Thermo Heraeus Fresco 17, Heraeus Fresco 21 User manual

Thermo Scientific

Heraeus Fresco 17 / 21

Instruction Manual

50133376-2 August 2012

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ThermoFisher

SCIENTIFIC

KON FORM ITATSE RKLARU

DECLARATION

Name

für die Zusammenstellung der relevanten technischen

Name

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Thermo Electron LED GmbH
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brand

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from equipment no.

mit

allen einschlägigen Bestimmungen der

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conformity with all relevant terms of directive

nachstehend beschriebene

machinery

described

Richtlinie über ln-vitro-Diagnostika

below

: Labor-Zentrifuqe / centrifuqe
: Heraeus
: FRESCO 17, FRESCO 21
: 75002420 75002423 75002425

: 41385539

for in vitro diagnostic medical

Unterlagen:

Maschine

(€

98/79/EG

devices 98n9/EC.

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Die Maschine ist
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Die Schutzziele nach Anhang 1 der Maschinenrichtlinie

2006/9 s/EG we rden ei nge h alte n.

protection goals

The

met.

Angewandte harmonisierte Normen/

Harmonized standards used:

EN 61010-1:2004
EN 61010-2-020: 2006

EN 61010-2-101:2002
EN 61326-1: 2006
EN 61326-2-6: 2006
EN 5501 18:2007

Osterode. den O9.O7 .2012

,'+r--d.aqrz

Andreas Karl,

Dr.

Director R&D

Datum, Name, Funktion und Unterschrift

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of directives for

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und der Niederspannungsrichtlinie

low voltage 2006/95/EC

and

are

WEEE Conformity

This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment
(WEEE) Directive 2002/96. It is marked by the following symbol:

Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU
Member States for the recycling and disposal of this device.

T

Table of Contents

Preface .......................................................................................................................................iii

Scope of Supply ....................................................................................................................... iii

Intended Use ..............................................................................................................................iv

Accident Prevention .................................................................................................................iv

Precautions .................................................................................................................................v

Chapter 1 Introduction and Description ...............................................................................................1-1

Characteristics of the Heraeus Fresco 17 / 21 ........................................................... 1-2

Technical Data ........................................................................................................ 1-3

Directives, Standards and Guidelines ...................................................................................... 1-4

Functions and Features ............................................................................................................ 1-5

Mains Supply ........................................................................................................... 1-5

Rotor Selection ........................................................................................................ 1-6

Chapter 2 Before Use ...............................................................................................................................2-1

Before Setting up ..................................................................................................... 2-2

Transporting the Centrifuge .................................................................................... 2-2

Location .................................................................................................................. 2-2

Aligning the Centrifuge ........................................................................................... 2-3

Mains Connection ................................................................................................... 2-3

Storage ..................................................................................................................... 2-4

Chapter 3 Control Panel ..........................................................................................................................3-1

Control Panel .......................................................................................................... 3-2

Keys ......................................................................................................................... 3-2

Chapter 4 Operation .................................................................................................................................4-1

Switch on Centrifuge ............................................................................................... 4-2

Door Opening ......................................................................................................... 4-2

Close Door .............................................................................................................. 4-2

Rotor Installation ..................................................................................................... 4-2

Entering Parameters ................................................................................................. 4-4

Centrifugation ......................................................................................................... 4-8

Short-term Centrifugation ....................................................................................... 4-8

Removing the Rotor ................................................................................................ 4-9

Aerosol-tight Rotors ................................................................................................. 4-9

Audible Alarm ......................................................................................................... 4-9

Turning off the Centrifuge .................................................................................... 4-10

Chapter 5 Maintenance and Care .........................................................................................................5-1

Cleaning Intervals .................................................................................................... 5-2

Cleaning .................................................................................................................. 5-2

Disinfection ............................................................................................................. 5-4

Thermo Scientific Heraeus Fresco 17 / 21 i

Contents

Decontamination ..................................................................................................... 5-5

Autoclaving .............................................................................................................. 5-5

Service of Thermo Fisher Scientific .......................................................................... 5-6

Shipping and Deposing of Accessories ..................................................................... 5-6

Chapter 6 Troubleshooting ....................................................................................................................6-1

Mechanical Emergency Door Release ...................................................................... 6-2

Troubleshooting by User ......................................................................................... 6-3

When to contact Service Technician ........................................................................ 6-5

Chemical Compatibility Chart ...............................................................................A-1

Contact Information ................................................................................................B-1

ii Heraeus Fresco 17 / 21 Thermo Scientific

P

Preface

Before starting to use the centrifuge, read through this instruction manual carefully and follow the
instructions.

The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.

Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty.

Scope of Supply

Article number Quantity Check

Centrifuge Heraeus Fresco 17 / 21 1

Power Supply Cable 1

Rotor 1

20360104 Allen Wrench 1

50133376 CD with Instruction Manual 1

If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.

This symbol refers to general hazards.
WARNING means that injuries or material damage or contamination could occur.
CAUTION means that material damage could occur.

This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

This symbol refers to hot surfaces
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

Thermo Scientific Heraeus Fresco 17 / 21 iii

P

g

cm

3

---------- -

Remaining risk: Inproper use can cause damages, contamincation, and inju­ries with fatal consequences.

Intended Use

Intended Use

• This centrifuge is a laboratory product designed to separate components by generation of Relative

Centrifugal Force. It separates human samples (e.g. blood, urine and other body fluids) collected in
appropriate containers, either alone or after addition of reagents or other additives.

• As general purpose centrifuge, it is designed to also run other containers filled with chemicals,

environmental samples and other non-human body samples.

• Together with the hematocrit rotor 75003473 the centrifuge can be used as an in-vitro diagnostics

device to determine the haematicrit value by using centrifugation. Apply DIN 58933 to determine
the hematocit value.

• The centrifuge is to be used for separating materials of different density or particle size suspended

in a liquid.

Maximum sample density at maximum speed: 1.2

Accident Prevention

WARNING If a hazardious situation occurs, turn off the power supply to the centrifuge

and leave the area immediately.

WARNING In case of a rotor failure the centrifuge can be damanged. The cooling agent
can escape. Ventilate the room well and leave it. Inform Customer Service.

Prerequisite for the safe operation of the Heraeus Fresco 17 / 21 is a work environment in
compliance with standards, directives and trade association safety regulations and proper
instruction of the user.

The safety regulations contain the following basic recommendations:

• Maintain a radius of at least 30 cm around the centrifuge.

• Implementation of special measures which ensure that no one can approach the

centrifuge for longer than absolutely necessary while it is running.

• The mains plug must be freely accessible at all times. Pull out the power supply plug or

disconnect the power supply in an emergency.

iv Heraeus Fresco 17 / 21 Thermo Scientific

Precautions

P

Precautions

In order to ensure safe operation of the Heraeus Fresco 17 / 21, the following general safety
regulations must be followed:

• The centrifuge should be operated by trained specialists only.

• The centrifuge is to be used for its intended use only.

• While handling centrifuge, rotor, and samples you must wear laboratory clothing (e.g.

gloves).

• Plug the centrifuge only into sockets which have been properly grounded.

• Do not move the centrifuge while it is running.

• Do not lean on the centrifuge.

• Use only rotors and accessories for this centrifuge which have been approved by Thermo

Fisher Scientific. Exceptions to this rule are commercially available glass or plastic
centrifuge tubes, provided they have been approved for the speed or the RCF value of
the rotor.

• Do not use rotors which show any signs of corrosion and/or cracks.

• Do not touch the mechanical components of the rotor and do not make any changes to

the mechanical components.

• Use only with rotors which have been properly installed. Follow the instructions in

section “Rotor Installation” on page 4-2.

• Use only with rotors which have been loaded properly. Follow the instructions given in

the rotor manual.

• Never overload the rotor. Follow the instructions given in the rotor manual.

• Never open the lid until the rotor has come to a complete stop and this has been

confirmed in the display.

• The lid emergency release may be used in emergencies only to recover the samples from

the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door

Release” on page 6-2).

