Techne TC-3000X User Manual

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TC-3000 & TC-3000X

OPERATOR'S MANUAL

Issue 3

10/08

CONTENTS

PAGE INTRODUCTION 1 SAFETY AND INSTALLATION 2 SÉCURITÉ ET CONSIGNES D'INSTALLATION 5 SICHERHEITS- UND INSTALLATIONSINFORMATIONEN 8 INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONE 11 INFORMACIÓN DE SEGURIDAD E INSTALACIÓN 14 BEFORE SWITCHING ON 17 CONTACT INFORMATION 18 TC-3000 & TC-3000X SPECIFICATIONS 19 USER OPERATION AND CONSUMABLES 21

Heated lid 21 Tubes or reaction vessels 22

0.5ml tubes 23 Switching on 23

FRONT PANEL CONTROLS 24

The LCD display 24 Indicators 24 The keys 25

PROGRAMMING THE TC-3000 & TC-3000X 26

Programming Options 27 Option 1: Creating programs using templates 27 Option 2: Creating a new program 31 Example programs 32 Programming 32 To program increment or decrement temperature or time. 35 To run a program 39

INFORMATION 41 CONFIGURATION 41 RUNNING THE TC-3000 & TC-3000X FROM A PC 44

Connecting one unit to the computer using an RS 232 cable 44 Connecting several units to the computer using the daisy chain cables 45

NOTES ON TRANSFERRING PROTOCOLS FROM OTHER THERMAL CYCLERS 47 USER MAINTENANCE 48

Cleaning your TC-3000/X 48 Heater Block removal 48

SOME FREQUENTLY ASKED QUESTIONS 49 ADDITIONAL INFORMATION 50

Fault Finding 50 Fuses 50 The heated lid over-temperature cut-out 50 Insulation testing 50 Interchangeable blocks 50 Accessories 51 Replacement parts 52

INTRODUCTION

The TC-3000 and larger capacity TC-3000X provide the researcher with the means of accurately controlling the temperature profile of samples. It has many scientific applications, including PCR1and sequencing. Both units can cycle samples between 4°C and 99°C.

The TC-3000 and TC-3000X are programmed by means of an integral keypad and LCD display. A program, which can be recalled from memory, consists of:

• a series of specified temperatures in °C

• the times for which each specified temperature will be held (Hold Times)

• the desired heating or cooling rates, in °C/sec, between each specified temperature

• optional instructions as to whether the times and/or the temperatures are to increase or

decrease when a cycle is repeated.

The memory can store up to 80 programs.

Before using the thermal cycler, make sure you have read this manual carefully. If there is any doubt relating to the proper use of this equipment, the staff at Bibby Scientific Ltd or your supplier will be happy to assist you.

Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and

development and applied fields other than human in-vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC.

SAFETY AND INSTALLATION

OPERATOR SAFETY

Please read this manual carefully before using the thermal cycler. If the equipment is not used in the manner described in this manual the protection provided by the equipment might be impaired.

All Bibby Scientific Ltd instruments are designed to conform to international safety requirements and are fitted with an over-temperature cut-out. If a safety problem should be encountered then switch off the unit at the mains socket and remove the plug from the electricity supply.

WARNING

HIGH TEMPERATURES ARE DANGEROUS as they can cause serious burns to operators and ignite combustible material. Users should be aware of the following potential hazards:

• USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS

• DO NOT use combustible substances near hot objects

• DO NOT operate the instrument in the vicinity of inflammable liquids or gases

• DO NOT place any liquid directly into the instrument, always use the appropriate plastic consumables

After heating samples, remember that parts of the instrument, namely the lid and block and any associated accessories may be very hot.

UNPACKING

When unpacking please ensure that the following have been removed from the packaging:

• Thermal cycler

• Mains cable

• Guarantee card

• Decontamination certificate

The user is advised to keep the original packaging in case the instrument ever needs to be returned for service or repair. Bibby Scientific Ltd accepts no responsibility for damage incurred unless the unit is correctly packed and transported in its original packaging.

Please note that the screen of the thermal cycler is easily damaged by sharp objects such as pens, pencils and fingernails. This type of damage will be considered as misuse and invalidate the guarantee for this component.

INSTALLATION

The instrument should be carried using both hands. Never move or carry the instrument when in use or connected to the mains electricity supply.

1. All Bibby Scientific Ltd instruments are supplied with a power cable; this may be integral or plug-in.

2. Before connecting the instrument to the mains electricity supply, check the voltage against the rating plate (located on the back of the unit). Ensure that the voltage selector switch (located above

the On/Off switch) is set to the appropriate voltage for the local supply. Please note that the unit must be earthed to ensure proper electrical safety. (The TC-3000X has a switch-mode power supply which is suitable for use with universal AC input voltages between 90 and 260V. It is not necessary to change the fuses when using the TC-3000X). Connect the mains cable to a suitable plug according to the table below.

Connections 220V-240V, 50/60Hz Supply 110V-120V, 50/60Hz Supply

Live brown black Neutral blue white Earth green/yellow green

3. UK ONLY: The fused plug supplied with the mains cable is fitted with a 10 Amp fuse to protect the instrument and the user.

4. TC3000 units showing 230V, 50/60Hz on the rating plate also operate between 210 and 260V; units with 115V, 50/60Hz also operate between 110 and 130V and units marked with 100V will operate between 90 and 110V. In all cases the heating rate will degrade by approximately 8% at the extremes of the voltage range.

5. Plug the mains cable into the socket on the back of the instrument.

6. Place the unit on a suitable flat bench or in a fume cupboard if required, ensuring that the air inlet vents on the underside are free from obstruction. Position the instrument with a minimum distance all round of 200mm from walls or other items and between each unit if multiple units are being used to permit efficient air flow for each instrument.

7. Switch on the instrument:

I mains switch On

O mains switch Off

Replacement cable

Should the mains lead need replacement, a cable of 1mm

of harmonized code H05VV-F connected to

an IEC320 plug should be used. IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN.

WORKING CONDITIONS

The thermal cycler is designed operate under the following conditions:

• Indoor use

• Ambient temperature range +5°C to +40°C

• Altitude to 2000m

• Mains supply fluctuations not exceeding 10%

• Over voltage category II IEC 60364-4-443

• Pollution degree 2

Note: The control specifications are quoted at an ambient temperature of 20°C. The specification may deteriorate outside an ambient temperature of 10°C to 30°C.

The instrument has been tested for radio frequency interference and is certified under EN61326.

GUARANTEE

The instrument is guaranteed against any defect in material or workmanship for the period specified on the enclosed guarantee card. This period is effective from the date of purchase; within this period all defective parts will be replaced free of charge provided that the defect is not the result of an accident, misuse or negligence. The screen of the thermal cycler can be damaged by sharp objects such as pens, pencils and fingernails. Damage of this sort will be considered misuse and will invalidate the guarantee for this component.

Servicing under this guarantee should be obtained from the supplier of the instrument.

This manual has been prepared for the convenience of Bibby Scientific Ltd's customers and nothing in this manual shall be taken as a warranty, condition or representation concerning the description, merchantability, fitness for purpose or otherwise of the unit or components.

Notwithstanding the description and specification(s) of the instruments contained in the operator's manual, Bibby Scientific Ltd reserves the right to make such changes as it sees fit to the instruments or to any of the components.

SÉCURITÉ ET CONSIGNES D'INSTALLATION

SÉCURITÉ DE L’OPÉRATEUR

Lire attentivement le présent manuel avant d'utiliser le cycleur thermique. Si ce matériel n'est pas utilisé de la manière décrite dans le présent manuel, la protection fournie risque d'être compromise.

Tous les appareils Bibby Scientific Ltd sont conçus pour être conformes aux exigences internationales de sécurité et sont dotés d'une coupure en cas de surchauffe. Si un problème de sécurité survient, mettre l'appareil hors tension au niveau de la prise secteur et débrancher la fiche de l'alimentation électrique.

AVERTISSEMENT

Les TEMPÉRATURES ÉLEVÉES SONT DANGEREUSES car elles peuvent provoquer de graves brûlures chez l'opérateur et enflammer les matériaux combustibles. Les utilisateurs devront être conscients des dangers potentiels suivants :

• AGIR AVEC PRUDENCE ET PORTER DES GANTS DE PROTECTION POUR LES MAINS

• NE PAS utiliser de substances combustibles à proximité d'objets chauds

• NE PAS utiliser l'appareil au voisinage de liquides ou de gaz inflammables

• NE PAS placer un quelconque liquide directement sur l'appareil; toujours utiliser les consommables en plastique appropriés

Rappel : après le chauffage d'échantillons, certaines parties de l'appareil, notamment le couvercle et le bloc, ainsi que les accessoires associés, peuvent être brûlants.

DÉBALLAGE

Au moment du déballage, vérifier que l'emballage contenait les articles suivants :

• Cycleur thermique

• Câble secteur

• Carte de garantie

• Certificat de décontamination

Il est conseillé à l'utilisateur de conserver l'emballage d'origine s'il devait s'avérer nécessaire de renvoyer l'appareil pour une intervention d'entretien ou de réparation. Bibby Scientific Ltd n'accepte aucune responsabilité pour les dégâts survenus si l'appareil n'est pas correctement emballé et transporté dans sont emballage d'origine.

Noter que des objets pointus tels que crayons, stylos et ongles peuvent facilement endommager l'écran du cycleur thermique. Ce type de dégâts sera considéré comme une mauvaise utilisation et annulera la garantie de ce composant.

INSTALLATION

Porter l'appareil à deux mains. Ne jamais déplacer ou transporter l'appareil lorsqu'il est en service ou branché à l'alimentation électrique sur secteur.

1. Tous les appareils Bibby Scientific Ltd sont livrés avec un câble d'alimentation, qui peut être intégré à l'appareil ou muni d'une fiche.

2. Avant de relier l'instrument à l'alimentation générale électrique, veuillez vérifier la tension sur la plaque de contrôle (située à l'arrière de l'appareil). Assurez-vous que le sélecteur de tension (placé au-dessus du bouton "Marche/Arrêt") est réglé sur la tension appropriée pour l'alimentation locale. Veuillez noter que l'appareil doit être connecté à la terre pour assurer sa sécurité électrique. (Le TC-3000X a une alimentation électrique avec un sélecteur de tension qui vous permet un choix entre 90 et 260V : Il n'est pas nécessaire de changer les fusibles). Reliez le câble d'alimentation à une prise appropriée selon la table ci-dessous.

