Thermo Hematocrit Rotor User manual

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Thermo Scientific

Hematocrit Rotor

For use with Thermo Scientific Heraeus Megafuge 8,

Sorvall ST 8 and SL 8 Centrifuges

Instruction Manual

50139071-a February 2013

Visit us online to register your warranty. www.thermoscientific.com/labwarranty

HERAEUS is a registered trademark of Heraeus Holding GmbH licensed to Thermo Fisher Scientific. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd. Hypaque is a registered trademark of Amersham Health As. RULON A and Tygon are registered trademarks of Saint-Gobain Performance Plastics. Alconox is a registered trademark of Alconox. Ficoll is a registered trademark of GE Healthcare. Haemo-Sol is a registered trademark of Haemo-Sol. Triton X-100 is a registered trademark of Sigma-Aldrich Co. LLC

All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

Manufacturer: Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Germany

The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or errorfree and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.

Original instruction manual 50139071-a printed in February 2013.

WEEE Compliance

This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product. See

www.thermo.com/rohsweee

Directives and the recyclers in your country.

for further information on Thermo Fisher Scientific’s compliance with these

Contents

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Items Supplied . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Rotor Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Thermo Scientific Auto-Lock Rotor Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Thermo Scientific Heraeus Megafuge 8 Centrifuge . . . . . . . . . . . . . . . . . . . . . . 10

Thermo Scientific Sorvall ST 8 Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Thermo Scientific SL 8 Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Rotor Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Removing the Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Rotor Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Before a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Filling the Hematocrit Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Rotor Temperature Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Rotor Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Maximum Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Using the Reading Graph . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Rotor Life-time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Maintenance and Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Cleaning Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Replace Sealing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

How To Deal With Broken Hematocrit Capillary Tubes . . . . . . . . . . . . . . . . . 29

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Thermo Fisher Scientific Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Thermo Scientific Hematocrit Rotor 5

Contents

RCF-Values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Chemical Compatibility Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

6 Hematocrit Rotor Thermo Scientific

Preface

Before starting to use the rotor, read through these instruction manual carefully and follow the instructions.

Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the seller’s warranty.

Items Supplied

Hematocrit Rotor 1

Anti-corrosion Oil 1

Linear scale 1

Quantity Check

Preface

Instruction manual 1

If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.

Intended Use

This rotor is used in combination with the according centrifuge as a laboratory product designed to separate components by generation of Relative Centrifugal Force. It separates human samples (e.g. blood, urine and other body fluids) collected in appropriate containers, either alone or after addition of reagents or other additives.

The rotor in the centrifuge is designed to run other containers filled with chemicals, environmental samples and other non-human body samples.

If the rotor is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Together with this hematocrit rotor the centrifuge becomes an in-vitro diagnostics device to define the hematocrit value by centrifugation.

Apply DIN 58933 to define the hematocrit value. To obtain reproducible results, standardization is required both in interest of patients and blood donors and to guarantee the comparability of research results. The hematocrit value allows you to calculate the rate of erythrocytes in the blood. This applies only if the Hematocrit Rotor is used with approved accessories and items listed in the items supplied.

This rotor should be operated by trained specialists only.

Thermo Scientific Hematocrit Rotor 7

---------- -

Precautions

In order to ensure safe operation of the Hematocrit Rotor, the following general safety regulations must be followed:

• Do not remove the magnet at the rotor bottom

• Do not use rotors which show any signs of corrosion and/or cracks. Do not touch the electronic

• Use only with rotors which have been loaded properly.

• Never overload the rotor.

• Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this

• Observe the safety instructions.

components of the rotor and do not make any changes to the electronic or mechanical components.

rule are commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the RCF value of the rotor.

Pay particular attention to the following aspects:

• Rotor installation: Check that the rotor is locked properly into place before operating the centrifuge.

• Always balance the samples.

Maximum sample density at maximum speed: 1,2

This symbol refers to general hazards. CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur.

This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe.

