Techne Hybrigene User Manual
Hybrigene
Hybridisation oven
OPERATOR’S MANUAL
Issue 7
07/2014
2
CONTENTS
INTRODUCTION ........................................................................................................ 5
BEFORE USE............................................................................................................. 6
Unpacking .................................................................................................................................................6
SAFETY AND INSTALLATION .................................................................................. 7
Warning ....................................................................................................................................................7
Operator Safety ........................................................................................................................................7
Installation ................................................................................................................................................7
Environmental Conditions ........................................................................................................................8
Guarantee .................................................................................................................................................8
SÉCURITÉ ET CONSIGNES D’INSTALLATION ....................................................... 9
Avertissement ...........................................................................................................................................9
Sécurité de l’opérateur .............................................................................................................................9
Installation ................................................................................................................................................9
Conditions environnementales .............................................................................................................. 10
Garantie ................................................................................................................................................. 10
SICHERHEITS- UND INSTALLATIONSINFORMATIONEN .................................... 11
Achtung ................................................................................................................................................. 11
Sicherheit des Bedienpersonals ............................................................................................................ 11
Inbetriebnahme...................................................................................................................................... 11
Umweltbedingungen .............................................................................................................................. 12
Garantie ................................................................................................................................................. 12
INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONE ................................. 13
Avvertenza ............................................................................................................................................. 13
Sicurezza dell'operatore ........................................................................................................................ 13
Installazione ........................................................................................................................................... 13
Condizioni ambientali ............................................................................................................................ 14
Garanzia ................................................................................................................................................ 14
INFORMACIÓN DE SEGURIDAD E INSTALACIÓN ............................................... 15
Advertencia ............................................................................................................................................ 15
Seguridad del operario .......................................................................................................................... 15
Instalación ............................................................................................................................................. 15
Condiciones ambientales ...................................................................................................................... 16
Garantía ................................................................................................................................................. 16
CONTACT INFORMATION ...................................................................................... 17
SPECIFICATIONS .................................................................................................... 18
INSTALLATION ........................................................................................................ 19
Fitting stacking plates and wall mounting plates ................................................................................... 19
OPERATION ............................................................................................................ 21
Switching On ......................................................................................................................................... 21
The front panel controls ......................................................................................................................... 21
Setting the temperature ......................................................................................................................... 22
Setting the speed .................................................................................................................................. 22
Hybridisation tubes ................................................................................................................................ 23
Installing the hybridisation tubes ........................................................................................................... 25
Basic protocol for Southern Hybridisations ........................................................................................... 25
Warning ................................................................................................................................................. 25
User maintenance ................................................................................................................................. 26
ADDITIONAL INFORMATION.................................................................................. 27
Fault Finding .......................................................................................................................................... 27
Fuses ..................................................................................................................................................... 27
Safety over-temperature cut-out............................................................................................................ 27
Support contacts.................................................................................................................................... 27
SPARES AND ACCESSORIES ............................................................................... 28
3
DECLARATION OF CONFORMITY ......................................................................... 29
4
INTRODUCTION
Model number
Description
Voltage
Please read all the information in this manual before using the unit.
The Techne Hybrigene hybridisation oven is ideal for blotting techniques in which RNA, DNA or
protein are immobilised onto nylon or nitrocellulose filters. Several filters can be hybridised
simultaneously in a single tube while the rotating design ensures volumes as low as 5ml can be used
and recovered.
The Hybrigene has unique “slot-in” tubes with adjustable tube rotation speeds of 0 to 20 rpm;
controllable to suit your application. The tubes and other accessories can be accommodated together
for multiple applications and the unit can hold up to 16 mini or 4 large glass tubes. The temperature
can be set from 10°C above ambient to 80°C and offers excellent temperature accuracy and
uniformity.
For safety, the unit has a 10mm thick Perspex door to shield the immediate environment from
radiation. In addition, protective casing and non-drip tube design minimises risk to the user. Included
is a removable drip tray for easy cleaning should spillages occur.
