Thermo Heraeus Pico 17, Heraeus Pico 21 User manual

Thermo Scientific
Heraeus Pico 17 / 21
Instruction Manual
50133388-2 September 2012
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T
Table of Contents
Preface .......................................................................................................................................iii
Scope of Supply ....................................................................................................................... iii
Intended Use ..............................................................................................................................iv
Accident Prevention .................................................................................................................iv
Precautions .................................................................................................................................v
Chapter 1 Introduction and Description ............................................................................................... 1-1
Characteristics of the Heraeus Pico 17 / 21 .............................................................. 1-2
Technical Data ........................................................................................................ 1-2
Directives, Standards and Guidelines ...................................................................................... 1-4
Functions and Features ............................................................................................................ 1-4
Mains Supply ........................................................................................................... 1-5
Rotor Selection ........................................................................................................ 1-5
Chapter 2 Before Use ............................................................................................................................... 2-1
Before Setting up ..................................................................................................... 2-2
Transporting the Centrifuge .................................................................................... 2-2
Location .................................................................................................................. 2-2
Leveling the Centrifuge ............................................................................................ 2-3
Mains Connection ................................................................................................... 2-3
Storage ..................................................................................................................... 2-4
Chapter 3 Control Panel .......................................................................................................................... 3-1
Control Panel .......................................................................................................... 3-2
Keys ......................................................................................................................... 3-2
Chapter 4 Operation ................................................................................................................................. 4-1
Switch on Centrifuge ............................................................................................... 4-2
Lid Opening ............................................................................................................ 4-2
Close Door .............................................................................................................. 4-2
Rotor Installation ..................................................................................................... 4-2
Entering Parameters ................................................................................................. 4-4
Centrifugation ......................................................................................................... 4-6
Short-term Centrifugation ....................................................................................... 4-7
Removing the Rotor ................................................................................................ 4-8
Aerosol-tight Rotors ................................................................................................. 4-8
Audible Alarm ......................................................................................................... 4-8
Turning off the Centrifuge ...................................................................................... 4-9
Chapter 5 Maintenance and Care ......................................................................................................... 5-1
Thermo Scientific Heraeus Pico 17 / 21 i
Inhalt
Cleaning Intervals .................................................................................................... 5-2
Cleaning .................................................................................................................. 5-2
Disinfection ............................................................................................................. 5-3
Decontamination ..................................................................................................... 5-4
Autoclaving .............................................................................................................. 5-5
Service of Thermo Fisher Scientific .......................................................................... 5-5
Shipping and Deposing of Accessories ..................................................................... 5-6
Chapter 6 Troubleshooting ..................................................................................................................... 6-1
Mechanical Emergency Door Release ...................................................................... 6-2
Troubleshooting by User ......................................................................................... 6-3
When to contact a Service Technician ..................................................................... 6-6
Chemical Compatibility Chart ...............................................................................A-1
Contact Information ................................................................................................B-1
ii Heraeus Pico 17 / 21 Thermo Scientific
P
Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty.
Scope of Supply
Article Number Quantity Check
Centrifuge Heraeus Pico 17 / 21 1
Power supply cable 1
Rotor 1
20360104 Square box wrench 1
50134706 CD with Manual 1
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
This symbol refers to general hazards. WARNING means that injuries or material damage or contamination could occur. CAUTION means that material damage could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol refers to hot surfaces. Observe the information contained in the instruction manual to keep yourself and your environment safe.
Thermo Scientific Heraeus Pico 17 / 21 iii
P Intended Use
g
cm
3
---------- -
Intended Use
This centrifuge is a laboratory product designed to separate components by generation of a
relative centrifugal force. It separates human samples (e.g. blood, urine and other body fluids) collected in appropriate containers, either alone or after addition of reagents or other additives.
As general-purpose centrifuge, it is designed to also run other containers filled with chemicals,
environmental samples and other non-human body samples.
Together with the hematocrit rotor 75003473 the centrifuge can be used as an in-vitro
diagnostics device to define the hematocrit value by centrifugation. Apply DIN 58933 to define the hematocrit value.
The centrifuge is to be used for separating materials of different density or particle size
suspended in a liquid.
