Techne Culture Vessel User Manual

M i c r o C a r r i e r S t i r r e r O P E R A T O R ’ S M A N U A L
Description
A permanent magnet is sealed into the flexibly mounted stirrer. The position of the stirrer bulb is controlled by another magnet in the MCS platform mounted on a pulley wheel. As the pulley wheel turns the magnet orbits under the culture vessel causing the stirrer bulb to trace a gentle path around the trough in the bottom of the vessel.
The electronic speed control ensures the stirring speed is consistent and repeatable at all settings.
The soft start facility causes the stirrer to increase (or decrease) speed gradually over a period of 20 seconds until the set speed is reached (or stirring stops).
The interval mode allows stirring to be programmed for “on” periods from 6 seconds to 5 minutes and “off” periods from 2 minutes to 2 hours.
Standard culture vessels are calibrated in volume and have two necks and a central filling port. There is also a 5 litre vessel which has five necks and a central filling port. All culture vessel parts are suitable for repeated autoclaving at 121°C and 15psi for 30 minutes. All internal components are biologically inert.
Both the culture vessel and the stirring rod are siliconised before they leave the factory.
The MCS platforms are designed for incubator environments with a maximum temperature of 40°C and 95% relative humidity, non condensing.
P A G E 15
M i c r o C a r r i e r S t i r r e r O P E R A T O R ’ S M A N U A L
Specification
Speed Range 0 to 80 rev/min Speed Setting Accuracy Better than 3 rev/min SOFTSTART Speed Control 20 sec acceleration
20 sec deceleration
Interval stirring
variable on-time 6 sec to 5 min variable off-time 2 min to 2 hrs
Drive Non slip magnetic drive
between rotating magnets in the base and embedded magnets in each stirring rod.
Heat Input to Culture Vessels Negligible. Continuous running
produces no measurable increase
in temperature.
Limiting Operating Conditions 40°C and
95% relative humidity
non-condensing.
Glassware Borosilicate glass, siliconised.
P A G E 16
Electrical Supply: Prior to ser No /4 (can be changed at the transformer)
Voltage Cycles Power (nominal) Fuse
220/250V 50/60Hz 2 W 100 mA
110/120V 50/60Hz 2 W 100 mA
Serial No /4 on (with voltage selector switch)
Voltage Cycles Power (nominal) Fuse
230/120V 50/60Hz 2 W 100 mA
The units, vessels and stirring action are covered by various Patents including: UK 2054397; EUR 0053869; US 4382685; US 3854704.
M i c r o C a r r i e r S t i r r e r O P E R A T O R ’ S M A N U A L
Dimensions
Stirrer Units MCS-101L MCS-102L MCS-104S MCS-104L MCS-104XL Width mm 395 140 250 395 530 Depth mm 495 400 365 495 595 Height mm 90 90 90 90 90 Net Weight 6.5Kg 3.0Kg 4.1Kg 6.1Kg 11.4Kg (excl vessel) Vessels per system 1 2 4 4 4 Max Vessel Size 5 litre 1 litre 500 ml 5 litre 5 litre
Culture Vessels 125 ml 250 ml 500 ml 1 litre 3 litre 5 litre Gross Volume 250 ml 500 ml 1 litre 2 litre 6 litre 10 litre
Nom Working Vol 125 ml 250 ml 500 ml 1 litre 3 litre 5 litre Working Vol 50 to 100 to 200 to 500 to 1.5 to 2 to Range 175 ml 350 ml 700 ml 1500 ml 3.5 litre 7 litre Height 145 mm 170 mm 205 mm 263 mm 284 mm 365 mm Diameter 65 mm 80 mm 100 mm 140 mm 215 mm 240 mm Top Thread Size 42 mm 42 mm 42 mm 42 mm 60 mm 60 mm Side Thread Size 20 mm 20 mm 30 mm 42 mm 42 mm 24 mm Side port size 14 mm 14 mm 23 mm 33 mm 33 mm 18 mm No of side necks 2 2 2 2 2 5
Sterilisation of Glassware
In the interests of public health and the safety of our employees we cannot accept any returned glassware without our previous authorisation.
We require a certificate of sterilisation from the user before giving such authorisation.
A suitable form of certificate is enclosed with the glassware when it is despatched from Techne.
P A G E 17
M i c r o C a r r i e r S t i r r e r O P E R A T O R ’ S M A N U A L
All Units
Replace the cover and fit the appropriate location bosses.
The location boss for 1 litre vessels is supplied with the flask.
500 ml, 250 ml and 125ml flasks use the same location boss, as supplied with the platform.
Siliconising Glassware
All glassware is siliconised before being dispatched. It will eventually require resiliconising in order to maintain its water repellant qualities.
CAUTION: THE WORKROOM MUST BE WELL VENTILATED.
Clean the glassware thoroughly and ensure it is free from grease.
Wash out and immerse the glassware in a water repelling treatment such as Merck Ltd, “Repelcote VS”.
Make sure the glassware is fully wetted by the solution.
