Techne Prime Pro 48 Instruction Manual

Prime Pro 48
Real Time PCR System

High uniformity ±0.1ºC

Adaptive LED control

Fast ramping 5.5ºC/sec

40 cycles in 40 minutes

High resolution melt

MIQE guidelines compliant

Accurately quantify NGS libraries

400 qPCR detection kits

Validated sample 5 to 20μl

Introducing Prime Pro

The Prime Pro 48 real time PCR system is a high specification, economically priced real

time thermal cycler that accommodates a unique 48-well polypropylene PCR plate

utilising the same geometry as standard 384-well plates, but only 1/8 of the size.

This enables users to dramatically reduce the qPCR reagent volumes compared to

traditional 96-well instruments, saving users precious sample, whilst still producing

a strong fluorescence signal. Minimizing the plate size also significantly improves

thermal uniformity. A minimum volume of 5μl is validated, resulting in a more efficient

use of expensive and ‘hard to acquire‘ template DNA samples.

Key features

High speed

• Ramp rate average 5.5°C/sec. Fast heating and cooling.

• 40 cycles in 40 minutes (under 20 minutes optimised)

with standard chemistry.

• Process up to 40% more samples per hour than a

traditional 96-well instrument.

• MIQE guidelines compliant software.

Reagents

PC not
supplied

Thermal system

• High resolution melt (HRM) capability.

• Patented thermal system. Hollow fluid-filled silver block

anodised with gold.

• Unparalleled market-leading uniformity ±0.1°C at 95°C.

• Up to 5x greater uniformity than any other block-based

qPCR instrument.

Advanced optical technology

• Techne supply 400 probe based qPCR detection kits.

• Kit components are lyophilised for ambient shipping and

storage.

• Prime Pro 48 also accepts all popular brands of reagents.

• 4 colours for easy multiplexing.

• Patented optical system utilised Adaptive LED Control.

• Automatically normalises fluorescence. Never saturate

the detector. Eliminate cross talk.

• Always reads all 48 wells, with all 4 filters at every cycle.

Prime Pro workflow

ProDock

1. Load samples into 48 well plate using backlit loading block.

2. Insert plate into Prime Pro 48.

3. Run a MIQE compliant experiment via ProControl software.

4. Analyse results in ProStudy software.

5. Generate a full report automatically.

The ergonomic plate loading dock allow for easy

loading of the plate and is backlit to allow the user

to easily visualise sample loading.

• Base with LED backlight

• Plate adapter fits standard centrifuge rotors

• Includes plate sealing tool

Advanced optical technology

Intuitive icons lead researchers
through setup, run and analysis
quickly and easily

The Pro 48 Real-time system offers the qPCR capabilities of larger instruments in a compact, accurate footprint. Innovative features

include a precise thermal system for unrivalled temperature control, an advanced optical system for highly sensitive fluorescence

detection, a 48-well plate for flexible sample throughput, and intuitive, icon-driven software for error-free instrument operation.

Sensitive optical system
delivers precise detection for
a range of fluorophores

Convenient 48-well format
meets the throughput needs
of most researchers

Unique thermal system provides
unmatched temperature control
for the most accurate results

Prime Pro 48 thermal system

qPCR specificity and efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy,

temperature must remain uniform across the entire heat block, ensuring that all samples are processed equally. The unique thermal block

design of Pro 48 achieves this with a unique heating and cooling system that provides accurate ±0.1ºC temperature control and quickly

cycles from one temperature to the next.

Hollow pure silver block filled with
conductive fluid, gold electroplated

Peak paddles on
titanium bearings

To achieve true temperature control the Pro 48 thermal system

consists of a precisely electroformed 48-well hollow silver block

containing conductive fluid. The block is heated and cooled by a

single peltier device, with an agitator assembly consisting of two

paddles driven by electromagnetic motors. During PCR cycling,

the paddles move rapidly, circulating the fluid across the 48 wells,

allowing the block to achieve high ramp rates, thus reducing the

overall experiment time.

Design developed at the Caltech Laboratories of

Nobel Laureate David Baltimore

6000 RPM drive motors

This unique design delivers industry leading thermal stability

of ±0.1°C virtually eliminating thermal non-uniformity and

preventing edge effect. The result is higher qPCR performance,

tighter Cq, greater PCR efficiency, higher R

perform demanding HRM applications.

Fast, uniform temperature control is important because accurate

dwell temperatures ensure primers bind most efficiently and

polymerase enzymes work optimally, generating the maximum

yield of target DNA. The hermetically sealed hollow block

contains a conductive fluid and two opposing agitators driven

by electromagnetic motors. During PCR cycling, these agitators

rapidly circulate fluid throughout the hollow block, transferring

heat from the peltier quickly and evenly.

Single peltier element

2

and the ability to

4 band pass filters

camera

(upgradable to 5)

48 blue LED
array

48 green
LED array

Thermal system

The Prime Pro 48 Real Time PCR system contains an advanced high-performance optical system that delivers precise and sensitive

®

CCD

fluorescence detection, facilitating all 4 colour multiplex applications. The system is provided factory-calibrated for use with SYBR

TM

, VICTM, ROXTM, Cy®5 and can also be used with any qPCR fluorophores overlapping with these calibrated dyes. For excitation, two

HEX

panels of 48 fixed LEDs provide excitation energy of distinct spectra, enabling broad range excitation. Each of the 48 LEDs illuminates a

specific well location, eliminating the optical distortion created by most stationary optical systems. HRM analysis protocols are supported

by continuous data acquisition during the melt for increased data collection and reduced run times, HRM of a full plate is less than 10

minutes. The user can change the plate setup and perform data analysis after the run is complete.

, FAMTM,

Adaptive LED control (ALC)

ALC normalises variation in fluorescence across all wells at each cycle of a run and provides specific tuning for each LED following each

PCR cycle.

• Speeds up data collection because only 1 image is required

to cover the full sample dynamic range.

• Expands the linear range of detection by reducing LED

exposure to high emission wells and preventing

premature detector saturation.

• LED adjustment following each amplification allows Prime Pro

48 to reduce exposure time as signal intensity increases,

maximising the linear range of emission detection.

• Minimise optical artefacts by keeping each well

brightness similar to the CCD. This reduces the

influence of a highly fluorescing sample on adjacent

wells and avoids ‘blooming’, no artificats from

adjacent wells.

• Maximise sensitivity by permitting an appropriate

exposure level for each well, by dye, rather than

compromise with a universal setting.

Pre-scan

LED Array

1 2 3 4 5 6 7 8
A
B
C
D
E
F

  

LED excitation time

A1
A2
A3
A4
A5
A6
A7
A8
B1
B2
B3
B4
B5
B6
B7
B8
(etc)

exposure duration (milliseconds)

 

1 2 3 4 5 6 7 8
A
B
C
D
E
F

Alternative plate-based qPCR systems

excite all samples with equivalent amounts

of energy, leading to large differences in

emission energy between wells. This leads

to crosstalk as a result of light emission

from wells with strong signals affecting

adjacent wells with weaker signals.

Applications

The Prime Pro 48 is a high performance instrument for demanding applications.

Absolute & Relative Quantitation

• Absolute quantification standard curves with

efficiency calculation.

• Relative quantification using the ΔΔCq method with

support for multiple reference gene normalization.

• Relative gene expression with efficiency control.

• Next Generation Sequencing (NGS) library quantification.

• Copy Number Variation: insertions, deletions, inversions.

• RNA Characterisation: siRNA and miRNA.

• Viral load.

HRM
Differential
plot

Genotyping and High resolution melting

• Allelic Discrimination.

• DNA Methylation.

• SNP Verification.

• Microbiology: pathogen detection and identification.

High resolution melting (HRM)

• Genotyping, methylation studies and mutation

screening.

Absolute
Quantification
standard curve

Relative gene
expression

Library quantification

• qPCR is the most precise method for quantifying

libraries prior to cluster generation.

• Works at concentrations below the detection

threshold of conventional spectrophotometric

methods.

Data

• Validation of array and sequencing quality data.

Designed for:

• Academic, Government and Corporate laboratories.

• Human research, AgriGenomics, Translational, Forensics

and many more markets.

• Probe, DNA Binding Dye and HRM chemistries.

• Gene expression, Viral detection, Sequencing Library

Preparation, Genotyping and Mutation Screening

applications.

• Pro 48 is for research use only – not suitable for

human or veterinary clinical diagnostics.

Discrimination
of 2-fold
differences

Allelic
discrimination

NGS library
quantification

Rapid cycling

The Pro 48 has rapid heating and cooling rates. Average ramp rates

of 5.5°C/sec reduce overall PCR run times. The typical run time for

a 40 cycle PCR protocol is under 20 minutes when optimised.

Pro 48 hollow silver fast
block, gold plated

High throughput

Pro 48 thermal control is superior to traditional 96-well peltier systems where thermal efficiency decreases as distance from the peltier

increases. With Pro 48, faster and more precise cycling increases sample throughput because the need for multiple replicates is reduced

by increased uniformity across the block. Pro 48 well-to-well, plate-to-plate and instrument-to-instrument results are as consistent as

running a single experiment. To take advantage of this, Pro 48 has a function in the software where multiple experiments are combined.

High uniformity eliminates the need for triplicates which reduces reagent running costs and ensures the entire plate of data is valuable.

Prime Pro 48 wells

HIGH uniformity - Run duplicates

±0.1ºC uniformity means Prime Pro 48
requires fewer replicates than a conventional
96 well system.

The Prime Pro 48 is capable of running 40
cycles in 40 minutes.

Conventional 96 wells

LOW uniformity - Run triplicates

Results from multiple instruments can be combined together

Also available from Techne

Techne® has developed a comprehensive new range of

thermal cyclers around its belief that all users deserve PCR

without compromise. Our design philosophy has focused on

delivering an enhanced user experience on all models.

®

24 samples

Run time 40 minutes

32 samples

Run time 1 hour 20 minutes

36 samples

per hour

Fewer replicates and faster cycling
allows Prime Pro 48 to process more
samples than a standard 96 well
system.

24 samples

per hour

• USB software upgrade and program transfer

• Total reliability with 4 year warranty

• Touchscreen interface on all models

• Unique gradient upgrade function

• High performance cycling block

• Exceptional value for money

PC not
supplied

Software

Easy to use software that integrates user control, real-time data collection, and advanced data analysis.

Pro software.

• User-installable, intuitive, easy to use software that

integrates user control, real-time data collection, and

advanced data analysis.

• Supplied fully featured including HRM capability.

• New software version 5 enables a widened chemistry

library and contains new thermal profiles.

• Facilitates rapid thermal cycling: 40 cycles in 40 minutes.

• Supplied on a USB stick.

ProStudy software.

• Data analysis and Multi-Experiment Analysis.

• Allows higher throughput.

• Expert results to Excel and Powerpoint

• Supplied on a USB stick.

Easy-to-Use Interface

• Pro 48 software uses a unique icon-driven user

interface to simplify experimental design and setup.

• Pre-set thermal profile defaults are provided for the

most commonly used experimental protocols.

• Temperature and time for each protocol step can easily

be changed by click-and-drag action with the mouse.

• Experiment templates can be customized and saved for

future use.

Data Analysis

• With the Pro 48 system and software, data collection

is monitored in real time, allowing researchers to access

run viability immediately.

• The user-friendly data analysis interface also allows

researchers to easily view the amplification plot, melt

curve analysis, and the analysed results, including Cq

2

values, PCR efficiency, R

• Data can be exported into Excel and custom reports

generated directly into PowerPoint or PDF formats.

• High-resolution images can be directly exported in

multiple image formats, ready to use in presentations.

• Conforms to Minimum Information for Publication of

Quantitative Real-Time PCR Experiments (MIQE)

guidelines, making data analysis and submission for

publication review more efficient.

and Y-intercept.

High-Performance Results

• Due to unmatched temperature control and an

optical system designed for individual well monitoring,

the Pro 48 system produces highly accurate, reliable

results with PCR efficiencies between 90% and 110%

2

and R

> 0.99 when using standard optimized assays.

qPCR probe-based detection reagent kits

Every assay is expert designed, involving in-depth literature

review followed by extensive bioinformatics analysis. The kits are

optimised via a strict biochemical validation on synthetic oligos

to ensure optimal PCR performance. Every kit operates with the

same standardised protocol and cycling conditions, which allows

multiple kits to be used on the same plate in the same PCR run.

• Designed and manufactured in the UK.

• Wide range of 400 qPCR detection kits.

• Designed to work with Pro 48 and all other qPCR machines.

• Kits sold with/without Mastermix, 150 tests.

Human Pathogens

Techne supply a comprehensive range of all the commonly tested

infections and diseases. If your test is not available contact Techne

or your local dealer to enquire about getting a custom kit made

for you.

• Respiratory Infections.

• Sexually Transmitted Diseases.

• Herpes Viral Infections.

• Hepatitis Infections.

• Human Papillomavirus.

• Gastrointestinal Infections.

• Vector-Borne Diseases.

• Meningitis.

• Periodontal Infections.

Biothreat detection

Techne supply a small but key list of detection kits, aimed at

detecting some particularly harmful viruses and bacteria

• Japanese Encephalitis virus.

• Slow Bee Paralysis virus.

• African Horse Sickness.

• Taï Forest Ebola virus.

• Sudan Ebola virus.

• Rift Valley Fever virus.

• Reston ebola virus.

• Zaire ebola virus.

• Bacillus anthracis.

• Yersinia enterocolitica.

Food & Water Testing

With ever increasing demands for clarity on the source and type

of products we are consuming, speciation and pathogen detection

kits have been generated to allow users to do exactly that.

• GMO.

• Speciation.

• Allergens.

• Pathogen Contamination.

Veterinary and Agriculture

A wide range of veterinary and agricultural pathogen detection kits,

all offering a potentially significant economic impact.

• Avian.

• Bovine.

• Ovine - Caprine.

• Equine.

• Feline.

• Canine.

• Porcine.

• Piscean.

Prime Pro 48 technical specification

High Resolution Melt Yes

Volume per well Validated for 5 to 20μl

Detection sensitivity 1 copy

Temp uniformity ±0.1˚C

Temperature range 30 to 100˚C

Average ramp rate 5.5˚C/sec

Thermal system Proprietary hollow silver block, Peltier-based system with conductive fluid

Block format 48-well block

Consumables 48-well custom plates and optical adhesive seals

Optical system Dual LED excitation (452–486 nm and 542–582 nm). CCD camera

4 emission filters (505–545nm, 562–596nm, 604–644nm, 665–705nm)

®

Calibrated dyes SYBR

compatible with Pro 48 filters are supported with no additional calibration

required for implementation

Passive reference dyes Use of ROX

Data collection Data collected in all four filters for all wells regardless of plate setup. Plate

setup for data analysis can be altered after run completes. Melt curve

analysis supports continuous data acquisition in a single filter to provide

increased data point collection and reduced run times

PCR cycle time (standard) 40 cycles in less than 40 minutes

PCR cycle time (FAST) 40 cycles in less than 20 minutes

Dynamic range >9 logs

Calibration Not required

Installation Plug and play design. Installed by experienced or novice scientists

Precision Discriminates 5,000 and 10,000 template copies with 99% confidence

, FAMTM, HEXTM, ROXTM, Cy®5. Additional dyes within the wavelength range

TM

is supported, but optional

Warranty 1 year (parts and labour included)

Voltage 120–240 V

Frequency 50-60 Hz

Nominal current draw 5A

Peak power 500W (typical power is 180W)

Software Multiple-licence Pro 48 system software is included at no additional cost.

All chemistries supported. Applications include Absolute Quantification,

Relative Quantification, Allelic Discrimination, High Resolution Melt (HRM)

Dimensions closed (WxDxH) 34.5cm x 31cm x 32cm (13.6 in × 12.2 in × 12.6 inches)

Dimensions open (WxDxH) 34.5cm x 31cm x 37cm (13.6 in × 12.2 in × 14.5 inches)

Weight 13.6 Kg (30 lb)

Ordering information

PRIMEPRO48 Prime Pro 48 Real Time PCR System, includes Dock and Squeegee, EcoControl and EcoStudy software

USB stick, Plates (Pack of 50), Plate seals (Pack of 50), Power and Ethernet Cables.PC not supplied

PROPLATE48 Pack of 50 Real Time PCR Plates

PROSEAL48 Pack of 50 Real Time PCR Plate seals

PRODOCK48 Prime Pro additional loading dock, with 2 centrifuge adapters

Contact

Bibby Scientific - UK (Group HQ)

Beacon Road, Stone, Staffordshire, ST15 0SA,

United Kingdom

T: [phone]

F: [phone]

E: sales@bibby-scientific.com

Bibby Scientific Ltd France

Bâtiment le Deltaparc

Parc Silic Paris Nord 2

7 rue du Canal, BP 55437 Villepinte

95944 ROISSY Charles de Gaulle, France

T: [phone]

F: [phone]

E: contact@bibby-scientific.com

Bibby Scientific - US

3 Terri Lane, Suite 10, Burlington, NJ 08016, USA

T: [phone]

F: [phone]

E: [e-mail]

Bibby Scientific - Middle East

PO Box 27842 Engomi 2433 Nicosia, Cyprus

T: [phone]

F: [phone]

E: sales@bibby-scientific.me

Bibby Scientific Singapore

Prudential Tower, Level 26, 30 Cecil Street,

Singapore 049712

T: [phone]

F: [phone]

E: sales@bibby-scientific.com

Find out more!!!

Please scan the QR/Mobile Tag with your
smartphone for more information

w w w . t e c h n e . c o m

w w w . b i b b y - s c i e n t i f i c . c o m