Thermo Fisher Scientific DO 300 User Manual

gyM

sy...

Instruction Manual

DO 300

Waterproof Hand-held Dissolved Oxygen / Temperature Meter

Technolo

adeEa

68X248909

Rev 3 02/04

Preface

This manual serves to explain the use of the Waterproof DO 300 hand-held meter. It functions in two ways, firstly as a step by step guide to help you to operate th e meter. Secondly, it serves as a handy reference guide. It is written to cover as many anticipated applications of the Waterproof DO 300 meter as possible. If there are doubts in the use of the meter, please do not hesitate to contact the nearest Authorised Distributor.

Eutech Instruments/ Oakton Instruments cannot accept any responsibility for damage or malfunction to the meter caused by improper use of the instrument.

The information presented in this manual is subjected to change without notice as improvements are made, and does not represent a commitment on the part of Eutech Instruments Pte Ltd/ Oakto n Instruments.

Eutech Instruments Pte Ltd/ Oakton Instruments

Rev 3 02/04

TABLE OF CONTENTS

1 INTRODUCTION 1 2 DISPLAY AND KEYPAD FUNCTIONS 2

2.1 Display 2

2.2 Keypad 3

3 PREPARATION 4

3.1 Inserting the Batteries 4

3.2 Connecting the Probe 5

4 CALIBRATION 6

4.1 Preparing the meter for calibration 6

4.2 Temperature Calibration 7

4.3 Dissolved Oxygen Calibration in % Saturation 8

4.4 Dissolved Oxygen Calibration in mg/L (ppm) mode 10

5 MEASUREMENT 11

5.1 Taking DO readings 11

5.2 Taking Pressure/Salinity Compensated DO measurements 13

6 HOLD FUNCTION 14 7 MEMORY FUNCTIONS 15

7.1 Memory Input 15

7.2 Memory Rec all 16

8 ADVANCED SETUP FUNCTIONS 17

8.1 Advanced SETUP Mode Detailed Overview 18

8.2 Clr: Memory Clear 20

8.3 dPr: Dissolved Oxygen Parameters 21

8.4 OFS: Offset for % Saturation Measurement 24

8.5 CAL: Previous Calibration Information 25

8.6 ELE: Electrode Properties 26

8.7 COF: Unit Configuration 28

8.8 LCd: Adjusting LCD Brightness 32

8.9 CLO: Setting the Real-Time Clock 33

8.10 rSt: Resetting to Factory Default Setting 35

9 PROBE CARE AND MAINTENANCE 36

9.1 Probe Care 37

9.2 Membrane Housing Replacement 38

9.3 Membrane/O-ring Replacement (Optional Procedure) 39

9.4 Electrolyte Solution 41

9.5 DO Probe Troubleshooting Table 42

10 TROUBLE SHOOTING GUIDE 44 11 ERROR MESSAGES 45 12 SPECIFICATIONS 46 13 ACCESSORIES 47 14 DISSOLVED OXYGEN & METER THEORY 49 15 PRESSURE VS ALTITUDE TABLE 50 16 FACTORY DEFAULT SETTINGS 51 17 OVER-VIEW OF SETUP FUNCTIONS 52 18 WARRANTY 56 19 RETURN OF ITEMS 56

Instruction Manual DO 300

1 INTRODUCTION

Thank you for selecting the DO 300 waterproof portable meter. This meter is a microprocessor-based instrument that is designed to be handy and capable of allowing onehand operation. It is capable of measuring Dissolved Oxygen in mg/L or ppm, % Saturation of Oxygen and Temperature. It is completely WATERPROOF --- and it FLOATS!

This meter has many user-friendly features ---- all of which are completely accessible through the water-resistant membrane keypad. Your meter includes a dissolved oxygen / temperature probe with submersible cable (inclusive maintenance kit), instruction manual, and a warranty card. Please read this manual thoroughly before operating your meter.

Figure 1: Waterproof DO 300 Dissolved Oxygen Meter

Instruction Manual DO 300

2 DISPLAY AND KEYPAD FUNCTIONS

2.1 Display

The LCD has a primary and secondary display.

o The primary display shows the measured DO value either in mg/L, ppm or %,

depending on units of measurement selected.

o The secondary display shows the measured temperature in °C.