8 Hematocrit Rotor Thermo Scientific

Rotor Specifications

Contents

• “Thermo Scientific Heraeus Megafuge 8 Centrifuge” on page 10

• “Thermo Scientific Sorvall ST 8 Centrifuge” on page 11

• “Thermo Scientific SL 8 Centrifuge” on page 13

Rotor Specifications

Thermo Scientific Hematocrit Rotor 9

Rotor Specifications

Thermo Scientific Heraeus Megafuge 8 Centrifuge

Table 1. Heraeus 230V 50Hz/60Hz

Centrifuge Heraeus® Megafuge® 8

Catalog # 75007211

Weight empty [kg] 1.0

Maximum permissible load [g] 24 x 0.2

Maximum speed n

Maximum RCF value at n

[rpm] 13300

max

16810

max

Max. / min. radius [cm] 8.5 / 2.0

Pitch angle [°] 90

Accel. / braking time [s] 14 / 24

Sample heating at n temperature of 23 °C, running time 60 minutes

Aerosol-tight

[° C] referred to ambient

max

Permissible temperature range autoclavable °C 134

Tested by HPA, Porton-down, UK

Table 2. Heraeus 120V 60Hz

Centrifuge Heraeus® Megafuge® 8

Catalog # 75007210

Weight empty [kg] 1.0

Maximum permissible load [g] 24 x 0.2

Maximum speed n

Maximum RCF value at n

[rpm] 13300

max

16810

max

Max. / min. radius [cm] 8.5 / 2.0

Pitch angle [°] 90

Accel. / braking time [s] 14 / 24

Sample heating at n temperature of 23 °C, running time 60 minutes

Aerosol-tight

[° C] referred to ambient

max

Permissible temperature range autoclavable °C 134

Tested by HPA, Porton-down, UK

10 Hematocrit Rotor Thermo Scientific

Thermo Scientific Sorvall ST 8 Centrifuge

Table 3. Sorvall 230V 50/60Hz

Centrifuge Sorvall® ST 8

Catalog # 75007201

Weight empty [kg] 1.0

Maximum permissible load [g] 24 x 0.2

Rotor Specifications

Thermo Scientific Sorvall ST 8 Centrifuge

Maximum speed n

Maximum RCF value at n

[rpm] 13300

max

16810

max

Max. / min. radius [cm] 8.5 / 2.0

Pitch angle [°] 90

Accel. / braking time [s] 14 / 24

Sample heating at n temperature of 23 °C, running time 60 minutes

Aerosol-tight

[° C] referred to ambient

max

Permissible temperature range autoclavable °C 134

Tested by HPA, Porton-down, UK

Table 4. Sorvall 120V 60Hz

Centrifuge Sorvall® ST 8

Catalog # 75007200

Weight empty [kg] 1.0

Maximum permissible load [g] 24 x 0.2

Maximum speed n

Maximum RCF value at n

[rpm] 13300

max

16810

max

Max. / min. radius [cm] 8.5 / 2.0

Pitch angle [°] 90

Accel. / braking time [s] 14 / 24

Sample heating at n temperature of 23 °C, running time 60 minutes

Aerosol-tight

[° C] referred to ambient

max

Permissible temperature range autoclavable °C 134

Tested by HPA, Porton-down, UK

Thermo Scientific Hematocrit Rotor 11

Rotor Specifications

Thermo Scientific Sorvall ST 8 Centrifuge

Table 5. Sorvall 100V 50/60Hz

Centrifuge Sorvall® ST 8

Catalog # 75007202

Weight empty [kg] 1.0

Maximum permissible load [g] 24 x 0.2

Maximum speed n

Maximum RCF value at n

[rpm] 13300

max

16810

max

Max. / min. radius [cm] 8.5 / 2.0

Pitch angle [°] 90

Accel. / braking time [s] 14 / 24

Sample heating at n temperature of 23 °C, running time 60 minutes

Aerosol-tight

[° C] referred to ambient

max

Permissible temperature range autoclavable °C 134

Tested by HPA, Porton-down, UK

12 Hematocrit Rotor Thermo Scientific

Thermo Scientific SL 8 Centrifuge

Table 6. Thermo Scientific 230V 50/60Hz

Centrifuge SL 8

Catalog # 75007221

Weight empty [kg] 1.0

Maximum permissible load [g] 24 x 0.2

Rotor Specifications

Thermo Scientific SL 8 Centrifuge

Maximum speed n

Maximum RCF value at n

[rpm] 13300

max

16810

max

Max. / min. radius [cm] 8.5 / 2.0

Pitch angle [°] 90

Accel. / braking time [s] 14 / 24

Sample heating at n temperature of 23 °C, running time 60 minutes

Aerosol-tight

[° C] referred to ambient

max

Permissible temperature range autoclavable °C 134

Tested by HPA, Porton-down, UK

Table 7. Thermo Scientific120V 60Hz

Centrifuge SL 8

Catalog # 75007220

Weight empty [kg] 1.0

Maximum permissible load [g] 24 x 0.2

Maximum speed n

Maximum RCF value at n

Max. / min. radius [cm] 8.5 / 2.0

[rpm] 13300

max

16810

max

Pitch angle [°] 90

Accel. / braking time [s] 14 / 24

Sample heating at n temperature of 23 °C, running time 60 minutes

Aerosol-tight

[° C] referred to ambient

max

Permissible temperature range autoclavable °C 134

Tested by HPA, Porton-down, UK

Thermo Scientific Hematocrit Rotor 13

Accessories

Article No.

Capillaries 76000923

Sealing Kit 75000964

Rotor Seal Replacement 75003437

Replacement Linear Scale Reading Graph 76000938

Thermo Scientific Hematocrit Rotor 15

Thermo Scientific Auto-Lock Rotor Exchange

Contents

• “Rotor Installation” on page 18

• “Removing the Rotor” on page 19

Thermo Scientific Hematocrit Rotor 17

O-ring

Auto-Lock

Thermo Scientific Auto-Lock Rotor Exchange

Rotor Installation

CAUTION Unapproved or incorrectly combined accessories can cause serious damage to

the centrifuge.

This rotor is equipped with an Auto-Lock™-system.

This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have to be bolted onto the centrifuge spindle.

Proceed as follows:

1. Open the lid of the centrifuge and if necessary remove any dust, foreign objects or residue from the chamber. Auto-Lock

and O-ring must be clean and undamaged.

Figure 1. Auto-Lock

2. Hold the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle. The rotor clicks automatically into place.

CAUTION Do not force the rotor onto the centrifuge spindle. If the rotor is very light, then it may be necessary to press it onto the centrifuge spindle with a small amount of pressure.

3. Check if the rotor is properly installed by lifting it slightly on the handle. If the rotor can be pulled up, then it must be reclamped to the centrifuge spindle.

WARNING If the rotor cannot be properly locked in place after several attempts, then the Auto-Lock is defective and you are not permitted to operate the rotor. Check for any damage to the rotor. Damaged rotors must not be used. Keep the hub area clear of objects.

CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it at its handle.

18 Hematocrit Rotor Thermo Scientific

WARNING Be sure to check all seals before starting any aerosol-tight applications.

4. Close the centrifuge door.

Removing the Rotor

To remove the rotor, proceed as follows:

1. Open the centrifuge door.

2. Grab the rotor handle with both hands and press against the green Auto-Lock button. At the same time, pull the rotor directly upwards with both hands and remove it from the centrifuge spindle. Make sure not to jam the rotor while doing this.

Thermo Scientific Auto-Lock Rotor Exchange

Removing the Rotor

Figure 2. Handling of Auto-Lock

Thermo Scientific Hematocrit Rotor 19

Thermo Scientific Auto-Lock Rotor Exchange

Removing the Rotor

20 Hematocrit Rotor Thermo Scientific

Rotor Loading

Contents

• “Before a Run” on page 22

• “Filling the Hematocrit Capillaries” on page 22

• “Rotor Temperature Range” on page 22

• “Duration” on page 23

• “Rotor Loading” on page 23

• “Maximum Loading” on page 24

• “Using the Reading Graph” on page 24

Rotor Loading

• “Rotor Life-time” on page 25

Thermo Scientific Hematocrit Rotor 21

Rotor Loading

Before a Run

1. Please read and observe the safety instructions contained in these operating instructions and in the instructions for use.

