Techne Prime, PrimeG Operator's Manual

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Prime and PrimeG
OPERATOR’S MANUAL
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CONTENTS
PAGE
INTRODUCTION
3
BEFORE USE
4
Unpacking 4
SAFETY INFORMATION
5 English 5 Français 7 Deutsch 9 Italiano 11 Español 13
CONTACT INFORMATION
15
PRIME SPECIFICATION
16
INSTALLATION
18
GENERAL SOFTWARE FEATURES
20 Introduction 20 Template programs 20 Incremented/decremented time and temperature 21 Gradient (PrimeG only) 21 Oligonucleotide Tm calculator 22
PROGRAMMING TEXT, TEMPERATURE AND TIME
23 Text entry 23 Temperature entry 23 Time entry 27
INSTRUMENT SETTINGS AND DEFAULTS
30 User preferences 30 Instrument settings 31 Program defaults 33 Service settings 34 Gradient calculator 35 Oligonucleotide Tm calculator 35
CREATE A NEW PROGRAM
37 Name the program 37 Insert/delete a stage 38 Insert/delete a step 39 Insert/delete a pause 39 Program options 39 Settings 43 View 43 Delete 44 Up and down arrows 44 Save 44 Cancel 44
RUN A PROGRAM
45 Running a program from the shortcut list 45 The pre-run screen 45
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The run screen 46 Searching for a program not in the shortcut list 47 Running a program not on the shortcut list 49
EDITING A PROGRAM
50 Editing saved programs 50 Editing templates 50 Editing password-protected programs 51
CURRENT STATUS
52
VIEW FILES
53 File searching 53 Viewing a program file 55 Viewing a temperature log file 55 Copying files 56 Deleting files 58
UPDATING THE PRIME SOFTWARE
59
UPGRADING FROM PRIME TO PRIMEG
60
REMOVING AND EXCHANGING BLOCKS
61
FAQs
62
TECHNICAL SUPPORT AND SERVICING
63
ADDITIONAL INFORMATION
64 User Maintenance 64 Fault Finding 64 Fuses 64 Insulation Testing 64
ACCESSORIES
65
REPLACEMENT PARTS
65
DECLARATION OF CONFORMITY
66
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INTRODUCTION
The Prime is a stand-alone, flexible and versatile thermal cycler designed to accurately control the temperatures of samples between 4ºC and 100°C. It has many scientific applications including PCR1, reverse transcription, ligation and sequencing. It has user-interchangeable blocks to allow for most commonly used consumables including 0.2ml x 96 well plates, 0.2ml tubes, 0.5ml tubes and 384 well plates. The heated lid is fully adjustable to accommodate a wide range of different consumables.
The Prime is programmed via a 5.7” colour VGA LCD touch screen using simple and intuitive software. Programs and run progress can be viewed as a temperature/step graphical display, allowing the user to readily check the status or position within a run. Files can be saved with user names and/or keywords which allows for rapid searching and retrieval. A USB port on the front of the unit allows transfer of files to and from a USB memory stick for viewing in the Techne Workbench PC software and for the easy update of instrument software.
The Prime can easily be upgraded to the PrimeG which incorporates a block gradient function and gradient calculator, allowing for simple and rapid optimisation of reaction conditions.
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BEFORE USE
Before using the Prime please ensure you have read this manual carefully. If there is any doubt relating to the proper use of this equipment, the staff at Bibby Scientific Ltd. or your supplier will be happy to assist you.
UNPACKING
When unpacking the unit please ensure that the following have been removed from the packaging:
Prime with block
Mains cables (UK, EU and US)
Operator’s manual
Warranty card
The user is advised to keep the original packaging in case the instrument ever needs to be returned for service or repair. Bibby Scientific Ltd. accepts no responsibility for damage incurred unless the unit is correctly packed and transported in its original packaging.
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SAFETY INFORMATION
Please read all the information in this manual before using the Prime.
WARNING
HIGH TEMPERATURES ARE DANGEROUS: they can cause serious burns to operators and ignite combustible material. Users should be aware of the following potential hazards:
USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS
DO NOT use combustible substances near hot objects
DO NOT operate the instrument in the vicinity of inflammable liquids or gases
DO NOT place any liquid directly into the instrument.
OPERATOR SAFETY
All operators of Techne equipment must have available the relevant literature needed to ensure their safety. It is important that only suitably trained personnel operate this equipment, in accordance with the instructions contained in this manual and with general safety standards and procedures. If the equipment is used in a manner not specified by Bibby Scientific Ltd. the protection provided by the equipment to the operator may be impaired.
All Techne instruments are designed to conform to international safety requirements and are fitted with an over-temperature cut-out. If a safety problem should be encountered, switch off the unit at the mains socket and remove the plug from the electricity supply.
INSTALLATION
The instrument should be carried using both hands. Never move or carry the instrument when in use or connected to the mains electricity supply.
1. All Techne instruments are supplied with a power cable; this may be integral or plug-in.
2. Before connecting the instrument to the mains electricity supply, check the voltage
against the rating plate (located on the back of the unit). Please note that the unit must be earthed to ensure proper electrical safety. Connect the mains cable to a suitable plug according to the table below.
Connections 220/240V, 50/60Hz 110V/120V, 50/60Hz
Live Brown Black Neutral Blue White Earth Green/yellow Green
3. UK ONLY: The fused plug supplied with the mains cable is fitted with a 5 amp fuse to protect the
instrument and the operator.
4. The units are rated to operate at 100-240V, 50/60Hz.
5. Place the unit on a suitable flat bench or in a fume cupboard if required, ensuring that the air inlet
vents on the underside are free from obstruction.
6. Plug the mains cable into the socket on the back of the instrument.
7. Switch on the instrument using the switch located on the back of the unit.
Replacement cable
Should the mains lead need replacement, a cable of 1mm2 of harmonized code H05VV-F connected to an IEC320 plug should be used. IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN.
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ENVIRONMENTAL CONDITIONS
The Prime is designed operate under the following conditions:
Indoor use
Ambient temperature range +5ºC to +40ºC
Altitude to 2000m
Relative humidity not exceeding 80%
Mains supply fluctuations not exceeding 10%
Over voltage category II IEC 60364-4-443
Pollution degree 2
Note: The control specifications are quoted at an ambient temperature of 20ºC. The specification may deteriorate outside an ambient temperature of between 10ºC and 30ºC.
The instrument has been tested for radio frequency interference and is certified under EN61326.
WARRANTY
Bibby Scientific Ltd warrants this instrument to be free from defects in material and workmanship, when used under normal laboratory conditions for four (4) years. In the event of a justified claim, Bibby Scientific Ltd will replace any defective component or replace the unit free of charge. This warranty does not apply if damage is caused by fire, accident, misuse, neglect, incorrect adjustment or repair, damage by incorrect installation, adaption, modification, fitting of non-approved parts or repair by unauthorised personnel. Bibby Scientific Ltd liability is limited to the cost of repair or replacement of the product and excludes in particular, indirect and consequential loss, damage, costs or expenses, including but not limited to wasted time, materials and expenditure or loss of use, profit, production, revenue, expected savings or goodwill. To make a claim please contact the supplier of the instrument. This warranty is in addition to, and does not affect any statutory rights.
This manual has been prepared for the convenience of Techne’s customers and nothing in this manual shall be taken as a warranty, condition or representation concerning the description, merchantability, fitness for purpose or otherwise of the unit or components.
Notwithstanding the description and specification(s) of the instruments contained in the operator’s manual, Techne reserves the right to make such changes as it sees fit to the instruments or to any of the components.
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L'INFORMATION DE SÛRETÉ
Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le Prime.
AVERTISSEMENT
Les TEMPÉRATURES ÉLEVÉES SONT DANGEREUSES car elles peuvent provoquer de graves brûlures chez l’opérateur et enflammer les matériaux combustibles. Les utilisateurs doivent porter une attention toute particulière aux points suivants :
PROCEDER AVEC PRUDENCE ET PORTER DES GANTS POUR SE PROTEGER LES MAINS
NE PAS utiliser de matériaux combustibles auprès d’objets chauds.
NE PAS utiliser l’appareil à proximité de liquides ou de gaz inflammables
NE PAS verser de liquides directement dans l’appareil.
SECURITE DE L’OPERATEUR
Tous les utilisateurs de produits Techne doivent avoir pris connaissance des consignes et instructions nécessaires à la garantie de leur sécurité. Important: cet appareil doit impérativement être manipulé par un personnel qualifié et être utilisé selon les instructions données dans ce document, en accord avec les normes et procédures de sécurité générales. Dans le cas où cet appareil ne serait pas utilisé selon les consignes précisées par Bibby Scientific Ltd., la protection pour l’utilisateur ne serait alors plus garantie.
Tous les appareils Techne sont conçus pour répondre aux normes de sécurité internationales et sont dotés d’un coupe-circuit en cas de surchauffe. En cas de problème de sécurité, couper l’alimentation électrique au niveau de la prise murale et enlevez la prise connectée à l’appareil.
INSTALLATION
Porter l'appareil à deux mains. Ne jamais déplacer ou transporter l’appareil lorsqu’il est en fonctionnement ou branché à l’alimentation électrique.
1. Tous les appareils Techne sont livrés avec un câble d'alimentation, qui peut être intégré à l'appareil ou à raccorder.
2. Avant de raccorder l’appareil à l’alimentation électrique sur secteur, vérifier la tension
requise indiquée sur la plaque d’identification (située au dos de l’appareil). Il est
important que l’appareil soit relié à la terre pour assurer la protection électrique requise. Brancher le câble secteur sur une prise appropriée, voir tableau ci-après.
Connexions 220/240 V, 50/60 Hz 110/120 V, 50/60 Hz
Phase Marron Noir Neutre Bleu Blanc Terre Vert/jaune Vert
3. ROYAUME-UNI SEULEMENT: La prise avec fusible intégré fournie avec le câble secteur est munie d’un fusible 5A destiné à protéger l’appareil et l’utilisateur.
4. Les unités sont évaluées pour fonctionner à 100-240V, 50/60Hz.
5. Placer l’appareil sur une surface plane ou si nécessaire sous une hotte d’aspiration, veiller à ce que les trous d’aération situés sous l’appareil ne soient pas obstrués.