Maximum sample density at maximum speed: 1.2
Accident Prevention
WARNING If a hazardous situation occurs, turn off the power supply to the centrifuge
and leave the area immediately.
WARNING In case of rotor failure the centrifuge can be damaged. The coolant can escape. Ventilate the room well and leave it. Inform customer service.
WARNING Plug the centrifuge only into sockets which have been properly grounded.
Prerequisite for the safe operation of the Heraeus Pico 17 / 21 is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user.
The safety regulations contain the following basic recommendations:
Maintain a radius of at least 30 cm around the centrifuge.
Implementation of special measures which ensure that no one can approach the
centrifuge for longer than absolutely necessary while it is running.
The mains plug must be freely accessible at all times. Pull out the power supply plug or
disconnect the power supply in an emergency.
iv Heraeus Pico 17 / 21 Thermo Scientific
Precautions
P Precautions
In order to ensure safe operation of the Heraeus Fresco 17 / 21, the following general safety regulations must be followed:
The centrifuge should be operated by trained specialists only.
The centrifuge is to be used for its intended use only.
While handling centrifuge, rotor, and samples you must wear laboratory clothing (e.g.
gloves).
Do not move the centrifuge while it is running.
Do not lean on the centrifuge.
Use only rotors and accessories for this centrifuge which have been approved by Thermo
Fisher Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the RCF value of the rotor.
Do not use rotors which show any signs of corrosion and/or cracks.
Do not touch the mechanical components of the rotor and do not make any changes to
the mechanical components.
Use only with rotors which have been properly installed. Follow the instructions in
section “Rotor Installation” on page 4-2.
Use only with rotors which have been loaded properly. Follow the instructions given in
the rotor manual.
Never overload the rotor. Follow the instructions given in the rotor manual.
Never open the lid until the rotor has come to a complete stop and this has been
confirmed in the display.
The lid emergency release may be used in emergencies only to recover the samples from
the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door
Release” on page 6-2).
Never use the centrifuge if parts of its cover panels are damaged or missing.
Do not touch the electronic components of the centrifuge or alter any electronic or
mechanical components.
Please observe the safety instructions.
Thermo Scientific Heraeus Pico 17 / 21 v
P Precautions
Remaining risk: Improper use can cause damages, contamination, and inju­rieswith fatal consequences.
Remaining risk: Improper use can cause damages, contamination, and inju­rieswith fatal consequences.
Please pay particular attention to the following aspects:
Location: well-ventilated environment, set-up on a level and rigid surface with adequate
load-bearing capacity.
Rotor installation: make sure the rotor is locked properly into place before operating the
centrifuge.
Especially when working with corrosive samples (salt solutions, acids, bases), the
accessory parts and vessel have to be cleaned carefully.
Always balance the samples.
Centrifuging hazardous substances:
Do not centrifuge explosive or flammable materials or substances which could react
violently with one another.
The centrifuge is neither inert nor protected against explosion. Never use the centrifuge
in an explosion-prone environment.
Do not centrifuge inflammable substances.
Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms
without suitable safety precautions.
When centrifuging microbiological samples from the Risk Group II (according to the Bio-safety Manual" of the World Health Organization (WHO)), aerosol-tight biological seals have to be used.
For materials in a higher risk group, extra safety measures have to be taken.
If toxins or pathogenic substances have gotten into the centrifuge or its parts,
appropriate disinfection measures have to be taken (see “Disinfection” on page 5-3).
Highly corrosive substances which can cause material damage and impair the
mechanical stability of the rotor, should only be centrifuged in corresponding protective tubes.
vi Heraeus Pico 17 / 21 Thermo Scientific
1
Introduction and Description
Contents
“Characteristics of the Heraeus Pico 17 / 21” on page 1-2
“Technical Data” on page 1-2
“Directives, Standards and Guidelines” on page 1-4
“Functions and Features” on page 1-4
“Mains Supply” on page 1-5
“Rotor Selection” on page 1-5
Thermo Scientific Heraeus Pico 17 / 21 1-1
1 Introduction and Description
Characteristics of the Heraeus Pico 17 / 21
Characteristics of the Heraeus Pico 17 / 21
Several rotors with a wide range of tubes can be used.
The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and low-vibration operation even at high speeds, and guarantees a very long lifetime.
