Installation and
Operation Manual
SRI6PF, SRI20PF
Previously Designated
LI6P-FLY, LI20P-FLY
FLY INCUBATORS 100 - 120 Voltage
Sheldon Refrigerated Incubator Peltier FLY
100 - 120 Voltage
Installation and Operation Manual
Part Number (Manual): 4861674-1
Revision: October 9, 2015
Pictured on Cover: SRI6PF Left, SRI20PF Right
These units are TÜV CUE listed as incubators and radiant warmers for professional, industrial, or
educational use where the preparation or testing of materials is done at an ambient air pressure
range of 22.14 – 31.3 inHg (75 – 106 kPa) and no flammable, volatile, or combustible materials are
being heated.
These units have been tested to the following requirements:
CAN/CSA C22.2 No. 61010-1:2012
CAN/CSA C22.2 No. 61010-2-010:2004 Reaffirmed: 2014-07
UL 61010-1:2012-05
UL 61010A-2-010:2002-03
EN 61010-1:2010
EN 61010-2-010:2014
Supplemented by: UL 61010-2-010:2015
2 | P a g e
This page left blank
3 | P a g e
TABLE OF CONTENTS
INTRODUCTION ........................................................................................................................................... 6
General Safety Considerations ................................................................................................................. 6
Engineering Improvements ....................................................................................................................... 7
Contacting Assistance ............................................................................................................................... 7
RECEIVING YOUR INCUBATOR ................................................................................................................ 8
Inspect the Shipment ................................................................................................................................ 8
Orientation Photos..................................................................................................................................... 9
Recording Data Plate Information ........................................................................................................... 11
Reference Sensor Device ....................................................................................................................... 11
INSTALLATION .......................................................................................................................................... 12
Location ................................................................................................................................................... 12
Power Source Requirements .................................................................................................................. 12
Lifting and Handling ................................................................................................................................ 13
Leveling ................................................................................................................................................... 13
Access Port Stopper ............................................................................................................................... 13
Install Incubator in Location .................................................................................................................... 13
Deionized and Distilled Water ................................................................................................................. 13
Installation Cleaning and Disinfection ..................................................................................................... 14
Install Side Air Ducts SRI20PF ............................................................................................................... 14
Shelving Installation ................................................................................................................................ 15
GRAPHIC SYMBOLS ................................................................................................................................. 17
CONTROL PANEL OVERVIEW ................................................................................................................. 18
OPERATION ............................................................................................................................................... 20
Theory of Operation ................................................................................................................................ 20
Preparing the Incubator ........................................................................................................................... 22
Set Current and Cycle Start Times ......................................................................................................... 23
Turn Off the OTL ..................................................................................................................................... 25
Set the Nighttime Temperature Set Point ............................................................................................... 26
Set the Daytime Temperature Set Point ................................................................................................. 27
Temperature Display Accuracy Verification ............................................................................................ 28
Set the Over Temperature Limit .............................................................................................................. 30
Loading the Incubator ............................................................................................................................. 32
Attaching equipment to the Interior Accessory Outlet ............................................................................. 32
Humidifying the Incubator ....................................................................................................................... 32
Managing Condensation ......................................................................................................................... 33
Launching the Auto Cycle ....................................................................................................................... 34
Ending the Auto Cycle ............................................................................................................................. 34
Turn Alarm Off / On ................................................................................................................................. 36
USER MAINTENANCE ............................................................................................................................... 38
Cleaning and Disinfecting ....................................................................................................................... 38
Maintaining Atmospheric Integrity ........................................................................................................... 39
Electrical Components ............................................................................................................................ 39
Calibrate the Temperature Display ......................................................................................................... 40
UNIT SPECIFICATIONS ............................................................................................................................. 47
Weight ..................................................................................................................................................... 47
Dimensions .............................................................................................................................................. 47
Capacity .................................................................................................................................................. 47
Shelf Capacity by Weight ........................................................................................................................ 48
Temperature ............................................................................................................................................ 48
4 | P a g e
Power ...................................................................................................................................................... 48
PARTS LIST ............................................................................................................................................... 49
5 | P a g e
INTRODUCTION
Thank you for purchasing a Sheldon Refrigerated Peltier Fly Incubator. We know that in today’s
competitive marketplace, customers have many choices when it comes to constant temperature
equipment. We appreciate you choosing ours. Our continued reputation as a leading laboratory
product manufacturer rests with your satisfaction. Sheldon Manufacturing, Inc. stands behind our
products, and we will be here if you need us.
These incubators are intended for professional, industrial, or educational use for fruit fly cultivation.
These units are not designed for use in hazardous or household locations.
Before using the incubator read this entire manual carefully to understand how to install, operate,
and maintain the unit in a safe manner. Keep this manual available for use by all unit operators.
Ensure that all operators are given appropriate training prior to using the incubator.
Note: This unit is designed for the growth, cultivation, incubation, and storage of fruit flies
(Drosophila melanogaster). Improper use of this unit will void the manufacturing defect
warranty. Other units are specifically manufactured for BOD applications. Consult your Shel
Lab dealer or customer service representative in order to identify another model suitable for
your application.
GENERAL SAFETY CONSIDERATIONS
Note: Failure to follow the guidelines and instructions in this manual may create a protection
impairment by disabling or interfering with the unit safety features. This can result in injury
or death.
Your incubator and its recommended accessories are designed and tested to meet strict safety
requirements. Only use this equipment for its intended application. Any alterations or modifications
void the warranty.
For continued safe operation of your incubator always follow basic safety precautions including:
Always plug the unit power cord into a protective earth grounded electrical receptacle
(outlet) that conforms to national and local electrical codes. If the unit is not grounded
properly, parts such as knobs and controls can conduct electricity and cause serious injury.
Avoid damaging the power cord. Do not bend it excessively, step on it, or place heavy
objects on it. A damaged cord can be a shock or fire hazard. Never use a power cord if it is
damaged.
Always position the unit so that end-users can quickly unplug it in the event of an
emergency.
Do not attempt to move the unit while in operation or before the unit has cooled.
Use only approved accessories. Do not modify system components. Any alterations or
modifications to your unit can be dangerous and void your warranty.
Follow all local ordinances in your area regarding the use of this unit. If you have any
questions about local requirements, please contact the appropriate agencies.
6 | P a g e
INTRODUCTION (CONTINUED)
ENGINEERING IMPROVEMENTS
Sheldon Manufacturing continually improves all of its products. As a result, engineering changes
and improvements are made from time to time. Therefore, some changes, modifications, and
improvements may not be covered in this manual. If your unit’s operating characteristics or
appearance differs from those described in this manual, please contact your Shel Lab dealer or
distributor for assistance.
CONTACTING ASSISTANCE
If you are unable to resolve a technical issue with your incubator, please contact Sheldon Technical
Support. Phone hours for Sheldon Technical Support are 6 am – 4:30 pm Pacific Coast Time (west
coast of the United States, UTC -8). Please have the following information ready when calling or
emailing Technical Support: the model number and the serial number (see page 11).
EMAIL: tech@shellab.com PHONE: 1-800-322-4897 extension 4, or (503) 640-3000 FAX: (503)
640-1366
Sheldon Manufacturing INC.
P.O. Box 627
Cornelius, OR 97113
7 | P a g e
Model
Shelves
Shelf Mounts
Shelf Mounts
SRI6PF
2
4 Standard Brackets
--
SRI20PF
5
8 Standard Brackets
2 Sliding Brackets
Model
Leveling Feet
Access Port Stopper*
Side Air Duct Panels
SRI6PF
4 1 0
SRI20PF
4 1 2
RECEIVING YOUR INCUBATOR
Before leaving the factory, all incubators are packaged in high-quality shipping materials to provide
protection from transportation-related damage. When a unit leaves the factory, safe delivery
becomes the responsibility of the carrier. Damage sustained during transit is not covered by the
warranty.
This makes it important that you inspect your incubator for concealed loss or damage to its interior
and exterior when receiving it. If you find any damage to the unit, follow the carrier’s procedure for
claiming damage or loss.
