purification System User Guide
First time instrument setup
3
2
1
2
1.
Connect the power supply cable to the power inlet.
The instrument operates at voltages of 100–240 VAC and the frequency range of 50/60 Hz. Ensure
that the local supply voltage in the laboratory conforms to that specified on the type label on the
back of the instrument
2.
Remove the transport lock screw from the magnet holder.
3.
Remove the transport lock screws from the transport lock plate over the tip comb holder.
Chapter 1 Product information
Upon receiving the device
1
4.
Remove the upper transport lock plate, then lift the tip comb holder and remove the lower
transport lock plate.
Do not discard the parts of the transport lock. The lock is required in case of future transportation
of the instrument (see “Store the Transport Lock” on page 60).
Install the UV light bulbs
1.
Insert the UV light bulb into the light clip.
KingFisher™ Apex Purification System User Guide
2.
Slide the light bulb back and into the light socket.
13
Chapter 1 Product information
1
Upon receiving the device
Turn on the instrument
1.
Turn on the power switch at the rear of the instrument.
2.
Press the on/o button to turn on the instrument.
14
KingFisher™ Apex Purification System User Guide
Chapter 1 Product information
Upon receiving the device
Install heating block
1.
Select Menu4Instrument.
2.
Select Insert for the position (1 or 2) to install the heating block.
3.
Scan the heating block to be installed with the barcode reader or perform manual selection with
the menu.
4.
Place the heating block on the heating platform. Fit the pins on the block into the slots in the
platform. Press the block until it clicks properly in position.
Note: Select Information for details on heating block installation.
1
5.
Select Next
KingFisher™ Apex Purification System User Guide
15
5
4
Chapter 1 Product information
1
Upon receiving the device
Install magnetic head
1.
Select Menu4Instrument.
2.
Select Insert for the position (1 or 2) to install the magnet.
3.
Scan the magnetic head to be installed with the barcode reader or perform manual selection with
the menu.
4.
Place the magnetic head onto the holder. Fit the pins on the head into the slots in the holder.
5.
Turn the handle to lock the magnet in place.
Note: Select Information for details on magnetic head installation.
6.
Select Next
16
KingFisher™ Apex Purification System User Guide
System components
Heating block and corresponding plate
The instrument uses specially designed barcoded plates for optimal processing. Use of other types of
plates may damage the instrument and compromise the warranty, as well as result in below optimal
performance.
The heating blocks are specifically designed for the plates listed in the following table to ensure even
heating during sample processing.
See “Change heat block/magnetic head” on page 28 for details on changing a heating block.
Heating block Plate
Chapter 1 Product information
System components
1
KingFisher™ Apex 24 Deep-Well Heating Block,
barcoded
KingFisher™ Apex 96 Deep-Well Heating Block,
barcoded
KingFisher™ 24 deep well plate, barcoded (30 µL to
KingFisher™ 96 Deep-Well Plate, barcoded (15–
1000 µL
5 mL
[1]
)
[1]
)
KingFisher™ Apex 96 Heating Block, barcoded KingFisher™ 96-Well Microplate, barcoded (15–
KingFisher™ Apex Purification System User Guide
200 µL
[1]
)
17
Chapter 1 Product information
1
System components
(continued)
Heating block Plate
KingFisher™ Apex PCR Heating Block, barcoded PCR plate (10–80 µL
AB2396
KingFisher™ Apex 96 Storage Tube Heating Block,
[2]
), or semi-skirted (Cat. No. AB2496 )
96 Storage Tube (Nunc™ 374086), 30–200 µL
barcoded
[1]
), skirted (Cat. No.
[2]
18
KingFisher™ Apex 24 Storage Tube Heating Block,
barcoded
24 Storage Tube (Nunc™ 364323), 200–1000 µL
KingFisher™ Apex Purification System User Guide
(continued)
KingFisher™ Apex 24 Storage Tube Adapter KingFisher™ Apex 96 PCR Semiskirted Plate Adapter
[1]
The fill volume depends on the type of tip comb being used.