2. Check the rotor and all accessory parts for damages such as cracks, scratches or traces of corrosion.

3. Check the rotor chamber, the centrifuge spindle and the Auto-Lock.

4. Check the rotor suitability using the “Chemical Compatibility Chart” on page 39.

Filling the Hematocrit Capillaries

Note Mind the lifetime of the capillaries.

1. Fill the hematocrit capillary (760000923) with a blood column of approximately 65 mm. Make sure that the second opening remains dry.

2. Close the dry end of the hematocrit capillary with sealing putty (75000964). To do this, push the hematocrit capillary vertically into the sealing putty until its rim touches the sealing putty’s plate.

Tilt it slightly and pull the hematocrit capillary carefully out of the putty. Make sure that the capillaries are well sealed with the sealing putty.

WARNING The rotor cannot be used for aerosol-tight applications. The O-ring on the rotor lock has the function to secure the rotor lid when screwed on. The external lid lip cannot be sealed.

Rotor Temperature Range

CAUTION Operate the rotor in a temperature range between -9°C and +40°C only. A

pre-tempering in a freezer below -9°C is not allowed.

Note The rotor can warm up in ventilated centrifuges. Temperature above 45°C the samples can be damaged. Let the rotor cool down between two runs.

22 Hematocrit Rotor Thermo Scientific

Duration

RCF 11 18,

1000

-------------



r××=

Rotor Loading

Duration

The duration depends on the RCF-value. For a clear separation the run should last at least 5 minutes. The following values are suggested for reproducible results.

Speed rpm Duration in Minutes

13300 8

Explanation of RCF-Value

The relative centrifugal force (RCF) is given as a multiple of the force of gravity g. It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of the type of device. Only the centrifuging radius and the speed come into play in it:

r = centrifuging radius in cm

n = rotational speed in rpm

The maximum RCF value is related to the maximum radius of the tube opening.

Remember that this value is reduced depending on the tubes and adapters used.

This can be accounted for in the calculation above if required.

Rotor Loading

1. Place the hematocrit capillary with its closed end pointing outwards (towards the rim of the rotor) horizontally into the hematocrit rotor. The hematocrit capillary must touch the sealing (7500

3437). The sealing protects the sensible capillaries from the hard rotor rim. The capillaries can break during a run when the sealing is missing.

2. Close the rotor lid by turning it clockwise.

CAUTION Ensure that a balancing hematocrit capillary is placed opposite in order to avoid imbalance and possible damage. If not all places are filled with hematocrit capillaries, they must be placed with an opposite.

Thermo Scientific Hematocrit Rotor 23

CAUTION Damaged parts must be replaced immediately.

adm

max

Maximum permissible load

Effective load

------------------------- ------------------------------- --------------

Rotor Loading

Maximum Loading

The rotor can run at high speeds. The rotor design has sufficient reserve stability even when spinning at top speed.

The safety system of the centrifuge requires that you do not overload the rotor.

There are two options available for centrifuging samples whose weight, including adapter, exceeds the maximum permissible load:

• Reduce the fill level.

• Reduce the speed.

Use the table or the formula:

adm

max

Actual Load (g/cavity) RPM

0.2 13300

0.3 10859

0.4 9405

0.5 8412

0.6 7679

0.7 7109

0.8 6650

0.9 6270

1.0 5948

= admissible speed = maximum speed

Using the Reading Graph

The reading graph (76000938) is supplied with the rotor. To read the values, proceed as follows:

max

1. Remove the hematocrit capillaries one by one from the rotor.

Note Be careful not to shake up the sample while removing. If the samples have been shaken up during removal, they can be centrifuge again.

2. Place the bottom end of the hematocrit capillary on the zero line with the blood column and the upper end of the plasma column on the 100% line of the graph. The dividing line between erythrocytes and plasma indicates the percentage of packed cells.

Note Be careful to place the hematocrit capillary at a right angle to the zero line.

24 Hematocrit Rotor Thermo Scientific

Rotor Life-time

The rotor has no life-time restrictions. For safety reasons please bear the following in mind:

• UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories

to direct sunlight.

• If the rotor shows signs of decoloration, deformation, wear or imbalance it must be replaced.

Rotor Loading

Rotor Life-time

Thermo Scientific Hematocrit Rotor 25

Maintenance and Care

Contents

• “Cleaning Intervals” on page 28

• “Cleaning” on page 29

• “Disinfection” on page 31

• “Decontamination” on page 32

• “Au to cl av ing ” on page 33

• “Thermo Fisher Scientific Service” on page 33

Maintenance and Care

Thermo Scientific Hematocrit Rotor 27

Touching Ends

Maintenance and Care

Cleaning Intervals

For the sake of personal, environmental, and material protection, you have to clean and if necessary disinfect the centrifuge on a regular basis.

Maintenance Recommended Interval

Clean rotor chamber Daily or when polluted

Clean rotor Daily or when polluted

Accessories Daily or when polluted

CAUTION Refrain from using any other cleaning or decontamination procedure than those recommended here, if you are not entirely sure that the intended procedure is safe for the equipment. Use only approved cleansers. If in doubt, contact Thermo Fisher Scientific.

Replace Sealing

1. Remove the old sealing.

2. Form the new sealing into a ring without twisting it.

3. Put the touching ends into the rotor groove so that they are placed between two capillary slots.

Press the cushion ring completely into the groove, avoiding creases or waves.