• Never use the centrifuge if parts of its cover panels are damaged or missing.

• Do not touch the electronic components of the centrifuge or alter any electronic or

mechanical components.

• Please observe the safety instructions.

Thermo Scientific Heraeus Fresco 17 / 21 v

P

Remaining risk: Inproper use can cause damages, contamincation, and inju­ries with fatal consequences.

Remaining risk: Inproper use can cause damages, contamincation, and inju­ries with fatal consequences.

Precautions

Please pay particular attention to the following aspects:

• Location: well-ventilated environment, set-up on a level and rigid surface with adequate

load-bearing capacity.

• Rotor installation:Check that the rotor is locked properly into place before operating the

centrifuge.

• Especially when working with corrosive samples (salt solutions, acids, bases), the

accessory parts and rotor chamber have to be cleaned carefully.

• Always balance the samples.

Centrifuging hazardous substances:

• Do not centrifuge explosive or flammable materials or substances which could react

violently with one another.

• The centrifuge is neither inert nor protected against explosion. Never use the centrifuge

in an explosion-prone environment.

• Do not centrifuge inflammable substances.

• Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms

without suitable safety precautions.

When centrifuging microbiological samples from the Risk Group II (according to the
Biosafety Manual" of the World Health Organization (WHO)), aerosol-tight biological
seals have to be used.

For materials in a higher risk group, extra safety measures have to be taken.

• If toxins or pathogenic substances have gotten into the centrifuge or its parts,

appropriate disinfection measures have to be taken (see “Disinfection” on page 5-4).

• Highly corrosive substances which can cause material damage and impair the

mechanical stability of the rotor, should only be centrifuged in corresponding protective
tubes.

vi Heraeus Fresco 17 / 21 Thermo Scientific

1

Introduction and Description

Contents

• “Characteristics of the Heraeus Fresco 17 / 21” on page 1-2

• “Technical Data” on page 1-3

• “Directives, Standards and Guidelines” on page 1-4

• “Functions and Features” on page 1-5

• “Mains Supply” on page 1-5

• “Rotor Selection” on page 1-6

Thermo Scientific Heraeus Fresco 17 / 21 1-1

1

Introduction and Description

Characteristics of the Heraeus Fresco 17 / 21

Characteristics of the Heraeus Fresco 17 / 21

You can use various rotors with commercially available containers.

The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and
low-vibration operation even at high speeds, and guarantees a very long lifetime.

The user-friendly control panel makes it easy to pre-set the speed, RCF value, running time, and
temperature. You can choose between the display of speed and RCF or the entry mode.

These settings can be changed even while the centrifuge is running.

With the help of the key you can also centrifuge a sample for just a few seconds, if called for.

The Heraeus Fresco 17 / 21 is equipped with various safety features:

• The housing is made of high-impact resistant plastic and the interior of armour steel.

• The lid is equipped with a view port and a lock.

• The lid of the centrifuge can only be opened while the centrifuge is switched on and the rotor has

come to a complete stop. The centrifuge cannot be started until the lid has been closed properly.

• An electronic imbalance recognition in ventilated centrifuges.

• For emergencies only, e.g. during power failures (see “Mechanical Emergency Door Release” on

page 6-2)

1-2 Heraeus Fresco 17 / 21 Thermo Scientific

Technical Data

The technical data of the Heraeus Fresco 17 / 21 is listed in the following table.

Table 1- 1. Technical Data Heraeus Fresco 17

Feature Value

Environmental Conditions -Use in interior Spaces

1

Introduction and Description

Technical Data

-Altitudes of up to 2,000 m above Sea Level

-Max. relative Humidity 80% up to 31°C; Decreasing linearly up to
50% relative Humidity at 40°C

Permissible Ambient Temperature during

+5°C bis +40°C +5°C bis +40°C +5°C bis +40°C

Operation

Permissible Ambient Temperature during

+2°C bis +50°C +2°C bis +50°C +2°C bis +50°C

Storage and Shipping

Overvoltage Category II II II

Pollution Degree 2 2 2

Heat Dissipation Heraeus Fresco 17 Heraeus Fresco 17 Heraeus Fresco 17

230 V 120 V 100 V

614 BTU/h 614 BTU/h 580 BTU/h

IP 20 20 20

Run Time unlimited unlimited unlimited

Max Speed n

Min Speed n

max

min

Maximum RCF Value at n

max

13 300 rpm
(Depending on
Rotor)

13 300 rpm
(Depending on
Rotor)

13 300 rpm
(Depending on
Rotor)

300 rpm 300 rpm 300 rpm

17 000 x g 17 000 x g 17 000 x g

Maximum Kinetic Energy <1,90 kNm <1,90 kNm <1,90 kNm

Noise Level at maximum Speed < 56 dB (A) < 56 dB (A) < 56 dB (A)

Dimensions

Hight
Width
Depth

230 mm
240 mm
350 mm

230 mm
240 mm
350 mm

230 mm
240 mm
350 mm

Weight without Rotor 10.5 kg 10.5 kg 10.5 kg

Table 1- 2. Technical Data Heraeus Fresco 21

Feature Value

Environmental Conditions -Use in interior Spaces

-Altitudes of up to 2,000 m above Sea Level

-Max. relative Humidity 80% up to 31°C; Decreasing linearly up to
50% relative Humidity at 40°C

Permissible Ambient Temperature during
Operation

Permissible Ambient Temperature during
Storage and Shipping

Thermo Scientific Heraeus Fresco 17 / 21 1-3

+5°C bis +40°C +5°C bis +40°C +5°C bis +40°C

+2°C bis +50°C +2°C bis +50°C +2°C bis +50°C

1

Introduction and Description

Directives, Standards and Guidelines

Table 1-2. Technical Data Heraeus Fresco 21

Feature Value

Overvoltage Category II II II

Pollution Degree 2 2 2

Heat Dissipation Heraeus Fresco 21 Heraeus Fresco 21 Heraeus Fresco 21

IP 20 20 20

Run Time unlimited unlimited unlimited

230 V 120 V 100 V

785 BTU/h 751 BTU/h 785 BTU/h

Max Speed n

max

14 800 rpm
(Depending on
Rotor)

Min Speed n

min

Maximum RCF Value at n

max

300 rpm 300 rpm 300 rpm

21 100 x g 21 100 x g 21 100 x g

Maximum Kinetic Energy <2,35 kNm <2,35 kNm <2,35 kNm

Noise Level at maximum Speed < 50 dB (A) < 50 dB (A) < 50 dB (A)

Dimensions

Hight
Width
Depth

230 mm
240 mm
350 mm

Weight without Rotor 10.5 kg 10.5 kg 10.5 kg

Directives, Standards and Guidelines

Table 1-3. Directives, Standards and Guidelines

Tension /
Frequency

230V
50Hz/60Hz,

Marked

EC:

98/79/EC In-vitro-Diagnostica (IvD)

2006/42/EC Machine Directive

2004/108/EC EMC Directive

2006/95/EC Low Voltage Directive

100V 50Hz cCSAus: UL Std. No. UL 61010-1, 2

Produced and inspected according to the following standards
and guidelines

EN 61010-1, 2

EN 61010-2-020, 2

nd

Edition

nd

Edition

EN 61010-2-101

EN 61326-1

EN 61326-2-6

EN 55011B

EN 61000-6-2

EN ISO 13485

CAN/CSA-C22.2 No. 61010-1-04

CAN/CSA-C22.2 No. 61010-2-020-09 Part 2-020:

IEC 61010-2-020 2

nd

Edition

(Pollution degree 2, Overvoltage category II)

IEC 61010-2-101

14 800 rpm
(Depending on
Rotor)

230 mm
240 mm
350 mm

nd

Edition

14 800 rpm
(Depending on
Rotor)

230 mm
240 mm
350 mm

1-4 Heraeus Fresco 17 / 21 Thermo Scientific

1

Introduction and Description

Functions and Features

Functions and Features

The following table contains an overview of the electrical connection data for the Heraeus Fresco 17 /

21.