The display also shows error messages, keypad functions and program functions. See Figure

Figure 2: Full LCD Screen

1. SETUP mode indicator 8. Temperature indicator 14. Probe indicator

2. MEASurement mode indicator 9. Automatic Temperature

3. CALibration indicator 10. Clock indicator 16. DO probe cell factor

4. MEMory recall mode indicator 11. ERRor indicator 17. Function ON / OFF

5. % saturation indicator 12. MEMory location indicator 18. HOLD indicator

6. ppt indicator for input of

Salinity value.

7. Oxygen solubility indicator in mg/L or ppm.

Compensation indicator

13. Low battery indicator 19. READY indicator

15. Calibration indicator

indicator

Instruction Manual DO 300

2.2 Keypad

The large membrane keypad makes the instrument easy to use. Each button, when pressed, has a corresponding graphic indicator on the LCD. See Figure 1. Some buttons have several functions depending on its mode of operation.

Key Function

ON/OFF Powers meter on and off. The meter powers on in the mode that you were last using.

HOLD Freezes the measur ed reading. To activate, press HOLD while in measurement mode.

MODE 1. Selects the measurement parameter. Press MODE to toggle between DO %

CAL/MEAS Toggles between Calibration and Measurement mode.

ENTER 1. Press to confirm your calibration values in calibration mode.

MI/ & MR/

SETUP Takes you into the SETUP mode. This mode lets you customise meter preference and

For example, if you shut the meter off in mg/L measurement mode, the meter will be in mg/L measurement mode when switched on. Note: The meter shuts off automatically after 20 minutes of the last key press.

To release, press HOLD again.

NOTE

: When auto endpoint feature is switched on, it automatically holds reading after 5 seconds of stability. The HOLD indicator appears on the display. Press HOLD to release auto endpoint feature.

saturation; DO mg/L (or ppm) and date/time.

2. While in CAL mode under mg/L (or ppm) measurement status, this key will toggle

between mg/L (or ppm) calibration and temperature calibration (refer to section 4.2)

1. In DO % saturation measurement mode, press CAL/MEAS to enter DO %

saturation calibration mode.

2. In DO mg/L (or ppm) measurement mode, press CAL/MEAS to enter DO mg/L (or

ppm) calibration mode.

3. To abort calibration, press CAL/MEAS key again to go back to measurement

mode.

While in advanced SETUP mode: Press CAL/MEAS to return to main group menu from sub group menus. Press CAL/MEAS again to return to measurement mode from main menu.

2. While in SETUP mode, pressing the ENTER key confirms the main group menu

selection and takes you through each sub group menu. In Measurement mode: Press MI key to store the mg/L (ppm) or % Saturation values with its corresponding

temperature and date/time values, into the memory. Press MR to retrieve data from memory in LIFO method.

In Calibration & Set up mode:

In DO mg/L (or ppm) and temperature calibration mode, press  or  keys to increase or decrease the calibration values.

In SETUP mode, press  or  keys to scroll through the SETUP main group menu and to set the parameters in the sub group menu.

defaults, view calibration and electrode data. LIGHT : Press to light up the backlit display.

Instruction Manual DO 300

3 PREPARATION

3.1 Inserting the Batteries

Four AAA batteries are included with your meter.

1. Use a Philips screwdriver to remove the two screws holding the battery cover. See Figure 3 below.

2. Remove battery cover to expose batteries.

3. Insert batteries. Follow the diagram inside the cover for correct polarity.

4. Replace the battery cover into its original position and secure into place using the two screws removed earlier.

Figure 3: Back panel of meter showing meter compartment

Instruction Manual DO 300

3.2 Connecting the Probe

Your meter uses a dissolved oxygen/temperature probe. The probe cable has a notched 6-pin connector to attach the probe to the meter.

NOTE: Do not substitute other probes. For a replacement probe, see the “Accessories” section, page 47- 48.

NOTE: Keep connector dry and clean. Do not touch connector with soiled hands. To connect the probe:

1. Line up the notch and 6 pins on the meter’s connector with the holes in the probe’s connector. Push down and turn the locking ring to lock into place. See Figure 4 below.

2. To remove probe, unscrew the metal locking ring by turning the ring counterclockwise and slide up the probe connector. While holding onto metal sleeve, pull probe away from the meter.

CAUTION: Do not pull on the probe cord or the probe wires might disconnect.

