Thermo Fisher Scientific DO 110 User Manual

Instruction Manual

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DO 110

Hand-held Dissolved Oxygen Meter with RS 232C

68X361305

Rev. 0 - 11/05

Technol o

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Preface

This manual serves to explain the use of the hand-held series meters. The model covered is the DO 110 hand-held meter.

The manual functions in two ways, firstly as a step by step guide to help the user operate the meter. Secondly, it serves as a handy reference guide.

This instruction manual is written to cover as many anticipated applications of the DO meter as possible. If there are doubts in the use of the DO110 meters, do not hesitate to contact the nearest Authorised Distributor.

It is recommended that all operators should read this manual prior to working with this instrument. Eutech Instruments / Oakton Instruments cannot accept any responsibility for damage or malfunction to the meter caused by improper use of the instrument.

The information presented in this manual is subject to change without notice as improvements are made, and does not represent a commitment on the part of Eutech Instruments Pte Ltd / Oakton Instruments.

Eutech Instruments Pte Ltd

Oakton Instruments

Rev. 0 - 11/05

TABLE OF CONTENTS

1 INTRODUCTION........................................................................................ ............1

2 DISPLAY & KEYPAD FUNCTIONS ...................................................................... 2

2.1 DISPLAY................................................................................................................................... 2

2.2 KEYPAD ................................................................................................................................... 3

3 PREPARATION ......................................................................................... ............4

3.1 INSERTING THE BATTERIES ........................................................................................................ 4

3.2 CONNECTING THE DO PROBE.................................................................................................... 4

3.3 CONNECTING THE AC/DC ADAPTER........................................................................................... 6

3.4 CONNECTING THE RS232C CABLE ............................................................................................ 6

3.4.1 RS232C Configuration..................................................................................................... 7

4 CALIBRATION............ ................................................ ...........................................8

4.1 IMPORTANT INFORMATION ON METER CALIBRATION..................................................................... 8

4.2 TEMPERATURE CALIBRATION ..................................................................................................... 9

4.3 DO CALIBRATION (WITH ATC) –% SATURATION MODE .............................................................. 10

4.3.1 To calibrate 100% Saturation: ........................... ..... ......... .... ..... ..... ......... .... ..... .... .......... 10

4.3.2 To calibrate 0% Saturation: .................................... .... ......... ..... ..... ......... .... ..... .... .......... 10

4.4 DO CALIBRATION IN MG/L (PPM) MODE..................................................................................... 11

5 MEASUREMENT ............................................ ..................................................... 12

5.1 AUTOMATIC TEMPERATURE COMPENSATION ............................................................................. 12

5.2 MANUAL TEMPERATURE COMPENSATION (MTC)....................................................................... 12

5.3 TAKING MEASUREMENTS......................................................................................................... 13

5.3.1 Taking measurements with READY indicator selected on... ..... ..... .... ......... ..... .... ..... ..... 13

5.3.2 Taking measurements with the AUTO HOLD feature selected on................................. 13

6 MEMORY FUNCTION..........................................................................................14

6.1 MEMORY INPUT ...................................................................................................................... 14

6.2 MEMORY RECALL ................................................................................................................... 14

7 PRINT FUNCTION ...............................................................................................15

7.1 USING DO 110 METER WITH PRINTER OR COMPUTER............................................................... 15

7.2 SENDING DATA TO COMPUTER OR PRINTER ............................................................................. 16

7.2.1 Print Current Data Manually.......................................................................................... 17

7.2.2 Print Data on Timed Interval.............................................................. ............................ 17

7.3 PRINT DATA FROM STORED MEMORY ....................................................................................... 18

8 ADVANCED SETUP FUNCTIONS......................................................................20

8.1 ADVANCED SETUP MODE OVERVIEW...................................................................................... 21

8.2 P1.0 : UNIT CONFIGURATION (COF) ........................................................................................ 23

