Thermo Fisher Scientific CL750, iBright User Manual
iBright™ CL750 Imaging System
USER GUIDE
For use with the iBright™ CL750 Imaging System
Catalog Numbers A44116
Publication Number MAN0018652
Revision C.0
For Research Use Only. Not for use in diagnostic procedures.
Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0018652
Revision Date Description
C.0 24 August 2020 Adding SAE chapter due to addition of SAE instrument functionality.
•
Updated the components list (see Table 1 on page 6).
•
Added web link to interactive training demonstrations (see “Product description” on page 6).
•
B.0 23 September 2019
A.0 June 7 2019 New document. Describes installation, operation, and maintenance of the iBright™ Imaging Systems.
Updated the emission filters for imaging in Universal mode (see “Universal Mode” on page 16).
•
Added additional outputs for TPN-normalized data.
•
Added additional information for resetting factory defaults, backing up, and restoring settings (see
“Settings” on page 27).
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2020 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■
Product information ................................................................... 6
Product description ............................................................. 6
Contents ....................................................................... 6
■
Start, sign on, and configure the instrument ....................................... 8
Create an instrument profile ...................................................... 8
Create an instrument account and link to Connect using a cloud account .............. 8
Mobile device .............................................................. 9
PC using Connect account ................................................... 9
PC using instrument Connect app ............................................ 9
Use instrument to link to Connect account .................................... 10
Link to Connect using an existing instrument account .............................. 10
If the unit is o ................................................................. 10
If the unit is on and in sleep mode ................................................ 11
If the unit is on and in active mode ............................................... 11
■
iBright
Operate the iBright™ imager ........................................................ 12
Choose imaging mode and start image capture .................................... 12
Chemi Blots ................................................................... 13
Image using Chemi Blots ................................................... 13
Nucleic Acid Gels .............................................................. 14
Image using Nucleic Acid Gel ............................................... 14
Protein Gels ................................................................... 15
Image using Protein Gels ................................................... 15
Universal Mode ................................................................ 16
Options available in modes ...................................................... 17
Image Gallery .................................................................. 20
View images .............................................................. 20
™
CL750 Imaging System User Guide
3
Contents
■
■
Export and analyze images ......................................................... 21
Export images ................................................................. 21
Export new images ........................................................ 21
Export gallery images ...................................................... 21
Select an Export file type ................................................... 22
Archive/Backup g2i image files .............................................. 23
Analyze images ................................................................ 24
Analyze new images ....................................................... 24
Analyze gallery images ..................................................... 26
Maintain the instrument ............................................................. 27
Settings ....................................................................... 27
Manage accounts .......................................................... 29
Run instrument diagnostics ..................................................... 30
Clean the instrument ........................................................... 30
Replace the instrument fuses .................................................... 31
Materials required .......................................................... 31
Replace the fuses .......................................................... 31
Move the instrument ........................................................... 32
Prepare for shipping ....................................................... 33
■
Invitrogen™ iBright™ SAE Software Solution for 21 CFR Part 11 Support ..... 34
Security Audit e-Signature (SAE) for the iBright™ Imager ............................ 34
Activate your license key ........................................................ 35
Enable SAE mode .............................................................. 35
Disable SAE mode ............................................................. 36
Sign in to the instrument in SAE mode ............................................ 36
Change your password in SAE mode ............................................. 37
SAE user roles ................................................................. 37
SAE privileges for default user roles .............................................. 38
Non-SAE user roles ............................................................ 38
Acquire images in SAE mode .................................................... 39
Use the Gallery in SAE mode .................................................... 39
File status ................................................................ 39
Filter Gallery by file status .................................................. 39
Archive/Backup g2i image files in SAE mode ................................. 39
Commit changes ............................................................... 40
Sign data ..................................................................... 41
Unlock files ................................................................... 42
4
iBright™ CL750 Imaging System User Guide
Generate an audit history ....................................................... 42
View the e-Signature history ..................................................... 43
Export image files .............................................................. 43
Generate and export Analysis Report ............................................. 43
■
Instrument specifications ........................................................... 44
Instrument dimensions and specifications ......................................... 44
Electrical requirements ......................................................... 44
Environmental requirements ..................................................... 45
Network requirements .......................................................... 46
Instrument and computer connections ............................................ 47
■
Safety ................................................................................. 49
Symbols on this instrument ..................................................... 49
Safety alerts on this instrument .............................................. 50
Location of safety labels on the instrument .................................... 51
Safety information for instruments not manufactured by Thermo Fisher Scientific ...... 51
Instrument safety .............................................................. 51
General ................................................................... 51
Physical injury ............................................................. 52
LED (Light-Emitting Diode) .................................................. 52
Electrical safety ........................................................... 53
Cleaning and decontamination .............................................. 54
Instrument component and accessory disposal ................................ 54
Safety and electromagnetic compatibility (EMC) standards .......................... 55
Safety compliance ......................................................... 55
EMC ..................................................................... 55
Environmental design ...................................................... 56
Chemical safety ................................................................ 58
Biological hazard safety ......................................................... 59
Contents
■
Documentation and support ....................................................... 60
Related documentation and references ........................................... 60
Customer and technical support ................................................. 61
Limited product warranty ........................................................ 61
iBright™ CL750 Imaging System User Guide
5
IMPORTANT! Before using this product, read and understand the information in
the “Safety” appendix in this document.