The orientation photos found on the following pages may serves as a useful visual guide for
inspections.
INSPECT THE SHIPMENT
Carefully inspect the shipping carton for damage. Report any damage to the carrier service that
delivered the incubator. If the carton is not damaged, open the carton and remove the contents.
The unit should come with an Installation and Operation Manual, warranty card, and a Certificate of
Compliance.
Verify that the correct number of the following accessories are present:
*The stopper ships installed in the access port, inside the unit incubation chamber.
The unit also comes with an 115V, 15 Amp, 8 feet (2.5m), NEMA 5-15P power supply cord.
Carefully check all packaging before discarding. Save the shipping carton until you are sure
everything works properly.
8 | P a g e
Figure 1: SRI20PF
Control Panel
Temperature
Sensor Probes
Peltier Thermoelectric
Chiller – Heater Housing
Access Port
Chamber
Power Outlet
Drain Port –
TEC-H
Housing
Sliding Shelf
Mount
Shelf
Standard Rail
Door Gasket
Incubation
Chamber Door
Day Phase
Illumination
LED Bar
Back of Unit
Power Cord Inlet
with Fuse
Data Plate
RECEIVING YOUR INCUBATOR (CONTINUED)
ORIENTATION PHOTOS
9 | P a g e
Control Panel
Peltier Thermoelectric Chiller
– Heater Housing
Drain Port –
TEC-H Housing
Temperature
Sensor Probes
Chamber
Power Outlet
Incubation
Chamber Door
Door Gasket
Access Port
Day Mode
LED Bar
Figure 2: SRI6PF
Back of Unit
Power Cord Inlet
with Fuse
Data Plate
RECEIVING YOUR INCUBATOR (CONTINUED)
10 | P a g e
Model Number
Serial Number
Figure 3: Data Plate
RECEIVING (CONTINUED)
RECORDING DATA PLATE INFORMATION
The data plate contains the incubator model number and serial
number. Record this information for future reference.
The data plate is located on the back of the unit, on the top right side.
Date Plate Information
REFERENCE SENSOR DEVICE
A reference device must be purchased separately for performing temperature display accuracy
verifications and calibrations.
The reference device must be accurate to at least 0.1°C. The device should be regularly calibrated,
preferably by a third party. For best results, use a digital device with a wired-connected temperature
sensing probe that can be placed in the incubation chamber through the access port, or through the
chamber door space, with the door closed over the probe wire. For example, a wire thermocouple
probe.
Reference readings that avoid chamber door openings eliminate significant wait periods required
for the chamber temperature to re-stabilize each time the chamber is disturbed.
Alcohol thermometers do not have sufficient accuracy for conducting accurate temperature
verifications and calibrations. Do not use a mercury thermometer. Never place a mercury
thermometer in the incubation chamber.
11 | P a g e
INSTALLATION
This incubator is intended for use indoors, at room temperatures between 15C and 30C (59F
and 86F), at no greater than 80% Relative Humidity at 25C (77F). Allow a minimum of 4
inches (10cm) between the incubator and walls or partitions and 2 inches (5cm) of clearance
above the top of the incubator for unobstructed airflow. Position the unit so the end-user has
access to the power plug.
Operating the unit outside these conditions may adversely affect its temperature range and stability.
For conditions outside of those listed above, please contact your distributor to explore other unit
options suited to your laboratory or production environment.
LOCATION
When selecting a location to install the incubator, consider all environmental conditions that can
affect the effective temperature range, uniformity, and stability of the unit. For example:
Ovens, autoclaves, and any device that produces significant radiant heat
Heating and cooling ducts, or other sources of fast moving air currents
High-traffic areas
Direct sunlight
POWER SOURCE REQUIREMENTS
When selecting a location for the unit, verify that each of the following requirements are satisfied:
Wall power sources must match the voltage and ampere requirements listed on the unit
data plate. These units are intended for 100 – 120 VAC 50/60 Hz applications at the
following amperages: SRI20PF 5.5 Amps; SRI6PF 4.0 Amps.
Supplied voltage must not vary more than 10% from the data plate rating. Damage to
the unit may result if supplied voltage varies more than 10%.
The wall power source must conform to all national and local electrical codes.
Wall power sources must be protective earth grounded. Use a separate circuit to prevent
loss of product due to overloading or circuit failure.
Each SRIPF is provided with a 250V, T6.3A, 5X20MM fuse located in the power cord inlet
on the back of the incubator.
The unit must be positioned so that all end-users can quickly unplug the power cord in the
event of an emergency.
The unit comes provided with a 125 volt, 15Amp, 9ft 5 in (2.86m) NEMA 5-15P power cord.
12 | P a g e
Figure 4: Leveling Foot
and Port Stopper
INSTALLATION (CONTINUED)
LIFTING AND HANDLING
An SRIPF Incubator should only be lifted by its bottom surfaces using proper heavy lifting
machinery such as a forklift or pallet jack. Handles and knobs are inadequate for lifting or
stabilization. The unit should be completely restrained from tipping during lifting. Transporting the
unit while lifted is not recommended and may be hazardous. Remove all moving parts, such as
shelves and trays, and secure the door in the closed position prior to lifting the unit.
Do not attempt to move the unit while in operation or before the unit has cooled.
LEVELING
The unit must be level and stable for safe operation Each incubator ships with
four leveling feet. Insert one leveling foot into each of the four holes in the
bottom corners of the unit. Adjust the foot at each corner until the unit stands
level and solid without rocking. To raise a foot, turn it in a counterclockwise
direction; to lower a foot, turn it in a clockwise direction.
To prevent damage to the feet while in transport, turn all the feet to the
maximum counterclockwise position prior to transport.
ACCESS PORT STOPPER
Each SRIPF incubator is provided with an access port located on the back
wall of the incubation chamber. The incubator is shipped with one rubber
access port stopper installed in the port, inside the chamber. The stopper
should always be installed inside the chamber to obtain the best
temperature uniformity and prevent condensation from forming inside the
port. A second stopper may be installed on the outside of the unit to
prevent dust from building up in the port, but is not required.
Wires for thermocouples and other sensor probes may be introduced into
the chamber through the access port. The stopper may be put in place
over the wires.
The port stopper must be in place during operation inorder for the
incubation chamber to achieve its specified temperature stability and
uniformity levels.
INSTALL INCUBATOR IN LOCATION
Install the unit in a workspace location that meets the criteria discussed in the
previous entries of the Installation section.
DEIONIZED AND DISTILLED WATER
Do not use deionized water to clean the incubator. Use of deionized water may corrode metal
surfaces and voids the warranty. Sheldon Manufacturing recommends the use of distilled water in
the resistance range of 50K Ohm/cm to 1M Ohm/cm, or a conductivity range of 20.0 uS/cm to 1.0
uS/cm, for cleaning applications.
13 | P a g e
INSTALLATION (CONTINUED)
INSTALLATION CLEANING AND DISINFECTION
If required by your laboratory protocol, clean and manually disinfect the incubator chamber and
shelving components. Cleaning and disinfecting during installation reduces the risk of
contamination. The chamber and shelving were cleaned at the factory, however, Sheldon
Manufacturing cannot guarantee that the incubator was not exposed to contaminants during
shipping.
Remove all wrappings and coverings from shelving (and the air ducts of the SRI20P-2) prior to
cleaning and installation.
Please see the Cleaning and Disinfecting entry on page 38 in the User Maintenance section for
information on how to clean and disinfect without damaging the incubator or its components. A
decontamination cycle will be run as part of the Incubator set up in the Operation section.
INSTALL SIDE AIR DUCTS SRI20PF
Two (2) side air duct panels are included in the SRI20PF accessories. Hang the panels on the
chamber side walls, by placing the hooks on the top and bottom corners of the panels into the slots
provided on the shelf standard mounting rails. The SRI6PF incubator does not require side ducts.
Figure 5: SRI20PF
Air Duct Panel
Note: The air duct panels play an important role maintaining even heat distribution inside the long
incubation chamber of the SRI20PF. Failure to install both air duct panels may adversely
impact the incubator’s temperature uniformity.