6. Raccorder le câble d’alimentation à la prise située à l’arrière de l’appareil.
7. Allumer l'appareil utilisant le commutateur situé à l'arrière de l'unité.
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Câble de rechange
S’il s’avère nécessaire de remplacer le cordon d’alimentation, utiliser un câble de 1 mm² conforme à la norme H05VV-F relié à une prise IEC320. EN CAS DE DOUTE, CONSULTER UN ELECTRICIEN
QUALIFIE.
CONDITIONS ENVIRONNEMENTALES
Le Prime est conçu pour fonctionner dans les conditions suivantes:
Pour un usage intérieur seulement
Température ambiante +5°C à +40°C
Altitude inférieure à 2000m
Humidité relative ne dépassant pas 80%
Fluctuations de l’alimentation n’excédant pas 10% de la valeur nominale
Catégorie II IEC 60364-4-443 de surtension
Degré de pollution 2
Remarque: Les paramètres sont indiqués pour une température ambiante de 20°C. Ces caractéristiques peuvent se détériorer en dehors d’une température ambiante de 10 à 30°C.
L’appareil a été testé en matière de radiofréquences et est certifié selon la norme EN61326.
GARANTIE
Bibby Scientific Ltd garantit cet instrument contre tout vice de matière et de fabrication, quand il est utilisé dans des conditions normales de laboratoire pendant quatre (4) ans. En cas de réclamation justifiée, Bibby Scientific Ltd procèdera gratuitement au remplacement de tout élément défectueux ou au remplacement de l'appareil. Cette garantie ne s'applique pas aux dommages provoqués par les incendies, accidents, mésusages, négligences, réglages ou réparations incorrects, aux dommages dus à une installation incorrecte, adaption, modification, au montage de pièces non homologuées ou réparation par un personnel non autorisé. La responsabilité de Bibby Scientific Ltd se limite aux coûts de réparation ou de remplacement du produit et exclut, en particulier, les pertes, dommages, coûts ou dépenses indirects et conécutifs, parmi lesquels, mais sans s'y limiter, les pertes de temps, de matériaux et les dépenses ou perte d'utilisation, de profit, de production, de chiffre d'affaires, d'économies escomptées ou écarts d'acquisition. En cas de réclamation, veuillez prendre contact avec le fournisseur de cet appareil. Cette garantie s'ajoute à vos droits légaux et n'affecte en rien ces derniers.
Le présent manuel a été exclusivement rédigé à l’attention des clients de la marque Techne et rien dans son contenu ne doit être pris comme une garantie, une condition ou une affirmation concernant la description, la commercialisation, l’adéquation à un usage particulier de l'appareil ou de ses composants.
Malgré la description et les caractéristiques techniques des appareils données dans le manuel de l’utilisateur, la société Techne se réserve le droit d’apporter les changements nécessaires à l’appareil ou à tout élément qui entre dans sa composition.
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SICHERHEITS- INFORMATIONEN
Lesen Sie diese Anleitung vor Verwendung des Prime bitte sorgfältig durch.
ACHTUNG
HOHE TEMPERATUREN STELLEN EINE GEFAHRENQUELLE DAR. Sie können schwere Brandverletzung verursachen und brennbare Stoffe entzünden. Der Benutzer sollt sich mit den möglichen Gefahren vertraut machen:
UMSICHTIG VORGEHEN UND SCHUTZHANDSCHUHE TRAGEN
KEINE brennbaren Stoffe in der Nähe heißer Gegenstände verwenden
Das Gerät NICHT in der Nähe entzündlicher Flüssigkeiten oder Gase betreiben
Flüssigkeiten NICHT direkt auf das Gerät auftragen.
SICHERHEIT DES BEDIENPERSONALS
Alle Benutzer von Techne Geräten müssen Zugang zu der entsprechenden Literatur haben, um ihre Sicherheit zu gewähren. Es ist wichtig, daß diese Geräte nur von entsprechend geschultem Personal betrieben werden, das die in dieser Gebrauchsanweisung enthaltenen Maßnahmen und allgemeine Sicherheitsbestimmungen und - vorkehrungen beachtet. Wenn das Gerät anders eingesetzt wird als vom Hersteller empfohlen, kann dies die persönliche Sicherheit des Anwenders beeinträchtigen.
Die Geräte von Techne entsprechen den internationalen Sicherheitsbestimmungen und sind mit einem automatischen Übertemperaturabschalter ausgestattet. Wenn ein Sicherheitsproblem auftreten sollte, muß das Gerät ausgeschaltet und vom Stromnetz getrennt werden.
INBETRIEBNAHME
Das Gerät mit beiden Händen tragen. Das Gerät unter keinen Umständen transportieren, wenn es in Betrieb ist, oder während das Gerät noch am Netz angeschlossen ist.
1. Alle Geräte von Techne werden mit einem Netzkabel geliefert, das entweder eingesteckt wird
oder fest mit dem Gerät verbunden ist.
2. Vor dem Anschluss bitte kontrollieren, ob die Stromversorgung den Angaben auf dem
Typenschild (auf der Geräterückseite) entspricht. Um die elektrische Sicherheit zu gewährleisten, muss dieses Gerät geerdet werden. Schließen Sie das Netzkabel entsprechend der folgenden Tabelle an einen geeigneten Stecker an.
Anschluss 220/240V, 50/60Hz 110V/120V, 50/60Hz
Phase Braun Schwarz Neutral Blau Weiß Erde Grün/Gelb Grün
3. NUR FÜR GROSSBRITANNIEN: der mit dem Netzkabel gelieferte Sicherungsstecker enthält
eine 5 Amp. Sicherung zum Schutz des Geräts und des Anwenders.
4. Die Maßeinheiten werden veranschlagen, um an 100-240V, 50/60Hz zu funktionieren.
5. Stellen Sie das Gerät auf einen geeigneten ebenen Tisch oder in einem Abzugsschrank auf und
sorgen Sie dafür, dass die Lufteinlassschlitze auf der Geräteunterseite nicht blockiert sind.
6. Stecken Sie das Netzkabel in die Buchse auf der Geräterückseite ein.
7. Schalten Sie das Instrument unter Verwendung des Schalters an, der auf der Rückseite der
Maßeinheit gelegen ist.
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Ersatzkabel
Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1 mm2 Adernquerschnitt und Europastecker (IEC 320) benötigt. IM ZWEIFELSFALL EINEN ELEKTRO-
FACHMANN HINZUZIEHEN.
UMWELTBEDINGUNGEN
Der Prime ist für den Einsatz unter folgenden Bedingungen ausgelegt:
Gebrauch in Innenräumen
Umgebungstemperatur zwischen +5ºC to +40ºC
Höhe: bis zu 2000 m
Relative Feuchte nicht über 80%
Netzspannungsschwankungen nicht über 10%
Überspannungsklasse 2 IEC 60364-4-443
Verschmutzungsgrad 2
Hinweis: Die Gerätespezifikationen beziehen sich auf eine Umgebungstemperatur von 20ºC und können sich außerhalb des Bereichs 10ºC bis 30ºC verschlechtern.
Das Gerät wurde auf HF-Störeinflüsse geprüft und entspricht den EMV-Bedingungen nach EN61326.
GARANTIE
Bibby Scientific Ltd gewährleistet, dass dieses Gerät vier (4) Jahre lang keine Material- oder Herstellungsfehler aufweist, wenn es unter normalen Laborbedingungen verwendet wird. In einem berechtigten Garantiefall wird Bibby Scientific Ltd alle defekten Komponenten oder das Gerät kostenlos austauschen. Diese Garantie gilt nicht, wenn der Schaden durch ein Feuer, einen Unfall, Missbrauch, Nachlässigkeit, falsche Einstellungen oder Reparaturen, falsche Installation, Adaptierung, Veränderung, den Einbau nicht zugelassener Teile oder die Reparatur durch nicht autorisiertes Personal verursacht wurde. Die Garantie von Bibby Scientific Ltd ist beschränkt auf die Kosten der Reparatur oder den Austausch des Produkts und schließt ausdrücklich indirekte und nachfolgende Verluste, Schäden, Kosten oder Ausgaben aus, einschließlich aber nicht beschränkt auf Zeit-, Material- und Geldverlust oder den Verlust des Gebrauchs, von Gewinn, Produktion, Einkommen, erwarteten Einsparungen und des Firmenwerts. Um einen Garantiefall zu melden, wenden Sie sich bitte an den Vertriebspartner des Geräts. Diese Garantie gilt zusätzlich zu und nicht anstelle von geltendem Recht.
Diese Anleitung wurde zur Information der Kunden von Techne erstellt und stellt in keinster Weise eine Gewährleistung, Bedingung oder Darstellung bezüglich der Beschreibung, Marktgängigkeit oder Zweckdienlichkeit dieser Geräte oder Bauteile dar.
Unabhängig von Beschreibung und Spezifikation(en) des hier beschriebenen Geräts behält sich Techne das Recht vor, Änderungen an diesem Gerät oder dessen Bauteilen vorzunehmen.
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INFORMAZIONI DI SICUREZZA
Leggere attentamente il presente manuale prima di usare il Prime.
AVVERTENZA
Le ALTE TEMPERATURE SONO PERICOLOSE in quanto possono provocare serie ustioni agli operatori e dare fuoco al materiale combustibile. Gli utenti devono conoscere i seguenti pericoli potenziali:
PRESTARE ATTENZIONE ED INDOSSARE GUANTI PROTETTIVI PER LE MANI
NON usare sostanze combustibili vicino ad oggetti caldi
NON mettere in funzione lo strumento nei pressi di liquidi o gas infiammabili
NON collocare alcun tipo di liquido direttamente nello strumento.
SICUREZZA DELL'OPERATORE
Il personale che utilizza l’apparecchiatura Techne deve avere a disposizione la documentazione necessaria al fine di assicurare la loro incolumità. È importante che solo personale adeguatamente addestrato utilizzi questo apparecchio, in conformità alle istruzioni contenute in questo manuale e nel rispetto delle normative e procedure generali di sicurezza. Se l’apparecchio è utilizzato in modo non specificato da Bibby Scientific Ltd., la protezione fornita dall’apparecchiatura all’utilizzatore potrebbe essere a rischio.
Tutte le unità Techne sono state progettate in conformità ai requisiti internazionali di sicurezza e sono equipaggiate con un interruttore anti surriscaldamento. Se si dovesse verificare qualche problema di sicurezza, disconnettere l’apparecchio dalla rete.
INSTALLAZIONE
Occorre trasportare lo strumento usando entrambe le mani. Non spostare né trasportare lo strumento quando è in funzione o collegato all’alimentazione elettrica di rete.
1. Tutti gli strumenti Techne sono forniti con un cavo di alimentazione; può essere integrale o plugin.
2. Prima di collegare lo strumento all'alimentazione elettrica di rete, controllare la
tensione confrontandola con la targhetta riportante i valori nominali (si trova sul retro dell'unità). Notare che al fine di garantire la corretta sicurezza elettrica, occorre che l'unità sia messa a terra. Collegare il cavo di rete ad una presa idonea secondo la tabella riportata alla pagina successiva.
Connessione 220/240V, 50/60Hz 110V/120V, 50/60Hz
Sotto tensione Marrone Nero Neutro Blu Bianco Terra Verde/giallo Verde
3. SOLO REGNO UNITO: la spina con fusibile fornita con il cavo di rete è dotata di un fusibile da 5 Amp per proteggere lo strumento e l'utente.
4. Le unità sono stimate funzionare a 100-240V, 50/60Hz.
5. Collocare l'unità su un banco piano idoneo o in una cappa aspirante se necessario, assicurandosi che gli sfiati delle prese d'aria nella parte inferiore non siano ostruiti.
6. Inserire il cavo di rete nella presa che si trova sul retro dello strumento.
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7. Accendere lo strumento per mezzo dell'interruttore situato sulla parte retro dell'unità.
Cavo di ricambio
Qualora occorra sostituire il cavo di rete, si dovrà utilizzare un cavo di 1mm2 codice armonizzato H05VV-F collegato ad una spina IEC 320. IN CASO DI DUBBIO, RIVOLGERSI A UN
ELETTRICISTA QUALIFICATO.
CONDIZIONI AMBIENTALI
Il Prime è stato progettato per funzionare nelle seguenti condizioni:
uso interno
range di temperatura ambiente da +5ºC a +40ºC
altitudine massima 2000 m.
umidità relativa non superiore all’80%
oscillazione dell’alimentazione di rete non superiore al 10%
categoria di sovratensione II IEC 60364-4-443
grado di inquinamento 2
Nota: le specifiche di controllo sono indicate ad una temperatura ambiente di 20ºC. Le specifiche potrebbero peggiorare fuori da una temperatura ambiente compresa tra 10ºC e 30ºC.
Lo strumento è stato collaudato per interferenze da radiofrequenze ed è certificato secondo la norma EN61326.
GARANZIA
Bibby Scientific Ltd garantisce che, per un periodo di quattro (4) anni e se utilizzato nelle normali condizioni di laboratorio, questo strumento non presenterà alcun difetto dei materiali o di fabbricazione. In caso di reclamo giustificato, Bibby Scientific Ltd sostituirà qualsiasi componente difettoso o rimpiazzerà l'unità in modo gratuito. Questa garanzia non si applica ai danni causati da incendio, incidenti, utilizzo improprio, negligenza, regolazioni o riparazioni scorrette, danni dovuti a installazione non corretta, adattamenti, modifiche o installazione di parti non approvate oppure riparazioni eseguite da personale non autorizzato. La responsabilità di Bibby Scientific Ltd è limitata al costo della riparazione o della sostituzione del prodotto ed esclude in particolare qualsiasi perdita indiretta o secondaria, danno, costo o spesa, ivi inclusi, a titolo di esempio e in modo non esaustivo, perdite di tempo, materiali e spese oppure perdite di utilizzo, profitto, produzione, ricavo, risparmio atteso o avviamento. Per esporre un reclamo, contattare il fornitore dello strumento. Questa garanzia è in aggiunta ai diritti di legge e non ha alcun effetto su di essi.
Il presente manuale è stato preparato ad uso dei clienti di Techne e niente di quanto in esso contenuto costituisce garanzia, condizione o rappresentanza riguardo la descrizione, la commerciabilità, l'idoneità allo scopo o altrimenti dell'unità o dei componenti.
Nonostante la descrizione e le specifiche dello strumento contenuti nel manuale dell'operatore, Techne si riserva il diritto di apportare le modifiche ritenute opportune agli strumenti o a qualsiasi loro componente.
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INFORMACIÓN DE SEGURIDAD
Lea atentamente este manual antes de utilizar el Prime.
ADVERTENCIA
LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que pueden ocasionar quemaduras graves a los operarios y prender el material combustible. Los usuarios deben conocer los posibles riesgos:
TENGA CUIDADO Y LLEVE GUANTES DE PROTECCIÓN PARA PROTEGERSE LAS MANOS
NO utilice sustancias combustibles cerca de objetos calientes
NO utilice el instrumento cerca de líquidos o gases inflamables
NO coloque un líquido directamente en el instrumento.
SEGURIDAD DEL OPERARIO
Todos los usuarios de equipos Techne deben disponer de la información necesaria para asegurar su seguridad. De acuerdo con las instrucciones contenidas en este manual y con las normas y procedimientos generales de seguridad, es muy importante que sólo personal debidamente capacitado opere estos aparatos. De no ser así, la protección que el equipo le proporciona al usuario puede verse reducida.
Todos los equipos Techne han sido diseñados para cumplir con los requisitos internacionales de seguridad y traen incorporados un sistema de desconexión en caso de sobre temperatura. En caso de que surgiera un problema de seguridad, desconecte el equipo de la red.
INSTALACIÓN
El instrumento se debe transportar con las dos manos. No mueva ni lleve el instrumento cuando se utilice o esté conectado al suministro eléctrico principal.
1. Todos los instrumentos Techne se suministran con un cable de alimentación, que puede ser integrado o ‘enchufable’.
2. Antes de conectar el instrumento al suministro eléctrico, compruebe que el voltaje
coincida con el indicado en la placa de régimen (situada en la parte trasera de la unidad). El instrumento debe disponer de una toma de tierra para garantizar la seguridad eléctrica adecuada. Conecte el cable de alimentación a un enchufe adecuado según la siguiente tabla.
Conexión 220/240V, 50/60Hz 110V/120V, 50/60Hz
Con corriente Marrón Negro Neutro Azul Blanco Toma de tierra Verde/amarillo Verde
3. SÓLO PARA EL REINO UNIDO: El enchufe suministrado con el cable de alimentación incluye un fusible de 5 amperios para ofrecer protección al instrumento y al usuario.
4. Las unidades se clasifican para funcionar en 100-240V, 50/60Hz.
5. Sitúe la unidad sobre una mesa plana o en una campana de laboratorio si es necesario, y asegúrese de que los orificios de ventilación situados en la parte inferior no tienen ninguna obstrucción.
6. Conecte el cable de alimentación en el enchufe situado en la parte trasera del instrumento.
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7. Encienda el instrumento usando el interruptor situado en la parte posterior de la unidad.
Cable de repuesto
Si es necesario sustituir el cable de alimentación, se debe utilizar un cable de 1mm2 de código armonizado H05VV, conectado a un enchufe IEC320. EN CASO DE DUDA, PÓNGASE EN
CONTACTO CON UN ELECTRICISTA.
CONDICIONES AMBIENTALES
El Prime está diseñado para utilizarse en las condiciones siguientes:
Uso en interior
Intervalo de temperatura ambiente +5ºC a +40ºC
Altitud: hasta 2000 m
Humedad relativa no superior al 80%
Fluctuaciones del suministro eléctrico no superiores al 10%
Categoría de sobrevoltaje II IEC 60364-4-443
Nivel de contaminación 2
Nota: Las especificaciones de control corresponden a una temperatura ambiental de 20ºC. Las especificaciones pueden empeorar si se utiliza el instrumento fuera del intervalo de temperatura comprendido entre 10ºC y 30ºC.
Se han realizado pruebas para comprobar la interferencia de radiofrecuencia del instrumento, el cual cumple la normativa EN61326.
GARANTÍA
Bibby Scientific Ltd garantiza que este equipo estará libre de defectos de materiales y mano de obra, si se utiliza en condiciones normales de laboratorio, durante un período de cuatro (4) años. En caso de reclamación justificada, Bibby Scientific Ltd sustituirá cualquier componente defectuoso o cambiará la unidad gratuitamente. Esta garantía no es aplicable si los daños han sido ocasionados por incendios, accidentes, usos inapropiados, negligencias, ajustes o reparaciones incorrectas, así como si se trata de daños ocasionados por la instalación, adaptación, modificación o incorporación en el instrumento de piezas no aprobadas o su reparación por personal no autorizado. La responsabilidad de Bibby Scientific Ltd se limita al coste de la reparación o sustitución del producto, y excluye específicamente pérdidas, daños, costes o gastos indirectos o accidentales, incluyendo pero sin estar limitado a ello, pérdidas de tiempo, materiales y gastos o pérdida de uso, beneficios, producción, ingresos, ahorros previstos u oportunidades comerciales. Si desea realizar una reclamación, póngase en contacto con el suministrador del equipo. Esta garantía complementa y no afecta a sus derechos legales.
Este manual se ha preparado con una finalidad informativa para los clientes de Techne y ninguna parte del manual se deberá considerar como una garantía, condición o reflejo con respecto a la descripción, comerciabilidad, idoneidad para un fin determinado o de otro tipo de la unidad o sus componentes.
Con independencia de la descripción y las especificaciones del instrumento que se indican en el manual del operario, Techne se reserva el derecho de realizar cambios en el instrumento o en cualquiera de sus componentes cuando lo estime oportuno.
