Thermo Heraeus Fresco 17, Heraeus Fresco 21 User manual

Thermo Scientific

Heraeus Fresco 17 / 21

Instruction Manual

50133376-2 August 2012

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Please reference this page for the most up-to-date information on the

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Heraeus Holding GmbH licensed to Thermo Fisher Scientific. Delrin, TEFLON, and Viton are

registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd. Hypaque is a registered trademark of Amersham Health As. RULON A and Tygon are registered trademarks of Saint-Gobain Performance Plastics. Alconox is a registered trademark of Alconox. Ficoll is a registered trademark of GE Healthcare. Haemo-Sol is a registered trademark of Haemo-Sol. Triton X-100 is a registered trademark of Sigma-Aldrich Co. LLC. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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ThermoFisher

SCIENTIFIC

KON FORM ITATSE RKLARU

DECLARATION

Name

für die Zusammenstellung der relevanten technischen

Name

representative

Anschrift

und

and address of the

des

to compile the

Herstellers

manufacturer and of the

CONFORMITY

und

relevant technical documentation:

des Bevollmächtigten

authorized

Thermo Electron LED GmbH Zweigniederlassu n

Osterode

Am Kalkberg

37520 Osterode

Germany

Hiermit erklären wir, Herewith

Besch rei bunq /descri otion

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we declare, that the

brand

dass die

Modellbezeichnunq / model name Modell Nr. / model no.

Gültig ab Equipmentnr.

Valid

from equipment no.

mit

allen einschlägigen Bestimmungen der

Übereinstimmung ist.

conformity with all relevant terms of directive

nachstehend beschriebene

machinery

described

Richtlinie über ln-vitro-Diagnostika

below

: Labor-Zentrifuqe / centrifuqe : Heraeus : FRESCO 17, FRESCO 21 : 75002420 75002423 75002425

: 41385539

for in vitro diagnostic medical

Unterlagen:

Maschine

(€

98/79/EG

devices 98n9/EC.

Die Maschine ist 2004n

0 8/EG ü be

The machinery is in accordance 2004/108/EC.

Die Schutzziele nach Anhang 1 der Maschinenrichtlinie

2006/9 s/EG we rden ei nge h alte n.

protection goals

The

met.

Angewandte harmonisierte Normen/

Harmonized standards used:

EN 61010-1:2004 EN 61010-2-020: 2006

EN 61010-2-101:2002 EN 61326-1: 2006 EN 61326-2-6: 2006 EN 5501 18:2007

Osterode. den O9.O7 .2012

,'+r--d.aqrz

Andreas Karl,

Dr.

Director R&D

Datum, Name, Funktion und Unterschrift

Ort, Place. date. name,

function

auch

Übereinstimmung

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ktrom

eti sc

with

Annex 1 for the directives

and

mit allen einschlägigen Bestimmungen

rt räg I i c h ke

all relevant terms

machinery 2006/42/EC

of directives for

2006/42/EG

Richtlinie

der

electromagnetic compatibility

und der Niederspannungsrichtlinie

low voltage 2006/95/EC

and

are

WEEE Conformity

This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96. It is marked by the following symbol:

Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device.

Table of Contents

Preface .......................................................................................................................................iii

Scope of Supply ....................................................................................................................... iii

Intended Use ..............................................................................................................................iv

Accident Prevention .................................................................................................................iv

Precautions .................................................................................................................................v

Chapter 1 Introduction and Description ...............................................................................................1-1

Characteristics of the Heraeus Fresco 17 / 21 ........................................................... 1-2

Technical Data ........................................................................................................ 1-3

Directives, Standards and Guidelines ...................................................................................... 1-4

Functions and Features ............................................................................................................ 1-5

Mains Supply ........................................................................................................... 1-5

Rotor Selection ........................................................................................................ 1-6

Chapter 2 Before Use ...............................................................................................................................2-1

Before Setting up ..................................................................................................... 2-2

Transporting the Centrifuge .................................................................................... 2-2

Location .................................................................................................................. 2-2

Aligning the Centrifuge ........................................................................................... 2-3

Mains Connection ................................................................................................... 2-3

Storage ..................................................................................................................... 2-4

Chapter 3 Control Panel ..........................................................................................................................3-1

Control Panel .......................................................................................................... 3-2

Keys ......................................................................................................................... 3-2

Chapter 4 Operation .................................................................................................................................4-1

Switch on Centrifuge ............................................................................................... 4-2

Door Opening ......................................................................................................... 4-2

Close Door .............................................................................................................. 4-2

Rotor Installation ..................................................................................................... 4-2

Entering Parameters ................................................................................................. 4-4

Centrifugation ......................................................................................................... 4-8

Short-term Centrifugation ....................................................................................... 4-8

Removing the Rotor ................................................................................................ 4-9

Aerosol-tight Rotors ................................................................................................. 4-9

Audible Alarm ......................................................................................................... 4-9

Turning off the Centrifuge .................................................................................... 4-10

Chapter 5 Maintenance and Care .........................................................................................................5-1

Cleaning Intervals .................................................................................................... 5-2

Cleaning .................................................................................................................. 5-2

Disinfection ............................................................................................................. 5-4

Thermo Scientific Heraeus Fresco 17 / 21 i

Contents

Decontamination ..................................................................................................... 5-5

Autoclaving .............................................................................................................. 5-5

Service of Thermo Fisher Scientific .......................................................................... 5-6

Shipping and Deposing of Accessories ..................................................................... 5-6

Chapter 6 Troubleshooting ....................................................................................................................6-1

Mechanical Emergency Door Release ...................................................................... 6-2

Troubleshooting by User ......................................................................................... 6-3

When to contact Service Technician ........................................................................ 6-5

Chemical Compatibility Chart ...............................................................................A-1

Contact Information ................................................................................................B-1

ii Heraeus Fresco 17 / 21 Thermo Scientific

Preface

Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.

The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is forbidden to copy or pass on this information without explicit approval.

Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty.

Scope of Supply

Article number Quantity Check

Centrifuge Heraeus Fresco 17 / 21 1

Power Supply Cable 1

Rotor 1

20360104 Allen Wrench 1

50133376 CD with Instruction Manual 1

If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.

This symbol refers to general hazards. WARNING means that injuries or material damage or contamination could occur. CAUTION means that material damage could occur.

This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe.

This symbol refers to hot surfaces Observe the information contained in the instruction manual to keep yourself and your environment safe.

Thermo Scientific Heraeus Fresco 17 / 21 iii

---------- -

Remaining risk: Inproper use can cause damages, contamincation, and injuries with fatal consequences.

Intended Use

• This centrifuge is a laboratory product designed to separate components by generation of Relative

Centrifugal Force. It separates human samples (e.g. blood, urine and other body fluids) collected in appropriate containers, either alone or after addition of reagents or other additives.

• As general purpose centrifuge, it is designed to also run other containers filled with chemicals,

environmental samples and other non-human body samples.

• Together with the hematocrit rotor 75003473 the centrifuge can be used as an in-vitro diagnostics

device to determine the haematicrit value by using centrifugation. Apply DIN 58933 to determine the hematocit value.

• The centrifuge is to be used for separating materials of different density or particle size suspended

in a liquid.

Maximum sample density at maximum speed: 1.2

Accident Prevention

WARNING If a hazardious situation occurs, turn off the power supply to the centrifuge

and leave the area immediately.

WARNING In case of a rotor failure the centrifuge can be damanged. The cooling agent can escape. Ventilate the room well and leave it. Inform Customer Service.

Prerequisite for the safe operation of the Heraeus Fresco 17 / 21 is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user.

The safety regulations contain the following basic recommendations:

• Maintain a radius of at least 30 cm around the centrifuge.

• Implementation of special measures which ensure that no one can approach the

centrifuge for longer than absolutely necessary while it is running.

• The mains plug must be freely accessible at all times. Pull out the power supply plug or

disconnect the power supply in an emergency.

iv Heraeus Fresco 17 / 21 Thermo Scientific

Precautions

In order to ensure safe operation of the Heraeus Fresco 17 / 21, the following general safety regulations must be followed:

• The centrifuge should be operated by trained specialists only.

• The centrifuge is to be used for its intended use only.

• While handling centrifuge, rotor, and samples you must wear laboratory clothing (e.g.

gloves).

• Plug the centrifuge only into sockets which have been properly grounded.

• Do not move the centrifuge while it is running.

• Do not lean on the centrifuge.

• Use only rotors and accessories for this centrifuge which have been approved by Thermo

Fisher Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the RCF value of the rotor.

• Do not use rotors which show any signs of corrosion and/or cracks.

• Do not touch the mechanical components of the rotor and do not make any changes to

the mechanical components.

• Use only with rotors which have been properly installed. Follow the instructions in

section “Rotor Installation” on page 4-2.

• Use only with rotors which have been loaded properly. Follow the instructions given in

the rotor manual.

• Never overload the rotor. Follow the instructions given in the rotor manual.

• Never open the lid until the rotor has come to a complete stop and this has been

confirmed in the display.

• The lid emergency release may be used in emergencies only to recover the samples from

the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door

Release” on page 6-2).

• Never use the centrifuge if parts of its cover panels are damaged or missing.

• Do not touch the electronic components of the centrifuge or alter any electronic or

mechanical components.

• Please observe the safety instructions.

Thermo Scientific Heraeus Fresco 17 / 21 v

Remaining risk: Inproper use can cause damages, contamincation, and injuries with fatal consequences.

Precautions

Please pay particular attention to the following aspects:

• Location: well-ventilated environment, set-up on a level and rigid surface with adequate

load-bearing capacity.

• Rotor installation:Check that the rotor is locked properly into place before operating the

centrifuge.

• Especially when working with corrosive samples (salt solutions, acids, bases), the

accessory parts and rotor chamber have to be cleaned carefully.

• Always balance the samples.

Centrifuging hazardous substances:

• Do not centrifuge explosive or flammable materials or substances which could react

violently with one another.

• The centrifuge is neither inert nor protected against explosion. Never use the centrifuge

in an explosion-prone environment.

• Do not centrifuge inflammable substances.

• Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms

without suitable safety precautions.

When centrifuging microbiological samples from the Risk Group II (according to the Biosafety Manual" of the World Health Organization (WHO)), aerosol-tight biological seals have to be used.

For materials in a higher risk group, extra safety measures have to be taken.

• If toxins or pathogenic substances have gotten into the centrifuge or its parts,

appropriate disinfection measures have to be taken (see “Disinfection” on page 5-4).

• Highly corrosive substances which can cause material damage and impair the

mechanical stability of the rotor, should only be centrifuged in corresponding protective tubes.

vi Heraeus Fresco 17 / 21 Thermo Scientific

Introduction and Description

Contents

• “Characteristics of the Heraeus Fresco 17 / 21” on page 1-2

• “Technical Data” on page 1-3

• “Directives, Standards and Guidelines” on page 1-4

• “Functions and Features” on page 1-5

• “Mains Supply” on page 1-5

• “Rotor Selection” on page 1-6

Thermo Scientific Heraeus Fresco 17 / 21 1-1

Introduction and Description

Characteristics of the Heraeus Fresco 17 / 21

You can use various rotors with commercially available containers.

The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and low-vibration operation even at high speeds, and guarantees a very long lifetime.

The user-friendly control panel makes it easy to pre-set the speed, RCF value, running time, and temperature. You can choose between the display of speed and RCF or the entry mode.

These settings can be changed even while the centrifuge is running.

With the help of the key you can also centrifuge a sample for just a few seconds, if called for.

The Heraeus Fresco 17 / 21 is equipped with various safety features:

• The housing is made of high-impact resistant plastic and the interior of armour steel.

• The lid is equipped with a view port and a lock.

• The lid of the centrifuge can only be opened while the centrifuge is switched on and the rotor has

come to a complete stop. The centrifuge cannot be started until the lid has been closed properly.

• An electronic imbalance recognition in ventilated centrifuges.

• For emergencies only, e.g. during power failures (see “Mechanical Emergency Door Release” on

page 6-2)

1-2 Heraeus Fresco 17 / 21 Thermo Scientific

Technical Data

The technical data of the Heraeus Fresco 17 / 21 is listed in the following table.

Table 1- 1. Technical Data Heraeus Fresco 17

Feature Value

Environmental Conditions -Use in interior Spaces

Introduction and Description

Technical Data

-Altitudes of up to 2,000 m above Sea Level

-Max. relative Humidity 80% up to 31°C; Decreasing linearly up to 50% relative Humidity at 40°C

Permissible Ambient Temperature during

+5°C bis +40°C +5°C bis +40°C +5°C bis +40°C

Operation

Permissible Ambient Temperature during

+2°C bis +50°C +2°C bis +50°C +2°C bis +50°C

Storage and Shipping

Overvoltage Category II II II

Pollution Degree 2 2 2

Heat Dissipation Heraeus Fresco 17 Heraeus Fresco 17 Heraeus Fresco 17

230 V 120 V 100 V

614 BTU/h 614 BTU/h 580 BTU/h

IP 20 20 20

Run Time unlimited unlimited unlimited

Max Speed n

Min Speed n

max

min

Maximum RCF Value at n

max

13 300 rpm (Depending on Rotor)

300 rpm 300 rpm 300 rpm

17 000 x g 17 000 x g 17 000 x g

Maximum Kinetic Energy <1,90 kNm <1,90 kNm <1,90 kNm

Noise Level at maximum Speed < 56 dB (A) < 56 dB (A) < 56 dB (A)

Dimensions

Hight Width Depth

230 mm 240 mm 350 mm

Weight without Rotor 10.5 kg 10.5 kg 10.5 kg

Table 1- 2. Technical Data Heraeus Fresco 21

Feature Value

Environmental Conditions -Use in interior Spaces

-Altitudes of up to 2,000 m above Sea Level

-Max. relative Humidity 80% up to 31°C; Decreasing linearly up to 50% relative Humidity at 40°C

Permissible Ambient Temperature during Operation

Permissible Ambient Temperature during Storage and Shipping

Thermo Scientific Heraeus Fresco 17 / 21 1-3

+5°C bis +40°C +5°C bis +40°C +5°C bis +40°C

+2°C bis +50°C +2°C bis +50°C +2°C bis +50°C

Introduction and Description

Directives, Standards and Guidelines

Table 1-2. Technical Data Heraeus Fresco 21

Feature Value

Overvoltage Category II II II

Pollution Degree 2 2 2

Heat Dissipation Heraeus Fresco 21 Heraeus Fresco 21 Heraeus Fresco 21

IP 20 20 20

Run Time unlimited unlimited unlimited

230 V 120 V 100 V

785 BTU/h 751 BTU/h 785 BTU/h

Max Speed n

max

14 800 rpm (Depending on Rotor)

Min Speed n

min

Maximum RCF Value at n

max

300 rpm 300 rpm 300 rpm

21 100 x g 21 100 x g 21 100 x g

Maximum Kinetic Energy <2,35 kNm <2,35 kNm <2,35 kNm

Noise Level at maximum Speed < 50 dB (A) < 50 dB (A) < 50 dB (A)

Dimensions

Hight Width Depth

230 mm 240 mm 350 mm

Weight without Rotor 10.5 kg 10.5 kg 10.5 kg

Directives, Standards and Guidelines

Table 1-3. Directives, Standards and Guidelines

Tension / Frequency

230V 50Hz/60Hz,

Marked

EC:

98/79/EC In-vitro-Diagnostica (IvD)

2006/42/EC Machine Directive

2004/108/EC EMC Directive

2006/95/EC Low Voltage Directive

100V 50Hz cCSAus: UL Std. No. UL 61010-1, 2

Produced and inspected according to the following standards and guidelines

EN 61010-1, 2

EN 61010-2-020, 2

Edition

EN 61010-2-101

EN 61326-1

EN 61326-2-6

EN 55011B

EN 61000-6-2

EN ISO 13485

CAN/CSA-C22.2 No. 61010-1-04

CAN/CSA-C22.2 No. 61010-2-020-09 Part 2-020:

IEC 61010-2-020 2

Edition

(Pollution degree 2, Overvoltage category II)

IEC 61010-2-101

14 800 rpm (Depending on Rotor)

230 mm 240 mm 350 mm

Edition

14 800 rpm (Depending on Rotor)

230 mm 240 mm 350 mm

1-4 Heraeus Fresco 17 / 21 Thermo Scientific

Introduction and Description

Functions and Features

The following table contains an overview of the electrical connection data for the Heraeus Fresco 17 /

21.

Table 1-4 . Functions and Features

Component / Function Description / Features

Structure / Housing Sheet steel chassis with attached plastic housing and steel

reinforcement

Rotor Chamber Plastic

Up to 48 ml of spilled liquids are retained in the chamber and cannot enter the instrument.

Drive Induction drive without carbon brushes

Keys and Display Easy-to-clean keypad and display surface

Controls Microprocessor-controlled

Internal Memory Most recent data is saved

Functions RCF-selection, temperature control, pretemp

Imbalance Recognition Electronic in ventilated centrifuges

Door Lock Automatic door closing and locking starting from an initial hold

position

Mains Supply

The following table contains an overview of the electrical connection data for the der Heraeus Fresco 17 / 21.This data is to be taken into consideration when selecting the mains connection socket.

Table 1-5 . Electrical connection data

Cat.

75002420 FRESCO 17 230 V 50 / 60 Hz 1.9A 330 W 4A, 2-pole circuit breaker 16 AT

75002421 FRESCO 17 120 V 60 Hz 2.2 A 330 W 8A, 2-pole circuit breaker 15 AT

75002422 FRESCO 17 100 V 60 Hz 2.4 A 330 W 8A, 2-pole circuit breaker 16 AT

75002425 Fresco 21 230 V 50 / 60 Hz 2.2 A 370 W 4A, 2-pole circuit breaker 16 AT

75002421 Fresco 26 120 V 60 Hz 4.4 A 370 W 8A, 2-pole circuit breaker 15 AT

75002422 Fresco 27 100 V 60 Hz 5.1 A 360 W 8A, 2-pole circuit breaker 16 AT

Mains voltage

Frequency

Rated current

Power consumption

Equipment fuse Building fuse

Thermo Scientific Heraeus Fresco 17 / 21 1-5

Introduction and Description

Rotor Selection

The Heraeus Fresco 17 / 21 is supplied with a rotor

Various rotors are available to choose from.

Microliter 24 x 2 7500 3424

Microliter 36 x 0.5 7500 3436

Dual Rotor 18x2 / 0.5 ml 7500 3418

PCR Rotor 4x8 7500 3440

PCR Rotor 8x8 7500 3489

Hematocrit Rotor 7500 3473

The technical data of the rotors and the corresponding adapters and reduction sleeves for various commercially available containers can be found in the corresponding rotor operating manuals.

For more information visit our website at: www.thermoscientific.com/centrifuge.

1-6 Heraeus Fresco 17 / 21 Thermo Scientific

Before Use

Contents

• “Before Setting up” on page 2-2

• “Transporting the Centrifuge” on page 2-2

• “Location” on page 2-2

• “Aligning the Centrifuge” on page 2-3

• “Mains Connection” on page 2-3

• “Storage” on page 2-4

Thermo Scientific Heraeus Fresco 17 / 21 2-1

+ 37 hidden pages

Thermo Heraeus Fresco 17, Heraeus Fresco 21 User manual

Specifications and Main Features

Frequently Asked Questions

User Manual