Thermo Hematocrit Rotor User manual
Thermo Scientific
Hematocrit Rotor
For use with Thermo Scientific Heraeus Megafuge 8,
Sorvall ST 8 and SL 8 Centrifuges
Instruction Manual
50139071-a February 2013
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Original instruction manual 50139071-a printed in February 2013.
WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment
(WEEE) Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European
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www.thermo.com/rohsweee
Directives and the recyclers in your country.
for further information on Thermo Fisher Scientific’s compliance with these
C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Items Supplied . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Rotor Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Thermo Scientific Auto-Lock Rotor Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Thermo Scientific Heraeus Megafuge 8 Centrifuge . . . . . . . . . . . . . . . . . . . . . . 10
Thermo Scientific Sorvall ST 8 Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Thermo Scientific SL 8 Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Rotor Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Removing the Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Rotor Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Before a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Filling the Hematocrit Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Rotor Temperature Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Rotor Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Maximum Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Using the Reading Graph . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Rotor Life-time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Maintenance and Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Cleaning Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Replace Sealing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
How To Deal With Broken Hematocrit Capillary Tubes . . . . . . . . . . . . . . . . . 29
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Thermo Fisher Scientific Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Thermo Scientific Hematocrit Rotor 5
Contents
RCF-Values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Chemical Compatibility Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6 Hematocrit Rotor Thermo Scientific
Preface
Before starting to use the rotor, read through these instruction manual carefully and follow the
instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the seller’s warranty.
Items Supplied
Hematocrit Rotor 1
Anti-corrosion Oil 1
Linear scale 1
Quantity Check
1
Preface
Instruction manual 1
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Intended Use
This rotor is used in combination with the according centrifuge as a laboratory product designed to
separate components by generation of Relative Centrifugal Force. It separates human samples (e.g.
blood, urine and other body fluids) collected in appropriate containers, either alone or after addition of
reagents or other additives.
The rotor in the centrifuge is designed to run other containers filled with chemicals, environmental
samples and other non-human body samples.
If the rotor is used in a manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.
Together with this hematocrit rotor the centrifuge becomes an in-vitro diagnostics device to define the
hematocrit value by centrifugation.
Apply DIN 58933 to define the hematocrit value. To obtain reproducible results, standardization is
required both in interest of patients and blood donors and to guarantee the comparability of research
results. The hematocrit value allows you to calculate the rate of erythrocytes in the blood. This applies
only if the Hematocrit Rotor is used with approved accessories and items listed in the items supplied.
This rotor should be operated by trained specialists only.
Thermo Scientific Hematocrit Rotor 7
1
g
cm
3
---------- -
Precautions
Precautions
In order to ensure safe operation of the Hematocrit Rotor, the following general safety regulations
must be followed:
• Do not remove the magnet at the rotor bottom
• Do not use rotors which show any signs of corrosion and/or cracks. Do not touch the electronic
• Use only with rotors which have been loaded properly.
• Never overload the rotor.
• Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this
• Observe the safety instructions.
components of the rotor and do not make any changes to the electronic or mechanical
components.
rule are commercially available glass or plastic centrifuge tubes, provided they have been approved
for the speed or the RCF value of the rotor.
Pay particular attention to the following aspects:
• Rotor installation: Check that the rotor is locked properly into place before operating the
centrifuge.
• Always balance the samples.