Connexions Alimentation 220V-240 V, 50/60 Hz Alimentation 110V-120 V, 50/60 Hz

Phase marron noir Neutre bleu blanc Te rre vert/jaune vert

3. ROYAUME-UNI SEULEMENT: La prise à fusible fournie avec le câble secteur est munie d'un fusible 10 A destiné à protéger l'appareil et l'utilisateur.

4. Les appareils portant l'indication 230 V, 50/60 Hz sur la plaque signalétique fonctionnent également entre 210 et 260 V; les appareils portant l'indication 120 V, 50/60 Hz fonctionnent également entre 100 et 130 V et les appareils portant l'indication 100 V fonctionneront entre 90 et 110 V. Dans tous les cas, la puissance de chauffage se dégrade d'environ 8 % aux extrêmes de la plage de tension.

5. Brancher le câble secteur dans la prise au dos de l'appareil.

6. Disposer l'appareil sur une paillasse ou sous une hotte aspirante si nécessaire, en veillant à ce que les prises d'air situées au-dessous ne soient pas obstruées. Positionner l'appareil avec tout autour un écartement minimum de 200 mm par rapport aux murs ou autres éléments, ainsi qu'entre chaque appareil en cas d'utilisation d'appareils multiples, afin de permettre une circulation d'air efficace pour chaque appareil.

7. Allumer l'appareil.

I Interrupteur secteur en position Marche

O Interrupteur secteur en position Arrêt

Câble de rechange

S'il s'avère nécessaire de remplacer le cordon secteur, utiliser un câble de 1 mm

au code harmonisé

H05VV-F raccordé à une fiche IEC320. EN CAS DE DOUTE, CONSULTER UN ÉLECTRICIEN QUALIFIÉ.

CONDITIONS DE TRAVAIL

Le cycleur thermique est conçu pour fonctionner dans les conditions suivantes:

• Utilisation en intérieur

• Plage de température ambiante : +5°C à +40°C

• Altitude jusqu'à 2000 m

• Fluctuations de l'alimentation sur secteur ne dépassant pas 10%

• Surtensions de catégorie II selon la norme IEC 60364-4-443

• Degré de pollution 2

Remarque: Les caractéristiques de régulation sont indiquées à une température ambiante de 20°C. Ces caractéristiques peuvent se détériorer en dehors d'une température ambiante de 10°C à 30°C.

L'appareil a été testé en matière de perturbations radioélectriques et est certifié selon la norme EN61326.

GARANTIE

L'appareil est garanti contre tout défaut de matériaux ou vice de fabrication pendant la période précisée sur la carte de garantie jointe. Cette période s'applique à compter de la date d'achat; pendant cette période, toutes les pièces défectueuses seront remplacées gratuitement à condition que le défaut ne soit pas le résultat d'un accident, d'une mauvaise utilisation ou d'une négligence. Des objets pointus tels que crayons, stylos et ongles peuvent endommager l'écran du cycleur thermique. Les dégâts de cette sorte seront considérés comme une mauvaise utilisation et annuleront la garantie de ce composant.

Dans le cadre de cette garantie, le service après-vente est à obtenir auprès du fournisseur de l'appareil.

Le présent manuel a été préparé pour le confort des clients de Bibby Scientific Ltd et rien dans son contenu ne doit être pris comme une garantie, une condition ou une affirmation concernant la description, la qualité marchande, l'adéquation à un usage particulier ou autre de l'appareil ou de ses composants.

Nonobstant la description et les caractéristiques techniques des appareils figurant dans le manuel de l'utilisateur, Bibby Scientific Ltd se réserve le droit d'apporter aux appareils ou à n'importe quel composant les changements jugés utiles.

SICHERHEITS - UND INSTALLATIONSINFORMATIONEN

SICHERHEIT DES BEDIENPERSONALS

Lesen Sie diese Anleitung vor Verwendung des Thermocyclers bitte sorgfältig durch. Wenn das Gerät nicht entsprechend der Bedienungsanleitung eingesetzt wird, können die Schutzfunktionen des Gerätes beeinträchtigt werden.

Alle Geräte von Bibby Scientific Ltd entsprechen den internationalen Sicherheitsanforderungen und sind mit einer Übertemperatur-Schutzvorrichtung ausgestattet. Bei einer Sicherheitsstörung bitte das Gerät an der Steckdose ausschalten und den Netzstecker ziehen.

ACHTUNG

HOHE TEMPERATUREN STELLEN EINE GEFAHRENQUELLE DAR. Sie können schwere Brandverletzung verursachen und brennbare Stoffe entzünden. Der Benutzer sollt sich mit den möglichen Gefahren vertraut machen:

• UMSICHTIG VORGEHEN UND SCHUTZHANDSCHUHE TRAGEN

• KEINE brennbaren Stoffe in der Nähe heißer Gegenstände verwenden

• Das Gerät NICHT in der Nähe entzündlicher Flüssigkeiten oder Gase betreiben

• Flüssigkeiten NICHT direkt auf das Gerät auftragen - verwenden Sie stets die entsprechenden Kunststoffeinsätze

Nach der Erhitzung von Proben daran denken, dass bestimmte Geräteteile wie Deckel, Thermoblock und Zubehörteile sehr heiß sein können.

AUSPACKEN

Beim Auspacken darauf achten, dass der folgende Lieferumfang vorhanden ist:

• Thermocycler

• Netzkabel

• Garantiekarte

• Dekontaminationszertifikat

Wir empfehlen die Originalverpackung aufzubewahren, falls das Gerät zwecks Wartung oder Reparatur zurückgeschickt werden muss. Bibby Scientific Ltd übernimmt keine Verantwortung für Transportschäden, wenn das Gerät nicht ordnungsgemäß verpackt in der Originalpackung verschickt wird.

Achten Sie bitte darauf, dass die Anzeige des Thermocyclers durch spitze bzw. scharfe Gegenstände wie Stifte, Bleistifte und Fingernägel leicht zerkratzt wird. Diese Art von Beschädigung gilt als Missbrauch des Geräts und führt zur Ungültigkeit der Garantie für dieses Gerät.

INBETRIEBNAHME

Das Gerät mit beiden Händen tragen. Das Gerät unter keinen Umständen transportieren, wenn es in Betrieb ist, oder während das Gerät noch am Netz angeschlossen ist.

1. Alle Geräte von Bibby Scientific Ltd werden mit einem Netzkabel geliefert, das entweder eingesteckt wird oder fest mit dem Gerät verbunden ist.

2. Überprüfen Sie die Spannungsangabe auf dem Typenschild (an der Rückseite des Gerätes), bevor Sie das Gerät am Spannungsnetz anschliessen. Stellen sie sicher, dass sich der Wahlschalter des Spannungsselektors (oberhalb des An/Ausschalters) in der richtigen Position befindet. Bitte beachten Sie, dass das Gerät aus Gründen der elektrischen Sicherheit geerdet sein muss. Der TC3000X hat einen Switch-Mode Power Supply, der mit universellen AC Eingangsspannungen zwischen 90 und 260V genutzt werden kann. Es ist nicht notwendig, dabei die Sicherungen des TC3000X zu wechseln. Schliessen Sie das Netzkabel an eine geeignete Steckdose an, entsprechend der unten aufgeführten Tabelle.

Anschluss 220/240V, 50/60Hz 110V/120V, 50/60Hz

Phase Braun Schwarz Neutral Blau Weiß Erde Grün/Gelb Grün

3. NUR FÜR GROSSBRITANNIEN: der mit dem Netzkabel gelieferte Sicherungsstecker enthält eine 10 Amp. Sicherung zum Schutz des Geräts und des Anwenders.

4. Geräte mit der Bezeichnung 230V, 50/60Hz auf dem Typenschild können mit Spannungen zwischen 210 und 260V betrieben werden; Geräte für 120V, 50/60Hz können mit Spannungen zwischen 100 und 130V betrieben werden. Bei Geräten für 100V gilt der Bereich 90 bis 110V. In allen Fällen reduziert sich die Erhitzungsrate um etwa 8% in den äußeren Spannungsbereichen.

5. Stecken Sie das Netzkabel in die Buchse auf der Geräterückseite ein.

6. Stellen Sie das Gerät auf einen geeigneten ebenen Tisch oder in einem Abzugsschrank auf und sorgen Sie dafür, dass die Lufteinlassschlitze auf der Geräteunterseite nicht blockiert sind. Das Gerät muss einen Mindestabstand von 200 mm zu Wänden und anderen Gegenständen bzw. zu anderen Geräten dieser Art aufweisen, um einen ausreichenden Luftstrom zu gewährleisten.

7. Schalten Sie das Gerät ein:

I Netzschalter Ein

O Netzschalter Aus

Ersatzkabel

Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1 mm2 Adernquerschnitt und Europastecker (IEC 320) benötigt. IM ZWEIFELSFALL EINEN ELEKTRO-

FACHMANN HINZUZIEHEN.

EINSATZBEDINGUNGEN

Der Thermocycler ist für den Einsatz unter folgenden Bedingungen ausgelegt:

• Gebrauch in Innenräumen

• Umgebungstemperatur zwischen +5°C to +40°C

• Höhe: bis zu 2000 m

• Relative Feuchte nicht über 95%

• Netzspannungsschwankungen nicht über 10%

• Überspannungsklasse 2 IEC 60364-4-443

• Verschmutzungsgrad 2

Hinweis: Die Gerätespezifikationen beziehen sich auf eine Umgebungstemperatur von 20°C und können sich außerhalb des Bereichs 10°C bis 30°C verschlechtern.

Das Gerät wurde auf HF-Störeinflüsse geprüft und entspricht den EMV-Bedingungen nach EN61326.

GARANTIE

Bibby Scientific Ltd gewährleistet, dass dieses Gerät für den auf der Garantiekarte angegebenen Zeitraum keine Herstellungs- und Materialmängel aufweist. Dieser Zeitraum tritt ab dem Verkaufsdatum in Kraft. Innerhalb dieses Zeitraums werden alle defekten Teile kostenlos ausgetauscht, soweit der Defekt nicht auf einen Unfall, Missbrauch oder Nachlässigkeit zurückzuführen ist. Die Anzeige des Thermocylers kan durch spitze/scharfe Gegenstände wie Stifte, Bleistifte und Fingernägel beschädigt werden. Diese Art von Beschädigung gilt als Missbrauch des Geräts und führt zur Ungültigkeit der Garantie für dieses Gerät.