[2]
Recommended PCR plate
Magnetic head
Chapter 1 Product information
Heating block Plate
Adapter
System components
1
WARNING! This product contains very strong permanent magnets. People wearing a pacemaker
or metallic prostheses should not use this product. A pacemaker or prostheses may be aected or
damaged if it comes in close contact with a strong magnetic field.
There are four kinds of interchangeable magnetic heads with corresponding disposable plastic tip
combs available for the instrument. The magnetic head holder has two positions that can be used to
hold dierent types of magnetic heads.
IMPORTANT! Keep magnetic heads away from each other and other magnets at all times. Clashing of
the magnets can cause serious damage to the magnets.
•
Do not place the magnetic head on top of the instrument or on any metal surfaces.
•
Do not use metal tools when handling magnetic heads.
•
Do not place the magnetic head in close proximity to magnetic tapes, computer discs or other
magnetic storage systems, such as credit cards, as damage can occur due to the strong magnetic
field of the magnetic heads.
•
Do not place the magnetic heads near a PC display, as this may cause damage to the display.
•
When not in use, keep the magnetic heads in their respective storage boxes.
•
See “Change heat block/magnetic head” on page 28 for details on changing a magnetic head.
KingFisher™ Apex Purification System User Guide
19
Chapter 1 Product information
1
System components
KingFisher™ Apex 96 PCR Head, barcoded KingFisher™ Apex 96 PCR Tip Comb, barcoded
Magnetic head Tip comb
KingFisher™ Apex 96 Deep-Well Head, barcoded KingFisher™ 96 Tip Comb for Deep-Well Magnets,
barcoded
KingFisher™ Apex 96 Combi Head, barcoded KingFisher™ Apex 96 Combi Tip Comb, barcoded
(also KingFisher™ 96 Tip Comb for Deep-Well
Magnets)
20
KingFisher™ Apex 24 Combi Head, barcoded KingFisher™ Apex 24 Combi Tip Comb, barcoded
(also KingFisher™ 24 Deep-Well Tip Comb,
barcoded)
KingFisher™ Apex Purification System User Guide
Tip comb and plate compatability
Chapter 1
About the Thermo Fisher™ Connect Platform
Product information
1
Tip comb
KingFisher™ 96 Tip Comb for Deep-Well Magnets KingFisher™ 96 Deep-Well plate
KingFisher™ Apex 96 Combi Tip Comb KingFisher™ 96 Deep-Well plate
KingFisher™ Apex 24 Deep-Well Tip Comb KingFisher™ 24 Deep-Well plate
KingFisher™ Apex 24 Combi Tip Comb 24 storage tube
KingFisher™ Apex 96 PCR Tip Comb PCR plate, skirted (Cat. No. AB2396
PCR plate, semi-skirted (Cat. No. AB2496
KingFisher™ 96-well Microplate (Cat. No. 97002540
Compatible plates
KingFisher™ 96-Well Microplate
KingFisher™ 96-Well Microplate
96 storage tube
KingFisher™ 24 Deep-Well plate
About the Thermo Fisher™ Connect Platform
The Thermo Fisher™ Connect Platform enables access to the KingFisher™ Apex instrument through
InstrumentConnect by way of a web browser or mobile device. This cloud-based tool allows the user to
perform the following functions when the instrument has Internet connectivity.
•
Monitor real-time instrument status.
•
Download protocols from the KingFisher™ Apex Protocol Library to run the instrument. Protocols in
the Protocol Library are optimized for kits from Thermo Fisher Scientific.
•
Upload your custom protocols to My Protocols in your Connect account.
•
Securely store, access and manage personal protocols and the Protocol Library.
•
Share protocols within a research team or with colleagues in another laboratory, location, or
country.
