Techne Prime Elite User Manual

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Prime Elite

OPERATOR’S MANUAL

Version 2.0 02/2013 Part code: ELITE/M1

CONTENTS

PAGE INTRODUCTION BEFORE USE

Unpacking 4

SAFETY INFORMATION

English 5 Français 7 Deutsch 9 Italiano 11 Español 13

CONTACT INFORMATION Prime Elite SPECIFICATION INSTALLATION GENERAL SOFTWARE FEATURES

Introduction 20 Template programs 20 Incremented/decremented time and temperature 21 Gradient 21 Oligonucleotide Tm calculator 22

PROGRAMMING TEXT, TEMPERATURE AND TIME

Text entry 23 Temperature entry 23 Time entry 27

INSTRUMENT SETTINGS AND DEFAULTS

User preferences 30 Instrument settings 31 Program defaults 34 Service settings 35 Gradient calculator 35 Oligonucleotide Tm calculator 36

CREATE A NEW PROGRAM

Name the program 38 Insert/delete a stage 39 Insert/delete a step 40 Insert/delete a pause 40 Program options 40 Settings 44 View 44 Delete 45 Up and down arrows 45 Save 45 Cancel 45

RUN A PROGRAM

Running a program from the shortcut list 46 The pre-run screen 47

3 4

15 16 18 20

The run screen 48 Searching for a program not in the shortcut list 49 Running a program not on the shortcut list 51

EDITING A PROGRAM

Editing saved programs 53 Editing templates 53 Editing password-protected programs 54

CURRENT STATUS VIEW FILES

File searching 56 Viewing a program file 58 Viewing a temperature log file 58 Copying files 59 Deleting files 61

UPDATING THE Prime Elite SOFTWARE FAQs TECHNICAL SUPPORT AND SERVICING ADDITIONAL INFORMATION

User Maintenance 65 Fault Finding 65 Fuses 65 Insulation Testing 65

REPLACEMENT PARTS DECLARATION OF CONFORMITY

55 56

62 63 64 65

66 67

INTRODUCTION

The Prime Elite is an advanced gradient thermal cycler designed to accurately and rapidly control the temperatures of samples between 4ºC (or 20°C below ambient) and 100°C. It has many scientific applications including PCR, reverse transcription, ligation and sequencing. It is available in a range of block formats to allow for most commonly used consumables including 0.2ml x 96 well plates and

0.2ml tubes, 0.5ml tubes and 384 well plates. The heated lid automatically applies the correct pressure to suit the consumable used.

The Prime Elite is programmed via a 5.7” colour VGA LCD touch screen using simple and intuitive software. Programs and run progress can be viewed as a temperature/step graphical display, allowing the user to readily check the status or position within a run. Files can be saved with user names and/or keywords which allows for rapid searching and retrieval. A USB port on the front of the unit allows transfer of files to and from a USB memory stick and for the easy update of instrument software.

The thermal block incorporates the very latest in Peltier design featuring the Thermal Energy Recovery System (TERS®). This allows the Prime Elite to achieve ramp rates of up to 5ºC/s and is also energy saving, resulting in reduced running costs.

The Prime Elite can be used as a stand-alone unit or, for high-throughput applications, to control up to three additional Satellite units via USB connections. Satellite units can also be remotely controlled from a PC using the Techne Workbench software. Programs and temperature files are saved as text files for integration into a LIMS System.

BEFORE USE

Before using the Prime Elite please make sure you have read this manual carefully. If there is any doubt relating to the proper use of this equipment, the staff at Bibby Scientific Ltd. or your supplier will be happy to assist you.

UNPACKING

When unpacking the unit please ensure that the following have been removed from the packaging:

• Prime Elite with block

• Mains cables (UK, EU and US)

• USB cable

• Operator’s manual

• Warranty card

The user is advised to keep the original packaging in case the instrument ever needs to be returned for service or repair. Bibby Scientific Ltd. accepts no responsibility for damage incurred unless the unit is correctly packed and transported in its original packaging.

SAFETY INFORMATION

Please read all the information in this manual before using the Prime Elite.

WARNING

HIGH TEMPERATURES ARE DANGEROUS: they can cause serious burns to operators and ignite combustible material. Users should be aware of the following potential hazards:

• USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS

• DO NOT use combustible substances near hot objects

• DO NOT operate the instrument in the vicinity of inflammable liquids or gases

• DO NOT place any liquid directly into the instrument.

OPERATOR SAFETY

All operators of Techne equipment must have available the relevant literature needed to ensure their safety. It is important that only suitably trained personnel operate this equipment, in accordance with the instructions contained in this manual and with general safety standards and procedures. If the equipment is used in a manner not specified by Bibby Scientific Ltd. the protection provided by the equipment to the operator may be impaired.

All Techne instruments are designed to conform to international safety requirements and are fitted with an over-temperature cut-out. If a safety problem should be encountered, switch off the unit at the mains socket and remove the plug from the electricity supply.

INSTALLATION

The instrument should be carried using both hands. Never move or carry the instrument when in use or connected to the mains electricity supply.

1. All Techne instruments are supplied with a power cable; this may be integral or plug-in.

2. Before connecting the instrument to the mains electricity supply, check the voltage

against the rating plate (located on the back of the unit). Please note that the unit must be earthed to ensure proper electrical safety. Connect the mains cable to a suitable plug according to the table below.

Connections 220/240V, 50/60Hz 110V/120V, 50/60Hz

Live Brown Black Neutral Blue White Earth Green/yellow Green

3. UK ONLY: The fused plug supplied with the mains cable is fitted with a 10 amp fuse to protect the

instrument and the operator.

4. The units are rated to operate at 100-230V, 50/60Hz.

5. Place the unit on a suitable flat bench or in a fume cupboard if required, ensuring that the air inlet

vents on the underside are free from obstruction.

6. Plug the mains cable into the socket on the back of the instrument.

7. Switch on the instrument using the switch located on the front of the unit.

Replacement cable

Should the mains lead need replacement, a cable of 1mm2 of harmonized code H05VV-F connected to an IEC320 plug should be used. IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN.

ENVIRONMENTAL CONDITIONS

The Prime Elite is designed operate under the following conditions:

• Indoor use

• Ambient temperature range +5ºC to +40ºC

• Altitude to 2000m

• Relative humidity not exceeding 80%

• Mains supply fluctuations not exceeding 10%

• Over voltage category II IEC 60364-4-443

• Pollution degree 2

Note: The control specifications are quoted at an ambient temperature of 20ºC. The specification may deteriorate outside an ambient temperature of between 10ºC and 30ºC.

The instrument has been tested for radio frequency interference and is certified under EN61326.

WARRANTY

Bibby Scientific Ltd warrants this instrument to be free from defects in material and workmanship, when used under normal laboratory conditions for four (4) years. In the event of a justified claim, Bibby Scientific Ltd will replace any defective component or replace the unit free of charge. This warranty does not apply if damage is caused by fire, accident, misuse, neglect, incorrect adjustment or repair, damage by incorrect installation, adaption, modification, fitting of non-approved parts or repair by unauthorised personnel. Bibby Scientific Ltd liability is limited to the cost of repair or replacement of the product and excludes in particular, indirect and consequential loss, damage, costs or expenses, including but not limited to wasted time, materials and expenditure or loss of use, profit, production, revenue, expected savings or goodwill. To make a claim please contact the supplier of the instrument. This warranty is in addition to, and does not affect any statutory rights.

This manual has been prepared for the convenience of Techne’s customers and nothing in this manual shall be taken as a warranty, condition or representation concerning the description, merchantability, fitness for purpose or otherwise of the unit or components.

Notwithstanding the description and specification(s) of the instruments contained in the operator’s manual, Techne reserves the right to make such changes as it sees fit to the instruments or to any of the components.

L'INFORMATION DE SÛRETÉ

Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le Prime Elite.

AVERTISSEMENT

Les TEMPÉRATURES ÉLEVÉES SONT DANGEREUSES car elles peuvent provoquer de graves brûlures chez l’opérateur et enflammer les matériaux combustibles. Les utilisateurs doivent porter une attention toute particulière aux points suivants :

• PROCEDER AVEC PRUDENCE ET PORTER DES GANTS POUR SE PROTEGER LES MAINS

• NE PAS utiliser de matériaux combustibles auprès d’objets chauds.

• NE PAS utiliser l’appareil à proximité de liquides ou de gaz inflammables

• NE PAS verser de liquides directement dans l’appareil.

SECURITE DE L’OPERATEUR

Tous les utilisateurs de produits Techne doivent avoir pris connaissance des consignes et instructions nécessaires à la garantie de leur sécurité. Important: cet appareil doit impérativement être manipulé par un personnel qualifié et être utilisé selon les instructions données dans ce document, en accord avec les normes et procédures de sécurité générales. Dans le cas où cet appareil ne serait pas utilisé selon les consignes précisées par Bibby Scientific Ltd., la protection pour l’utilisateur ne serait alors plus garantie.

Tous les appareils Techne sont conçus pour répondre aux normes de sécurité internationales et sont dotés d’un coupe-circuit en cas de surchauffe. En cas de problème de sécurité, couper l’alimentation électrique au niveau de la prise murale et enlevez la prise connectée à l’appareil.

INSTALLATION

Porter l'appareil à deux mains. Ne jamais déplacer ou transporter l’appareil lorsqu’il est en fonctionnement ou branché à l’alimentation électrique.

1. Tous les appareils Techne sont livrés avec un câble d'alimentation, qui peut être intégré à l'appareil ou à raccorder.

2. Avant de raccorder l’appareil à l’alimentation électrique sur secteur, vérifier la tension

requise indiquée sur la plaque d’identification (située au dos de l’appareil). Il est

important que l’appareil soit relié à la terre pour assurer la protection électrique requise. Brancher le câble secteur sur une prise appropriée, voir tableau ci-après.

Connexions 220/240 V, 50/60 Hz 110/120 V, 50/60 Hz

Phase Marron Noir Neutre Bleu Blanc Terre Vert/jaune Vert

3. ROYAUME-UNI SEULEMENT: La prise avec fusible intégré fournie avec le câble secteur est munie d’un fusible 10 A destiné à protéger l’appareil et l’utilisateur.

4. Les unités sont évaluées pour fonctionner à 100-230V, 50/60Hz.

5. Placer l’appareil sur une surface plane ou si nécessaire sous une hotte d’aspiration, veiller à ce que les trous d’aération situés sous l’appareil ne soient pas obstrués.

6. Raccorder le câble d’alimentation à la prise située à l’arrière de l’appareil.

7. Allumer l'appareil utilisant le commutateur situé sur l'avant de l'unité.

Câble de rechange

S’il s’avère nécessaire de remplacer le cordon d’alimentation, utiliser un câble de 1 mm² conforme à la norme H05VV-F relié à une prise IEC320. EN CAS DE DOUTE, CONSULTER UN ELECTRICIEN

QUALIFIE.

CONDITIONS ENVIRONNEMENTALES

Le Prime Elite est conçu pour fonctionner dans les conditions suivantes:

• Pour un usage intérieur seulement

• Température ambiante +5°C à +40°C

• Altitude inférieure à 2000m

• Humidité relative ne dépassant pas 80%

• Fluctuations de l’alimentation n’excédant pas 10% de la valeur nominale

• Catégorie II IEC 60364-4-443 de surtension

• Degré de pollution 2

Remarque: Les paramètres sont indiqués pour une température ambiante de 20°C. Ces caractéristiques peuvent se détériorer en dehors d’une température ambiante de 10 à 30°C.

L’appareil a été testé en matière de radiofréquences et est certifié selon la norme EN61326.

GARANTIE

Bibby Scientific Ltd garantit cet instrument contre tout vice de matière et de fabrication, quand il est utilisé dans des conditions normales de laboratoire pendant quatre (4) ans. En cas de réclamation justifiée, Bibby Scientific Ltd procèdera gratuitement au remplacement de tout élément défectueux ou au remplacement de l'appareil. Cette garantie ne s'applique pas aux dommages provoqués par les incendies, accidents, mésusages, négligences, réglages ou réparations incorrects, aux dommages dus à une installation incorrecte, adaption, modification, au montage de pièces non homologuées ou réparation par un personnel non autorisé. La responsabilité de Bibby Scientific Ltd se limite aux coûts de réparation ou de remplacement du produit et exclut, en particulier, les pertes, dommages, coûts ou dépenses indirects et conécutifs, parmi lesquels, mais sans s'y limiter, les pertes de temps, de matériaux et les dépenses ou perte d'utilisation, de profit, de production, de chiffre d'affaires, d'économies escomptées ou écarts d'acquisition. En cas de réclamation, veuillez prendre contact avec le fournisseur de cet appareil. Cette garantie s'ajoute à vos droits légaux et n'affecte en rien ces derniers.

Le présent manuel a été exclusivement rédigé à l’attention des clients de la marque Techne et rien dans son contenu ne doit être pris comme une garantie, une condition ou une affirmation concernant la description, la commercialisation, l’adéquation à un usage particulier de l'appareil ou de ses composants.

Malgré la description et les caractéristiques techniques des appareils données dans le manuel de l’utilisateur, la société Techne se réserve le droit d’apporter les changements nécessaires à l’appareil ou à tout élément qui entre dans sa composition.

SICHERHEITS- INFORMATIONEN

Lesen Sie diese Anleitung vor Verwendung des Prime Elite bitte sorgfältig durch.

ACHTUNG

HOHE TEMPERATUREN STELLEN EINE GEFAHRENQUELLE DAR. Sie können schwere Brandverletzung verursachen und brennbare Stoffe entzünden. Der Benutzer sollt sich mit den möglichen Gefahren vertraut machen:

• UMSICHTIG VORGEHEN UND SCHUTZHANDSCHUHE TRAGEN

• KEINE brennbaren Stoffe in der Nähe heißer Gegenstände verwenden

• Das Gerät NICHT in der Nähe entzündlicher Flüssigkeiten oder Gase betreiben

• Flüssigkeiten NICHT direkt auf das Gerät auftragen.

SICHERHEIT DES BEDIENPERSONALS

Alle Benutzer von Techne Geräten müssen Zugang zu der entsprechenden Literatur haben, um ihre Sicherheit zu gewähren. Es ist wichtig, daß diese Geräte nur von entsprechend geschultem Personal betrieben werden, das die in dieser Gebrauchsanweisung enthaltenen Maßnahmen und allgemeine Sicherheitsbestimmungen und - vorkehrungen beachtet. Wenn das Gerät anders eingesetzt wird als vom Hersteller empfohlen, kann dies die persönliche Sicherheit des Anwenders beeinträchtigen.

Die Geräte von Techne entsprechen den internationalen Sicherheitsbestimmungen und sind mit einem automatischen Übertemperaturabschalter ausgestattet. Wenn ein Sicherheitsproblem auftreten sollte, muß das Gerät ausgeschaltet und vom Stromnetz getrennt werden.

INBETRIEBNAHME

Das Gerät mit beiden Händen tragen. Das Gerät unter keinen Umständen transportieren, wenn es in Betrieb ist, oder während das Gerät noch am Netz angeschlossen ist.

1. Alle Geräte von Techne werden mit einem Netzkabel geliefert, das entweder eingesteckt wird

oder fest mit dem Gerät verbunden ist.

2. Vor dem Anschluss bitte kontrollieren, ob die Stromversorgung den Angaben auf dem

Typenschild (auf der Geräterückseite) entspricht. Um die elektrische Sicherheit zu gewährleisten, muss dieses Gerät geerdet werden. Schließen Sie das Netzkabel entsprechend der folgenden Tabelle an einen geeigneten Stecker an.

Anschluss 220/240V, 50/60Hz 110V/120V, 50/60Hz

Phase Braun Schwarz Neutral Blau Weiß Erde Grün/Gelb Grün

3. NUR FÜR GROSSBRITANNIEN: der mit dem Netzkabel gelieferte Sicherungsstecker enthält

eine 10 Amp. Sicherung zum Schutz des Geräts und des Anwenders.

4. Die Maßeinheiten werden veranschlagen, um an 100-230V, 50/60Hz zu funktionieren.

5. Stellen Sie das Gerät auf einen geeigneten ebenen Tisch oder in einem Abzugsschrank auf und

sorgen Sie dafür, dass die Lufteinlassschlitze auf der Geräteunterseite nicht blockiert sind.

6. Stecken Sie das Netzkabel in die Buchse auf der Geräterückseite ein.

7. Schalten Sie das Instrument unter Verwendung des Schalters an, der auf der Frontseite der

Maßeinheit gelegen ist.

Ersatzkabel

Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1 mm2 Adernquerschnitt und Europastecker (IEC 320) benötigt. IM ZWEIFELSFALL EINEN ELEKTRO-

FACHMANN HINZUZIEHEN.

UMWELTBEDINGUNGEN

Der Prime Elite ist für den Einsatz unter folgenden Bedingungen ausgelegt:

• Gebrauch in Innenräumen

• Umgebungstemperatur zwischen +5ºC to +40ºC

• Höhe: bis zu 2000 m

• Relative Feuchte nicht über 80%

• Netzspannungsschwankungen nicht über 10%

• Überspannungsklasse 2 IEC 60364-4-443

• Verschmutzungsgrad 2

Hinweis: Die Gerätespezifikationen beziehen sich auf eine Umgebungstemperatur von 20ºC und können sich außerhalb des Bereichs 10ºC bis 30ºC verschlechtern.

Das Gerät wurde auf HF-Störeinflüsse geprüft und entspricht den EMV-Bedingungen nach EN61326.

GARANTIE

Bibby Scientific Ltd gewährleistet, dass dieses Gerät vier (4) Jahre lang keine Material- oder Herstellungsfehler aufweist, wenn es unter normalen Laborbedingungen verwendet wird. In einem berechtigten Garantiefall wird Bibby Scientific Ltd alle defekten Komponenten oder das Gerät kostenlos austauschen. Diese Garantie gilt nicht, wenn der Schaden durch ein Feuer, einen Unfall, Missbrauch, Nachlässigkeit, falsche Einstellungen oder Reparaturen, falsche Installation, Adaptierung, Veränderung, den Einbau nicht zugelassener Teile oder die Reparatur durch nicht autorisiertes Personal verursacht wurde. Die Garantie von Bibby Scientific Ltd ist beschränkt auf die Kosten der Reparatur oder den Austausch des Produkts und schließt ausdrücklich indirekte und nachfolgende Verluste, Schäden, Kosten oder Ausgaben aus, einschließlich aber nicht beschränkt auf Zeit-, Material- und Geldverlust oder den Verlust des Gebrauchs, von Gewinn, Produktion, Einkommen, erwarteten Einsparungen und des Firmenwerts. Um einen Garantiefall zu melden, wenden Sie sich bitte an den Vertriebspartner des Geräts. Diese Garantie gilt zusätzlich zu und nicht anstelle von geltendem Recht.

Diese Anleitung wurde zur Information der Kunden von Techne erstellt und stellt in keinster Weise eine Gewährleistung, Bedingung oder Darstellung bezüglich der Beschreibung, Marktgängigkeit oder Zweckdienlichkeit dieser Geräte oder Bauteile dar.

Unabhängig von Beschreibung und Spezifikation(en) des hier beschriebenen Geräts behält sich Techne das Recht vor, Änderungen an diesem Gerät oder dessen Bauteilen vorzunehmen.

INFORMAZIONI DI SICUREZZA

Leggere attentamente il presente manuale prima di usare il Prime Elite.

AVVERTENZA

Le ALTE TEMPERATURE SONO PERICOLOSE in quanto possono provocare serie ustioni agli operatori e dare fuoco al materiale combustibile. Gli utenti devono conoscere i seguenti pericoli potenziali:

• PRESTARE ATTENZIONE ED INDOSSARE GUANTI PROTETTIVI PER LE MANI

• NON usare sostanze combustibili vicino ad oggetti caldi

• NON mettere in funzione lo strumento nei pressi di liquidi o gas infiammabili

• NON collocare alcun tipo di liquido direttamente nello strumento.

SICUREZZA DELL'OPERATORE

Il personale che utilizza l’apparecchiatura Techne deve avere a disposizione la documentazione necessaria al fine di assicurare la loro incolumità. È importante che solo personale adeguatamente addestrato utilizzi questo apparecchio, in conformità alle istruzioni contenute in questo manuale e nel rispetto delle normative e procedure generali di sicurezza. Se l’apparecchio è utilizzato in modo non specificato da Bibby Scientific Ltd., la protezione fornita dall’apparecchiatura all’utilizzatore potrebbe essere a rischio.

Tutte le unità Techne sono state progettate in conformità ai requisiti internazionali di sicurezza e sono equipaggiate con un interruttore anti surriscaldamento. Se si dovesse verificare qualche problema di sicurezza, disconnettere l’apparecchio dalla rete.

INSTALLAZIONE

Occorre trasportare lo strumento usando entrambe le mani. Non spostare né trasportare lo strumento quando è in funzione o collegato all’alimentazione elettrica di rete.

1. Tutti gli strumenti Techne sono forniti con un cavo di alimentazione; può essere integrale o plugin.

2. Prima di collegare lo strumento all'alimentazione elettrica di rete, controllare la

tensione confrontandola con la targhetta riportante i valori nominali (si trova sul retro dell'unità). Notare che al fine di garantire la corretta sicurezza elettrica, occorre che l'unità sia messa a terra. Collegare il cavo di rete ad una presa idonea secondo la tabella riportata alla pagina successiva.

Connessione 220/240V, 50/60Hz 110V/120V, 50/60Hz

Sotto tensione Marrone Nero Neutro Blu Bianco Terra Verde/giallo Verde

3. SOLO REGNO UNITO: la spina con fusibile fornita con il cavo di rete è dotata di un fusibile da 10 Amp per proteggere lo strumento e l'utente.

4. Le unità sono stimate funzionare a 100-230V, 50/60Hz.

5. Collocare l'unità su un banco piano idoneo o in una cappa aspirante se necessario, assicurandosi che gli sfiati delle prese d'aria nella parte inferiore non siano ostruiti.

6. Inserire il cavo di rete nella presa che si trova sul retro dello strumento.

7. Accendere lo strumento per mezzo dell'interruttore situato sulla parte anteriore dell'unità.

Cavo di ricambio

Qualora occorra sostituire il cavo di rete, si dovrà utilizzare un cavo di 1mm2 codice armonizzato H05VV-F collegato ad una spina IEC 320. IN CASO DI DUBBIO, RIVOLGERSI A UN

ELETTRICISTA QUALIFICATO.

CONDIZIONI AMBIENTALI

Il Prime Elite è stato progettato per funzionare nelle seguenti condizioni:

• uso interno

• range di temperatura ambiente da +5ºC a +40ºC

• altitudine massima 2000 m.

• umidità relativa non superiore all’80%

• oscillazione dell’alimentazione di rete non superiore al 10%

• categoria di sovratensione II IEC 60364-4-443

• grado di inquinamento 2

Nota: le specifiche di controllo sono indicate ad una temperatura ambiente di 20ºC. Le specifiche potrebbero peggiorare fuori da una temperatura ambiente compresa tra 10ºC e 30ºC.

Lo strumento è stato collaudato per interferenze da radiofrequenze ed è certificato secondo la norma EN61326.

GARANZIA

Bibby Scientific Ltd garantisce che, per un periodo di quattro (4) anni e se utilizzato nelle normali condizioni di laboratorio, questo strumento non presenterà alcun difetto dei materiali o di fabbricazione. In caso di reclamo giustificato, Bibby Scientific Ltd sostituirà qualsiasi componente difettoso o rimpiazzerà l'unità in modo gratuito. Questa garanzia non si applica ai danni causati da incendio, incidenti, utilizzo improprio, negligenza, regolazioni o riparazioni scorrette, danni dovuti a installazione non corretta, adattamenti, modifiche o installazione di parti non approvate oppure riparazioni eseguite da personale non autorizzato. La responsabilità di Bibby Scientific Ltd è limitata al costo della riparazione o della sostituzione del prodotto ed esclude in particolare qualsiasi perdita indiretta o secondaria, danno, costo o spesa, ivi inclusi, a titolo di esempio e in modo non esaustivo, perdite di tempo, materiali e spese oppure perdite di utilizzo, profitto, produzione, ricavo, risparmio atteso o avviamento. Per esporre un reclamo, contattare il fornitore dello strumento. Questa garanzia è in aggiunta ai diritti di legge e non ha alcun effetto su di essi.

Il presente manuale è stato preparato ad uso dei clienti di Techne e niente di quanto in esso contenuto costituisce garanzia, condizione o rappresentanza riguardo la descrizione, la commerciabilità, l'idoneità allo scopo o altrimenti dell'unità o dei componenti.

Nonostante la descrizione e le specifiche dello strumento contenuti nel manuale dell'operatore, Techne si riserva il diritto di apportare le modifiche ritenute opportune agli strumenti o a qualsiasi loro componente.

INFORMACIÓN DE SEGURIDAD

Lea atentamente este manual antes de utilizar el Prime Elite.

ADVERTENCIA

LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que pueden ocasionar quemaduras graves a los operarios y prender el material combustible. Los usuarios deben conocer los posibles riesgos:

• TENGA CUIDADO Y LLEVE GUANTES DE PROTECCIÓN PARA PROTEGERSE LAS MANOS

• NO utilice sustancias combustibles cerca de objetos calientes

• NO utilice el instrumento cerca de líquidos o gases inflamables

• NO coloque un líquido directamente en el instrumento.

SEGURIDAD DEL OPERARIO

Todos los usuarios de equipos Techne deben disponer de la información necesaria para asegurar su seguridad. De acuerdo con las instrucciones contenidas en este manual y con las normas y procedimientos generales de seguridad, es muy importante que sólo personal debidamente capacitado opere estos aparatos. De no ser así, la protección que el equipo le proporciona al usuario puede verse reducida.

Todos los equipos Techne han sido diseñados para cumplir con los requisitos internacionales de seguridad y traen incorporados un sistema de desconexión en caso de sobre temperatura. En caso de que surgiera un problema de seguridad, desconecte el equipo de la red.

INSTALACIÓN

El instrumento se debe transportar con las dos manos. No mueva ni lleve el instrumento cuando se utilice o esté conectado al suministro eléctrico principal.

1. Todos los instrumentos Techne se suministran con un cable de alimentación, que puede ser integrado o ‘enchufable’.

2. Antes de conectar el instrumento al suministro eléctrico, compruebe que el voltaje

coincida con el indicado en la placa de régimen (situada en la parte trasera de la unidad). El instrumento debe disponer de una toma de tierra para garantizar la seguridad eléctrica adecuada. Conecte el cable de alimentación a un enchufe adecuado según la siguiente tabla.

Conexión 220/240V, 50/60Hz 110V/120V, 50/60Hz

Con corriente Marrón Negro Neutro Azul Blanco Toma de tierra Verde/amarillo Verde

3. SÓLO PARA EL REINO UNIDO: El enchufe suministrado con el cable de alimentación incluye un fusible de 10 amperios para ofrecer protección al instrumento y al usuario.

4. Las unidades se clasifican para funcionar en 100-230V, 50/60Hz.

5. Sitúe la unidad sobre una mesa plana o en una campana de laboratorio si es necesario, y asegúrese de que los orificios de ventilación situados en la parte inferior no tienen ninguna obstrucción.

6. Conecte el cable de alimentación en el enchufe situado en la parte trasera del instrumento.

7. Encienda el instrumento usando el interruptor situado en el frente de la unidad.

Cable de repuesto

Si es necesario sustituir el cable de alimentación, se debe utilizar un cable de 1mm2 de código armonizado H05VV, conectado a un enchufe IEC320. EN CASO DE DUDA, PÓNGASE EN

CONTACTO CON UN ELECTRICISTA.

CONDICIONES AMBIENTALES

El Prime Elite está diseñado para utilizarse en las condiciones siguientes:

• Uso en interior

• Intervalo de temperatura ambiente +5ºC a +40ºC

• Altitud: hasta 2000 m

• Humedad relativa no superior al 80%

• Fluctuaciones del suministro eléctrico no superiores al 10%

• Categoría de sobrevoltaje II IEC 60364-4-443

• Nivel de contaminación 2

Nota: Las especificaciones de control corresponden a una temperatura ambiental de 20ºC. Las especificaciones pueden empeorar si se utiliza el instrumento fuera del intervalo de temperatura comprendido entre 10ºC y 30ºC.

Se han realizado pruebas para comprobar la interferencia de radiofrecuencia del instrumento, el cual cumple la normativa EN61326.

GARANTÍA

Bibby Scientific Ltd garantiza que este equipo estará libre de defectos de materiales y mano de obra, si se utiliza en condiciones normales de laboratorio, durante un período de cuatro (4) años. En caso de reclamación justificada, Bibby Scientific Ltd sustituirá cualquier componente defectuoso o cambiará la unidad gratuitamente. Esta garantía no es aplicable si los daños han sido ocasionados por incendios, accidentes, usos inapropiados, negligencias, ajustes o reparaciones incorrectas, así como si se trata de daños ocasionados por la instalación, adaptación, modificación o incorporación en el instrumento de piezas no aprobadas o su reparación por personal no autorizado. La responsabilidad de Bibby Scientific Ltd se limita al coste de la reparación o sustitución del producto, y excluye específicamente pérdidas, daños, costes o gastos indirectos o accidentales, incluyendo pero sin estar limitado a ello, pérdidas de tiempo, materiales y gastos o pérdida de uso, beneficios, producción, ingresos, ahorros previstos u oportunidades comerciales. Si desea realizar una reclamación, póngase en contacto con el suministrador del equipo. Esta garantía complementa y no afecta a sus derechos legales.

Este manual se ha preparado con una finalidad informativa para los clientes de Techne y ninguna parte del manual se deberá considerar como una garantía, condición o reflejo con respecto a la descripción, comerciabilidad, idoneidad para un fin determinado o de otro tipo de la unidad o sus componentes.

Con independencia de la descripción y las especificaciones del instrumento que se indican en el manual del operario, Techne se reserva el derecho de realizar cambios en el instrumento o en cualquiera de sus componentes cuando lo estime oportuno.

CONTACT INFORMATION

North and South America

France

Asia

Middle East

For technical, sales or servicing information, contact your local Techne distributor or:

Bibby Scientific Ltd. Beacon Road Stone Staffordshire ST15 0SA UK Tel: +44 (0)1785 812121 Fax: +44 (0)1785 810405 E-mail: sales@bibby-scientific.com

www.techne.com

Bibby Scientific Limited Bâtiment Le Deltaparc Parc Silic PN2 7 rue du Canal BP 55437 VILLEPINTE 95944 ROISSY Charles de Gaulle France Tel: +33(0)148 63 78 03 Fax: +33(0)148 63 78 01 E-mail: ventes@bibby-scientific.com

www.bibby-scientific.com

Bibby Scientific Middle East Ltd. PO Box 27887, Engomi 2433 Nicosia Cyprus Tel: +357 22 660 423 Fax: +357 22 660 424 e-mail: sales@bibby-scientificme.com

Bibby Scientific US Inc. t/a Techne Inc. 3 Terri Lane, Suite 10 Burlington, N.J. 08016 USA Toll Free (in NA): 800-225-9243 Tel: +1 609 589 2560 Fax: +1 609 589 2571 E-mail: labproducts@techneusa.com

www.techneusa.com

Bibby Scientific - Singapore Prudential Tower, Level 26 30 Cecil Street Singapore 049712 Tel: +65 6631 2976 Fax: +44 (0) 1785 810405 e-mail: info@bibby-scientific.com

www.bibby-scientific.com

PRIME ELITE SPECIFICATION

TEMPERATURE

Block temperature range 4°C or 20ºC below ambient to 100ºC Block uniformity <±0.3ºC Temperature accuracy <±0.2ºC Gradient availability 10°C to 100°C Maximum gradient 30ºC (16ºC with the 384-well block) Minimum gradient 1ºC Column uniformity with a 10ºC gradient <±0.3ºC Pre-run sample cooling Yes, 4ºC Temperature set point adjustment 0.1ºC

HEATING/COOLING RATE

Maximum heating rate 5.0ºC/s Minimum heating rate 0.1ºC/s Maximum cooling rate 3.0ºC/s Minimum cooling rate 0.1ºC/s

HEATED LID

Selectable heated lid temperature 35 to 115ºC or off Pre-heat lid Yes Warm up time from ambient <2 min Over-temperature cut-out Yes Regulated heated lid pressure Automatic

The heated lid is only operational if the block temperature is set above 35ºC

PROGRAMMING*

Program interface 5.7” colour VGA LCD touch screen Maximum number of programs stored 1000 Maximum number of stages per program 99 Maximum number of steps per stage 99 Maximum number of cycles per stage 99 Programmable ramp rate Yes, 0.1ºC/s steps Maximum hold time 99h 59m 59s Minimum hold time 1s Gradient calculator Yes Incremented/decremented temperature Yes Incremented/decremented time Yes Pause facility Yes Program naming Alpha numeric plus symbols Password protection Yes Keyword selection Yes Run completion time Yes End of program alarm Yes Auto resume on power failure Yes with options of 10min, 30min, 1h or always Oligonucleotide Tm calculator Yes, based on the Nearest-Neighbour method1

*Programming for Satellite units is via a Prime Elite unit or Techne Workbench PC software.

Breslauer, K.J.; Frank, R.; Blocker, H. and Marky, L.A. (1986) Proc. Natl. Acad. Sci. USA 83, pp

3746-3750.

INTERCONNECTIVITY

Connection to PC control software Yes (Satellite units only) Software updates Free of charge from www.techne.com

COMMUNICATION PORTS

Prime Elite: Connections for Satellite units: 3 x USB ports on rear of unit Connection for memory stick: USB port on front of unit

Satellite unit: Connection to PC or Prime Elite: USB port on rear of unit

DIMENSIONS

Height (mm) 312 Width (mm) 276 Depth (mm) 375 Weight (kg) 14

POWER

Voltage 100-230V Power 1000W

INSTALLATION

See also the Safety Information sections on pages 5 to 14.

Front view

Prime Elite Prime Elite Satellite

1. Prime Elite 5.7” colour VGA LCD touch screen display.

2. Drawer release push panel.

3. Prime Elite USB port for memory stick.

4. Power on/off

5. Prime Elite Satellite ID and status indicator.

Rear view

1. Lifting Handle.

2. USB ports for connection of Satellite units (Prime Elite only).

3. USB port for connection to PC or Prime Elite (Satellite units only).

4. Mains cable inlet.

Installation

1. Place the unit on a suitable flat bench, ensuring that the air inlet vents on the front and rear are

free from obstruction. Two units may be stacked one on top of the other (Prime Elite on a Satellite or Satellite on Satellite).

2. Plug the mains cable into the socket on the back of the instrument.

3. Connect to the mains electricity supply with the plug provided or one wired correctly for the

supply. Switch the power ON using the switch located on the front of the unit. The front display will then light up.

4. Open the drawer by pushing on the front drawer panel then pull the drawer forwards to access the

block.

5. Place the samples in the block. If individual tubes or strip tubes are being used, space these out

evenly across the block to equalise the pressure from the heated lid.

6. To close the drawer, push back into the unit until it locks in place.

Connecting Satellite units to the Prime Elite

The Prime Elite has three USB ports on the rear of the unit for connection of Satellite units. Three Satellite units can be connected directly to the Prime Elite as shown in the configuration below.

USB memory stick

GENERAL SOFTWARE FEATURES

Parameter

2 Step

Template

3 Step

Template

RT-

PCR

Template

2 Step

Template

3 Step

Template

RT-

PCR

Template

Stage

INTRODUCTION

The Prime Elite software allows the user to quickly and easily create thermal cycling programs using a simple and clear touch screen format. The software is structured into five modules which are accessed from the Home Screen. These allow the user to:

• Run an existing program, selecting from templates or programs saved on the unit.

• Create a new program.

• Set instrument and program defaults and

preferences. Includes calculator tools.

• View the current status of connected units.

• View and search for files stored on the unit.

TEMPLATE PROGRAMS

To help with programming, a number of program templates are installed on the unit which are available to copy and edit or can be run directly without changes. Details of the installed program templates are given below.

Instrument and program defaults

Heated Lid 105ºC 105ºC 105ºC Heated lid before program On On On Sample cooling On On On Pause before program Off Off Off Initial denaturation 94ºC, 05m00s 94ºC, 05m00s 94ºC, 05m00s Hot start Off Off Off Final extension 72ºC, 05m00s 72ºC, 05m00s 72ºC, 07m00s Final hold 10ºC 10ºC 10ºC

Thermal cycling conditions

Number of cycles 30 30 1 Step 1 94.0ºC, 00m30s 94.0ºC, 00m30s 45.0ºC, 40m00s Step 2 60.0ºC, 01m00s 55.0ºC, 00m30s 94.0ºC, 05m00s Step 3 72.0ºC, 00m30s

Number of cycles 40 Step 1 94.0ºC, 01m00s Step 2 55.0ºC, 00m50s Step 3 72.0ºC, 01m00s

The Ice Bucket and Ligation programs are simple temperature holds, with the former having an infinite

Lowest temperature

Set temperature

Highest temperature

Gradient

hold at 4ºC and the latter at 15ºC. In both of these programs the heated lid is switched off and the initial denaturation, hot start and final extension functions disabled.

INCREMENTED/DECREMENTED TIME AND TEMPERATURE

The software allows the user to either increment or decrement the time and/or temperature of a step within a cycling stage. These features are used with applications such as touchdown PCR where the annealing temperature is gradually decreased during the cycling process and long PCR, where due to the size of the product, long annealing/extension times are required and the extension time is increased by 15 to 20s per cycle during the final stages of the reaction.

GRADIENT

The gradient feature of the Prime Elite can be useful in optimising the annealing conditions for primers. A gradient can be set around a temperature in any step of a program. The set temperature is the temperature in the middle columns of the block and the gradient is the variation at the two extremes of the block; the left hand columns being the coolest and the right hand columns the hottest.

The maximum temperature gradient range which can be set is 30ºC and the minimum is 1ºC; the lowest temperature is 10ºC or 20ºC below ambient and the hottest temperature is 100ºC. Examples are given in the table below:

1 2 3 4 5 6 7 8 9 10 11 12

A B

B C

C D

D E

E F

F G

G H

Lowest

temperature

Set

temperature

Gradient

Highest

temperature

A gradient calculator is included in the Settings module so the user can obtain an accurate estimation of the temperature of each column during the gradient stage. The temperatures for any particular gradient are calculated from an algorithm stored in the block firmware.

(ºC)

50 55 60 10 10 25 40 30 80 90 100 20

(ºC)

OLIGONUCLEOTIDE TM CALCULATOR

One of the most important parameters in a PCR is the annealing temperature of the reaction. This can be determined theoretically using various software programmes to calculate the melting temperature (Tm) of the primers.

The Tm is defined as the temperature at which half the DNA strands are single stranded and half are double stranded when base paired to a complimentary strand. Using annealing temperatures that are well below the Tm of the primers can result in mismatching, false priming and may lead to primer-dimer artefacts. Annealing temperatures that are significantly higher than the primer Tm may result in reduced priming or prevent priming altogether.

The Prime Elite uses the Nearest-Neighbour method for calculating the Tm of an oligonucleotide1.

The Tm is calculated using the following equation:

Tm =

{(∆Hº x 1000)/(A + ∆Sº + R ln(C/4))} - 273.15 + 16.6 x log[Na+]

Where:

∆H

= The sum of the Nearest-Neighbour enthalpy changes (Kcal/mol)

A = Helix initiation correction constant

∆S

= The sum of the Nearest-Neighbour entropy changes (cal K

R = The Gas Constant (1.99 cal K

mol

)

mol

)

C = Concentration of the oligo (M) [Na+] = Concentration of monovalent ions (M)

Breslauer, K.J.; Frank, R.; Blocker, H. and Marky, L.A. (1986) Proc. Natl. Acad. Sci. USA 83, pp

3746-3750.

The Oligonucleotide Tm calculator can be found in the Settings module where the user can input the oligonucleotide sequence, its concentration in the reaction and the salt concentration of the reaction mix.

PROGRAMMING TEXT, TEMPERATURE AND TIME

This section gives instructions for the basic programming of text, temperature and time which are required in later sections.

TEXT ENTRY

When giving a name to a program or stage, or when entering a keyword or a password, a text entry screen will be opened.

Touch individual characters to create the name.

•

For words with all upper case letters touch

CAPS

•

Touch

upper case.

•

For numbers touch

•

For commonly used symbols touch

•

Touch the

between characters.

•

To clear the typed name, touch

•

To delete the last character touch

Once the name is complete, touch OK.

TEMPERTURE ENTRY

When touching a temperature parameter a temperature entry screen will open. There are two forms of temperature entry screen:

•

simple

and program parameters such as initial denaturation and final extension.

•

advanced

options for introducing an incremented/decremented step temperature, for example in touchdown PCR, or a temperature gradient.

SHIFT

if only the first letter is required in

123

SYM

space bar

form for adjusting the temperature of the instrument parameters such as the heated lid

form when programming step temperatures within a cycling stage that includes the

(not labelled) for spaces

CLR

DEL

Simple temperature entry

•

Enter the temperature by typing in the required

value. The decimal point can be used for 0.1ºC resolution.

•

To clear the entry press

•

If an entered temperature is invalid, a prompt will

indicate the acceptable range.

•

Once the temperature is correct, touch OK to

return to the previous screen.

•

Touch

no changes are required.

Cancel

to return to the previous screen if

DEL

Advanced temperature entry

increase the

step

temperature

per cycle

•

First ensure the number of cycles for the stage has been set then enter the required hold

temperature as described above.

Note

: Increase/Decrease Per Cycle will be inactive unless the number of cycles set is greater than 1.

•

For an

the button below function on. Next touch the temperature entry button below this.

A new screen will open which is similar to the simple temperature entry screen. The maximum increase in temperature per cycle (based on the number of cycles and the temperature limits of the unit) will be shown.

•

Enter the required value.

•

Touch

•

If an entered incremented temperature is invalid,

a prompt will indicate the acceptable range.

•

Touch OK to accept and return to the previous

screen.

The increase in temperature per cycle will now be displayed.

•

Touch OK again to return to the programming

screen.

•

Touch

screen if no changes are required.

increase

DEL

Cancel

in temperature per cycle, touch

Increase Per Cycle

to clear the entry.

to return to the programming

to turn this

The temperature value will now appear orange in

decrease the

step

temperature

per cycle

the programming screen indicating that it contains a modified function.

•

For a

A new screen will open which is similar to the simple temperature entry screen. The maximum decrease in temperature per cycle (based on the number of cycles and the temperature limits of the unit) will be shown.

•

Enter the required value.

•

Touch

•

If an entered decremented temperature is

•

Touch OK to return to the previous screen.

The decrease in temperature per cycle will now be displayed.

•

Touch OK again to return to the programming

•

Touch

decrease

the button below this function on. Next touch the temperature entry button below this.

DEL

invalid, a prompt will indicate the acceptable range.

screen.

Cancel

screen if no changes are required.

in temperature per cycle, touch

Decrease Per Cycle

to clear the entry.

to return to the programming

to turn

The temperature value will now appear orange in

To enter a temperature gradient

on a step

the programming screen indicating that it contains a modified function.

Note:

It is not possible to both increase and

decrease the temperature of the same step.

•

Touch the button below

to turn this function on. Next touch the temperature entry button below this.

A new screen will open which is similar to the simple temperature entry screen. The maximum temperature gradient (based on the set hold temperature and the temperature limits of the unit) will be shown.

•

Enter the required value.

•

Touch

•

If an entered gradient temperature is invalid, a

prompt will indicate the acceptable range.

•

Touch OK to return to the previous screen. The

temperature gradient for the step will now be displayed.

•

Touch OK again to return to the programming

screen.

•

Touch

screen if no changes are required.

DEL

to clear the entry.

Cancel

to return to the programming

Temperature Gradient

The temperature value will now appear orange in

•

the programming screen indicating that it contains a modified function.

TIME ENTRY

When touching a time parameter a time entry screen will open. There are two forms of time entry screen:

•

simple

extension.

•

advanced

options for introducing incremented/decremented time, for example in long PCR.

form for adjusting the time of the program parameters such as initial denaturation and final

form when programming step hold times within a cycling stage that includes the

Simple time entry

Enter the time using the + or – buttons to

increase or decrease the number of hours, minutes or seconds.

•

Using the – button with the value set at 0 will

scroll to the maximum permitted value.

•

Touching

selector and just display selectors for minutes and seconds.

•

Once the time is correct, touch OK to return to

the previous screen.

•

Touch

no changes are required.

Hide Hours

Cancel

will remove the hour time

to return to the previous screen if

Advanced time entry

•

First ensure the number of cycles for the stage has been set then enter the required hold time as

described above.

Note

: Increase/Decrease Per Cycle will be inactive unless the number of cycles set is greater than 1.

increase the

step

hold time per cycle

•

For an

button below function on. Next touch the time entry button below this.

A new screen will open which is similar to the simple time entry screen. The maximum increase in hold time per cycle (based on the number of cycles and the time limits of the unit) will be shown.

•

Enter the required value using the + and -

buttons.

•

If an entered incremented time is invalid, a

prompt will indicate the acceptable range.

•

Touch OK to return to the previous screen.

The increase in hold time per cycle will now be displayed.

•

Touch OK again to return to the programming

screen.

•

Touch

screen if no changes are required.

The time value will now appear orange in the programming screen indicating that it contains a modified function.

increase

Increase Per Cycle

Cancel

in hold time per cycle, touch the

to turn this

to return to the programming

decrease the

step hold time per cycle

•

For a

A new screen will open which is similar to the simple time entry screen. The maximum decrease in hold time per cycle (based on the number of cycles and the time limits of the unit) will be shown.

•

Enter the required value.

•

If an entered decremented time is invalid, a

•

Touch OK to return to the previous screen.

The decrease in hold time per cycle will now be displayed.

•

Touch OK again to return to the programming

•

Touch

The time value will now appear orange in the programming screen indicating that it contains a modified function.

Note:

decrease the time of the same step.

decrease

button below function on. Next touch the time entry button below this.

prompt will indicate the acceptable range.

screen.

Cancel

screen if no changes are required.

It is not possible to both increase and

in hold time per cycle, touch the

Decrease Per Cycle

to return to the programming

to turn this

INSTRUMENT SETTINGS AND DEFAULTS

Home Screen

Settings

From the

•

Touch any of the buttons to view and edit the

settings.

•

Touch

•

Once all the settings and defaults have been set

as required, touch and return to the Home Screen.

•

If you do not wish to change any settings, touch

Cancel

More Settings

to return to the Home Screen.

USER PREFERENCES

Language

•

Touch the

language. The options are English, French, Spanish, Italian and German.

Temperature Display

•

Touch the

required temperature units. The options are ºC and ºF.

Home Screen File List Sort

This sets the user preference for listing the program shortcuts on the Home Screen.

•

Touch the button to set the required sorting

preference. The options are recently used first or most used first.

•

Touch

preferences.

Select Multiple Items in File Manager

This allows either multiple files or only single files to be selected for copying and deleting when using the File Manager in the View files module.

•

Touch to select on or off.

•

Touch

Language

Temperature units

More Settings

Back

to return to the Settings menu.

, touch the

to access further options.

Save

to save the changes

button to access the instrument Settings menu.

button to set the required

button to set the

for further user

INSTRUMENT SETTINGS

Temperature Log Recording

To record the temperature logs (block temperature profile) of each run, touch the button to set this to Always. If temperature logs are not required, set to Never.

Auto Resume On Power Loss

This determines the time limit after which the unit will restart after a power failure. For example, if the Auto Resume is set to 30 minutes, the unit will restart at the beginning of the step at which it was interrupted if the power failure lasted for 30 minutes or less but not if it was for longer.

Setting Auto Resume to Always will always allow the unit to re-start irrespective of the length of the power failure.

Date and Time Settings

Use these settings to set the current date, time and date format on the unit.

•

When set, touch

menu.

Date

•

Touch

date.

•

Use the + and – keys to set the date values.

•

When the correct date has been set, touch OK

to return to the previous screen.

•

Touch

no changes are required.

Set Date

Cancel

Back

in order to enter the current

to return to the previous screen if

to return to the Settings

Time

•

Administrator Settings

Touch

using the 24 hour clock.

•

Use the + and – keys to set the time values.

•

When the correct time has been set, touch OK

to return to the previous screen.

•

Touch

no changes are required.

Date Format

Touch the format.

The options are dd/mm/yy or mm/dd/yy.

•

Touch

•

Touch

instrument settings.

This allows an administrator to remove passwords from password-protected programs, change the Administrator password and restore the unit to its factory settings.

•

Touch

password.

The default password is 1234.

•

Touch OK to enter the Administrator Settings

menu.

•

Touch

making any changes.

Remove Passwords

•

From the Administrator menu touch

Passwords

•

Touch the file with the password to be removed.

•

Touch

The password will now be removed and the program can be deleted if required.

•

Touch

Time

in order to enter the current time

Cancel

Back

Remove Password

to return to the previous screen if

Date

button to set the required date

to return to the Settings menu.

More Settings

None

and enter the administrator

Cancel

to return to the menu without

to return to the menu.

to access further

Remove

Change

Administrator Password

This allows the administrator to change the default password (1234).

•

From the Administrator menu touch

Administrator Password

•

Touch

password.

•

Touch the second

new password.

Note

keeps a record of the new password.

•

Touch OK to return to the menu or

return without making any changes.

Restore Factory Settings

All changes to the instrument defaults and settings can be removed and the unit returned to its factory settings.

•

From the Administrator menu touch

Factory Settings

•

Touch the

administrator password.

•

Touch

reset to factory defaults.

•

Touch

without making any changes.

None entered

: It is recommended that the administrator

None

Restore Settings

Cancel

to return to the Settings menu

and enter the new

None entered

button and enter the

and the unit will be

Change

to confirm the

Cancel

Restore

Heated Lid

Sample Cooling

Pause

Hot Start

PROGRAM DEFAULTS

The program default settings will apply to every new program created on the Prime Elite. However individual programs can be edited as required.

Use this to set the heated lid temperature to approximately 10ºC higher than the hottest block temperature in the program.

•

Touch

•

Touch the

required lid temperature (35 to 115ºC).

Preheat lid

Set to On to pre-heat the lid to the set temperature before the thermal cycling program begins.

•

Touch

Set to On to cool the block to 4ºC while the lid preheats.

•

Touch

•

Touch

On/Off

More Settings

to toggle between settings.

temperature

to toggle between settings.

button to set the

for further program defaults or

Back

to return to the Settings menu.

This will cause the unit to pause before the program starts at the ambient temperature of the block (unless Sample Cooling has been set to On; in this case the unit will pause with the block held at 4ºC) and the program will not commence until the unit is manually un-paused.

•

Touch

Initial Denaturation

Use this to denature the sample and/or activate the enzyme before the thermal cycling program begins.

•

Touch

•

Touch the

time.

•

Touch the

required temperature.

On/Off

to toggle between settings.

time

button to set the required hold

temperature

button to set the

This will cause the unit to pause at the set temperature. The program will not continue until the unit is manually un-paused.

•

Touch

•

Touch the

On/Off

to toggle between settings.

temperature

button to set the required temperature.

•

Touch

Final Extension

Final Hold

•

More Settings

for further program defaults or

Back

to return to the Settings menu.

Use this to add a final extension step to the program to ensure complete amplification of the products.

•

Touch

•

Touch the

time.

•

Touch the

required temperature.

This will hold the block at the set temperature at the end of a program until the user manually stops the unit.

•

Touch

•

Touch the

•

Touch

Settings menu.

SERVICE SETTINGS

The service settings are for the use of authorised service engineers only. A service engineer’s password is required to access these settings.

GRADIENT CALCULATOR

The Gradient Calculator can be used to calculate the temperature for each column of the block for a user-specified temperature and gradient range.

Touch the

the required temperature.

•

Touch OK.

•

Touch the

required gradient range followed by OK.

•

Touch OK.

The calculated temperatures for each of the columns of the block will now be displayed.

Note

paired as it is not possible to individually control 24 columns. Therefore only 12 column temperatures are available across this block.

On/Off

More Settings

to toggle between settings.

time

button to set the required hold

temperature

to toggle between settings.

temperature

button to set the

button to set the required temperature.

to return to the top of the Program Defaults menu or

target temperature

gradient button

: With the 384-well block, the columns are

button and enter

and enter the

Back

to return to the

•

OLIGO TM CALCULATOR

The oligo Tm calculator can be used to estimate the Tm of the PCR primers and help in selecting an appropriate annealing temperature for the thermal cycling program.

Touch

input the sequence of the primer.

•

Touch the

sequence.

•

Touch

•

Touch

•

Use the

sequence.

•

Touch OK to return to the calculator screen.

•

Enter the

•

Enter the

the PCR mix.

•

Touch

primer.

Enter The Oligo Sequence

A C G T

DEL

to delete the last character.

CLR

to delete the whole sequence.

arrow

keys to scroll through the

primer concentration

monovalent salt concentration

Calculate

to calculate the Tm of the

in order to

buttons to enter the primer

in the PCR mix.

•

To check the Tm of another primer with the same

primer and salt concentrations, touch enter the new primer sequence.

•

Touch

•

Touch

Once all the settings and defaults have been set as required, touch

return to the Home Screen.

•

If you do not wish to change any settings, touch

Reset

to clear the entries.

Back

to return to the Settings menu.

Back

and

Cancel

to return to the Home Screen.

Save

to save the changes and

CREATE A NEW PROGRAM

Home Screen

Create A New Program

From the can build a program by adding stages and steps and define the temperatures and hold times.

The function of each of the buttons on this screen is described below.

NAME THE PROGRAM

•

Touch this button to give the program a name.

•

Type in the required name using the text entry

screen.

•

Once the name is complete, touch OK to return

to the programming screen.

•

Touch

screen if no changes are required.

When saving the program, if the chosen name has already been used for another program, the message box shown opposite will appear.

•

Touch OK to return to the Programming screen.

•

Touch the

name.

•

Once the name is complete, touch OK to return

to the programming screen.

•

Touch

Cancel

program name

Save

to save the program.

, touch the

to return to the programming

and enter a different

button. This will open a screen where you

INSERT/DELETE A STAGE

stage

is a section of a program that consists of a number of individual steps that can be cycled

(repeated) a defined number of times.

Insert a new stage

•

To insert a stage in a program, touch

new stage

inserted.

Stage 1

•

Touch this to give the stage a name (optional).

Enter the name using the text entry screen.

1 cycle

•

Touch this to edit the number of cycles (repeats)

for the stage. Enter the required number of cycles then touch OK.

Temperature

•

Touch the

temperature of the step and add any increment/decrement or gradient features.

Time

•

Touch the

the step and to add any increment/decrement time features.

Ramp rate

•

Touch the

rate.

The range is 0.1ºC/s (minimum) to 5.0ºC/s maximum for heating and 3.0ºC/s for cooling. The value can be entered to 1 decimal place. Ramp rate will affect the heating or cooling rate from the previous step to the current step.

•

Once set, touch

Programming Screen.

Delete a stage

•

delete

highlight it and then touch

. A stage with one step will be

temperature

time

button to edit the hold time of

ramp rate

a stage, touch the

Insert a

button to edit the

setting to adjust the ramp

to return to the

Delete

stage area

INSERT/DELETE A STEP

Insert a new step

•

Touch

in the current stage.

•

Edit the new step temperature, hold time and

ramp rate as required.

Delete a step

•

To delete a step, touch the

highlight it and then touch

Insert a new step

INSERT/DELETE A PAUSE

Insert a pause

•

To insert a pause between stages, touch

a pause

The hold temperature will be that of the previous step. The unit will not continue with the remainder of the program until it is manually un-paused by the user.

Delete a pause

•

To delete a pause, touch the

highlight it and then touch

Note

programmed stages, it is necessary to first delete the later stages to where the pause is required.

: To insert a pause between two previously

PROGRAM OPTIONS

•

Touch this to view the program defaults as set

up in the Settings module.

Any of these parameters can be edited by touching the appropriate button and will be saved for the current program only.

•

Touch

screen.

Program

to return to the programming

to insert the next step

step area

Delete

pause

button to

Delete

Insert

User

User names can be entered and linked to the program to help in searching and identifying programs.

•

To enter a user name, touch the

button next to User.

•

User names that have already been created will

be listed.

•

To create a new user name, touch the

new user

•

Type in the user name and touch OK.

The new user name will be added to the list.

•

To select a user name, touch the button to

highlight it. Touch it again to de-select it.

•

To delete a user name from the list, select the

user name and touch

•

Touch

The user name will now appear in the Program Options screen.

•

Touch the

programming screen.

button.

Delete

Done

to return to Program Options.

Program

button to return the

None selected

Create a

Keywords

Keywords can be entered and linked to the program to help in searching for related programs.

•

To enter a keyword, touch the

button next to Keywords.

•

Other keywords that have already created will

be listed.

•

To create a new keyword, touch the

new keyword

•

Type in the keyword and touch OK.

The new key word will be added to the list.

•

To select a keyword, touch the button to

highlight it. Touch it again to de-select it. More than one keyword can be selected

•

To delete a keyword from the list, select the

keyword(s) and touch

•

Touch

The keyword(s) will now appear on the Program Options screen.

•

Touch the

programming screen.

Done

button.

Delete

to return to Program Options.

Program

button to return the

None selected

Create a

Passwords

A password can be created to protect the program from being edited and deleted by other users. The password will be requested if a user attempts to edit or delete the program.

•

To create a password, touch the

next to Password.

•

Enter the required password then touch OK. The

password is case-sensitive.

•

Touch

without entering a password.

•

Touch the

Programming screen.

•

To change or remove the password, from the

Program Options screen, touch the button next to Password.

•

On the Password Editing screen, touch the

None

•

To remove the password, touch

Password

•

To change the password, touch

Password

followed by OK.

•

Touch

screen if no change is required.

Cancel

button and enter the password.

Cancel

to return to Program Options

Program

and type in the new password,

to return to the Program Options

button to return to the

None

button

Remove

Change

Note

SETTINGS

•

Touch the

instrument defaults as set up in the Settings module.

Any of these parameters can be edited by touching the appropriate button and will be saved for the current program only.

•

Touch

screen.

Settings

Program

button to view the

to return to the programming

VIEW

•

Touch

View

to see a graphical representation of

the program.

•

If the program has more stages and steps that

are displayed on the screen, scroll through the program by dragging a finger across the touch screen.

The centre of the screen shows each temperature step and hold time (not to scale). Stages are indicated by a white bar spanning the steps within the stage.

The bottom of the screen shows the name of the program and the expected end time if it was started immediately.

: The program is not saved at this point.

•

Touch

Start

to start the program.

Note

: It is not possible to send the program to connected Satellite units from a view screen.

•

Touch

Edit

to edit the program.

•

Touch

Back

to return to the Programming Screen.

Note:

The temperature log will remain blank until the program has been started.

If a step has a gradient programmed into it, the bar will be shown thicker and indicate the upper and lower temperatures of the gradient range.

If a step has an incremented or decremented temperature, this will be indicated by a white spot next to the temperature.

If a step has an incremented or decremented time, this will be indicated by a white spot next to the time.

Gradient

Inc/dec

temperature

Inc/dec time

DELETE

This is used to delete an entry. First touch the required segment followed by

UP AND DOWN ARROWS

Use these buttons to scroll through a program.

SAVE

Touch this button to save the program.

CANCEL

Touch this button to cancel changes to the program and return to the Home Screen.

Delete

RUN A PROGRAM

Run a

From the Home Screen, touch one of the shortcuts to run one of the listed programs, or touch

Program

programs can be run directly or edited before running.

to search and select from other programs and templates saved on the unit. Selected

RUNNING A PROGRAM FROM THE SHORTCUT LIST

On the Home Screen, the Run a program area displays shortcuts to four programs. User Preferences in the Settings module can be used to select whether this displays the four most programs or the four most

•

To run one of the programs in the shortcut list,

touch the button to open the Pre-run Screen.

If there is one or more Satellite unit(s) connected to the Prime Elite, an additional screen will ask if you wish to run the program on a selected Satellite unit or the Prime Elite

•

Touch the required button to select.

•

Touch

If it is decided to run the program on a Satellite unit, an additional screen will allow selection of the required unit.

•

Touch to select the required Satellite unit.

•

Touch

correct Satellite unit has been selected. The selected Satellite unit will beep and flash its indicator lights.

•

Touch

•

Touch

Cancel

Identify

Send

Cancel

to return to the Home Screen.

if necessary to ensure the

to return to the previous screen.

frequently

used programs.

recently

used

Note

: If the user attempts to send a program to unit while it is already running, the warning screen shown opposite will be displayed.

•

Touch OK to exit.

THE PRE-RUN SCREEN

The Pre-run Screen shows a graphical representation of the program allowing for a rapid check of the temperature parameters.

The centre of the screen shows each temperature step and hold time (not to scale). Stages are indicated by a white bar spanning the steps within the stage.

The bottom of the screen shows the program name, the date and the expected end time if it was started immediately.

•

Touch

•

Touch

•

Touch

Start

to start the program.

Edit

to edit the program.

Back

to return to the previous screen.

Note:

The temperature log will remain blank until

the program has been started.

If a step has a gradient programmed into it, the bar will be shown thicker and indicate the upper and lower temperatures of the gradient range.

If a step has an incremented or decremented temperature, this will be indicated by a white spot next to the temperature.

If a step has an incremented or decremented time, this will be indicated by a white spot next to the time.

Gradient

Inc/dec

temperature

Inc/dec time

Adaptive ramp rate

Note

THE RUN SCREEN

The Run Screen will be displayed after a program has been started. It shows the instrument status and the progress of a run.

Once the program has been started, the timers in the top right hand corner of the screen will indicate how long the program has been running and the total run time. The progress bar at the top of the screen also indicates how far the run has progressed.

The status of the block is indicated by the step bars which display the activity of the block (i.e. ramping to temperature), paused or the count down time of the step.

Progress through the program is indicated by the stage bar(s) which show at which cycle of the program the unit is currently at.

The first time a program is run, the unit estimates the total run time based on the ramp rates set in the program. After the first cycle has completed the actual time taken to complete the cycle is then used to re-calculate the total run time. Subsequent runs of the same program will therefore have a more accurate estimate of total run time. The estimate also takes into account the ambient room temperature; therefore run times will also depend on ambient temperature.

•

Touch

ask you to confirm the stop.

•

Touch

temperatures. The previous two minutes of temperature readings can be viewed by scrolling across the screen using a finger. The complete log is written and saved on the unit.

Note

program status.

•

Touch

•

If paused, touch

program.

the drawer is closed.

Note

•

Touch

Once the run has finished,

Stop

to stop the program. A prompt will

Temperature log

: Touch

Pause

: If the drawer is opened while the unit is running, the program will pause and only resume when

: It is possible to access all modules of the software while the unit is running a program.

Back

Program View

to pause the program.

Continue

to return to the Home Screen and access other modules.

to view the block

to return to the

to un-pause the

Program Finished

will be displayed instead of End time.

Viewing a running program

from the Home

Screen:

Search from a list

Search the programs by k

eyword

•

Touch

•

Touch the area for the required instrument.

•

Touch

Current status

View

SEARCHING FOR A PROGRAM NOT IN THE SHORTCUT LIST

There are four ways to search for a program if it is not in the shortcut list:

•

Search from the complete list of saved programs.

•

Search by keyword.

•

Search by user.

•

Search by both keyword and user.

•

To search for a program saved on the unit,

touch Selection Screen.

•

Use the + and – buttons to scroll up and down

the list of stored programs.

•

Touch

programs in order. The options are:

•

Run a Program

Sort By Program Name

Program Name (ABC)/(ZYX) Recently Used (123)/(321) Most Used (123)/(321) User (ABC)/(ZYX)

to open the Program

to list the

•

Touch

the list of keywords stored on the unit.

•

Select one or more keywords by touching the

buttons to highlight them. Touch again to deselect.

•

When all keywords have been selected, touch

Done

Only the programs with the selected keyword(s) saved with them will be listed.

•

To clear the keyword search, touch

Keyword Search

Search by Keyword

to return to the Program selection screen.

and this will open

Clear

Search the programs by u

ser

•

Search the programs by

keyword and u

ser

Touch

of user names stored on the unit.

•

Select the required user name by touching the

appropriate button to highlight it. Touch again to de-select.

•

When the user name has been selected, touch

Done

Only the programs with the selected user name saved with them will be listed.

•

To clear the user name search, touch

User Search

•

Touch

the list of keywords stored on the unit.

•

Select one or more keywords by touching the

buttons to highlight them. Touch again to deselect.

•

When all keywords have been selected, touch

Done

Only the programs with the selected keyword(s) saved with them will be listed.

•

Touch

of user names saved with previously keywordsearched programs.

•

Select the required user name by touching the

appropriate button to highlight it. Touch again to de-select.

•

When the user name has been selected, touch

Done

Only the programs with both the selected user name and key word(s) saved with them will be listed.

Search by User

to return to the Program selection screen.

Search by Keyword

to return to the Program selection screen.

Search by User

to return to the Program selection screen.

and this will open the list

Clear

and this will open

and this will open the list

RUNNING A PROGRAM NOT ON THE SHORTCUT LIST

•

Once the required program has been identified,

touch it to highlight it.

The Program Selector Screen will show a summary of the program, the instrument settings and a graphical representation of the thermal cycling program.

•

Touch

•

Touch

•

Touch

If there is one or more Satellite unit(s) connected to the Prime Elite, an additional screen will ask if you wish to run the program on a selected Satellite unit or the Prime Elite

•

Touch the required button to select.

•

Touch

If it is decided to run the program on a Satellite unit, an additional screen will allow selection of the required unit.

•

Touch to select the required Satellite unit.

•

Touch

correct Satellite unit has been selected. The selected Satellite unit will beep and flash its indicator lights.

•

Touch

•

Touch

Edit

to edit the program if required.

Back

to return to the Home Screen.

Start

to proceed to the Pre-run Screen.

Cancel

Send

Cancel

to return to the Home Screen.

Identify

to return to the previous screen.

if necessary to ensure the

Note

: If the user attempts to send a program to unit while it is already running, the warning screen shown opposite will be displayed.

•

Touch OK to exit.

•

From the Pre-run Screen, touch

program.

See

THE RUN SCREEN

information on instrument status and progress during a run.

on page 48 for further

Start

to run the

EDITING A PROGRAM

•

EDITING SAVED PROGRAMS

First select the required program by searching the Run a program module.

To edit a selected program, touch the

button.

Any of the Program Options, Settings or thermal cycling parameters can be edited.

•

Once all the required changes have been

made, touch

Note: this will overwrite the existing program.

To copy a program for editing, please see section

VIEW FILES

EDITING TEMPLATES

Editing templates can be a quick method of creating a program by just changing a few key parameters. However it is not possible to save changes to the templates installed on the thermal cycler.

If the edited program is a template, touching will open the message box shown opposite.

•

Touch the

Name and enter a name for the edited program.

•

Touch

changes.

The re-named program will now be listed in the program list.

Save

None

button next to New Program

Save As New Program

to save the

Edit

Save

EDITING PASSWORD PROTECTED PROGRAMS

If the edited program is password protected, touching opposite. To save the changes either:

•

Touch the

Note: this will overwrite the existing program.

•

Touch the

The re-named program will now be listed as a separate program in the program list.

Save

will open the message box shown

None

button next to Password and

enter the correct password followed by

None

button next to New Program Name and enter a new name for the edited program, followed by

Save as a New Program

Save

CURRENT STATUS

Reset Satellite IDs

The Current Status area of the Home Screen provides status information on the Prime Elite and any connected Satellite units.

On the Home Screen, the Current status area shows the versions of software and firmware loaded on the unit.

•

Touch the

status of the Prime Elite and any connected units.

The Prime Elite and any satellite units connected to it will be listed. The ID shows the serial number of the unit in brackets and for Satellite units the ID number allocated in square brackets.

If a unit is running a program, as shown by the status, the Run Screen can be viewed. Runs can then be paused or stopped as required.

•

Touch the area to select the required unit.

•

Touch

•

Touch

When first connected, a Satellite unit is allocated an ID number by the Prime Elite. The ID numbers can be reset following the instructions given below.

•

Remove the USB cables from the rear of the

Satellite units.

•

Touch

•

During the next 120 seconds, re-insert the USB

cables in the order you wish the Satellite IDs to occur.

•

When done, touch OK.

Current status

View

Cancel

Reset Satellite IDs

to return to the Home Screen.

button to view the

VIEW FILES

Search from a list

Search the

files

by keyword

The View files module opens the File Manager and allows access to all the program and temperature log files. From here it is possible to copy program files to the unit for editing. Files can also be deleted or copied to a USB memory stick for transfer to a PC or storage.

FILE SEARCHING

There are four ways to search for a file:

•

Search program files or temperature logs from

the complete list of saved files.

•

Search by keyword.

•

Search by user.

•

Search by keyword and user.

•

To search for a file saved on the unit, touch

View files

•

Touch

programs only, temperature logs only or both.

•

Use the up and

through the list of saved files.

•

Touch

programs in order. The options are:

•

Touch

the list of keywords stored on the unit.

•

Select one or more keywords by touching the

buttons to highlight them. Touch again to deselect.

•

When all keywords have been selected, touch

Done

Only the files with the selected keyword(s) saved with them will be listed.

•

To clear the keyword search, touch

Keyword Search

to open the File Manager screen.

Programs And Logs

Sort By Program Name

Program Name (ABC)/(ZYX) Recently Used (ABC)/(ZYX) Most Used (ABC)/(ZYX) User (ABC)/(ZYX)

Search By Keyword

to return to the File Manager screen.

down arrow

and this will open

to display either

buttons to scroll

to list the

Clear

Search the

files by u

ser

•

Search the

files by keyword and u

ser

Touch

of user names stored on the unit.

•

Select the required user name by touching the

appropriate button to highlight it. Touch again to de-select.

•

When the user name has been selected, touch

Done

Only the programs with the selected user name saved with them will be listed.

•

To clear the user name search, touch

User Search

•

Touch

the list of keywords stored on the unit.

•

Select one or more keywords by touching the

buttons to highlight them. Touch again to deselect.

•

When all keywords have been selected, touch

Done

Only the programs with the selected keyword(s) saved with them will be listed.

•

Touch

of user names saved with previously keywordsearched files.

•

Select the required user name by touching the

appropriate button to highlight it. Touch again to de-select.

•

When the user name has been selected, touch

Done

Only the files with both the selected user name and key words saved with them will be listed.

Search By User

to return to the File Manager screen.

Search By Keyword

to return to the Program selection screen.

Search By User

to return to the File Manager screen.

and this will open the list

Clear

and this will open

and this will open the list

VIEWING A PROGRAM FILE

•

Touch

Programs.

•

Search for the required program file and touch it

to highlight it.

•

Touch

(Pre-run Screen) of the program.

•

Touch

Note

connected Satellite unit from the View screen.

•

Touch

•

Touch

Note

the program has been started.