Wartungsarbeiten, die unter diese Garantie fallen, müssen von der Verkaufsstelle für dieses Gerät gehandhabt werden.

Diese Anleitung wurde zur Information der Kunden von Bibby Scientific Ltd erstellt und stellt in keinster Weise eine Gewährleistung, Bedingung oder Darstellung bezüglich der Beschreibung, Marktgängigkeit oder Zweckdienlichkeit dieser Geräte oder Bauteile dar.

Unabhängig von Beschreibung und Spezifikation(en) des hier beschriebenen Geräts behält sich Bibby Scientific Ltd das Recht vor, Änderungen an diesem Gerät oder dessen Bauteilen vorzunehmen.

INFORMACIÓN DE SEGURIDAD E INSTALACIÓN

SEGURIDAD DEL OPERARIO

Lea atentamente este manual antes de utilizar el ciclador térmico. Si el equipo no se utiliza de la forma descrita en este manual, se reducirá la protección ofrecida por el equipo.

Todos los instrumentos Bibby Scientific Ltd están diseñados para cumplir los requisitos internacionales de seguridad, e incluyen un dispositivo de corte de sobretemperatura. Si se produce un problema de seguridad, apague la unidad en la toma de alimentación y retire el enchufe del suministro eléctrico.

ADVERTENCIA

LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que pueden ocasionar quemaduras graves a los operarios y prender el material combustible. Los usuarios deben conocer los posibles riesgos:

• TENGA CUIDADO Y LLEVE GUANTES DE PROTECCIÓN PARA PROTEGERSE LAS MANOS

• NO utilice sustancias combustibles cerca de objetos calientes

• NO utilice el instrumento cerca de líquidos o gases inflamables

• NO coloque un líquido directamente en el instrumento. Utilice siempre los consumibles plásticos adecuados

Después de calentar las muestras, recuerde que hay componentes del instrumento que pueden calentarse mucho, concretamente la tapa y el cuerpo y todos los accesorios asociados.

DESEMBALAJE

Durante el desembalaje, asegúrese de sacar los siguientes componentes del embalaje:

• Ciclador térmico

• Cable de alimentación

• Tarjeta de garantía

• Certificado de descontaminación

Se recomienda guardar el embalaje original en caso de que tenga que enviar el instrumento para un trabajo de mantenimiento o reparación. Bibby Scientific Ltd no se responsabiliza de los daños producidos si la unidad no está debidamente embalada y no se envía en su embalaje original.

Tenga en cuenta que los objetos punzantes, p.ej.: bolígrafos, lápices y uñas, pueden dañar fácilmente la pantalla del ciclador térmico. Este tipo de daño se considerará como un uso incorrecto, e invalidará la garantía de este componente.

INSTALACIÓN

El instrumento se debe transportar con las dos manos. No mueva ni lleve el instrumento cuando se utilice o esté conectado al suministro eléctrico principal.

1. Todos los instrumentos Bibby Scientific Ltd se suministran con un cable de alimentación, que puede ser integrado o 'enchufable'.

2. Antes de conectar el instrumento al suministro eléctrico, compruebe que el voltaje coincida con el indicado en la placa de régimen (situada en la parte trasera de la unidad). Asegúrese de que el interruptor de selección de voltaje (situado encima del interruptor de encendido/apagado) está ajustado al voltaje correcto del suministro eléctrico. El instrumento debe disponer de una toma de tierra para garantizar la seguridad eléctrica adecuada. (EI TC-3000X tiene una fuente conmutada que es conveniente para el uso con voltajes de entrada universales de CA entre 90 y 260V. No es necesario cambiar los fusibles al usar el TC-300X). Conecte el cable de alimentación a un enchufe adecuado según la siguiente tabla.

Conexión 220/240V, 50/60Hz Supply 110V/120V Supply, 50/60Hz

Con corriente marrón negro Neutro azul blanco Toma de tierra verde/amarillo verde

3. SÓLO PARA EL REINO UNIDO: El enchufe suministrado con el cable de alimentación incluye un fusible de 10 amperios para ofrecer protección al instrumento y al usuario.

4. Las unidades cuya placa de régimen indique 230V, 50/60Hz también pueden funcionar entre 210 y 260V; las unidades con 120V, 50/60Hz también funcionan entre 100 y 130V, y las unidades con 100V funcionan entre 90 y 110V. En todos los casos, el porcentaje de calentamiento disminuirá un 8% aproximadamente en los dos extremos del intervalo de voltaje.

5. Conecte el cable de alimentación en el enchufe situado en la parte trasera del instrumento.

6. Sitúe la unidad sobre una mesa plana o en una campana de laboratorio si es necesario, y asegúrese de que los orificios de ventilación situados en la parte inferior no tienen ninguna obstrucción. Coloque el instrumento a una distancia mínima de 200 mm alrededor de paredes u otros elementos, y entre cada unidad (en caso de que se utilicen varias) para que haya una circulación de aire adecuada para cada instrumento.

7. Encienda el instrumento:

I Interruptor de alimentación encendido

O Interruptor de alimentación apagado

Cable de repuesto

Si es necesario sustituir el cable de alimentación, se debe utilizar un cable de 1mm

de código armonizado

H05VV, conectado a un enchufe IEC320. EN CASO DE DUDA, PÓNGASE EN CONTACTO CON UN

ELECTRICISTA.

CONDICIONES DE TRABAJO

El ciclador térmico está diseñado para utilizarse en las condiciones siguientes:

• Uso en interior

• Intervalo de temperatura ambiente +5°C a +40°C

• Humedad relativa no superior al 95%

• Fluctuaciones del suministro eléctrico no superiores al 10%

• Categoría de sobrevoltaje II IEC 60364-4-443

• Nivel de contaminación 2

Nota: Las especificaciones de control corresponden a una temperatura ambiental de 20ºC. Las especificaciones pueden empeorar si se utiliza el instrumento fuera del intervalo de temperatura comprendido entre 10°C y 30°C.

Se han realizado pruebas para comprobar la interferencia de radiofrecuencia del instrumento, el cual cumple la normativa EN61326.

GARANTÍA

El instrumento está garantizado contra cualquier defecto en el material o la fabricación durante el período especificado en la tarjeta de garantía que se adjunta. Este período entra en vigor a partir de la fecha de compra. Durante este período, se reemplazarán sin cargo alguno todas las piezas defectuosas, a condición que el defecto sea resultado de un accidente, uso incorrecto o negligencia. Tenga en cuenta que los objetos punzantes, p.ej.: bolígrafos, lápices y uñas, pueden dañar fácilmente la pantalla del ciclador térmico. Este tipo de daño se considerará como un uso incorrecto, e invalidará la garantía de este componente.

El distribuidor del instrumento proporcionará información sobre las reparaciones realizadas bajo esta garantía.

Este manual se ha preparado con una finalidad informativa para los clientes de Bibby Scientific Ltd, y ninguna parte del manual se deberá considerar como una garantía, condición o reflejo con respecto a la descripción, comerciabilidad, idoneidad para un fin determinado o de otro tipo de la unidad o sus componentes.

Con independencia de la descripción y las especificaciones del instrumento que se indican en el manual del operario, Bibby Scientific Ltd se reserva el derecho de realizar cambios en el instrumento o en cualquiera de sus componentes cuando lo estime oportuno.

INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONE

SICUREZZA DELL'OPERATORE

Leggere attentamente il presente manuale prima di usare il ciclatore termico. Se non si usa l'apparecchiatura nel modo descritto nel presente manuale, la protezione fornita dall'unità potrebbe risultarne diminuita.

Tutti gli strumenti Bibby Scientific Ltd sono progettati per rispettare i requisiti di sicurezza internazionali e sono montati con un sezionatore di sovratemperatura. In caso di problemi di sicurezza, spegnere l'unità dalla presa di rete centrale e togliere la spina dall'alimentazione elettrica.

AVVERTENZA

Le ALTE TEMPERATURE SONO PERICOLOSE in quanto possono provocare serie ustioni agli operatori e dare fuoco al materiale combustibile. Gli utenti devono conoscere i seguenti pericoli potenziali:

• PRESTARE ATTENZIONE ED INDOSSARE GUANTI PROTETTIVI PER LE MANI

• NON usare sostanze combustibili vicino ad oggetti caldi

• NON mettere in funzione lo strumento nei pressi di liquidi o gas infiammabili

• NON collocare alcun tipo di liquido direttamente nello strumento, usare sempre le parti di consumo in plastica idonee

Dopo aver riscaldato i campioni, ricordare che le parti dello strumento, cioè il coperchio ed il blocco ed eventuali accessori associati potrebbero essere molto caldi.

DISIMBALLAGGIO

Durante il disimballaggio, assicurarsi di aver tolto quanto segue dall'imballo:

• Ciclatore termico

• Cavo di alimentazione di rete

• Scheda di garanzia

• Certificato di decontaminazione

Si consiglia all'utente di conservare l'imballaggio originale nel caso in cui occorresse restituire lo strumento per assistenza o riparazioni. Se l'unità non è correttamente imballata e trasportata nel suo imballo originale, Bibby Scientific Ltd non accetta alcuna responsabilità per eventuali danni che dovessero verificarsi.

Notare come lo schermo del ciclatore termico si danneggia facilmente con oggetti appuntiti come penne, matite ed unghia. Questo tipo di danni sarà considerato come uso improprio e renderà nulla la garanzia per questo componente.

INSTALLAZIONE

Occorre trasportare lo strumento usando entrambe le mani. Non spostare né trasportare lo strumento quando è in funzione o collegato all'alimentazione elettrica di rete.

1. Tutti gli strumenti Bibby Scientific Ltd sono forniti con un cavo di alimentazione; può essere integrale o plugin.

2. Prima di collegare lo strumento alla rete elettrica controllare il voltaggio riportato sulla etichetta sul retro dell'unità. Assicurarsi che l'interruttore di selezione del voltaggio (posot al di sopra dell'interruttore on/off) sia impostato sul voltaggio idoneo per la rete di alimentazione locale. prendere nota che l'unità deve essere messa a terra per garantire la sicurezza elettrica. ( il modello TC-3000X dispone di alimentazione idonea per voltaggi in corrente alternata tra 90 e 260V. Non è necessario cambiare il fusibile utilizzando il TC-3000X) Collegare il cavo di alimentazione ad una spine idonea in accordo alla tabella sottoriportata.