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15
CONTACT INFORMATION
For technical, sales or servicing information, contact your local Techne dealer or:
UK
Bibby Scientific Ltd. Beacon Road Stone Staffordshire ST15 0SA UK Tel: +44 (0)1785 812121 Fax: +44 (0)1785 810405 E-mail: sales@bibby-scientific.com
www.techne.com
North and South America
Bibby Scientific US Inc. t/a Techne Inc. 3 Terri Lane, Suite 10 Burlington, N.J. 08016 USA Toll Free (in NA): 800-225-9243 Tel: +1 609 589 2560 Fax: +1 609 589 2571 E-mail: labproducts@techneusa.com
www.techneusa.com
France
Bibby Scientific Limited Bâtiment Le Deltaparc Parc Silic PN2 7 rue du Canal BP 55437 VILLEPINTE 95944 ROISSY Charles de Gaulle France Tel: +33(0)148 63 78 03 Fax: +33(0)148 63 78 01 E-mail: ventes@bibby-scientific.com
www.bibby-scientific.com
Italy
Bibby Scientific Italia Srl Via Alcide de Gasperi 56 20070 Riozzo di Cerro al Lambro (MI) Italia Tel: +39 (0)2 98230679 Fax: +39 (0)2 98230211 E-mail: marketing@bibby-scientific.it
www.bibby-scientific.it
Middle East
Bibby Scientific Middle East Ltd. PO Box 27887, Engomi 2433 Nicosia Cyprus Tel: +357 22 660 423 Fax: +357 22 660 424 e-mail: sales@bibby-scientificme.com
Asia
Bibby Scientific - Singapore Prudential Tower, Level 26 30 Cecil Street Singapore 049712 Tel: +65 6631 2976 Fax: +44 (0) 1785 810405 e-mail: info@bibby-scientific.com
www.bibby-scientific.com
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16
PRIME SPECIFICATION
TEMPERATURE
Block temperature range 4ºC (10°C during cycling) to 100ºC Block uniformity (at 55ºC) <±0.3ºC Temperature accuracy (at 55ºC) <±0.25ºC Gradient availability (PrimeG only) Between 30ºC and 80ºC Maximum gradient (PrimeG only) 29ºC (19ºC with the 384-well block) Minimum gradient (PrimeG only) 1ºC Column uniformity with a 10ºC gradient
(PrimeG only)
<±0.3ºC
Pre-run sample cooling Yes, 4ºC Temperature set point adjustment 0.1ºC
HEATING/COOLING RATE
Maximum heating rate 3.4ºC/s Minimum heating rate 0.1ºC/s Maximum cooling rate 1.0ºC/s Minimum cooling rate 0.1ºC/s
HEATED LID
Selectable heated lid temperature 35 to 115ºC or off Lid uniformity ±5ºC Pre-heat lid Yes Warm up time from ambient <2 min Over-temperature cut-out Yes Regulated heated lid pressure Manually adjustable
The heated lid is only operational if the block temperature is set above 35ºC
PROGRAMMING
Program interface 5.7” colour VGA LCD touch screen Maximum number of programs stored 1000 Maximum number of stages per program 10 Maximum number of steps 10 Maximum number of cycles per stage 99 Programmable ramp rate Yes, 0.1ºC/s steps Maximum hold time 4h 59m 59s Minimum hold time 1s Gradient calculator (PrimeG only) Yes Incremented/decremented temperature Yes Incremented/decremented time Yes Pause facility Yes Program naming Alpha numeric plus symbols Password protection Yes Keyword selection Yes Run completion time Yes End of program alarm Yes Auto resume on power failure Yes, always or never Oligonucleotide Tm calculator Yes, based on the Nearest-Neighbour method1 Software updates Free of charge from www.techne.com
1
Breslauer, K.J.; Frank, R.; Blocker, H. and Marky, L.A. (1986) Proc. Natl. Acad. Sci. USA 83, pp
3746-3750.
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17
COMMUNICATION PORTS
USB port on front of unit Connection for USB memory stick USB port on rear of unit Connection for servicing and calibration only
DIMENSIONS
Height (mm) 240 Width (mm) 240 Length (mm) 420 Weight (kg) 9.4
POWER
Voltage 100-240V, 50/60Hz Power 425W
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18
INSTALLATION
See also the Safety Information sections on pages 5 to 14.
Front view
1. Lid adjustment knob.
2. Lid release latch.
3. 5.7” colour VGA LCD touch screen display.
4. USB port for memory stick.
Rear view
1. Mains cable inlet.
2. On/Off rocker switch
3. USB port for service and calibration only.
Installation
1. Place the unit on a suitable flat bench, ensuring that
the air inlet vents on the sides are free from obstruction.
2. Insert the block into the unit ensuring the connectors
on the block and unit are aligned.
3. Slide the quick release bars on the block towards the
closed padlock symbols to lock the block in place.
4. Plug the mains cable into the socket on the back of the
instrument.
5. Connect to the mains electricity supply with the plug
provided or one wired correctly for the supply. Switch the power ON using the switch located on the rear of the unit. The front display will then light up.
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19
6. Release the heated lid by lifting the latch forward
and use it to lift the lid. To close the lid, lower the lid onto the block and press the latch to secure in place.
7. Place the samples in the block. If individual tubes or
strip tubes are being used, space these out evenly across the block to equalise the pressure from the heated lid.
8. The heated lid has a rotating knob to adjust the lid
pressure on the samples, allowing for a variety of consumables to be used. To adjust the pressure:
a. Place the samples in the block and place the lid
in the down position.
b. Adjust the orange rotating knob anticlockwise to
give the least pressure then close the lid fully.
c. Gently rotate the knob clockwise until you can
feel pressure beginning to be applied.
d. Rotate the knob a further quarter of a turn and
this will give the correct pressure. Do not over­tighten.
9. Once the pressure has been set it does not need to be adjusted unless a different block or
consumable is used.
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20
GENERAL SOFTWARE FEATURES
INTRODUCTION
The Prime software allows the user to quickly and easily create thermal cycling programs using a simple and clear touch screen format. The software is structured into five modules which are accessed from the Home Screen. These allow the user to:
Run an existing program, selecting from templates or programs saved on the unit.
Create a new program.
Set instrument and program defaults and
preferences. Includes calculator tools.
View the current status of the unit.
View and search for files stored on the unit.
TEMPLATE PROGRAMS
To help with programming, a number of program templates are installed on the unit which are available to copy and edit or can be run directly without changes. Details of the installed program templates are given below.
Instrument and program defaults
Parameter
2 Step
Template
3 Step
Template
RT-
PCR Template
Heated Lid 105ºC 105ºC 105ºC Heated lid before program On On On Sample cooling On On On Pause before program Off Off Off Initial denaturation 94ºC, 05m00s 94ºC, 05m00s 94ºC, 05m00s Hot start Off Off Off Final extension 72ºC, 05m00s 72ºC, 05m00s 72ºC, 07m00s Final hold 10ºC 10ºC 10ºC
Thermal cycling conditions
2 Step
Template
3 Step
Template
RT-
PCR Template
Stage
1
Number of cycles 30 30 1 Step 1 94.0ºC, 00m30s 94.0ºC, 00m30s 45.0ºC, 40m00s Step 2 60.0ºC, 01m00s 55.0ºC, 00m30s 94.0ºC, 05m00s Step 3 72.0ºC, 00m30s
Stage
2
Number of cycles 40 Step 1 94.0ºC, 01m00s Step 2 55.0ºC, 00m50s Step 3 72.0ºC, 01m00s
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21
The Ice Bucket and Ligation programs are simple temperature holds, with the former having an infinite hold at 10ºC and the latter at 15ºC. In both of these programs the heated lid is switched off and the initial denaturation, hot start and final extension functions disabled.
INCREMENTED/DECREMENTED TIME AND TEMPERATURE
The software allows the user to either increment or decrement the time and/or temperature of a step within a cycling stage. These features are used with applications such as touchdown PCR where the annealing temperature is gradually decreased during the cycling process and long PCR, where due to the size of the product, long annealing/extension times are required and the extension time is increased by 15 to 20s per cycle during the final stages of the reaction.
GRADIENT (PRIMEG ONLY)
The gradient feature of the PrimeG can be useful in optimising the annealing conditions for primers. A gradient can be set around a temperature in any step of a program. The set temperature is the temperature in the middle columns of the block and the gradient is the variation at the two extremes of the block; the left hand columns being the coolest and the right hand columns the hottest.
The maximum temperature gradient range which can be set is 29ºC and the minimum is 1ºC; the lowest temperature is 30ºC and the hottest temperature is 80ºC. Examples are given in the table below:
Lowest temperature
(ºC)
Set temperature
(ºC)
Highest temperature
(ºC)
Gradient
(ºC)
50 55 60 10
40.5 55 69.5 29
50.5 65 79.5 29
A gradient calculator is included in the Settings module so the user can obtain an accurate estimation of the temperature of each column during the gradient stage. The temperatures for any particular gradient are calculated from an algorithm stored in the block firmware.
A B C D E F G H
1 2 3 4 5 6 7 8 9 10 11 12
Set
temperature
Lowest
temperature
Highest
temperature
Gradient
A B C D E F G H
1 2 3 4 5 6 7 8 9 10 11 12
A B C D E F G H
1 2 3 4 5 6 7 8 9 10 11 12
Set
temperature
Lowest
temperature
Highest
temperature
Gradient
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22
OLIGONUCLEOTIDE TM CALCULATOR
One of the most important parameters in a PCR is the annealing temperature of the reaction. This can be determined theoretically using various software programmes to calculate the melting temperature (Tm) of the primers.
The Tm is defined as the temperature at which half the DNA strands are single stranded and half are double stranded when base paired to a complimentary strand. Using annealing temperatures that are well below the Tm of the primers can result in mismatching, false priming and may lead to primer-dimer artefacts. Annealing temperatures that are significantly higher than the primer Tm may result in reduced priming or prevent priming altogether.
The Prime uses the Nearest-Neighbour method for calculating the Tm of an oligonucleotide1.
The Tm is calculated using the following equation:
Tm =
{(∆Hº x 1000)/(A + ∆Sº + R ln(C/4))} - 273.15 + 16.6 x log[Na+]
Where:
H
º
= The sum of the Nearest-Neighbour enthalpy changes (Kcal/mol)
A = Helix initiation correction constant
S
º
= The sum of the Nearest-Neighbour entropy changes (cal K
-
1
mol
-
1
)
R = The Gas Constant (1.99 cal K
-
1
mol
-
1
) C = Concentration of the oligo (M) [Na+] = Concentration of monovalent ions (M)
1
Breslauer, K.J.; Frank, R.; Blocker, H. and Marky, L.A. (1986) Proc. Natl. Acad. Sci. USA 83, pp
3746-3750.