Figure 4: Connect DO Probe

Instruction Manual DO 300

4 CALIBRATION

4.1 Preparing the meter for calibration

The amount of oxygen dissolved in water will depend on its temperature, atmospheric pressure and its salinity. While the pressure and salinity values are manually entered into the instrument, the temperature is being measured by the probe. It is therefore very important that the temperature is calibrated if necessary prior to the DO calibration.

The measurements of % Saturation of DO will linearly affect the measurement for DO in mg/L. Hence calibration in % Saturation of DO should be carried out first.

Before calibrating your meter, make sure to rinse the probe well with DI (de-ionised) water or rinse solution.

NOTE: Do not let membrane surface of the probe touch any other surface. The probe guard (the piece with holes fitted over the end of the probe) protects the membrane; make sure this is always attached to the probe while it is in use.

You can calibrate for dissolved oxygen in either % saturation or mg/L (ppm). When you recalibrate your meter in % saturation mode, old calibrations in % saturation will be replaced while the % saturation offset adjustment will be erased. However, recalibration in the mg/L (ppm) concentration mode will only replace the old calibration in the mg/L (ppm) mode and do not affect the calibration in the % saturation mode.

Figure 5: DO Probe guard

Before Calibration

Before starting calibration, make sure you are in the correct measurement mode. When you switch on the meter, the meter starts up in the mode last used (either in mg/L (ppm) or % Saturation measurement mode or clock). For example, if you switch the meter off while it is in the “mg/L” measurement mode, it will also be in the “mg/L” measurement mode when you switch the meter on.

Instruction Manual DO 300

4.2 Temperature Calibration

DO in mg/L is dependent on temperature, so it is first necessary to verify the temperature reading. Temperature calibration is accessible from the mg/L or ppm measurement mode.

The built-in temperature sensor in the probe is factory calibrated. Calibrate your sensor only if you suspect temperature errors may have occurred over a long period of time or if you have a replacement probe.

1. Dip the DO probe into a solution with known temperature.

Allow sufficient time for the temperature to stabilise.

NOTE: Use an accurate temperature meter or bath to determine the solution’s temperature.

2. Ensure that the meter is in measurement mode. Press MODE

key if necessary to select mg/L mode.

3. Press CAL key to go into calibration mode of mg/L. The

primary display shows the measured DO in mg/L and the secondary display shows the temperature.

4. Press MODE key to go into temperature offset adjustment

mode. The primary and secondary displays will show the temperature values.

5. Press MI/ or MR/ key to set the correct temperature value.

NOTE: Temperature calibration is restricted to ± 5°C from the factory default value displayed during calibration (shown in the secondary display).

6. Press ENTER key to confirm the adjusted temperature.

NOTE: To exit from Temperature Calibration mode without confirming calibration, DO NOT press ENTER in Step 6. Press CAL/MEAS instead.

Since temperature readings affect the accuracy of the mg/L measurements, it is strongly recommended to carry out a mg/L calibration after a temperature calibration is done.

(The values shown above are for illustration purpose. Your meter may display different values depending on the settings & your environmental conditions)

Instruction Manual DO 300

4.3 Dissolved Oxygen Calibration in % Saturation

You can calibrate this meter quickly and easily in air. The exact calibration value depends on barometric pressure. The meter is set to a factory default of 760 mm Hg, which results in a calibration value of 100% saturation in air.

NOTE: If the barometric pressure setting has been changed from 760 mm Hg, the calibration value in air will automatically adjust to a value other than 100%. The adjusted value will be correct for the new barometric pressure setting.

See page 21 to change the pressure setting. See Pressure vs Altitude Table on page 50 for a table of adjusted % saturation values.

To calibrate 100% Saturation:

1. Press MODE key to select % Saturation mode.

2. Rinse the probe well with deionised rinse water. For best

accuracy, wipe the end of the probe dry. Do not touch the membrane.

3. Hold the probe in the air with the sensor facing down an d

press CAL key to calibrate the meter. The LCD will show a “CAL” mode. The primary display will show the current value of measurement and the secondary display will show “100.0” to which the meter is going to be calibrated. Wait for the reading to stabilise.

4. Press ENTER key to confirm the calibration. The meter

automatically calibrates to 100% air saturation and returns to the measurement mode.

NOTES:

Whenever an error occurs during calibration, the ERR indicator appears in the lower left hand corner of the display.