8.2.1 P1.1: READY Indicator and Auto Hold function................................. ............................ 23

8.2.2 P1.2: Select °C or °F temperature units......................................................................... 24

8.2.3 P1.3: Selection of Automatic or Manual Temperature Compensation............................ 24

8.2.4 P1.4: Select mg/L or ppm mode.................................................................................... 25

8.3 P2.0: VIEWING PREVIOUS CALIBRATION DATA (CAL) ................................................................ 26

8.4 P3.0: VIEW ELECTRODE DATA (ELE)....................................................................................... 26

8.4.1 EL.1 Viewing the electrode Slope Factor....................................................................... 26

8.4.2 EL.2 Viewing the % Saturation Offset (Only available in % Saturation Set Up menu): .. 27

8.4.3 Viewing the 100% Saturation mV value......................................................................... 27

8.4.4 Viewing the 0% Saturation mV value............................................................................. 27

8.5 P4.0 : AUTO-OFF (ATO).......................................................................................................... 28

8.6 P5.0 : RESET TO FACTORY DEFAULT (RST) .............................................................................. 29

8.6.1 Calibration Reset.. ............................................................... .......................................... 29

8.6.2 User Reset.................................................................................................................... 30

8.7 P 6.0: MEMORY CLEAR (CLR)................................................................................................. 31

8.8 P7.0: DISSOLVED OXYGEN PARAMETERS (DPR)....................................................................... 32

8.8.1 Pressure Setting Adjustment...... ................................................................................... 32

8.8.2 Salinity Setting Adjustment (available in mg/L or ppm Set Up mode only).................... 32

8.9 P 8.0: % SATURATION OFFSET ADJUSTMENT (OFS) ................................................................. 33

9 CYBERCOMM PORTABLE DAS........................................................................34

9.1 SYSTEM REQUIREMENTS ......................................................................................................... 34

9.2 LOADING CYBERCOMM PORTABLE DAS ................................................................................... 34

9.3 RUNNING CYBERCOMM PORTABLE .......................................................................................... 41

i. Buttons & Check-Box ........................................................................................................... 42

ii. Menu............................................................................. ....................................................... 42

iii. Communication Settings............ .... ......... ..... ..... .... ......... ..... .... ..... ......... ..... .... ......... ..... ..... 43

9.4 CAPTURING AND PRINTING DATA INTO COMPUTER USING DATA ACQUISITION............................. 44

9.5 TROUBLE-SHOOTING GUIDE ..................................................................................................... 44

10 DISSOLVED OXYGEN PROBE ..................................................... ..................45

10.1 DISSOLVED OXYGEN PRINCIPLE ........................................................................................... 45

10.2 PROBE CARE ...................................................................................................................... 45

10.3 MEMBRANE HOUSING REPLACEMENT.................................................................................... 46

10.3.1 To replace the membrane cap (with pre-installed membrane) -.............. .... ..... .... ..... ..... 46

10.4 MEMBRANE/O-RING REPLACEMENT (OPTIONAL PROCEDURE)................................................. 47

10.5 ELECTROLYTE SOLUTION ..................................................................................................... 48

11 ERROR MESSAGES........................................................................................49

12 TROUBLE-SHOOTING.....................................................................................49

13 LIST OF ACCESSORIES..................................................................................51

13.1 REPLACEMENT METER AND METER ACCESSORIES ................................................................. 51

14 SPECIFICATIONS ............................................................................................52

15 WARRANTY......................................................................................................53

16 RETURN OF ITEMS......................................... ................................................. 53

Instruction Manual CyberScan DO 110

1 INTRODUCTION

Thank you for selecting the DO 110 meter. These meters are microprocessor-based instruments and are designed to be handy, capable of allowing one-hand operation. It is capable of measuring Dissolved Oxygen (DO) in mg/L or ppm units, % saturation and temperature. This meter has many user-friendly features – all of which are completely accessible through the water-resistant membrane keypad.

Please read this manual thoroughly before operating your meter.

