Thermo Heraeus Pico 17, Heraeus Pico 21 User manual

Thermo Scientific

Heraeus Pico 17 / 21

Instruction Manual

50133388-2 September 2012

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T

Table of Contents

Preface .......................................................................................................................................iii

Scope of Supply ....................................................................................................................... iii

Intended Use ..............................................................................................................................iv

Accident Prevention .................................................................................................................iv

Precautions .................................................................................................................................v

Chapter 1 Introduction and Description ............................................................................................... 1-1

Characteristics of the Heraeus Pico 17 / 21 .............................................................. 1-2

Technical Data ........................................................................................................ 1-2

Directives, Standards and Guidelines ...................................................................................... 1-4

Functions and Features ............................................................................................................ 1-4

Mains Supply ........................................................................................................... 1-5

Rotor Selection ........................................................................................................ 1-5

Chapter 2 Before Use ............................................................................................................................... 2-1

Before Setting up ..................................................................................................... 2-2

Transporting the Centrifuge .................................................................................... 2-2

Location .................................................................................................................. 2-2

Leveling the Centrifuge ............................................................................................ 2-3

Mains Connection ................................................................................................... 2-3

Storage ..................................................................................................................... 2-4

Chapter 3 Control Panel .......................................................................................................................... 3-1

Control Panel .......................................................................................................... 3-2

Keys ......................................................................................................................... 3-2

Chapter 4 Operation ................................................................................................................................. 4-1

Switch on Centrifuge ............................................................................................... 4-2

Lid Opening ............................................................................................................ 4-2

Close Door .............................................................................................................. 4-2

Rotor Installation ..................................................................................................... 4-2

Entering Parameters ................................................................................................. 4-4

Centrifugation ......................................................................................................... 4-6

Short-term Centrifugation ....................................................................................... 4-7

Removing the Rotor ................................................................................................ 4-8

Aerosol-tight Rotors ................................................................................................. 4-8

Audible Alarm ......................................................................................................... 4-8

Turning off the Centrifuge ...................................................................................... 4-9

Chapter 5 Maintenance and Care ......................................................................................................... 5-1

Thermo Scientific Heraeus Pico 17 / 21 i

Inhalt

Cleaning Intervals .................................................................................................... 5-2

Cleaning .................................................................................................................. 5-2

Disinfection ............................................................................................................. 5-3

Decontamination ..................................................................................................... 5-4

Autoclaving .............................................................................................................. 5-5

Service of Thermo Fisher Scientific .......................................................................... 5-5

Shipping and Deposing of Accessories ..................................................................... 5-6

Chapter 6 Troubleshooting ..................................................................................................................... 6-1

Mechanical Emergency Door Release ...................................................................... 6-2

Troubleshooting by User ......................................................................................... 6-3

When to contact a Service Technician ..................................................................... 6-6

Chemical Compatibility Chart ...............................................................................A-1

Contact Information ................................................................................................B-1

ii Heraeus Pico 17 / 21 Thermo Scientific

P

Preface

Before starting to use the centrifuge, read through this instruction manual carefully and follow the
instructions.

The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.

Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty.

Scope of Supply

Article Number Quantity Check

Centrifuge Heraeus Pico 17 / 21 1

Power supply cable 1

Rotor 1

20360104 Square box wrench 1

50134706 CD with Manual 1

If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.

This symbol refers to general hazards.
WARNING means that injuries or material damage or contamination could occur.
CAUTION means that material damage could occur.

This symbol refers to biological hazards.

Observe the information contained in the instruction manual to keep yourself and your
environment safe.

This symbol refers to hot surfaces.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

Thermo Scientific Heraeus Pico 17 / 21 iii

P Intended Use

g

cm

3

---------- -

Intended Use

• This centrifuge is a laboratory product designed to separate components by generation of a

relative centrifugal force. It separates human samples (e.g. blood, urine and other body fluids)
collected in appropriate containers, either alone or after addition of reagents or other additives.

• As general-purpose centrifuge, it is designed to also run other containers filled with chemicals,

environmental samples and other non-human body samples.

• Together with the hematocrit rotor 75003473 the centrifuge can be used as an in-vitro

diagnostics device to define the hematocrit value by centrifugation. Apply DIN 58933 to define
the hematocrit value.

• The centrifuge is to be used for separating materials of different density or particle size

suspended in a liquid.

Maximum sample density at maximum speed: 1.2

Accident Prevention

WARNING If a hazardous situation occurs, turn off the power supply to the centrifuge

and leave the area immediately.

WARNING In case of rotor failure the centrifuge can be damaged. The coolant can
escape. Ventilate the room well and leave it. Inform customer service.

WARNING Plug the centrifuge only into sockets which have been properly grounded.

Prerequisite for the safe operation of the Heraeus Pico 17 / 21 is a work environment in
compliance with standards, directives and trade association safety regulations and proper
instruction of the user.

The safety regulations contain the following basic recommendations:

• Maintain a radius of at least 30 cm around the centrifuge.

• Implementation of special measures which ensure that no one can approach the

centrifuge for longer than absolutely necessary while it is running.

• The mains plug must be freely accessible at all times. Pull out the power supply plug or

disconnect the power supply in an emergency.

iv Heraeus Pico 17 / 21 Thermo Scientific

Precautions

P Precautions

In order to ensure safe operation of the Heraeus Fresco 17 / 21, the following general safety
regulations must be followed:

• The centrifuge should be operated by trained specialists only.

• The centrifuge is to be used for its intended use only.

• While handling centrifuge, rotor, and samples you must wear laboratory clothing (e.g.

gloves).

• Do not move the centrifuge while it is running.

• Do not lean on the centrifuge.

• Use only rotors and accessories for this centrifuge which have been approved by Thermo

Fisher Scientific. Exceptions to this rule are commercially available glass or plastic
centrifuge tubes, provided they have been approved for the speed or the RCF value of
the rotor.

• Do not use rotors which show any signs of corrosion and/or cracks.

• Do not touch the mechanical components of the rotor and do not make any changes to

the mechanical components.

• Use only with rotors which have been properly installed. Follow the instructions in

section “Rotor Installation” on page 4-2.

• Use only with rotors which have been loaded properly. Follow the instructions given in

the rotor manual.

• Never overload the rotor. Follow the instructions given in the rotor manual.

• Never open the lid until the rotor has come to a complete stop and this has been

confirmed in the display.

• The lid emergency release may be used in emergencies only to recover the samples from

the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door

Release” on page 6-2).

• Never use the centrifuge if parts of its cover panels are damaged or missing.

• Do not touch the electronic components of the centrifuge or alter any electronic or

mechanical components.

• Please observe the safety instructions.

Thermo Scientific Heraeus Pico 17 / 21 v

P Precautions

Remaining risk: Improper use can cause damages, contamination, and inju­rieswith fatal consequences.

Remaining risk: Improper use can cause damages, contamination, and inju­rieswith fatal consequences.

Please pay particular attention to the following aspects:

• Location: well-ventilated environment, set-up on a level and rigid surface with adequate

load-bearing capacity.

• Rotor installation: make sure the rotor is locked properly into place before operating the

centrifuge.

• Especially when working with corrosive samples (salt solutions, acids, bases), the

accessory parts and vessel have to be cleaned carefully.

• Always balance the samples.

Centrifuging hazardous substances:

• Do not centrifuge explosive or flammable materials or substances which could react

violently with one another.

• The centrifuge is neither inert nor protected against explosion. Never use the centrifuge

in an explosion-prone environment.

• Do not centrifuge inflammable substances.

• Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms

without suitable safety precautions.

When centrifuging microbiological samples from the Risk Group II (according to the
Bio-safety Manual" of the World Health Organization (WHO)), aerosol-tight biological
seals have to be used.

For materials in a higher risk group, extra safety measures have to be taken.

• If toxins or pathogenic substances have gotten into the centrifuge or its parts,

appropriate disinfection measures have to be taken (see “Disinfection” on page 5-3).

• Highly corrosive substances which can cause material damage and impair the

mechanical stability of the rotor, should only be centrifuged in corresponding protective
tubes.

vi Heraeus Pico 17 / 21 Thermo Scientific

1

Introduction and Description

Contents

• “Characteristics of the Heraeus Pico 17 / 21” on page 1-2

• “Technical Data” on page 1-2

• “Directives, Standards and Guidelines” on page 1-4

• “Functions and Features” on page 1-4

• “Mains Supply” on page 1-5

• “Rotor Selection” on page 1-5

Thermo Scientific Heraeus Pico 17 / 21 1-1

1 Introduction and Description

Characteristics of the Heraeus Pico 17 / 21

Characteristics of the Heraeus Pico 17 / 21

Several rotors with a wide range of tubes can be used.

The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and
low-vibration operation even at high speeds, and guarantees a very long lifetime.

The user-friendly control panel makes it easy to pre-set the speed, RCF value, run time, and
temperature. You can choose between the display of speed and RCF or the entry mode.

These settings can be changed even while the centrifuge is running.

With the help of the key , you can also centrifuge a sample for just a few seconds, if called for.

The Heraeus Pico 17 / 21 is equipped with various safety features:

• The housing is made of impact-resistant plastic and the interior of armor steel.

• The door is equipped with a view port and a lock.

• The centrifuge door can only be opened while the centrifuge is switched on and the rotor has come

to a complete stop. The centrifuge cannot be started until the door has been closed properly.

• Door emergency release: For emergencies only, e.g. during power failures (see “Mechanical

Emergency Door Release” on page 6-2)

Technical Data

The technical data of the Heraeus Pico 17 / 21 is listed in the following table.

Table 1-1. Technical data Heraeus Pico 17

Feature Value

Environmental Conditions -Use in interior Spaces

Permissible Ambient Temperature during
Operation

Permissible Ambient Temperature during
Storage and Transport

-Altitudes of up to 2,000 m above Sea Level

-Max. relative Humidity 80% up to 31°C; decreasing linearly up to
50% relative Humidity at 40°C.

+5°C to +40°C +5°C to +40°C +5°C to +40°C

-10°C to +50°C -10°C to +50°C -10°C to +50°C

Overvoltage Category II II II

Pollution Degree 2 2 2

Heat Dissipation Heraeus Pico 17 Heraeus Pico 17 Heraeus Pico 17

230 V 120 V 100 V

614 BTU/h 614 BTU/h 580 BTU/h

IP 20 20 20

Run Time unlimited unlimited unlimited

1-2 Heraeus Pico 17 / 21 Thermo Scientific

Table 1- 1. Technical data Heraeus Pico 17

Feature Value

1 Introduction and Description

Technical Data

Max Speed n

Min Speed n

max

min

Maximum RCF-Value at n

max

13 300 rpm
(depending on the
Rotor)

13 300 rpm
(depending on the
Rotor)

13 300 rpm
(depending on the
Rotor)

300 rpm 300 rpm 300 rpm

17,000 x g 17,000 x g 17,000 x g

Maximum Kinetic Energy <1.90 kNm <1.90 kNm <1.90 kNm

Noise Level at maximum Speed < 56 dB (A) < 56 dB (A) < 56 dB (A)

Dimensions

Height
Width
Depth

230 mm
240 mm
350 mm

230 mm
240 mm
350 mm

230 mm
240 mm
350 mm

Weight with emptyRotor 10.5 kg 10.5 kg 10.5 kg

Table 1- 2. Technical data Heraeus Pico 21

Feature Value

Environmental Conditions -Use in interior Spaces

-Altitudes of up to 2,000 m above Sea Level

-max. relative humidity 80% up to 31°C; decreasing linearly up to
50% relative humidity at 40°C.

Permissible Ambient Temperature during

+5°C to +40°C +5°C to +40°C +5°C to +40°C

Operation

Permissible Ambient Temperature during

-10°C to +50°C -10°C to +50°C -10°C to +50°C

Storage and Transport

Overvoltage Category II II II

Pollution Degree 2 2 2

Heat Dissipation Heraeus Pico 21 Heraeus Pico 21 Heraeus Pico 21

230 V 120 V 100 V

785 BTU/h 751 BTU/h 785 BTU/h

IP 20 20 20

Run Time unlimited unlimited unlimited

Max Speed n

Min Speed n

max

min

Maximum RCF-Value at n

max

14 800 rpm
(depending on the
Rotor)

14 800 rpm
(depending on the
Rotor)

14 800 rpm
(depending on the
Rotor)

300 rpm 300 rpm 300 rpm

21,100 x g 21,100 x g 21,100 x g

Maximum Kinetic Energy <2.35 kNm <2.35 kNm <2.35 kNm

Noise level at maximum Speed < 56 dB (A) < 56 dB (A) < 56 dB (A)

Dimensions

Thermo Scientific Heraeus Pico 17 / 21 1-3

1 Introduction and Description

Directives, Standards and Guidelines

Table 1-2. Technical data Heraeus Pico 21

Feature Value

Height
Width
Depth

Weight with empty Rotor 10.5 kg 10.5 kg 10.5 kg

230 mm
240 mm
350 mm

Directives, Standards and Guidelines

Table 1-3. Directives, Standards and Guidelines

Tension /
Frequency

230V
50Hz/60Hz,

100V 50Hz cCSAus: UL Std. No. 61010-1 (2

Marking

CE:

98/79/EC In-vitro-Diagnostika (IvD)

2006/42/EC Machine Directive:

2004/108/EC EMC Directive

2006/95/EC Low Voltage Directive:

Produced and inspected according to the following standards
and guidelines

EN 61010-1, 2

EN 61010-2-020, 2

EN 61010-2-101

EN 61326-1

EN 61326-2-6

EN 55011B

EN 61000-6-2

EN ISO 13485

/CSA-C22.2 No. 61010-1, -04nd Edition

CAN/CSA-C22.2 No. 61010-2-020-09 Part 2-020:

IEC61010-2-020 (2

(Pollution degree 2, Overvoltage category II)

IEC 61010-2-101

nd

Edition

nd

Edition

nd

nd

Edition)

230 mm
240 mm
350 mm

Edition)

230 mm
240 mm
350 mm

Functions and Features

The following table gives an overview of the important functional and performance characteristics of
the Heraeus Pico 17 / 21.

Table 1-4. Functions and Features

Component / Function Description / Features

Structure / Housing Sheet Metal with attached Plastic Housing and Steel Chamber

Rotor Chamber Plastic

Up to 48 ml of spilled Liquid is retained in the Chamber and cannot enter the
Instrument.

Drive Induction Drive without Carbon Brushes

Keys and Display Easy-to-clean Keypad and Display Surface

Control Microprocessor-controlled

Internal Memory The most recent Data is saved

1-4 Heraeus Pico 17 / 21 Thermo Scientific

Table 1- 4. Functions and Features

Component / Function Description / Features

Functions RCF, Temperature, and pre-temp Selection

Door Lock Automatic Door Closing and Locking starting from an initial hold Position

Mains Supply

The following table contains an overview of the electrical connection data for the Heraeus Pico 17 / 21.
This data is to be taken into consideration when selecting the mains connection socket.

Table 1-5 . Electrical connection data

1 Introduction and Description

Mains Supply

Cat.