The user-friendly control panel makes it easy to pre-set the speed, RCF value, run time, and temperature. You can choose between the display of speed and RCF or the entry mode.
These settings can be changed even while the centrifuge is running.
With the help of the key , you can also centrifuge a sample for just a few seconds, if called for.
The Heraeus Pico 17 / 21 is equipped with various safety features:
The housing is made of impact-resistant plastic and the interior of armor steel.
The door is equipped with a view port and a lock.
The centrifuge door can only be opened while the centrifuge is switched on and the rotor has come
to a complete stop. The centrifuge cannot be started until the door has been closed properly.
Door emergency release: For emergencies only, e.g. during power failures (see “Mechanical
Emergency Door Release” on page 6-2)
Technical Data
The technical data of the Heraeus Pico 17 / 21 is listed in the following table.
Table 1-1. Technical data Heraeus Pico 17
Feature Value
Environmental Conditions -Use in interior Spaces
Permissible Ambient Temperature during Operation
Permissible Ambient Temperature during Storage and Transport
-Altitudes of up to 2,000 m above Sea Level
-Max. relative Humidity 80% up to 31°C; decreasing linearly up to 50% relative Humidity at 40°C.
+5°C to +40°C +5°C to +40°C +5°C to +40°C
-10°C to +50°C -10°C to +50°C -10°C to +50°C
Overvoltage Category II II II
Pollution Degree 2 2 2
Heat Dissipation Heraeus Pico 17 Heraeus Pico 17 Heraeus Pico 17
230 V 120 V 100 V
614 BTU/h 614 BTU/h 580 BTU/h
IP 20 20 20
Run Time unlimited unlimited unlimited
1-2 Heraeus Pico 17 / 21 Thermo Scientific
Table 1- 1. Technical data Heraeus Pico 17
Feature Value
1 Introduction and Description
Technical Data
Max Speed n
Min Speed n
max
min
Maximum RCF-Value at n
max
13 300 rpm (depending on the Rotor)
13 300 rpm (depending on the Rotor)
13 300 rpm (depending on the Rotor)
300 rpm 300 rpm 300 rpm
17,000 x g 17,000 x g 17,000 x g
Maximum Kinetic Energy <1.90 kNm <1.90 kNm <1.90 kNm
Noise Level at maximum Speed < 56 dB (A) < 56 dB (A) < 56 dB (A)
Dimensions
Height Width Depth
230 mm 240 mm 350 mm
230 mm 240 mm 350 mm
230 mm 240 mm 350 mm
Weight with emptyRotor 10.5 kg 10.5 kg 10.5 kg
Table 1- 2. Technical data Heraeus Pico 21
Feature Value
Environmental Conditions -Use in interior Spaces
-Altitudes of up to 2,000 m above Sea Level
-max. relative humidity 80% up to 31°C; decreasing linearly up to 50% relative humidity at 40°C.
Permissible Ambient Temperature during
+5°C to +40°C +5°C to +40°C +5°C to +40°C
Operation
Permissible Ambient Temperature during
-10°C to +50°C -10°C to +50°C -10°C to +50°C
Storage and Transport
Overvoltage Category II II II
Pollution Degree 2 2 2
Heat Dissipation Heraeus Pico 21 Heraeus Pico 21 Heraeus Pico 21
230 V 120 V 100 V
785 BTU/h 751 BTU/h 785 BTU/h
IP 20 20 20
Run Time unlimited unlimited unlimited
Max Speed n
Min Speed n
max
min
Maximum RCF-Value at n
max
14 800 rpm (depending on the Rotor)
14 800 rpm (depending on the Rotor)
14 800 rpm (depending on the Rotor)
300 rpm 300 rpm 300 rpm
21,100 x g 21,100 x g 21,100 x g
Maximum Kinetic Energy <2.35 kNm <2.35 kNm <2.35 kNm
Noise level at maximum Speed < 56 dB (A) < 56 dB (A) < 56 dB (A)
Dimensions
Thermo Scientific Heraeus Pico 17 / 21 1-3
1 Introduction and Description
Directives, Standards and Guidelines
Table 1-2. Technical data Heraeus Pico 21