You can offset your % DO calibration. See section 8.4 - OFS: Offset for % Saturation Measurement on page 24 for directions.

Figure 6: To calibrate for

100% saturation

Instruction Manual DO 300

To calibrate 0% Saturation:

1. Rinse the probe well with DI rinse water. For best accuracy, wipe the end of the probe dry. Do not touch the membrane.

2. Press the MODE key to select the % saturation mode.

3. Place the probe in zero oxygen solution. Stir the solution gently using the probe to homogenize the sample. Make sure that the sample is continuously flowing past the membrane sensor.

READY

MEAS

8.5

23.4

CAL

°C ATC

4. Press the CAL key. The CAL indicator will appear above the primary display. The primary display will show the factory default % saturation measurement and the secondary display will show 0.0.

READY

MEAS

5. Wait for the reading to stabilise. If the READY indicator feature is enabled, it will appear when the reading is stable. (See section 8.7.1 on page 29 for Ready indicator setup).

6. Press the ENTER key. The meter automatically calibrates to 0.0% saturation and returns to Measurement mode.

See figure on right.

NOTE

23.4

Figure 7: To calibrate for

0.0% saturation

°C ATC

The factory default % saturation measurement in step 4 must read at 10% or under for the calibration to work correctly. Whenever an error occurs during calibration, the ERR indicator appears in the lower left hand corner of the display.

Instruction Manual DO 300

4.4 Dissolved Oxygen Calibration in mg/L (ppm) mode

Calibrating the meter to 100% in saturation mode will also calibrate the mg/L mode at the value in mg/L corresponding to 100% saturation. This should produce acceptable results in most applications.

This meter also lets you make a calibration adjustment in mg/L (ppm) mode without affecting your % saturation calibration.

To select between mg/L and ppm units, see page 30.

To calibrate in mg/L (ppm) mode:

1. Calibrate 100% saturation as per Section 4.3.

2. Rinse the probe well with DI rinse water or rinse solution.

For best accuracy, wipe the end of the probe dry. Do not touch the membrane.

3. Press the MODE key to select the mg/L (ppm) mode.

4. Dip the probe into a sample of known oxygen concentration.

Wait for the reading to stabilise. If the READY indicator feature is enabled, it will appear when the reading is stable.

5. Press the CAL key. The CAL indicator will appear above the

primary display. The primary display will show the factory default mg/L (ppm) measurement and the secondary display shows the temperature.

6. Press the MI/ or MR/ keys to set the reading according

to the sample value.

7. Press the ENTER key. The meter automatically calibrates to

the value you entered and returns to Measurement mode.

Notes on mg/L (ppm) mode calibration

• During mg/L (ppm) measurement and calibration, the meter adjusts to the barometric pressure value and the salinity value that is programmed into the meter

• You can change the barometric pressure value and salinity value in the mg/L (ppm) SETUP mode (see section 8.3 on page 21 for directions).

• Whenever an error occurs during calibration, the ERR indicator appears in the lower left hand corner of the display.

Figure 8: To calibrate in

mg/L (ppm) mode

(The values shown above

are for illustration purpose.

Your meter may display

different values depending

on the settings & your

environmental conditions)

Instruction Manual DO 300

5 MEASUREMENT

5.1 Taking DO read in gs

During measurement, the probe can be:

z Fully immersed in the solution z Partially immersed in the solution

Do not allow the probe’s membrane surface to touch anything! It is always advisable to insert the probe guard (refer to Figure 34 and 35 on pages 38 and 39)

IMPORTANT: Since the DO probe consumes oxygen from the sample, the sample must constantly flow past the membrane to achieve more accurate readings. You can use a stirrer to accomplish this.

To take measurements:

1. Rinse the probe well with DI rinse water or rinse solution.

2. Select the appropriate measurement mode. Press the MODE key to toggle between modes:

z % Saturation z mg/L (or ppm) z date/time clock

3. Dip the probe into the sample. Stir the solution gently using the probe to homogenise the sample. Make sure that the sample is continuously flowing past the membrane sensor.

4. Note the reading on the display. If the READY indicator is switched on, it will appear when the reading is stable.

5. You can press the

(LIGHT) key to activate the backlit

LCD. This helps you read measurements in dimly lit areas.