75002410 PICO 17 230 V 50 / 60 Hz 1.4 A 180 W 4 A, 2-pole circuit

75002411 PICO 17 120 V 60 Hz 2.6 A 180 W 8 A, 2-pole circuit

75002412 PICO 17 100 V 60 Hz 2.9 A 170 W 8 A, 2-pole circuit

75002415 PICO 21 230 V 50 / 60 Hz 1.7 A 230 W 4 A, 2-pole circuit

75002416 PICO 21 120 V 60 Hz 3.4 A 220 W 8 A, 2-pole circuit

75002417 PICO 21 100 V 60 Hz 3.9 A 230 W 8 A, 2-pole circuit

Mains
Voltage

Frequency

Rated
Current

Power
Consumption

Equipment Fuse

breaker

breaker

breaker

breaker

breaker

breaker

Rotor Selection

The Heraeus Pico 17 / 21 is is supplied with a rotor.

Various rotors are available to choose from.

Building
Fuse

16 AT

15 AT

16 AT

16 AT

15 AT

16 AT

Microliter 24 x 2 7500 3424

Microliter 36 x 0.5 7500 3436

Dual Rotor 18 x 2 / 0.5 7500 3418

PCR Rotor 4x8 7500 3440

PCR Rotor 8x8 7500 3489

Hematocrit Rotor 7500 3473

The technical data of the rotors and the corresponding adapters and reduction sleeves for various
commercially available containers can be found in the corresponding rotor operating manuals.

For more information visit our website at: http://www.thermo.com

Thermo Scientific Heraeus Pico 17 / 21 1-5

2

Before Use

Contents

• “Before Setting up” on page 2-2

• “Transporting the Centrifuge” on page 2-2

• “Location” on page 2-2

• “Leveling the Centrifuge” on page 2-3

• “Mains Connection” on page 2-3

• “Storage” on page 2-4

Thermo Scientific Heraeus Pico 17 / 21 2-1

2 Before Use

Before Setting up

Before Setting up

1. Check the centrifuge and the packaging for any shipping damage.
Inform the shipping company and Thermo Fisher Scientific immediately if any damage is
discovered.

2. Remove the packaging.

3. Remove the rotor transport protection. The cardboard is suppose to prevent damage due to
impact.

4. Check the order for completeness (see “Scope of Supply” on page iii).
If the order is incomplete, please contact Thermo Fisher Scientific.

Transporting the Centrifuge

WARNING Always lift the centrifuge on both sides. Never lift the centrifuge by its front

or the back panel.
Transport the centrifuge if possible in its packaging and with the rotor transport
protection.

Location

• Due to its weight (see “Technical Data” on page 1-2), the centrifuge should be carried by several

people.

• Always lift the centrifuge at both sides on the bottom plate.

Figure 2-1.

• The centrifuge can be damaged by impacts.

The centrifuge should only be operated indoors.

Lifting the centrifuge at both sides.

The set-up location must fulfill the following requirements:

• A safety zone of at least 30 cm must be maintained around the centrifuge.

People and hazardous substances must be kept out of the safety zone while centrifuging.

• The supporting structure must be stable and free of resonance, for example a level laboratory

bench.

• The supporting structure must be suitable for horizontal setup of the centrifuge.

2-2 Heraeus Pico 17 / 21 Thermo Scientific

• The location must be free of grease and dust.

Main Power Switch

Mains plug

• The centrifuge should not be exposed to heat and strong sunlight.

WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.

• The set-up location must be well-ventilated at all times.

Leveling the Centrifuge

The horizontal leveling of the centrifuge must be checked every time after moving it to a different
location.
The supporting structure must be suitable for horizontal setup of the centrifuge.

CAUTION If the centrifuge is not level, imbalances can occur and the centrifuge can be
damaged.
Do not place anything under the centrifuge to level.

2 Before Use

Leveling the Centrifuge

Mains Connection

Figure 2-2. Mains Connection

1. Turn off the power supply switch on the back (press "0").

2. Plug the centrifuge into grounded electrical sockets only.

Note The centrifuge must be connected directly to the socket. Extension cords and multiple

sockets are not allowed.

3. Check whether the cable complies with the safety standards of your country.

Thermo Scientific Heraeus Pico 17 / 21 2-3

2 Before Use

Storage

Storage

4. Make sure that the voltage and frequency correspond to the figures on the rating plate.
Establish the connection to the power supply with the connecting cable.

• Before storing the centrifuge and the accessories it must be cleaned and if necessary disinfected and

decontaminated.

• Store the centrifuge in a clean, dust-free location.

• Be sure to place the centrifuge on its feet.

• Avoid direct sunlight.

2-4 Heraeus Pico 17 / 21 Thermo Scientific

3

Control Panel

Contents

• “Control Panel” on page 3-2

• “Keys” on page 3-2

Thermo Scientific Heraeus Pico 17 / 21 3-1

3 Control Panel

PULSE

OPEN

STOP

START

Run Time

Speed / RCF Value

Toggle k e y

Control Panel

Control Panel

In the control panel you find the keys and displays of the centrifuge. The main power switch is located
on the left side. All parameters can be selected and changed during operation.

Keys

The keys allow user intervention for controlling the operating mode as follows:

Key Display contents

Start

Stop

Open door

Pulse

Normal start of the centrifuge.

End run manually.

Automatic release (possible only when device is switched on); Emergency
release (see “Mechanical Emergency Door Release” on page 6-2).

By pressing the key the centrifuge starts immediately and
accelerates up to the end speed. Releasing the key initiates a stopping
process at the highest braking curves.

By pressing the key up and down you change the value in
thedisplay. If you hold the key pressed, the display changes continuously
at first slowly and after a few seconds at an accelerated pace to the
higher or lower values. By pressing the key briefly, you increase or
decrease the speed in one step. When pressing both arrows the cursor
moves to the left.

Changing the display
mode

3-2 Heraeus Pico 17 / 21 Thermo Scientific

Use the key to change the display mode. (speed / RCF value, sample /
chamber temperature, run time counter from start or preset speed on)

4

Operation

Contents

• “Switch on Centrifuge” on page 4-2

• “Lid Opening” on page 4-2

• “Close Door” on page 4-2

• “Rotor Installation” on page 4-2

• “Entering Parameters” on page 4-4

• “Centrifugation” on page 4-6

• “Short-term Centrifugation” on page 4-7

• “Removing the Rotor” on page 4-8

• “Aerosol-tight Rotors” on page 4-8

• “Audible Alarm” on page 4-8

• “Turning off the Centrifuge” on page 4-9

Thermo Scientific Heraeus Pico 17 / 21 4-1

4 Operation

Switch on Centrifuge

Switch on Centrifuge

1. Switch on the centrifuge.
The centrifuge shows the actual value in the display. Speed and run time show 0. The display
shows the current temperature of the sample.

Lid Opening

1. Press the key.
The display shows the following message:

Note Use the emergency release only for malfunctions and power failures (see “Mechanical

Emergency Door Release” on page 6-2).

Close Door

1. Close the door by pressing down on it lightly in the middle or on both sides of it.

Note The door should audibly click into place.

CAUTION Do not slam the door.

Rotor Installation

The approved rotors for the Heraeus Pico 17 / 21 are listed in section “Rotor Selection” on page 1-5.
Use only the rotors and accessories from this list in the centrifuge.

CAUTION Unapproved or incorrectly combined accessories can cause serious damage to
the centrifuge.

Proceed as follows:

1. Open the door of the centrifuge and if necessary remove any dust, foreign objects or residue from
the chamber.
Thread and o-ring must be clean and undamaged.

4-2 Heraeus Pico 17 / 21 Thermo Scientific

4 Operation

Rotor Installation

CAUTION Do not install the rotor when the temperature difference between shaft and
rotor lock is >20°C. Otherwise the rotor might jam during the installation. A jammed
rotor can lead to damages of centrifuge and rotor.