Product description
The iBright™ CL750 Imaging System allows users to image chemiluminescent
Western blots, DNA and RNA gels stained with fluorescent nucleic acid stains, and
visible protein gels.
The iBright™ imager uses a simple design and intuitive workflows to deliver highresolution images. It can be run directly from the touchscreen to start and create
images, while also providing on-board software for image analysis. To further analyze
data, the instrument can be integrated with the iBright™ Image Analysis Software
available for desktop and Connect.
Product information
Contents
Go to thermofisher.com/ibrightcl750training for interactive visual demonstrations
of how to install, operate, and maintain your instrument.
Table 1 Included imager system parts
Component
iBright™ Imaging System 1
Power cord (region specific) 1
Quick Reference Guide 1
White transilluminator screen 1
iBright™ Imaging System Sample Blot 1
Sample stage/turntable 1
Product Information Sheet 1
Safe Imager™ Viewing Glasses 1
Quantity
6
iBright™ CL750 Imaging System User Guide
Product information
Contents
Table 2 Available accessories
Component Part number
White transilluminator screen A33828
Sample stage A33829
High-Power USB Wi-Fi Module A26774
iBright™ CL750 Imaging System User Guide
7
Start, sign on, and configure the
Create an instrument profile
instrument
1.
Touch
2.
Touch Get started.
3.
Touch Create a Profile.
4.
Enter a Screen name.
5.
Enter and confirm a four-digit pin.
6.
Touch Create profile.
7.
(Optional) If you want to link your instrument to your Connect account, see “Use
instrument to link to Connect account” on page 10 or “Link to Connect using
an existing instrument account” on page 10.
to sign in to your instrument user account.
Create an instrument account and link to Connect using a cloud account
1.
Touch
2.
Touch Get Started.
to sign in to your instrument user account.
3.
Click on Connect.
4.
Link your account using one of these methods:
•
Mobile device — Connect using a QR generated by the instrument and
scanned by the instrument Connect mobile app.
•
PC — Connect using a link code that is entered into the instrument Connect
app or your online Connect account.
•
Instrument — Enter your Connect account information directly on the
instrument.
8
iBright™ CL750 Imaging System User Guide
Mobile device
Start, sign on, and configure the instrument
Create an instrument account and link to Connect using a cloud account
1.
Touch Mobile Device.
2.
Launch instrument Connect mobile app.
3.
Sign into your Connect account on the mobile app.
4.
Click on
5.
Click on QR code.
6.
Scan QR code on the instrument.
Your Connect account should now be linked to your instrument account.
.
PC using Connect account
1.
Touch PC.
2.
Login to your Connect account online.
3.
Click on
4.
Click on .
5.
Select iBright from the drop-down menu.
6.
Enter the linking code and click on Send.
Your Connect account should now be linked to your instrument account.
.
PC using instrument Connect app
1.
Touch PC.
2.
iBright™ CL750 Imaging System User Guide
Launch the instrument Connect mobile app.
3.
Sign into your Connect account on the mobile app.
4.
Click on
5.
Click on Linking code.
6.
Enter the linking code and click Send.
Your Connect account should now be linked to your instrument account.
.
9
Start, sign on, and
Link to Connect using an existing instrument account
configure the instrument
Use instrument to link to Connect account
1.
Click on Instrument.
2.
Enter your Connect username and password.
3.
Click Link account.
Your Connect account should now be linked to your instrument account.
Link to Connect using an existing instrument account
Linking to Connect after account creation can only be done during the export
process.
1.
Click on .
2.
Select an image.
3.
Click on Actions.
If the unit is o
4.
Click on Export.
5.
Click on Choose destination.
6.
Click on Cloud.
7.
Click on Sign in.
8.
Connect using one of the methods outlined in “Create an instrument account
and link to Connect using a cloud account” on page 8.