Connessione 220/240V, Alimentazione 50/60Hz Alimentazione 110V/120V, 50/60Hz

Sotto tensione marrone nero Neutro blu bianco Te rra verde/giallo verde

3. SOLO REGNO UNITO: la spina con fusibile fornita con il cavo di rete è dotata di un fusibile da 10 Amp per proteggere lo strumento e l'utente.

4. Le unità la cui targhetta dei valori nominali indica 230V, 50/60Hz funzionano anche tra 210 e 260V; quelle con 120V, 50/60Hz funzionano anche tra 100 e 130V e quelle contrassegnate da 100V funzionano tra 90 e 110V. In tutti i casi, il tasso di riscaldamento diminuirà di circa l'8% agli estremi dell'intervallo di tensione.

5. Inserire il cavo di rete nella presa che si trova sul retro dello strumento.

6. Collocare l'unità su un banco piatto idoneo o in una cappa aspirante se necesario, assicurandosi che gli sfiati delle prese d'aria nella parte inferiore non siano ostruiti. Posizionare lo strumento con una distanza minima attorno di 200 mm. dalle pareti o da altri oggetti e tra ciascuna unità, nel caso in cui si usino unità multiple, in modo da consentire un flusso di'aria efficace per ciascuno strumento.

7. Accendere lo strumento:

I Interruttore di rete Acceso

O Interruttore di rete Spento

Cavo di ricambio

Qualora occorra sostituire il cavo di rete, si dovrà utilizzare un cavo di 1mm

codice armonizzato H05VV-F

collegato ad una spina IEC 320. IN CASO DI DUBBIO, RIVOLGERSI A UN ELETTRICISTA

QUALIFICATO.

CONDIZIONI DI ESERCIZIO

Il ciclatore termico è stato progettato per funzionare nelle seguenti condizioni:

• uso interno

• range di temperatura ambiente da +5°C a +40°C

• altitudine massima 2000 m.

• umidità relativa non superiore all'95%

• oscillazione dell'alimentazione di rete non superiore al 10%

• categoria di sovratensione II IEC 60364-4-443

• grado di inquinamento 2

Nota: le specifiche di controllo sono indicate ad una temperatura ambiente di 20°C. Le specifiche potrebbero peggiorare fuori da una temperatura ambiente compresa tra 10°C e 30°C. Lo strumento è stato collaudato per interferenze da radiofrequenze ed è certificato secondo la norma EN61326.

GARANZIA

Lo strumento è garantito da qualsiasi difetto nei materiali o nella lavorazione per il periodo specificato nella scheda di garanzia allegata. Questo periodo è valido dalla data di acquisto; entro tale periodo, tutte le parti difettose saranno sostituite gratuitamente, a condizione che il difetto non sia la conseguenza di un incidente, un uso improprio o negligenza. Lo schermo del ciclatore termico può essere danneggiato da oggetti appuntiti come penne, matite ed unghia. Tale tipo di danno sarà considerato uso improprio e renderà nulla la garanzia per questo componente.

L'assistenza secondo quanto stabilito dalla presente garanzia deve essere ottenuta dal fornitore dello strumento.

Il presente manuale è stato preparato ad uso dei clienti di Bibby Scientific Ltd e niente di quanto in esso contenuto costituisce garanzia, condizione o rappresentanza riguardo la descrizione, la commerciabilità, l'idoneità allo scopo o altrimenti dell'unità o dei componenti.

Nonostante la descrizione e le specifiche dello strumento contenuti nel manuale dell'operatore, Bibby Scientific Ltd si riserva il diritto di apportare le modifiche ritenute opportune agli strumenti o a qualsiasi loro componente.

BEFORE SWITCHING ON

Ensure that the voltage selector switch is set to the appropriate voltage for your supply;

On/Off switch

Ensure that the appropriate fuses are fitted for your supply:

• For 230V set the voltage selector switch set to 230V and fit T2.5A fuses.

• For 115V set the switch to 115V and fit T4A fuses.

• For 100V the switch will be set to 115V T4A fuses will be fitted.

USB

On/Off switch

Power in - no requirement to change fuses as a switch-mode power supply is fitted.

TC-3000

TC-3000X

CONTACT INFORMATION

For technical, sales or servicing information, contact your local Bibby Scientific Ltd dealer or:

UK and Rest of World

Bibby Scientific Ltd Beacon Road, Stone Staffordshire ST15 0SA, UK Tel: +44 (0)1785 812121 Fax: +44 (0)1785 813748 e-mail: techne@bibby-scientific.com www.bibby-scientific.com

France

Bibby Scientific France SAS ZI du Rocher Vert - BP 79 77793 Nemours Cedex France Tel: +33 1 64 45 13 13 Fax: +33 1 64 45 13 00 e-mail: bsf@bibby-scientific.fr www.bibby-scientific.com

Middle East

Techne Middle East Ltd. PO Box 27887, Engomi 2433 Nicosia Cyprus Tel: +357 22 660427 Fax: +357 22 660356 e-mail: sales@techneme.com

North and South America

Techne Inc. 3 Terri Lane, Suite 10 Burlington, N.J. 08016, USA Tel: 800-225-9243 Fax: 609-589-2571 e-mail: labproducts@techneusa.com www.techneusa.com

Italy

Bibby Scientific Italia Srl Via Alcide de Gasperi 56 20077 Riozzo di Cerro al Lambro Milano, Italia Tel: +39 (0)2 98230679 Fax: +39 (0)2 98230211 e-mail: marketing@bibby-scientific.it www.bibby-scientific.it

Far East

Bibby Scientific (Asia) Ltd Room 607 Yen Sheng Centre 64 Hoi Yuen Road Kwun Tong Kowloon Hong Kong Tel: +852 3583 1581 Fax: +852 3583 1580 e-mail bibby@bibby-scientificasia.com www.bibby-scientificasia.com

TC-3000, TC-3000X SPECIFICATIONS

TC-3000 TC-3000X

Temperature

Temperature range 4°C to 99°C 4°C to 99°C Temperature set point precision 0.1°C 0.1°C Block uniformity (over full range) ± 0.1°C ± 0.2°C Temperature accuracy ± 0.2°C ± 0.2°C End of program cooling below ambient Yes Yes

Heating/Cooling Rate

Heating rate, between 55°C and 90°C on a 50°C to 95°C step:

0.2 ml micro tubes 3.6°C/sec 3.3°C/sec

0.5 ml micro tubes 3.6°C/sec 3.3°C/sec Cooling rate, between 90°C and 55°C on a 95°C to 50°C step:

0.2 ml micro tubes 2.0°C/sec 2.0°C/sec

0.5 ml micro tubes 1.8°C/sec 2.0°C/sec

Heated Lid

Adjustable heated lid No Yes Heated lid enable/disable Yes Yes Selectable heated lid temperature 100°C to 115°C 100°C to 115°C Over-temperature cut-out Fixed at 145°C Fixed at 145°C Temperature sensor Thermistor Thermistor Heater type Etched foil Etched foil Heater power 33W 33W Warm up time 4 min approximately 4 min approximately

(ambient to 105°C) (ambient to 105°C) The heated lid will only come on if the set temperature is above 35°C

Programming

Program naming using alpha keys Yes Yes Program password protection Yes Yes Number of programs (3 step) 80 80 Maximum number of steps per stage 35 35 Maximum number of stages per program 25 25 Minimum hold time 1 sec 1 sec Maximum hold time 18 hrs 18 hrs Incremental/decremental temperature Yes Yes Incremental/decremental step hold time Yes Yes Programmable ramp rate (heat or cool) Variable in 0.1°C/sec steps Variable in 0.1°C/sec steps Programmable ramp rate resolution 0.1°C 0.1°C

TC-3000 TC-3000X

Running Programs

Pause button Yes Yes Stop button Yes Yes End of program alarm Yes Yes Auto restart on power fail Yes Yes

Interconnectivity

Connection to PC control program Yes Yes Software updates from Techne web-site Yes Yes

Dimensions

Height 185mm 185mm Width 185mm 185mm Length 335mm 335mm

Communication Ports

PC connection to run "Gensoft" RS232 serial USB the PC control software.

Power

Power consumption 230W 230W

The TC-3000 units are voltage selectable by a slide switch. When the unit is set to 115V it will work at any voltage between 110V and 130V. When the unit is set to 230V it will work at any voltage between 210V and 260V. Units marked 100V will work at any voltage between 90 and 110V. The performance will vary and will not necessarily meet the above typical specification at the extremes of voltage. The TC-3000X unit is supplied with a switched mode power supplier so the unit automatically recognises the input voltage.

HEATED LID: TC-3000

To r elease the heated lid, lift the lid catch and the lid will open. To close the lid, push down gently until the catch clicks in place. Do not close the

lid without tubes being fitted in the block.

HEATED LID: TC-3000X

The heated lid has a rotating knob to adjust the lid pressure on the samples, allowing for a variety of consumables to be used. To adjust the pressure:

• Adjust the orange rotating knob anticlockwise to give the least pressure.

• Place the samples in the block and place the lid in the down position.

• Gently rotate the knob clockwise until you can feel pressure beginning to be applied.

• Rotate the knob a further quarter of a turn and this will give the correct pressure.

USER OPERATION AND CONSUMABLES

Do not over-tighten the knob as this will cause deformation of the silicone rubber mat (where used) and reduce the effectiveness of the lid. Once the pressure has been set it does not need to be adjusted unless a different block or consumable is used.

The silicone rubber mat supplied with the unit (Part number 6106196) is for use with 48 well microplates when sealing using a plastic adhesive film. The mat should be placed on top of the sealing film to ensure good thermal contact with the heated lid.

TUBES OR REACTION VESSELS

Bibby Scientific Ltd does not recommend any specific tube or reaction vessel other than those described in this manual. We recommend using reaction volumes between 20 and 200µl. The tubes must withstand a pressure of 1 atmosphere at 100°C. Any vessel must be able to withstand the temperatures you are using without any danger of them deforming to the point where they fracture.

To test your tubes, put 25µl of water into each of 5 tubes and subject them to a typical thermal cycling protocol. At the end of the program, measure the volume remaining using a micropipette. A loss of more than 1 or 2µl indicates a vapour leak.

The amount of volume loss you observe and the change in reactant concentrations you can tolerate determine the minimum volume that can be used. Typical volume losses of 1µl in 30 cycles allow the use of samples of 20µl or less.

During the final cool-down, a ring of condensation may form above the liquid level but below the top of the sample block. This is normal and does not effect the reaction.