Figure 9: Change

measurement mode

Figure 10: Taking

measurement

Instruction Manual DO 300

NOTE: To select between mg/L and ppm units, see Section 8.7.2 on page 30. The ATC indicator should appear in the lower right hand corner of the display. If it does not,

this indicates an error.

Taking measurements with READY ON indicator selected on

If the READY indicator has been activated, the READY annunciator lights when the reading is stable.

To enable or disable the READY indicator – see Section 8.7.1 on page 29 for directions.

Taking measurements with READY HOLD indicator selected on

When a reading is stable for more than 5 seconds, the auto endpoint feature will automatically “HOLD” the reading. The “HOLD” indicator appears on the left side of the display. Press the HOLD key to release the reading.

To enable or disable the Auto endpoint feature– see Section 8.7.1 on page 29 for directions.

(The values shown above are for illustration purpose. Your meter may display different values depending on the settings & your environmental conditions)

Figure 11 – Auto endpoint

feature

Instruction Manual DO 300

5.2 Taking Pressure/Salinity Compensated DO measurements

If necessary, you can adjust the pressure and salinity values of your measurements in the SETUP mode. The DO meter will automatically compensate for salinity and pressure based on the values entered in the SETUP functions. The meter is factory set at 760 mm Hg (101.3 kilo Pascals) pressure adjustment and a factor of 0.0 ppt salinity adjustment. See Section 15 on page 50 for a “Pressure vs Altitude” table.

Pressure Adjustment

1. Press the SETUP key to enter SETUP mode.

2. Press the MI/ key once. The upper display shows “dPr”.

3. Press the ENTER key until the upper display shows a number and the lower display shows “Hg” or “PA”.

4. Use the MI/ and MR/ keys to enter the barometric pressure. The upper display will show the value you have entered.

5. Press ENTER to confirm pressure value.

6. Press CAL/MEAS to return to Measurement mode, or continue with step 5 on page 23 to make a salinity adjustment [available from mg/L (ppm) SETUP mode only].

7. See Figure 18 on page 22.

Salinity Adjustment NOTE: This mode appears in mg/L (or ppm) SETUP mode only.

1. Press the MODE key to select mg/L (ppm) mode.

2. Press the SETUP key to enter SETUP mode.

3. Press the MI/ key once. The upper display shows “dPr”.

4. Press the ENTER key until the upper display shows a number and the lower display shows “SAL”.

5. Use the MI/ or MR/ keys to enter the salinity of your solution in ppt. The upper display will show the value you have entered. Salinity correction values from 0.0 to 50.0 ppt can be entered.

6. Press ENTER key to confirm value.

7. Press CAL/MEAS to return to Measurement mode.

8. See Figure 19 on page 23.

Instruction Manual DO 300

6 HOLD FUNCTION

This feature lets you freeze the dissolved oxygen and temperature readings for a delayed observation. HOLD can be used any time when in MEAS mode.

To hold a measurement, press the HOLD key while in measurement mode. “HOLD” will appear on the display.

To release the held value, press HOLD again. Continue to take measurements.

NOTE: This meter shuts off automatically 20 minutes after the last key pressed. If the meter is shut off either automatically or manually, the HOLD value will be lost. For longer storage, use the memory functions.

NOTE: This meter has an auto endpoint feature. When this feature is switched on, the display will automatically “HOLD” a reading that has been stable for more than 5 seconds. The “HOLD” indicator appears. Press the HOLD key to release the reading. To switch on or off the auto endpoint feature, see Section 8.7.1 page 29.

Figure 12: HOLD feature

(The values shown above are for illustration purpose. Your meter may display different values depending on the settings & your environmental conditions)

Instruction Manual DO 300

7 MEMORY FUNCTIONS

7.1 Memory Input

Your meter stores data in sets that include:

z Dissolved oxygen with corresponding temperature

reading

z Time and date reading was taken

You can store up to 50 sets of data in any combination of %, mg/L or ppm readings.

To store a reading:

1. During any measurement function (MEAS), press the MI key to input any data into the memory.

2. MEM, “StO” and memory number will flash. The meter then returns to measurement mode.

NOTE: If the memory is full, the first value stored will be erased to create space for the new value.

Figure 15: Memory Input,

Third Value

Figure 13: Memory Input, First

Value

Figure 14: Memory Input,

Second Value

+ 42 hidden pages