Table 1-4 . Functions and Features

Component / Function Description / Features

Structure / Housing Sheet steel chassis with attached plastic housing and steel

reinforcement

Rotor Chamber Plastic

Up to 48 ml of spilled liquids are retained in the chamber and cannot
enter the instrument.

Drive Induction drive without carbon brushes

Keys and Display Easy-to-clean keypad and display surface

Controls Microprocessor-controlled

Internal Memory Most recent data is saved

Functions RCF-selection, temperature control, pretemp

Imbalance Recognition Electronic in ventilated centrifuges

Door Lock Automatic door closing and locking starting from an initial hold

position

Mains Supply

The following table contains an overview of the electrical connection data for the der Heraeus Fresco
17 / 21.This data is to be taken into consideration when selecting the mains connection socket.

Table 1-5 . Electrical connection data

Cat.

75002420 FRESCO 17 230 V 50 / 60 Hz 1.9A 330 W 4A, 2-pole circuit breaker 16 AT

75002421 FRESCO 17 120 V 60 Hz 2.2 A 330 W 8A, 2-pole circuit breaker 15 AT

75002422 FRESCO 17 100 V 60 Hz 2.4 A 330 W 8A, 2-pole circuit breaker 16 AT

75002425 Fresco 21 230 V 50 / 60 Hz 2.2 A 370 W 4A, 2-pole circuit breaker 16 AT

75002421 Fresco 26 120 V 60 Hz 4.4 A 370 W 8A, 2-pole circuit breaker 15 AT

75002422 Fresco 27 100 V 60 Hz 5.1 A 360 W 8A, 2-pole circuit breaker 16 AT

Mains
voltage

Frequency

Rated
current

Power
consumption

Equipment fuse Building fuse

Thermo Scientific Heraeus Fresco 17 / 21 1-5

1

Introduction and Description

Rotor Selection

Rotor Selection

The Heraeus Fresco 17 / 21 is supplied with a rotor

Various rotors are available to choose from.

Microliter 24 x 2 7500 3424

Microliter 36 x 0.5 7500 3436

Dual Rotor 18x2 / 0.5 ml 7500 3418

PCR Rotor 4x8 7500 3440

PCR Rotor 8x8 7500 3489

Hematocrit Rotor 7500 3473

The technical data of the rotors and the corresponding adapters and reduction sleeves for various
commercially available containers can be found in the corresponding rotor operating manuals.

For more information visit our website at: www.thermoscientific.com/centrifuge.

1-6 Heraeus Fresco 17 / 21 Thermo Scientific

2

Before Use

Contents

• “Before Setting up” on page 2-2

• “Transporting the Centrifuge” on page 2-2

• “Location” on page 2-2

• “Aligning the Centrifuge” on page 2-3

• “Mains Connection” on page 2-3

• “Storage” on page 2-4

Thermo Scientific Heraeus Fresco 17 / 21 2-1

2

Before Use

Before Setting up

Before Setting up

1. Check the centrifuge and the packaging for any shipping damage.
Inform the shipping company and Thermo Fisher Scientific immediately if any damage is
discovered.

2. Remove the packaging.

3. Remove the rotor transport protection. The cardboard shall prevent damages due to impacts.

4. Check the order for completeness (see “Scope of Supply” auf Seite iii).
If the order is incomplete, please contact Thermo Fisher Scientific.

Transporting the Centrifuge

• Due to its weight (see “Technical Data” on page 1-3), the centrifuge should be carried by several

people.

• Always lift the centrifuge at both sides on the bottom plates.

Location

Figure 2-1.

• The centrifuge can get damaged due to impacts.

The centrifuge should only be operated indoors.

The set-up location must fulfil the following requirements:

• A safety zone of at least 30 cm must be maintained around the centrifuge.

People and hazardous substances must be kept out of the safety zone while centrifuging.

• The supporting structure must be stable and free of resonance, for example a level laboratory

bench.

• The supporting structure must be suitable for horizontal setup of the centrifuge.

• The centrifuge should not be exposed to heat and strong sunlight.

Lifting the centrifuge at both sides.

WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.

2-2 Heraeus Fresco 17 / 21 Thermo Scientific

• The set-up location must be well-ventilated at all times.

Main Power Switch

Mains plug

Aligning the Centrifuge

The horizontal alignment of the centrifuge must be checked every time after moving it to a different
location.
The supporting structure must be suitable for horizontal setup of the centrifuge.

CAUTION If the centrifuge is not level, imbalances can occur and the centrifuge can be
damaged.
Do not place anything under the feet to level the centrifuge.

Mains Connection

2

Before Use

Aligning the Centrifuge

Figure 2-2. Mains Connection

1. Turn off the power supply switch on the back (press "0").

2. Plug the centrifuge into grounded electrical sockets only.

Note The centrifuge must be connected directly to a socket. Extensions and conneters are not

allowed.

3. Check whether the cable complies with the safety standards of your country.

4. Make sure that the voltage and frequency correspond to the figures on the rating plate.
Establish the connection to the power supply with the connecting cable.

Thermo Scientific Heraeus Fresco 17 / 21 2-3

2

Before Use

Storage

Storage

• Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and

decontaminated.

• Store the centrifuge in a clean, dust-free location.

• Be sure to place the centrifuge on its feet.

• Avoid direct sunlight.

2-4 Heraeus Fresco 17 / 21 Thermo Scientific

3

Control Panel

Contents

• “Control Panel” on page 3-2

• “Keys” on page 3-2

Thermo Scientific Heraeus Fresco 17 / 21 3-1

3

PULSE

OPEN

STOP

START

Running time

Speed / RCF Value

Temperature

Toggle key

Prewarming
or Precooling

Control Panel

Control Panel

Control Panel

In the controll panel you find the keys and displays of the centrifuge. The main power switch is located
on the left side. All parameters can be selected and changed during operation.

Keys

The keys allow user intervention for controlling the operating mode as follows:

Key Display contents

Start

Stop

Open lid

Pulse

PreTemp

Normal start of the centrifuge

End run manually

Automatic release (possible only when device is switched on). Emergency
release (see “Mechanical Emergency Door Release” on page 6-2)

By pressing the key the centrifuge starts immediately and
accelerates up to the end speed. Releasing the key initiates a stopping
process at the highest braking curves.

By pressing the key you start the pretemp function of the centrifuge.

By pressing the key up and down you change the value in the
display. If you hold the key pressed, the display changes continuously at
first slowly and after a few seconds at an accelerated pace to the higher
or lower values. By pressing the key briefly, you increase or decrease the
speed in one step. When pressing both arrows the cursor moves to the
left..

3-2 Heraeus Fresco 17 / 21 Thermo Scientific

Changing the display
mode

Use the key to change the display mode. (speed / RCF value, sample /
chamber temperature, run time counter from start or preset speed on)

4

Operation

Contents

• “Switch on Centrifuge” on page 4-2

• “Door Opening” on page 4-2

• “Close Door” on page 4-2

• “Rotor Installation” on page 4-2

• “Entering Parameters” on page 4-4

• “Centrifugation” on page 4-8

• “Short-term Centrifugation” on page 4-8

• “Removing the Rotor” on page 4-9

• “Aerosol-tight Rotors” on page 4-9

• “Audible Alarm” on page 4-9

• “Turning off the Centrifuge” on page 4-10

Thermo Scientific Heraeus Fresco 17 / 21 4-1

4 Operation

Switch on Centrifuge

Switch on Centrifuge

1. Switch on the centrifuge.

The centrifuge shows the actual value in the display. Speed and Run time show 0. The display
shows the current temperature of the sample.

Door Opening

1. Press the key.
The display shows the following message:

Note Use the emergency release only for malfunctions and power failures (see “Mechanical

Emergency Door Release” on page 6-2).

Close Door

1. Close the door by pressing down on it lightly in the middle or on both sides of it.

Note The door should audibly click into place.

CAUTION Do not slam the door.

Rotor Installation

The approved rotors for the Heraeus Fresco 17 / 21 are listed in section “Rotor Selection” on page 1-6.
Use only the rotors and accessories from this list in the centrifuge.

