Sri 210D User Manual

HPLC

Model 210D

1. Overview

The Model 210D front panel houses the UV detector lamp switch, Rheodyne injection valve, prime/purge
valve, pressure gauge, and the storage
compartment. In the storage compartment
are the LED display for the column
temperature, and the vacuum degas pressure

UV detector
lamp switch

Rheodyne 7725i injection valve

Prime/purge

valve

gauge (shown above). An accessories kit is
included with your 210D. In the kit are two

Column
compartment
thumbscrews

PEEK finger tight nuts (Alltech part # 32233)
for column connection, a spare 1/16”
stainless steel fitting (Valco part ZN1) and
ferrule (Valco part ZF1S6), a flange fitting

Storage
compartment

Pressure gauge

(Upchurch part 203X) and ferrule
(Upchurch part 240X) for the solvent recycle valve, a 20mL priming syringe (Sigma Aldrich part Z248037),
and a 100µL glass injection syringe (SGE part 005300).

On the right hand side of the 210D is the column compartment, which gives you access to the column heater,
pump head(s), solvent recycle valve, and the detector housing. Open the column compartment by loosening
the two captive thumbscrews and lifting off the cover. Use the picture below to familiarize yourself with the
interior of the column compartment.

Column heater

Rheodyne 7725i
injection valve
with a 20µL
sample loop

Prime/purge
valve

Pump outlet line

UV and

Conductivity

detectors

Pressure
gauge

Pulse damper

Vacuum

de-gas

outlet

Vertical

slide

Pump inlet linesPump heads

Solvent recycle valve

Vacuum de-gas coil

HPLC
Model 210D

2. Installing a Column

Slide the column heater up.

Install the column of your choice using the included PEEK finger tight nuts. Slide the
nuts onto the PEEK tubing, letting about 1mm of PEEK tubing protrude.

Secure one nut to the column.

Slide the column through the column heater so you can reach the other end,
and attach the second nut. Roughly center the column in the column heater.

Slide the column heater up
on its aluminum slider.

Replace the black column heater end caps, which have notches in them for the PEEK tubing. Pack in any
loose insulation. When you have replaced both end caps as shown below, slide the column heater back down
into the operating position.

3. Solvent Set-up

The pump heads each have an 1/8” Teflon solvent inlet line. Place
the ends of these lines in your solvent bottle. The solvent recycle
valve has two outlet lines. The 1/16” clear line is recycled solvent.
Put it into the solvent bottle, or into its own container if you wish
to keep it separate from your fresh solvent. Place the end of the
1/16” green line into a waste bottle. A simplified way to remember:
all the clear lines go to the solvent bottle. In default mode, the
solvent recycling valve sends the effluent out the clear tube; when
activated, it directs the effluent out the green waste tube.

1/4” tygon tubing

piston flush inlet line

HPLC

Model 210D

Solvent recycle valve

Blue = in

from column

Clear =

recycled

solvent out

Green =

to waste

The 1/4” Tygon tubing attached to the pump
head(s) is the piston flush line. Piston flushing
helps keep the high pressure seal(s) clean,
minimizing wear and tear from crystallized and
abrasive buffer deposits that leak through the
seal, and would otherwise dry on the piston.
There are inlet and outlet lines for piston
flushing. Place the ends of both into a bottle
containing at least 50% organic solvent (usually
diluted with deionized water). Binary gradient
pump head piston flush lines are daisy chained,
and share the same in and out lines.

Piston flush

outlet line

210D pump, side view:

It is possible for solvent to leak past
the high pressure seal with the back
and forth thrusting of the piston.

Piston

Piston flush IN

High pressure seal

Low pressure seal

Piston flush OUT

Pump outlet

Pump inlet

HPLC
Model 210D

4. Vacuum De-gas

While not all HPLC users degas their solvent, it is a good idea to
do so. The 210D is equipped with an air pump for vacuum
degassing. A length of tygon tubing is provided to connect the
pump to the degas fitting on the left-hand side of the HPLC. The
tubing is equipped with a nut for easy connection to the degas fitting.
With the 210D power ON, connect the pump tubing to the degas
fitting, then plug the pump into a wall outlet. Open the storage
comparment to see the vacuum degas gauge, which should read
7psi with the pump ON and solvent lines attached.

The vacuum degas gauge should

read 7psi during operation.

Simply screw this nut onto the degas fitting
to connect the vacuum pump to the 210D.

Power switch

Access
terminals

Serial or USB
cable to PC

Power supply jack

5. Computer Connection

Connect your 210D to your Windows

TM

computer with the provided serial or USB
cable. The cable connection port is located
on the left-hand side of the 210D.

6. Power Supply

Your 210D comes with a universal (115V or 230V) benchtop 12 volt power
supply (shown at right). Plug the small power source cord into the jack on
the left hand side of the LC. Plug the larger power cord into a wall outlet.
Turn the power ON with the power switch, located above the power supply
jack. The 210D can be powered with any regulated or unregulated voltage
in the range of 11-15 volts at 80 watts (approximately 7 amps), including a
car battery via the cigarette lighter.