The Oligonucleotide Tm calculator can be found in the
Settings
module where the user can input the oligonucleotide sequence, its concentration in the reaction and the salt concentration of the reaction mix.
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23
PROGRAMMING TEXT, TEMPERATURE AND TIME
This section gives instructions for the basic programming of text, temperature and time which are required in later sections.
TEXT ENTRY
When giving a name to a program or stage, or when entering a keyword or a password, a text entry screen will be opened.
Touch individual characters to create the name.
For words with all upper case letters touch
CAPS
.
Touch
SHIFT
if only the first letter is required in
upper case.
For numbers touch
123
.
For commonly used symbols touch
SYM
.
Touch the
space bar
(not labelled) for spaces
between characters.
To clear the typed name, touch
CLR
.
To delete the last character touch
DEL
.
Once the name is complete, touch OK.
TEMPERATURE ENTRY
When touching a temperature parameter a temperature entry screen will open. There are two forms of temperature entry screen:
A
simple
form for adjusting the temperature of the instrument parameters such as the heated lid
and program parameters such as initial denaturation and final extension.
An
advanced
form when programming step temperatures within a cycling stage that includes the options for introducing an incremented/decremented step temperature, for example in touchdown PCR, or a temperature gradient.
Simple temperature entry
Enter the temperature, type in the required value.
The decimal point can be used for 0.1ºC resolution.
To clear the entry press
DEL
.
If an entered temperature is invalid, a prompt will
indicate the acceptable range.
Once the temperature is correct, touch OK to return
to the previous screen.
Touch
Cancel
to return to the previous screen if no
changes are required.
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24
Advanced temperature entry
First ensure the number of cycles for the stage has been set then enter the required hold
temperature as described above.
Note
: Increase/Decrease Per Cycle will be inactive unless the number of cycles set is greater than 1.
To
increase the
step
temperature
per cycle
:
For an
increase
in temperature per cycle, touch
the button below
Increase Per Cycle
to turn this function on. Next touch the temperature entry button below this.
A new screen will open which is similar to the simple temperature entry screen. The maximum increase in temperature per cycle (based on the number of cycles and the temperature limits of the unit) will be shown.
Enter the required value.
Touch
DEL
to clear the entry.
If an entered incremented temperature is invalid, a
prompt will indicate the acceptable range.
Touch OK to accept and return to the previous
screen.
The increase in temperature per cycle will now be displayed.
Touch OK again to return to the programming
screen.
Touch
Cancel
to return to the programming screen
if no changes are required.
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25
The temperature value will now appear orange in the programming screen indicating that it contains a modified function.
To
decrease the
step
temperature
per cycle
:
For a
decrease
in temperature per cycle, touch the
button below
Decrease Per Cycle
to turn this function on. Next touch the temperature entry button below this.
A new screen will open which is similar to the simple temperature entry screen. The maximum decrease in temperature per cycle (based on the number of cycles and the temperature limits of the unit) will be shown.
Enter the required value.
Touch
DEL
to clear the entry.
If an entered decremented temperature is invalid, a
prompt will indicate the acceptable range.
Touch OK to return to the previous screen.
The decrease in temperature per cycle will now be displayed.
Touch OK again to return to the programming
screen.
Touch
Cancel
to return to the programming screen
if no changes are required.
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26
The temperature value will now appear orange in the programming screen indicating that it contains a modified function.
Note:
It is not possible to both increase and decrease
the temperature of the same step.
To enter a temperature gradient
on a step (
Prime
G
only):
On the PrimeG an additional
Temperature Gradient
button is available.
Touch the button below
Temperature Gradient
to turn this function on. Next touch the temperature entry button below this.
A new screen will open which is similar to the simple temperature entry screen. The maximum temperature gradient (based on the set hold temperature and the temperature limits of the unit) will be shown.
Enter the required value.
Touch
DEL
to clear the entry.
If an entered gradient temperature is invalid, a
prompt will indicate the acceptable range.
Touch OK to return to the previous screen. The
temperature gradient for the step will now be displayed.
Touch OK again to return to the programming
screen.
Touch
Cancel
to return to the programming screen
if no changes are required.
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27
The temperature value will now appear orange in the programming screen indicating that it contains a modified function.
TIME ENTRY
When touching a time parameter a time entry screen will open. There are two forms of time entry screen:
A
simple
form for adjusting the time of the program parameters such as initial denaturation and
final extension.
An
advanced
form when programming step hold times within a cycling stage that includes the
options for introducing incremented/decremented time, for example in long PCR.
Simple time entry
Enter the time using the + or – buttons to increase
or decrease the number of hours, minutes or seconds.
Using the – button with the value set at 0 will scroll
to the maximum permitted value.
Touching
Hide Hours
will remove the hour time selector and just display selectors for minutes and seconds.
Once the time is correct, touch OK to return to the
previous screen.
Touch
Cancel
to return to the previous screen if no
changes are required.
Advanced time entry
First ensure the number of cycles for the stage has been set then enter the required hold time as
described above.
Note: Increase/Decrease Per Cycle
will be inactive unless the number of cycles set is greater than
1.
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28
To
increase the
step hold time per cycle
:
For an
increase
in hold time per cycle, touch the
button below
Increase Per Cycle
to turn this function on. Next touch the time entry button below this.
A new screen will open which is similar to the simple time entry screen. The maximum increase in hold time per cycle (based on the number of cycles and the time limits of the unit) will be shown.
Enter the required value using the + and - buttons.
If an entered incremented time is invalid, a prompt
will indicate the acceptable range.
Touch OK to return to the previous screen.
The increase in hold time per cycle will now be displayed.
Touch OK again to return to the programming
screen.
Touch
Cancel
to return to the programming screen
if no changes are required.
The time value will now appear orange in the programming screen indicating that it contains a modified function.
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29
To
decrease the
step hold time per cycle
:
For a
decrease
in hold time per cycle, touch the
button below
Decrease Per Cycle
to turn this function on. Next touch the time entry button below this.
A new screen will open which is similar to the simple time entry screen. The maximum decrease in hold time per cycle (based on the number of cycles and the time limits of the unit) will be shown.
Enter the required value.
If an entered decremented time is invalid, a prompt
will indicate the acceptable range.
Touch OK to return to the previous screen.
The decrease in hold time per cycle will now be displayed.
Touch OK again to return to the programming
screen.
Touch
Cancel
to return to the programming screen
if no changes are required.
The time value will now appear orange in the programming screen indicating that it contains a modified function.
Note:
It is not possible to both increase and decrease
the time of the same step.
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30
INSTRUMENT SETTINGS AND DEFAULTS
Touch the
Settings
button to access the instrument Settings menu.
Touch any of the buttons to view and edit the
settings.
Touch
More Settings
to access further options.
Once all the settings and defaults have been set as
required, touch
Save
to save the changes and
return to the Home Screen.
If you do not wish to change any settings, touch
Cancel
to return to the Home Screen.
USER PREFERENCES
Language
Touch the language button to set the required
language. The options are English, French, Spanish, Italian and German.
Temperature Display
Touch the temperature button to set the required
temperature units. The options are ºC and ºF.
Home Screen File List Sort
This sets the user preference for listing the program shortcuts on the Home Screen.
Touch the button to set the required sorting
preference. The options are recently used first or most used first.
Touch
More Settings
for further user preferences.
Select Multiple Items in File Manager
This allows either multiple files or only single files to be selected for copying and deleting when using the File Manager in the View files module.
Touch to select On or
Off
.
Touch
Back
to return to the Settings menu.
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31
INSTRUMENT SETTINGS
Temperature Log Recording
To record the temperature logs (block temperature profile) of each run, touch the button to set this to Always. If temperature logs are not required, set to Never.
Auto Resume On Power Loss
This determines whether the unit will restart after a power failure. Setting Auto Resume to Always will always allow the unit to re-start irrespective of the length of the power failure.
Date and Time Settings
Use these settings to set the current date, time and date format on the unit.
When set, touch
Back
to return to the Settings
menu.
Date
Touch
Set Date
in order to enter the current date.
Use the + and – keys to set the date values.
When the correct date has been set, touch OK to
return to the previous screen.
Touch
Cancel
to return to the previous screen if no
changes are required.
Time
Touch
Time
in order to enter the current time using
the 24 hour clock.
Use the + and – keys to set the time values.
When the correct time has been set, touch OK to
return to the previous screen.
Touch
Cancel
to return to the previous screen if no
changes are required.
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32
Date Format
Touch the date button to set the required date format.
The options are dd/mm/yy or mm/dd/yy.
Touch
More Settings
for further date and time
settings or
Back
to return to the Settings menu.
Administrator Settings
This allows an administrator to remove passwords from password-protected programs, change the Administrator password and restore the unit to its factory settings.
Touch
None
and enter the administrator password.
The default password is 1234.
Touch OK to enter the Administrator Settings
menu.
Touch
Cancel
to return to the menu without making
any changes.
Remove Passwords
From the Administrator menu touch
Remove
Passwords
.
Touch the file with the password to be removed.
Touch
Remove Password
.
The password will now be removed and the program can be deleted if required.
Touch
Close
to return to the menu.
Change Administrator Password
This allows the administrator to change the default password (1234).
From the Administrator menu touch
Change
Administrator Password
.
Touch
None
entered and enter the new password.
Touch the second
None
entered to confirm the new
password.
Note
: It is recommended that the administrator keeps
a record of the new password.
Touch OK to return to the menu or
Cancel
to return
without making any changes.
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33
Restore Factory
Settings
All changes to the instrument defaults and settings can be removed and the unit returned to its factory settings.
From the Administrator menu touch
Restore
Factory Settings
.
Touch the
None
button and enter the administrator
password.
Touch
Restore Settings
and the unit will be reset
to factory defaults.
Touch
Cancel
to return to the Settings menu
without making any changes.
PROGRAM DEFAULTS
The program default settings will apply to every new program created on the Prime. However individual programs can be edited as required.
Heated Lid
Use this to set the heated lid temperature to approximately 10ºC higher than the hottest block temperature in the program.
Touch
On/Off
to toggle between settings.
Touch the temperature button to set the required lid
temperature (35ºC to 115ºC).
Preheat lid
Set to On to pre-heat the lid to the set temperature before the thermal cycling program begins.
Touch
On/Off
to toggle between settings.
Sample Cooling
Set to On to cool the block to 4ºC while the lid preheats.
Touch
On/Off
to toggle between settings.
Touch
More Settings
for further program defaults
or
Back
to return to the Settings menu.
Pause
This will cause the unit to pause before the program starts at the ambient temperature of the block unless Sample Cooling has been set to On. In this case the unit will pause with the block held at 4ºC and the program will not commence until the unit is manually un-paused.
Touch
On/Off
to toggle between settings.
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34
I
nitial Denaturation
Use this to denature the sample and/or activate the enzyme before the thermal cycling program begins.
Touch
On/Off
to toggle between settings.
Touch the time button to set the required hold time.
Touch the temperature button to set the required
temperature.
Hot Start
This will cause the unit to pause at the set temperature. The program will not continue until the unit is manually un-paused.
Touch
On/Off
to toggle between settings.
Touch the temperature button to set the required
temperature.
Touch
More Settings
for further program defaults
or
Back
to return to the Settings menu.
Final Extension
Use this to add a final extension step to the program to ensure complete amplification of the products.
Touch
On/Off
to toggle between settings.
Touch the time button to set the required hold time.
Touch the temperature button to set the required
temperature.
Final Hold
This will hold the block at the set temperature at the end of a program until the user manually stops the unit.
Touch
On/Off
to toggle between settings.
Touch the temperature button to set the required
temperature.
Touch
More Settings
to return to the top of the
Program Defaults menu or
Back
to return to the
Settings menu.
SERVICE SETTINGS
The service settings are for the use of authorised service engineers only. A service engineer’s password is required to access these settings.
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35
GRADIENT CALCULATOR (PRIMEG ONLY)
The Gradient Calculator can be used to calculate the temperature for each column of the block for a user-specified temperature and gradient range.
Touch the
Target Temperature
button and enter
the required temperature.
Touch OK.
Touch the
Temperature Gradient
button and enter
the required gradient range followed by OK.
Touch OK.
The calculated temperatures for each of the columns of the block will now be displayed.
Note
: With the 384-well block, the columns are paired as it is not possible to individually control 24 columns. Therefore only 12 column temperatures are available across this block.
OLIGO TM CALCULATOR
The oligo Tm calculator can be used to estimate the Tm of the PCR primers and help in selecting an appropriate annealing temperature for the thermal cycling program.
Touch
Enter The Oligo Sequence
in order to input
the sequence of the primer.
Touch the
A C G T
buttons to enter the primer
sequence.
Touch
DEL
to delete the last character.
Touch
CLR
to delete the whole sequence.
Use the arrow keys to scroll through the sequence.
Touch OK to return to the calculator screen.
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36
Enter the primer concentration in the PCR mix.
Enter the monovalent salt concentration of the PCR
mix.
Touch
Calculate
to calculate the Tm of the primer.
To check the Tm of another primer with the same
primer and salt concentrations, touch
Back
and
enter the new primer sequence.
Touch
Reset
to clear the entries.
Touch
Back
to return to the Settings menu.
Once all the settings and defaults have been set as required, touch
Save
to save the changes and
return to the Home Screen.
If you do not wish to change any settings, touch
Cancel
to return to the Home Screen.
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37
CREATE A NEW PROGRAM
From the Home Screen, touch the
Create A New Program
button. This will open a screen where you
can build a program by adding stages and steps and define the temperatures and hold times.
The function of each of the buttons on this screen is described below.
NAME THE PROGRAM
Touch this button to give the program a name.
Type in the required name using the text entry
screen.
Once the name is complete, touch
OK
to return to
the programming screen.
Touch
Cancel
to return to the programming screen
if no changes are required.
When saving the program, if the chosen name has already been used for another program, the message box shown opposite will appear.
Touch OK to return to the Programming screen.
Touch the program name and enter a different
name.
Once the name is complete, touch OK to return to
the programming screen.
Touch
Save
to save the program.
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38
INSERT/DELETE A STAGE
A
stage
is a section of a program that consists of a number of individual steps that can be cycled
(repeated) a defined number of times.
Insert a new stage
To insert a stage in a program, touch
Insert a new
stage
. A stage with one step will be inserted.
Stage 1
Touch this to give the stage a name (optional).
Enter the name using the text entry screen.
1 cycle
Touch this to edit the number of cycles (repeats) for
the stage. Enter the required number of cycles then touch OK.
Temperature
Touch the temperature button to edit the
temperature of the step and add any increment/decrement or gradient features.
Note
: The minimum step temperature that can be programmed during cycling stages is 10°C. If the step temperature is above or below the valid gradient range (PrimeG only) then the gradient function is disabled.
Time
Touch the time button to edit the hold time of the
step and to add any increment/decrement time features.
Ramp rate
Touch the ramp rate setting to adjust the ramp rate.
The range is 0.1ºC/s (minimum) to 3.4ºC/s maximum for heating and 1.0ºC/s for cooling. The value can be entered to 1 decimal place. Ramp rate will affect the heating or cooling rate from the previous step to the current step.
Once set, touch OK to return to the Programming
Screen.
Delete a stage
To
delete
a stage, touch the stage area to highlight
it and then touch
Delete
.
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39
INSERT/DELETE A STEP
Insert a new step
Touch
Insert a new step
to insert the next step in
the current stage.
Edit the new step temperature, hold time and ramp
rate as required.
Delete a step
To delete a step, touch the step area to highlight it
and then touch
Delete
.
INSERT/DELETE A PAUSE
Insert a pause
To insert a pause between stages, touch
Insert a
pause
.
The hold temperature will be that of the previous step. The unit will not continue with the remainder of the program until it is manually un-paused by the user.
Delete a pause
To delete a pause, touch the pause button to
highlight it and then touch
Delete
.
Note
: To insert a pause between two previously programmed stages, it is necessary to first delete the later stages to where the pause is required.
PROGRAM OPTIONS
Touch this to view the program defaults as set up in
the Settings module.
Any of these parameters can be edited by touching the appropriate button and will be saved for the current program only.
Touch
Program
to return to the programming
screen.
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40
User
User names can be entered and linked to the program to help in searching and identifying programs.
To enter a user name, touch the
None selected
button next to User.
User names that have already been created will
then be listed.
To create a new user name, touch the
Create a
new user
button.
Type in the user name and touch OK.
The new user name will be added to the list.
To select a user name, touch the button to highlight
it. Touch it again to de-select it.
To delete a user name from the list, select the user
name and touch
Delete
.
Touch
Done
to return to Program Options.
The user name will now appear in the Program Options screen.
Touch the
Program
button to return the
programming screen.
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41
Keywords
Keywords can be entered and linked to the program to help in searching for related programs.
To enter a keyword, touch the
None selected
button next to Keywords.
Other keywords that have already been created will
then be listed.
To create a new keyword, touch the
Create a new
keyword
button.
Type in the keyword and touch OK.
The new key word will be added to the list.
To select a keyword, touch the button to highlight it.
Touch it again to de-select it. More than one keyword can be selected
To delete a keyword from the list, select the
keyword(s) and touch
Delete
.
Touch
Done
to return to Program Options.
The keyword(s) will now appear on the Program Options screen.
Touch the
Program
button to return the
programming screen.
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42
Passwords
A password can be created to protect the program from being edited and deleted by other users. The password will be requested if a user attempts to edit or delete the program.
To create a password, touch the
None
button next
to Password.
Enter the required password then touch OK. The
password is case-sensitive.
Touch
Cancel
to return to Program Options without
entering a password.
Touch the
Program
button to return to the
Programming screen.
To change or remove the password, from the
Program Options screen, touch the button next to
Password
.
On the Password Editing screen, touch the
None
button and enter the password.
To remove the password, touch
Remove
Password
.
To change the password, touch
Change
Password
and type in the new password, followed
by OK.
Touch
Cancel
to return to the Program Options
screen if no change is required.
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43
SETTINGS
Touch the
Settings
button to view the instrument defaults as set up in the instrument Settings module.
Any of these parameters can be edited by touching the appropriate button and will be saved for the current program only.
Touch
Program
to return to the programming
screen.
VIEW
Touch
View
to see a graphical representation of
the program.
If the program has more stages and steps than are
displayed on the screen, scroll through the program by dragging a finger across the touch screen.
The centre of the screen shows each temperature step and hold time (not to scale). Stages are indicated by a white bar spanning the steps within the stage.
The bottom of the screen shows the name of the program and the expected end time if it was started immediately.
Note
: The program is not saved at this point.
Touch
Start
to start the program.
Touch
Edit
to edit the program.
Touch
Back
to return to the Programming Screen.
Note:
The temperature log will remain blank until the
program has been started.
If a step has a gradient programmed into it, the bar will be shown thicker and indicate the upper and lower temperatures of the gradient range.
If a step has an incremented or decremented temperature, this will be indicated by a white spot next to the temperature.
If a step has an incremented or decremented time, this will be indicated by a white spot next to the time.
Gradient
Inc/dec
temperature
Inc/dec time
Gradient
Inc/dec
temperature
Inc/dec time
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44
DELETE
This is used to delete an entry. First touch the required segment followed by
Delete
.
UP AND DOWN ARROWS
Use these buttons to scroll through a program.
SAVE
Touch this button to save the program.
CANCEL
Touch this button to cancel changes to the program and return to the Home Screen.
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45
RUN A PROGRAM
From the Home Screen, touch one of the shortcuts to run one of the listed programs, or touch
Ru
n a
program
to search and select from other programs and templates saved on the unit. Selected
programs can be run directly or edited before running.
RUNNING A PROGRAM FROM THE SHORTCUT LIST
On the Home Screen, the Run a program area displays shortcuts to four programs. User Preferences in the Settings module can be used to select whether this displays the four most
recently
used
programs or the four most
frequently
used programs.
To run one of the programs in the shortcut list,
touch the button to open the Pre-run Screen.
THE PRE-RUN SCREEN
The Pre-run Screen shows a graphical representation of the program allowing for a rapid check of the temperature parameters.
The centre of the screen shows each temperature step and hold time (not to scale). Stages are indicated by a white bar spanning the steps within the stage.
