Techne PrimeQ Frequently Asked Questions
FrequentlyAskedQuestions
Customer Question Regarding PrimeQ Features Recommended Response
• Yes
Multiplexing
Quantification and Relative Quantification
Analysis Method
• PrimeQ can multiplex up to 4 dyes
• Interchangeable excitation/emission filters are
convenient allowing the investigator multiple multiplex
options
• Yes
• Quansoft® will allow you to use Fit Point Analysis and
First Derivative Maximum to determine Cq and
concentration values.
• Fit Point analysis is not a common method found on
most of the competitor software.
Reverse Transcriptase PCR
Gradient
Consumables Flexibility
Reagent Flexibility
Being able to run the software simultaneously
while experiments are running
• Yes, heated lid can be adjusted every stage to
accommodate reverse transcriptase environmental
needs.
• No
• Gradient is not necessary for a qPCR protocol because
you are able to analyze your product in real-time during
the run and after the run.
• You can also perform a dissociation curve to determine
a primer’s or a target sequence’s melting temperature.
• Yes
• You can use consumables from any manufacturer with
PrimeQ.
• Plates and tubes should be low profile.
• Seals should be optically clear.
• Yes
• PrimeQ can accommodate reagents from any
chemical/reagents manufacturer.
• Any future dyes can be accommodated with the ease
that interchangeable filters provide.
• Yes
• While running an experiment, the end user can freely
navigate through Quansoft®.
• They can fill in any Plate Layout information they didn’t
prior to the run. Thus, the end user can pop in the plate
and start the program.
Availability of Raw Data.
Software Licenses
.
Analyzing data from multiple PCs
• Yes
• PrimeQ uses a PMT detector which allows for the
population of raw data.
• No
• The end user does not need a new software license
every time he or she wants to download Quansoft® on
a new PC.
• No extra software purchases are necessary.
• Yes
• You do not need to purchase additional copies of
software for additional PCs
• You transfer any type of Quansoft® file from one PC to
another via USB stick, email, CD, etc.
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