28 Hematocrit Rotor Thermo Scientific

How To Deal With Broken Hematocrit Capillary Tubes

WARNING Infectious material can get into the centrifuge when a tube breaks or as a

result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions. Broken capillary tubes have sharp edges and are a risk of injury

1. Remove the lid of the Hematocrit Rotor carefully.

2. Remove the larger pieces of the capillary tubes with tweezers.

3. Remove the rotor.

4. Remove the sealing slowly and carefully with the tweezers.

5. Clean and disinfect the rotor as described below.

6. Place a new sealing.

Maintenance and Care

Cleaning

When cleaning the centrifuge:

• Use warm water with a neutral solvent.

• Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions or

scrubbing powder.

• Rinse the cavities out thoroughly.

• Use a soft brush without metal bristles to remove stubborn residue.

• Afterwards rinse with distilled water.

• Place the rotors on a plastic grate with their cavities pointing down.

• If drying boxes are used, the temperature must never exceed 50 °C, since higher temperatures

could damage the material and shorten the lifetime of the parts.

• Use only disinfectants with a pH of 6-8.

• Dry aluminum parts off with a soft cloth.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (part no.

70009824). Also treat the cavities with oil.

• Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing

down.

CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment.

Thermo Scientific Hematocrit Rotor 29

Maintenance and Care

Cleaning

Clean centrifuge and accessories as follows:

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

5. Remove the centrifuge tubes and adapters.

6. Use a neutral cleaning agent with a pH 6-8 for cleaning.

7. Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 °C.

8. Clean the housing of the centrifuge as needed.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (part no.

70009824). Also treat the cavities with oil.

• Tread the bolt of the swing out rotor with bolt grease (part no. 75003786).

CAUTION When cleaning, do not allow liquids, especially organic solvents, to get on the drive shaft, the bearings, the Auto-Lock or the locks. Organic solvents break down the grease in the motor bearing. The drive shaft could freeze up.

After some applications their might be ice in the rotor chamber. Let the ice melt and drain it off. Clean the rotor chamber as described above.

30 Hematocrit Rotor Thermo Scientific

Disinfection

Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation.

Use a sprayer whenever possible so that all surfaces are covered evenly.

The rotor chamber and the rotor should be treated preferably with a neutral disinfectant.

Maintenance and Care

Disinfection

WARNING Infectious material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions. In case of contamination, make sure that others are not put at risk. Decontaminate the affected parts immediately. Take other precautions if need be.

Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other disinfectants.

Disinfect the rotor and accessories as follows:

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

5. Remove the centrifuge tubes and adapters and dispose of them or disinfect them.

6. Treat the rotor and accessories according to the instructions for the disinfectant (spray or soak in solution). Adhere strictly to the given application times.

7. Be sure the disinfectant can drain off the rotor.

8. Rinse the rotor and rotor lid thoroughly with water and then rub down.

9. Dispose of the disinfectant according to the applicable guidelines.

10. Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 °C.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (part no.

70009824). Also treat the cavities with oil.

• Tread the bolt of the swing out rotor with bolt grease (part no. 75003786).

Thermo Scientific Hematocrit Rotor 31

Maintenance and Care

Decontamination

Decontaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation.

WARNING Radioactive material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all neccessary precautions. In case of contamination, make sure that others are not put at risk. Decontaminate the affected parts immediately. Take other precautions if needed.

For general radioactive decontamination use a solution of equal parts of 70% ethanol, 10% SDS and water.

Disinfect the rotor and accessories as follows:

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

5. Remove the centrifuge tubes and adapters and dispose of them or disinfect them.

6. Rinse the rotor first with ethanol and then with de-ionized water.

• Adhere strictly to the given application times.

7. Be sure the decontamination solution can drain off the rotor.

8. Rinse the rotor and accessories thoroughly with water.

9. Dispose of the decontamination solution according to the applicable guidelines.

10. Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 °C.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (part no.

70009824). Also treat the cavities with oil.

• Tread the bolt of the swing out rotor with bolt grease (part no. 75003786).

32 Hematocrit Rotor Thermo Scientific

Autoclaving

1. Before autoclaving clean rotor and accessories.

2. Place the rotor on a flat surface.

Clean the rotor before autoclaving and rinse it with distilled water. Remove all accessories (tubes, adapters) from the rotor. Place the rotor on a flat surface.

Note No chemical additives are permitted in the steam.

Maintenance and Care

• Rotors and adapter can be autoclaved at 121 °C.

• The maximum permissible autoclave cycle is 20 minutes at 121 °C.

CAUTION Never exceed the permitted temperature and duration when autoclaving. If the rotor shows signs of corrosion or wear, it must be replaced.

WARNING Dangerous materials through leaky seals. The O-ring of a sealed bucket will be damaged through heat exposure. Remove it before autoclaving a sealed bucket. Use a new O-ring.

Autoclaving

Thermo Fisher Scientific Service

Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an authorized service technician. The service technicians check the following:

• the electrical equipment

• the suitability of the set-up site

• the lid lock and the safety system

•the rotor

• the fixation of the rotor and the drive shaft

Thermo Fisher Scientific offers inspection and service contracts for this work.