1.
Ensure the unit is plugged in and turn on using the switch on the back of
instrument.
The instrument will go directly to the sign-in page.
2.
Enter a Screen name.
3.
Enter and confirm a 4-digit pin.
Note: For those not logged on as a user (guest), acquired images are saved to
a guest gallery. The guest gallery is not private. Any logged in user can view and
modify images residing in the guest gallery.
10
4.
From the Welcome screen, choose the appropriate Mode from the drop-down
menu.
iBright™ CL750 Imaging System User Guide
5.
Open drawer and place sample in the center of the stage.
6.
Close drawer. Touch Live View to see a live-view sample image.
If the unit is on and in sleep mode
1.
Touch the screen to wake.
Start, sign on, and
If the unit is on and in sleep mode
configure the instrument
2.
Touch
profile” on page 8 for instructions on how to create a user profile.
Note: For those not logged on as a user (guest), acquired images are saved
to a guest gallery. The guest gallery is not private. Any logged-in user or other
guest user can view and modify images residing in the guest gallery.
3.
Enter a Screen name.
4.
Enter and confirm a 4-digit pin.
5.
From the Welcome screen, choose the appropriate Mode from the drop-down
menu. Open drawer and place sample in the center of the stage.
6.
Close drawer. Touch Live View and a live-view sample image will be displayed
auto-zoomed and auto-focused.
to sign in to use or set up a user profile. See “Create an instrument
If the unit is on and in active mode
1.
If not already signed in, touch
“Create an instrument profile” on page 8 for instructions on how to create a user
profile.
to sign in to use or set up a user profile. See
Note: For those not logged on as a user (guest), acquired images are saved
to a guest gallery. The guest gallery is not private. Any logged-in user or other
guest user can view and modify images residing in the guest gallery.
2.
Enter and confirm a 4-digit pin.
3.
From the Welcome screen, choose the appropriate Mode from the drop-down
menu. Open drawer and place sample in the center of the glass platform.
4.
Close the drawer, then touch Live View and a live-view sample image will be
displayed auto-zoomed and auto-focused.
iBright™ CL750 Imaging System User Guide
11
Operate the iBright™ imager
Choose imaging mode and start image capture
On the Welcome screen:
1.
Use the drop-down menu to select the desired mode.
•
Chemi Blots to image chemiluminescent substrates.
•
Nucleic Acid Gels to image DNA and RNA gels.
•
Protein Gels to image visible, stained protein gels.
•
Universal to image samples containing multiple readouts, such as
chemiluminescence, colorimetric stains, and fluorescence (limited to green
transilluminator excitation 490-520 nm and emission 568-617 nm).
2.
Open drawer and place sample in the center of the viewing area.
3.
Close drawer. Touch Live View to see a live-view sample image.
Instrument automatically aligns and focuses on the sample.
Note: If any manual adjustments are required for zoom or focus, touch More
options4Camera and choose the appropriate menu item.
•
To image in Chemi Blots mode, proceed to “Image using Chemi Blots” on
page 13.
•
To image in Nucleic Acid Gels mode, proceed to “Image using Nucleic Acid Gel”
on page 14.
•
To image in Protein Gels mode, proceed to “Image using Protein Gels” on
page 15.
•
To image in Universal Mode, proceed to “Universal Mode” on page 16.
12
iBright™ CL750 Imaging System User Guide
Chemi Blots
Mode to image chemiluminescent Western blots.
Image using Chemi Blots
1.
Touch Smart Exposure.
The imager acquires a series of short exposures, then renders a preview image
and recommends an exposure time. This is not a real image.
2.
Touch More options4Region of Interest. Region of Interest is for directing
Smart Exposure to a specific region of the sample.
3.
(Optional) Adjust the exposure time using the following methods:
Operate the iBright
™
imager
Chemi Blots
•
Touch
•
Finger swipe in the segmented dial.
•
Touch the dial center box to select a preset exposure time or touch Custom
to manually input a time.
The image preview updates in real-time.
4.
Touch Enter (for a custom manual input).
5.
Touch Capture to acquire an image.
The captured image is displayed and automatically saves to the gallery.
6.
If an acceptable image, touch Export, Gallery, or Analyze. If an unacceptable
image, touch More options to optimize the image or touch Trash to remove the
image, then return to step 2 to adjust exposure conditions.
or .
iBright™ CL750 Imaging System User Guide
13
Operate the iBright
Nucleic Acid Gels
™
imager
Nucleic Acid Gels
Mode to image DNA and RNA gels stained with fluorescent nucleic acid stains such
as Ethidium Bromide, SYBR™ Safe, SYBR™ Green (I or II), SYBR™ Gold and similar
products.