Instruction Manual CyberScan DO 110

2 DISPLAY & KEYPAD FUNCTIONS

2.1 Display

The LCD has a primary and secondary display.

• The primary display shows the measured % Saturation or mg/L (or ppm) values.

• The secondary display shows the measured temperature.

The display also shows error messages, keypad functions and program functions.

Primary Display

123

SETUP

1. SETUP - Setup mode

indicator

2. MEAS - Measurement mode

indicator

3. CAL - Calibration indicator

4. MEM - Memory recall mode

indicator

READY HOLD ON OFF

MEAS CAL

-1.8.8.8

MEM ERR

-1.8.8.8

Secondar y D isplay

Figure 1: Active LCD screen

7. mg/l- milligram per liter

8. °C°F - Temperature

indicator

9. ATC - Automatic

Temperature Compensation indicator

10. ERR - Error indicator 16. HOLD – Hold indicator

MEM

ppm

mg/l

°C °F

ATC

910111213

13.

- Electrode indicator

14. indicator

15. ON – READY/Auto HOLD set

up enable indicator.

OFF – READY set up disable indicator

- Calibration standard

5. % - Percentage Saturation

of Oxygen

6. ppm – parts per million

11. MEM - Memory location

indicator

- Low battery indicator

17. READY – Ready indicator

Instruction Manual CyberScan DO 110

2.2 Keypad

A large membrane keypad makes the instrument easy to use. Some buttons have several functions depending on its mode of operation.

KEY

OFF

CAL

MEAS

HOLD

ENTER

SETUP

MODE

FUNCTION

• ON/OFF - Powers on and shuts off the meter. The meter will start in a measurement mode similar to the last measurement mode it is in before being switched off.

• CAL - Activates the DO % Saturation or mg/l calibration mode and when used with the MODE key, it activates the temperature calibration mode.

• MEAS - Allows return to measurement mode when canceling or terminating any operation.

• HOLD - Activates/Deactivates freezing of the measured reading while in measurement mode.

• ENTER - Confirms the calibration values in Calibration mode and the selection in SETUP and Print Function mode.

Scroll through the memory location and the stored data during memory recall.

• MI (Memory Input) - Captures the measured readings with its corresponding temperature values and stores them in the memory.

• MR (Memory Recall) - Retrieves the stored data from memory.

• ▲▼ - Sets the calibration values during mg/l or temperature calibration.

Scrolls through each SETUP and its sub group menu.

Scroll through Print Function menu and its parameters.

• SETUP - Activates the parameter setting menu to allow you to customise meter configuration, view calibration point and electrode data, select auto power off, reset meter, clear memory, set % Saturation Offset and set dissolved oxygen parameters. .

• MODE - Selects the measurement parameter option between % Saturation with temperature and mg/L (or ppm) with temperature.

• PRINT - Allows print of current measurement or stored data to either the printer or the computer.

Instruction Manual CyberScan DO 110

3 PREPARATION

3.1 Inserting the Batteries

This meter is packaged with 4 “AAA” alkaline batteries required for operation. To insert the batteries into the meter, follow the procedure outlined below.

1. To open the battery compartment, press down the catch of the battery cover. See below.

2. Note the polarity and insert the batteries into the battery compartment correctly

3. Replace the battery cover into its original position, ensuring the catch is locked in position.

Your hand-held meter is now ready for operation.

LR03 'AAA ' (AM4)

MADE IN S'PORE

Figure 3: Note battery's polarity

Figure 2: Open Battery Cover

3.2 Connecting the DO Probe

The DO 110 uses a special notched 6-pin connector to attach the probe to the meter.

Instruction Manual CyberScan DO 110

NOTE: Do not substitute other probes or electrodes. For replacement probe, see the “Accessories” section.

Keep connector dry and clean. Do not touch connector with soiled hands.

1. Line up the notch and 6 pins on the meter with the holes in the 6 pin connector. Push down and screw the metal sleeve to lock the probe connector into place.