Feature Value
Height Width Depth
Weight with empty Rotor 10.5 kg 10.5 kg 10.5 kg
230 mm 240 mm 350 mm
Directives, Standards and Guidelines
Table 1-3. Directives, Standards and Guidelines
Tension / Frequency
230V 50Hz/60Hz,
100V 50Hz cCSAus: UL Std. No. 61010-1 (2
Marking
CE:
98/79/EC In-vitro-Diagnostika (IvD)
2006/42/EC Machine Directive:
2004/108/EC EMC Directive
2006/95/EC Low Voltage Directive:
Produced and inspected according to the following standards and guidelines
EN 61010-1, 2
EN 61010-2-020, 2
EN 61010-2-101
EN 61326-1
EN 61326-2-6
EN 55011B
EN 61000-6-2
EN ISO 13485
/CSA-C22.2 No. 61010-1, -04nd Edition
CAN/CSA-C22.2 No. 61010-2-020-09 Part 2-020:
IEC61010-2-020 (2
(Pollution degree 2, Overvoltage category II)
IEC 61010-2-101
nd
Edition
nd
Edition
nd
nd
Edition)
230 mm 240 mm 350 mm
Edition)
230 mm 240 mm 350 mm
Functions and Features
The following table gives an overview of the important functional and performance characteristics of the Heraeus Pico 17 / 21.
Table 1-4. Functions and Features
Component / Function Description / Features
Structure / Housing Sheet Metal with attached Plastic Housing and Steel Chamber
Rotor Chamber Plastic
Up to 48 ml of spilled Liquid is retained in the Chamber and cannot enter the Instrument.
Drive Induction Drive without Carbon Brushes
Keys and Display Easy-to-clean Keypad and Display Surface
Control Microprocessor-controlled
Internal Memory The most recent Data is saved
1-4 Heraeus Pico 17 / 21 Thermo Scientific
Table 1- 4. Functions and Features
Component / Function Description / Features
Functions RCF, Temperature, and pre-temp Selection
Door Lock Automatic Door Closing and Locking starting from an initial hold Position
Mains Supply
The following table contains an overview of the electrical connection data for the Heraeus Pico 17 / 21. This data is to be taken into consideration when selecting the mains connection socket.
Table 1-5 . Electrical connection data
1 Introduction and Description
Mains Supply
Cat.
75002410 PICO 17 230 V 50 / 60 Hz 1.4 A 180 W 4 A, 2-pole circuit
75002411 PICO 17 120 V 60 Hz 2.6 A 180 W 8 A, 2-pole circuit
75002412 PICO 17 100 V 60 Hz 2.9 A 170 W 8 A, 2-pole circuit
75002415 PICO 21 230 V 50 / 60 Hz 1.7 A 230 W 4 A, 2-pole circuit
75002416 PICO 21 120 V 60 Hz 3.4 A 220 W 8 A, 2-pole circuit
75002417 PICO 21 100 V 60 Hz 3.9 A 230 W 8 A, 2-pole circuit
Mains Voltage
Frequency
Rated Current
Power Consumption
Equipment Fuse
breaker
breaker
breaker
breaker
breaker
breaker
Rotor Selection
The Heraeus Pico 17 / 21 is is supplied with a rotor.
Various rotors are available to choose from.
Building Fuse
16 AT
15 AT
16 AT
16 AT
15 AT
16 AT
Microliter 24 x 2 7500 3424
Microliter 36 x 0.5 7500 3436
Dual Rotor 18 x 2 / 0.5 7500 3418
PCR Rotor 4x8 7500 3440
PCR Rotor 8x8 7500 3489
Hematocrit Rotor 7500 3473
The technical data of the rotors and the corresponding adapters and reduction sleeves for various commercially available containers can be found in the corresponding rotor operating manuals.
For more information visit our website at: http://www.thermo.com
Thermo Scientific Heraeus Pico 17 / 21 1-5
2
Before Use
Contents
“Before Setting up” on page 2-2
“Transporting the Centrifuge” on page 2-2
“Location” on page 2-2
“Leveling the Centrifuge” on page 2-3
“Mains Connection” on page 2-3
“Storage” on page 2-4
Thermo Scientific Heraeus Pico 17 / 21 2-1
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