Maximum sample density at maximum speed: 1,2
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
8 Hematocrit Rotor Thermo Scientific
Rotor Specifications
Contents
• “Thermo Scientific Heraeus Megafuge 8 Centrifuge” on page 10
• “Thermo Scientific Sorvall ST 8 Centrifuge” on page 11
• “Thermo Scientific SL 8 Centrifuge” on page 13
2
Rotor Specifications
Thermo Scientific Hematocrit Rotor 9
2
Rotor Specifications
Thermo Scientific Heraeus Megafuge 8 Centrifuge
Thermo Scientific Heraeus Megafuge 8 Centrifuge
Table 1. Heraeus 230V 50Hz/60Hz
Centrifuge Heraeus® Megafuge® 8
Catalog # 75007211
Weight empty [kg] 1.0
Maximum permissible load [g] 24 x 0.2
Maximum speed n
Maximum RCF value at n
[rpm] 13300
max
16810
max
Max. / min. radius [cm] 8.5 / 2.0
Pitch angle [°] 90
Accel. / braking time [s] 14 / 24
Sample heating at n
temperature of 23 °C, running time 60 minutes
Aerosol-tight
1
[° C] referred to ambient
max
16
No
Permissible temperature range autoclavable °C 134
1
Tested by HPA, Porton-down, UK
Table 2. Heraeus 120V 60Hz
Centrifuge Heraeus® Megafuge® 8
Catalog # 75007210
Weight empty [kg] 1.0
Maximum permissible load [g] 24 x 0.2
Maximum speed n
Maximum RCF value at n
[rpm] 13300
max
16810
max
Max. / min. radius [cm] 8.5 / 2.0
Pitch angle [°] 90
Accel. / braking time [s] 14 / 24
Sample heating at n
temperature of 23 °C, running time 60 minutes
Aerosol-tight
1
[° C] referred to ambient
max
16
No
Permissible temperature range autoclavable °C 134
1
Tested by HPA, Porton-down, UK
10 Hematocrit Rotor Thermo Scientific
Thermo Scientific Sorvall ST 8 Centrifuge
Table 3. Sorvall 230V 50/60Hz
Centrifuge Sorvall® ST 8
Catalog # 75007201
Weight empty [kg] 1.0
Maximum permissible load [g] 24 x 0.2
2
Rotor Specifications
Thermo Scientific Sorvall ST 8 Centrifuge
Maximum speed n
Maximum RCF value at n
[rpm] 13300
max
16810
max
Max. / min. radius [cm] 8.5 / 2.0
Pitch angle [°] 90
Accel. / braking time [s] 14 / 24
Sample heating at n
temperature of 23 °C, running time 60 minutes
Aerosol-tight
1
[° C] referred to ambient
max
16
No
Permissible temperature range autoclavable °C 134
1
Tested by HPA, Porton-down, UK
Table 4. Sorvall 120V 60Hz
Centrifuge Sorvall® ST 8
Catalog # 75007200
Weight empty [kg] 1.0
Maximum permissible load [g] 24 x 0.2
Maximum speed n
Maximum RCF value at n
[rpm] 13300
max
16810
max
Max. / min. radius [cm] 8.5 / 2.0
Pitch angle [°] 90
Accel. / braking time [s] 14 / 24
Sample heating at n
temperature of 23 °C, running time 60 minutes
Aerosol-tight
1
[° C] referred to ambient
max
16
No
Permissible temperature range autoclavable °C 134
1
Tested by HPA, Porton-down, UK
Thermo Scientific Hematocrit Rotor 11
2
Rotor Specifications
Thermo Scientific Sorvall ST 8 Centrifuge
Table 5. Sorvall 100V 50/60Hz
Centrifuge Sorvall® ST 8
Catalog # 75007202
Weight empty [kg] 1.0
Maximum permissible load [g] 24 x 0.2
Maximum speed n
Maximum RCF value at n
[rpm] 13300
max
16810
max
Max. / min. radius [cm] 8.5 / 2.0
Pitch angle [°] 90
Accel. / braking time [s] 14 / 24
Sample heating at n
temperature of 23 °C, running time 60 minutes
Aerosol-tight
1
[° C] referred to ambient
max
16
No
Permissible temperature range autoclavable °C 134
1
Tested by HPA, Porton-down, UK
12 Hematocrit Rotor Thermo Scientific
Thermo Scientific SL 8 Centrifuge
Table 6. Thermo Scientific 230V 50/60Hz
Centrifuge SL 8
Catalog # 75007221
Weight empty [kg] 1.0
Maximum permissible load [g] 24 x 0.2
2
Rotor Specifications
Thermo Scientific SL 8 Centrifuge
Maximum speed n
Maximum RCF value at n
[rpm] 13300
max
16810
max
Max. / min. radius [cm] 8.5 / 2.0
Pitch angle [°] 90
Accel. / braking time [s] 14 / 24
Sample heating at n
temperature of 23 °C, running time 60 minutes
Aerosol-tight
1
[° C] referred to ambient
max
16
No
Permissible temperature range autoclavable °C 134
1
Tested by HPA, Porton-down, UK
Table 7. Thermo Scientific120V 60Hz
Centrifuge SL 8
Catalog # 75007220
Weight empty [kg] 1.0
Maximum permissible load [g] 24 x 0.2
Maximum speed n
Maximum RCF value at n
Max. / min. radius [cm] 8.5 / 2.0
[rpm] 13300
max
16810
max
Pitch angle [°] 90
Accel. / braking time [s] 14 / 24
Sample heating at n
temperature of 23 °C, running time 60 minutes
Aerosol-tight
1
[° C] referred to ambient
max
16
No
Permissible temperature range autoclavable °C 134
1
Tested by HPA, Porton-down, UK
Thermo Scientific Hematocrit Rotor 13
3
Accessories
Accessories
Article No.
Capillaries 76000923
Sealing Kit 75000964
Rotor Seal Replacement 75003437
Replacement Linear Scale Reading Graph 76000938
Thermo Scientific Hematocrit Rotor 15
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