Shel Lab SRI6PF User Manual
Installation and
Operation Manual
SRI6PF, SRI20PF
Previously Designated
LI6P-FLY, LI20P-FLY
FLY INCUBATORS 100 - 120 Voltage
Sheldon Refrigerated Incubator Peltier FLY
100 - 120 Voltage
Installation and Operation Manual
Part Number (Manual): 4861674-1
Revision: October 9, 2015
Pictured on Cover: SRI6PF Left, SRI20PF Right
These units are TÜV CUE listed as incubators and radiant warmers for professional, industrial, or
educational use where the preparation or testing of materials is done at an ambient air pressure
range of 22.14 – 31.3 inHg (75 – 106 kPa) and no flammable, volatile, or combustible materials are
being heated.
These units have been tested to the following requirements:
CAN/CSA C22.2 No. 61010-1:2012
CAN/CSA C22.2 No. 61010-2-010:2004 Reaffirmed: 2014-07
UL 61010-1:2012-05
UL 61010A-2-010:2002-03
EN 61010-1:2010
EN 61010-2-010:2014
Supplemented by: UL 61010-2-010:2015
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TABLE OF CONTENTS
INTRODUCTION ........................................................................................................................................... 6
General Safety Considerations ................................................................................................................. 6
Engineering Improvements ....................................................................................................................... 7
Contacting Assistance ............................................................................................................................... 7
RECEIVING YOUR INCUBATOR ................................................................................................................ 8
Inspect the Shipment ................................................................................................................................ 8
Orientation Photos..................................................................................................................................... 9
Recording Data Plate Information ........................................................................................................... 11
Reference Sensor Device ....................................................................................................................... 11
INSTALLATION .......................................................................................................................................... 12
Location ................................................................................................................................................... 12
Power Source Requirements .................................................................................................................. 12
Lifting and Handling ................................................................................................................................ 13
Leveling ................................................................................................................................................... 13
Access Port Stopper ............................................................................................................................... 13
Install Incubator in Location .................................................................................................................... 13
Deionized and Distilled Water ................................................................................................................. 13
Installation Cleaning and Disinfection ..................................................................................................... 14
Install Side Air Ducts SRI20PF ............................................................................................................... 14
Shelving Installation ................................................................................................................................ 15
GRAPHIC SYMBOLS ................................................................................................................................. 17
CONTROL PANEL OVERVIEW ................................................................................................................. 18
OPERATION ............................................................................................................................................... 20
Theory of Operation ................................................................................................................................ 20
Preparing the Incubator ........................................................................................................................... 22
Set Current and Cycle Start Times ......................................................................................................... 23
Turn Off the OTL ..................................................................................................................................... 25
Set the Nighttime Temperature Set Point ............................................................................................... 26
Set the Daytime Temperature Set Point ................................................................................................. 27
Temperature Display Accuracy Verification ............................................................................................ 28
Set the Over Temperature Limit .............................................................................................................. 30
Loading the Incubator ............................................................................................................................. 32
Attaching equipment to the Interior Accessory Outlet ............................................................................. 32
Humidifying the Incubator ....................................................................................................................... 32
Managing Condensation ......................................................................................................................... 33
Launching the Auto Cycle ....................................................................................................................... 34
Ending the Auto Cycle ............................................................................................................................. 34
Turn Alarm Off / On ................................................................................................................................. 36
USER MAINTENANCE ............................................................................................................................... 38
Cleaning and Disinfecting ....................................................................................................................... 38
Maintaining Atmospheric Integrity ........................................................................................................... 39
Electrical Components ............................................................................................................................ 39
Calibrate the Temperature Display ......................................................................................................... 40
UNIT SPECIFICATIONS ............................................................................................................................. 47
Weight ..................................................................................................................................................... 47
Dimensions .............................................................................................................................................. 47
Capacity .................................................................................................................................................. 47
Shelf Capacity by Weight ........................................................................................................................ 48
Temperature ............................................................................................................................................ 48
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Power ...................................................................................................................................................... 48
PARTS LIST ............................................................................................................................................... 49
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INTRODUCTION
Thank you for purchasing a Sheldon Refrigerated Peltier Fly Incubator. We know that in today’s
competitive marketplace, customers have many choices when it comes to constant temperature
equipment. We appreciate you choosing ours. Our continued reputation as a leading laboratory
product manufacturer rests with your satisfaction. Sheldon Manufacturing, Inc. stands behind our
products, and we will be here if you need us.