Thermo Scientific Hematocrit Rotor 33

RCF-Values

Speed (rpm) R

300 2.0 8.5 2 9

400 2.0 8.5 4 15

500 2.0 8.5 6 24

600 2.0 8.5 8 34

700 2.0 8.5 11 47

800 2.0 8.5 14 61

900 2.0 8.5 18 77

1000 2.0 8.5 22 95

1100 2.0 8.5 27 115

1200 2.0 8.5 32 137

1300 2.0 8.5 38 161

1400 2.0 8.5 44 186

1500 2.0 8.5 50 214

1600 2.0 8.5 57 243

1700 2.0 8.5 65 275

1800 2.0 8.5 72 308

min

max

RCF R

min

RCF R

max

1900 2.0 8.5 81 343

2000 2.0 8.5 89 380

2100 2.0 8.5 99 419

2200 2.0 8.5 108 460

2300 2.0 8.5 118 503

2400 2.0 8.5 129 547

2500 2.0 8.5 140 594

2600 2.0 8.5 151 642

2700 2.0 8.5 163 693

2800 2.0 8.5 175 745

2900 2.0 8.5 188 799

3000 2.0 8.5 201 855

3100 2.0 8.5 215 913

3200 2.0 8.5 229 973

3300 2.0 8.5 244 1035

3400 2.0 8.5 258 1099

Thermo Scientific Hematocrit Rotor 35

RCF-Values

Speed (rpm) R

min

max

RCF R

min

RCF R

3500 2.0 8.5 274 1164

3600 2.0 8.5 290 1232

3700 2.0 8.5 306 1301

3800 2.0 8.5 323 1372

3900 2.0 8.5 340 1445

4000 2.0 8.5 358 1520

4100 2.0 8.5 376 1597

4200 2.0 8.5 394 1676

4300 2.0 8.5 413 1757

4400 2.0 8.5 433 1840

4500 2.0 8.5 453 1924

4600 2.0 8.5 473 2011

4700 2.0 8.5 494 2099

4800 2.0 8.5 515 2189

4900 2.0 8.5 537 2282

5000 2.0 8.5 559 2376

max

5100 2.0 8.5 582 2472

5200 2.0 8.5 605 2570

5300 2.0 8.5 628 2669

5400 2.0 8.5 652 2771

5500 2.0 8.5 676 2875

5600 2.0 8.5 701 2980

5700 2.0 8.5 726 3088

5800 2.0 8.5 752 3197

5900 2.0 8.5 778 3308

6000 2.0 8.5 805 3421

6100 2.0 8.5 832 3536

6200 2.0 8.5 860 3653

6300 2.0 8.5 887 3772

6400 2.0 8.5 916 3892

6500 2.0 8.5 945 4015

6600 2.0 8.5 974 4140

6700 2.0 8.5 1004 4266

6800 2.0 8.5 1034 4394

6900 2.0 8.5 1065 4524

7000 2.0 8.5 1096 4656

7100 2.0 8.5 1127 4790

36 Hematocrit Rotor Thermo Scientific

RCF-Values

Speed (rpm) R

min

max

RCF R

min

RCF R

7200 2.0 8.5 1159 4926

7300 2.0 8.5 1192 5064

7400 2.0 8.5 1224 5204

7500 2.0 8.5 1258 5345

7600 2.0 8.5 1292 5489

7700 2.0 8.5 1326 5634

7800 2.0 8.5 1360 5782

7900 2.0 8.5 1395 5931

8000 2.0 8.5 1431 6082

8100 2.0 8.5 1467 6235

8200 2.0 8.5 1503 6390

8300 2.0 8.5 1540 6547

8400 2.0 8.5 1578 6705

8500 2.0 8.5 1616 6866

8600 2.0 8.5 1654 7028

8700 2.0 8.5 1692 7193

max

8800 2.0 8.5 1732 7359

8900 2.0 8.5 1771 7527

9000 2.0 8.5 1811 7697

9100 2.0 8.5 1852 7869

9200 2.0 8.5 1893 8043

9300 2.0 8.5 1934 8219

9400 2.0 8.5 1976 8397

9500 2.0 8.5 2018 8576

9600 2.0 8.5 2061 8758

9700 2.0 8.5 2104 8941

9800 2.0 8.5 2147 9127

9900 2.0 8.5 2192 9314

10000 2.0 8.5 2236 9503

10100 2.0 8.5 2281 9694

10200 2.0 8.5 2326 9887

10300 2.0 8.5 2372 10082

10400 2.0 8.5 2418 10278

10500 2.0 8.5 2465 10477

10600 2.0 8.5 2512 10678

10700 2.0 8.5 2560 10880

10800 2.0 8.5 2608 11084

Thermo Scientific Hematocrit Rotor 37

RCF-Values

Speed (rpm) R

min

max

RCF R

min

RCF R

10900 2.0 8.5 2657 11291

11000 2.0 8.5 2706 11499

11100 2.0 8.5 2755 11709

11200 2.0 8.5 2805 11921

11300 2.0 8.5 2855 12134

11400 2.0 8.5 2906 12350

11500 2.0 8.5 2957 12568

11600 2.0 8.5 3009 12787

11700 2.0 8.5 3061 13009

11800 2.0 8.5 3113 13232

11900 2.0 8.5 3166 13457

12000 2.0 8.5 3220 13684

12100 2.0 8.5 3274 13913

12200 2.0 8.5 3328 14144

12300 2.0 8.5 3383 14377

12400 2.0 8.5 3438 14612

max

12500 2.0 8.5 3494 14848

12600 2.0 8.5 3550 15087

12700 2.0 8.5 3606 15327

12800 2.0 8.5 3663 15570

12900 2.0 8.5 3721 15814

13000 2.0 8.5 3779 16060

13100 2.0 8.5 3837 16308

13200 2.0 8.5 3896 16558

13300 2.0 8.5 3955 16810

38 Hematocrit Rotor Thermo Scientific

Chemical Compatibility Chart

CCCLEARCRIMP

Chemical Compatibility Chart

CHEMICAL

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINIUM

BUNA N

,CLEARCRIMP

, POLYCLEAR

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN®ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL®NYLON

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

, TEFLON

RULON A

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON®VITON

2-mercaptoethanol SSU- SMS- SUSSUSS- SSSSUSSSSSS

Acetaldehyde S - U U ---M-U---MU U U MM- MS U - S - U

Acetone MSUUSUMSSUUSUSUUUSSUUSMMSUU

Acetonitrile SSU- SMS- SSUSUMUU- SMUUSSSSUU

Alconox

UUS- SSS- SSSSSSMSSSSSSSSSSSU

Allyl Alcohol ---U--S----S-SSMSSS- MS- - S- -

Aluminum Chloride UUSSSSUSSSSMSSSS- SSSSSMUUSS

Formic Acid (100%) - S M U - - U ----U-SMUUSS-US-US-U

Ammonium Acetate SSU- SSS- SSSSSSSU- SSSSSSSSSS

Ammonium Carbonate MSUSSSSSSSSSSSUU- SSSSSSMSSS

Ammonium Hydroxide (10%)

UUSUSSMSSSSS- SUMSSSSSSSSSMS

Ammonium Hydroxide (28%)

Ammonium Hydroxide (conc.)