2. To remove probe, unscrew the metal sleeve and slide up the probe connector. While holding onto metal sleeve, pull probe away from the meter.

CAUTION: Do not pull on the probe cord or the probe wires might disconnect.

Instruction Manual CyberScan DO 110

3.3 Connecting the AC/DC Adapter

Besides using four AAA-sized batteries as a power source, the DO 110 meter can also operate from the power mains using an AC/DC power adapter. This is extremely useful if you have an A.C. power source available (e.g. laboratory).

Before plugging in, switch off the meter and the power source of adapter. This is a safety precaution that should be adhered to safeguard your meter.

1. Switch off the meter and power sources.

2. Select the correct AC/DC Adapter which matches your input mains voltage.

3. Select the correct output voltage of the AC/DC adapter. (Output Voltage: 9 to 15 V DC, Current: >=50 mA).

4. Gently insert the power adapter D.C. jack into the meter power socket.

5. Switch on the power source of the adapter followed by the meter.

3.4 Connecting the RS232C Cable

The DO 110 meter provides a RS232C output for you to transmit your readings either to a printer or a computer via a cable. This is useful in instances where the meter is used for continuous monitoring of a certain process or experiment. Data output to the printer or the computer can then be evaluated.

The data is output in the ASCII format. This format allows the data to be imported by a wide variety of software that read ASCII data (e.g. Microsoft’s Excel, Lotus, Quattro-pro etc.). A complimentary Data Acquisition Software (DAS) is provided and it captures data transmitted into an ASCII file for later use.

Figure 4: Location of

RS232C

1. Open the printer port cover located at the bottom end of the meter. Do not use excessive force when doing this. See Figure 4.

2. Noting the orientation of the RS232C connector, plug the male connector into the RS232C port of the meter.

3. Fasten the RS232C connector by fastening the two screws at the side of the male RS232C connector.

Instruction Manual CyberScan DO 110

3.4.1 RS232C Configuration

The DO 110 meter has a 9 pin female RS232C connector with the following pin out:

PIN NO. DESCRIPTION

1 -

2 Transmit Data

3 -

4 -

5 CTS (Clear to Send)

6 -

7 GND (Ground)

8 -

9 -

A one-to-one connection can be made with a 9 pin RS232C port of the computer.

In case DO 110 meter’s output has to be sent to a 25 pin RS232C connector, the following cable configuration may be used:

DO 110 25 pin connector

2 (TxD) (RxD) 3

5 (CTS) (RTS) 4

7 (GND) (GND) 7

Instruction Manual CyberScan DO 110

4 CALIBRATION

4.1 Important Information on Meter Calibration

The amount of oxygen dissolved in water will depend on its temperature, atmospheric pressure and its salinity. While the pressure and salinity values are manually entered into the instrument, the temperature is being measured by the probe. It is therefore very important that the temperature is calibrated if necessary prior to the DO calibration.

The measurements of % Saturation of DO will linearly affect the measurement for DO in mg/L. Hence calibration in % Saturation of DO should be carried out first. This is described in the following section.

Before calibrating, press MODE key to select the correct measurement mode. There are 2 measurement modes for DO: mg/L or ppm, and % Saturation.

NOTE: All new calibrations will automatically over-ride existing calibration values.

Instruction Manual CyberScan DO 110

4.2 Temperature Calibration

DO in mg/L is dependent on temperature, so it is first necessary to calibrate or verify the temperature reading. The temperature sensor is part of the DO probe and the “ATC” annunciator will light up on the LCD screen once the probe is connected correctly to the meter.

1. Switch the meter on. The ATC annunciator will appear at the right-hand side of the LCD.

2. Press the CAL/MEAS key to enter calibration mode.

3. While in calibration mode, press the MODE key to enter temperature calibration mode. The primary display shows the measured temperature reading according to the last set offset

and the secondary display shows the

factory calibrated temperature measurement.

4. Dip the DO probe into a solution of known temperature (i.e. a temperature bath). Allow time for the temperature probe to stabilise.

