Techne PrimeQ Instruction Manual

Techne® PrimeQ
Real-time PCR System

Software

®

Multiplex capability

Open chemistry

White LED Light source

PMT Detector

Optically heated lid

Wide Dynamic Range

High Sensitivity

QuanSoft

2 year warranty

Introducing the PrimeQ

The PrimeQ instrument has been designed with

the advantage of having an open architecture

and chemistry format that allows the end

user full flexibility of the qPCR methods and

research they wish to pursue.

Range of excitation/emission wavelengths 470-710nm

Photon
Counting PMT

Emission
filter

Dichroic mirror

White light LED

Excitation filter

Fiber optic bundle

= Excitation light

= Emitted light

Excitation light
to the sample

Emitted light collected
from the sample

Single White LED Light Source

• Ensures consistent power output to each well

compared to tungsten halogen lamps or other

multiple LED systems.

• Provides a long life span unlike tungsten halogen

lamps that require frequent replacement.

PMT (Photomultiplier tube) Detector

• PMT is designed for accurate detection of photons

compare to CCD cameras which are just an imaging

system.

• PMT provides raw data for analysis that can be

manipulated directly whereas CCD uses image

comparisons interpreted by internal algorithms.

Flexible scanning mechanism

• Allows both partial and full plate reads.

• High accuracy ensures no cross talk between well

reads

• Full plate read in 20 seconds per filter.

Multiplex capabilities

• 4 paired excitation and emission filters housed in an

individual cartridge system

• Open system to preferred chemistry and not locked

in to factory calibrated dyes.