CAUTION Unapproved or incorrectly combined accessories can cause serious damage to
the centrifuge.

Proceed as follows:

1. Open the door of the centrifuge and if necessary remove any dust, foreign objects or residue from
the chamber.
Thread and o-ring must be clean and undamaged.

4-2 Heraeus Fresco 17 / 21 Thermo Scientific

4 Operation

Rotor Installation

CAUTION Do not install the rotor when the temperature difference between shaft and
rotor lock is >20°C. Otherwise the rotor might jam during the installation. A jammed
rotor can lead to damages of centrifuge and rotor.

2. Hold the rotor over the centrifuge spindle. The two bars in the labeling on the upper side of the
rotor (a) must be above the retaining pin (b) of the centrifuge spindle.

a

b

3. Let the rotor slide slowly down the centrifuge spindle.

CAUTION Do not force the rotor onto the centrifuge spindle.
If the rotor is very light, then it may be necessary to press it onto the centrifuge spindle
with a bit of pressure.

4. Thread the fastening tool into the centrifuge spindle clockwise. Hold the rotor with the other hand
into position.

5. Close the rotor.

6. Check if the rotor is properly installed by lifting it slightly on the handle.

Thermo Scientific Heraeus Fresco 17 / 21 4-3

4 Operation

Speed display

RCF display

Entering Parameters

WARNING Check for any damage to the rotor: Damaged rotors must not be used.
Keep the centrifuge spindle area of the rotor clear of objects.

CAUTION Check that the rotor is properly locked on the centrifuge spindle before each
use by pulling it at its handle. Fasten the rotor if necessary.

Be sure to check all sealings before starting any aerosol-tight applications.

See the information in the rotor instruction manual.

7. Close the centrifuge door.

Entering Parameters

Note Due to limited display digits there is a need to round the values. The direct comparison

between the two values speed and RCF is therefore restricted.

Select Speed or RCF-Value

1. Press the key under the left display. When the lower indicator is lit the display shows the speed.
Press the key to toggle between the two modes.

2. When the upper indicator is lit the display shows the RCF value. Press the key to toggle
between the two modes.

4-4 Heraeus Fresco 17 / 21 Thermo Scientific

4 Operation

RCF 11 18,

n

1000

-------------



2

r××=

Entering Parameters

Pre-selecting Speed

1. Enter the desired value by pressing the key repeatedly, until the desired value shows.
You can adjust the speed in 100 rpm increments.

2. Press the key to confirm the pre-selected value.
If you do not press any key, the display flashes for a few seconds The new preselected value is now
stored and the display shows the actual value.

Note The centrifuge speed can be set to a minimum of 300rpm.
The maximum speed depends on the centrifuge variant.

Pre-selecting RCF-Value

1. Enter the desired value by pressing the key repeatedly, until the desired value shows.
You can adjust the RCF pre-selected value in steps of 100 xg.

2. Press the key to confirm the pre-selected value.
If you do not press any key, the display flashes for a few seconds The new preselected value is now
stored and the display shows the actual value.

Note The RCF value can be set to a minimum of 100 xg.
The maximum speed depends on the centrifuge variant.
The displayed RCF value is always corresponding to the maximum of centrifuge radius of the
micro liter rotor 24 x 2 (75003424).
Refer to the “Explanation of RCF-Value” on page 4-5 and the rotor manual for further
information.

Explanation of RCF-Value

The relative centrifugal force (RCF) is given as a multiple of the force of gravity g. It is a unitless
numerical value which is used to compare the separation or sedimentation capacity of various
centrifuges, since it is independent of the type of device. Only the centrifuging radius and the speed
come into play in it:

r = centrifuging radius in cm

n = rotational speed in rpm

The maximum RCF value is related to the maximum radius of the tube opening.

Thermo Scientific Heraeus Fresco 17 / 21 4-5

Remember that this value is reduced depending on the tubes and adapters used.

This can be accounted for in the calculation above if required.

4 Operation

Entering Parameters

Run Time Pre-selection

Note You can select a run time between 1 and 99 min or continuous operation.

1. Enter the desired value by pressing the key below the display in the middle repeatedly, until
the desired value shows.
You can adjust the run time in steps of 1 min.

2. Press the key to confirm the pre-selected value.
If you do not press any key, the display flashes for a few seconds The new preselected value is now
stored and the display shows the actual value.

Continuous Operation

1. Press the key until hd is shown.

2. During continuous operation, the centrifuge will continue running until you stop it manually with
the key.

CAUTION Please note that the lifetime of particularly plastic rotor tubes is limited.
Continuous operation (extended use) may cause damage to them.

Pre-selecting the Temperature

You can determine the sample temperature in °C. Proceed as follows:

1. Enter the desired value by pressing the key under the right display repeatedly, until the
desired value shows.
You increase or decrease the temperature in steps of 1°C.

2. Press the key to confirm the pre-selected value.
If you do not press any key, the display flashes for a few seconds The new preselected value is now
stored and the display shows the actual value.

4-6 Heraeus Fresco 17 / 21 Thermo Scientific

4 Operation

Entering Parameters

3. Close the centrifuge door.

4. Restart the centrifuge.
The refrigeration starts operating if the pre-selected temperature is below the current temperature
of the rotor chamber.

Pre-warming or Pre-cooling the Centrifuge

In order to pre-temp the centrifuge and the unloaded rotor proceed as follows:

1. Press the key.
An indicator above the key indicates operation at the activated pre-temp function.

2. Enter the desired value by pressing the key under the right display repeatedly, until the
desired value shows.
You increase or decrease the temperature in steps of 1°C.

3. Press the key.
The rotor will be operated at optimal speed.

Note When you press a different key than the key you will quit the pre-temp function.

Note If you wish to change the temperature of your samples, please consider that the time required

for temperature adjustment is prolonged. For critical applications you should take other precautions
to ensure that the desired temperature is actually reached and maintained.

Changing the settings during the run

You can change the settings during the run as follows:

1. Press one of the three
The current value will switch into the pre-selecting value mode.

2. Enter the new value as described above.

keys in the control panel.

Thermo Scientific Heraeus Fresco 17 / 21 4-7

4 Operation

Centrifugation

3. Press the key.
The value is taken over immediately.

Centrifugation

Once the rotor has been properly installed, the main switch has been turned on and the door has been
closed, you can start centrifuging.

Starting Centrifuge Program

Press the key on the control panel. The centrifuge accelerates to the preset speed with the time
display active.

The run display begins to count down from the pre-selected value. If the remaining run time is less
then 1 minute, the remaining time is given in seconds.

The circulating indicators in the left display represent the spinning rotor.

In continuous operation hd the time display counts up. The displayed run time is first in seconds. After
one minute the displays changes every minute.

Stopping the Centrifugation Program

With preset Run Time

If the run time has been pre-selected, and all you have to do, is to wait until the centrifuge terminates
the run automatically.

As soon as the speed drops to zero, the message END will appear in the display. By pressing the
key, you can open the door and remove the centrifuge material.

You can also stop the centrifuging program manually at any time by pressing the key.

Continuous Operation

If you selected continuous operation (see “Continuous operation” on page 4-6), you will have to stop
the centrifuge manually.

1. Press the key on the control panel.
The centrifuge will be decelerated at the designated rate.

2. Press the key to open the centrifuge door and the remove the samples when the message END
appears in the display.

Short-term Centrifugation

For short-term centrifuging, the Heraeus Fresco 17 / 21 has a PULSE- function.

By holding down the key, spinning will start and continue until the key is let go.

The centrifuge accelerates and brakes at maximum power. The pre-selected value is ignored.

4-8 Heraeus Fresco 17 / 21 Thermo Scientific

Note The centrifuge accelerates to the maximum speed.

Check carefully whether you have to maintain a certain speed for your application.

The displayed run time is first in seconds. After one minute the displays changes every minute.

After an short-term centrifugations the set values are restored.