•
Automatically or manually upload run report from the instrument to your Connect account.
•
Manage multiple KingFisher™ Apex instruments remotely from a web-based dashboard.
•
Upgrade instrument software automatically, without hardware or manual updates.
Create a Connect account
1.
Go to thermofisher.com/connect from your web browser.
2.
Click Sign up now and follow the prompts to create an account.
Your e-mail address is used as your username.
KingFisher™ Apex Purification System User Guide
21
Chapter 1 Product information
1
About the Thermo Fisher™ Connect Platform
3.
When signed in, click Update PIN number.
4.
Enter a PIN number in the new and confirm fields.
The PIN number is necessary to sign in to Connect from the instrument.
Connect the instrument to the Internet
1.
Connect your instrument to the Internet.
•
Connect through the instrument Ethernet port using a cable.
•
Connect via wireless connection with a USB-enabled Wi-Fi dongle.
2.
Swipe down on the touchscreen to confirm that the instrument has an active network connection.
Create a PIN number
1.
Log in to your Connect account using a web browser.
2.
Navigate to
(InstrumentConnect).
3.
Select Update PIN number.
4.
Confirm the PIN number.
Generate a link code from the instrument
1.
Open the Notifications screen on the instrument.
2.
Select Connect to generate a link code and QR code.
3.
Copy down the link code generated by the instrument, or take a picture of the QR code with your
mobile device if you have a QR code scanner app installed.
Add an instrument to your Connect account
Connect supports access to the KingFisher™ Apex instrument with the InstrumentConnect application
on your mobile device or from a web browser. When the instrument is connected, real-time instrument
status can be viewed from the InstrumentConnect application.
IMPORTANT! The first Connect account that links to the instrument becomes Administrator by
default. If the first user needs to be unlinked from the instrument, a new user must be assigned
the Administrator role beforehand. Failure to do so will result in the loss of instrument connectivity
for all other linked users. For instructions on how to setup a new Administrator see “Set up a new
Administrator” on page 24.
22
KingFisher™ Apex Purification System User Guide
Chapter 1
About the Thermo Fisher™ Connect Platform
Product information
Add an instrument to your Connect account (PC)
1.
Log in to your Connect account using a web browser.
2.
Select (InstrumentConnect) from the left navigation strip.
3.
Select
4.
Select KingFisher™ Apex from the Instrument type drop down menu, then click Next.
5.
Enter the linking code generated by the instrument in the text box, then click Send.
Upon successful authentication, the instrument is linked to Connect.
(Add an Instrument) from the top navigation strip.
Add an instrument to your Connect account with linking code (mobile device)
1.
Open the InstrumentConnect application on a mobile device.
2.
Select +.
1
3.
Select Linking code.
4.
Enter the linking code obtained from the instrument.
5.
Select Send.
Add an instrument to your Connect account with QR code (mobile device)
Install a QR code scanner app on your mobile device to connect to the instrument using the QR code.
1.
Open the InstrumentConnect application on a mobile device.
2.
Select QR code.
3.
Take a picture of the QR code on the Notifications screen of the instrument with your mobile
device.
Access your Connect account from an instrument
1.
Swipe down to open the Notifications screen.
2.
Select Sign in.
Note: If another user account is displayed, select the username to sign out and connect a
dierent user account.
3.
Select your username from the list of linked accounts.
4.
Enter your Connect PIN number.
If you do not have a PIN number, set the PIN number in the dialog box.
5.
Select OK.
KingFisher™ Apex Purification System User Guide
23
Chapter 1 Product information
1
About the Thermo Fisher™ Connect Platform
Set up a new Administrator
1.
Log the current Administrator into their Connect account.
2.
Select Instruments
3.
Select the KingFisher™ Apex instrument that the user is linked to.
4.
Select Manage users.
5.
Set Administrator privileges to another user linked to the same instrument.
24
KingFisher™ Apex Purification System User Guide
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