0.5ML TUBES

The heated lid mechanism of the TC-3000 with the 0.5ml block is optimised for flat top tubes; it is not suitable for use with domed cap tubes. In order to fit the maximum number of tubes in the block, they need to be placed so that all the hinges are in the same relative orientation. An example is illustrated below.

PLATES AND SEALS

The 48 x 0.2ml block of the TC-3000X will accept 48-well plates as well as individual and strip 0.2ml tubes. To avoid the possibility of sample evaporation Bibby Scientific Ltd recommends that plates are sealed with appropriate caps or heat seals. We do not recommend the use of adhesive seals, but if their use is unavoidable then the supplied silicone rubber mat should be placed on top of the sealing film to ensure good thermal contact with the heated lid.

Place the tubes in the block in the same orientation in order to achieve the maximum capacity.

Always make sure the pressure on the heated lid is balanced by spreading the tubes across the block or inserting some dummy tubes if necessary.

When you switch on the unit a screen similar to the one shown here will appear briefly. This indicates the software version and block type installed in the unit.

When the initial screen has disappeared the screen shown here will automatically appear. There are three options:

1. 'Programs' for creating and running programs;

2. 'Information' for obtaining information about the unit e.g. serial number;

3. 'Configuration' for setting up program defaults and settings.

Each of these is explained in detail in the following sections.

TC-3000

Version x.xx Block 25x0.2ml

Programs [0]

Information Configuration [ , to select ]

SWITCHING ON

FRONT PANEL CONTROLS

THE LCD DISPLAY

INDICATORS

Power: This indicator is lit when there is power to the TC-3000 or TC-3000X.

Status: This indicator is lit when the unit is running a program. It is off when the unit is

stopped. It will flash and beep slowly when the unit is paused. It will flash after a five second quick sequence of beeps when the unit reaches its final hold temperature.

pqrs8tuv9wxyz

TC-3000

ghi5jkl6mno

1 2

abc3def

Programs [0]

Information Configuration [ , to select ]

Operation or Program lines

Decimal Point & Insert

Delete

Pause

Status

Enter

Prompt line

End

Down

Power

Alpha/Numeric keys

The TC-3000 & TC-3000X have a four line LCD display. The top three lines are information or program lines, while the bottom line is a prompt line. The active line flashes on screen and is shown in bold in this manual.

The bottom line may change or flash between two or three different prompts depending upon which program line is active. An example of a prompt line which flashes between three different prompts is shown here.

Programs [0]

Information Configuration [ , to select ]

-------- Stage 1 --------Number cycles 10

Step 94.0° C 00m30 max

[ '.' to insert step ]

[ , to edit ] [ to delete step ]

The "End" or "Finish" key is used to finish editing programs or to exit a program. It is also used to exit a sequence of key operations.

The "Up arrow" key is used for scrolling up through the lines on the screen. It is also used to increase certain fields when editing.

The "Down arrow" key is used for scrolling down through the lines on the screen. It is also used to decrease certain fields when editing.

The "Decimal Point" key is used when editing numeric fields. It is also used as an Insert key during program editing to insert additional steps and other field sets.

The "Delete" key is used during program editing to delete steps and stages.

The "Pause" key is used during program editing to insert a pause after a stage or to insert increment and decrement times and temperatures. It is also used to pause a program when it is running.

The "Enter" key is used to enter an operation or the edit mode. It is also used to 'Accept' a field or program change.

The "Alpha/Numeric" keys are used to enter numbers and letters during programming. If it is a numeric field then numbers will be inserted; if it is an alpha field then letters will be inserted. Each key will cycle through the letters shown on the key (0 will insert a space). To insert a number, press and hold the appropriate key.

The End, Finish or Exit key

The Up arrow key

The Down arrow key

The Decimal Point and Insert key

The Delete key

The Pause key

The Enter or Accept key

The Alpha/Numeric keys

pqrs8tuv9wxyz

ghi5jkl6mno

1 2

abc3def

THE KEYS

PROGRAMMING THE TC-3000 & TC-3000X

The table below illustrates the symbols which appear on the LCD display and the relevant keypad button to which they refer.

In the prompt line is equivalent to the "End" key.

On a program line means the program is locked; it can be copied and then edited but not edited directly.

Up arrow

Down arrow

Enter

Pause

The following terms are used when programming the thermal cyclers:

Stage A 'Stage' is the name given to the main repeated part of a program containing the 'Steps' to be

repeated. A program can contain a maximum of 25 stages although typically only one or two are used.

Cycle A 'Cycle' is the number of repetitions of a 'Stage'

Step A 'Step' is a user defined program line containing a temperature, a hold time and ramp rate.

Typically three steps are created within one 'Stage'.

To enter the programming section of the software, highlight 'Programs' using the "Up Arrow" or "Down Arrow" keys

Press "Enter"

This screen lists all the programs saved on the unit. Use the "Up Arrow" and "Down Arrow" keys to scroll through the list.

The number next to the program name is the unique "Speed dial" number allocated to the program for rapid retrieval (see below).

Programs [0]

Information Configuration [ , to select ]

* * New Program * * [ ]

2 STEP TEMPLATE 501 3 STEP TEMPLATE 502 [ , to select ]

PROGRAMMING OPTIONS

There are two options available for creating programs on the TC-3000 & TC-3000X:

1. Copy and edit one of the pre-written templates.

2. Create a new program from the defaults.

OPTION 1: CREATING PROGRAMS USING TEMPLATES

There are two pre-written basic PCR templates: a two-step and a three-step template. These can be copied and modified as required.

Other templates available include Ice bucket (incubation at 4ºC), Ligation (incubation at 15ºC) and an RTPCR template.

Use the "Down Arrow" key to select an existing 'Template' program from the menu, e.g. '2 Step Template' or '3 Step Template'.

Press the "Enter" key to accept it.

The Template is already defined and can be run as it is or easily copied, modified and saved under a new name.

Use the "Down Arrow" key to select 'Copy Program 'Press the "Enter" key to accept it.

2 STEP TEMPLATE [501]

Run program

View program [ , to select ]

2 STEP TEMPLATE

Heated Lid 105°C Preheat Lid on Pause off

------------------------In denat 94°C 5m00 Hot Start off

------- Stage 1--------Number cycles 30 Step 94.0°C 0m30 max Step 68.0°C 1m00 max

------------------------Final Extn 72.0°C 5m00 Final hold 10.0°C

3 STEP TEMPLATE

Heated Lid 105°C Preheat Lid on Pause off

------------------------In denat 94.°C 5m00 Hot Start off

------- Stage 1--------Number cycles 30 Step 94.0°C 0m30 max Step 55.0°C 0m30 max Step 72.0°C 0m30 max

------------------------Final Extn 72.0°C 5m00 Final hold 10.0°C

Run program View program

Copy program

[ , to select ]

* * * New Program * * *

2 STEP TEMPLATE 501

3 STEP TEMPLATE 502 [ , to select ]

To save the program

[ to save ]

[ YES or NO ]

To give a program a name

A new name can now be given to the copied program (see below).

To type in a name for the program, press "Enter" and a cursor will appear in the position shown.

Using the keypad, type in the name you wish to give the program.

The letters will scroll round with successive presses; for example the "2" key will give 'A' with one press; 'B' with two presses; 'C' with three presses and '2' with four presses. Five presses will again give 'A'.

When the name is complete, press "Enter"

There are now two options:

1. Save the program and run it as it is or

2. Edit the program then save and run it.

A program can only be run once it has been saved. Press the "End" key.

The prompt line will flash between 'SAVE?' and 'YES or NO'.

Press "Enter" for 'Yes' to save the changes you have made.

If you press "End" for 'No' then all the changes you have made will be lost.

The program is now ready to run and will be allocated with a unique 'Speed dial' number which can be used to rapidly locate the program from the main start-up screen.

“Up Arrow” or “Down Arrow” keys to scroll through the remaining list of options.

UNNAMED #

Heated Lid 105°C Preheat Lid on [ , to edit ]

UNNAMED #

Heated Lid 105°C Preheat Lid on [ , to edit ]

DONNA

Heated Lid 105°C Preheat Lid on [ , to edit ]

DONNA

Heated Lid 105°C Preheat Lid on [ SAVE? ]

DONNA [7]

Run Program

View Program [ , to select ]

1 2

abc3def

ghi5jkl6mno

pqrs8tuv9wxyz

Edit a selected field or step

DONNA

Heated Lid 105°C Preheat Lid on [ , to edit ]

To edit a field or step in an existing program

When the unit is switched on the display will show this screen.

If known, type in the 'Speed dial' number for the required program and press "Enter" This will take you directly to the required program.

If you do not know the 'Speed dial' number, press "Enter" to access the list of stored programs.

Either:

1. Scroll to the required program using the

"Up Arrow" or "Down Arrow" keys

2. Type the first letter of the program name in

the 'New program' field; this will then list all the program names beginning with that letter. Use the "Up Arrow" or "Down Arrow" keys to select the required program.

Press "Enter" to accept

The full set of options for a program is shown here; these are shown on the LCD screen three lines at a time.

Use the "Down Arrow" and/or the "Up Arrow" keys to select the option you require.

Press the "Enter" key to select the option.

Select 'Edit program' and a screen similar to this will appear.

Use the "Down arrow" and/or the "Up arrow" keys to select the field or step you want to edit.

You can now edit the active field or step by first pressing the "Enter" key

Programs [0]

Information Configuration [ , to select ]

DAVID 8 [D]

DONNA 7

[ , to select ]

DONNA [7] Run program View program Edit program Copy program Delete program Set password [ , to select ]

[ to save ]

[ to disable ]

[ turn on/off ]

To edit a temperature, type in the required temperature. In the example here, the heated lid temperature is being edited.

Press "Enter" to accept.

Use the "Down Arrow" and/or the "Up Arrow" keys to select the next field or step you wish to edit.

To edit a field with on/off settings, first select the field and press "Enter"

Use the "Up Arrow" and "Down Arrow" keys to toggle between the settings.

Press "Enter" to accept.

Sample cooling is a new function on the TC-3000X that enables to be actively cooled to 4°C whilst the heated lid is reaching the correct temperature.

To edit a step with both a temperature and a time, first press "Enter" and type in the new temperature. Press "Enter" again. Now type in the new time. The default is minutes.

1. For whole minutes, type the number

required then "Enter" .

