Prime Pro 48
Real Time PCR System
High uniformity ±0.1ºC
Adaptive LED control
Fast ramping 5.5ºC/sec
40 cycles in 40 minutes
High resolution melt
MIQE guidelines compliant
Accurately quantify NGS libraries
400 qPCR detection kits
Validated sample 5 to 20μl
Introducing Prime Pro
The Prime Pro 48 real time PCR system is a high specification, economically priced real
time thermal cycler that accommodates a unique 48-well polypropylene PCR plate
utilising the same geometry as standard 384-well plates, but only 1/8 of the size.
This enables users to dramatically reduce the qPCR reagent volumes compared to
traditional 96-well instruments, saving users precious sample, whilst still producing
a strong fluorescence signal. Minimizing the plate size also significantly improves
thermal uniformity. A minimum volume of 5μl is validated, resulting in a more efficient
use of expensive and ‘hard to acquire‘ template DNA samples.
Key features
High speed
• Ramp rate average 5.5°C/sec. Fast heating and cooling.
• 40 cycles in 40 minutes (under 20 minutes optimised)
with standard chemistry.
• Process up to 40% more samples per hour than a
traditional 96-well instrument.
• MIQE guidelines compliant software.
Reagents
PC not
supplied
Thermal system
• High resolution melt (HRM) capability.
• Patented thermal system. Hollow fluid-filled silver block
anodised with gold.
• Unparalleled market-leading uniformity ±0.1°C at 95°C.
• Up to 5x greater uniformity than any other block-based
qPCR instrument.
Advanced optical technology
• Techne supply 400 probe based qPCR detection kits.
• Kit components are lyophilised for ambient shipping and
storage.
• Prime Pro 48 also accepts all popular brands of reagents.
• 4 colours for easy multiplexing.
• Patented optical system utilised Adaptive LED Control.
• Automatically normalises fluorescence. Never saturate
the detector. Eliminate cross talk.
• Always reads all 48 wells, with all 4 filters at every cycle.
Prime Pro workflow
ProDock
1. Load samples into 48 well plate using backlit loading block.
2. Insert plate into Prime Pro 48.
3. Run a MIQE compliant experiment via ProControl software.
4. Analyse results in ProStudy software.
5. Generate a full report automatically.
The ergonomic plate loading dock allow for easy
loading of the plate and is backlit to allow the user
to easily visualise sample loading.
• Base with LED backlight
• Plate adapter fits standard centrifuge rotors
• Includes plate sealing tool
Advanced optical technology
Intuitive icons lead researchers
through setup, run and analysis
quickly and easily
The Pro 48 Real-time system offers the qPCR capabilities of larger instruments in a compact, accurate footprint. Innovative features
include a precise thermal system for unrivalled temperature control, an advanced optical system for highly sensitive fluorescence
detection, a 48-well plate for flexible sample throughput, and intuitive, icon-driven software for error-free instrument operation.
Sensitive optical system
delivers precise detection for
a range of fluorophores
Convenient 48-well format
meets the throughput needs
of most researchers
Unique thermal system provides
unmatched temperature control
for the most accurate results
Prime Pro 48 thermal system
qPCR specificity and efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy,
temperature must remain uniform across the entire heat block, ensuring that all samples are processed equally. The unique thermal block
design of Pro 48 achieves this with a unique heating and cooling system that provides accurate ±0.1ºC temperature control and quickly
cycles from one temperature to the next.
Hollow pure silver block filled with
conductive fluid, gold electroplated
Peak paddles on
titanium bearings
To achieve true temperature control the Pro 48 thermal system
consists of a precisely electroformed 48-well hollow silver block
containing conductive fluid. The block is heated and cooled by a
single peltier device, with an agitator assembly consisting of two
paddles driven by electromagnetic motors. During PCR cycling,
the paddles move rapidly, circulating the fluid across the 48 wells,
allowing the block to achieve high ramp rates, thus reducing the
overall experiment time.
Design developed at the Caltech Laboratories of
Nobel Laureate David Baltimore
6000 RPM drive motors
This unique design delivers industry leading thermal stability
of ±0.1°C virtually eliminating thermal non-uniformity and
preventing edge effect. The result is higher qPCR performance,
tighter Cq, greater PCR efficiency, higher R
perform demanding HRM applications.
Fast, uniform temperature control is important because accurate
dwell temperatures ensure primers bind most efficiently and
polymerase enzymes work optimally, generating the maximum
yield of target DNA. The hermetically sealed hollow block
contains a conductive fluid and two opposing agitators driven
by electromagnetic motors. During PCR cycling, these agitators
rapidly circulate fluid throughout the hollow block, transferring
heat from the peltier quickly and evenly.
Single peltier element
2
and the ability to