14 | P a g e
Figure 7: Standard Mounting
Bracket Installed
Figure 8: Standard Shelf Installed
on Mounting Brackets
INSTALLATION (CONTINUED)
SHELVING INSTALLATION
SRI6PF and SRI20PF Standard Shelving Installation
Perform the following steps to install the SRI6PF and SRI20PF incubator standard shelves:
Figure 6: Standard Shelf Mounting Bracket Installation
1. Insert the 2 pairs of tabs on the bracket (front and back) into the slots on the shelf standard
rails of the incubation chamber.
2. Slide the bracket down so that the tabs are securely seated in the mounting slots.
3. Repeat the process on the opposite side of the chamber with the second mounting bracket.
4. Install 1 shelf on the 2 mounting brackets.
15 | P a g e
Figure 10: Sliding Shelf
Mounting Bracket Installation
Figure 9: Sliding Shelf Mounting
Bracket Installation
Figure 12: Sliding Mounting Bracket Screws
Figure 11: Sliding Mounting Bracket Front
Screw Installed
INSTALLATION (CONTINUED)
SRI6PF and SRI20PF Sliding Shelf Installation
Note: The SRI6PF does not come with a sliding shelf mounts. Sliding mounts for the SRI6PF must
be purchased separately
1. Insert the sliding bracket’s two pairs of tabs (front and back) into the slots on the shelf
standards. See Figure 10.
2. Slide the bracket down so that the tabs are securely seated in the mounting slots. See
Figure 9.
3. Secure the bottom flange of the sliding bracket to the shelf standard rails by threading and
screwing in 2 screws, on the front and back of the flange. See Figures 11 and 12.
4. Repeat the process on the opposite side of the chamber with the second sliding mounting
bracket.
5. Install 1 shelf on the 2 sliding mounting brackets.
16 | P a g e
Symbol
Definition
Indicates that you should consult your user manual for further instructions.
Indique que l'opérateur doit consulter le manuel d'utilisation pour y trouver les instructions
complémentaires.
Indicates Temperature
Repère température
Indicates the Over Temperature Limit system
Indique le système de dépassement de temperature
Indicates AC Power
Repère le courant alternatif
Indicates I/ON and O/OFF
I repère de la position MARCHE de l'interrupteur d'alimentation
O repère de la position ARRÊT de l'interrupteur d'alimentation
Indicates protective earth ground
Repère terre électrique
Indicates UP and DOWN respectively
Touches de déplacements respectifs vers le HAUT et le BA
Indicates Manually Adjustable
Indique un bouton réglable manuellement
Indicates Potential Shock Hazard
Signale danger électrique
Indicates the unit should be recycled (Not disposed of in land-fill)
Indique l’appareil doit être recyclé (Ne pas jeter dans une décharge)
GRAPHIC SYMBOLS
The incubator is provided with graphic symbols on its interior and exterior surfaces. The symbols
identify hazards and the functions of the adjustable components, as well as important notes in the
user manual.
17 | P a g e
Figure 13: SRI6PF and SRI20PF Control Panel
CONTROL PANEL OVERVIEW
Power Switch
The green power switch on the panel controls all power to the unit and its systems. The switch
illuminates when in the On position ( I ).
Time Display
The green LED display labeled TIME on the left side of the panel operates as a 24-hour clock
(00:00 – 23:59). During normal operations, it displays the current time. When setting the start times
of the automatic day – night illumination and temperature cycle it shows the Daytime start time
and the Nighttime start time separately. Three LED lights immediately below the Time screen
indicate the present mode of display: Current Time, adjustable Daytime start time, and the
adjustable Nighttime start time.
Temperature Display
Marked SET TEMPERATURE, this digital display shows the air temperature in the incubation
chamber, accurate to 0.1°C. The display can also show the adjustable temperature set points of the
Day and Night Modes, as well as display value adjustments during temperature calibrations.
Just below the Temperature Display are two LED lights, Day Temp and Night Temp. These
indicate which mode the incubator is running in (Day or Night), as well as which mode’s
temperature set point is being displayed when adjusting the set points.
Temperature and Timer Control Arrows
Temperature functions: The arrow buttons are used to adjust the temperature set points of the
Day and Night Modes. When the display is in its temperature calibration menu, the arrow keys are
used to make calibration adjustments. Time functions: When the time display is in the Time Set
Up menu, the arrow buttons adjust the Current Time, then the auto cycle Daytime and Nighttime
start times.
18 | P a g e
CONTROL PANEL (CONTINUED)
Enter Button
The ENTER button is used to save adjustments to the Current Time as well as the auto cycle Day
and Night start times while in the Time Set Up menu. The Enter button can also be used to scroll
forward through the menu.
Mode Button
Pressing and immediately releasing the button enters the Time Setup menu. Pressing and holding
the Mode button for 5 seconds switches the incubator between the Daytime and Nighttime modes
when the day – night auto cycle is not running.
Cycle Start Button
Pressing and holding this button for 2 seconds will launch the day – night auto cycle, or terminate
the cycle if it is running. The green PROGRAM ACTIVE indicator to the right of the button
illuminates when the auto cycle is running.
Heating and Cooling Light
The green pilot light located beneath the label TEC ACTIVATED illuminates whenever the Peltier
TEC-H device is chilling or heating the chamber. The light will be on almost continually during
normal operations as the unit makes continual, small temperature compensations.
Set Over Temperature
The OTL is a mechanical heating cut off system that operates independently of the digital
temperature controller. It guards against failures of the digital controller that would allow the
chamber temperature to rise past the controller set point. Please see the Over Temperature Limit
System description in the Theory of Operations section (page 21) for a complete explanation.
OTL Light
The red pilot light marked OVER TEMPERATURE ACTIVATED illuminates when the Over
Temperature Limit system cuts power to the Peltier TEC heating circuits. Under normal operating
conditions this indicator should never turn on.
19 | P a g e
OPERATION
THEORY OF OPERATION
The SRIPF family of fly incubators is designed to provide a variable temperature and illumination
environment suitable for the cultivation and storage of fruit flies (Drosophila melanogaster). The
incubator runs in one of two modes, Daytime and Nighttime. In its Daytime mode, the incubator
provides continual illumination in the incubation chamber using two side-mounted white light LED
bars. In Nighttime Mode, the chamber is left dark though a small LED bar at the top of the chamber
will illuminate whenever the chamber door is opened.
The Day and Night Modes have independent, programmable temperature set points. Both are set
to 20°C at the factory, but can be adjusted to different temperatures in order provide a thermal
variation between the modes.
The SRIPF incubator comes from the factory set to run indefinitely in the Daytime Mode. Users can
manually switch between it and the Nighttime Mode by pressing and holding the MODE button. The
incubator is also provided with a Day – Night Auto Cycle, which when launched cycles the between
the two modes until manually terminated. As set at the factory, the cycle transitions between modes
are 12 hours apart. These start time points can be adjusted using the incubator controls.
During Day Mode operations the unit microprocessor controller automatically compensates for a
small amount of extra heat generated by the active LED light bars in the incubation chamber.
Heating and Cooling
Conventional low-temperature capable incubators rely on a competition between a refrigeration
compressor and a resistive heating element to achieve stable chamber temperatures. SRIPF
incubators employ an alternative, power saving thermoelectric cooling-and-heating device.
The TEC-H operates using the Peltier effect, in which the junctures between two electrified
conductive plates generate a gradient of temperature differences. In other words, a current
between the conductors produces a flow of heat away from one plate to the other. The direction of
this gradient can be flipped by reversing the direction of the current. The sandwiched TEC
conductors effectively operate as a reversible heat pump that is significantly more efficient than a
compressor – element pairing. This power use efficiency results in far less waste heat and a
corresponding reduction in cooling demand placed on laboratory air conditioning systems.
Heating and cooling of the incubation chamber are controlled by a microprocessor controller. The
controller stores the Daytime mode and Nighttime mode temperature set points. The controller
monitors the air temperature of the incubation chamber using a solid state sensor probe located in
the chamber airstream. When an air temperature deviation from the active set point is detected, the
controller pulses power to the TEC-H device to add or remove heat from the chamber. Heated or
chilled air is circulated through the chamber using a fan attached to the TEC-H.
The controller employs proportional-integral-derivative (PID) analytical feedback-loop functions
when measuring and controlling the chamber temperature. The intensity of PID-controlled heating
or chilling is proportional to the difference between the measured chamber temperature and the
current temperature set point. The frequency of heating or chilling pulses is derived from the rate of
change in the difference. Integrator feedback slows the rate of heating or chilling as the chamber
temperature approaches the set point, which helps prevent overshoots.
20 | P a g e
OPERATION (CONTINUED)
During normal operations, with the chamber door closed, the Peltier device pulses heat or cold to
the chamber almost continuously, as indicated the green TEC Activated pilot light. These are short,
low-power pulses to compensate for deviations of a hundredth degree Celsius (±0.01°C).
As a solid state device, the Peltier TEC-H offers significant maintenance savings cost. The device
can be replaced by a service technician using a screwdriver and a nut driver. It does not require a
certified refrigeration specialist with refrigeration tools, replacement coolant fluid, and does not
contain a system of easily deformed, pressurized fluid vessels.
Door Alarm
Leaving the door open for extended periods disrupts the temperature uniformity and stability of the
incubation chamber, and may result in significantly increased power use as the controller attempts
to compensate for the disruption. The SRIPF incubator is equipped with a magnetic induction door
alarm, which activates when the door is opened and its two sensor components are moved out of
range of one another for 60 seconds. When the alarm is active, an audio alert will sound and the
control panel display will flash. The alarm may be turned off using the Arrow buttons. Please see
the Turn Alarm Off / On procedure on page 36.
Accessory Compatibility
Make sure that any accessory equipment you will be using inside an SRIPF incubator can safely
operate within your selected temperature range. Powered accessories such as, stirrers or shakers
that produce significant waste heat may interfere with the temperature uniformity and operating
range of the chamber. Additionally, fruit flies can produce aerosolized acidic metabolic byproducts
that may affect the long-term performance of accessory equipment.
The Over Temperature Limit System
When set, the OTL system prevents runaway heating in the event of a failure of the main
temperature microprocessor or its sensor probe. It does so by cutting off the current through the
Peltier TEC device in the direction that transmits heat into the incubation chamber. This cutoff
allows the device’s refrigeration function to continue operating without interruption and takes place
whenever the temperature in the cultivation chamber exceeds the OTL setting. The OTL is provided
with an independent temperature probe.
The system is set by the end-user at approximately 1°C above the current chamber temperature,
typically when operating stabilized at the warmest application temperature. This is typically when
the unit has been functioning in the Day Mode for several hours. Setting the over temperature limit
while the unit is operating in the Night Mode or phase risks tripping the OTL when the unit
transitions to a warmer day temperature.
Because of its nature as a cutoff and its lack of PID analytics, the OTL cannot deliver the same
degree of temperature stability and measurement precision as the digital display and controls. The
OTL System should only be used as a means of heating regulation for the cultivation chamber until
a failed processor board and its temperature probe can be repaired or replaced.
The OTL will not prevent a rise in heat caused by a complete failure of the Peltier TEC-H itself. With
the loss of the refrigeration function, the chamber temperature will rise to the ambient room
temperature plus 1 or 2°C.
21 | P a g e
OPERATION (CONTINUED)
PREPARING THE INCUBATOR
Setting up the incubator for use in a new workspace environment requires an 8-hour period for the
unit to come up to and stabilize at the set point temperature, prior to loading the incubation
chamber with samples (step 5e below). During this period, the chamber must be powered
continually, and the chamber door closed. Allowing time for stabilization helps protect samples. It is
also necessary in order to perform the optional Temperature Display Accuracy Verification
procedure (step 5f), as well as any resulting display calibration.
Perform the following steps and procedures to prepare the incubator for use each time it is installed
in a new location:
1. Optional: A clean and disinfected thermocouple probe for performing the optional
temperature display accuracy verification may be inserted through door space into the
cultivation chamber now. This saves time by allowing the unit chamber temperature to
stabilize undisturbed prior to the verification procedure.
a. See the Temperature Display Accuracy Verification procedure on page 28
for the correct introduction and placement of the thermocouple probe.
2. Verify that the workspace power supply and the chamber power requirements listed on the
unit data plate have been matched.
a. See the Power Source Requirement entry on page 12.
3. Plug in the power cord.
a. Plug the female end of the supplied power cord into the inlet on the incubator.
b. Plug the male end into the workspace supply receptacle (outlet).
4. Place the Power switch in the on ( I ) position.
a. The controller display will illuminate. The unit comes from the factory set to run
in manual Night Mode with a set point of 20°C.
5. Perform the following procedures in the Operation section to finish preparing the chamber:
a. Set the Current and Cycle Start Times page 23
b. Turn Off the OTL page 25
c. Set the Night Mode Temperature Set Point page 26
d. Set the Day Mode Temperature Set Point page 27
e. Allow the incubator to operate undisturbed for 8 hours to
stabilize thermally before continuing. This also prepares
the unit for the optional verification procedure below.
22 | P a g e
f. Optional: Verify Temperature Display Accuracy page 28
g. Set the Over Temperature Limit Control page 30
h. Load the Chamber page 32
1. Press and release the Mode button to enter the Time Set Up menu.
a. The Current LED will stay lit, and the Temperature display will
turn off
b. The Time display will show a blinking, adjustable time setting.
TIME
Factory Current Time
2. Adjust the Current time to match your local time using the UP and DOWN
arrows.
a. 24-hour clock afternoon and evening times are 12 hours
greater than the 12-hour clock PM equivalents. Example: 1
PM = 13:00; 6 PM = 18:00; 9 PM = 21:00. 12 AM = 00:00.
Note: Skip to Step 3 if you do not wish to adjust the current clock time.
TIME
Adjusted Clock Time
3. Save the adjusted Current time.
a. Press and release the Enter button.
b. This saves the flashing time shown on the display as the new
Current time.
TIME
Adjusted Time Saved
4. The display will automatically advance to the auto cycle Daytime Start
Time, after pressing Enter.
a. Time Display continues to flash but now displays the
adjustable Daytime start time.
b. The Current Time indicator LED will extinguish, the Daytime
LED indicator will illuminate.
TIME
Cycle Daytime Start:
Factory Setting
OPERATION (CONTINUED)
SET CURRENT AND CYCLE START TIMES
This procedure sets the incubator clock time to your local time, then allows you to adjust the Auto
Cycle start times for the Day and Night modes. The incubator comes from the factory set to US
Pacific Time. The Auto Cycle Daytime start comes from the factory set to 08:00 (8 am), and the
Nighttime start is 20:00 (8 pm).
Procedure continued on next page
23 | P a g e
Set Times (Continued)
5. Use the UP and DOWN arrow keys to adjust the cycle Daytime Start
Time.
Note: Skip to Step 6 if you do not wish to change the Daytime Start time.
Adjusted Daytime Start
6. Save the adjusted cycle Day Start time.
a. Press the Enter button after adjusting the Day Start Time.
Adjusted Time Saved
7. The display will automatically advance to the auto cycle Nighttime Start
Time, after pressing Enter.
a. The Daytime indicator LED will extinguish, and the Night Time
indicator will illuminate.
b. The flashing Time Display will now show the adjustable
Nighttime Start Time.
TIME
Cycle Nighttime Start:
Factory Setting
8. Use the UP and DOWN arrow keys to adjust the Nighttime Start Time.
a. 24-hour clock afternoon and evening times are 12 hours
greater than the 12-hour clock PM equivalents. Example: 1
PM = 13:00; 6 PM = 18:00; 9 PM = 21:00. 12 AM = 00:00.
Note: Skip to Step 9 if you do not wish to change the Nighttime start time.
TIME
Adjusted Night Start
9. Save the adjusted cycle Night Start Time.
a. Press Enter after adjusting the Night Start Time.
TIME
Adjusted Time Saved
10. The display will exit the Time Set Up menu and resume showing the
Current time, after pressing Enter.
a. The Time Display will cease blinking.
b. The Temperature Display will turn on.
c. The Nighttime LED will extinguish, and the Current time (Day)
LED will illuminate.
TIME
Returned to Current Time
Display
OPERATION (CONTINUED)
24 | P a g e
End of procedure
OPERATION (CONTINUED)
Note: The OTL should ship from the factory set to the maximum position.
TURN OFF THE OTL
Always set the OTL dial to its maximum position prior to adjusting the Day temperature mode. This
prevents the OTL system from interfering with raising the temperature set point or with carrying out
a temperature display calibration.
1. Turn the Over Temperature Limit control dial clockwise to the maximum
position indicated by the largest dot.
2. Leave the control at its maximum position until the Set the Day Mode and Night Mode
procedures have been completed, as well as the optional Temperature Display Accuracy
Verification and any resulting Calibration procedures have also been completed.
3. See page 30 for instructions on how to set the OTL after the above procedures are
completed.
25 | P a g e
Note: Terminate the auto cycle if it is running. Press and hold the Cycle Start
button for two seconds. The Program Active Light will extinguish.
1. Place the incubator into Manual Nighttime Mode
a. To switch from Daytime to Night, press and hold the Mode
button for 5 seconds. The Day Temp indicator will
extinguish, the blue Night Temp will illuminate.
Program Active Light Off
Nighttime Mode
2. Place the Temperature display into the Nighttime Set Point mode.
a. Press and release the Up or the Down arrow button.
b. The Time display will go dark.
c. The Temperature display will briefly flash the letters “SP”, then
show the blinking, adjustable Night Temp set point.
SET TEMPERATURE
SET TEMPERATURE
Initial Set Point
3. Enter a new Night set point.
a. Use the Up and Down arrow buttons.
Note: If neither button is pressed within 5 seconds, the Temperature display will
stop blinking and return to displaying the current temperature of the incubator.
SET TEMPERATURE
New Set Point
4. Wait 5 seconds after entering the new set point.
a. The Temperature display will cease flashing, and the Time
display will illuminate.
b. The Temperature display will revert to showing the Current
chamber air temperature.
c. The adjusted set point is now saved, and the incubator will
begin cooling or heating to achieve the new Nighttime set
point.
SET TEMPERATURE
Chamber Cooling to
Achieve the New Set Point
OPERATION (CONTINUED)
SET THE NIGHTTIME TEMPERATURE SET POINT
The incubator comes from the factory with a Night Mode set point of 20°C. Perform the steps below
to adjust the Night set point to that of your laboratory or study protocol, if other than 20°C.
26 | P a g e
End of procedure
Note: Terminate the auto cycle if it is running. Press and hold the Cycle Start
button for two seconds. The Program Active Light will extinguish.
1. Place the incubator into Manual Day Mode.
a. To switch from Night to Day, press and hold the Mode
button for 5 seconds. The Night Temp indicator will
extinguish, the blue Day Temp will illuminate.
Program Active Light Off
Daytime Mode
2. Place the Temperature display into the Day Set Point mode.
a. Press and release the Up or the Down arrow button.
b. The Time display will go dark.
c. The Temperature display will briefly flash the letters “SP”, then
show the blinking, adjustable Day Temp set point.
SET TEMPERATURE
SET TEMPERATURE
Initial Set Point
3. Enter a new Day set point.
a. Use the Up and Down arrow buttons.
Note: If neither key is pressed within 5 seconds, the Temperature display will stop
blinking and return to displaying the current temperature of the incubator.
SET TEMPERATURE
Adjusted Day Set Point
4. Wait 5 seconds after entering the new set point.
a. The Temperature display will cease flashing, and the Time
display will illuminate.
b. The Temperature display will revert to showing the Current
chamber air temperature.
a. The adjusted set point is now saved, and the incubator will
begin cooling or heating to achieve the new Day set point.
SET TEMPERATURE
Chamber Heating to Achieve
the New Set Point
Optional: Leave the incubator in the Day Mode if you will be performing the
optional Temperature Display Accuracy Verification procedure.
OPERATION (CONTINUED)
SET THE DAYTIME TEMPERATURE SET POINT
The incubator comes from the factory with a Day Mode set point of 20°C. Perform this procedure if
your laboratory or study protocol calls for a Day temperature other than 20°C.
End of procedure
27 | P a g e
Figure 15: Probe End 2
inches (5cm) From Shelf
Surface
Figure 14: Example of
introducing probes through
the door space
OPERATION (CONTINUED)
TEMPERATURE DISPLAY ACCURACY VERIFICATION
Note: Performing a temperature accuracy verification requires a temperature reference device.
Please see the Reference Sensor Devices entry on page 11 for the device requirements.
Optional: The unit temperature display was calibrated at the factory at 20ºC. A verification of the
display accuracy may be carried out when preparing the incubator for use if required by your
laboratory or production protocol. The verification procedure compares the displayed temperature
of the SRIPF with the actual chamber air temperature, as provided by a calibrated reference
device.
Perform the Verification procedure with the incubator operating in the manual Day Mode, as the
LED light bar introduces heat into the chamber that should be accounted for. If a difference
between the actual and displayed temperatures is discovered, perform a temperature calibration.
Please see the Calibrate Temperature Display procedure on page 40 in the User Maintenance
section.
Probes
A temperature reference device sensing probe may be introduced through
the access port. Carefully place the port stopper over the probe wires.
The probe may also be introduced through the chamber door space. Use
non-stick, non-marking tape to secure the wires to the incubator body,
and to seal any exterior gaps. The door must close and latch fully.
Place the head of the sensor probe as close as possible to the
geometric center of the incubation chamber. The probe sleeve may
be taped to the shelving using non-marking, non-stick tape, as long
as the exposed copper end is 2 inches (5cm) away from the shelf
(see Figure 15). An exposed sensor probe in direct contact with the
shelving may experience heat sinking, which can result in an
inaccurate temperature reading.
Stability
After introducing and placing the temperature probe, switch the
incubator to its manual Day Mode and allow the chamber to run
undisturbed for 8 hours, often done overnight, prior to performing
the verification. Do not change modes or launch the Day – Night
Auto Cycle during this period, as doing so risks destabilizing the
chamber air temperature.
Prior to performing the verification, the chamber must operate at
the verification temperature set point for at least 1 hour with no
fluctuations greater than ±0.1°C in order to be considered
stabilized. Failure to wait for stabilization will result in an inaccurate
verification. Do not open the door during the stabilization period or
during the verification procedure.
28 | P a g e
Procedure continued on next page
Temperature Accuracy Verification
1. Once the incubation chamber has stabilized, operating in the Manual Day
Mode, compare the reference temperature device and chamber
temperature display readings.
a. If the readings are the same, or if a difference between the two
(2) falls within the acceptable range of your protocol, the
display is accurately showing the chamber air temperature.
The Temperature Verification procedure is now complete.
b. See step 2 if a difference falls outside the acceptable range of
your protocol.
Reference Device
SET TEMPERATURE
2. If there is an unacceptable difference, a display temperature calibration
must be performed to match the display to the reference device.
a. Please see page 40 in the User Maintenance section.
Reference Device
SET TEMPERATURE
OPERATION (CONTINUED)
End of procedure
29 | P a g e
Setting the OTL Heating Cutoff
Example
1. If not already set to maximum, turn the Set Over Temperature Limit dial
all the way clockwise.
2. Increase the temperature set point to +1°C above your day application
temperature. The temperature set point will be restored to your application
temperature during step 7.
a. Briefly, press the Up Arrow button once, to place the
temperature display in its blinking, adjustable set point mode.
b. Then use the Up Arrow button to increase the temperature
set point by +1°C.
c. After entering the new set point, wait 5 seconds for the display
to exit its set point adjustment mode, automatically saving the
new set point.
Note: If neither arrow key is pressed within 5 seconds of the temperature display
being placed in the adjustable set point mode, the display will stop blinking and
return to showing the current temperature of the incubator.
SET TEMPERATURE
Entering Set Point Mode
SET TEMPERATURE
Set Point Increased
3. After increasing the set point, wait for the incubation chamber to achieve
and stabilize at the raised set point.
a. After achieving the raised set point, there should be no
temperature fluctuations of ±0.1°C or greater for 15 minutes in
order for the chamber to be considered stabilized, for this
stage of the procedure.
SET TEMPERATURE
Unit Heating to New
Set Point (21.5°C)
4. Once the chamber temperature has stabilized at the increased set point,
turn the Over Temperature Limit control dial counterclockwise until the
red Over Temperature Limit Activated light illuminates.
MAINTENANCE (CONTINUED)
SET THE OVER TEMPERATURE LIMIT
Note: Test the OTL for functionality once per year.
The incubator temperature must be stable running at your Day Mode temperature set point for at
least 1 hour prior to setting the OTL. Setting the OTL while running in the Night Mode risks tripping
the system whenever the unit is switched to a warmer Daytime temperature.
Perform the following steps to set up the Over Temperature Limit system for normal use:
30 | P a g e
Continued on next page
Setting the OTL Heating Cutoff (Continued)
5. Slowly turn the dial clockwise until the Over Temperature Limit
Activated light turns off. Stop turning the control.
a. The Over Temperature Limit is now set at approximately 1˚C
above the application temperature set point.
6. Optional: You may turn the dial slightly to the left to bracket in even
closer to the 1˚C above your application temperature set point.
7. Return the temperature set point to the application temperature of your
study or lab protocol.
a. Briefly, press the button once, to place the temperature display
in its adjustable set point mode.
b. Use down arrow key to lower the set point to your application
temperature.
SET TEMPERATURE
Application Set Point
Restored
8. Leave the OTL dial set just above the activation point.
MAINTENANCE (CONTINUED)
Notes
If the OTL activates after an extended door opening, you may turn the dial very slightly to the right
(clockwise), to deactivate the OTL, and leave the dial in that position.
If the OTL continues activating, check for ambient sources of heat or cold that may be adversely
impacting the unit temperature stability. Check if any powered accessories in the chamber are
generating heat. If you can find no sources of external or internal temperature fluctuations, contact
Tech Support or your distributor for assistance.
End of procedure
31 | P a g e
OPERATION (CONTINUED)
LOADING THE INCUBATOR
Place items on the shelves inside the incubator chamber as evenly spaced as possible. Proper
spacing allows for maximum air circulation and a high degree of temperature uniformity.
End of the Preparing the Incubator Procedure
ATTACHING EQUIPMENT TO THE INTERIOR ACCESSORY OUTLET
The incubator has a 1A (maximum) accessory outlet located inside the chamber. The power switch
on the front panel controls power to the accessory outlet. This outlet can power equipment such as
magnetic stirrers, rockers, etc. Do not attach any equipment drawing more than 1A to this outlet.
Accessory equipment may produce additional heat. This heat could affect the temperature range
and uniformity of this incubator. Verify that the incubator operates within your protocol’s required
temperature range when the accessory equipment is installed and operating.
HUMIDIFYING THE INCUBATOR
Placing only a small number of open or breathable media containers in the incubator chamber may
lead to excessive drying of sample media. Unusually dry environmental conditions may also
contribute to sample drying.
To counteract this, Sheldon Manufacturing offers an optional humidity collection pan and tubing
accessory kit: Part Number 9900708. The kit redirects moisture that normally condenses on the
heat sink fins of the Peltier TEC-H heating and cooling device, and uses it to humidify the incubator.
After ordering and receiving the kit, place the stainless steel pan on the lowest chamber surface.
Connect the tubing that comes with the kit to the port on the back of the Peltier duct cover. Run the
tubing down the back of the incubator behind the shelves, and secure the end of the tubing inside
the pan. The pan is supplied with a copper slug to help prevent microbial contamination.
The humidification kit is intended for use while running small loads.
32 | P a g e
OPERATION (CONTINUED)
MANAGING CONDENSATION
Excessive condensation in the incubation chamber may create leaks around the chamber door, and
may cause corrosion damage if allowed to continue for significant lengths of time.
Condensation takes place wherever the humidity level in the incubator chamber reaches the dew
point. The dew point is the level of humidity at which the air cannot hold more water vapor. The
warmer the air, the more water vapor it can hold. Evaporating sample media can be a source of
chamber humidity.
As the level of humidity rises in an incubation chamber, condensate will first appear on surfaces
that are cooler than the air temperature. Near the dew point, condensate forms on any item or
exposed surface that is even slightly cooler than the air. When the dew point is reached,
condensate will form on nearly all exposed surfaces.
Managing condensation primarily depends on either lowering the humidity level or increasing the air
temperature in the incubator chamber.
Note: Rising or falling air pressure from the weather will adjust the dew point up and down in small
increments. If the relative humidity in the incubation chamber is already near the dew point,
barometric fluctuations may push it across the dew point threshold.
Note: Thin air at higher altitudes holds less humidity than the denser air found at or near sea level.
If condensate is forming in the incubation chamber, wipe down and dry the chamber, then check
the following:
Make sure items on the shelves are evenly spaced to allow for good airflow.
Empty the optional accessory humidity collection pan frequently, if in use.
Are frequent or lengthy chamber door openings causing significant temperature disruptions
and chilling the chamber surfaces? If so, reduce the number of openings.
Does the ambient humidity in the room exceed the stated operating range of 80% relative
environmental humidity? If so, lower the room’s humidity.
Is the chamber door closing and latching properly? Is the door gasket leaking? Check the
gasket for damage, wear, or signs of brittleness or dryness. Replace the gasket if needed.
Is the incubator exposed to an external flow of cold air such as an air-conditioning vent or a
door to a cooler hallway or adjacent room? Block or divert the air, or move the incubator.
33 | P a g e
OPERATION (CONTINUED)
Note: In the event of a power outage, the unit will restart in the last mode it was operating in. The
incubator current time will automatically adjust to compensate. If active at the time of the
outage, the Auto Cycle will resume running and automatically advance to the Day or Night
Mode, synched with the updated current time.
LAUNCHING THE AUTO CYCLE
To start the day – night Auto Cycle carry out the following step:
1. Press and hold the Cycle Start button for two seconds.
2. The Program Active LED indicator next to the Cycle Start button will illuminate.
3. The auto cycle will now run indefinitely, switching between Day and Night Modes until
manually terminated.
ENDING THE AUTO CYCLE
To terminate the Auto Cycle perform the following step:
1. Push and hold the Cycle Start button for two seconds.
2. The Program Active indicator next to the Cycle Start button will extinguish.
34 | P a g e
OPERATION (CONTINUED)
This page left blank
35 | P a g e
Alarm OFF
1. Place the temperature display in the adjustable set point menu for either
the Daytime or Nighttime mode.
a. Press and release either the Up or the Down arrow key while
in either mode, until the screen flashes “SP”.
b. The display will then show an adjustable, flashing temperature
set point.
SET TEMPERATURE
Set Point Indicator
SET TEMPERATURE
Current Set Point, Adjustable
2. Press and hold the Up arrow until the temperature display reads “dO”.
a. “dO” indicates that “door alarm off” is selected.
SET TEMPERATURE
Door Alarm Off
3. Release the Up arrow.
a. “dO” will flash approximately six times.
b. The display will then revert to showing the current chamber
temperature.
c. The door open alarm is now set to off, and the temperature set
point has not been changed.
SET TEMPERATURE
Current Temperature
OPERATION (CONTINUED)
TURN ALARM OFF / ON
Perform the following steps to turn the door alarm off or on. Note that once set to off the alarm will
not activate until the user has turned it back on. This procedure is applicable to units manufactured
after January 15, 2015, or older units retrofitted with a new door alarm control chip.
Turn the Door Alarm Off
36 | P a g e
Procedure continued on next page
Alarm ON
1. Place the temperature display in the adjustable set point menu for either
the Daytime or Nighttime mode.
a. Press and release either the Up or the Down arrow key while
in either mode, until the screen flashes “SP”.
b. The display will then show an adjustable, flashing temperature
set point.