2. To toggle between minutes and hours, use

the "Decimal Point" key.

3. For values less than 1 minute, first press the

"Decimal Point" key followed by the number of seconds required.

4. For minutes and seconds, type the number

of minutes followed by the "Decimal Point" key then the number of seconds.

Once the values have been added, press "Enter" to accept.

Once you have finished editing and you are happy with the changes, press the "End" key

followed by the "Enter" key to save.

If you press the "End" key a second time for 'No' then all the changes you have made will be lost.

DONNA

Heated Lid 105°C

Preheat Lid on [ edit temperature ]

DONNA

Heated Lid 105°C

Preheat Lid on

[ to change ]

[ to disable ]

[ .for hrs/mins ]

Pause off

-------------------------

In denat 4.0°C 00 m30

[ edit temperature]

Pause off

-------------------------

In denat 94.0°C 0m30

[ edit time ]

------------------------Fin extn 72.0°C 5m00 Fin hold 10.0°C [ , to edit ]

[ to save ]

DONNA

Preheat Lid on

Sample cooling off

[ Yes or No ]

To delete a selected program

OPTION 2: CREATING A NEW PROGRAM

A second option is to create a completely new program without using templates.

If you select 'Copy program' a screen similar to this will appear and the software will automatically give the program the next number until it is named.

Press "Enter" and type in a new name. You will then be able to edit the program as previously described.

If you select 'Delete program' a screen similar to this will appear.

Press "Enter" to delete the program or "End" to return to the program screen.

If you want to password protect the program so that other users cannot edit it, select 'Set password'. A screen similar to this will appear. Type in the password you wish to give it and press "Enter" . The program list will then display the key symbol next to the name to show that it is locked. Other users will still be able to copy/view your program but not edit it.

A number of typical values are pre-entered including:

Heated lid temperature Preheat lid Sample cooling (TC-3000 shown in diagram) Initial denaturation Hot start Final extension Final hold

These values can be edited and the additional stages and steps required for cycling can be added by following the instructions detailed on the following pages.

UNNAMED #

Heated Lid 105°C Preheat Lid on [ , to edit ]

DONNA

delete program

SURE?

DONNA

New passwd

[ to accept ]

UNNAMED #

Heated Lid 105°C Preheat Lid on Pause off

------------------------In denat 94.°C 5m00 Hot Start off

------------------------Final Extn 72.0°C 5m00 Final hold 10.0°C

To copy a selected program

To set a password for a selected program

[ to save ]

[ to exit ]

EXAMPLE PROGRAMS

A couple of example thermal cycling programs are shown below.

Typical 3-Step PCR 3-Step PCR with multiple 'Stages'

PROGRAMMING

To add a stage and then edit it

Heated Lid 105°C Preheat Lid on Pause off

------------------------In denat 94°C 5min Hot Start off

-------- Stage 1 -------Number cycles 30 Step 94.0°C 0m30 max Step 55.0°C 0m30 max Step 72.0°C 0m30 max

------------------------Final Extn 72.0°C 5m Final hold 4°C 10m

Heated Lid 105°C Preheat Lid on Pause off

------------------------In denat 94°C 5min Hot Start off

-------- Stage 1 -------Number cycles 10 Step 94.0°C 0m30 max Step 60.0°C 0m30 max Step 72.0°C 0m30 max

------------------------Number cycles 20 Step 94.0°C 0m30 max Step 55.0°C 0m30 max Step 72.0°C 0m30 max

------------------------Final Extn 72.0°C 5m Final hold 4°C 10m

Create a new program by selecting ** New Program ** from the menu and press "Enter"

Give the program a name and edit any of the default fields as described above.

Use the "Down Arrow" key to scroll to the dotted line below 'Hot Start'; it will start to flash.

Press "Enter" to insert a stage.

* * New Program * * [ ]

2 STEP TEMPLATE 501 3 STEP TEMPLATE 502 [ , to select ]

UNNAMED #

Heated Lid 105°C Preheat Lid on [ , to edit ]

In denat 94.0°C 5m00 Hot Start off

------------------------[ to insert stage ]

[ to save ]

[ , to edit ] [ to delete step ]

[ , to edit ]

To insert additional steps

A new stage will be inserted which consists of a 'Number cycles' field and one step.

Edit the number of cycles by typing the number required then press "Enter"

Use the "Down Arrow" key to move to the step.

Press "Enter" to edit the step as described above.

Press "Enter" again to accept.

To insert a new step before the first step, use the "Up Arrow" or "Down Arrow" keys to select 'Number cycles'.

Press the "Decimal Point" key to insert a new step.

To insert a new step after an existing step, use the "Up Arrow" or "Down Arrow" keys to select the step.

Press the "Decimal Point" key to insert a new step.

Use the "Up Arrow" or "Down Arrow" keys to select the step you wish to delete.

Press "Delete" to delete the step.

The prompt line will flash between 'DEL STEP?' and 'YES or NO'.

Press "Enter" to delete the step.

Press "End" to return to editing the step.

-------- Stage 1 -------Number cycles Step 94.0° C 00m30 max

[ edit no.cycles ]

-------- Stage 1 -------Number cycles 10

Step 94.0° C 00m30 max

[ '.' to insert step ]

-------- Stage 1 -------Number cycles 10

Step 94.0° C 00m30 max

[ '.' to insert step ]

-------- Stage 1 -------Number cycles 10

Step 94.0° C 00m30 max

[ DEL STEP ? ]

To delete a step

-------- Stage 1 --------

Number cycles 10

Step 94.0° C 00m30 max [ '.' to insert step ]

[ , to edit ] [ to delete step ]

[ Yes or No ]

[ to delete stage ]

------------------------Final Extn 72.0°C 5m00 Final hold 10.0°C [ to insert stage ]

Use the "Up Arrow" or "Down Arrow" keys to highlight the stage number.

Press "Enter"

A new stage will be added before the existing stage. The other stage numbers will be adjusted as appropriate. The new stage can now be edited as described above.

Use the "Up Arrow" or "Down Arrow" keys to highlight the dotted line below the last stage.

Press "Enter"

A new stage will be added with the next consecutive number. The new stage can now be edited as described above.

Use the "Up Arrow" or "Down Arrow" keys to highlight the stage you wish to delete.

Press "Delete"

The prompt line will flash between 'DEL STAGE?' and 'YES' or 'NO'.

Press "Enter" to delete the stage.

To add a new stage before an existing one

To add a new stage at the end

To delete a stage

-------- Stage 1 -------Number cycles 10 Step 94.0° C 00m30 max [ to insert stage ]

[ to delete stage ]

-------- Stage 1 -------Number cycles 10 Step 94.0° C 00m30 max [ to insert stage ]

-------- Stage 1 -------Number cycles 10 Step 94.0° C 00m30 max [ DEL STAGE? ]

[ Yes or No ]

[ ‘.’ to insert step ] [ , to edit ]

TO PROGRAM INCREMENT OR DECREMENT TEMPERATURE OR TIME

Under normal circumstances, the Hold Temperature of all steps is constant. However, it is possible to automatically increment or decrement the temperature of a specified step of a programme.

Temperature profile of incremented Hold Temperature at step 3 of each cycle

To insert a pause after an existing stage, follow the steps for inserting a stage as above.

Highlight the 'Number cycles' line then press "Pause"

The prompt line will flash between 'CHANGE TO PAUSE?' and 'YES or NO'.

Press "Enter" to insert a pause.

The stage number will then be highlighted with 'Pause' as the only line.

To insert a ‘pause’ into a program

Step 94.0° C 00m30 max

-------- Stage 2 --------

Number cycles

Step 94.0° C 00m30 max

[ CHANGE TO PAUSE ] [ Yes or No ]

Step 94.0° C 00m30 max

-------- Stage 2 --------

Number cycles 0

Step 94.0° C 00m30 max

-------- Stage 2 -------Pause

------------------------[ to insert step ]

Cycle 1

22222

Cycle 2 Cycle 3 Cycle 4 Cycle 5

+4∂

+3∂

+2∂

O+∂O

Temperature

[ to delete step ]

The Hold Temperature of the incremented/decremented step is the defined Hold Temperature plus the summation of the increments/decrements. The first cycle is never incremented/decremented, only subsequent cycles. If you select a decrement, the Hold Temperature is prevented from falling below 4°C. If you select an increment, the Hold Temperature is prevented from rising above 99°C.

Similarly, under normal circumstances, the Hold Time of all steps is constant. However, it is possible to automatically increment or decrement the duration of a specified step of a program.

Temperature profile of incremented Hold Time at step 3 of each cycle

The Hold Time of the incremented/decremented step is the defined Hold Time plus the summation of the increments/decrements. The first cycle is never incremented/decremented, only subsequent cycles. If you select a decrement, the Hold Time is prevented from falling below one second.

Use the "Up Arrow" or "Down" Arrow" keys to move to the step where you want an increment or decrement temperature or time

Press "Enter" to go into edit mode.

Press "Pause"

The prompt line will change to flash between 'CHANGE STEP?' and 'YES' or 'NO'.

Press "Enter"

The cursor will again move into the edit mode.

Now press the "Up Arrow" key

Number cycles 30 Step 94.0° C 00m30 max

Step 58.0° C 00m30 max

[ , to edit ]

Number cycles 30 Step 94.0° C 00m30 max

Step 58.0° C 00m30 max

[ CHANGE STEP ? ]

Cycle 1

Cycle 2 Cycle 3 Cycle 4 Cycle 5

+3∂

Temperature

+4∂

+2∂

tt+∂t

[ Yes or No ]

[ to delete step ] [ ‘.’ to insert step ]

Use the "Alpha/numeric" keys to change the values of the 'fst' line to the first temperatures and/or times you require.

Press "Enter" after each field you change.

You will exit 'Edit Mode' when you reach the end of the line.

Use the "Down Arrow" key to move onto the 'lst' line.

Press "Enter" to edit the step.

Use the "Alpha/numeric" keys to change the values of the 'lst' line to the final temperatures and/or times you require.

Press "Enter" for each field you change.

You will exit 'Edit Mode' when you reach the end of the line.

Number cycles 30 Step 94.0° C 00m30 max

fst 65°C 00m30 max

[ edit temperature ]

Step 94.0° C 00m30 max fst 65.0° C 00m30 max

lst 55.0° C 00m30

[ , to edit ]

Step 94.0° C 00m30 max fst 65.0° C 00m30 max

lst 55.0° C 1m30

[ edit time ]

Number cycles 30 Step 94.0° C 00m30 max

fst 55.0° C 00m30 max

[ , to edit ]

The step will change to 'fst', meaning "first", and the software will insert another line after it entitled 'lst', meaning "last".