2. Hold the rotor over the centrifuge spindle. The two bars in the labeling on the upper side of the
rotor (a) must be above the retaining pin (b) of the centrifuge spindle.

a

b

3. Let the rotor slide slowly down the centrifuge spindle.

CAUTION Do not force the rotor onto the centrifuge spindle.
If the rotor is very light, then it may be necessary to press it onto the centrifuge spindle
with a bit of pressure.

4. Thread the fastening tool into the centrifuge spindle clockwise. Hold the rotor with the other hand
into position.

5. Close the rotor.

6. Check if the rotor is properly installed by lifting it slightly on the handle.

Thermo Scientific Heraeus Pico 17 / 21 4-3

4 Operation

Speed display

RCF display

Entering Parameters

WARNING Check for any damage to the rotor: Damaged rotors must not be used.
Keep the centrifuge spindle area of the rotor clear of objects.

CAUTION Check that the rotor is properly locked on the centrifuge spindle before each
use by pulling it at its handle. Fasten the rotor if necessary.

Be sure to check all sealings before starting any aerosol-tight applications.

See the information in the rotor instruction manual.

7. Close the centrifuge door.

Entering Parameters

Note Due to limited display digits there is a need to round the values. The direct comparison

between the two values speed and RCF is therefore restricted.

Select Speed or RCF-Value

1. Press the key under the left display. When the lower indicator is lit the display shows the speed.
Press the key to toggle between the two modes.

2. When the upper indicator is lit the display shows the RCF value. Press the key to toggle
between the two modes.

4-4 Heraeus Pico 17 / 21 Thermo Scientific

4 Operation

RCF 11 18,

n

1000

-------------



2

r××=

Entering Parameters

Pre-selecting Speed

1. Enter the desired value by pressing the key repeatedly, until the desired value shows.
You can adjust the speed in 100 rpm increments.

2. Press the key to confirm the pre-selected value.
If you do not press any key, the display flashes for a few seconds The new preselected value is now
stored and the display shows the actual value.

Note The centrifuge speed can be set to a minimum of 300rpm.

The maximum speed depends on the centrifuge variant.

Pre-selecting RCF-Value

1. Enter the desired value by pressing the key repeatedly, until the desired value shows.
You can adjust the RCF pre-selected value in steps of 100 xg.

2. Press the key to confirm the pre-selected value.
If you do not press any key, the display flashes for a few seconds The new preselected value is now
stored and the display shows the actual value.

Note The RCF value can be set to a minimum of 100 xg.

The maximum speed depends on the centrifuge variant.
The displayed RCF value is always corresponding to the maximum of centrifuge radius of the
micro liter rotor 24 x 2 (75003424).
Refer to the “Explanation of RCF-Value” on page 4-5 and the rotor manual for further
information.

Explanation of RCF-Value

The relative centrifugal force (RCF) is given as a multiple of the force of gravity g. It is a unitless
numerical value which is used to compare the separation or sedimentation capacity of various
centrifuges, since it is independent of the type of device. Only the centrifuging radius and the speed
come into play in it:

r = centrifuging radius in cm

n = rotational speed in rpm

The maximum RCF value is related to the maximum radius of the tube opening.

Thermo Scientific Heraeus Pico 17 / 21 4-5

Remember that this value is reduced depending on the tubes and adapters used.

This can be accounted for in the calculation above if required.

4 Operation

Centrifugation

Run Time Preselection

Note You can select a run time between 1 and 99 min or continuous operation.

1. Enter the desired value by pressing the key below the display in the middle repeatedly, until
the desired value shows.
You can adjust the run time in steps of 1 min.

2. Press the key to confirm the pre-selected value.
If you do not press any key, the display flashes for a few seconds The new preselected value is now
stored and the display shows the actual value.

Continuous Operation

1. Press the key until hd is shown.

2. During continuous operation, the centrifuge will continue running until you stop it manually with
the key.

CAUTION Please note that the lifetime of particularly plastic rotor tubes is
limited.Continuous operation (extended use) may cause damage to them.

Changing the settings during the run

You can change the settings during the run as follows:

1. Press one of the two
The current value will switch into the pre-selecting value mode.

keys in the control panel.

2. Enter the new value as described above.

3. Press the key.
The value is taken over immediately.

Centrifugation

Once the rotor has been properly installed, the main switch has been turned on and the door has been
closed, you can start centrifuging.

4-6 Heraeus Pico 17 / 21 Thermo Scientific

4 Operation

Short-term Centrifugation

Starting Centrifuge Program

Press the key on the control panel. The centrifuge accelerates to the preset speed with the time
display active.

The run display begins to count down from the pre-selected value. If the remaining run time is less
then 1 minute, the remaining time is given in seconds.

The circulating indicators in the left display represent the spinning rotor.

In continuous operation hd the time display counts up. The displayed run time is first in seconds. After
one minute the displays changes every minute.

Stopping the Centrifugation Program

With preset Run Time

If the run time has been pre-selected, and all you have to do, is to wait until the centrifuge terminates
the run automatically.

As soon as the speed drops to zero, the message END will appear in the display. By pressing the
key, you can open the door and remove the centrifuge material.

You can also stop the centrifuging program manually at any time by pressing the key.

Continuous Operation

If you selected continuous operation (see “Continuous operation” on page 4-6), you will have to stop
the centrifuge manually.

1. Press the key on the control panel.
The centrifuge will be decelerated at the designated rate.

2. Press the key to open the centrifuge door and the remove the samples when the message END
appears in the display.

Short-term Centrifugation

For short-term centrifuging, the Heraeus Pico 17 / 21 has a PULSE- function.

By holding down the key, spinning will start and continue until the key is let go.

The centrifuge accelerates and brakes at maximum power. The pre-selected value is ignored.

Note The centrifuge accelerates to the maximum speed.

Check carefully whether you have to maintain a certain speed for your application.

The displayed run time is first in seconds. After one minute the displays changes every minute.

After an short-term centrifugations the set values are restored.

Thermo Scientific Heraeus Pico 17 / 21 4-7

4 Operation

Removing the Rotor

Removing the Rotor

To remove the rotor, proceed as follows:

1. Open the centrifuge door.

2. Unscrew the rotor with the allen wrench.

3. Grab the rotor in the middle. Pull the rotor directly upwards and remove it from the centrifuge
spindle. Make sure not to tilt the rotor while doing this.

WARNING Be careful, when you change a rotor after a run. Centrifuge spindle and
motor bearing assembly can be hot (>55°C).

Aerosol-tight Rotors

When using an aerosol tight lid the rotor can be removed with the lid closed. This is to protect you and
the samples.

Audible Alarm

Error

Accompanying all error messages, a warning signal is given out.

Press any key to silence the warning signal.

End of Run

By default there is an acoustic signal at the end of any centrifugation run. To switch off this signal
proceed as follows:

Press the key when you turn on the centrifuge.
The display shows:

or

4. Press the key under the display in the middle.
The acoustic signal is turned on or off.

5. Press the key to confirm the pre-selected value.

4-8 Heraeus Pico 17 / 21 Thermo Scientific

Turning off the Centrifuge

1. To turn off the centrifuge put the mains switch to "0".

Note The centrifuge is equipped with a special switch for balancing potential voltage discrepancies

in the power grid. After pressing the mains switch the display therefore may still flash up to
10 seconds.

4 Operation

Turning off the Centrifuge

Thermo Scientific Heraeus Pico 17 / 21 4-9

5

Maintenance and Care

Contents

• “Cleaning Intervals” on page 5-2

• “Cleaning” on page 5-2

• “Disinfection” on page 5-3

• “Decontamination” on page 5-4

• “Autoclaving” on page 5-5

• “Service of Thermo Fisher Scientific” on page 5-5

Thermo Scientific Heraeus Pico 17 / 21 5-1

5 Maintenance and Care

Cleaning Intervals

Cleaning Intervals

For the sake of personal, environmental, and material protection, it is your duty to clean and if
necessary disinfect the centrifuge on a regular basis.