Removing the Rotor

To remove the rotor, proceed as follows:

1. Open the centrifuge door.

2. Unscrew the rotor with the allen wrench.

3. Grab the rotor in the middle. Pull the rotor directly upwards and remove it from the centrifuge
spindle. Make sure not to tilt the rotor while doing this.

4 Operation

Removing the Rotor

WARNING Be careful, when you change a rotor after a run. Centrifuge spindle and
motor bearing assembly can be hot (>55°C).

Aerosol-tight Rotors

When using an aerosol tight lid the rotor can be removed with the lid closed. This is to protect you and
the samples.

Audible Alarm

Error

Accompanying all error messages, a warning signal is given out.

Press any key to silence the warning signal.

End of Run

By default there is an acoustic signal at the end of any centrifugation run. To switch off this signal
proceed as follows:

Press the key when you turn on the centrifuge.
The display shows:

Thermo Scientific Heraeus Fresco 17 / 21 4-9

4 Operation

Turning off the Centrifuge

or

4. Press the key under the display in the middle.
The acoustic signal is turned on or off.

5. Press the key to confirm the pre-selected value.

Turning off the Centrifuge

1. To turn off the centrifuge put the mains switch to "0".

Note The centrifuge is equipped with a special switch for balancing potential voltage discrepancies
in the power grid. After pressing the mains switch the display therefore may still flash up to 10
seconds.

4-10 Heraeus Fresco 17 / 21 Thermo Scientific

5

Maintenance and Care

Contents

• “Cleaning Intervals” on page 5-2

• “Cleaning” on page 5-2

• “Disinfection” on page 5-4

• “Decontamination” on page 5-5

• “Autoclaving” on page 5-5

• “Service of Thermo Fisher Scientific” on page 5-6

Thermo Scientific Heraeus Fresco 17 / 21 5-1

5



Maintenance and Care

Cleaning Intervals

Cleaning Intervals

For the sake of personal, environmental, and material protection, it is your duty to clean and if
necessary disinfect the centrifuge on a regular basis.

Maintenance Recommended Interval

Clean Rotor Chamber Daily or when polluted

Clean Rotor Daily or when polluted

Accessories Daily or when polluted

Cabinet Once per Month

Filter Unit Every three Months

Ventilation Holes Every six Months

CAUTION Refrain from using any other cleaning or decontamination procedure than those
recommended here, if you are not entirely sure that the intended procedure is safe for the
equipment.
Use only approved cleansers.
If in doubt, contact Thermo Fisher Scientific.

Cleaning

When cleaning centrifuge

• Use warm water with a neutral solvent.

• Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions or

scrubbing powder.

• Rinse the cavities out thoroughly.

• Use a soft brush without metal bristles to remove stubborn residue.

• Afterwards rinse with distilled water.

• Place the rotors on a plastic grate with their cavities pointing down.

• If drying boxes are used, the temperature must never exceed 50°C, since higher temperatures could

damage the material and shorten the lifetime of the parts.

• Use only disinfectants with a pH of 6-8.

• Dry aluminum parts off with a soft cloth.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000

9824). Also treat the cavities with oil.

• Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing

down.

5-2 Heraeus Fresco 17 / 21 Thermo Scientific

5 

Maintenance and Care

CAUTION Before using any cleaning or decontamination methods except those

recommended by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment.

Clean centrifuge and accessories as follows:

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

5. Remove the centrifuge tubes and adaptors.

6. Use a neutral cleaning agent with a pH value between 6 and 8 for cleaning.

7. Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a
maximum temperature of 50°C.

Cleaning

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000

9824). Also treat the cavities with oil.

After some applications their might be ice in the rotor chamber. Let the ice melt and drain it off. Clean
the rotor chamber as described above.

Cleaning Filter Unit

Refrigerated centrifuges have a filter unit to protect the cooling device.

CAUTION When cleaning, do not allow liquids, especially organic solvents, to get on the
drive shaft or the bearings of the centrifuge.
Organic solvents break down the grease in the motor bearing. The drive shaft could freeze
up.

1. Pull the centrifuge to the edge of the table.

2. Pull the clip below the sucking grid and remove the filter unit completely by pulling it down.

3. Remove the cumulated dust with a soft cloth.

Thermo Scientific Heraeus Fresco 17 / 21 5-3

5



Maintenance and Care

Disinfection

4. Inserting the filter again The labelling Front must show to the front of the centrifuge.

5. Push the filter upwards into the slot until the clip locks in the bottom plate.

Disinfection

Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation.

The rotor chamber and the rotor should be treated preferably with a neutral disinfectant.

WARNING Infectious material can get into the centrifuge when a tube breaks or as a
result of spills. Keep in mind the risk of infection when touching the rotor and take all
necessary precautions.
In case of contamination, make sure that others are not put at risk.
Decontaminate the affected parts immediately.
Take other precautions if need be.

CAUTION Before using any cleaning or decontamination methods except those
recommended by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment.
Observe the safety precautions and handling instructions for the cleaning agents used.

Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other
disinfectants.

Disinfect the rotor and accessories as follows:

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

5. Remove the centrifuge tubes and adaptors and dispose of them or disinfect them.

6. Treat the rotor and accessories according to the instructions for the disinfectant. Adhere strictly to
the given application times.

7. Be sure the disinfectant can drain off the rotor.

8. Rinse the rotor and accessories thoroughly with water.

9. Dispose of the disinfectant according to the applicable guidelines.

10. Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a
maximum temperature of 50°C.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000

9824). Also treat the cavities with oil.

5-4 Heraeus Fresco 17 / 21 Thermo Scientific

Decontamination

Decantaminate the centrifuge immediately whenever radioactive material has spilled during
centrifugation.

WARNING Infectious material can get into the centrifuge when a tube breaks or as a
result of spills. Keep in mind the risk of infection when touching the rotor and take all
necessary precautions.
In case of contamination, make sure that others are not put at risk.
Decontaminate the affected parts immediately.
Take other precautions if need be.

CAUTION Before using any cleaning or decontamination methods except those
recommended by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment.

For general radioactive decontamination use a solution of equal parts of 70% ethanol, 10% SDS and
water.

5 

Maintenance and Care

Decontamination

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

5. Remove the centrifuge tubes and adaptors and dispose of them or disinfect them.

6. Rinse the rotor first with ethanol and then with de-ionized water.

• Adhere strictly to the given application times.

7. Be sure the decontamination solution can drain off the rotor.

8. Rinse the rotor and accessories thoroughly with water.

9. Dispose of the decontamination solution according to the applicable guidelines.

10. Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a
maximum temperature of 50 °C.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000

9824). Also treat the cavities with oil.

Autoclaving

1. Before autoclaving clean rotor and accessories and described above.

2. Place the rotor on a flat surface.

• Rotors and adapter can be autoclaved at 121°C.

• The maximum permissible autoclave cycle is 20 minutes at 121°C.

Note No chemical additives are permitted in the steam.

Thermo Scientific Heraeus Fresco 17 / 21 5-5

5



Maintenance and Care

Service of Thermo Fisher Scientific

CAUTION Never exceed the permitted temperature and duration when autoclaving.
If the rotor shows signs of corrosion or wear, it must be replaced.

Service of Thermo Fisher Scientific

Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an
authorized service technichian. The service technicians check the following:

• the electrical equipment

• the suitability of the set-up site

• the lid lock and the safety system

• the rotor

• the fixation of the rotor and the drive shaft

Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are
performed for free during the warranty period and afterwards for a charge.
This is only valid if the centrifuge has only been mainained by a Thermo Fisher Scientific service
technician.

Shipping and Deposing of Accessories

WARNING Before shipping or deposing centrifuges and accessories you have ton clean

and if necessary desinfect or decontaminate everything. Use the card in annex

“DECONTAMINATION INFORMATION CERTIFICATE” on page C-1

5-6 Heraeus Fresco 17 / 21 Thermo Scientific

6

Troubleshooting

Contents

• “Mechanical Emergency Door Release” on page 6-2

• “Troubleshooting by User” on page 6-3

• “When to contact Service Technician” on page 6-5

Thermo Scientific Heraeus Fresco 17 / 21 6-1

6

Emergency Door Release



Troubleshooting

Mechanical Emergency Door Release

Mechanical Emergency Door Release

During a power failure, you will not be able to open the centrifuge door with the regular electric door
release. A mechanical override is provided to allow sample recovery in the case of an emergency.
However, this should be used only in emergencies and after the rotor has come to a complete stop.