The Hybrigene has adjustable feet for levelling on uneven surfaces. It is also possible to stack up to
three ovens, saving on valuable laboratory space.
The operating instructions in this Operator’s Manual cover the following models of the Techne
Hybrigene:
FHB4DD Hybrigene hybridisation incubator 230V, 50/60Hz
FHB4DDT
FHB4DP Hybrigene hybridisation incubator 120V, 50/60Hz
FHB4DPT
Hybrigene hybridisation incubator, (including 4 large
tubes FHB16)
Hybrigene hybridisation incubator, (including 4 large
tubes FHB16)
230V, 50/60Hz
120V, 50/60Hz
5
BEFORE USE
Before using the Hybrigene please make sure you have read this manual carefully. If there is any
doubt relating to the proper use of this equipment, the staff at Bibby Scientific Ltd. or your supplier
will be happy to assist you.
UNPACKING
When unpacking the unit please ensure that the following have been removed from the packaging:
• Hybrigene oven including drip tray
• Mains cables (UK and EU or US)
• Tube of silicon grease
• Hybridisation tubes x 4 (if included)
• Operator’s manual
• Guarantee card
• Decontamination certificate
The user is advised to keep the original packaging in case the instrument ever needs to be returned
for service or repair. Bibby Scientific Ltd. accepts no responsibility for damage incurred unless the
unit is correctly packed and transported in its original packaging.
6
SAFETY AND INSTALLATION
Connection
s 230V
120V
Live Brown
Black
Neutral
Blue White
Earth
Green/yellow
Green
Please read all the information in this manual before using the Hybrigene.
WARNING
HIGH TEMPERATURES ARE DANGEROUS: they can cause serious burns to operators and ignite
combustible material.
Techne have taken great care in the design of these units to protect operators from hazards, but
operators should pay attention to the following points:
• USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS
• DO NOT use combustible substances near hot objects
• DO NOT operate the instrument in the vicinity of inflammable liquids or gases.
• DO NOT place any liquid directly into the instrument.
OPERATOR SAFETY
All operators of Techne equipment must have available the relevant literature needed to ensure their
safety. It is important that only suitably trained personnel operate this equipment, in accordance with
the instructions contained in this manual and with general safety standards and procedures. If the
equipment is used in a manner not specified by Bibby Scientific Ltd. the protection provided by the
equipment to the operator may be impaired.
All Techne instruments are designed to conform to international safety requirements and are fitted
with an over-temperature cut-out.
If a safety problem should be encountered, switch off the unit at the mains socket and remove the
plug from the electricity supply.
INSTALLATION
The instrument should be carried using both hands. Never move or carry the instrument when in use
or connected to the mains electricity supply.
1. All Techne instruments are supplied with a power cable; this may be integral or plug-in.
2. Before connecting the instrument to the mains electricity supply, check the voltage
against the rating plate (located on the back of the unit). Please note that the unit
must be earthed to ensure proper electrical safety. Connect the mains cable to a
suitable plug according to the table below.
UK ONLY: The fused plug supplied with the mains cable is fitted with a 5 amp fuse to protect the
instrument and the operator.
Note that units marked 230V on the rating plate work at 220V; units marked 120V work at 110V. In
both cases, however, the heating rate will degrade by approximately 8%.
3. Place the unit on a suitable flat bench or in a fume cupboard if required, ensuring that the air inlet
vents on the underside are free from obstruction.
4. Plug the mains cable into the socket on the back of the instrument.
5. Switch on the instrument. Symbols on or near the power switch of the unit have the following
meanings:
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I Mains Switch On
O Mains Switch Off
Replacement cable
Should the mains lead need replacement, a cable of 1mm2 of harmonized code H05VV-F connected
to an IEC320 plug should be used. IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN.