Maintenance Recommended Interval

Clean Rotor Chamber Daily or when polluted

Clean Rotor Daily or when polluted

Accessories Daily or when polluted

Cabinet Once per Month

Filter Unit Every three Months

Ventilation Holes Every six Months

CAUTION Refrain from using any other cleaning or decontamination procedure than
those recommended here, if you are not entirely sure that the intended procedure is safe
for the equipment.
Use only approved cleansers.
If in doubt, contact Thermo Fisher Scientific.

Cleaning

When cleaning centrifuge mind the following:

• Use warm water with a neutral solvent.

• Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions or

scrubbing powder.

• Rinse the cavities out thoroughly.

• Use a soft brush without metal bristles to remove stubborn residue.

• Afterwards rinse with distilled water.

• Place the rotors on a plastic grate with their cavities pointing down.

• If drying boxes are used, the temperature must never exceed 50°C, since higher temperatures could

damage the material and shorten the lifetime of the parts.

• Use only disinfectants with a pH of 6-8.

• Dry aluminum parts off with a soft cloth.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000

9824). Also treat the cavities with oil.

• Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing

down.

5-2 Heraeus Pico 17 / 21 Thermo Scientific

CAUTION Before using any cleaning or decontamination methods except those

recommended by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment.

Clean centrifuge and accessories as follows:

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Release the rotor.

5. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

6. Remove the centrifuge tubes and adapters.

7. Use a neutral cleaning agent with a pH value between 6 and 8 for cleaning.

5 Maintenance and Care

Disinfection

8. Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000

Disinfection

Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation.

maximum temperature of 50°C.

9824). Also treat the cavities with oil..

CAUTION When cleaning, do not allow liquids, especially organic solvents, to get on the
drive shaft or the bearings of the centrifuge.
Organic solvents break down the grease in the motor bearing. The drive shaft could freeze
up.

WARNING Infectious material can get into the centrifuge when a tube breaks or as a
result of spills. Keep in mind the risk of infection when touching the rotor and take all
necessary precautions.
In case of contamination, make sure that others are not put at risk.
Decontaminate the affected parts immediately.
Take other precautions if need be.

The rotor chamber and the rotor should be treated preferably with a neutral disinfectant.

CAUTION Before using any cleaning or decontamination methods except those
recommended by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment.
Observe the safety precautions and handling instructions for the cleaning agents used.

Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other
disinfectants.

Disinfect the rotor and accessories as follows:

Thermo Scientific Heraeus Pico 17 / 21 5-3

5 Maintenance and Care

Decontamination

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Release the rotor.

5. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

6. Remove the centrifuge tubes and adapters and dispose of them or disinfect them.

7. Treat the rotor and accessories according to the instructions for the disinfectant. Adhere strictly to

8. Be sure the disinfectant can drain off the rotor.

9. Rinse the rotor and accessories thoroughly with water.

10. Dispose of the disinfectant according to the applicable guidelines.

11. Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a

the given application times.

maximum temperature of 50°C.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000

9824). Also treat the cavities with oil.

Decontamination

Decontaminate the centrifuge immediately whenever radioactive material has spilled during
centrifugation.

WARNING Infectious material can get into the centrifuge when a tube breaks or as a
result of spills. Keep in mind the risk of infection when touching the rotor and take all
necessary precautions.
In case of contamination, make sure that others are not put at risk.
Decontaminate the affected parts immediately.
Take other precautions if need be.

CAUTION Before using any cleaning or decontamination methods except those
recommended by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment.

For general radioactive decontamination use a solution of equal parts of 70% ethanol, 10% SDS and
water.

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Release the rotor.

5. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

6. Remove the centrifuge tubes and adapters and dispose of them or disinfect them.

5-4 Heraeus Pico 17 / 21 Thermo Scientific

7. Rinse the rotor first with ethanol and then with de-ionized water.

• Adhere strictly to the given application times.

8. Be sure the decontamination solution can drain off the rotor.

9. Rinse the rotor and accessories thoroughly with water.

10. Dispose of the decontamination solution according to the applicable guidelines.

11. Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000

Autoclaving

1. Before autoclaving clean rotor and accessories as described above.

5 Maintenance and Care

Autoclaving

maximum temperature of 50°C.

9824). Also treat the cavities with oil.

2. Place the rotor on a flat surface.

• Rotors and adapter can be autoclaved at 121°C.

• The maximum permissible autoclave cycle is 20 minutes at 121°C.

Note No chemical additives are permitted in the steam.

CAUTION Never exceed the permitted temperature and duration when autoclaving.

If the rotor shows signs of corrosion or wear, it must be replaced.

Service of Thermo Fisher Scientific

Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an
authorized service technician. The service technician checks the following:

• the electrical equipment

• the suitability of the set-up site

• the door lock and the safety system

• the rotor

• the fixation of the rotor and the drive shaft

Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are
performed for free during the warranty period and afterwards for a charge.

This is only valid if the centrifuge has only been maintained by a Thermo Fisher Scientific service
technician.

Thermo Scientific Heraeus Pico 17 / 21 5-5

5 Maintenance and Care

Shipping and Deposing of Accessories

Shipping and Deposing of Accessories

WARNING Before shipping or deposing centrifuges and accessories you have ton

cleanand if necessary disinfect or decontaminate everything. Before storing the centrifuge
and the accessories it must be cleaned and if necessary disinfected and decontaminated.

5-6 Heraeus Pico 17 / 21 Thermo Scientific

6

Troubleshooting

Contents

• “Mechanical Emergency Door Release” on page 6-2

• “Troubleshooting by User” on page 6-3

• “When to contact a Service Technician” on page 6-6

Thermo Scientific Heraeus Pico 17 / 21 6-1

6 Troubleshooting

Emergency Door Release

Mechanical Emergency Door Release

Mechanical Emergency Door Release

During a power failure, you will not be able to open the centrifuge door with the regular electric lid
release. A mechanical override is provided to allow sample recovery in the case of an emergency.
However, this should be used only in emergencies and after the rotor has come to a complete stop.

WARNING The rotor can still be spinning at high speed. If touched, it can cause serious
injuries.

Always wait a few minutes until the rotor has come to a stop without braking. The brake does not work
when there is no current. The braking process lasts much longer than usual.

Proceed as follows:

1. Make sure the rotor has stopped (view port in the door).

WARNING Never use your hand or any tools to brake the rotor.

2. Pull out the power supply plug.

3. Insert a 3 inch long wire (e.g. a staple) into the hole above the control panel.

4. Press the centrifuge door down gently. Push the wire further into the hole until you hear and
feelthe door latch unlocking.

5. Remove the wire from the hole and open the centrifuge door.
Now you can remove your samples.

Figure 6-1.

Reconnect the centrifuge once the power has been restored. Switch on the centrifuge.

6-2 Heraeus Pico 17 / 21 Thermo Scientific

Emergency Door Release

Troubleshooting by User

If problems occur other than those listed in this table, the authorized customer service
representative must be contacted.

Failure message Problem with centrifuge Possible causes and cures

6 Troubleshooting

Troubleshooting by User

Display remains dark. The drive stops. The centrifuge decelerates

without braking. The centrifuge door cannot
be opened.

Display fails briefly The drive stops. The centrifuge decelerates

without braking.

The centrifuge door
cannot be opened.

Pressing key has no effect. Centrifuge door is not correctly engaged or warped.

Exceptionally running noise Imbalance

No mains connection.

1. Is the centrifuge turned on?

2. Check the mains connection.

If the display remains dark, inform a service technician.

Mains connection interrupted for some seconds.