The bottom of the screen shows the program name, the date and the expected end time if it was started immediately.
Touch
Start
to start the program.
Touch
Edit
to edit the program.
Touch
Back
to return to the previous screen.
Note:
The temperature log will remain blank until the
program has been started.
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46
THE RUN SCREEN
The Run Screen will be displayed after a program has been started. It shows the instrument status and the progress of a run.
Once the program has been started, the timers in the top right hand corner of the screen will indicate how long the program has been running and the total run time. The progress bar at the top of the screen also indicates how far the run has progressed.
Adaptive ramp rate
The first time a program is run, the unit estimates the total run time based on the ramp rates set in the program. After the first cycle has completed the actual time taken to complete the cycle is then used to re­calculate the total run time. Subsequent runs of the same program will therefore have a more accurate estimate of total run time. The estimate also takes into account the ambient room temperature; therefore run times will also depend on ambient temperature.
The status of the block is indicated by the step bars which display the activity of the block (i.e. ramping to temperature), paused or the count down time of the step.
Progress through the program is indicated by the stage bar(s) which show at which cycle of the program the unit is currently at.
Touch
Stop
to stop the program. A prompt will ask
you to confirm the stop.
Touch
Temperature log
to view the block temperatures. The previous two minutes of temperature readings can be viewed by scrolling across the screen using a finger.
Note
: The complete log is written and saved on the unit only if Temperature Log Recording is selected as Always in the Instrument Settings.
Note
: Touch
Program View
to return to the program
status.
Touch
Pause
to pause the program.
If paused, touch
Continue
to un-pause the
program.
Touch
Back
to return to the Home Screen.
Note
: It is possible to access all modules of the software while the unit is running a program. Once the run has finished, Program Finished will be displayed instead of End time.
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47
Viewing a running program
From the Home Screen:
Touch
Current status
.
Touch the area for the instrument.
Touch
View
.
SEARCHING FOR A PROGRAM NOT IN THE SHORTCUT LIST
There are four ways to search for a program if it is not in the shortcut list:
Search from the complete list of saved programs.
Search by keyword.
Search by user.
Search by both keyword and user.
Search from a list
To search for a program saved on the unit, touch
Run a Program
to open the Program Selection
Screen.
Use the + and – buttons to scroll up and down the
list of stored programs.
Touch
Sort By Program Name
to list the programs
in order. The options are:
Program Name (ABC)/(ZYX)
Recently Used (123)/(321)
Most Used (123)/(321)
User (ABC)/(ZYX)
Search the programs by k
eyword
Touch
Search by Keyword
and this will open the
list of keywords stored on the unit.
Select one or more keywords by touching the
buttons to highlight them. Touch again to de-select.
When all keywords have been selected, touch
Done
to return to the Program selection screen.
Only the programs with the selected keyword(s) saved with them will be listed.
To clear the keyword search, touch
Clear Keyword
Search
.
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48
Search the programs by u
ser
Touch
Search by User
and this will open the list of
user names stored on the unit.
Select the required user name by touching the
appropriate button to highlight it. Touch again to de-select.
When the user name has been selected, touch
Done
to return to the Program selection screen.
Only the programs with the selected user name saved with them will be listed.
To clear the user name search, touch
Clear User
Search
.
Search the programs by
keyword and u
ser
Touch
Search by Keyword
and this will open the
list of keywords stored on the unit.
Select one or more keywords by touching the
buttons to highlight them. Touch again to de-select.
When all keywords have been selected, touch
Done
to return to the Program selection screen.
Only the programs with the selected keyword(s) saved with them will be listed.
Touch
Search by User
and this will open the list of user names saved with previously keyword­searched programs.
Select the required user name by touching the
appropriate button to highlight it. Touch again to de-select.
When the user name has been selected, touch
Done
to return to the Program selection screen.
Only the programs with both the selected user name and key word(s) saved with them will be listed.
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49
RUNNING A PROGRAM NOT ON THE SHORTCUT LIST
Once the required program has been identified,
touch it to highlight it.
The Program Selector Screen will show a summary of the program, the instrument settings and a graphical representation of the thermal cycling program.
Touch
Edit
to edit the program if required.
Touch
Back
to return to the Home Screen.
Touch
Start
to proceed to the Pre-run Screen.
From the Pre-run Screen, touch
Start
to run the
program.
See
THE RUN SCREEN
on page 46 for further information on instrument status and progress during a run.
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50
EDITING A PROGRAM
EDITING SAVED PROGRAMS
First select the required program by searching the
Run a program
module.
To edit a selected program, touch the
Edit
button.
Any of the Program Options, Settings or thermal cycling parameters can be edited.
Once all the required changes have been made,
touch
Save
.
Note: this will overwrite the existing program.
To copy a program for editing, please see section
VIEW FILES
.
EDITING TEMPLATES
Editing templates can be a quick method of creating a program by just changing a few key parameters. However it is not possible to save changes to the templates installed on the thermal cycler.
If the edited program is a template, touching
Save
will
open the message box shown opposite.
Touch the
None
button next to New Program
Name and enter a name for the edited program.
Touch
Save As New Program
to save the
changes.
The re-named program will now be listed in the program list.
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51
EDITING PASSWORD PROTECTED PROGRAMS
If the edited program is password protected, touching
Save
will open the message box shown opposite. To
save the changes either:
Touch the
None
button next to Password and
enter the correct password followed by
Save
.
Note: this will overwrite the existing program.
or
Touch the
None
button next to New Program Name and enter a new name for the edited program, followed by
Save as a New Program
.
The re-named program will now be listed as a separate program in the program list.
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52
CURRENT STATUS
The
Current Status
area of the Home Screen provides status information on the Prime and is used to
access the Run Screen.
On the Home Screen, the Current status area shows the versions of software and firmware loaded on the unit.
Touch the
Current status
button to view the
status of the Prime.
The Prime status will be shown.
If a unit is running a program, as shown by the status, the Run Screen can be viewed. Runs can then be paused or stopped as required.
Touch the area to select the unit.
Touch
View
.
Touch
Cancel
to return to the Home Screen.
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53
VIEW FILES
The
View files
module opens the
File Manager
and allows access to all the program and temperature log files. From here it is possible to copy program files to the unit for editing. Files can also be deleted or copied to a USB memory stick for transfer to a PC or storage.
FILE SEARCHING
There are four ways to search for a file:
Search program files or temperature logs from the
complete list of saved files.
Search by keyword.
Search by user.
Search by keyword and user.
To search for a file saved on the unit, touch
View
Files
to open the File Manager screen.
Search from a list
Touch
Programs And Logs
to display either
programs only, temperature logs only or both.
Use the up and down arrow buttons to scroll
through the list of saved files.
Touch
Sort By Program Name
to list the programs
in order. The options are:
Program Name (ABC)/(ZYX)
Recently Used (ABC)/(ZYX)
Most Used (ABC)/(ZYX)
User (ABC)/(ZYX)
Search the
files by keyword
Touch
Search By Keyword
and this will open the
list of keywords stored on the unit.
Select one or more keywords by touching the
buttons to highlight them. Touch again to de-select.
When all keywords have been selected, touch
Done
to return to the File Manager screen.
Only the files with the selected keyword(s) saved with them will be listed.
To clear the keyword search, touch
Clear Keyword
Search
.
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54
Search the
files by u
ser
Touch
Search By User
and this will open the list of
user names stored on the unit.
Select the required user name by touching the
appropriate button to highlight it. Touch again to de-select.
When the user name has been selected, touch
Done
to return to the File Manager screen.
Only the programs with the selected user name saved with them will be listed.
To clear the user name search, touch
Clear User
Search
.
Search the
files by keyword and u
ser
Touch
Search By Keyword
and this will open the
list of keywords stored on the unit.
Select one or more keywords by touching the
buttons to highlight them. Touch again to de-select.
When all keywords have been selected, touch
Done
to return to the Program selection screen.
Only the programs with the selected keyword(s) saved with them will be listed.
Touch
Search By User
and this will open the list of user names saved with previously keyword­searched files.
Select the required user name by touching the
appropriate button to highlight it. Touch again to de-select.
When the user name has been selected, touch
Done
to return to the File Manager screen.
Only the files with both the selected user name and key words saved with them will be listed.
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55
VIEWING A PROGRAM FILE
Touch
Programs And Logs
to display Only
Programs.
Search for the required program file and touch it to
highlight it.
Touch
View
to open the temperature profile (Pre-
run Screen) of the program.
Touch
Start
to start the program.
Touch
Edit
to edit the program.
Touch
Back
to return to the previous screen.
Note
: The temperature log will remain blank until the
program has been started.
VIEWING A TEMPERATURE LOG FILE
It is not possible to view a temperature log file on the Prime. It must first be copied to a USB memory stick then viewed on a PC using the Techne Workbench software, which can be downloaded free of charge from www.techne.com
See the directions given in the next section for
copying files to a USB memory stick.
Copy the files to a PC and open the Workbench
software.
Use
Settings
to select the path for the saved files
or click on
Open
to browse for the saved files.
Select the required temperature log and click
Open
.
Note
: Since the .temperaturelogdata file is a text file, it can also be opened in applications such as Excel for further analysis.
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56
When opening a temperature log file, the Temperature Log Monitor screen will be displayed. To view the complete temperature log, drag the mouse across the screen to scroll through the data points.
The view will show a graphical representation of the actual temperature changes, second by second, throughout thermal cycling program.
Clicking on the
Program View
button of this screen will display the program that generated the temperature log.
For further information on using the Techne Workbench software, see the separate user manual which can be downloaded from www.techne.com
COPYING FILES
Files can either be copied onto the unit for editing, or to and from a USB memory stick.
To copy files to the unit:
Select one or more files for copying by touching the
appropriate file button.
Note
: Multiple file selection is optional and can be set up in the User Preferences section of the Settings Module.
or
Touch
Select All
to select all files.
Touch
Copy
.
A message box will confirm that the files have been copied.
The copied file(s) will now be highlighted and listed as Copy of… and can be edited as required.
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57
To copy files to a USB memory stick:
Insert a USB memory stick into the port on the front
of the unit.
A message screen will appear confirming that the USB memory stick has been inserted.