Programs And Logs

View

to open the temperature profile

Start

to start the program.

: It is not possible to send a program to a

Edit

to edit the program.

Back

to return to the previous screen.

: The temperature log will remain blank until

VIEWING A TEMPERATURE LOG FILE

It is not possible to view a temperature log file on the Prime Elite. It must first be copied to a USB memory stick then viewed on a PC using the Techne Workbench software, which can be downloaded free of charge from www.techne.com

•

See the directions given in the next section for

copying files to a USB memory stick.

•

Copy the files to a PC and open the Workbench

software.

•

Use

Settings

files or click on files.

•

Select the required temperature log and click

Open

Note

: Since the .temperaturelogdata file is a text file, it can also be opened in applications such as Excel for further analysis.

to select the path for the saved

Open

to browse for the saved

to display Only

When opening a temperature log file, the Temperature Log Monitor screen will be displayed. To view the complete temperature log, drag the mouse across the screen to scroll through the data points.

The view will show a graphical representation of the actual temperature changes, second by second, throughout thermal cycling program.

Clicking on the will display the program that generated the temperature log.

For further information on using the Techne Workbench software, see the separate user manual which can be downloaded from www.techne.com

COPYING FILES

Files can either be copied onto the unit for editing, or to and from a USB memory stick.

To copy files to the unit:

•

Select one or more files for copying by touching

the appropriate file button.

Note

: Multiple file selection is optional and can be set up in the User Preferences section of the Settings Module.

•

Touch

•

Touch

A message box will confirm that the files have been copied.

The copied file(s) will now be highlighted and listed as Copy of… and can be edited as required.

Program View

Select All

Copy

button of this screen

to select all files.

To copy files to a USB memory stick:

•

Insert a USB memory stick into the port on the

front of the unit.

A message screen will appear confirming that the USB memory stick has been inserted.

•

Touch

Screen.

•

Select one or more files for copying by touching

the appropriate file button.

•

Touch

•

Touch

A message screen will appear giving the option of saving the files to the unit or to the USB memory stick.

•

Touch the upper button to save the file(s) to the

USB memory stick.

•

Touch

screen.

The file(s) will be saved on the USB memory stick in a folder called FileSystem as a .cyclerprogram file.

To copy files from a USB memory stick:

•

Insert a USB memory stick into the port on the

front of the unit.

A message screen will appear confirming that the USB memory stick has been inserted.

•

Touch

memory stick within the File Manager.

A USB button will be visible in the top right corner indicating that the files are being read from the USB memory stick.

•

Select one or more files for copying by touching

the appropriate file button.

•

Touch

•

Touch

A message screen will appear giving the option of saving the files to the unit or to the USB memory stick.

Cancel

Select All

Copy

Cancel

Yes

Select All

Copy

to return to the File Manager

to select all files.

to return to the File Manager

to view the programs on the USB

to select all files.

•

Touch the upper button to save the file(s) to the

Prime Elite unit.

•

Touch

screen.

DELETING FILES

Unused or old files can be permanently deleted from the unit or a USB memory stick.

•

Select one or more files for deleting by touching

the appropriate file button.

Note

set up in the User Preferences section of the Settings Module.

•

Touch

•

Touch

asking for confirmation of delete.

•

Touch

•

Touch

If the delete is confirmed, a message box will confirm that the files have been deleted.

•

Touch OK.

Cancel

to return to the File Manager

: Multiple file selection is optional and can be

Select All

Delete

Yes

Cancel

to select all files.

. A message box will appear

to confirm the delete.

to abort the delete.

UPDATING THE PRIME ELITE SOFTWARE

•

The Prime Elite software can be updated by downloading the latest version from the Techne website

www.techne.com. You will need a USB memory stick on which to save the update.

Note that the update tool requires at least Windows XP SP2 and the Microsoft .Net Framework 3.5 SP1, which, if needed, can be downloaded from:

http://www.microsoft.com/downloads/details.aspx?familyid=AB99342F-5D1A-413D-831981DA479AB0D7&displaylang=en

From the website, click on

Software Downloads

•

Select the

option to run the file.

•

Insert a USB memory stick into the PC and click

to select.

•

Click on

This will copy the update to the USB memory stick.

•

Turn the Prime Elite off and insert the USB stick

into the front USB port.

•

Turn the Prime Elite on and follow the

instructions on the screen.

The update will take several minutes. When finished, the home screen will be displayed.

•

Remove the USB memory stick from the Prime

Elite.

Prime Elite Update

Prepare the USB memory stick

Support

followed by

and choose the

FAQS

How do I adjust the pressure of the heated lid for my tubes or plates? No adjustment is necessary. The lid automatically applies the correct pressure. Ensure

that all consumables used in the block are of the same height.

Do I need a different block for fully skirted plates? No. The Prime Elite 96-well block will accommodate fully skirted as well as half-skirted and

non-skirted PCR plates.

What is “Sample Cooling”? Sample cooling can be applied during the lid pre-heat phase. This maintains the block at

4ºC while the lid is heating to temperature and avoids any increase in sample temperature due to the heated lid before the thermal cycling program begins.

How does TERS® work? TERS® harnesses the heat released during the cooling phase for use in the next heating

phase. By switching the fans off for a very short time at the end of the cooling period the heat is not removed from the heat sink, but is instead stored as heat energy. Heat is therefore available as soon as the Peltiers switch from cooling to heating. This results in faster heating, requires less energy to heat the block and subsequently costs less to run.

What is "Hot start"? The Hot Start step is used to pause the machine at a specific temperature, typically

around 70°C, after the initial template denaturation. The reason is to allow the manual addition of unmodified initial denaturation step. Heat-activated

What is the incremented time and temperature function for?

Incremented/decremented time and temperature are used to increase or decrease either the time or temperature incrementally over the number of cycles in a stage. Incrementation of extension time is used with 'Long PCR' which is when large template fragments are to be amplified (e.g. 27kb lambda DNA, 40kb genomic DNA). Decremented temperature is used for protocols such as 'Touchdown PCR' where the first cycle starts with a high annealing temperature and over the number of cycles in the stage there is a gradual decrease in the temperature. This ensures that only the specific product is amplified.

What is a gradient thermal cycler?

Gradient blocks enable a particular temperature step in a protocol to be programmed so that the temperature varies across the block. By specifying a temperature and the gradient to be applied, each column will achieve a different temperature. The set temperature is in the middle of the block, the lowest on the left and the highest on the right. The gradient is the total difference across the block, for example if the set temperature was 60°C and the gradient 10°C then the temperatures would range from approximately 55°C to 65°C from left to right.

Why is a temperature gradient required?

The annealing temperature of the specific primers used in DNA amplification often requires optimisation. Instead of running multiple experiments with different annealing temperatures the gradient thermal cycler allows the testing of up to 12 different annealing temperatures simultaneously.

Taq

DNA polymerase which may lose activity if added during the

Taq

or Hot Start enzymes do not require this step.

TECHNICAL SUPPORT AND SERVICING

If you require further technical or application assistance please contact Techne at:

E-mail: technehelp@bibby-scientific.com. Phone: +44 (0)1785 810433 Fax: +44 (0)1785 810405

For servicing information please contact:

Service Department Bibby Scientific Ltd. Beacon Road Stone Staffordshire ST15 0SA E-mail: service@bibby-scientific.com.

Phone: +44 (0)1785 810475 Fax: +44 (0)1785 810405

In the USA please contact service@techneusa.com or call +1 609 589 2560.

We are continually striving to improve our products and software. If you have any comments and suggestions on how we can do things better please send them to us at: technehelp@bibby-

scientific.com.

ADDITIONAL INFORMATION

USER MAINTENANCE

Before cleaning your unit, disconnect it from the power supply. The outer case of the Prime Elite may be cleaned with a cloth dipped in water or ethanol (hexane or 50% methanol can also be used). No part of the case or cover should be immersed in the solvents. Do not use aggressive solvents such as acetone or abrasive cleaners. The block may be wiped with water, ethanol or propan-2-ol and may be decontaminated by wiping with 2% Neutracon or 1% bleach solution.

Before using any cleaning or decontamination method except those recommended here, the responsible person should check with Techne that the proposed method will not damage the equipment.

FAULT FINDING

Note that this equipment should only be dismantled by properly trained personnel. Removing the outer cover exposes potentially lethal mains voltages. There are no user serviceable parts within this equipment.

Should you have any problems with your Prime Elite which cannot be easily remedied, you should contact your supplier and return the unit if necessary. Please include details of the fault observed and remember to return the unit in its original packing. Bibby Scientific Ltd. accepts no responsibility for damage to units which are not properly packed for shipping: if in doubt, contact your supplier, giving the full serial number of the unit.

FUSES

If the display on the front panel is not lit, one of the two fuses may have blown. Check that there is no external cause, such as a faulty plug or lead. Check both fuses and replace the faulty fuse with a new one of the correct value (fuse values are given on the label next to the power inlet). Note that fuses should only be replaced by a qualified electrician.

The holder for the two fuses is built into the mains input socket. First remove the power cable and then gently prise the fuse drawer open with a flat-bladed screwdriver or similar tool. Each fuse can be removed by using the screwdriver as a lever.

Exchange the faulty fuse in the fuse holder for a working fuse of the correct value. Finally, replace the fuse drawer in the fuse compartment and push the drawer shut. Fuses which blow repeatedly indicate a serious fault and you should return the unit to your supplier for repair.

INSULATION TESTING

This equipment is fitted with RFI suppression circuitry. Any check of the electrical insulation by means of high voltage dielectric testing (for example as in BS EN 61010-1) must be carried out using only a DC voltage.

This unit contains semiconductor components which may be damaged by electric field effects.

Product code

Description

REPLACEMENT PARTS

The following replacement parts can be obtained from Bibby Scientific Ltd. or your Techne distributor:

HH179(S) UK 230V mains lead with plug HH180(S) European mains lead with plug FCABLEUS US mains lead with plug 6205046 USB cable ELITE/M1 Operator’s Manual ELITE/02/B Prime Elite gradient block for 96 x 0.2ml micro tubes or plates ELITE/05/B Prime Elite gradient block for 60 x 0.5ml micro tubes ELITE/384/B Prime Elite gradient block for 384 well plates

Please note that replacement blocks can only be fitted by a trained service engineer.

DECLARATION OF CONFORMITY

Techne Prime Elite User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual