MINI VERTICAL GEL SYSTEM
1
TABLE OF CONTENTS
Intended Uses ..............................................................................2
Components Provided......................................................................2
Unpacking and Setting Up the System.....................................................2
Overview of the Assembly ..................................................................3
Preparations for Gel Casting: Assembly of the Glass Plate Cassettes .....................3
Gel Casting Using the Casting Stand .......................................................6
Removal of the Comb ......................................................................7
Setting up Gels in the Outer Tank ..........................................................7
Sample Loading and Electrophoresis ......................................................9
Disassembling the Glass Plate Cassettes.................................................10
Cleaning and Decontamination Guidelines ...............................................10
Buffers and Reagents Most Commonly Used for SDS-PAGE............................10
Guidelines for the Use of the Buffers and Reagents for SDS-PAGE .....................12
Replacement Parts and Additional Optional Accessories ................................12
Appendix A. Relationships Between Volts, Milliamps, Watts and Chamber
Appendix A. or Gel Resistance ...........................................................14
Appendix B. Running Multiple Chambers................................................15
Appendix C. Use of Precast Gels.........................................................15
Appendix D. References..................................................................16
Warranty .................................................................Inside Back Cover
MINI VERTICAL GEL SYSTEM
16
Ausubel, S.M., Brent, R., Kingston, R.R., Moore, D.D., Seidman, J.G., Smith, J.A., and
Struhl, K. 1987. Current Protocols in Molecular Biology. John Wiley & Sons, New York, NY.
Hames, B.D., and Rickwood, D. 1990. Gel Electrophoresis of Proteins: A Practical
Approach, 2nd Edition. Oxford University Press, Oxford, United Kingdom.
Rickwood, D. and Hames, B.D., 1990. Gel Electrophoresis of Nucleic Acids: A Practical
Approach, 2nd Edition. Oxford University Press, Oxford, United Kingdom.
Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory
Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
TABLE IV
Source Name & Description Clamps
ISS SepraGels or Use thin precast clamps
Daiichi Multigels (EC132B).
(with notched glass plates;
gels are 1 mm thick)
Jule Jule Gels Use thin precast clamps
Daiichi Multigels (EC132B).
(with notched glass plates;
gels are 0.75 mm or
1.5 mm thick)
Jule Snap-a-Gels Use standard clamps (EC132).
(plastic cassettes with a
notch; gels are 1 mm thick)
Novex
™
Pre-Cast Gels Use wide clamps (EC132A).
(plastic cassettes with a
notch; gels are 1 mm or
1.5 mm thick)
Bio–Rad Ready Gel Use standard clamps (EC132).
Precast Gels
Owl PAGE-ONE™ GELS Use standard clamps (EC132).
SEPRA GELS
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