Image using Nucleic Acid Gel
1.
Touch Smart exposure. Alternatively, to set a manual exposure time, touch the
center of the dial.
The imager acquires a series of short exposures and then renders a preview
image and a recommended exposure time. This is not a real image.
2.
Touch More options4Region of Interest. Region of Interest is for directing
Smart Exposure to a specific region of the sample.
3.
To adjust or set a manual exposure time, select a method:
•
Touch
•
Finger swipe in the segmented dial.
•
Touch the dial center box to select a preset exposure time or touch Custom
to manually input a time.
The image preview will update in real-time.
4.
Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
5.
If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to step 2 to adjust exposure conditions.
or within dial.
14
iBright™ CL750 Imaging System User Guide
Protein Gels
•
Protein Gels Visible: USE WHITE SCREEN BETWEEN SAMPLE AND
TRANSILLUMINATOR GLASS for samples including Coomassie-stained protein
gels and silver-stained protein gels.
•
Protein Gels Fluorescent: PLACE SAMPLE DIRECTLY ON
TRANSILLUMINATOR GLASS for samples including No-Stain™-lableled protein
gels and SYPRO™dye-stained protein gels.
Image using Protein Gels
1.
Select whether you are imaging a visible sample (Protein Gel Visible) or
fluorescent sample (Protein Gel Fluorescent).
2.
An auto-zoomed and focused Live View of the image is now displayed in the
viewport.
IMPORTANT! For visual stained protein on nitrocellulose and PVDF
membrane, use Universal Mode4Visible channel without the white screen.
Operate the iBright
™
imager
Protein Gels
3.
Touch Smart Exposure. Alternatively, to set a manual exposure time, touch the
center of the dial.
The imager acquires a series of short exposures and then renders a preview
image and a recommended exposure time. This is not a real image.
4.
Touch More options4Region of Interest. Region of Interest is for directing
Smart Exposure to a specific region of the sample. You can select dierent,
same, or no region of interest for each channel.
5.
To adjust or set a manual exposure time, select a method:
•
Touch or within dial.
•
Finger swipe in the segmented dial.
•
Touch the dial center box to select a preset exposure time or touch Custom
to manually input a time.
The image preview will update in real-time.
6.
Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
7.
If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to step 2 to adjust exposure conditions.
iBright™ CL750 Imaging System User Guide
15
™
Operate the iBright
Universal Mode
imager
Universal Mode
Mode to image samples containing one or more signal types (chemi, fluor, and/or
visible).
Table 3 Illumination source, excitation filter, and emission filter for the
iBright™ CL750 Instrument.
Illumination Excitation Filters Emission Filters
Green trans 490-520 nm 568-617 nm
1.
Touch Choose Channel and select from one to four dierent dyes or stains from
the pre-populated list.
To edit an existing dye, touch the dye name button. Touch Dye and change
a.
the assigned dye to any other pre-populated dye in the fluorescent dye
list. Dyes and stains in the pre-populated dye list are categorized by most
recently used, channel and name.
Touch False Color and change the false color assigned to that dye.
b.
Note: Each dye is assigned a false color to be used in the composite
overlay.
Touch Exposure Time and manually enter the desired exposure time.
c.
Note: Smart Exposure is recommended (see step 2).
To remove this assigned dye from the channel, touch Remove Dye at the
d.
bottom left hand corner of the screen and then Confirm.
Channel refers to the type of signal you want to capture and the imaging
conditions you want to use.
•
Chemi: Channel for measuring chemiluminescent signals.
•
Fluor [Trans]: Channel for measuring fluorescent signals that are
excited by the green transilluminator and appropriate emission filter.
•
Nucleic acid: Channel for measuring fluorescent signals in gel that are
excited by the green transilluminator and the 568-617 emission filter
(stained DNA and RNA).
•
Protein: Channel for measuring fluorescent and visible signals in gel
that are excited or illuminated by the green transilluminator and the
568-617 emission filter — stained or labeled protein.
•
Visible: Channel for measuring visible signals on an opaque media
(nitrocellulose membrane, PVDF membrane, etc.) using the Epi-white
LED with neutral density filter and appropriate emission filter.
•
TPN: Total protein normalization channel is for normalizing Western
blot results to total protein load by lane.
16
iBright™ CL750 Imaging System User Guide
Operate the iBright™ imager
Options available in modes
2.
Touch Smart Exposure. Alternatively, for manual exposure, touch More
options4Manual Exposure.