5. Scroll with the MI/▲ or MR/▼ keys to set the correct temperature value (i.e. the temperature of the temperature bath). You can adjust the reading in increments of 0.1°C.

6. Once you have selected the correct temperature, press the HOLD/ENTER key. The meter automatically returns to the measurement mode.

NOTES: You can offset the temperature reading up to ±5°C from original reading.

To exit this program without confirming the temperature calibration value, DO NOT press ENTER. Press CAL/MEAS instead.

READY

MEAS

98.9

CAL

MEAS

CAL

83.4

100.0

MODE

22.3

22.0

HOLD

ENTER

MEAS

98.9

22.3

CAL

22.3

CAL

22.3

22.0

°C ATC

°C

MIMR

°C

ATC

°C ATC

It displays the value being measured currently, offset by the last calibration.

Instruction Manual CyberScan DO 110

4.3 DO Calibration (with ATC) –% Saturation Mode

Dissolved Oxygen exact calibration value depends on barometric pressure. The meter is set to a factory default of 760 mm Hg, which results in a calibration value of 100% saturation in air.

NOTE: If the barometric pressure setting has been changed from 760 mm Hg, the calibration value in air will automatically adjust to a value other than 100%. The adjusted value will be correct for the new barometric pressure setting. See Section

8.8.1 for barometric pressure setting.

4.3.1 To calibrate 100% Saturation:

1. Press MODE key to select % Saturation mode.

READY

MEAS

83.4

CAL

MEAS

CAL

25.3

°C ATC

83.4

2. Rinse the probe well with deionised rinse water. For

best accuracy, blot the end of the probe dry. Do not touch the membrane.

3. Hold the probe in the air gently with the sensor facing

down and press CAL key to calibrate the meter. The primary display will show the current value of measurement and the secondary display will show “100.0” (or the value corrected to its barometric pressure setting) to which the meter is going to be calibrated. Wait for the reading to stabilize.

READY

100.0

HOLD

ENTER

MEAS

100.0

25.3

°C ATC

4. Press ENTER key to confirm the calibration. The meter automatically

calibrates to 100% air saturation and returns to the measurement mode.

MEAS

4.3.2 To calibrate 0% Saturation:

1. Press MODE key to select % Saturation mode

2. Rinse the probe well with deionised rinse water.

3. Immerse the DO probe in 0% solution. Stir gently to

create a homogenous solution. Wait for the reading to stabilize.

4. Press CAL key to calibrate the meter. The primary

display will show the current value of measurement and the secondary display will show “0.0” to which the meter is going to be calibrated.

5. Press ENTER key to confirm the calibration. The meter

READY

3.4

CAL

MEAS

HOLD

ENTER

MEAS

25.3

CAL

3.4

0.0

automatically calibrates to 0% saturation and returns to the measurement mode.

NOTE: To exit from DO Calibration mode without confirming calibration, DO NOT press ENTER. Press CAL/MEAS instead

25.3

°C ATC

Instruction Manual CyberScan DO 110

4.4 DO Calibration in mg/L (ppm) Mode

MEAS

The amount of oxygen dissolved in a liquid will depend on its temperature, pressure and salinity. It is therefore very important to set these parameters correctly before attempting to do a

READY

8.10

24.5

calibration.

CAL

READY

MEAS

CAL

Temperature is measured by the meter automatically. Pressure and salinity values are keyed in manually as described in Sections 8.8.1 and 8.8.2.

8.10

1. From the measurement mode, press MODE key to select mg/L (ppm).

8.10

mg/l °C ATC

mg/l

°C

ATC

2. Rinse the probe well with deionised rinse water. For best accuracy, blot the end of the probe dry. Do not touch the membrane.

3. Dip the probe into a solution whose DO value is known.

4. Wait until the reading stabilizes. Press CAL key.

5. Adjust to the required value by pressing MI/S or MR/T key.

6. Press ENTER to confirm the calibration.

NOTE: To exit from DO Calibration mode without confirming calibration, press CAL/MEAS key.