SET TEMPERATURE
Set Point Indicator
SET TEMPERATURE
Current Set Point, Adjustable
2. Press and hold the Up arrow until the temperature display reads “dI”.
a. “dI” indicates that “door alarm on” is selected.
SET TEMPERATURE
Door Alarm On
3. Release the up arrow.
a. “dI” will flash approximately six times.
b. The display will then revert to showing the current chamber
temperature.
c. The door alarm is now set to on, and the temperature set point
has not been changed.
SET TEMPERATURE
Current Temperature
OPERATION (CONTINUED)
Turn the Door Alarm On
End of procedure
37 | P a g e
USER MAINTENANCE
Warning: Prior to any maintenance or cleaning of this unit, disconnect the power cord from the power
supply.
Avertissement: Avant d'effectuer toute maintenance ou entretien de cet appareil, débrancher le
cordon secteur de la source d'alimentation.
CLEANING AND DISINFECTING
If a hazardous material or substance has spilled in the unit, immediately initiate your site Hazardous
Material Spill Containment protocol. Contact your local Site Safety Officer and follow instructions
per the site policy and procedures.
The unit chamber should be cleaned and disinfected prior to first use. Periodic cleaning and
disinfection are required to prevent microbiological contamination.
Do not use spray on cleaners or disinfectants. These can leak through openings and coat electrical
components. Do not use cleaners or disinfectants that contain solvents capable of harming mill
finish aluminum or paint coatings. Do not use chlorine-based bleaches or abrasives; these will
damage the chamber liner. Consult with the manufacturer or their agent if you have any doubts
about the compatibility of decontamination or cleaning agents with the parts of the equipment or
with material contained in it.
Warning: Never clean the unit with alcohol or flammable cleaners.
Avertissement: Ne jamais nettoyer l'appareil à l'alcool ou avec des nettoyants inflammables.
Cleaning
1. Remove all non-attached accessories (shelves, racks, and any additional items) from the
chamber.
2. Clean the chamber interior with a mild soap and water solution, including all corners.
3. Take special care when cleaning chamber sensor probes.
4. Clean all removable accessories and components.
5. Clean and disinfect any attached sample tubing and replace if discoloring is present.
6. Rinse the chamber surfaces and shelving with distilled water and wipe dry with a soft cloth.
Do not use deionized water.
38 | P a g e
USER MAINTENANCE (CONTINUED)
Disinfecting
Disinfect the unit chamber on a regular basis. Perform the following steps to manually disinfect the
chamber:
1. Turn the unit off. Open the door and carry out your laboratory, clinical, or production space
disinfection protocol.
2. Disinfect the unit chamber using commercially available disinfectants that are noncorrosive, non-abrasive, and suitable for use on painted surfaces. If disinfecting external
surfaces use disinfectants that will not damage painted metal or plastic. Contact your Site
Safety Officer for detailed information on the disinfectants compatible with your cultivation
or culturing applications.
3. If permitted by your protocol, remove all interior accessories (shelving and other nonattached items) from the chamber when disinfecting.
4. Disinfect all surfaces in the chamber, making sure thoroughly disinfect the corners.
Exercise care to avoid damaging the sensor probes.
5. Gas concentrations from evaporating disinfecting agents can inhibit growth or cause
metabolic symptoms in microbiological sample populations. Make sure that chlorines,
amphyls, quaternary ammonias, or any other overtly volatile disinfecting agents have been
rinsed or otherwise removed from the chamber surfaces, prior to placing samples in the
chamber.
End of procedure
MAINTAINING ATMOSPHERIC INTEGRITY
Periodically, inspect the door latch, trim, catch, and gasket for signs of deterioration. Failure to
maintain the integrity of the door system shortens the life span of the incubator.
ELECTRICAL COMPONENTS
Electrical components do not require maintenance. If the incubator fails to operate as specified,
please contact your Shel Lab Dealer or Sheldon Technical Support for assistance (please see
page 7Error! Bookmark not defined.).
39 | P a g e
Figure 17: Probe End 2
inches (5cm) From
Shelf Surface
Figure 16: Introducing a
sensor probe through the
access port.
MAINTENANCE (CONTINUED)
CALIBRATE THE TEMPERATURE DISPLAY
Note: Performing a temperature display calibration requires a temperature reference device.
Please see the Reference Sensor Devices entry on page 11 for device requirements.
Temperature calibrations are performed to match the unit temperature display to the actual air
temperature inside the incubation chamber. The actual air temperature is supplied by a calibrated
reference sensor device. Calibrations compensate for drifts in the unit microprocessor controller as
well as those caused by the natural material evolution of the sensor probe in the chamber space.
Calibrate as often as required by your laboratory or production protocol, or regulatory compliance
schedule.
Probes
A reference device sensor probe may be introduced through the access
port. Carefully place the port stopper over any probe wires (see figure 16).
Probes may also be introduced through the chamber door space. Use nonstick, non-marking tape to secure the wires to the incubator body and seal
any exterior gaps. The door must close and latch fully.
Place the head of the sensor probe as close as possible to the geometric
center of the chamber. A thermocouple sensor probe sleeve may be taped
to the shelving, as long as the exposed copper end is 2 inches (5cm) away
from the shelf (see Figure 17). An exposed sensor probe in direct contact
with the shelving may experience heat sinking, which can result in an
inaccurate temperature reading.
Stability
After introducing and placing the temperature probe, place the unit
into the manual Day Mode (Step1 on the next page). The Day Mode
temperature set point should be that of your application’s day
period. Allow the unit to operate undisturbed for at least 8 hours—
for example overnight—before starting the calibration (Step 2). This
allows the chamber temperature to stabilize.
Prior to a calibration, the chamber must operate at the Day
calibration temperature set point for at least 1 hour with no
fluctuations of ±0.1°C or greater in order to be considered
stabilized. Failure to wait for stabilization will result in an inaccurate
display calibration and reading. Do not open the door during the
stabilization period or during the calibration.
Following the day calibration, place the unit in its manual Night Mode (Step 11) and wait 2 hours.
The temperature display must be calibrated separately in the manual Day and Night Modes to
compensate for the extra heat generated during the Day Mode by the day illumination LEDs in the
chamber. Accurate calibrations cannot be carried out while the auto cycle is running.
40 | P a g e
Calibration—Day Mode
1. The incubator should already be running in Day Mode with the auto cycle
turned off during the stabilization period and calibration.
a. Turn off the auto cycle if it is running by pressing and
holding the Start Cycle button for 2 seconds.
b. If not already in the Daytime Mode, place the incubator in
Day Mode by pressing and holding the MODE button for 5
seconds.
Program Active Light Off
Day Mode
2. Allow the unit to run undisturbed, with the chamber door closed for at least
8-hours in the Day Mode to stabilize.
a. The chamber is stabilized when no temperature fluctuations of
±0.1°C or greater have been detected by the reference device
for 1 hour.
b. If the chamber door is opened at any time during the
procedure the chamber must be allowed to re-stabilize.
3. Once the temperature has stabilized, compare the reference device and
the incubator display temperature readings.
a. If the readings are the same or the difference between the two
falls within the acceptable range of your protocol, the
incubator is calibrated for Day Mode temperature.
Advance to Step 11 to start the Night Mode calibration on
page 44.
b. If a difference falls outside of your protocol range, advance to
step 4.
Reference Device
SET TEMPERATURE
4. A display calibration adjustment must be entered to match the display to
the reference device. See next step.
Reference Device
SET TEMPERATURE
MAINTENANCE (CONTINUED)
Procedure continued on next page
41 | P a g e
Daytime Temperature Calibration (Continued)
5. Place the temperature display in its calibration mode.
a. Press and hold both the UP and DOWN arrow buttons
simultaneously.
b. The Temperature Display will show the letters “CO”, then
begin flashing the current display temperature value.
Note: If an arrow key is not pressed for 5 seconds, the display will cease flashing,
and store the last displayed value as the new current chamber temperature value.
SET TEMPERATURE
Calibration Mode
SET TEMPERATURE
Current Display Value
6. Use the Up or Down arrows to adjust the current display
temperature value until it matches the reference device temperature
reading.
Reference Device
SET TEMPERATURE
Corrected Display Value
7. After correcting the display value, wait 5 seconds.
a. The Temperature Display will cease flashing, store the
correction, and resume showing the current temperature.
b. The incubator will begin heating or cooling in order to reach
Day set point with the corrected display value.
SET TEMPERATURE
Cooling to Set Point
MAINTENANCE (CONTINUED)
42 | P a g e
Procedure continued on next page
Daytime Temperature Calibration (Continued)
8. Allow the incubator to sit for one 1 hour undisturbed to stabilize after it
has achieved the set point with the corrected display.
a. Failure to wait until the incubation chamber is fully stabilized
will result in an inaccurate reading.
SET TEMPERATURE
Stabilized
9. Compare the reference device reading with the incubator temperature
display again.
a. If the reference device and the incubator temperature display readings
are the same or the difference falls within the range of your protocol,
the incubator is now calibrated for the Day Mode temperature.
Advance to Step 11 to begin the Night Mode temperature calibration.
b. See the next step if the readings fail to match or fall outside of your
protocol range.
Reference Device
SET TEMPERATURE
10. If a difference still falls outside the acceptable range of your protocol,
repeat steps 4 – 9 up to two (2) more times.
a. Three calibration attempts may be required to successfully calibrate
units that are more than ± 2°C out of calibration.
b. If the Day Mode temperature readings of the incubator and the
reference device still fall outside your protocol after three calibration
attempts, contact your distributor or Technical Support for
assistance.
Reference Device
SET TEMPERATURE
MAINTENANCE (CONTINUED)
Procedure continued on next page
43 | P a g e
Nighttime Temperature Calibration
11. Place the incubator in Night Mode.
a. Press and hold the Mode button for 5 seconds.
b. The Day Temp indicator will extinguish.
c. The blue Night Temp indicator will illuminate.
Night Mode
12. Wait 1 hour to allow the temperature to stabilize after the Temperature
display achieves the Nighttime set point.
a. The reference device temperature measurement should not
register a chamber fluctuation of ±0.1°C or greater for at least
1 hour in order for the chamber to be considered stabilized.
13. Once the temperature has stabilized, compare the reference device and
the incubator display temperature readings.
a. If the readings are the same or the difference between the two
falls within the acceptable range of your protocol, the
incubator is calibrated for Night Mode temperature. The
Calibration procedure is now finished.
b. If a difference falls outside of your protocol range, advance to
the next step.
Reference Device
SET TEMPERATURE
14. A display calibration adjustment must be entered to match the display
Night Mode to the reference device. See next step.
Reference Device
SET TEMPERATURE
MAINTENANCE (CONTINUED)
Part 2: Calibrate the Nighttime Temperature
44 | P a g e
Procedure continued on next page
Nighttime Temperature Calibration (Continued)
15. Place the unit in its temperature calibration mode.
a. Press and hold both the UP and DOWN arrow buttons
simultaneously for approximately 5 seconds.
b. The Temperature Display will show the letters “CO”, then
begin flashing the current display temperature value.
Note: If an arrow key is not pressed for five seconds, the display will cease
flashing, and store the last displayed value as the new current chamber Night
Mode temperature value
SET TEMPERATURE
Calibration Mode
SET TEMPERATURE
Current Display Value
16. Use the Up or Down arrows to adjust the current display
temperature value until it matches the reference device temperature
reading.
Reference Device
Corrected Display
17. After correcting for the offset, wait 5 seconds.
a. The incubator Temperature Display will cease flashing, store
the correction, and resume showing the current temperature.
b. The incubator will now begin heating or cooling in order to
reach your set point with the corrected display value.
SET TEMPERATURE
Cooling to Set Point
18. Allow the incubator to operate undisturbed for at least 1 hour to stabilize
after it has achieved the set point with the corrected display value.
a. Failure to wait until the incubation chamber is fully stabilized
will result in an inaccurate reading.
SET TEMPERATURE
Stabilized
MAINTENANCE (CONTINUED)
Procedure continued on next page
45 | P a g e
Nighttime Temperature Calibration (Continued)
19. Compare the reference device reading with the incubator temperature
display again.
a. If the reference device and the incubator temperature display
readings are the same or the difference falls within the range of
your protocol, the incubator is calibrated for Night Mode
temperature. The Calibration procedure is now correct.
b. See the next step if the readings still fail to match or fall outside
of your protocol range.
Reference Device
SET TEMPERATURE
20. If a difference still falls outside the acceptable range of your protocol,
repeat steps 13 – 19 up to two (2) more times.
a. Three calibration attempts may be required to successfully calibrate
units that are more than ± 2°C out of calibration.
b. If the temperature readings of the incubator and the reference device still fall
outside your protocol after three calibration attempts, contact your incubator
distributor or Technical Support for assistance.
Reference Device
SET TEMPERATURE
MAINTENANCE (CONTINUED)
End of procedure
46 | P a g e
Model
Shipping
Net Weight
SRI6PF
204lbs / 92.5kg
163lbs / 74kg
SRI20PF
363lbs / 164.7kg
295bs / 133.8kg
Model
Exterior W × D × H
Interior W × D × H
SRI6PF
30 x 31.5 x 33.5 in
25.5 x 24.0 x 18.5 in
SRI20PF
30 x 31.5 x 69.5 in
25.5 x 24.0 x 54.5 in
Model
Exterior W × D × H
Interior W × D × H
SRI6PF
76.2 x 80.01 x 85.09 cm
64.8 x 61.0 x 47.1 cm
SRI20PF
76.20 x 80.01 x 176.53 cm
64.77 x 60.96 x 138.43 cm
Model
Cubic Feet
Liter
SRI6PF
6.55
185.53
SRI20PF
19.30
546.57
UNIT SPECIFICATIONS
These incubators are 100 - 120 voltage units. Please refer to the incubator data plate to verify its
electrical specifications.
Technical data specified applies to units with standard equipment at an ambient temperature of
25°C and a voltage fluctuation of ±10%. The temperatures specified are determined in accordance
to factory standard following DIN 12880 respecting the recommended wall clearances of 10% of the
height, width, and depth of the inner chamber. All indications are average values, typical for units
produced in the series. We reserve the right to alter technical specifications at all times.
WEIGHT
DIMENSIONS
In inches
In Centimeters
CAPACITY
47 | P a g e
Model
Per Shelf
Total
SRI6PF
75lbs / 34kg
150lbs / 34kg
SRI20PF
75lbs / 34kg
375lbs / 170kg
Model
Temp Range
Uniformity
Stability
SRI6PF
15 to 40C
±0.5 @ 20C
±0.1C @ 20C
SRI20PF
15 to 40C
±0.5 @ 20C
±0.1C @ 20C
Model
Voltage
Amperage
Frequency
SRI6PF
100 – 120VAC
4.0 Amps
50/60 Hz
SRI20PF
100 – 120VAC
5.5 Amps
50/60 Hz
UNIT SPECIFICATIONS (CONTINUED)
SHELF CAPACITY BY WEIGHT
TEMPERATURE
POWER
48 | P a g e
Description
Part Number
Description
Part Number
Access Port Stopper
Humidity Reservoir Pan
and Tubing (Optional
Accessory)
Feet, Adjustable Glide
Power Cord, 125V, 15
Amp, 8 foot (2.5m) NEMA
5-15P
Fuse 6.3A
Shelf (1)
Gasket, Magnetic Door
SRI6PF (29 inches X 26
inches)
Static Shelf Mount
(1 Bracket)
Gasket, Magnetic Door
SRI20PF (29 inches X 62
inches)
Sliding Shelf Mounting Kit
(2 Brackets)
PARTS LIST
If you have the Part Number for an item, you may order the item directly from Sheldon Manufacturing by
calling (503) 646-3000 Ext. 3. If you are uncertain that you have the correct Part Number or if you need
that specific part, please contact Sheldon Technical Support for help at 1-800-322-4897 or (503) 6403000 extension 4. Please have the model number and serial number of the unit ready, as Tech Support
will need this information to match your unit with its correct part.
49 | P a g e
Loading...