The imager will acquire a series of short exposures for each dye type selected.
It will then render preview images and a recommended exposure time for each
channel. These are not real images.
3.
To adjust or set a manual exposure time for any of the channels that you are
acquiring, select the channel (dye) that you want to change:
•
Touch
•
Finger swipe in the segmented dial.
•
Touch the dial center box to select a preset exposure time or touch Custom
to manually input a time.
Note: When recommended exposure time is adjusted, the image preview will
update in real time.
4.
Touch Capture to acquire all of the selected channels with the indicated
exposure times. Captured image(s) appears in viewport and automatically saves
to the gallery.
5.
If an acceptable image, touch Export, Gallery, or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to step 3 to adjust exposure conditions.
or within dial.
Options available in modes
Each mode oers More Options on certain workflow screens to provide detailed
camera and image adjustment.
Table 4 Camera
Suboption
Resolution/Sensitivity
Zoom/Focus
iBright™ CL750 Imaging System User Guide
Detail
Changes binning setting to increase resolution/decrease
sensitivity or decrease resolution/increase sensitivity.
Binning levels: 1×1, 2×2, 4×4.
Changes zoom level to increase or decrease image area.
Optimizes focus for sharpness.
Note: Digital zoom 1X to 2X.
17
Operate the iBright™ imager
Options available in modes
Table 5 Routines
Suboption Detail
Multi-exposure
Signal accumulation
Table 6 Image adjust
Suboption
Contrast
Series of 5 preset exposure times producing one image for
each time period.
Series of exposures where user defines the first capture
(time interval), the last capture (cumulative capture time)
and the total number of captures.
Note: Images acquired using Signal Accumulation are
not recommended for publication or analysis. This feature
is recommended for determining an optimal exposure
time.
Detail
Grayscale dierentiation between image features. Can use
Auto contrast (high, medium, low), or view raw image
with no contrast. User can manually adjust Gamma,
White, and Black slider bars.
Allow user to navigate between individual channels
associated with a common image file; pertains only to
Channels and Layers
Zoom
Invert
Saturation
Chemi and Universal modes (e.g., Channels: membrane,
No-Stain™, chemi, Composite). Layers allow toggling on
and o analysis tools.
Increases or decreases digital zoom to select an area of
the image.
Produces a negative or positive (white to black or black to
white) of the displayed image.
False-colors saturated white pixels (65,536) as red to
dierentiate from non-saturated pixels.
Table 7 Other options
Suboption
Region of Interest
Manual exposure Manually select an exposure time for sample.
Specify an area on the image to determine the optimal
exposure time for that region.
Detail
18
iBright™ CL750 Imaging System User Guide
Table 7 Other options (continued)
Suboption Detail
Predicts optimal exposure for minimizing pixel saturation
Smart exposure
and maximizing dynamic range for a specific sample.
Renders a preview of how the image will appear at the
recommended exposure time.
Operate the iBright™ imager
Options available in modes
Band excision (Nucleic
Acid Gels mode)
Membrane overlay
(Chemi Blots mode)
2UP view (Universal Mode)
Provides toggle button to turn on/o the LED light to allow
for band excision on nucleic acid gels.
Allows visible membrane overlay to see any visible
prestained markers that aid in identifying molecular
weights for unknown samples.
After a Smart Exposure or Capture, images are
displayed by default in a 2UP view.
•
Top image displays individual channels in grayscale.
Switch between individual channels by touching the
channel you want to display and edit. After a dye is
selected, that channel image is displayed in the top
view and becomes editable so the user can increase
or decrease the exposure time dial and see in real
time the eect on the image preview.
•
Bottom image displays the color composite with
channels displayed as dierent false colors overlaid
on a black background. Individual channels can
be toggled on and o in the composite image by
touching the eyeball at the left of the appropriate
channel name.
•
For the Visible channel color composite, the
signal is false‑colored overlaid on a white
background. Example: This channel is used to display
Coomassie‑stained protein gels.
1UP view (Universal Blot
Auto-enhance
iBright™ CL750 Imaging System User Guide
mode)
After a Smart Exposure or Capture, images can also be
displayed in a 1UP view.
Image displays the grayscale and false-color composite
images by toggling Gray or Color. User can switch
between individual channels by touching the dye name
you want to display. Individual channels can be toggled on
and o in the composite image by touching
on the left
of the appropriate dye name.
Note: Functionality for toggling between individual
channels in grayscale view or toggling on and o individual
channels in the false-color composite view is identical to
the 2UP view.
Removes the channel level background to enhance the
displayed image.
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