1. Turn off mains switch.

2. Check whether the mains power cord is connected properly.

3. Restart the centrifuge.Restart the centrifuge.

1. Check if mains connection is working and the instrument is
switched on (display is lit).

2. If this is unsuccessful, you may open the centrifuge doorusing
the mechanical emergency door release (“Mechanical

Emergency Door Release” on page 6-2)

1. Stop the centrifuge. Press the key or unplug mains
powercord.

2. Wait until the centrifuge comes to a complete stop.

3. Check whether the rotor is properly loaded.

Check whether a broken tube, damage to the rotor or motor
isresponsible for the run noise.
If the running noise remains exceptionally, inform a service
technician.

Display oP appears
although lid is closed.

Lid Rotor stops with deceleration to standstill. Centrifuge door was opened manually during the run. Close

bAL Rotor stops with deceleration to standstill. Imbalance switch releases.

Thermo Scientific Heraeus Pico 17 / 21 6-3

Centrifuge does not start. Centrifuge door not properly closed. Open the centrifuge door

and repeat locking procedure.
If the message appears again, inform a service technician.

centrifuge door immediately.

Rotor stops with deceleration to standstill.

For further centrifugation, you have to switch the instrument off
and switch it on again

1. Open the centrifuge by pressing the key.

2. Check whether the rotor is properly loaded.

3. Check whether a broken tube or damaged rotor released the

imbalance switch.

If the message appears again, inform a service technician.

6 Troubleshooting

Troubleshooting by User

Failure message Problem with centrifuge Possible causes and cures

E-01 - E-13 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

E-14 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

E-15 - E-16 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

E-22 - E-23 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

E-24 The centrifuge cannot be operated. The

door cannot be opened.

Internal program error

Switch the instrument off and on again.

If the message appears again, inform a service technician.

Over-temperature in the centrifuge chamber.

Switch the centrifuge off and turn it on again after approx. one
minute.

If the message appears again, inform a service technician.

Temperature measurement error

Switch the instrument off and on again.

If the message appears again, inform a service technician.

Error in speed entry

Switch the instrument off and on again.

The display shows BR and a countdown from 100 - 0.

If the message appears again, inform a service technician.

Wrong status information from the door latch.

1. Switch the instrument off and on again.

2. After re-switching on, the display shows Lid FAiL.

3. If the centrifuge door has been already opened, the
displayshows CLOSE Lid. Thereupon close the door.

4. The centrifuge tries to open the door to switch for starting
thenormal operation mode.

If the message appears again, inform a service technician.

E-29 Motor does not start. Motor or rotor blocked

1. Switch the instrument off and on again using the mains
switch.

2. Door Opening

3. Check whether the rotor can turn freely.

If the message appears again, inform a service technician.

E-31 Rotor stops without deceleration to

standstill or does not start.

Overtemperature in the motor.

1. Turn instrument off and unplug mains power cord.

2. Check and clean the venting slots if necessary and
respectively the filter unit of the cooled centrifuge.

3. After approx. 60 minutes you can restart the instrument.

Observe the maximum permissible environmental temperature.

If the message appears again, inform a service technician.

6-4 Heraeus Pico 17 / 21 Thermo Scientific

Failure message Problem with centrifuge Possible causes and cures

E-33 Rotor stops with deceleration to standstill. Overpressure in the refrigeration unit

1. Wait 15 minutes before switching off the centrifuge.
The fan will operate at maximum power in order to reduce the
overpressure.

2. Check the draft at the back of the centrifuge if you cannot
here the fan.

If you do not feel any draft:

1. Turn instrument off and unplug mains power cord.

2. Check and clean the venting slots if necessary and
respectively the filter unit of the cooled centrifuge.

3. After approx. 30 minutes you can restart the instrument.

Observe the maximum permissible environmental temperature.

If the message appears again, inform a service technician.

6 Troubleshooting

Troubleshooting by User

E-36 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

Over-current or over-voltage, the brake resistance the hot.

After approx. 60 minutes you can restart the instrument.

Switch the instrument off and on again.

If the message appears again, inform a service technician.

E-39 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

Error in speed entry.

Switch the instrument off and on again.

If the message appears again, inform a service technician.

E-41 - E-56 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

Internal program error

Switch the instrument off and on again.

If the message appears again, inform a service technician.

E-60 Rotor stops with deceleration to standstill. Under-temperature in the centrifuge chamber.

1. Stop the centrifugation run.

2. Open the centrifuge door and defrost the chamber.
Never touch the chamber directly with your hands – you
mayfreeze up.

3. After approx. 60 minutes you can restart the instrument.

Observe the maximum permissible ambient temperature.

4. If a strong ice sheet is present in the internal chamber, besure
to remove all condensate after defrosting.

If the message appears again, inform a service technician.

E-63 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

Temperature measurement error

Switch the instrument off and on again.

If the message appears again, inform a service technician.

E-64 The cooling system could not be started. Power supply undervoltage

Check the power supply and contact you supplier if necessary.
The message LINE appears in the display.

E-65 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

Temperature measurement error

Switch the instrument off and on again.

If the message appears again, inform a service technician.

Thermo Scientific Heraeus Pico 17 / 21 6-5

6 Troubleshooting

When to contact a Service Technician

When to contact a Service Technician

If you need to contact a service technician, please provide the order no. and the serial no. of your
centrifuge. This information can be found on the back near the inlet for the power supply cable.

To identify the software version, proceed as follows:

1. Hold down the key and then switch on the centrifuge.
In the display all segments are lit.

• Subsequently, the following readings will be displayed for 5 seconds each:

Software number SOFT 058 3_

Software version _01

NV-RAM number EEPRO 462 1_

NV-RAM version _01

Cycle counter CYCLE 001 25

• The values shown above are just examples.

Software 0583 Version 01

NV-RAM 4521 Version 01

cycles completed 125

2. Communicate the software version to the service technician.

6-6 Heraeus Pico 17 / 21 Thermo Scientific

A

Chemical Compatibility Chart

CHEMICAL

2-mercaptoethanol

Acetaldehyde

Acetone

Acetonitrile

Alconox

Allyl Alcohol

Aluminum Chloride

Formic Acid (100 %)

Ammonium Acetate

Ammonium Carbonate

Ammonium Hydroxide (10 %)

Ammonium Hydroxide (28 %)

Ammonium Hydroxide (conc.)

Ammonium Phosphate

Ammonium Sulfate

Amyl Alcohol

Aniline

Sodium Hydroxide (<1 %)

Sodium Hydroxide (10 %)

Barium Salts

Benzene

Benzyl Alcohol

Boric Acid

Cesium Acetate

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

SSU- SMS- SUSSUSS- SSSSUSSSSSS

S-UU---M-U---MUUUMM- MSU- S- U

MSUUSUMSSUUSUSUUUSSUUSMMSUU

SSU- SMS- SSUSUMUU- SMUUSSSSUU

UUS- SSS- SSSSSSMSSSSSSSSSSSU

---U--S----S-SSMSSS-MS--S--

UUSSSSUSSSSMSSSS- SSSSSMUUSS

-SMU--U----U-SMUUSS-US-US-U

SSU- SSS- SSSSSSSU- SSSSSSSSSS

MSUSSSSSSSSSSSUU- SSSSSSMSSS

UUSUSSMSSSSS- SUMSSSSSSSSSMS

UUSUSUMSSSSSUSUMSSSSSSSSSMS

UUUUSUMS- S- SUSUUSSS- MSSSS- U

U- S- SSSSSSSS- SSM- SSSSSSMSSS

UMS- SSUSSSSSSSSS- SSSSSSUSSU

S- MU- - SS- M- S- MSSSSM---U-S-M

SSUUSUSMSUUUUUUU- SMUUSSSSUS

U- MSSS- - SMSS- SMMSSSSSSMSS- U

U- MU- - U- MMSSUSUUSSSSSSMSS- U

MUS- SSSSSSSSSSSM- SSSSSSMSSS

SSUUSUMUSUUSUUUMUMUUUSUUS US

S- UU- - MM- M- SUUUUUUU- MSM- S- S

USSMSSUSSSSSSSSSUSSSSSSSSSS

M- S- SSS- SSSS- SS- - SSSSSSMSSS

, POLYCLEAR,CLEARCRIMP

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

VITON

Thermo Scientific Heraeus Pico 17 / 21 A-1

A Chemical Compatibility Chart

CHEMICAL

Cesium Bromide

Cesium Chloride

Cesium Formate

Cesium Iodide

Cesium Sulfate

Chloroform

Chromic Acid (10 %)

MATERIAL

, POLYCLEAR,CLEARCRIMP

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

VITON

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSSUSSS- SSSSSSS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

UUUUSSMUSUUMUMUUUMMUUSUUUMS

U- UUSUU- SSSUSSMUMSSUMSMUSSS

Chromic Acid (50 %)

Cresol Mixture

Cyclohexane

Deoxycholate

Distilled Water

Dextran

Diethyl Ether

Diethyl Ketone

Diethylpyrocarbonate

Dimethylsulfoxide

Dioxane

Ferric Chloride

Acetic Acid (Glacial)

Acetic Acid (5%)

Acetic Acid (60 %)

Ethyl Acetate

Ethyl Alcohol (50 %)

Ethyl Alcohol (95 %)

Ethylene Dichloride

Ethylene Glycol

Ethylene Oxide Vapor

Ficoll-Hypaque

Hydrofluoric Acid (10 %)

Hydrofluoric Acid (50 %)

Hydrochloric Acid (conc.)

U-UU-UU---SUUSMUMSSUMS-UM-S

SSU---S-SUUUUUU- - UU- USSSSUS

SSS- SSSUSUSSUUUMSMUMMSUMMUS

SSS- SSS- SSSSSSS- - SSSSSSSSSS

SSSSSSSSSSSSSSSSSSSSSSSSSSS

MSSSSSS- SSSSSSSSSSSSSSSMSSS

SSUUSSSUSUUSUUUUUUUUUSSSSMU

S- UU- - M- SU- S- MUUUMM- US- - SUU

SSU- SSS- SSUSUSU- - SSSMSSSSSS

SSUUSSS- SUSSUSUU- SSUUSSSSUU

MSUUSSMMSUUSUMUU- MMMUSSSSUU

UUS---MS-M-S-S---SS---MUS-S

SSUUSSUMSUSUUUUUMSUMUSUUS- U

SSMSSSMSSSSSMSSSSSSSMSSMSSM

S S U U S S U - S MS U U MU S MS MS MS MU S MU

MMUUSSMMSS USUMUU- SS UUSMMSUU

SSSSSSMSSSSSUSUSSSSSSSSMSMU

SSSUSSMSSSSSUSU- SSSMSSSUSMU

S- UU- - SM- UUSUUUUUUU- USU- S- S

SSSSSSSSSSSS- SUSSSSSSSSMSMS

S- U- - U- - SU- S- SM- - SSSUSUSSSU

MSS- SSS- SSSS- SS- SSSSSSSMSSS

UUUM- - U- - UUS- SMUSSSSMSUUU- -

UUUU- - U- - UUUUSUUUSS MMSUUU- M

UUUU- UUM- UMUUMUUU- S- USUUU- -

A-2 Heraeus Pico 17 / 21 Thermo Scientific

CHEMICAL

Formaldehyde (40 %)

Glutaraldehyde

Glycerol

Guanidine Hydrochloride

Haemo-Sol

Hexane

A Chemical Compatibility Chart

, POLYCLEAR,CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

MMMSSSSMSSSSMSSSUSSMSSSMSMU

SSSS- - S- SSSSSSS- - SSS- - SSS- -

MSS- SSSSSSSSSSSS- SSSSSSSSSS

UUS- SSS- SSSSSSS- - SSSSSSUSSS

SSS---S-SSSSSSS- - SSSSSSSSSS

SSS- SSS- SSUSUMUSSUSSMSUSSUS

VITON

Isobutyl Alcohol

Isopropyl Alcohol

Iodoacetic Acid

Potassium Bromide

Potassium Carbonate

Potassium Chloride

Potassium Hydroxide (5 %)

Potassium Hydroxide (conc.)

Potassium Permanganate

Calcium Chloride

Calcium Hypochlorite

Kerosene

Sodium Chloride (10 %)

Sodium Chloride (sat'd)

Carbon Tetrachloride

Aqua Regia

Solution 555 (20 %)

Magnesium Chloride

Mercaptoacetic Acid

Methyl Alcohol

Methylene Chloride

Methyl Ethyl Ketone

Metrizamide

Lactic Acid (100 %)

Lactic Acid (20 %)

N-Butyl Alcohol

- - MU- - SS- U- SUSSMSSS- SSS- S- S

MMMU SSSSSUSSUSUMSSSSSSSMMMS

SSM- SSS- SMSSMSS- MSSSSSMSSMM

USS- SSS- SSSSSSSSSSS- SSSMSSS

MUSSSSS- SSSSSSUSSSSSSSSSSSS

USS- SSSSSSSSSSS- SSSSSSSUSSS

UUSSSSM- SSSS- SUSSSSSSSMUMSU

UUMU- - M- MSS- UMUUUSM- MU- UU- U

SSS- SSS- SSSUSSSM- SMSUSSMSUS

MUSSSSSSSSSSSSMS- SSSSSSMSSS

M- U- SMMS- M- S- SMS- SSSMSMUS- S

SSS- SSSUSMUSUMMS- MMMSSUSSUS

S- SSSSSS- - - SSSSS- SSSS- SSM- S

U-SUSSS----SSSSS- SS- S- SSM- S

U U MS S U MU S U U S U MU S S MMS MMMMU S S

U-UU--U-----UUUUUUU-----S-M

SSS---S-SSSSSSS- - SSS- SSSSSS

MSS- SSSSSSSSSSSSSSSSSSSMSSS

USU- SMS- SMSUUUU- SUUSMSUSSSS

SSSUSSMSSSSSUSUMSSSSSSSMSMU

UUUUMSSUSUUSUUUUUMUUUSSMUSU

SSUUSSMSSUUSUSUUUSSUUSSSSUU

MSS- SSS- SSSS- SS- - SSSSSSMSSS

--S------MSU-SSSMSS- MSMSS- S

--SS-----MSM-SSSSSSSMSMSS- S

S- SU- - S- - SM- USMSSSSMMSM- S- S

Thermo Scientific Heraeus Pico 17 / 21 A-3

A Chemical Compatibility Chart

CHEMICAL

N-Butyl Phthalate

N, N-Dimethylformamide

Sodium Borate

Sodium Bromide

Sodium Carbonate (2 %)

Sodium Dodecyl Sulfate

Sodium Hypochlorite (5 %)

Sodium Iodide

Sodium Nitrate

Sodium Sulfate

Sodium Sulfide

Sodium Sulfite

Nickel Salts

Oils (Petroleum)

Oils (Other)

Oleic Acid

Oxalic Acid

Perchloric Acid (10 %)

Perchloric Acid (70 %)

Phenol (5 %)

Phenol (50 %)

Phosphoric Acid (10%)

Phosphoric Acid (conc.)