Touch
Cancel
to return to the File Manager
Screen.
Select one or more files for copying by touching the
appropriate file button.
or
Touch
Select All
to select all files.
Touch
Copy
.
A message screen will appear giving the option of saving the files to the unit or to the USB memory stick.
Touch the upper button to save the file(s) to the
USB memory stick.
Touch
Cancel
to return to the File Manager screen.
The file(s) will be saved on the USB memory stick in a folder called FileSystem as a .cyclerprogram file.
To copy files from a USB memory stick:
Insert a USB memory stick into the port on the front
of the unit.
A message screen will appear confirming that the USB memory stick has been inserted.
Touch
Yes
to view the programs on the USB
memory stick in the File Manager.
A USB button will be visible in the top right corner indicating that the files are being read from the USB memory stick.
Select one or more files for copying by touching the
appropriate file button.
or
Touch
Select All
to select all files.
Touch
Copy
.
A message screen will appear giving the option of saving the files to the unit or to the USB memory stick.
Touch the upper button to save the file(s) to the
Prime unit.
Touch
Cancel
to return to the File Manager screen.
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58
DELETING FILES
Unused or old files can be permanently deleted from the unit or a USB memory stick.
Select one or more files for deleting by touching the
appropriate file button.
Note
: Multiple file selection is optional and can be set up in the User Preferences section of the Settings Module.
or
Touch
Select All
to select all files.
Touch
Delete
. A message box will appear asking
for confirmation of delete.
Touch
Yes
to confirm the delete.
Touch
Cancel
to abort the delete.
If the delete is confirmed, a message box will confirm that the files have been deleted.
Touch OK.
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59
UPDATING THE PRIME SOFTWARE
The Prime software can be updated by downloading the latest version from the Techne website
www.techne.com. You will need a USB memory stick on which to save the update.
Note that the update tool requires at least Windows XP SP2 and the Microsoft .Net Framework 3.5 SP1, which, if needed, can be downloaded from:
http://www.microsoft.com/downloads/details.aspx?familyid=AB99342F-5D1A-413D-8319­81DA479AB0D7&displaylang=en
From the website, click on
Support
followed by
Software Downloads
.
Select the
Prime Update
and choose the
option to run the file.
Insert a USB memory stick into the PC and
click to select.
Click on
Prepare the USB memory stick
.
This will copy the update to the USB memory stick.
Turn the Prime off and insert the USB stick into
the front USB port.
Turn the Prime on and follow the instructions
on the screen.
The update will take several minutes. When finished, the home screen will be displayed.
Remove the USB memory stick from the
Prime.
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UPGRADING FROM P
RIME
TO P
RIME
G
The standard Prime thermal cycler can be easily and quickly upgraded to the gradient PrimeG version by purchasing the PrimeX/USB upgrade. The upgrade consists of a license certificate printed with a unique upgrade code which allows gradient functionality to be installed on any non-gradient Techne Prime thermal cycler.
Purchase of this license certificate authorises the owner of any non-gradient Techne Prime thermal cycler to lawfully utilise the gradient capability of that thermal cycler in all European and American geographies. The license is machine-specific and non-transferable.
Gradient upgrade procedure:
Turn on the Prime unit.
On the home screen, touch
Settings
and then
More Settings
, followed by
Add Gradient
Feature
.
Go to the address www.techne.com. Click on
Support
then
Technical Support
. Under
Further Information
click on
Thermal Cycler
Gradient Upgrade
.
Insert the serial number from the Prime into the
website
twice
as instructed.
Insert the upgrade code printed on the certificate
into the website.
Click
Show confirmation number
.
Insert the upgrade code and confirmation
number into the Prime and touch
Validate
.
The Prime is now upgraded.
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61
REMOVING AND EXCHANGING BLOCKS
The thermal cycling block can be removed and exchanged for one of a different format or for detailed cleaning.
1. Before removing the block, disconnect from the power
supply and allow it to cool to below 50°C.
2. Slide the quick release handles in the direction of the arrows
indicated and lift the block assembly out of the unit.
3. Never lift or carry the block by one quick release handle,
always use both handles or support the block from underneath.
4. When re-fitting the block, or fitting a new one, slide the quick
release handles in the opposite direction to lock the assembly into the unit.
5. Open or closed padlock symbols are printed on the quick
release handles for easy reference.
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FAQs
Q1
How do I adjust the pressure of the heated lid for my tubes or plates?
A1
For thermal cyclers with lid adjustment knobs, rotate the knob anticlockwise until there is no pressure on the consumable, then close the lid and latch it. To obtain the correct pressure gently rotate the knob clockwise until it is possible to just feel the pressure being applied. Finally, rotate the knob a further quarter of a turn; the lid is now at the correct pressure for use with the consumable. Ensure that all consumables used in the block are of the same height and are spread evenly across the block. Insert empty “dummy” tubes if necessary to spread the pressure of the heated lid evenly.
Q2
What is the '”Pause” function at the start of the program used for?
A2
Some users prefer to preheat the heated lid before placing the samples into the unit. The pause feature is used to pause the unit after the 2 minute heated lid preheat step.
Q3
What is “Sample Cooling”?
A3
Sample cooling can be applied during the lid pre-heat phase. This maintains the block at 4ºC while the lid is heating to temperature and avoids any increase in sample temperature due to the heated lid before the thermal cycling program begins.
Q4
What is "Hot start"?
A4
The Hot Start step is used to pause the machine at a specific temperature, typically around 70°C, after the initial template denaturation. The reason is to allow the manual addition of unmodified
Taq
DNA polymerase which may lose activity if added during the
initial denaturation step. Heat-activated
Taq
or Hot Start enzymes do not require this step.
Q5
What is the incremented time and temperature function for?
A5
Incremented/decremented time and temperature are used to increase or decrease either the time or temperature incrementally over the number of cycles in a stage. Incrementation of extension time is used with 'Long PCR' which is when large template fragments are to be amplified (e.g. 27kb lambda DNA, 40kb genomic DNA). Decremented temperature is used for protocols such as 'Touchdown PCR' where the first cycle starts with a high annealing temperature and over the number of cycles in the stage there is a gradual decrease in the temperature. This ensures that only the specific product is amplified.
Q6
What is a gradient thermal cycler?
A6
Gradient blocks enable a particular temperature step in a protocol to be programmed so that the temperature varies across the block. By specifying a temperature and the gradient to be applied, each column will achieve a different temperature. The set temperature is in the middle of the block, the lowest on the left and the highest on the right. The gradient is the total difference across the block, for example if the set temperature was 60°C and the gradient 10°C then the temperatures would range from approximately 55°C to 65°C from left to right.
Q7
Why is a temperature gradient required?
A7
The annealing temperature of the specific primers used in DNA amplification often requires optimisation. Instead of running multiple experiments with different annealing temperatures the gradient thermal cycler (PrimeG) allows the testing of up to 12 different annealing temperatures simultaneously.
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63
TECHNICAL SUPPORT AND SERVICING
If you require further technical or application assistance please contact Techne at:
E-mail: technehelp@bibby-scientific.com. Phone: +44 (0)1785 810433 Fax: +44 (0)1785 810405
For servicing information please contact:
Service Department Bibby Scientific Ltd. Beacon Road Stone Staffordshire ST15 0SA E-mail: service@bibby-scientific.com.
Phone: +44 (0)1785 810475 Fax: +44 (0)1785 810405
In the USA please contact service@techneusa.com or call +1 609 589 2560.
We are continually striving to improve our products and software. If you have any comments and suggestions on how we can do things better please send them to us at: techne@bibby-scientific.com.
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ADDITIONAL INFORMATION
USER MAINTENANCE
Before cleaning your unit, disconnect it from the power supply. The outer case of the Prime may be cleaned with a cloth dipped in water or ethanol (hexane or 50% methanol can also be used). No part of the case or cover should be immersed in the solvents. Do not use aggressive solvents such as acetone or abrasive cleaners. The block may be wiped with water, ethanol or propan-2-ol and may be decontaminated by wiping with 2% Neutracon or 1% bleach solution.
Before using any cleaning or decontamination method except those recommended here, the responsible person should check with Techne that the proposed method will not damage the equipment.
FAULT FINDING
Note that this equipment should only be dismantled by properly trained personnel. Removing the outer cover exposes potentially lethal mains voltages. There are no user serviceable parts within this equipment.
Should you have any problems with your Prime which cannot be easily remedied, you should contact your supplier and return the unit if necessary. Please include details of the fault observed and remember to return the unit in its original packing. Bibby Scientific Ltd. accepts no responsibility for damage to units which are not properly packed for shipping: if in doubt, contact your supplier, giving the full serial number of the unit.
FUSES
If the display on the front panel is not lit, one of the two fuses may have blown. Check that there is no external cause, such as a faulty plug or lead. Check both fuses and replace the faulty fuse with a new one of the correct value (fuse values are given on the label next to the power inlet). Note that fuses should only be replaced by a qualified electrician.
The holder for the two fuses is built into the mains input socket. First remove the power cable and then gently prise the fuse drawer open with a flat-bladed screwdriver or similar tool. Each fuse can be removed by using the screwdriver as a lever.
Exchange the faulty fuse in the fuse holder for a working fuse of the correct value. Finally, replace the fuse drawer in the fuse compartment and push the drawer shut. Fuses which blow repeatedly indicate a serious fault and you should return the unit to your supplier for repair.
INSULATION TESTING
This equipment is fitted with RFI suppression circuitry. Any check of the electrical insulation by means of high voltage dielectric testing (for example as in BS EN 61010-1) must be carried out using only a DC voltage.
This unit contains semiconductor components which may be damaged by electric field effects.
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ACCESSORIES
The following accessories can be obtained from Bibby Scientific Ltd. or your Techne distributor:
Product code
Description
Prime/02/B Prime block, 96 x 0.2ml Prime/05/B Prime block, 60 x 0.5ml Prime/384/B Prime block, 384 well plates PrimeX/USB Prime base gradient upgrade
REPLACEMENT PARTS
The following replacement parts can be obtained from Bibby Scientific Ltd. or your Techne distributor:
Product code
Description
HH179(S) UK 230V mains lead with plug HH180(S) European mains lead with plug FCABLEUS US mains lead with plug
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