WARNING The rotor can still be spinning at high speed. If touched, it can cause serious
injuries.

Always wait a few minutes until the rotor has come to a stop without braking. The brake does not work
when there is no current. The braking process lasts much longer than usual.

Proceed as follows:

1. Make sure the rotor has stopped (view port in the lid).

WARNING Never use your hand or other tools to brake the rotor.

2. Pull out the power supply plug.

3. Insert a 3 inch long wire (e.g. a staple) into the hole above the control panel.

4. Press the centrifuge door down gently. Push the wire further into the hole until you hear and feel
the door latch unlocking.

5. Remove the wire from the hole and open the centrifuge door.
Now you can remove your samples.

Figure 6-1. Emergency Door Release

Reconnect the centrifuge once the power has been restored. Switch on the centrifuge.

6-2 Heraeus Fresco 17 / 21 Thermo Scientific

Troubleshooting by User

If problems occur other than those listed in this table, the authorized customer service
representative must be contacted.

Failure message Problem with centrifuge Possible causes and cures

6 

Troubleshooting

Troubleshooting by User

Display remains dark. The drive stops. The centrifuge runs down

without being braked. The centrifuge door
can not be opened.

Display fails briefly The drive stops. The centrifuge runs down

without being braked.

The centrifuge door
can not be opened.

- Exceptionally running noise. Imbalance

Pressing key has no effect. Centrifuge door is not correctly engaged or warped.

No mains connection

1. Is the centrifuge turned on?

2. Check the mains connection.

If an error message appears again, inform Customer Service.

Mains connection interrupted for some seconds,

1. Turn off mains swich.

2. Check whether the mains power cord is connected properly

3. Restart the centrifuge.

1. Check if mains connection is working and the instrument is
switched on (display is lit).

2. If this is unsuccessful, you may open the centrifuge door
using the mechanical emergency door release (“Mechanical

Emergency Door Release” on page 6-2)

1. Stop the centrifuge. Press the key or unplug mains power
cord.

2. Wait until the centrifuge comes to a complete stop.

3. Check whether the rotor is properly loaded.

Check whether a broken tube, damage to the rotor or motor is
responsible for the run noise.
If an error message appears again, inform Customer Service.

Display oP appears
although lid is closed.

Lid Rotor stops with deceleration to standstill. Centrifuge door was opened manually during the run. Close

E-01 - E-13 Rotor stops with deceleration to standstill.

Thermo Scientific Heraeus Fresco 17 / 21 6-3

Centrifuge does not start. Centrifuge door not properly closed. Open the centrifuge door

and repeat locking procedure.
If an error message appears again, inform Customer Service.

centrifuge door immediately

Rotor stops with deceleration to standstill.

For further centrifugation, you have to switch the instrument off
and switch it on again.

Internal program error

The centrifuge cannot be operated.

Switch the instrument off and on again.

If an error message appears again, inform Customer Service.

6



Troubleshooting

Troubleshooting by User

Failure message Problem with centrifuge Possible causes and cures

E-14 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

Overtemperature in the centrifuge chamber.

Switch the centrifuge off and turn it on again after approx. one
minute.

If an error message appears again, inform Customer Service.

E-15-E-16 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

Temperature measurement error

Switch the instrument off and on again.

If an error message appears again, inform Customer Service.

E-22 - E-23 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

Error in speed entry

Switch the instrument off and on again.

The display shows BR and a countdown from 100 - 0.

If an error message appears again, inform Customer Service.

E-24 The centrifuge cannot be operated. Wrong status information from the door latch

1. Switch the instrument off and on again.

2. After re-switching on, the display shows Lid FAiL.

3. If the centrifuge door has been already opened, the display
shows CLOSE Lid. Thereupon close the door.

4. The centrifuge tries to open the lid to switch for starting the
normal operation mode.

If an error message appears again, inform Customer Service.

E-29 Motor does not start Motor or rotor blocked

1. Switch instrument off and on again using the mains switch.

2. Open the centrifuge door.

3. Check whether the rotor can turn freely.

If an error message appears again, inform Customer Service.

E-31 Rotor stops without deceleration to

standstill or does not start.

Overtemperature in the motor

1. Turn instrument off and unplug mains power cord.

2. Check and clean the venting slots if necessary and
respectively the filter unit of the cooled centrifuge.

3. After approx. 60 minutes you can restart the instrument.

Observe the maximum permissible environmental temperature.

If an error message appears again, inform Customer Service.

E-33 Rotor stops with deceleration to standstill. Overpressure in the refrigeration system

1. Turn instrument off and unplug mains power cord.

2. Check and clean the venting slots if necessary and
respectively the filter unit of the cooled centrifuge.

3. After approx. 60 minutes you can restart the instrument.

Observe the maximum permissible environmental temperature.

If an error message appears again, inform Customer Service.

E-36 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

Overcurrent or error in current measurement

Switch the instrument off and on again.

If an error message appears again, inform Customer Service.

6-4 Heraeus Fresco 17 / 21 Thermo Scientific

Failure message Problem with centrifuge Possible causes and cures

6 

Troubleshooting

When to contact Service Technician

E-41 - E-56 Rotor stops with deceleration to standstill.

The centrifuge cannot be operated.

E-60 Rotor stops with deceleration. Insufficient temperature in the refrigeration unit.

Internal program error

Switch the instrument off and on again.

If an error message appears again, inform Customer Service.

1. Stop the centrifugation run.

2. Open the centrifuge door and defrost the chamber.
Never touch the chamber directly with your hands – you may
freeze up.

3. After approx. 60 minutes you can restart the instrument.

Observe the maximum permissible ambient temperature

4. If a strong ice sheet is present in the internal chamber, be
sure to remove all con¬densate after defrosting.

If an error message appears again, inform Customer Service.

When to contact Service Technician

If you need to contact customer service, please provide the order no. and the serial no. of your device.
This information can be found on the back near the inlet for the power supply cable.

To identify the software version, proceed as follows:

1. Push the key and then switch on the centrifuge.
In the display all segments are lit.

• Subsequently, the following readings will be displayed for 5 seconds each:

Software number SOFT 058 3_

Software version _01

NV-RAM number EEPRO 462 1_

NV-RAM version _01

Cycle counter CYCLE 001 25

• The values shown above are just examples.

Software 0583 Version 01

NV-RAM 4521 Version 01

cycles completed 125

2. Communicate the software version to the service technician.

Thermo Scientific Heraeus Fresco 17 / 21 6-5

A

Chemical Compatibility Chart

CHEMICAL

2-mercaptoethanol

Acetaldehyde

Acetone

Acetonitrile

Alconox

Allyl Alcohol

Aluminum Chloride

Formic Acid (100%)

Ammonium Acetate

Ammonium Carbonate

Ammonium Hydroxide (10%)

Ammoniumhydroxid (28 %)

Ammonium Hydroxide (conc.)

Ammonium Phosphate

Ammonium Sulfate

Amyl Alcohol

Aniline

Sodium Hydroxide (<1%)

Sodium Hydroxide (10 %)

Barium Salts

Benzene

Benzyl Alcohol

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

SSU- SMS- SUSSUSS- SSSSUSSSSSS

S-UU---M-U---MUUUMM- MSU- S- U

MSUUSUMS SUUSUSUUUSSUUSMMSUU

SSU- SMS- SSUSUMUU- SMUUSSSSUU

UUS- SSS- SSSSSSMSSSSSSSSSSSU

---U--S----S-SSMSSS-MS--S--

UUSSSSUSSSSMSSSS- SSSSSMUUSS

-SMU--U----U-SMUUSS-US-US-U

SSU- SSS- SSSSSSSU- SSSSSSSSSS

MSUSSSSSSSSSSSUU- SSSSSSMSSS

UUSUSSMSSSSS- SUMSSSSSSSSSMS

UUSUSUMSSSSSUSUMSSSSSSSSSMS

UUUUSUMS- S- SUSUUSSS- MSSSS- U

U- S- SSSSSSSS- SSM- SSSSSSMSSS

UMS- SSUSSSSSSSSS- SSSSSSUSSU

S- MU- - SS- M- S- MSSSSM---U-S-M

SSUUSUSMSUUUUUUU- S MUUSSSSUS

U- MSSS- - SMSS- SMMSSSSSSMSS- U

U- MU- - U- MMSSUSUUSSSSSSMSS- U

MUS- SSSSSSSSSSSM- SSSSSSMSSS

SSUUSUMUSUUSUUUMUMUUUSUUSUS

S- UU- - MM- M- SUUUUUUU- MSM- S- S

, POLYCLEAR, CLEARCRIMP

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

VITON

Thermo Scientific Heraeus Fresco 17 / 21 A-1

A



Chemical Compatibility Chart

CHEMICAL

Boric Acid

Cesium Acetate

Cesium Bromide

Cesium Chloride

Cesium Formate

Cesium Iodide

Cesium Sulfate

Chloroform

Chromic Acid (10%)

, POLYCLEAR, CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

USSMSSUSSSSSSSSSUSSSSSSSSSS

M- S- SSS- SSSS- SS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSSUSSS- SSSSSSS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

UUUUSSMUSUUMUMUUUMMUUSUUUMS

U- UUSUU- SSSUSSMUMSSUMSMUSSS

VITON

Chromic Acid (50%)

Cresol Mixture

Cyclohexane

Deoxycholate

Distilled Water

Dextran

Diethyl Ether

Diethyl Ketone

Diethylpyrocarbonate

Dimethylsulfoxide

Dioxane

Ferric Chloride

Acetic Acid (Glacial)

Acetic Acid (5%)

Acetic Acid (60%)

Ethyl Acetate

Ethyl Alcohol (50%)

Ethyl Alcohol (95%)

Ethylene Dichloride

Ethylene Glycol

Ethylene Oxide Vapor

Ficoll-Hypaque

U-UU-UU---SUUSMUMSSUMS-UM-S

SSU---S-SUUUUUU- - UU- USSSSUS

SSS- SSSUSUSSUUUMSMUMMSUMMUS

SSS- SSS- SSSSSSS- - SSSSSSSSSS

SSSSSSSSSSSSSSSSSSSSSSSSSSS

MSSSSSS- SSSSSSSSSSSSSSSMSSS

SSUUSSSUSUUSUUUUUUUUUSSSSMU

S- UU- - M- SU- S- MUUUMM- US- - SUU

SSU- SSS- SSUSUSU- - SSSMSSSSSS

SSUUSSS- SUSSUSUU- SSUUSSSSUU

MSUUSSMMSUUSUMUU- MMMUSSSSUU

UUS---MS-M-S-S---SS---MUS-S

SSUUSSUMSUSUUUUUMSUMUSUUS- U

SSMSSSMSSSSSMSSSSSSSMSSMSSM

S S U U S S U - S MS U U MU S MS MS MS MU S MU

MMUUSSMMSSUSUMUU- S SUUSMMSUU

SSSSSSMSSSSSUSUSSSSSSSSMSMU

SSSUSSMSSSSSUSU- SSSMSSSUSMU

S- UU- - SM- UUSUUUUUUU- USU- S - S

SSSSSSSSSSSS- SUSSSSSSSSMSMS

S- U- - U- - SU- S- SM- - SSSUSUSSSU

MSS- SSS- SSSS- SS- SSSSSSSMSSS

A-2 Heraeus Fresco 17 / 21 Thermo Scientific

CHEMICAL

Hydrofluoric Acid (10%)

Hydrofluoric Acid (50%)

Hydrofluoric Acid (conc.)

Formaldehyde (40%)

Glutaraldehyde

Glycerol

Guanidine Hydrochloride

Haemo-Sol

Hexane

A 

Chemical Compatibility Chart

, POLYCLEAR, CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

UUUM- - U- - UUS- SMUSSSSMSUUU- -

UUUU- - U- - UUUUSUUUSSMMSUUU- M

UUUU- UUM- UMUUMUUU- S- USUUU- -

MMMSSSSMSSSSMSSSUSSMSSSMSMU

SSSS- - S- SSSSSSS- - SSS- - SSS- -

MSS- SSSSSSSSSSSS- SSSSSSSSSS

UUS- SSS- SSSSSSS- - SSSSSSUSSS

SSS---S-SSSSSSS- - SSSSSSSSSS

SSS- SSS- SSUSUMUSSUSSMSUSSUS

VITON

Isobutyl Alcohol

Isopropyl Alcohol

Iodoacetic Acid

Potassium Bromide

Potassium Carbonate

Potassium Chloride

Potassium Hydroxide (5%)

Potassium Hydroxide (conc.)

Potassium Permanganate

Calcium Chloride

Calcium Hypochlorite

Kerosene

Sodium Chloride (10%)

Iodoacetic Acid

Carbon Tetrachloride

Aqua Regia

Solution 555 (20%)

Magnesium Chloride

Mercaptoacetic Acid

Methyl Alcohol

Methylene Chloride

Methyl Ethyl Ketone

- - MU- - SS- U- SUSSMSSS- SSS- S- S

MMMU SSSSSUSSUSUMSSSSSSSMMMS

SSM- SSS- SMSSMSS- MSSSSSMSSMM

USS- SSS- SSSSSSSSSSS- SSSMSSS

MUSSSSS- SSSSSSUSSSSSSSSSSSS

USS- SSSSSSSSSSS- SSSSSSSUSSS

UUSSSSM- SSSS- SUSSSSSSSMUMSU

UUMU- - M- MSS- UMUUUS M- MU- UU- U

SSS- SSS- SSSUSSSM- SMSUSSMSUS

MUSSSSSSSSSSSSMS- SSSSSSMSSS

M- U- SMMS- M- S- SMS- SSSMSMUS- S

SSS- SSSUSMUSUMMS- MMMSSUSSUS

S- SSSSSS- - - SSSSS- SSSS- SSM- S

U-SUSSS----SSSSS- SS- S- SSM- S

U U MS S U MU S U U S U MU S S MMS MMMMU S S

U-UU--U-----UUUUUUU-----S-M

SSS---S-SSSSSSS- - SSS- SSSSSS

MSS- SSSSSSSSSSSSSSSSSSSMSSS

USU- SMS- SMSUUUU- S UUSMSUSSSS

SSSUSSMSSSSSUSUMSSSSSSSMSMU

UUUUMSSUSUUSUUUUUMUUUSSMUSU

SSUUSSMSSUUSUSUUUSSUUSSSSUU

Thermo Scientific Heraeus Fresco 17 / 21 A-3

A



Chemical Compatibility Chart

CHEMICAL

Metrizamide

Lactic Acid (100%)

Lactic Acid (20%)

N-Butyl Alcohol

N-Butyl Phthalate

N, N-Dimethylformamide

Sodium Borate

Sodium Bromide

Sodium Carbonate (2%)

Sodium Dodecyl Sulfate

Sodium Hypochlorite (5%)

Sodium Iodide

Sodium Nitrate

Sodium Sulfate

Sodium Sulfide

Sodium Sulfite

Nickel Salts

Oils (Petroleum)

Oils (Other)

Oleic Acid

Oxalic Acid

Perchloric Acid (10%)

Perchloric Acid (70%)

Phenol (5%)

Phenol (50%)

Phosphoric Acid (10%)

Phosphoric Acid (conc.)