Press "Enter" twice to go into edit mode.

When you run the program it will automatically increment or decrement in equal steps from the first time and/or temperature to the last over the number of cycles in the stage. For example: if the first temperature is 65°C and the last temperature is 55°C, over 30 cycles the temperature will decrease

0.33°C each cycle. Similarly, in the example shown, the time would increase from 30 seconds to 1 minute 30 seconds in 2 second increments.

[ to save ]

[ to delete step ] [ ‘.’ to insert step ]

[ .For hrs/mins ]

At any time during programming the TC-3000 or TC-3000X, when you are satisfied with the program, you can finish and accept what you have done by first pressing the "End" key.

Then follow the instructions on screen. If you are not sure, wait for the instructions in the prompt line to scroll back to the first line that you see.

To save the program

Stopping what you are doing

A program can only be run once it has been saved. Press the "End" key.

The prompt line will flash between 'SAVE?' and 'YES or NO'.

Press "Enter" for 'Yes' to save the changes you have made.

If you press "End" for 'No' then all the changes you have made will be lost.

The program is now ready to run and will be allocated with a unique "Speed dial" number which can be used to rapidly locate the program from the main start-up screen.

Use the "Up Arrow" and "Down Arrow"

keys to scroll through the list of options.

-------- Stage 1 -------Number cycles 10 Step 94.0° C 00m30 max [ SAVE? ]

GEORGE (10)

Run Program

View Program [ , to select ]

[ Yes or No ]

TO RUN A PROGRAM

When the unit is switched on the display will show this screen.

If known, type in the 'Speed dial' number for the required program and press "Enter" This will take you directly to the required program.

If you do not know the 'Speed dial' number, press "Enter" to access the list of stored programs.

Next either:

1. Scroll down to the required program using

the "Up Arrow" and "Down Arrow" keys.

2. Or type the first letter of the program name

in the "New program" field; this will then list all the program names beginning with that letter. Use the "Up Arrow" and "Down Arrow" keys to select the required program.

Press "Enter" to accept For example 'Donna'.

To run the program, select 'Run program' and press "Enter"

A screen similar to this one will appear.

Press "Enter" to start the run.

Programs [0]

Information Configuration [ , to select ]

Programs [7]

Information Configuration [ , to select ]

* * New Program * * [ ]

2 STEP TEMPLATE 501 3 STEP TEMPLATE 502 [ , to select ]

DAVID 8 [D]

DONNA 7

[ , to select ]

DONNA [7]

Run Program

View Program [ , to select ]

DONNA

[ RUN PROGRAM? ]

[ Yes or No ]

[ to CONTINUE ] [ for info ]

During the run the LCD screen will report:

1. The cycle number

2. The time remaining (counting down in

seconds)

3. The sample temperature (whether holding

or ramping)

4. The current step of the run

5. A count down of the hold time.

An example is shown here.

To pause a program while it is running, press "Pause" The run will continue but the prompt line will alternate 'PAUSE?' and 'YES or NO'

To confirm that you want the program to pause press "Enter"

If you have pressed the "Pause" key by mistake and you want the program to continue, press "End"

The unit will emit a beeping sound while paused.

To r esume the run, press "Pause" followed by "Enter" to continue.

To stop a program while it is running, press "End"

The run will continue but the prompt line will alternate 'STOP PROGRAM?' and 'YES or NO'

To confirm that you want to stop the program press "Enter"

If you have pressed the "End" key by mistake and you want the program to continue, press "End" again.

When a program is stopped the unit will emit a beeping sound and the original program screen will appear.

Manual pause

Manual stop

Cycle 4/30 56m08 Sample 58.0°C Hold @ 58.0°C 00m17 [ to STOP ]

Cycle 24/30 19m26 Sample 72.0°C Hold @ 72.0°C 00m10 [ PAUSE? ]

Cycle 24/30 19m26 Sample 72.0°C Paused [ to STOP ]

Cycle 24/30 10m39 Sample 94.0°C Hold @ 94.0°C 00m10 [ STOP PROGRAM? ]

[ Yes or No ]

[ to PAUSE ] [ for info ]

CONFIGURATION

Password pad [ to accept ]

Program Information

INFORMATION

CONFIGURATION

Warning! The Configuration software should only be accessed by designated personnel, as some

parameters can be changed or may be lost when using it.

Pressing "Enter" while a program is running will give information about the current run including which program is actually running and the unit status e.g. holding or ramping.

An example is shown here.

The Information field on the front screen contains all the unit-specific information such as serial number and software version.

Press "Enter" to access the unit information.

The complete list is displayed here.

Note that the "Comms address" must be unique for each unit if more than one cycler is linked to a PC using the Gensoft control software.

The Configuration field on the front screen allows a system administrator access to set up all the default settings for the programs, time and date settings and to change and remove passwords.

Access to the 'Configuration' set up is password protected.

To enter configuration, use the alpha keys to type in 'pad'

Press "Enter"

Programs [0]

Information

Configuration [ , to select ]

Block 20x0.5ml Block s/n 139160-2 Unit s/n GP0000-01 S/w version 14.zz H/w version 01 Comms address 1 Unit cycles 624 Block cycles 69

DONNA

Heated Lid on Status ramping [ to exit ]

Programs [0] Information

Configuration

[ , to select ]

[ to exit ]

Program defaults

Settings

There are a number of options which can be changed; these are listed here.

Use the "Up Arrow" and "Down Arrow" keys to select then press "Enter" to access any of the options. Each option is discussed in detail below.

Highlight 'Program defaults' then press "Enter"

The complete list of program defaults is given here. These are the values that all new programs will take when you start to create a new program.

To edit any line use the "Up Arrow" or "Down Arrow" keys to select the line you wish to change.

Press "Enter" then change the value using the numerical keys. Press "Enter" to accept the new value.

To edit a field with on/off settings, first select the field and press "Enter"

Use the "Up Arrow" and "Down Arrow" keys to toggle between the settings. Press "Enter" to accept.

When you have set all the defaults, press the "Enter" key to go back to the Configuration menu.

Note: altering the default values does not change existing programs.

Highlight 'Settings' then press "Enter"

Program defaults Settings Passwords Controller reset

Program defaults

Settings Passwords Controller reset

Program defaults

Settings

Passwords Controller reset

Heated Lid 105°C

Preheat Lid off Pause off

------------------------In denat 94°C 5m00 Hot Start off

------------------------Final Extn 72.0°C 5m00 Final hold 10.0°C [ , to select ]

The complete list of settings is given here.

Auto restart "on" will allow the unit to restart from where it left off should there be a power failure and the power returns.

The "Comms address" refers to the location of a unit in a daisy chain and must be unique if there are several units linked to each other.

The optimum "Coms speed" is set at 4800. When using the Gensoft control software, ensure all cyclers are set to the same value.

Edit any line as described for 'Program defaults' above. Press "Enter" to accept the new value.

When you have finished, press the "Enter" key to go back to the 'Configuration' menu.

The 'Passwords' function allows the administrator to change the 'Admin password' (which allows access to Configuration) and also to delete any passwords associated with programs.

Highlight 'Passwords' then press "Enter"

The screen will appear as opposite.

We suggest that you change the 'Admin password' at this stage in order to protect the configuration settings.

Use the "Up Arrow" key to highlight 'Admin passwd

'Press "Enter" and type in the new password. Repeat to confirm and accept.

The screen will go back to the 'Configuration' menu.

To delete a password associated with a program, use the "Up Arrow" and "Down Arrow" keys to highlight the program.

Press "Enter"

Press "Enter" again to delete the password.

Passwords

Set date xx/xx/xx Set time xx:xx Date format dmy Auto restart on Beeper on Coms address 1 Coms speed 4800 [ , to select ]

Program defaults Settings

Passwords

Controller reset

* * Admin passwd * *

DONNA

GEORGE [ , to select ]

* * Admin passwd * *

DONNA

GEORGE

[ DEL PASSWORD? ]

ADMIN PASSWD Password Confirm [ to accept ]

[ Yes or No ]

Controller reset

The 'Controller reset' will delete all saved programs from the unit.

Highlight 'Controller reset' then press "Enter"

To delete all the programs, press "Enter"

RUNNING THE TC-3000 & TC-3000X FROM A PC

The thermal cyclers can be run from a PC using Bibby Scientific Ltd's Gensoft software. This is available to download from the Techne web-site at www.techne.com. Instructions for using Gensoft are included in a separate manual.

Connecting one unit to the computer using an RS 232 cable

If you are driving one TC-3000 unit you will need part number FGEN232 which includes a 9-pin female to female, null modem, RS232, fully screened cable, Gensoft on disk and an instruction sheet. To connect a single TC-3000X unit, connect the unit to the PC using the USB cable, part number 6205046.

Program defaults Settings Passwords

Controller reset

CONTROLLER RESET This will delete all programs [ SURE ? ]

[ Yes or No ]

TC-3000 TC-3000X

Connecting several TC-3000 units to the computer using the daisy chain cables

If you are driving two or more units then you will need part number FGEN485D, a 230V UK power pack set, or FGEN485E, a 230V European power pack set, or FGEN485P, a 120/100V USA power pack set. A power pack set includes: the connection to a computer (which also converts to RS485), a power pack, the necessary cables, a termination box and the software on disk with an instruction sheet. You will also need one or more of the following:

FGENTWO A two unit set which includes two connectors for the cyclers and a cable to

interconnect them.

FGENFIVE A five unit set which includes five connectors for the cyclers and four cables to

interconnect them.

FGENTEN A ten unit set which includes ten connectors for the cyclers and nine cables to

interconnect them.

FGENONE A one unit set for extending the daisy chain further which includes one connector for

the cycler and one cable for connecting it to the daisy chain.

Notice from the diagram that the cable connections to the cyclers are alternately bottom to top on the connector.

Computer

Adaptor (if necessary)

Connector

Te rminator

Cables

Power

pack

Power

cable

Computer connector/

RS485 converter

Connecting several TC-3000X units to the PC.

If you are driving two or more units then you will need a USB cable for each unit, part number 6205046 plus a hub, which will connect multiple units together.