READY

8.24

HOLD

ENTER

MEAS

8.24

CAL

8.10

24.5

mg/l °C ATC

mg/l °C

Instruction Manual CyberScan DO 110

5 MEASUREMENT

During measurement, care must be taken not to allow the membrane of the DO probe touch any surface. The probe can either be fully or partially immersed in the solution.

The READY indicator appears on the display when the readings stabilize. It will turn off if the readings start to fluctuate.

NOTE: It is important that the sample is stirred constantly to allow it to flow past the membrane for better readings.

5.1 Automatic Temperature Compensation

MEAS

98.9

The ATC indicator on the LCD indicates automatic temperature compensation (ATC).

5.2 Manual Temperature Compensation (MTC)

24.5

Figure 5: ATC indicator

°C ATC

For manual temperature compensation (MTC), you must set

CAL

the ATC mode in the SETUP menu Program P 1.3 to “NO”. See section 8.2.3. The [ATC] annunciator will disappear from

25.0

the LCD indicating manual temperature compensation mode.

25.0

1. Press the CAL/MEAS key to enter DO calibration mode.

2. While in DO calibration mode, press the MODE key to enter temperature calibration mode. The primary display act as the adjustable temperature setting and the secondary display shows the default temperature value of 25°C or indicates the last set temperature setting.

ENTER

3. Check the temperature of your sample using an accurate thermometer.

READY

4. Press the MI/▲ or MR/▼ keys to set the temperature to the measured value from step 3.

5. Press HOLD/ENTER key to confirm the selected temperature and to return to the measurement mode.

CAL

30.0

25.0

HOLD

MEAS

98.9

30.0

°C

MIMR

°C

6. The meter will now compensate DO readings for the manually set temperature.

NOTES: To exit this program without confirming the manual temperature

compensation value, DO NOT press ENTER in step 5. Press CAL/MEAS instead.

Instruction Manual CyberScan DO 110

5.3 Taking Measurements

1. Rinse the probe with deionised or distilled water before use to remove any

impurities adhering to the probe body. If it is dehydrated, soak it for 30 minutes in tap water.

2. Switch on the meter. The MEAS annunciator appears on the top of the

LCD. The ATC indicator appears in the lower right hand corner to indicate Automatic Temperature Compensation.

3. Dip the probe into the sample and allow some time for the reading to

stabilize

NOTE: When dipping the probe into the sample, make sure the tip of the probe is completely immersed. Stir the sample gently to create a homogenous sample. Be sure to tap probe very gently to remove air bubbles. Air bubbles will cause errors in the reading.

4. .When the reading is stable, the READY annunciator appears. Note the

reading on the display.

5. Press the HOLD key to freeze the value of the DO

reading for a delayed observation. Press the HOLD key again to release the held values.

6. To toggle between % Saturation and mg/l (ppm)

measurement mode, press the MODE key.

NOTE: This meter shuts off automatically after 20 minutes of last key press. If the meter is shut off either automatically or manually, the HOLD value will be lost. For longer storage, use the memory functions.

READY

MEAS

98.9

MODE

MEAS

8.24

24.5

°C ATC

mg/l °C ATC

5.3.1 Taking measurements with READY indicator selected on

If the READY indicator has been activated, the READY annunciator lights when the reading is stable. You can switch the READY indicator on or off in SETUP program. See 8.2.1 - P1.1: READY Indicator and Auto Hold function on page 23.

5.3.2 Taking measurements with the AUTO HOLD feature selected on

When a reading is stable for more than 5 seconds, the AUTO HOLD feature will automatically “HOLD” the reading. The “HOLD” indicator appears on the left side of the display. Press the HOLD/ENTER key to release the reading. Switch the Auto Hold feature on or off in SETUP program in section 8.2.1 - P1.1: READY Indicator and Auto Hold function on page 23.

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