These incubators are intended for professional, industrial, or educational use for fruit fly cultivation.
These units are not designed for use in hazardous or household locations.
Before using the incubator read this entire manual carefully to understand how to install, operate,
and maintain the unit in a safe manner. Keep this manual available for use by all unit operators.
Ensure that all operators are given appropriate training prior to using the incubator.
Note: This unit is designed for the growth, cultivation, incubation, and storage of fruit flies
(Drosophila melanogaster). Improper use of this unit will void the manufacturing defect
warranty. Other units are specifically manufactured for BOD applications. Consult your Shel
Lab dealer or customer service representative in order to identify another model suitable for
your application.
GENERAL SAFETY CONSIDERATIONS
Note: Failure to follow the guidelines and instructions in this manual may create a protection
impairment by disabling or interfering with the unit safety features. This can result in injury
or death.
Your incubator and its recommended accessories are designed and tested to meet strict safety
requirements. Only use this equipment for its intended application. Any alterations or modifications
void the warranty.
For continued safe operation of your incubator always follow basic safety precautions including:
Always plug the unit power cord into a protective earth grounded electrical receptacle
(outlet) that conforms to national and local electrical codes. If the unit is not grounded
properly, parts such as knobs and controls can conduct electricity and cause serious injury.
Avoid damaging the power cord. Do not bend it excessively, step on it, or place heavy
objects on it. A damaged cord can be a shock or fire hazard. Never use a power cord if it is
damaged.
Always position the unit so that end-users can quickly unplug it in the event of an
emergency.
Do not attempt to move the unit while in operation or before the unit has cooled.
Use only approved accessories. Do not modify system components. Any alterations or
modifications to your unit can be dangerous and void your warranty.
Follow all local ordinances in your area regarding the use of this unit. If you have any
questions about local requirements, please contact the appropriate agencies.
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INTRODUCTION (CONTINUED)
ENGINEERING IMPROVEMENTS
Sheldon Manufacturing continually improves all of its products. As a result, engineering changes
and improvements are made from time to time. Therefore, some changes, modifications, and
improvements may not be covered in this manual. If your unit’s operating characteristics or
appearance differs from those described in this manual, please contact your Shel Lab dealer or
distributor for assistance.
CONTACTING ASSISTANCE
If you are unable to resolve a technical issue with your incubator, please contact Sheldon Technical
Support. Phone hours for Sheldon Technical Support are 6 am – 4:30 pm Pacific Coast Time (west
coast of the United States, UTC -8). Please have the following information ready when calling or
emailing Technical Support: the model number and the serial number (see page 11).
EMAIL: tech@shellab.com PHONE: 1-800-322-4897 extension 4, or (503) 640-3000 FAX: (503)
640-1366
Sheldon Manufacturing INC.
P.O. Box 627
Cornelius, OR 97113
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Model
Shelves
Shelf Mounts
Shelf Mounts
SRI6PF
2
4 Standard Brackets
--
SRI20PF
5
8 Standard Brackets
2 Sliding Brackets
Model
Leveling Feet
Access Port Stopper*
Side Air Duct Panels
SRI6PF
4 1 0
SRI20PF
4 1 2
RECEIVING YOUR INCUBATOR
Before leaving the factory, all incubators are packaged in high-quality shipping materials to provide
protection from transportation-related damage. When a unit leaves the factory, safe delivery
becomes the responsibility of the carrier. Damage sustained during transit is not covered by the
warranty.
This makes it important that you inspect your incubator for concealed loss or damage to its interior
and exterior when receiving it. If you find any damage to the unit, follow the carrier’s procedure for
claiming damage or loss.
The orientation photos found on the following pages may serves as a useful visual guide for
inspections.
INSPECT THE SHIPMENT
Carefully inspect the shipping carton for damage. Report any damage to the carrier service that
delivered the incubator. If the carton is not damaged, open the carton and remove the contents.
The unit should come with an Installation and Operation Manual, warranty card, and a Certificate of
Compliance.