Computer

USB cable

USB hub

USB cables

NOTES ON TRANSFERRING PROTOCOLS FROM OTHER THERMAL CYCLERS

The TC-3000 and TC-3000X can heat up to 3.6°C/s and provides the highest levels of temperature uniformity. Many slower machines have historically begun the countdown on hold times before the set temperature is reached in order to avoid any overshooting in sample temperature. The slower the heating rate the more significant the loss in hold time, i.e. if a 30 second hold time is set at 55°C then perhaps 5 to 10s will be lost in between the start of the time countdown and actually reaching the set temperature. The overall effect of this method of control is that your sample, if cooling from a higher temperature, is subjected to an environment with an elevated temperature compared to the target temperature for a significant part of the hold time.

The TC-3000 and TC-3000X provide the user with 'what-you-program-is what-you-get'. Due to the rapid and responsive nature of the Peltier heating system, the time between the timer countdown initiating and actually reaching the set temperature has been reduced to as little as 1s. This ensures that the sample is only subjected to the exact temperature required.

Taking the above into consideration it must therefore be noted that care must be taken when reoptimizing protocols from a slower machine.

Trouble Shooting

1. The optimum annealing temperature may in fact be higher than the set temperature used in the slower machine. Try increasing it by 1 or 2°C.

2. The hold time can be reduced once the optimum temperature has been found. Try reducing it by 5 to 10s.

USER MAINTENANCE

CLEANING YOUR TC-3000 OR TC-3000X

Before cleaning your unit, disconnect from the power supply and allow it to cool to below 50°C.

CLEANING YOUR BLOCK

The heating/cooling block, including wells and flat surfaces, should be cleaned regularly to ensure optimum heat transfer to the samples. Always clean the block if there has been a spillage. Use a cloth or cotton buds dipped in a fresh, 50:50 water/isopropanol solution and make sure that no deposits are left in the wells.

In the case of radioactive spillages the heater block can be removed from the unit for more detailed cleaning. Bibby Scientific Ltd recommends that you use a proprietary cleaning agent and follow the manufacturer’s instructions. The heating/cooling block is made of aluminium, therefore an agent such as Neutracon (from Decon Labs Ltd.), suitable for nonferrous metals should be used. However, remember that other parts of the unit are made of ferrous materials and may be damaged by spillage onto them.

HEATER BLOCK REMOVAL

Removing the block may expose the possibility of electric shock; disconnect the unit from the

mains and allow to cool below 50ºC before continuing. Remove the four screws on the underside of the unit and drop the block/fan assembly down. Unplug the assembly from the wiring and remove the assembly from the unit. Refitting the block/fan assembly is the reverse procedure; be careful of the wires as you refit the block.

CLEANING YOUR CASE

The outer case of the TC-3000 and TC-3000X can be cleaned with a cloth dipped in water or ethanol (50:50 methanol/water or 99:1 water/formaldehyde can also be used). No part of the case or cover should be immersed in the solvents. Do not use aggressive solvents such as acetone, or abrasive cleaners. Before using any cleaning or decontamination method except those recommended here, the responsible person should check with Bibby Scientific Ltd that the proposed method will not damage the equipment.

Four screws which hold the block/fan assembly in place

Underside of the TC-3000 (TC-3000X is identical)

SOME FREQUENTLY ASKED QUESTIONS

Q1 What is the 'Pause' function at the start of the program used for?

A1 Some users prefer to preheat the heated lid before placing the samples into the unit. The pause

feature is used to stop the unit after the 4 minute heated lid preheat step. It will also sound an audible alarm indicating that the machine is ready for the sample tubes or plate to be added. Pressing the "Pause" key followed by the "Enter" key will commence the remaining program.

Q2 Why do you have to press some keys twice to make the unit do what you want it to do?

A2 With many of the program features there is a fail-safe function which asks if you are sure you want

to perform a particular action. You are required to press one key to select a function followed by a second to accept it.

Q3 What is 'Hot Start'?

A3 A "hot start" is used for reducing non-specific product formation during the first cycle of

amplification. The 'Hot Start' programming step is used to pause the instrument at a specific temperature, typically around 70ºC, after the initial template denaturation. The reason is to allow the manual addition of unmodified Taq DNA polymerase which may loose activity if added during the initial 5min denaturation. Heat-activated Taq or Hot Start enzymes do not require this step.

Q4 What is the incremental function?

A4 Incremental timing and temperature are used to increase or decrease either the time or temperature

incrementally over the number of cycles in a stage.

Incrementation of extension time is used with 'Long PCR' which is when large template fragments are to be amplified (e.g. 27kb lambda DNA, 40kb genomic DNA).

Decremental temperature is used for protocols such as 'Touchdown PCR' where one starts with a high annealing temperature in the first cycle and gradually decreases the temperature over the number of cycles in the stage. This ensures that only the specific product is amplified.

Q5 The heat-up ramp-rate in the specification is 3.6°C/second yet I can only manually set it to

2.0°C/second. Why is this?

A5 If you set the ramp-rate to max the block will heat at 3.6°C/second the unit sets its own control

parameters. If you program a ramp-rate the control parameters reduce the ramp-rate. This is a feature of the software and not the actual ramp-rate.

If you require further technical or applicational assistance please contact Bibby Scientific Ltd at:

technehelp@bibby-scientific.com.

For servicing information please contact: service@bibby-scientific.com.

We are continually striving to improve our thermal cyclers and software. If you have any comments and suggestions on how we can do things better please send them to us at:

techne@bibby-scientific.com

ADDITIONAL INFORMATION

Brief fault finding notes and a list of replacement parts are given in this section.

Note that this equipment should only be dismantled by properly trained personnel. Removing the outer cover exposes potentially lethal mains voltages. There are no user serviceable parts within this equipment.

FAULT FINDING

Should you have any problems with your unit which cannot be easily remedied, you should contact your supplier and return the unit if necessary. Please include details of the fault observed and remember to return the unit in its original packing. Bibby Scientific Ltd accepts no responsibility for damage to units which are not properly packed for shipping: if in doubt, contact your supplier, giving the full serial number of the unit and software version number (shown when the unit is first switched on).

FUSES

If neither the power light nor display on the front panel is lit, one of the two fuses may have blown. Check that there is no external cause, such as a faulty plug or lead. Check both fuses and replace the faulty fuse with a new one of the correct value (fuse values are given on the label next to the power inlet). Note that fuses should only be replaced by a qualified electrician.

The holder for the two fuses is built into the mains input socket. First remove the power cable and then gently prise the fuse drawer open with a flat-bladed screwdriver or similar tool.

Each fuse can be removed by using the screwdriver as a lever.

Exchange both fuses in the fuse holder for a working fuse of the correct value. Finally, replace the fuse drawer in the fuse compartment and push the drawer shut.

Fuses which blow repeatedly indicate a serious fault and you should return the unit to your supplier for repair.

THE HEATED LID OVER-TEMPERATURE CUT-OUT

The heated lid is fitted with an independent circuit to protect it from overheating. In the unlikely event of an over-temperature problem with the lid, the unit is fitted with a thermal fuse which will remove power to the heater plate should the maximum temperature be exceeded. If this fault occurs, contact your local supplier for repair.

INSULATION TESTING

This equipment is fitted with RFI suppression circuitry. Any check of the electrical insulation by means of high voltage dielectric testing (for example as in BS EN 61010-1) must be carried out using only a DC voltage.

This unit contains semiconductor components which may be damaged by electric field effects.

INTERCHANGEABLE BLOCKS

The block can be removed and replaced by another of the same sort or of a different sort. The software will register the change and set up the unit for the new block.

Remove the four screws on the underside of the unit and drop the block/fan assembly down. Unplug the assembly from the wiring and remove the assembly from the unit. Refitting the block/fan assembly is the reverse procedure; be careful of the wires as you refit the block.

Underside of the TC-3000 (TC-3000X is identical )

ACCESSORIES

The following accessories can be obtained from Bibby Scientific Ltd or your Bibby Scientific Ltd dealer:

Item No Description Quantity

FTC3/02/B 0.2ml Tube Block for TC-3000 1

FTC3/05/B 0.5ml Tube Block for TC-3000 1

FTUB02TW 0.2ml Micro tubes Pack of 1000

FTUB05TW 0.5ml Micro tubes thin wall Pack of 1000

FTC3X/02/B 0.2ml Tube block for TC-3000X 1

FTC3X/05/B 0.5ml Tube block for TC-3000X 1

FSTRIP2 Strips of 0.2ml tubes for use with flat or domed caps Pack of 125 strips

FSTRIP3 Strips of 0.2ml tubes, each tube has individual flat cap Pack of 120 strips

FSTRIP4 Strips of 0.2ml tubes with attached, hinged strip of domed caps Pack of 125 strips

F8CAP2 Strips of 8 domed caps for use with FSTRIP2 Pack of 125 strips

F8CAP3 Strips of 8 flat caps for use with FSTRIP2 Pack of 125 strips

FCOOL Mini cooler. Suitable for all 0.2ml tubes, strip tubes & plates 1

F24PLNS 24 well plate, non-skirted 40 plates

F48PLNS 48 well plate, non-skirted 20 plates

F24MAT 24 well sealing mat (silicone) for use with F24PLNS 10 mats

F48MAT 48 well sealing mat (silicone) for use with F48PLNS 10 mats

Four screws which hold the block/fan assembly in place

REPLACEMENT PARTS

The following replacement parts can be obtained from Bibby Scientific Ltd or your Bibby Scientific Ltd dealer:

Item No Description Quantity required

FCABRTUK Mains cable and plug, 230V UK 1

FCABRTEU Mains cable and Schuko plug, 230V Europe 1

FCABRTUS Mains cable and 3-pin plug, 100V/120V US type 1

6501231 Fuse T2.5A (TC-3000 230V units) 2

6500129 Fuse T4A (TC-3000 120/100V units) 2

6500342 Fuse T6.3A (TC-3000X) 2

6106196 Heated lid mat (TC-3000X) 1

Bibby Scientific Ltd

Beacon Road Stone Staffordshire ST15 0SA United Kingdom Tel: +44 (0)1785 812121 Fax: +44 (0)1785 813748 email: techne@bibby-scientific.com www.bibby-scientific.com

Bibby Scientific France SAS

ZI du Rocher Vert - BP 79 77793 Nemours Cedex France Tel: +33 1 64 45 13 13 Fax: +33 1 64 45 13 00 e-mail: bsf@bibby-scientific.fr www.bibby-scientific.com

Bibby Scientific Italia Srl

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Techne TC-3000X User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual