Thermo IEC CL31 User manual

IEC CL31 - CL31R Multispeed

User’s Manual

Analyze • Detect • Measure • Control

TM

IEC CL31 - CL31R Multispeed

User’s Manual

IEC CL31 - CL31R Multispeed

Carefully read this manual before operating your instrument.

Information contained in this document is the property of Thermo Electron Corporation; it may not be
duplicated or distribuited without the owner’s authorization.

The validity of the guarantee is subject to the observation of the instructions
and precautions described in this document.

MANUAL NUMBER

REV ECR/ECN DATE DESCRIPTION BY

a 11290087 02/06 Initial release

Preface

Thermo Electron Corporation

IEC CL31 - CL31R i

Packing List

 11210913 Centrifuge IEC CL31 230V 50/60 Hz
 11210914 Centrifuge IEC CL31 120V 60 Hz
 11210915 Centrifuge IEC CL31 100V 50/60 Hz

 11210917 Centrifuge IEC CL31R 230V 50/60 Hz
 11210918 Centrifuge IEC CL31R 120V 60 Hz
 11210919 Centrifuge IEC CL31R 100V 50 Hz
 11210920 Centrifuge IEC CL31R 100V 60 Hz

Item number Quantity Check

Centrifuge 1 

11290087 User manual 1 

Manual lid opening instructions 1 

64227001 Mains cord 1 

Toolbox:

11202979 Rotor removal tool 1 

25535010 Emergency unlocking tool 2 

26387032 Spare fuses (CL31 only) 1 

For missing parts call your nearest Thermo representative

ii IEC IEC CL31 - CL31R Thermo Electron Corporation

WEEE Compliance

Great Britain

Deutschland

WEEE Compliance. This products is required to comply with the

European Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2002/96EC. It is marked with the following symbol. Thermo
Electron has contracted with one or more recycling/disposal companies
in each EU Member State, and this products should be disposed of or
recycling through them. Further information on Thermo Electron’s
compliance with these Directives, the recyclers in your country, and
information on Thermo Electron products which may assist the detec­tion of substances subject to the RoHS Directive are available at

www.thermo.com/WEEERoHS

WEEE Konformittät. Dieses Produkt muss die EU Waste Electrical &
Electronic Equipment (WEEE) Richtlinie 2002/96ECerfüllen. Das
Produkt ist durch folgendes Symbol gekennzeichnet. Thermo Electron
hat Vereinbarungen getroffen mit Verwertungs-/Entsorgungsanlagen in
allen EU-Mitgliederstaaten und dieses Produkt muss durch diese
Firmen widerverwetet oder entsorgt werden. Mehr Informationen über
die Einhaltung dieser Anweisungen durch Thermo Electron, die
Verwerter und Hinweise die Ihnen nützlich sein können, die Thermo
Electron Produkte zu identizfizieren, die unter diese RoHS Anweisung
fallen, finden Sie unter www.thermo.com/WEEERoHS

Italia

Espana

Conformità WEEE. Questo prodotto deve rispondere alla direttiva

dell’Unione Europea 2002/96EC in merito ai Rifiuti degli Apparecchi
Elettrici ed Elettronici (WEEE). È marcato col seguente simbolo.
Thermo Electron ha stipulato contratti con una o diverse società di
riciclaggio/smaltimento in ognuno degli Stati Membri Europei. Questo
prodotto verrà smaltito o riciclato tramite queste medesime. Ulteriori
informazioni sulla conformità di Thermo Electron con queste
Direttive, l’elenco delle ditte di riciclaggio nel Vostro paese e informa­zioni sui prodotti Thermo che possono essere utili alla rilevazione di
sostanze soggette alla Direttiva RoHS sono disponibili sul sito

www.thermo.com/WEEERoHS

WEEE Conformidad. Este producto requiere cumplir con la Directiva
2002/96EC WEEE (residuos de quipos electricos y electronicos). Este
producto esta marcado con el simbolo WEEE, como se describe. En
cada Estado Miembro de la Union Europea (EU), Thermo Electron ha
contratado con diversas companias para disponer y/o re-ciclar los resi­duos de equipos electricos y electronicos. Existe information adictional
acerca de la conformidad de Thermo Electron con esta Directiva,
incluyendo los nombres de las diversas companias autorizadas para dis­poner/recyclar nuestros productos. Adicionalmente suministramos

IEC CL31 - CL31R iiiThermo Electron Corporation

WEEE Compliance

informacion acerca de los productos de Thermo Electron que puedes
asistir en la deteccion de substancian mensionades en la directiva
RoHS, explicado en www.thermo.com/WEEERoHS

France

Conformité WEEE. Ce produit doit être conforme à la directive euro-

péenne (2002/96EC) des Déchets d’Equipements Electriques et
Electroniques (DEEE). Il est merqué par le symbole suivant. Thermo
Electron s’est associé avec une ou plusieurs compagnies de recyclage
dans chaque état membre de l’union européenne et ce produit devrait
être collecté ou recyclé par celles-ci. Davantage d’informations sur la
conformité de Thermo Electron à ces directives, les recycleurs dans
votre pays et les informations sur les produits Thermo Electron qui
peuvent aider le détection des substances sujettes à la directive RoHS
sont disponibles sur www.thermo.com/WEEERoHS

iv IEC CL31 - CL31R Thermo Electron Corporation

Declaration of compliance

Declaration of compliance

Declaration de conformite

Konformitätserklärung

Declaración de conformidad

Declaraçao de conformidade

We, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, declare that the instrument defined
here below complies with the following European directives:

Nous, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne
53200 CHATEAU-GONTIER, France, déclarons que le matériel dési­gné ci-après est conforme aux directives européennes suivantes:

Wir, Thermo Electron Industries S.A.S. , Z.I. d'Aze Bellitourne 53200,
CHATEAU-GONTIER, France, erklären dass das hier beschriebene
Gerät sich in Übereinstimmung mit folgenden europäischen
Richtlinien befindet:

Nostros, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne
53200 CHATEAU-GONTIER, France, declaramos que el instrumen­to bajo descrito está fabricado según las especificaciones establecidas
por las siguientes normas europeas:

Nós, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, declaramos que o instrumento aqui
apresentado está de acordo com as normas européias abaixo listadas:

Dichiarazione di conformità

Noi, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, dichiariamo che lo strumento sotto
descritto è conforme alle seguenti Direttive europee:

• Machinery: 98 / 37 / CEE

• Low voltage: 72 / 23 / CEE - 93 / 68 CEE

• EMC: 89 / 336 CEE - 92 / 31 CEE - 93 / 68 CEE

• Safety standard: EN 61010-1 - EN 61010-2-020

Château-Gontier, 28th June 2005

Managing Director
Christian Galiana

IEC CL31 - CL31R vThermo Electron Corporation

Guarantee Terms

Thermo guarantees that this unit is free from defects in materials and
workmanship when it leaves the factory, and will replace or repair the
unit if it proves defective in normal use or during service for a period of
ONE YEAR from the delivery.

This guarantee is invalid if the unit is incorrectly used, poorly serviced
or neglected, mis-used or accidentally damaged.

There is no explicit guarantee other than as stated above.

vi IEC CL31 - CL31R Thermo Electron Corporation

For further information,
assistance or service

United States of America

Tel.: [phone]
Tel.: [phone]

Canada or Latin America

Tel.: [phone]
Fax: [phone]

China (Shanghai)

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India (Mumbai)

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Japan

Tel.: [phone]
Fax:+ 81 454 53 9222

Other Asia-Pacific Countries

Tel.: [phone]

Italy

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Netherlands

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Russia/Cis

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Spain and Portugal

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Switzerland

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United Kingdom and Ireland

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Other Europe, Middle East, or Africa

Tel.: [phone]
Tel.: [phone]

Austria

Tel.: [phone]

France

Tel.: [phone]
[phone]

Belgium

Tel.: [phone]

Germany

Tel.: [phone]
Fax: [phone]

Finland

Tel.: [phone]

In Other Countries:

Contact your local THERMO products
distributor or agent.

E-mail Technical Service Representative for
products at:

[e-mail]

Visit our web site at: www.thermo.com

IEC CL31 - CL31R viiThermo Electron Corporation

Contents

Chapter 1

Chapter 2

Chapter 3

Use and Function........................................................................................1-1

User Manual ....................................................................................1-1

Warning Symbols..............................................................................1-1

Description ......................................................................................1-1

Refrigeration and Heating Systems....................................................1-2

Drive System ....................................................................................1-3

Safety Interlock System ....................................................................1-3

Imbalance Detection System ............................................................1-3

Relative Centrifugal Force ................................................................1-4

Specifications............................................................................................ 2-1

Dimensions and Weight....................................................................2-1

Electrical Specifications ....................................................................2-1

Performance ......................................................................................2-1

Installation.................................................................................................. 3-1

Environmental Conditions................................................................3-1

Unpacking ........................................................................................3-1

Positioning........................................................................................3-2

Mains Supply ....................................................................................3-2

Lid Opening and Rotor Checking ....................................................3-2

Manual Lid Unlocking Procedure ....................................................3-3

Performance and Accessories ............................................................3-5

Chapter 4

Chapter 5

viii IEC CL31 - CL31R Thermo Electron Corporation

Instructions for Use....................................................................................4-1

Controls and Indicators ....................................................................4-1

Display Screens ................................................................................4-2

Control Panel Functions ..................................................................4-2

Control Panel Indicators ..................................................................4-3

Description of Certain Events with ..................................................4-4

Preparing the First Run of the Day ..................................................4-5

Sample Loading ................................................................................4-5

Hazards, Precautions and Limitations of Use ......................................5-1

IEC 1010-2-020................................................................................5-1

Cautions............................................................................................5-1

Speed Control ..................................................................................5-2

Operational Limitations ....................................................................5-2

Aerosol Risks ....................................................................................5-2

Mechanical Risks ..............................................................................5-3

Contents

Chapter 6

Service and Maintenance ........................................................................6-1

Periodic Cleaning..............................................................................6-1

Disinfection ......................................................................................6-1

Radioactive Decontamination ..........................................................6-2

Power Supply Circuit Breaker ..........................................................6-2

Rotor Removal..................................................................................6-2

Trunnion Lubrication ......................................................................6-3

Chemical Compatibility Table for Rotors,

Tubes and Accessories ......................................................................6-4

IEC CL31 - CL31R ixThermo Electron Corporation

Chapter 1 Use and Function

User Manual

Warning Symbols

The user manual is part of the centrifuge, and contains important
information for your safety and for the best use of the equipment

Always keep the manual close to the appliance and in a safe place,
so that it is always available. Thermo strongly recommends that all
users read this manual carefully.

The following symbols are provided to help the operator take
advantage of the protection afforded by the equipment and to warn of
potential danger.

On the main switch, allows centrifuge to operate. ▲

On the main switch, disconnects the centrifuge from the main power
supply. ▲

Only authorized personnel can touch the parts close to this symbol
and, in any case, only after switching off the main power supply.

Coming in contact with high voltages could cause severe injuries.

▲

Description

In this manual, this symbol means that you will find important
information for safety, which if unobserved could result in damage to
the appliance and/or harm to the operator. ▲

In this manual, this symbol means that you will find important
information about minimizing biological risk: if unobserved the
result could be harm to the operator. ▲

The CL31 and CL31R centrifuges are designed for laboratory use.

They will separate the components of fluids into layers of varying
density by subjecting them to high forces.

Centrifugal force provided by the centrifuge can also be used to drive
solvents and low molecular weight solutes through the membrane of a
filtering device. Retained macrosolutes will therefore be found above
the membrane.

IEC CL31 - CL31R 1-1Thermo Electron Corporation

Chapter 1

Use and Function

Refrigeration Systems

Relative Centrifugal Force (RCF) generated by a rotor is directly
proportional to its sedimentation useful radius and to the value of its
speed squared.

A control system permits the user, through an extremely intuitive
control panel, to set and control the speed, the g-force, the temperature
(on the thermostated and refrigerated versions) and the run time, as
well as to view different messages and warnings.

The CL31 is a ventilated unit. A permanent air circulation reduces the
heat level in the bowl.

