Techne® PrimeQ
Real-time PCR System
Software
®
Multiplex capability
Open chemistry
White LED Light source
PMT Detector
Optically heated lid
Wide Dynamic Range
High Sensitivity
QuanSoft
2 year warranty
Introducing the PrimeQ
The PrimeQ instrument has been designed with
the advantage of having an open architecture
and chemistry format that allows the end
user full flexibility of the qPCR methods and
research they wish to pursue.
Range of excitation/emission wavelengths 470-710nm
Photon
Counting PMT
Emission
filter
Dichroic mirror
White light LED
Excitation filter
Fiber optic bundle
= Excitation light
= Emitted light
Excitation light
to the sample
Emitted light collected
from the sample
Single White LED Light Source
• Ensures consistent power output to each well
compared to tungsten halogen lamps or other
multiple LED systems.
• Provides a long life span unlike tungsten halogen
lamps that require frequent replacement.
PMT (Photomultiplier tube) Detector
• PMT is designed for accurate detection of photons
compare to CCD cameras which are just an imaging
system.
• PMT provides raw data for analysis that can be
manipulated directly whereas CCD uses image
comparisons interpreted by internal algorithms.
Flexible scanning mechanism
• Allows both partial and full plate reads.
• High accuracy ensures no cross talk between well
reads
• Full plate read in 20 seconds per filter.
Multiplex capabilities
• 4 paired excitation and emission filters housed in an
individual cartridge system
• Open system to preferred chemistry and not locked
in to factory calibrated dyes.
Introducing QuanSoft
Accompanying PrimeQ® is our unique, intuitive software
®
Quansoft
Quansoft
and analysed with ease.
. Employing four user-friendly editor functions
®
enables any real-time experiment to be created
®
1. Plate layout editor
Define your PCR plate in seconds with colour coded sample
identifications.
2. Program editor
Individual cycles and steps as well as a ramp read can be
added quickly to build up and display the thermal program
and read points.
Crossing line
Noise threshold
Quantification analysis
Using intercalating dyes or probes PrimeQ provides a
wide dynamic range and high sensitivity
• Linearity of at least 9 orders of magnitude
• Detecting down to a single copy template or to
achieve absolute quantification of 1nM fluorescein
in a volume of 20µl.
3. Experiment editor
Combine thermal cycling programs, plate layouts and
analysis methods to generate a new complete experiment.
4. Results editor
Choose your analysis method and manipulate your raw
data before packaging the results in a customised report.
Dissociation analysis
Provides the user confident reporting in genotyping
experiments and in product verification analysis.
End point analysis
• Plus/minus scoring
o Determine the presence of the nucleic acid in
the sample.
• Allelic discrimination
o Yes/No answer to the question “is my target
present in the sample?”
Features
Block format 96 x 0.2ml
Block specification 8 x Peltier block employing quad-circuit technology to enhance performance
Block uniformity at 50°:C < ±:0.3°C
Maximum ramp rate Up to 2.2°C
Temperature range 4 to 98°C
Sample volume 15 to 50ul
Heated lid Adjustable between 100 and 115°C in 1°C increments, or off
Maximum sample evaporation Less than 15% volume loss from any well using the plate sealing recommended by Techne
PC connectivity USB, OS Win XP SP3 or higher and Win 7 (one unit per PC)
Plate format Low profile 96-well plate (skirted or non-skirted)
Excitation source Solid state white light source
Detector Photon counting photomultiplier tube
Multiplex dye detection Up to 4 dyes per reaction tube
User selected filters Maximum of 4 paired excitation/emission filter cartridge systems suitable with currently
used dyes*
Fluorescence excitation range 470-650nm (standard filters are centred at 485, 530, 580 and 640nm)
Fluorescence detection range 500-710nm (standard filters are centred at 520, 560, 615 and 685nm)
Dynamic range At least 9 orders of magnitude of target DNA concentration
Sensitivity (detection threshold) 1nM fluorescein in a 20ul sample
* Custom filter cartridge systems available upon request
Ordering information
Product Code Description
PRIMEQ PrimeQ real-time PCR System
FC01 FAM multiplex, 460nm excitation wavelength, 500nm emission wavelength
FC02 Green, FAM/SYBR®, 485nm excitation wavelength, 520nm emission wavelength
FC03 Yellow, HEX, 530nm excitation wavelength, 560nm emission wavelength
FC04 Red, ROX 580nm excitation wavelength, 615nm emission wavelength
FC05 Blue, Cy5, 640nm excitation wavelength, 685nm emission wavelength
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Bibby Scientic USA (Techne Inc.)
3 Terri Lane, Suite 10, Burlington, NJ 08016, USA
Toll-free: 1.800.225.9243
Fax: 1.609.589.2571
Email: info@techneusa.com
www.techneusa.com
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