UUSUSUMSSSSSUSUMSSSSSSSSSMS

UUUUSUMS- S- SUSUUSSS- MSSSS- U

Ammonium Phosphate U- S- SSSSSSSS- SSM- SSSSSSMSSS

Ammonium Sulfate UMS- SSUSSSSSSSSS- SSSSSSUSSU

Amyl Alcohol S - M U - - S S - M - S - M SSSSM---U-S-M

Aniline S S U U S U S M S UUUUUUU- SMUUSSSSUS

Sodium Hydroxide (<1%)

Sodium Hydroxide (10%)

Thermo Scientific Hematocrit Rotor 39

U- MSSS- - SMSS- SMMSSSSSSMSS- U

U- MU- - U- MMSSUSUUSSSSSSMSS- U

Chemical Compatibility Chart

CCCLEARCRIMP

CHEMICAL

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINIUM

BUNA N

,CLEARCRIMP

, POLYCLEAR

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN®ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL®NYLON

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

, TEFLON

RULON A

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON®VITON

Barium Salts MUS- SSSSSSSSSSSM- SSSSSSMSSS

Benzene SSUUSUMUSUUSUUUMUMUUUSUUSUS

Benzyl Alcohol S- UU- - MM- M- SUUUUUUU- MSM- S- S

Boric Acid USSMSSUSSSSSSSSSUSSSSSSSSSS

Cesium Acetate M- S- SSS- SSSS- SS- - SSSSSSMSSS

Cesium Bromide MSS- SSS- SSSSSSS- - SSSSSSMSSS

Cesium Chloride MSSUSSS- SSSSSSS- - SSSSSSMSSS

Cesium Formate MSS- SSS- SSSSSSS- - SSSSSSMSSS

Cesium Iodide MSS- SSS- SSSSSSS- - SSSSSSMSSS

Cesium Sulfate MSS- SSS- SSSSSSS- - SSSSSSMSSS

Chloroform UUUUSSMUSUUMUMUUUMMUUSUUUMS

Chromic Acid (10%) U- UUSUU- SSSUSSMUMSSUMSMUSSS

Chromic Acid (50%) U-UU-UU---SUUSMUMSSUMS-UM-S

Cresol Mixture SSU---S-SUUUUUU- - UU- USSSSUS

Cyclohexane SSS- SSSUSUSSUUUMSMUMMSUMMUS

Deoxycholate SSS- SSS- SSSSSSS- - SSSSSSSSSS

Distilled Water SSSSSSSSSSSSSSSSSSSSSSSSSSS

Dextran MSSSSSS- SSSSSSSSSSSSSSSMSSS

Diethyl Ether SSUUSSSUSUUSUUUUUUUUUSSSSMU

Diethyl Ketone S- UU- - M- SU- S- MUUUMM- US- - SUU

Diethylpyrocarbonate SSU- SSS- SSUSUSU- - SSSMSSSSSS

Dimethylsulfoxide SSUUSSS- SUSSUSUU- SSUUSSSSUU

Dioxane MS U U S S MMS U U S U MU U - MMMU SSSSUU

Ferric Chloride UUS---MS-M-S-S---SS---MUS-S

Acetic Acid (Glacial) SSUUSSUMSUSUUUUUMSUMUSUUS- U

40 Hematocrit Rotor Thermo Scientific

CCCLEARCRIMP

Chemical Compatibility Chart

CHEMICAL

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINIUM

BUNA N

,CLEARCRIMP

, POLYCLEAR

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN®ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL®NYLON

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

, TEFLON

RULON A

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON®VITON

Acetic Acid (5%) SSMSSSMSSSSSMSSSSSSSMSSMSSM

Acetic Acid (60%) S S U U S S U - S MS U U MU S MS MS MS MU S MU

Ethyl Acetate MMUUSSMMSSUSUMUU- SSUUSMMSUU

Ethyl Alcohol (50%) SSSSSSMSSSSSUSUSSSSSSSSMSMU

Ethyl Alcohol (95%) SSSUSSMSSSSSUSU- SSSMSSSUSMU

Ethylene Dichloride S- UU- - SM- UUSUUUUUUU- USU- S - S

Ethylene Glycol SSSSSSSSSSSS- SUSSSSSSSSMSMS

Ethylene Oxide Vapor S- U- - U- - SU- S- SM- - SSSUSUSSSU

Ficoll-Hypaque

MSS- SSS- SSSS- SS- SSSSSSSMSSS

Hydrofluoric Acid (10%) UUUM- - U- - UUS- SMUSSSSMSUUU- -

Hydrofluoric Acid (50%) UUUU- - U- - UUUUSUUUSSMMSUUU- M

Hydrochloric Acid (conc.)

UUUU- UUM- UMUUMUUU- S- USUUU- -

Formaldehyde (40%) MMMSSSSMSSSSMSSSUSSMSSSMSMU

Glutaraldehyde SSSS- - S- SSSSSSS- - SSS- - SSS- -

Glycerol MSS- SSSSSSSSSSSS- SSSSSSSSSS

Guanidine Hydrochloride

Haemo-Sol

UUS- SSS- SSSSSSS- - SSSSSSUSSS

SSS---S-SSSSSSS- - SSSSSSSSSS

Hexane SSS- SSS- SSUSUMUSSUSSMSUSSUS

Isobutyl Alcohol - - MU- - SS- U- SUSSMSSS- SSS- S- S

Isopropyl Alcohol MMMU SSSSSUSSUSUMSSSSSSSMMMS

Iodoacetic Acid SSM- SSS- SMSSMSS- MSSSSSMSSMM

Potassium Bromide USS- SSS- SSSSSSSSSSS- SSSMSSS

Potassium Carbonate MUSSSSS- SSSSSSUSSSSSSSSSSSS

Potassium Chloride USS- SSSSSSSSSSS- SSSSSSSUSSS

Thermo Scientific Hematocrit Rotor 41

Chemical Compatibility Chart

CCCLEARCRIMP

CHEMICAL

Potassium Hydroxide (5%)

Potassium Hydroxide (conc.)

Potassium Permanganate

,CLEARCRIMP

, POLYCLEAR

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINIUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN®ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL®NYLON

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

UUSSSSM- SSSS- SUSSSSSSSMUMSU

UUMU- - M- MSS- UMUUUSM- MU- UU- U

SSS- SSS- SSSUSSSM- SMSUSSMSUS

POLYSULFONE

POLYVINYL CHLORIDE

, TEFLON

RULON A

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON®VITON

Calcium Chloride MUSSSSSSSSSSSSMS- SSSSSSMSSS

Calcium Hypochlorite M - U - S M M S - M - S - S M S - SSSMSMUS- S

Kerosene SSS- SSSUSMUSUMMS- MMMSSUSSUS

Sodium Chloride (10%) S- SSSSSS- - - SSSSS- SSSS- SSM- S

Sodium Chloride (sat'd) U-SUSSS----SSSSS- SS- S- SSM- S

Carbon Tetrachloride U U MS S U MU S U U S U MU S S MMS MMMMU S S

Aqua Regia U-UU--U-----UUUUUUU-----S-M

Solution 555 (20%) SSS---S-SSSSSSS- - SSS- SSSSSS

Magnesium Chloride MSS- SSSSSSSSSSSSSSSSSSSMSSS

Mercaptoacetic Acid U S U - S M S - S M S UUUU- SUUSMSUSSSS

Methyl Alcohol SSSUSSMSSSSSUSUMSSSSSSSMSMU

Methylene Chloride UUUUMSS USUUSUUUUUMUUUSSMUSU

Methyl Ethyl Ketone SSUUSSMSSUUSUSUUUSSUUSSSSUU

Metrizamide

MSS- SSS- SSSS- SS- - SSSSSSMSSS

Lactic Acid (100%) - - S ------MSU-SSSMSS- MSMSS- S

Lactic Acid (20%) --SS-----MSM-SSSSSSSMSMSS- S

N-Butyl Alcohol S- SU- - S- - SM- USMSSSSMMSM- S- S

N-Butyl Phthalate SSU- SSS- SUUSUUUM- UUSUSMMSUS

N, N-Dimethylformamide

42 Hematocrit Rotor Thermo Scientific

SSSUSMS- SSUSUSUU- SSUUSMSSSU

CCCLEARCRIMP

Chemical Compatibility Chart

CHEMICAL

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINIUM

BUNA N

,CLEARCRIMP

, POLYCLEAR

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN®ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL®NYLON

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

, TEFLON

RULON A

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON®VITON

Sodium Borate MSSSSSSSSSSUSSSS- SSSSSSMSSS

Sodium Bromide USS- SSS- SSSSSSSS- SSSSSSMSSS

Sodium Carbonate (2%) MUSSSSSSSSSSSSUSSSSSSSSSSSS

Sodium Dodecyl Sulfate SSS- SSS- SSSSSSS- SSSSSSSSSSS

Sodium Hypochlorite (5%)

UUMSSMUSSMSSSMSSSSMSSSMUSMS

Sodium Iodide MSS- SSS- SSSSSSS- - SSSSSSMSSS

Sodium Nitrate SSS- SSSSSSSSSSSS- SSSSSUSSSS

Sodium Sulfate USS- SSSSSSSSSSSSSSSSSSSMSSS

Sodium Sulfide S-SS---S---SSSUU--S---SSM-S

Sodium Sulfite SSS- SSSSMSSSSSSM- SSSSSSSSSS

Nickel Salts USSSSS- SSS- - SSSS- SSSSSSMSSS

Oils (Petroleum) SSS---SUSSSSUUMSMUUSSSUSSSS

Oils (Other) S-S---SMSSSSUSSSSUSSSS- SSMS

Oleic Acid S- USSSUUSUSSMSSSSSSSSSMUSMM

Oxalic Acid UUMSSSUSSSSSUSUSSSSSSSSUMSS

Perchloric Acid (10%) U-U-SUU-SMM--MUMSMM-MSU-S-S

Perchloric Acid (70%) UUU- - UU- SUMUUMUUUMMUMSUUSUS

Phenol (5%) U S U - S MM- S U MU U S U MS MS U U S U MMMS

Phenol (50%) US U- SUM- SUMUUUUUSUMUUSUUUMS

Phosphoric Acid (10%) UUMSSSUSSSSU- SSSSSSSSSUMUSS

Phosphoric Acid (conc.) UUMM- - US- MSUUMMSSSMSMSUMU- S

Physiologic Media (Serum, Urine)

MSSS- - S- SSSSSSSSSSSSSSSSSSS

Picric Acid SSU- SMSSSMSUSSSUSSSSUSUMSMS

Pyridine (50%) USUUSUU- USSUUMUU- USMUS SUUUU

Thermo Scientific Hematocrit Rotor 43

Chemical Compatibility Chart

CCCLEARCRIMP

CHEMICAL

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINIUM

BUNA N

,CLEARCRIMP

, POLYCLEAR

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN®ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL®NYLON

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

, TEFLON

RULON A

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON®VITON

Rubidium Bromide MSS- SSS- SSSSSSS- - SSSSSSMSSS

Rubidium Chloride MSS- SSS- SSSSSSS- - SSSSSSMSSS

Sucrose MSS- SSSSSSSSSSSSSSSSSSSSSSS

Sucrose, Alkaline MSS- SSS- SSSSSSUSSSSSSSSMSSS

Sulfosalicylic Acid UUSSSSS- SSSUSSS- SSS- SSSUSSS

Nitric Acid (10%) USUSSUU- SUSU- SSSSSSSSSMSSSS

Nitric Acid (50%) USUMSUU- SUSUUMMUMMMSSSUSSMS

Nitric Acid (95%) U- UU- UU- - UUUUMUUUUMUUSUSS- S

Hydrochloric Acid (10%) U U M SSSU- SSSUUSUSSSSSSSSUMSS

Hydrochloric Acid (50%) UUUUSUU- SMSUUMUUSSSSMSMUUMM

Sulfuric Acid (10%) MUUSSUU- SSMUSSSSSSSSSSUUUSS

Sulfuric Acid (50%) MUUUSUU- SSMUUS UUMSSSSSUUUMS

Sulfuric Acid (conc.) MUUU- UUM- - MUUSUUUMSUMSUUU- S

Stearic Acid S-S---SMSSSS- SSSSSSSSSMMSSS

Tetrahydrofuran SSUUSUUMSUUS UUU- MUUUUSUSSUU

Toluene SSUUSSMUSUUSUUUSUMUUUSUSUUM

Trichloroacetic Acid UUU- SSUMSUSUUSM- MSSUUSUUUMU

Trichloroethane S-U---MU-U-SUUUUUUUUUSU- S- S

Trichloroethylene --UU---U-U-SUUUUUUUUUSU- U- S

Trisodium Phosphate ---S--M------S--SSS--S--S-S

Tris Buffer (neutral pH) USSSSSS- SSSSSSSSSSSSSSSSSSS

Triton X-100

SSS- SSS- SSSSSSSSSSSSSSSSSSS

Urea S- USSSS----SSSMSSSS- SSSMS- S

Hydrogen Peroxide (10%)

44 Hematocrit Rotor Thermo Scientific

UUMSSUU- SSSUSSSMUSSSSSSMSUS

CCCLEARCRIMP

CHEMICAL

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINIUM

BUNA N

,CLEARCRIMP

, POLYCLEAR

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN®ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL®NYLON

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

, TEFLON

RULON A

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON®VITON

Hydrogen Peroxide (3%) SMSSS- S- SSSSSSSSMSSSSSSSSSS

Xylene SSUSSSMUSUUUUUUMUMUUUSUMSUS

Zinc Chloride UUSSSSUSSSSSSSSSSSSSSSSUSSS

Zinc Sulfate USS- SSSSSSSSSSSSSSSSSSSSSSS

Citric Acid (10%) MSSMSSMSSSSSSSSSMSSSSSSSSSS

Polyethyleneterephthalate

Key

S Satisfactory

M = Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed

involved, etc.; suggest testing under actual conditions of use.

U U = Unsatisfactory, not recommended.

No data available. Because no organized chemical resistance data exists for materials under the stress

of centrifugation, when in doubt we recommend pretesting sample lots. suggest testing, using sample to avoid loss of valuable material.

Chemical resistance data is included only as a guide to product use.

Thermo Scientific Hematocrit Rotor 45

Contact

Country Telephone number

United States / Canada +1 866 984 3766

Latin America +1 866 984 3766

Austria +43 1 801 40 0

Belgium +32 53 73 42 41

France +33 2 2803 2180

Contact

Germany

Italy +39 02 95059 552

Netherlands +31 76 579 55 55

Nordic / Baltic Countries / CIS +358 9 329 10200

Russia +7 812 703 42 15

Spain / Portugal +34 93 223 09 18

Switzerland +41 44 454 12 22

UK / Ireland +44 870 609 9203

China

India +91 22 6716 2200

Japan +81 45 453 9220

Other Asian Countries +852 2885 4613

Australia +61 39757 4300

New Zealand +64 9 980 6700

Countries not listed

0800 1 536 376 +49 6184 90 6000

+86 21 6865 4588 +86 10 8419 3588

+49 6184 90 6000 +33 2 2803 2180

Thermo Scientific Hematocrit Rotor 47

Index

Accessories 15 Autoclaving 33 Auto-Lock™ 17

Before a Run 22

Chemical Compatibility Chart 39 Cleaning 29 Cleaning Intervals 28

Decontamination 32

Filling the Hematocrit Capillaries 22

Heraeus 10 How to deal with broken Hematocrit Capillary Tubes 29

Rotor Installation 18 Rotor Life-time 25 Rotor Loading 21, 22 Rotor Specifications 9 Rotor Temperature Range 22

Sorvall 11

Thermo Fisher Scientific Service 33

Using the Reading Graph 24

WEEE compliance 3

Intended Use 7 Items Supplied 7

Maintenance and Care 27 Maximum Loading 24

Precautions 8 Preface 7

RCF-Values 35 Removing the Rotor 19 Replace Sealing 28

Thermo Scientific Hematocrit Rotor 49

Thermo Hematocrit Rotor User manual

Specifications and Main Features

Frequently Asked Questions

User Manual

WEEE Compliance

Preface

Items Supplied

Intended Use

Precautions

Rotor Specifications

Thermo Scientific Heraeus Megafuge 8 Centrifuge

Thermo Scientific Sorvall ST 8 Centrifuge

Thermo Scientific SL 8 Centrifuge

Accessories

Thermo Scientific Auto-Lock Rotor Exchange

Rotor Installation

Removing the Rotor

Rotor Loading

Before a Run

Filling the Hematocrit Capillaries

Rotor Temperature Range

Duration

Rotor Loading

Maximum Loading

Using the Reading Graph

Rotor Life-time

Maintenance and Care

Cleaning Intervals

Replace Sealing

How To Deal With Broken Hematocrit Capillary Tubes

Cleaning

Disinfection

Decontamination

Autoclaving

Thermo Fisher Scientific Service

RCF-Values

Chemical Compatibility Chart

Contact

Index