However the temperature in the bowl can reach 10 °C above the
ambient temperature after hours of continuous operation.

The CL31R is equipped with a powerful refrigeration system that allows
samples to be processed at low temperature. The refrigerant, R134a, is
free of CFC (Chloro-Fluoro-Carbons) in accordance with the Montreal
Protocol directions for preservation of the ozone layer in the atmosphere.

Condensation drain on

refrigerated units

The actual temperature in the bowl is continuously displayed on the
front panel of the centrifuge. Under certain conditions (high speed,
long duration runs) the sample temperature can be higher than the
bowl temperature on the display. In this case, the user may compensate
manually after empirical determination of the temperature differential.

The lid should remain closed when the refrigeration system is in use to
avoid condensation.

The centrifuge has a rubber hose that allows condensation to be
drained from the bowl. The drain hose exits at the back of the
machine. Near the drain hose you can find the following label:

To remove condensation, please follow this procedure:

• Switch off the centrifuge and disconnect the power.

• Put a small basin under the hose.

• Unplug the hose.

1-2 IEC CL31 - CL31R Thermo Electron Corporation

Drive System

Safety Interlock

System

Chapter 1

Use and Function

• Drain the centrifuge bowl.

• Insert the plug back into the hose.

Attention Do not remove the plug from the drain hose during
centrifugation. ▲

A three phase brushless motor drives the rotating equipment. The rotor
is contained in a sealed, armor plated centrifugation chamber.

The brushless motor requires no maintenance. A control system
ensures the correct drive speed, which is continuously monitored.

The CL31 and CL31R centrifuges are equipped with an interlock
system that assures two basic safety features:

• The run cannot be started if the lid is not correctly closed.

• The centrifuge lid cannot be opened if the rotor is running.

Current safety regulations allow the centrifuge lid opening when rotor
speed is below a certain defined level (very few rpm). This conditions is
therefore not to be considered a fault or riscky for the operator. ▲

The centrifuge will not operate until the lid is closed and latched in
place.

The lid remains latched until the rotor stops spinning. The lid access
lamp indicates when the rotor has stopped: consequently the handle on
the right hand side of the centrifuge can be used to open the lid.

If a power failure occurs, access to the samples in the centrifuge is
possible via a manual lid opening procedure (see appropriate
paragraph). For this operation a special tool (supplied with the
centrifuge) is required.

Imbalance Detection

System

Bypass the interlock system only under emergency conditions as the
rotor could still be rotating. ▲

The CL31 centrifuge is equipped with a load imbalance detector.

In case of excessive imbalance, the load imbalance indicator LED is
illuminated and the brake is applied immediately. The rotor will be

IEC CL31 - CL31R 1-3Thermo Electron Corporation

Chapter 1

Use and Function

Relative Centrifugal

Force

decelerated to rest in few seconds.
As soon as the motor stops, open the centrifuge and redistribute the
samples to produce an equal weight on diametrically opposite sides.

If the message persists, despite your efforts to balance the load, call
your Thermo Service representative.

Note Imbalance tolerance depends upon the rotor in use. The
centrifuge will tolerate 10 gr. of imbalance with the 4 x 280 mL rotor
equipped with standard buckets. Carefully balance the sample load to
avoid actuating the imbalance detection system. ▲

Relative Centrifugal Force (RCF), at the circumference of a rotor and
bucket combination, is directly proportional to the speed (r.p.m.) and
radius of the rotor. Therefore, a greater r.p.m. and/or a larger radius
produces a greater RCF and improved faster separation of substances.
The centrifuge control system carries out and displays the results of all
calculations related to speed, radius and RCF.

Configuration formule

Note The value introduced for the radius can be adjusted to allow for

position within the tube such as at a boundary. Maximum radii are
quoted in the specifications tables. Use of improper radius will adjust
the speed setting, automatically applying the wrong RCF. ▲

Legend

R = radius (in millimetres)

N = speed (in r.p.m.) ÷ 1000

RCF = gravitational acceleration ‘g’

M+ = add to memory

MR = memory recall

Note To calculate actual results, press the keys on a pocket calculator in
the order shown. ▲

1-4 IEC CL31 - CL31R Thermo Electron Corporation