Verify that the correct number of the following accessories are present:
*The stopper ships installed in the access port, inside the unit incubation chamber.
The unit also comes with an 115V, 15 Amp, 8 feet (2.5m), NEMA 5-15P power supply cord.
Carefully check all packaging before discarding. Save the shipping carton until you are sure
everything works properly.
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Figure 1: SRI20PF
Control Panel
Temperature
Sensor Probes
Peltier Thermoelectric
Chiller – Heater Housing
Access Port
Chamber
Power Outlet
Drain Port –
TEC-H
Housing
Sliding Shelf
Mount
Shelf
Standard Rail
Door Gasket
Incubation
Chamber Door
Day Phase
Illumination
LED Bar
Back of Unit
Power Cord Inlet
with Fuse
Data Plate
RECEIVING YOUR INCUBATOR (CONTINUED)
ORIENTATION PHOTOS
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Control Panel
Peltier Thermoelectric Chiller
– Heater Housing
Drain Port –
TEC-H Housing
Temperature
Sensor Probes
Chamber
Power Outlet
Incubation
Chamber Door
Door Gasket
Access Port
Day Mode
LED Bar
Figure 2: SRI6PF
Back of Unit
Power Cord Inlet
with Fuse
Data Plate
RECEIVING YOUR INCUBATOR (CONTINUED)
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Model Number
Serial Number
Figure 3: Data Plate
RECEIVING (CONTINUED)
RECORDING DATA PLATE INFORMATION
The data plate contains the incubator model number and serial
number. Record this information for future reference.
The data plate is located on the back of the unit, on the top right side.
Date Plate Information
REFERENCE SENSOR DEVICE
A reference device must be purchased separately for performing temperature display accuracy
verifications and calibrations.
The reference device must be accurate to at least 0.1°C. The device should be regularly calibrated,
preferably by a third party. For best results, use a digital device with a wired-connected temperature
sensing probe that can be placed in the incubation chamber through the access port, or through the
chamber door space, with the door closed over the probe wire. For example, a wire thermocouple
probe.
Reference readings that avoid chamber door openings eliminate significant wait periods required
for the chamber temperature to re-stabilize each time the chamber is disturbed.
Alcohol thermometers do not have sufficient accuracy for conducting accurate temperature
verifications and calibrations. Do not use a mercury thermometer. Never place a mercury
thermometer in the incubation chamber.
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INSTALLATION
This incubator is intended for use indoors, at room temperatures between 15C and 30C (59F
and 86F), at no greater than 80% Relative Humidity at 25C (77F). Allow a minimum of 4
inches (10cm) between the incubator and walls or partitions and 2 inches (5cm) of clearance
above the top of the incubator for unobstructed airflow. Position the unit so the end-user has
access to the power plug.
Operating the unit outside these conditions may adversely affect its temperature range and stability.
For conditions outside of those listed above, please contact your distributor to explore other unit
options suited to your laboratory or production environment.
LOCATION
When selecting a location to install the incubator, consider all environmental conditions that can
affect the effective temperature range, uniformity, and stability of the unit. For example:
Ovens, autoclaves, and any device that produces significant radiant heat
Heating and cooling ducts, or other sources of fast moving air currents
High-traffic areas
Direct sunlight
POWER SOURCE REQUIREMENTS
When selecting a location for the unit, verify that each of the following requirements are satisfied:
Wall power sources must match the voltage and ampere requirements listed on the unit
data plate. These units are intended for 100 – 120 VAC 50/60 Hz applications at the
following amperages: SRI20PF 5.5 Amps; SRI6PF 4.0 Amps.
Supplied voltage must not vary more than 10% from the data plate rating. Damage to
the unit may result if supplied voltage varies more than 10%.
The wall power source must conform to all national and local electrical codes.
Wall power sources must be protective earth grounded. Use a separate circuit to prevent
loss of product due to overloading or circuit failure.
Each SRIPF is provided with a 250V, T6.3A, 5X20MM fuse located in the power cord inlet
on the back of the incubator.
The unit must be positioned so that all end-users can quickly unplug the power cord in the
event of an emergency.
The unit comes provided with a 125 volt, 15Amp, 9ft 5 in (2.86m) NEMA 5-15P power cord.