Physiologic Media (Serum, Urine)

Picric Acid

Pyridine (50 %)

Rubidium Bromide

MATERIAL

, POLYCLEAR,CLEARCRIMP

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

VITON

SSU- SSS- SUUSUUUM- UUSUSMMSUS

SSSUSMS- SSUSUSUU- SSUUSMSSSU

MSSSSSSSSSSUSSSS- SSSSSSMSSS

USS- SSS- SSSSSSSS- SSSSSSMSSS

MUSSSSSSSSSSSSUSSSSSSSSSSSS

SSS- SSS- SSSSSSS- SSSSSSSSSSS

UUMSSMUSSMSSSMSSSSMSSSMUSMS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

SSS- SSSSSSSSSSSS- SSSSSUSSSS

USS- SSSSSSSSSSSSSSSSSSSMSSS

S-SS---S---SSSUU- - S- - - SSM- S

SSS- SSSSMSSSSSSM- SSSSSSSSSS

USSSSS- SSS- - SSSS- SSSSSSMSSS

SSS---SUSSSSUUMSMUUSSSUSSSS

S-S---SMSSSSUSSSSUSSSS- SSMS

S- USSSUUSUSSMSSSSSSSSSMUSMM

UUMSSSUSSSSSUSUSSSSSSSSUMSS

U- U- SUU- SMM- - MUMSMM- MSU- S- S

UUU- - UU- SUMUUMUUUMMUMSUUSUS

U S U - S MM- S U MU U S U MS MS U U S U MMMS

USU- SUM- SUMUUUUUSUMUUSUUUMS

UUMSSSUSSSSU- SSSSSSSSSUMUSS

U U MM- - U S - MS U U MMS S S MS MS U MU - S

MSSS- - S- SSSSSSSSSSSSSSSSSSS

SSU- SMSSSMSUSSSUSSSSUSUMSMS

USUUSUU- USSUUMUU- USMUSSUUUU

MSS- SSS- SSSSSSS- - SSSSSSMSSS

Rubidium Chloride

Sucrose

Sucrose, Alkaline

Sulfosalicylic Acid

Nitric Acid (10 %)

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSS- SSSSSSSSSSSSSSSSSSSSSSS

MSS- SSS- SSSSSSUSSSSSSSSMSSS

UUSSSSS- SSSUSSS- SSS- SSSUSSS

USUSSUU- SUSU- SSSSSSSSSMSSSS

A-4 Heraeus Pico 17 / 21 Thermo Scientific

CHEMICAL

Nitric Acid (50 %)

Nitric Acid (95 %)

Hydrochloric Acid (10 %)

Hydrochloric Acid (50 %)

Sulfuric Acid (10 %)

Sulfuric Acid (50 %)

Sulfuric Acid (conc.)

Stearic Acid

Tetrahydrofuran

Toluene

Trichloroacetic Acid

Trichloroethane

Trichloroethylene

Trisodium Phosphate

Tris Buffer (neutral pH)

Triton X-100

Urea

Hydrogen Peroxide (10 %)

Hydrogen Peroxide (3 %)

Xylene

Zinc Chloride

Zinc Sulfate

Citric Acid (10 %)

1

Polyethyleneterephthalate

A Chemical Compatibility Chart

, POLYCLEAR,CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

USUMSUU- SUSUUMMUMMMSSSUSSMS

U- UU- UU- - UUUUMUUUUMUUSUSS- S

UUMSSSU- SSSUUSUSSSSSSSSUMSS

UUUUSUU- SMSUUMUUSSSSMSMUUMM

MUUSSUU- SSMUSSSSSSSSSSUUUSS

MUUUSUU- SSMUUSUUMSSSSSUUUMS

MUUU- UUM- - MUUSUUUMSUMSUUU- S

S-S---SMSSSS- SSSSSSSSSMMSSS

SSUUSUUMSUUSUUU- MUUUUSUSSUU

SSUUSSMUSUUSUUUSUMUUUSUSUUM

UUU- SSUMSUSUUSM- MSSUUS UUUMU

S-U---MU-U-SUUUUUUUUUSU- S- S

--UU---U-U-SUUUUUUUUUSU- U- S

---S--M------S--SSS--S--S-S

USSSSSS- SSSSSSSSSSSSSSSSSSS

SSS- SSS- SSSSSSSSSSSSSSSSSSS

S- USSSS----SSSMSSSS- SSSMS- S

UUMSSUU- SSSUSSSMUSSSSSSMSUS

SMSSS- S- SSSSSSSSMSSSSSSSSSS

SSUSSSMUSUUUUUUMUMUUUSUMSUS

UUSSSSUSSSSSSSSSSSSSSSSUSSS

USS- SSSSSSSSSSSSSSSSSSSSSSS

MSSMSSMSSSSSSSSSMSSSSSSSSSS

VITON

Thermo Scientific Heraeus Pico 17 / 21 A-5

A Chemical Compatibility Chart

Key

S Satisfactory

M Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.

Suggest testing under actual conditions of use.

U Unsatisfactory, not recommended.

-- Performance unknown; suggest testing, using sample to avoid loss of valuable material.

Chemical resistance data is included only as a guide to product use. No organized chemical resistance
data exists for materials under the stress of centrifugation. When in doubt we recommend pretesting
sample lots.

A-6 Heraeus Pico 17 / 21 Thermo Scientific

B

Contact Information

United States / Canada 866-9-THERMO

Austria [phone]

Belgium [phone]

Germany 08001 536 376

France [phone]

Italy [phone]

Netherlands [phone]

Nordic / Baltic Countries / CIS [phone]

Russia [phone]

Spain / Portugal [phone]

Switzerland [phone]

[phone]

[phone]

UK / Ireland [phone]

China [phone]

[phone]

India 1800 22 8374

[phone]

Japan [phone]

Other Asian Countries [phone]

Australia [phone]

New Zealand [phone]

Other Countries [phone]

Thermo Scientific Heraeus Pico 17 / 21 B-1

S

Index

A

Accident Prevention .......................................... iv

Aerosol-tight Rotors ........................................ 4-8

Audible Alarm .................................................4-8

Autoclaving ..................................................... 5-5

B

Before Setting up .............................................2-2

Before Use ....................................................... 2-1

C

Care ................................................................ 5-1

Centrifugation ................................................. 4-6

Characteristics ................................................. 1-2

Chemical Compatibility Chart ....................... A-1

Cleaning ..........................................................5-2

Cleaning Intervals ........................................... 5-2

Close Door ...................................................... 4-2

Contact Information .......................................B-1

Continuous Operation ....................................4-6

Control Panel ..................................................3-2

D

Decontamination ............................................ 5-4

Deposing ......................................................... 5-6

Directives ........................................................1-4

Disinfection .................................................... 5-3

G

Guidelines .......................................................1-4

I

Intended Use ..................................................... iv

K

Keys ................................................................ 3-2

L

Leveling ........................................................... 2-3

Lid Opening ....................................................4-2

Location ..........................................................2-2

M

Mains Connection ...........................................2-3

Mains Supply .................................................. 1-5

Maintenance ...................................................5-1

O

Operation ........................................................ 4-1

P

Precautions ......................................................... v

Preface ............................................................... iii

E

Emergency Door Release .................................6-2

End of Run - Alarm ........................................ 4-8

Entering Parameters ........................................ 4-4

Error - Alarm ..................................................4-8

Explanation of RCF-Value ..............................4-5

RCF-Value ......................................................4-4

Removing the Rotor ........................................4-8

Rotor Installation ............................................ 4-2

Rotor Selection ................................................1-5

S

R

F

Features ........................................................... 1-4

Functions ........................................................1-4

Thermo Scientific Heraeus Pico 17 / 21 S-1

Scope of Supply ................................................. iii

Service .............................................................5-5

Service Technician ..........................................6-6

Index:

Shipping ..........................................................5-6

Short-term Centrifugation ..............................4-7

Speed .............................................................. 4-4

Standards ........................................................ 1-4

Storage ............................................................ 2-4

Switch on ........................................................4-2

T

Technical Data ................................................ 1-2

Transporting ................................................... 2-2

Troubleshooting .......................................6-1, 6-3

Turning off .....................................................4-9

S-2 Heraeus Pico 17 / 21 Thermo Scientific