ENVIRONMENTAL CONDITIONS
The Hybrigene is designed operate under the following conditions:
• Indoor use
• Ambient temperature range +5ºC to +40ºC
• Altitude to 2000m
• Relative humidity up to 80%
• Mains supply fluctuations not exceeding 10%
• Over voltage category II IEC 60364-4-443
• Pollution degree 2
• Use with a minimum distance all-around of 200mm from walls or other items
Note: The control specifications are quoted at an ambient temperature of 20ºC. The specification
may deteriorate outside an ambient temperature of between 18ºC and 30ºC.
The instrument has been tested for radio frequency interference and is certified under EN61326.
GUARANTEE
The instrument is guaranteed against any defect in material or workmanship for the period specified
on the enclosed guarantee card. This period is effective from the date of purchase; within this period
all defective parts will be replaced free of charge provided that the defect is not the result of an
accident, misuse or negligence. Servicing under this guarantee should be obtained from the supplier
of the instrument.
This manual has been prepared for the convenience of Techne’s customers and nothing in this
manual shall be taken as a warranty, condition or representation concerning the description,
merchantability, fitness for purpose or otherwise of the unit or components.
Notwithstanding the description and specification(s) of the instruments contained in the operator’s
manual, Techne reserves the right to make such changes as it sees fit to the instruments or to any of
the components.
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SÉCURITÉ ET CONSIGNES D’INSTALLATION
Connexion
s 230 V 120 V
Phase
Marron
Noir
Neutre
Bleu Blanc
Terre
Vert/jaune
Vert
Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le Hybrigene.
AVERTISSEMENT
Les TEMPÉRATURES ÉLEVÉES SONT DANGEREUSES car elles peuvent provoquer de graves
brûlures chez l’opérateur et enflammer les matériaux combustibles.
Techne a apporté un soin tout particulier à la conception de ces appareils de façon à assurer une
protection maximale des opérateurs, mais il est recommandé aux utilisateurs de porter une attention
spéciale aux points suivants :
• PROCEDER AVEC PRUDENCE ET PORTER DES GANTS POUR SE
PROTEGER LES MAINS
• NE PAS utiliser de matériaux combustibles auprès d’objets chauds.
• NE PAS utiliser l’appareil à proximité de liquides ou de gaz inflammables
• NE PAS verser de liquides directement dans l’appareil.
SECURITE DE L’OPÉRATEUR
Tous les utilisateurs de produits Techne doivent avoir pris connaissance des consignes et instructions
nécessaires à la garantie de leur sécurité. Important: cet appareil doit impérativement être manipulé
par un personnel qualifié et être utilisé selon les instructions données dans ce document, en accord
avec les normes et procédures de sécurité générales. Dans le cas où cet appareil ne serait pas utilisé
selon les consignes précisées par Bibby Scientific Ltd., la protection pour l’utilisateur ne serait alors
plus garantie.
Tous les appareils Techne sont conçus pour répondre aux normes de sécurité internationales et sont
dotés d’un coupe-circuit en cas de surchauffe.
En cas de problème de sécurité, couper l’alimentation électrique au niveau de la prise murale et
enlevez la prise connectée à l’appareil.
INSTALLATION
Porter l'appareil à deux mains. Ne jamais déplacer ou transporter l’appareil lorsqu’il est en
fonctionnement ou branché à l’alimentation électrique.
1. Tous les appareils Techne sont livrés avec un câble d'alimentation, qui peut être intégré à l'appareil
ou à raccorder.
2. Avant de raccorder l’appareil à l’alimentation électrique sur secteur, vérifier la tension
requise indiquée sur la plaque d’identification (située au dos de l’appareil). Il est
important que l’appareil soit relié à la terre pour assurer la protection électrique
requise. Brancher le câble secteur sur une prise appropriée, voir tableau ci-après.
ROYAUME-UNI SEULEMENT: La prise avec fusible intégré fournie avec le câble secteur est
munie d’un fusible 5 A destiné à protéger l’appareil et l’utilisateur.
Remarque : les appareils dont la plaque indique 230 V peuvent fonctionner sur 220 V, et ceux dont
la plaque indique 120 V peuvent fonctionner sur 110 V. Dans les deux cas cependant, la capacité
de chauffage diminuera d’environ 8 %.
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