Physiologic Media (Serum, Urine)

Picric Acid

Pyridine (50%)

Rubidium Bromide

, POLYCLEAR, CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

MSS- SSS- SSSS- SS- - SSSSSSMSSS

--S------MSU-SSSMSS- MSMSS- S

--SS-----MSM-SSSSSSSMSMSS- S

S- SU- - S- - SM- USMSSSSMMSM- S- S

SSU- SSS- SUUSUUUM- UUSUSMMSUS

SSSUSMS- SSUSUSUU- SSUUSMSSSU

MSSSSSSSSSSUSSSS- SSSSSSMSSS

USS- SSS- SSSSSSSS- SSSSSSMSSS

MUSSSSSSSSSSSSUSSSSSSSSSSSS

SSS- SSS- SSSSSSS- SSSSSSSSSSS

UUMSSMUSSMSSSMSSSSMSSSMUSMS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

SSS- SSSSSSSSSSSS- SSSSSUSSSS

USS- SSSSSSSSSSSSSSSSSSSMSSS

S-SS---S---SSSUU- - S- - - SSM- S

SSS- SSSSMSSSSSSM- SSSSSSSSSS

USSSSS- SSS- - SSSS- SSSSSSMSSS

SSS---SUSSSSUUMSMUUSSSUSSSS

S-S---SMSSSSUSSSSUSSSS- SSMS

S- USSSUUSUSSMSSSSSSSSSMUSMM

UUMSSSUSSSSSUSUSSSSSSSSUMSS

U- U- SUU- SMM- - MUMSMM- MSU- S- S

UUU- - UU- S UMUUMUUUMMUMSUUS US

U S U - S MM- S U MU U S U MS MS U U S U MMMS

USU- SUM- SUMUUUUUSUMUUSUUUMS

UUMSSSUSSSSU- SSSSSSSSSUMUSS

U U MM- - U S - MS U U MMS S S MS MS U MU - S

MSSS- - S- SSSSSSSSSSSSSSSSSSS

SSU- SMSSSMSUSSSUSSSSUSUMSMS

USUUSUU- US SUUMUU- USMUSSUUUU

MSS- SSS- SSSSSSS- - SSSSSSMSSS

VITON

A-4 Heraeus Fresco 17 / 21 Thermo Scientific

CHEMICAL

Rubidium Chloride

Sucrose

Sucrose, Alkaline

Sulfosalicylic Acid

Nitric Acid (10%)

Nitric Acid (50%)

Nitric Acid (95%)

Hydrochloric Acid (10%)

Hydrochloric Acid (50%)

Sulfuric Acid (10%)

Sulfuric Acid (50%)

Sulfuric Acid (conc.)

Stearic Acid

Tetrahydrofuran

Toluene

Trichloroacetic Acid

Trichloroethane

Trichloroethylene

Trisodium Phosphate

Tris Buffer (neutral pH)

Triton X-100

Urea

Hydrogen Peroxide (10%)

Hydrogen Peroxide (3%)

Xylene

Zinc Chloride

Zinc Sulfate

Citric Acid (10%)

1

Polyethyleneterephthalate

A 

Chemical Compatibility Chart

, POLYCLEAR, CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSS- SSSSSSSSSSSSSSSSSSSSSSS

MSS- SSS- SSSSSSUSSSSSSSSMSSS

UUSSSSS- SSSUSSS- SSS- SSSUSSS

USUSSUU- SUSU- SSSSSSSSSMSSSS

USUMSUU- SUSUUMMUMMMSSSUSSMS

U- UU- UU- - UUUUMUUUUMUUSUSS- S

UUMSSSU- SSSUUSUSSSSSSSSUMSS

UUUUSUU- SMSUUMUUSSSSMSMUUMM

MUUSSUU- SSMUSSSSSSSSSSUUUSS

MUUUSUU- SSMUUSUUMSSSSSUUUMS

MUUU- UUM- - MUUSUUUMSUMSUUU- S

S-S---SMSSSS- SSSSSSSSSMMSSS

SSUUSUUMSUUSUUU- MUUUUSUSSUU

SSUUSSMUSUUSUUUSUMUUUS USUUM

UUU- SSUMSUSUUSM- MSSUUSUUUMU

S-U---MU-U-SUUUUUUUUUSU- S- S

--UU---U-U-SUUUUUUUUUSU- U- S

---S--M------S--SSS--S--S-S

USSSSSS- SSSSSSSSSSSSSSSSSSS

SSS- SSS- SSSSSSSSSSSSSSSSSSS

S- USSSS----SSSMSSSS- SSSMS- S

UUMSSUU- SSSUSSSMUSSSSSSMSUS

SMSSS- S- SSSSSSSSMSSSSSSSSSS

SSUSSSMUSUUUUUUMUMUUUSUMSUS

UUSSSSUSSSSSSSSSSSSSSSSUSSS

USS- SSSSSSSSSSSSSSSSSSSSSSS

MSSMSSMSSSSSSSSSMSSSSSSSSSS

VITON

Thermo Scientific Heraeus Fresco 17 / 21 A-5

A



Chemical Compatibility Chart

Key

S Satisfactory

M Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.

U Unsatisfactory, not recommended.

-- Performance unknown; suggest testing, using sample to avoid loss of valuable material.

Chemical resistance data is included only as a guide to product use. No organized chemical resistance
data exists for materials under the stress of centrifugation. When in doubt we recommend pretesting
sample lots.

Suggest testing under actual conditions of use.

A-6 Heraeus Fresco 17 / 21 Thermo Scientific

B

Contact Information

United States / Canada 866-9-THERMO

Austria [phone]

Belgium [phone]

Germany 08001 536 376

[phone]

[phone]

France [phone]

Italy [phone]

Netherlands [phone]

Nordic / Baltic Countries / CIS [phone]

Russia [phone]

Spain / Portugal [phone]

Thermo Scientific Heraeus Fresco 17 / 21 B-1

B



Contact Information

Switzerland [phone]

UK / Ireland [phone]

China [phone]

[phone]

India 1800 22 8374

[phone]

Japan [phone]

Other Asian Countries [phone]

Australia [phone]

New Zealand [phone]

Other Countries [phone]

B-2 Heraeus Fresco 17 / 21 Thermo Scientific

I

Index

A

Accident Prevention ............................................. iv

Aerosol-tight Rotors ........................................... 4-9

Aligning ...........................................................2-3

Audible Alarm ................................................... 4-9

Autoclaving .......................................................5-5

B

Before Use ........................................................2-1

C

Care .................................................................5-1

Centrifugation ...................................................4-8

Characteristics ................................................... 1-2

Chemical Compatibility Chart ........................... A-1

Cleaning ........................................................... 5-2

Cleaning Intervals .............................................. 5-2

Close Door .......................................................4-2

Contact Information ..........................................B-1

Control Panel ....................................................3-2

D

Decontamination .............................................. 5-5

Deposing .......................................................... 5-6

Description .......................................................1-1

Directives .........................................................1-4

Disinfection ...................................................... 5-4

E

Emergency Door Release ....................................6-2

I

Intended Use ....................................................... iv

Introduction .....................................................1-1

K

Keys ................................................................. 3-2

L

Location ...........................................................2-2

M

Mains Connection .............................................2-3

Mains Supply .................................................... 1-5

Maintenance .....................................................5-1

O

Opening ...........................................................4-2

Operation .........................................................4-1

P

Parameters ........................................................4-4

Precautions .......................................................... v

Preface ............................................................... iii

R

RCF-Value .......................................................4-5

Removing the Rotor ...........................................4-9

Rotor Selection ..................................................1-6

F

Features ............................................................ 1-5

Filter Unit ........................................................5-3

Functions .........................................................1-5

G

GuidelineS ........................................................ 1-4

Thermo Scientific Heraeus Fresco 17 / 21 I-1

Scope of Supply ................................................... iii

Service ..............................................................5-6

Service Technician .............................................6-5

Setting up .........................................................2-2

Shipping ...........................................................5-6

Standards ..........................................................1-4

Storage .............................................................2-4

Switch on .........................................................4-2

S

Index:

T

Technical Data ..................................................1-3

Transporting ..................................................... 2-2

Troubleshooting .........................................6-1, 6-3

Turning off .....................................................4-10

2 Heraeus Fresco 17 / 21 Thermo Scientific