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Figure 4: Leveling Foot
and Port Stopper
INSTALLATION (CONTINUED)
LIFTING AND HANDLING
An SRIPF Incubator should only be lifted by its bottom surfaces using proper heavy lifting
machinery such as a forklift or pallet jack. Handles and knobs are inadequate for lifting or
stabilization. The unit should be completely restrained from tipping during lifting. Transporting the
unit while lifted is not recommended and may be hazardous. Remove all moving parts, such as
shelves and trays, and secure the door in the closed position prior to lifting the unit.
Do not attempt to move the unit while in operation or before the unit has cooled.
LEVELING
The unit must be level and stable for safe operation Each incubator ships with
four leveling feet. Insert one leveling foot into each of the four holes in the
bottom corners of the unit. Adjust the foot at each corner until the unit stands
level and solid without rocking. To raise a foot, turn it in a counterclockwise
direction; to lower a foot, turn it in a clockwise direction.
To prevent damage to the feet while in transport, turn all the feet to the
maximum counterclockwise position prior to transport.
ACCESS PORT STOPPER
Each SRIPF incubator is provided with an access port located on the back
wall of the incubation chamber. The incubator is shipped with one rubber
access port stopper installed in the port, inside the chamber. The stopper
should always be installed inside the chamber to obtain the best
temperature uniformity and prevent condensation from forming inside the
port. A second stopper may be installed on the outside of the unit to
prevent dust from building up in the port, but is not required.
Wires for thermocouples and other sensor probes may be introduced into
the chamber through the access port. The stopper may be put in place
over the wires.
The port stopper must be in place during operation inorder for the
incubation chamber to achieve its specified temperature stability and
uniformity levels.
INSTALL INCUBATOR IN LOCATION
Install the unit in a workspace location that meets the criteria discussed in the
previous entries of the Installation section.
DEIONIZED AND DISTILLED WATER
Do not use deionized water to clean the incubator. Use of deionized water may corrode metal
surfaces and voids the warranty. Sheldon Manufacturing recommends the use of distilled water in
the resistance range of 50K Ohm/cm to 1M Ohm/cm, or a conductivity range of 20.0 uS/cm to 1.0
uS/cm, for cleaning applications.
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INSTALLATION (CONTINUED)
INSTALLATION CLEANING AND DISINFECTION
If required by your laboratory protocol, clean and manually disinfect the incubator chamber and
shelving components. Cleaning and disinfecting during installation reduces the risk of
contamination. The chamber and shelving were cleaned at the factory, however, Sheldon
Manufacturing cannot guarantee that the incubator was not exposed to contaminants during
shipping.
Remove all wrappings and coverings from shelving (and the air ducts of the SRI20P-2) prior to
cleaning and installation.
Please see the Cleaning and Disinfecting entry on page 38 in the User Maintenance section for
information on how to clean and disinfect without damaging the incubator or its components. A
decontamination cycle will be run as part of the Incubator set up in the Operation section.
INSTALL SIDE AIR DUCTS SRI20PF
Two (2) side air duct panels are included in the SRI20PF accessories. Hang the panels on the
chamber side walls, by placing the hooks on the top and bottom corners of the panels into the slots
provided on the shelf standard mounting rails. The SRI6PF incubator does not require side ducts.
Figure 5: SRI20PF
Air Duct Panel
Note: The air duct panels play an important role maintaining even heat distribution inside the long
incubation chamber of the SRI20PF. Failure to install both air duct panels may adversely
impact the incubator’s temperature uniformity.
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Figure 7: Standard Mounting
Bracket Installed
Figure 8: Standard Shelf Installed
on Mounting Brackets
INSTALLATION (CONTINUED)
SHELVING INSTALLATION
SRI6PF and SRI20PF Standard Shelving Installation
Perform the following steps to install the SRI6PF and SRI20PF incubator standard shelves:
Figure 6: Standard Shelf Mounting Bracket Installation
1. Insert the 2 pairs of tabs on the bracket (front and back) into the slots on the shelf standard
rails of the incubation chamber.
2. Slide the bracket down so that the tabs are securely seated in the mounting slots.
3. Repeat the process on the opposite side of the chamber with the second mounting bracket.
4. Install 1 shelf on the 2 mounting brackets.
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