Allow the solvent to evaporate. There will normally be sufficient absorbed by water on the glassware to hydrolyse “Repelcote VS” to a highly water repellent silicone film.
P A G E 20
M i c r o C a r r i e r S t i r r e r O P E R A T O R ’ S M A N U A L
Assembling the Culture Vessel
Push the grooved end of the stirrer rod into the flexible suspension joint (item 40).
Fit the washer (item 38) over the rod and push the suspension joint onto the flask cap adaptor (item 39).
Complete the vessel assembly as shown.
In culture vessels of more than 500 ml the length of the stirrer rod may need to be adjusted to ensure that the stirrer bulb clears the bottom of the trough.
This is done using the screw and lock-nut in the flask cap adaptor. Note: This screw and nut are of special material to maintain hygienic conditions.
See the following pages for lists of parts.
P A G E 21
M i c r o C a r r i e r S t i r r e r O P E R A T O R ’ S M A N U A L
Culture Vessel Parts
ITEM PART DESCRIPTION QTY NOTES 125 ml VESSEL, F7988 35 F7987 FLASK 1
36 6007989 STIRRER ROD 1 37 6007629 SEAL 2 38 6007625 SEALING WASHER 1 39 6007627 FLASK CAP ADAPTOR 1 40 6007634 FLEXIBLE JOINT 1 41 6007630 CAP 20mm 2 42 6007632 CAP 42mm 1
250 ml VESSEL, F7689 35 F7690 FLASK 1
36 6007635 STIRRER ROD 1 37 6007629 SEAL 2 38 6007625 SEALING WASHER 1 39 6007627 FLASK CAP ADAPTOR 1 40 6007634 FLEXIBLE JOINT 1 41 6007630 CAP 20mm 2 42 6007632 CAP 42mm 1
500 ml VESSEL, F7607 35 F7609 FLASK 1
36 6007619 STIRRER ROD 1 37 6007623 SEAL 2 38 6007625 SEALING WASHER 1 39 6007627 FLASK CAP ADAPTOR 1 40 6007634 FLEXIBLE JOINT 1 41 6007631 CAP 30mm 2 42 6007632 CAP 42mm 1
P A G E 22
M i c r o C a r r i e r S t i r r e r O P E R A T O R ’ S M A N U A L
ITEM PART DESCRIPTION QTY NOTES 1 LITRE VESSEL, F7608 35 F7610 FLASK 1
36 6007620 STIRRER ROD 1 37 6007624 SEAL 2 38 6007626 SEALING WASHER 1 39 6105066 FLASK CAP ADAPTOR 1 40 6007634 FLEXIBLE JOINT 1 41 6007632 CAP 42mm 2 42 6007633 CAP 60mm 1 44 6400049 NUT S/S 1 45 6400048 SCREW S/S 1 Flasks 255mm High 45 6008729 SCREW S/S 1 Flasks 272mm High
3 LITRE VESSEL, FA298 35 FA299 FLASK 1 (276mm high)
36 6007621 STIRRER ROD 1 For old flask F7615 36 6100290 STIRRER ROD 1 For FA299 37 6007624 SEAL 2 38 6007626 SEALING WASHER 1 39 6105066 FLASK CAP ADAPTOR 1 40 6007634 FLEXIBLE JOINT 1 41 6007632 CAP 42mm 2 42 6007633 CAP 60mm 1 44 6400049 NUT S/S 1 45 6400048 SCREW S/S 1 FA299 flasks and
F7615 flasks 331mm high
45 6008729 SCREW S/S 1 F7615 flasks
351mm high
P A G E 23
M i c r o C a r r i e r S t i r r e r O P E R A T O R ’ S M A N U A L
ITEM PART DESCRIPTION QTY NOTES 5 LITRE VESSEL (5 NECK), FA296 35 FA297 FLASK 1 (357mm high)
36 6007622 STIRRER ROD 1 For older flask F7884
437mm high 36 6100289 STIRRER ROD 1 For FA297 37 6007958 SEAL SMALL 5 37 6007959 SEAL LARGE 5 38 6007626 SEALING WASHER 1 39 6105066 FLASK CAP ADAPTOR 1 40 6007634 FLEXIBLE JOINT 1 41 6007957 CAP 12mm 5 42 6007633 CAP 60mm 1 43 6007960 SEAL WASHER SMALL 5 43 6007961 SEAL WASHER LARGE 5 44 6400049 NUT S/S 1 45 6400048 SCREW S/S 1
5 LITRE VESSEL (2 NECK), FA709 35 FA710 FLASK 1
36 6100289 STIRRER ROD 1 37 6007624 SEAL 2 38 6007626 SEALING WASHER 1 39 6105066 FLASK CAP ADAPTOR 1 40 6007634 FLEXIBLE JOINT 1 41 6007632 CAP 42mm 1 42 6007633 CAP 60mm 1 44 6400049 STAINLESS NUT 1 45 6400048 STAINLESS SCREW 1
P A G E 24
Loading...