Shellab SCO2W-2 User Manual

WATERJACKET CO2

INCUBATORS

WITH MICROPROCESSOR CONTROL

SCO2W SCO2W-2

PREVIOUSLY DESIGNATED

3503 3503-2

INSTALLATION AND OPERATION MANUAL

4861610

Sheldon Manufacturing Inc. P.O. Box 627 Cornelius, Oregon 97113

EMAIL: [e-mail] INTERNET: http://www.Shellab.com/~Shellab

1-800-322-4897 [phone] FAX [phone]

12/2013

2

TABLE OF CONTENTS

SECTION 1.0 RECEIVING AND INSPECTION
SECTION 2.0 GRAPHIC SYMBOLS
SECTION 3.0 INSTALLATION
SECTION 4.0 CONTROL PANEL OVERVIEW
SECTION 5.0 OPERATION
SECTION 6.0 FYRITE CO CHECKING
SECTION 7.0 HEPA C02 FILTER
SECTION 8.0 MAINTENANCE
SECTION 9.0 TROUBLESHOOTING
SECTION 10.0 PARTS LIST

UNIT SPECIFICATIONS
SCHEMATICS

These units are CO Water Jacketed incubators for professional, industrial or educational
use where the preparation or testing of materials is done at approximately atmospheric
pressure and no flammable, volatile or combustible materials are being heated. These
units are not intended for hazardous or household locations or use.

3

RECEIVING AND INSPECTION

Section

1

IMPORTANT: READ THIS INSTRUCTION MANUAL IMMEDIATELY!

Your satisfaction and safety require a complete understanding of this unit, including its
proper function and operational characteristics. Be sure operators are given adequate
training before attempting to put the unit in service. NOTE: This equipment must be used
only for its intended application; any alterations or modifications will void your warranty.

1.1 Inspection: The carrier, when accepting shipment, also accepts responsibility for safe delivery and
is liable for loss or damage claims. On delivery, inspect for visible exterior damage, note and
describe on the freight bill any damage found and enter your claim on the form supplied by the
carrier.

1.2 Inspect for concealed loss or damage on the unit itself, both interior and exterior. If any, the carrier
will arrange for official inspection to substantiate your claim. Save the shipping crate until you are
sure the unit has been delivered in good condition.

1.3 Return Shipment: If for any reason you must return the unit, contact your customer service
representative for authorization and supply nameplate data. For information on where to contact
Customer Service please see the manual cover.

1.4 Accessories: Make sure that all of the equipment indicated on the packing slip is included with the
unit. Carefully check all packaging before discarding. Each chamber comes equipped with 3
shelves, 4 shelf standards, 1 humidity pan and a power cord. All units are shipped with 4 leveling
feet and supply hose kit.

4

GRAPHIC SYMBOLS

Section

2

Your incubator has been provided with a display of graphic symbols which is designed to
help in identifying the use and function of the available user adjustable components.

2.1 Indicates that you should consult your manual for further description

or discussion of a control or user item.

2.2 Indicates "Temperature".

2.3 Indicates "Over-temperature safety".

2.4 C Indicates "Degrees Centigrade".

2.5 CO

2.6 Indicates "Gas" (CO2 for this unit).

2.7 Indicates "AC Power ".

2.8 Indicates "Water Jacket Low".

2.9 Indicates "ON/I" and "OFF/O".

2.10 Indicates "Relative Humidity".

2.11 %RH Indicates "Percent Relative Humidity".

2.12 Indicates "Potential Shock Hazard" behind this protective partition.

2.13 Indicates "Protective Earth Ground".

Indicates "Carbon Dioxide".

2

2.14 Indicates “Unit should be recycled” (Not disposed of in land-fill)

5

INSTALLATION

Section

3

Local city, county, or other ordinances may govern the use of this equipment. If you have any questions
about local requirements, please contact the appropriate local agency. Installation may be performed by the
end user.

Under normal circumstances this unit is intended for use indoors, at room temperatures between 5 and
40C, at no greater than 80% Relative Humidity (at 25) and with a supply voltage that does not vary by more

than 10%. The unit’s technical and environmental specifications includes: altitude not exceeding 2000 m

operating and Pollution Degree of 2. Customer service should be contacted for operating conditions outside
of these limits.

CAUTION: Make sure that the incubator is located in its intended position and level before filling the
water jacket. Directions for filling the jacket are in Section 5.2.

3.1 Power Source: The power supply must be properly grounded (earthed) and correctly sized to match

3.2 Location: When selecting a site for the unit, consider conditions which may affect performance,

3.3 Lifting / Handling: These units are heavy and care should be taken to use appropriate lifting

3.4 Leveling: The unit must sit level and solidly. Leveling feet are supplied and must be installed in the

3.5 Cleaning: The unit chamber should be cleaned and disinfected prior to use. Individual operating

the unit nameplate rating. The supply voltage must match the nameplate voltage within +/­10%. These units are intended for 50/60 Hz application. If supplied with a detachable cord set, plug

the female end into the inlet on the unit and the male plug into the supply. Assure that units requiring
a fuse have a fuse installed. This fuse may be at the inlet or a part of the cord set male plug.

such as heat from steam radiators, ovens, autoclaves, etc. Avoid direct sun, fast-moving air currents,
heating/cooling ducts, and high-traffic areas. To ensure air circulation around the unit, allow a
minimum of 10cm of clearance between the incubator and surrounding walls, or obstructions to free
air flow.

devices that are sufficiently rated for these loads. Units should only be lifted from their bottom
surfaces. Doors, handles and knobs are not adequate for lifting or stabilization. The unit should be
completely restrained from tipping during lifting or transport. All moving parts, such as shelves and
trays should be removed and doors need to be positively locked in the closed position during transfer
to prevent shifting and damage.

four holes in the bottom corners of the unit. With the feet installed and the unit standing upright, each
foot can be raised by turning it in a counterclockwise direction. Adjust the foot at each corner until
the unit stands level and solid without rocking. If the unit must be moved, drain all of the water from
the unit and turn the leveling feet in (clockwise) all the way to prevent damage while moving.

conditions and appropriate protocol will determine the correct procedure for decontamination. A
typical decontamination procedure that is adequate for many situations has been described below.
As well, certain steps are listed that will help reduce the likelihood of contamination and the
necessity of decontamination. Whatever process is appropriate, it needs to be done on a
regularly scheduled basis. Depending on usage and protocol, this may be monthly, quarterly or
otherwise. Regardless of the decontamination procedure used, certain precautions will need to
be taken:

A. Always disconnect the unit from the electrical service when cleaning.

Assure all volatile or flammable cleaners are evaporated and dry before reconnecting the
unit to the power supply.

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B. Special care should be taken when cleaning around sensing heads to prevent damage.
C. If you use chlorine-based bleaches or abrasive cleaners this will modify the stainless steel

interior finish. DO NOT USE hard tools such as metal wire brushes or steel wool. Use
non-abrasive cleaners and soft tools such as plastic brushes.

TYPICAL DECONTAMINATION PROCEDURE

1. Remove the humidity pan every week and autoclave, or wash with soap and water then

disinfect with 70% alcohol solution. Replace in the incubator with fresh, DISTILLED
water.

2. Flush the sample port tubing with 70% alcohol solution. Replace any lines that have a

green color.

3. Remove the door gaskets, clean and disinfect. Clean and disinfect all mounting grooves

for the door gaskets.

4. Remove all shelves, shelf supports, shelf standards and shields. Autoclave, or wash and

disinfect as described in item 1.

5. Wash and disinfect all interior surfaces.

6. Give special attention to cleaning and disinfecting all access ports, air bleeds, shaft holes,

electrical pass-through and any other passages into the chamber.

7. Replace all air and CO2 filters every six months or when noticeably dirty on the upstream

side. CO2 filters are located in the shadow box just behind the GAS IN fitting and in line
with the CO2 tubing kit. Units with flow meters require operator- supplied air pumps.
These pumps should be checked for proper air filtering.

OPERATION FOR MINIMIZING CONTAMINATION

1. Keep the outside of the incubator, including the air in the laboratory, as clean as possible.

This is particularly important for units placed directly on the floor. Do not place
incubators near doors, air vents or other areas of high air movement or traffic.

2. The floor around the unit needs to be clean. Units that are placed on the floor should be

mounted higher – typically on a caster platform – for ease of moving the unit during
cleaning and access to the back of the unit.

3. Minimize the number of times access is made to the chamber during normal operation.

4. Do not depend on the use of antibiotics to maintain uncontaminated conditions, as this is

an inadequate technique for sterilization. Preferably use aseptic techniques as described
above for maintaining sterile conditions in the incubator.

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To use your SHEL LAB CO2 Incubator USB features, you need
to download the software by clicking on the image below.

3.6 Shelves and Interior Parts: Shelving, shelf supports and a humidity pan are supplied with each
unit. See Figure 1, for proper placement of these accessories.

Figure 1

3.7 Download USB Software: Go to www.shellab.com website home page and scroll down

towards the bottom of the page. Click on this section:

Open the Excel Spreadsheet SLDL0092 and download information.

8

CONTROL PANEL OVERVIEW (See Figure 2)

Section

4

All controls are located on the front panel. Units with a detachable cord have a fused inlet located at the top
rear of the control panel. This inlet has a recessed male plug, fuse and an EMI filtering system designed to
filter out electrical interference. This inlet also prevents any internally generated interference from feeding out
to the power grid.

4.1 Power Switch: The I/O (ON/OFF) switch controls all of the power for the incubator and must be in
the I/ON position before any systems are operational. Both temperature and CO2 displays will
illuminate when the power switch is in the ON position.

4.2 Water Low Light: This pilot lamp is on whenever the internal water jacket float switch has been
tripped to the closed position. When the water drops low enough the float switch closes the circuit
turning on the indicator light.

4.3 Over Temperature Light: This pilot lamp is on whenever the Over Temperature Safety thermostat
has been activated and taken control of the element. During normal operating conditions this
indicator should never be on.

4.4 Main Temperature Control: This controller is marked in C and indicates the actual temperature
within the chamber to .1C. The UP/DOWN buttons are used for inputing the set point, calibrating
the display, and muting or unmuting the audible alarm. The HIGH and LOW alarm indicators will
light whenever there is an alarm condition associated with the temperature within the chamber. The
MUTE indicator will light whenever the audible alarm has been deactivated. Some alarm conditions
have a delay programmed in.

4.5 Heating Light: This pilot lamp is on whenever the Temperature Controller is activating the heating
element.

4.6 C02 Control On Water Jacket Incubators: The control sets the rate and the limit of injection

4.7 Injection Light: This pilot lamp is on whenever the CO2 controller is injecting CO2 into the chamber.

4.8 Over Temperature Safety Control (OTP): Located front left of the control panel, this is a hydraulic

of CO2 into the incubator based on input value and some variables. The input value is set by the
operator. This is set at 5% CO2 in production. The variables are the amount of humidity in the
chamber, and the amount of time the control has been trying to inject CO2.

At start-up, the incubator must be warmed up to operating temperature. This is set in production
at 37°C. During warm up, the water tray for humidity should be filled and placed inside. This
allows the humidity to reach 95% before CO2 is injected.

The CO2 should be turned OFF during warm up. Failure to do so will cause the control overshoot
the set value input by the operator, the injection value can be reset by setting the CO2 levels down
until the display shows “CL”. Resetting the desired value resets the injection rate and limit values
to that which was input by the operator. The unit would be then be reset to the selected
temperature on main and the door and the CO2 set to the desired level.

thermostat that is wired between the Main Temperature Controller and the heating element and
functions as an override control. If at any time the Main Temperature Control fails in the ON position
and the temperature in the chamber rises above its set point, the OTP is activated and maintains
temperature at its own set point. Note that the HEATING indicator will continue to function under the
control of the OTP.

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4.9 Interface Ports: Located on the front left of the control panel opposite the Over Temperature
Safety, these three (3) ports are where the logged data can be externally accessed through serial
and parallel functions.

4.10 CO2 Sample Port: This is located on the front left side of control panel. A sample can be drawn
to measure the CO2 content in the chamber at this port.

Figure 2

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OPERATION

Section

5

READ THIS SECTION IN ITS ENTIRETY BEFORE ATTEMPTING OPERATION!

IMPORTANT: In order to ensure the proper operation of this incubator, it is essential that
you regularly verify the accuracy of the temperature and CO2 controls. Temperature can
be confirmed by use of a thermometer inside of the chamber. CO2 can be confirmed with
the use of a Fyrite Gas Analyzer or other equipment such as a gas chromatograph. These
must be checked at least on a weekly basis. Any questions should be directed to a
technical service representative.

5.1 Power up the unit by attaching the cordset (supplied with the accessories) to the unit inlet
and the building power supply. Attach the female end of the cordset into the fused and
recessed male plug on the rear of the unit and the male plug end of the cordset into the
building supply receptacle. Turn the power switch to the I/ON position.

5.2 Filling and Draining: The front panel of the incubator has a WATER LOW warning light. Fill the
water jacket with water by means of the fill port located at the top right rear of the unit marked FILL
WJ HERE. Stop filling when the WATER LOW warning light goes off. When full, the unit holds
approximately 9.5 U.S. gallons (36.005 liters). To drain the unit, hook a suitable hose to the siphon
tube marked SIPHON HERE located to the left of the fill port. Start a siphon and allow unit to empty
into a suitable service drain or catchment area.

WATER RECOMMENDATIONS FOR USE IN WATER JACKET INCUBATORS

The addition of a dis-similar metal anode in Water Jacket incubators will allow the use of good­quality tap water to fill the Jacket. The anode is located in a threaded port near the fill port on the
rear of the unit. The anode will dissolve as it is in contact with mineral salts or dissolved gasses in
the tap water. The anode should be removed and checked for erosion every year. When the
anode appears to be over 50% eroded, a new one should be purchased form Sheldon Mfg., Inc.
(Replacement Part #0260500)

Tap water with a hardness of more than 30 parts per million (PPM) or 1.5 grains per gallon (GPG)
will require assessment of the anode on a yearly basis. Dissolved gasses should be no more than
120 PPM or 7 GPG. The Ph of the water should test to between 6.0 and 8.5. DO NOT USE
DEIONIZED WATER.

When in doubt, it is advisable to use DISTILLED WATER ONLY.

5.3 Setting the Controls: Refer to the Control Panel Overview Section 4.6 of this manual.

5.4 Calibrating the Controls:
A. Push and hold both the UP and DOWN buttons simultaneously until the control displays CP

(for temperature) or CO (for CO2) after the display will start to blink.

B. While the display is blinking the UP and DOWN buttons can be used to adjust the display to

match the actual condition in the incubator chamber.

C. If no buttons are pressed within five (5) seconds the blinking will stop and the display will

revert to showing the process or actual parameter within the incubator chamber.

5.5 Humidification: Humidification of the unit is achieved by evaporation of water from the chamber
humidity pan placed in the bottom of the incubator. By filling this stainless steel reservoir pan with
DISTILLED WATER ONLY and allowing this water supply to heat and evaporate, near saturation

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humidity is obtained. Do not use plastic, glass or other metals. Only 300 series stainless metals are
acceptable for this reservoir pan. Do not use corrosive chemicals including copper sulphate or
chlorine in the pan or chamber as damage may occur. Use Distilled Water Only. DO NOT USE
DEIONIZED WATER!

Use of disinfecting chemicals in the pan can change the surface tension of the reservoir water thus
preventing evaporation and proper humidification of the chamber. Water in the pan should be
changed and the pan cleaned at least once a week to help control contamination and to maintain
proper surface tension.

5.6 Temperature Monitoring: To insure that the incubator is operating at the desired temperature, an
accurate temperature indicator such as a certified reference thermometer should be placed in the
incubator chamber. Try to place the thermometer in the center of the chamber and spaced off the
shelf (taping the thermometer down to a petri dish cover is a method that raises it off the shelf and
prevents it from rolling and hiding the scale from view). For ease of reading the thermometer would
be better if they were electronic with a remote display so they could be read without opening the
doors.

5.7 Initial Control Settings: Set controls to the set point you want your incubator to operate at.
A. Set the OTP to its maximum position clockwise. After making initial control settings allow

the incubator to warm up and stabilize for 24 hours.

B. All units are shipped set for 37C and CO2 OFF. If other conditions are desired follow the

instructions in Section 5.3 for resetting the controls.

5.8 Temperature Calibration: It is important that the calibration be performed in the following order:
Main temperature, and then the Over Temperature Safety.

A. During main temperature calibration it is important that the inner glass door not be opened

for any reason, and that the outer heated door be opened as little as possible.

B. After the incubator has been running for 24 hours and the temperature display is stable at

the set point, read the temperature in the chamber from the reference thermometer, and if
necessary, recalibrate the display using the instructions in Section 5.4.

C. After the main temperature display has restabilized and maintained set point for several

hours, check the actual temperature again. If it does not match the display repeat the
calibration.

D. After the Main Temperatures are set and adjusted, the Overtemperature Safety Control

needs to be set. Do this by turning the OTP Control Knob counterclockwise until the
Overtemperature Light comes on (this will blink in sequence with Heating Activated Light).
Next, slowly turn the knob clockwise until the light goes off, then turn the knob another 8
degrees approximately. This should set the Overtemperature 1 degree above the Main
Temperature set point.

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5.9 CO2 Supply System and Control System: The CO2 system is rated for pressures between 15 to
20 PSI which should never be exceeded at any time. Prior to any attempt to hook up or run the CO2
control system, the water jacket must be filled and the temperatures adjusted as noted in section 5.7.

Figure 3

A. The CO2 inlet fitting is located at the back of the unit near the top (see Figure 3). It is

marked CO2 TO CHAMBER. A supply hose with in-line CO2 filter (supplied with your
accessories) connects from the fitting to a CO2 tank and regulator. When the 20 PSI tank is
empty its output pressure gauge will indicate 15 PSI. At this time the empty tank can be
replaced. Note that there are no alarms to indicate when the tank is empty, so operators
should monitor gas consumption.

B. It is highly recommended that a good quality 0-60 PSI output range DUAL STAGE pressure

regulator be used on the CO2 tank. The dual stage regulator will have two pressure gauges.
The high pressure gauge (0-4000 PSI) will indicate the pressure within the tank. The low
pressure gauge (0-60 PSI) will indicate the output pressure on the supply hose to the
incubator. Do not use single stage regulators. They will not supply a stable pressure. Just
because a regulator has two gauges does not mean that it is a dual stage regulator. Insure

that you are using a dual stage regulator.

C. It is normal for the high pressure gauge on your regulator to start out reading 800 to 1000

PSI with a full tank. The reading will drop to 500 to 800 PSI quickly and will stay there for
most of the duration of the tank. At the end of use the pressure will drop quickly to zero to
indicate that the tank is completely empty. Pure CO2 is in a liquid state in the tank, and a
constant vapor pressure is generated in the tank above the liquid level. The CO2 is drawn off
of the top as a gas. The same vapor pressure is maintained as long as any liquid is left in
the tank. When the last of the liquid has evaporated into gas then the pressure will drop
rapidly as the gas is drawn off.

D. Only medical grade CO2 should be used in your incubator. The use of any lesser grade may

damage your incubator and void your warranty.

E. The micro processor CO2 control system interprets the information from the CO2 sensor,

13

displays the CO2 concentration directly on the digital display, reads the CO2 set point and
controls the percentage of CO2 in the incubator chamber.

F. The IR controlled unit is known as a direct sensor and operates under the principal that a

certain frequency of infrared light is absorbed by CO2. The more CO2 is present the more
light is absorbed. Its accuracy is consistent no matter what the conditions are in the
incubator. The IR controller utilizes an infared spectrophotometer to directly read the flow of
CO2 gas into the chamber.

G. It is recommended that the accuracy of your CO2 control system be monitored by measuring

the actual CO2 concentration on a weekly basis with a Fyrite or other measuring device.
This should be done when the chamber has not been disturbed for an extended period of
time, i.e. after the weekend, or first thing on Monday morning.

5.10 Setting the CO2 Control: Attach the supply hose to the incubator and turn on the CO2 supply. Set
the CO2 control to the desired set point using the procedure described in section 5.3.

5.11 Adjusting CO2 Display: After the incubator has had several hours to stabilize at CO2 set point,
measure the actual CO2% with a Fyrite. If there is any difference between the Fryite and the display,
use the procedure described in section 5.4 for making the display match the Fyrite. See Section 7.0
for Fyrite use.

NOTE: When using the Fyrite, insure that gas is not being injected while the reading is being
taken. Changing the CO2 set point to 0.0 prior to taking the sample and change the set point
back to the desired value after the use of the Fyrite is finished.

5.12 Audible Alarms: The temperature control and the CO2 control are both equipped with visual and
audible alarms.

A. Temperature Alarms: The temperature control has alarm indicators for high and low

conditions that are activated whenever the actual temperature is 1 above or below the set
point.

B. CO2 Alarms: The CO2 controller has alarm indicators for high and low alarm conditions that

are activated whenever the actual CO2% is 1% above or below the set point.

C. Audible Alarms: Both controls are equipped with audible alarms that are activated when

either of the HIGH or LOW indicators are activated. The audible alarms can be muted for a
single alarm occurrence by pressing and holding down either the UP or DOWN button for
several seconds until the alarm mutes. There is a built in delay of approximately 15 minutes
on the occurrence of a LOW alarm. This time delay prevents the audible alarm from
activating every time the door is opened and the temperature and CO2 drops.

5.13 Description of nGS (NO Gas Warning) Function: (If equipped)

The CO2 Low alarm activates 15 minutes after the CO2 level drops more than 1% below the set
point. If the cause is not corrected in the next 5 minutes the CO2 display will flash nGS, indicating
a no gas warning. nGS will continue to flash until the CO2 level is within 1% of set point (or above
4% if the set point is 5%). The audible alarm can be muted but the visual low alarm indicator will
continue to be lit and the display will continue to flash nGS until the low alarm deactivates. No
action is required by the user other than to correct the cause of the CO2 drop. Note: It may take
several hours after loss of gas before the CO2 low alarm comes on. This depends on the unit’s
natural rate of decay if no CO2 is injected. CO2 low alarm does not activate until CO2 has fallen
more than 1% of set point (or below 4% if set point is 5%).

Restore gas supply. Check CO2 tank and gas supply lines to the unit.
As soon as the gas supply is restored and the CO2 level rises to less than 1% below set point

(above 4.0% if set point is 5%) the display will stop blinking nGS and will again show the sensor
reading. No action is required by the user other than correcting the cause of the drop in gas
supply. The audible alarm can be muted but the visual low alarm indicator will continue to be lit
and the display will continue to flash nGS until the low alarm deactivates.

14

FYRITE CO2 CHECKING

Section

6

Section

6

A Bacharach FYRITE CO Gas Analyzer is recommended to measure CO concentrations in the incubator
chamber. This test instrument is not supplied with the incubator but is readily available from your dealer.
Follow the instructions provided with the Fyrite instrument carefully to insure correct and accurate readings.

6.1 Press button on top of Fyrite canister to release CO concentration. Tip canister to the side to
ensure all fluid is released from the top of canister.

6.2 Loosen screw on slide scale and align top of fluid with zero on the scale. Tighten screw.

6.3 Connect hose and aspirator bulb to unit being tested. The sample port for connection is located
in the upper front corner of the right side of the incubator.

6.4 Place the hose sampling cap directly over the plunger valve on top of canister and depress firmly.

6.5 With button depressed, squeeze bulb 27 times. On the last squeeze, with bulb still deflated,
release hose from button.

6.6 Turn Fyrite canister upside down 3 times, each time allowing all fluid to flow to he opposite end of
the canister.

6.7 Tip canister to the 45 degree position to ensure all fluid has been released from top of canister.

6.8 Read CO concentration in %.

NOTE: Your Fyrite indicator will come with a complete set of detailed instructions which
should be followed carefully. The fluid used inside this Fyrite instrument is poisonous and
corrosive and must not be taken internally. In event of a spill or accidental body contact
with the Fyrite fluid, follow instructions on the refill bottle carefully.

15

HEPA CO2 FILTER

Section

7

The Hepa filter attaches to a
fixed coupling ring with a direct
“snap-in” action. Push straight
up to the coupling ring to
connect. Pull the filter straight
down to disengage.
Replacement Hepa filters are
available from Sheldon Mfg., Inc.
Part number 2800517.

The CO2 Incubator design features a HEPA filtration system with a patented copper housing. (Patent No.
6,333,004) As the chamber air is drawn through the filter system, airborne microbes and isolated
particulates are not only removed from the air, but destroyed as well. It is through copper oxidation that
the contaminants are actually killed. The HEPA filter is easily replaced without the need of any tools and
has an efficiency rating of 99.97% at 0.3 microns.

Install the HEPA Filter onto the duct prior to installing the duct in the unit.

WARNING: Ensure that when installing the duct to ensure that the duct is not touching the C02 sensor head
as this may cause erroneous readings.

16

MAINTENANCE

Section

8

NOTE: Prior to any maintenance or service on this unit, disconnect the service cord from
the power supply.

8.1 Cleaning: Cleaning and decontamination are recommended on a regular basis. To prepare the
incubator for cleaning remove all interior parts such as the shelf standards and false top. All
stainless steel parts are autoclavable. Please review the Cleaning procedures described in the
Installation section for detailed instructions.

8.2 Check CO2 supply periodically; don't let it run out. The internal auto tank switch is not alarmed.

8.3 CO2 supply lines and connections can be checked for leaks with a liquid soap solution. Apply
liquid soap solution to all areas of the supply line and observe for bubbles that will indicate a leak.

8.4 Keep the CO2 flow system free of impurities. Erratic CO2 control is usually traceable to the CO2
pressure regulator on the tank, impurities in the tank, or impurities in the solenoid valve. Replace
the CO2 in-line filters every six (6) months or when the filters have become noticeably dirty on the
upstream side. There is a CO2 filter attached to the tubing kit, and one inside the shadow box
connected to the GAS-IN line.

8.5 If the data logger fails to function as specified, check to verify that the port connections are solid
and not loose. If the data logger still fails to function properly call Customer Service for
assistance.

8.6 There is no maintenance required on any controls. If the controls fail to operate as specified,
please review Section 9.0 Troubleshooting, prior to contacting customer service.

8.7 Storage: If the incubator is to be turned off for any length of time, dry the chamber and humidity
pan thoroughly and leave at room temperature. Failure to do this may cause the interior to
become contaminated. No adjustment to controls should be required when restarting the unit. If
the unit is to be shut down for transport, drain the water jacket, remove humidity pan and shelves,

dry the chamber and disconnect the CO2 and power supply’s. See section 3.3, Lifting / Handling

for transport procedures.

17

TROUBLESHOOTING

TEMPERATURE

Temperature too high

1/ controller set too high-see section 5.3
2/ controller failed on – call Customer Service
3/ wiring error – call Customer Service

Display reads "HI" or "400"+

probe is unplugged, is broken or wire to sensor is broken – trace wire from
display to probe; move wire and watch display to see intermittent problems

Chamber temp spikes over set point
and then settles to set point

recalibrate – see section 5.4

Temperature too low

1/ OTP too low – see section 5.8.E
2/ controller set too low – see section 5.3
3/ unit not recovered from door opening – wait for display to stop changing
4/ unit not recovered from power failure or being turned off – incubators will
need 24 hours to warm up and stabilize
5/ element failure – see if heating light is on; compare current draw to data
plate
6/ controller failure – confirm with front panel lights that controller is calling
for heat
7/ OTP failure – confirm with front panel lights that it is operating correctly
8/ wiring problem – check all functions and compare wiring to diagram in
section 10.0, especially around any areas recently worked on
9/ loose connection – check shadow box for loose connections

Display reads "LO"

1/ sensor is plugged in backwards – reverse sensor wires to controller
2/ if ambient room temperature is lower than range of unit – compare set
points and ambient temperature to rated specifications in section 9.0

Unit will not heat over a temperature
that is below set point

1/ confirm that fan is moving and that amperage and voltage match data
plate – check fan motor motion in shadow box and feel for air movement in
chamber
2/ confirm that set point is set high enough ,turn OTP counterclockwise and
see if heating light or safety light comes on
3/ check connections to sensor
4/ check calibration – using independent certified reference thermometer,
follow instructions in sections 5.4 and 5.8

Unit will not heat up at all

Section

9

CAUTION: Extreme caution must be exercised any time access is made into areas housing electrical
components. Repair, replacement or adjustment of components in these areas must only be done by qualified
technicians familiar with electrical circuitry and the operation of the chamber.

Always make a visual inspection of the incubator and control console when troubleshooting. Look for loose or
disconnected wires or tubing, which may be the source of the trouble.

NOTE: IF BREAKERS TRIP REPEATEDLY, OR FUSES BLOW REPEATEDLY, CALL QUALIFIED SERVICE
PERSONNEL.

18

1/ verify that controller is asking for heat by looking for controller light – if
pilot light is not on continuously during initial start up there is a problem with
the controller
2/ check amperage – amperage should be virtually at maximum rated (data
plate) amperage
3/ do all controller functions work?
4/ is the OTP set high enough? – for diagnostics, should be fully clockwise
with the pilot light never on
5/ has the fuse/circuit breaker blown?

Indicated chamber temperature
unstable

1/ ±0.1 may be normal
2/ is fan working? – remove top panel and verify movement of cooling fan in
center of shadow box
3/ is ambient room temperature radically changing – either door opening or
room airflow from heaters or air conditioning? – stabilize ambient conditions
4/ sensor miss-located, damaged, or wires may be damaged - check
mounts for control and OTP sensors, then trace wires between sensors and
controls
5/ calibration sensitivity – call Customer Service
6/ OTP set too low – be sure that OTP set point is more than 5 degrees over
desired Main set point; check if OTP pilot is on continuously; turn controller
knob completely clockwise to see if problem solved then follow instructions in
section 5.8.E for correct setting.
7/ electrical noise – remove nearby sources of RFI including motors, arcing
relays or radio transmitters
8/ bad connection on temperature sensor or faulty sensor – check
connectors for continuity and mechanical soundness while watching display
for erratic behavior; check sensor and wiring for mechanical damage
9/ bad connections or faulty capacitor – check connectors for mechanical
soundness and look for corrosion around terminals or signs of arcing or other
visible deterioration

Will not maintain set point

1/ assure that set point is at least 5 degrees over ambient room temperature
2/ see if ambient is fluctuating

Display and reference thermometer
don’t match

1/ calibration error – see section 5.4
2/ temperature sensor failure – evaluate if pilot light is operating correctly
3/ controller failure – evaluate if pilot light is operating correctly
4/ allow at least two hours to stabilize
5/ verify that reference thermometer is certified

Can't adjust set points or calibration

1/ turn entire unit off and on to reset
2/ if repeatedly happens, call Customer Service

Calibrated at one temperature, but not
at another

This can be a normal condition when operating temperature varies widely.
For maximum accuracy, calibration should be done at or as close to the set
point temperature.

CO2 LEVEL

Overshoots set point but stabilizes ­display and Fyrite match

19

1/ turn set point up and down to see if solenoid valve works by feeling
and listening to valve
2/ recalibrate with Fyrite, see section 5.11 and section 7.0
3/ fan not operating correctly
a- fan motor stopped
b- fan blade fell off
c- wrong fan blade installed or mounted backwards
4/ wrong restrictor installed or missing altogether
5/ tank pressure too high, see section 5.9.A
6/ CO2 sensor partially plugged with dirt or condensation
7/ regulator set wrong, see section 5.9
8/ incubator too heavily loaded
9/ incubator being operated without shelving
10/ CO2 attached to sample port instead of “CO2 IN” fitting
12/ if display and Fyrite does not match, see Troubleshooting Co2-5

Overshoots set point and continues to rise

- display and Fyrite match

1/ debris in solenoid causing it to leak continuously
2/ solenoid failed while open
3/ unit miss-wired
4/ controller output failed or shorted
5/ CO2 sensor or interface failure
6/ CO2 sensor plugged by debris or condensation

Rises very slowly

1/ restrictor partially plugged
2/ filter overly dirty or partially plugged
3/ CO2 tank regulator set too low, see section 5.9
4/ hose kinked or leaking
5/ poor door seal
6/ CO2 tank contains mixed gas, not 100% medical grade CO2

Never rises

1/ CO2 tank empty
2/ solenoid failed while closed
3/ CO2 controller output failed while open
4/ restrictor plugged
5/ CO2 hose blockage
6/ CO2 filter plugged
7/ set point is at 0.0 and has not been reset, see section 5.3 and 5.4

Display and Fyrite reading do not match

1/ calibration error - clear chamber for 12 hrs and confirm at "0"
2/ turn set point up and down to see if solenoid valve works by feeling
and listening to valve
3/ assure that IR tube block is completely tight, 24 series
4/ if display is varying when doing Fyrite test, have a leak on IR base or
mounting plate, or CO2 is injecting at time of test.
5/ take voltage reading across sensor leads 0=0V and 20=1V
6/ assure that sensor is properly mounted
7/ DISPLAY LOWER THAN FYRITE: air leak around the CO2 sensor or
the fan motor shaft
8/ DISPLAY HIGHER THAN FYRITE: leak in tube from sample port to
chamber, verify with liquid soap solution.
9/ DISPLAY HIGHER THAN FYRITE: Fyrite fluid worn out, replace fluid
with Fyrite refill.
10/ DISPLAY HIGHER THAN FYRITE: poor Fyrite sampling technique,
see section 6.0
11/ DISPLAY HIGHER THAN FYRITE: defective Fyrite pump or tubing
12/ CO2 sensor, interface or controller failure

Is unstable – display or actual reading
varies around set point

20

1/ confirm that fan is working
2/ check for air leak around CO2 sensor or fan motor shaft
3/ magnetic field interference
4/ door not sealing properly
5/ atmospheric pressure fluctuations
6/ defective Fyrite pump or tubing
7/ CO2 entering chamber too quickly
a- tank pressure too high
b- regulator set wrong
c- restrictor incorrect or missing
8/ electronic problem with CO2 sensor, interface or controller
9/ top of unit exposed to cold air drafts
10/ unit being operated without shadow box cover
11/ electrical interference – atmospheric or conducted
12/ incubator too heavily loaded

Can't adjust set points or calibration ­"locked up"

1/ turn unit off and on to reset processor in controller
2/ if repeatedly happens, call Customer Service

Feeding continuously or abnormally high
CO2 usage

1/ do decay test: if more than 1% decay in an hour, check for leak : door
gasket tightness, motor inlet to chamber, sensor or probe inlet to
chamber, chamber access tube plugged
2/ check output signal from controller
3/ check solenoid valve for correct operation
4/ leak in plumbing including between regulator and CO2 tank
5/ door being opened too often

Won’t hold calibration – Fyrite reading
varies but display stable

1/ atmospheric pressure fluctuations
2/ top of unit exposed to cold air drafts
3/ unit being operated without shadow box cover in place
4/ condensation collecting on CO2 sensor
5/ CO2 sensor or interface failure
6/ unit incorrectly calibrated, see section 5.4
7/ taking Fyrite reading too soon after the door has been opened
8/ air leak around CO2 sensor mounting plate

Low CO2 alarm activated and nGS (NO
Gas warning) flashing on CO2 display.

CO2 gas supply has been interrupted or run out.
Tank ran out of gas or did not switch over.
Pinched gas line tubing
Severe leak in gas line
Tubing is disconnected from the gas supply..

Low CO2 alarm activated and nGS (NO
Gas warning) flashing on CO2 display.

CO2 gas supply has been interrupted or run out.
Tank ran out of gas or did not switch over.
Pinched gas line tubing
Severe leak in gas line
Tubing is disconnected from the gas supply.

MECHANICAL

Door not sealing

1/ check physical condition of gasket
2/ confirm that door latch pulls door in tightly
3/ assure that gasket is in original location

Motor doesn't move

21

1/ if shaft spins freely: check connections to motor and check voltage to
motor;
2/ if shaft rubs or is frozen, relieve binding and retest

Motor makes noise

1) Make sure that the fan or blower wheel is not contacting its housing.
Adjust the motor mounting bracket position to re-center the fan or blower
wheel, if necessary.

2) Check the fan or blower wheel for damage or out of balance condition.
Replace the fan or blower wheel if it is damaged or out of balance.

3) Turn the motor shaft to make sure that it spins freely. If it binds or the
bearings make a rubbing or scrapping sound then replace the motor.

Solenoid valve buzzing

After removing solenoid clean with alcohol for carbon build-up then blow
out. Check valve seat or channels for contamination. Check CO2 filter
and/or grade of CO2 used. If seat is worn, replace.

OTHER

Controller on at all times - "locked-up"

1/ turn unit off and on to reset
2/ if cannot change any condition on the front panel, call Customer
Service

Front panel displays are all off

1/ Check for wire damage.

Unit or wall fuse/circuit breaker is blown

1/ check wall power source
2/ compare current draw and compare to specs on data plate
3/ see what other loads are on the wall circuit

Unit will not turn on

1/ check wall power source
2/ check fuse/circuit breaker on unit or in wall
3/ see if unit is on, e.g., fan or heater, and just controller is off
4/ check all wiring connections, esp. around the on/off switch

Unit is smoking – Out of box

Put unit under vent and run at full power for one hour.

Contamination in chamber

1/ see cleaning procedure in Installation section 3.0
2/ develop and follow Standard operating procedure for specific
application; include definition of cleaning technique and maintenance
schedule.

NOTE: THE TEMPERATURE OF THE GLASS DOOR AND THE INTERIOR CHAMBER WALLS MUST BE EQUAL
TO PREVENT CONDENSATION.

The incubator is designed so that no internal electrical servicing should be required under normal conditions. If
electrical servicing is necessary, it should be performed by qualified service personnel. FOR PERSONAL SAFETY,
ALWAYS DISCONNECT THE POWER BEFORE SERVICING.

22

PARTS LIST

Description

SCO2W

115V

SCO2W-2

220V

Anode

0260500

0260500

Cable Panel Mount USB Type B Extention

1800533

1800533

CO2 Display Board

1750735

1750735

CO2 Filter

2800525

2800525

CO2 Pre-Heater

2350546

2350551

CO2 Sensor w/ Interface

9700501

9700501

Cord Set

1800510

1800539

Cord Set - European

NA

1800500

Door Heater

2350550

2350550

Door Switch

7850578

7850578

Element Coil

2350503

2350554

Fan Blade

2600543

2600543

Fan Motor

4880508

4880507

Glass Door Latch

9530516

9530516

Gasket

3450645

3450645

Humidity Pan

995-00015

995-00015

IR Transmitter

8320510

8320510

Leveling Feet

2700512

2700512

Overtemperature Safety

1750860

1750860

Pod Control 4 Loop

1750725

1750725

Power Inlet Fuse

3300516

3300515

Power Inlet

4200522

5200522

Power Switch

7850532

7850532

Shelf

5071131

5071131

SL Logo Slug

5800529

5800529

Solenoid Valve

8600528

8600529

Temperature Display Board

1750734

1750734

USB to RS232 Bridge Board

1750846

1750846

Water Level Switch

7850575

7850575

Section

10

23

UNIT SPECIFICATIONS

Weight

Shipping

Net

SCO2W SCO2W-2

(3503 3503-2)

175 lbs.

140 lbs.

Dimensions

Exterior WxDxH (IN.)

Interior

WxDxH (IN.)

SCO2W SCO2W-2

(3503 3503-2)

20x 21.6 x 26.5

15.8 x 15.8 x 12.5

Capacity

Cubic Feet

SCO2W SCO2W-2

(3503 3503-2)

1.8

Temperature

Range

Uniformity

CO2

SCO2W SCO2W-2

(3503 3503-2

Amb. 8C to 60C

+0.2C @ 37C

0 – 20%

Model

Amp

Voltage

Hertz

SCO2W (3503)

5 amp

110-120V

50/60 Hz

SCO2W-2 (3503-2)

3.5 amp

208-240V

50/60 Hz

24

FIGURE 5

M

GREEN CHERRY

ROUND

POWER SWITCH

SL# 7850532

POWER INLET
6A SL# 104179

WATER FILL

SWITCH SL# 210004

WATER FILL INDICATOR

SL# 200020

CO2 PREHEATER SL# 100909

6W

HEATING INDICATOR SL# 200021

OTP SL# 100001J

OTP INDICATOR

SL# 200020

MAIN HEATING ELEMENT

SL# 2350503

DOOR HEATERS

SL# 103068

10W

10W
10W

10W

CO2 INDICATOR

SL# 200021

CO2 SOLENOID

SL# 8600528

CIRCULATING FAN

MOTOR SL# 4880508

4 LOOP CONTROL

BOARD SL# 1750730

J8

MAIN TEMP

SENSOR

DOOR

SENSOR

J9

CO2

SENSOR

J17

HUMIDITY
SENSOR

J16

BUSS

LOAD 1

LOAD 2

LOAD 3

LOAD 4

J1

J2

J3

J4

J5

J6

J7

EARTH GRD

(NEUT)

(HOT)

J10

COMMUNICATION

ALARM CONTACTS

IR CO2 SENSOR

SL# 8320510

12VDC

POWER SUPPLY

SL# 6750507

DOOR SWITCH

SL# X1000022

SL LOGO

LIGHT BOARD

SL# 1750737

ALARM JACK

SL# 100163

HOT TERMINAL BLOCK

NEUTRAL TERMINAL BLOCK

TEMP

DISPLAY

SL# 1750734

CO2

DISPLAY

SL# 1750735

USB BOARD
SL# 1750846

USB EXSTENSION

CABLE SL# 1800533

.47uf

BLACK

BLACK

BLACK

BLACK

WHITE

WHITE

WHITE

WHITE

WHITE

WHITE

WHITE

WHITE

WHITE WHITE

WHITE

WHITEWHITE

ORANGE

ORANGE

YELLOW

YELLOW

RED

BLUE

BLACK

BLACK

WHITE

RED

BLACK

WH HT

BL HT

WHITE

BLACK

WIRING DIAGRAM

SCO2W (3503) 110V

25

WIRING DIAGRAM

M

GREEN CHERRY

ROUND

POWER SWITCH

SL# 7850532

FUSED POWER INLET

6A SL# 104179

WATER FILL

SWITCH SL# 210004

WATER FILL INDICATOR

SL# 200020

CO2 PREHEATER SL# 103328

6W

HEATING INDICATOR SL# 200021

OTP SL# 100001J

OTP INDICATOR

SL# 200020

MAIN HEATING ELEMENT

SL# 120071

DOOR HEATERS

SL# 103068

10W
10W
10W

10W

CO2 INDICATOR

SL# 200021

CO2 SOLENOID

SL# 8600529

CIRCULATING FAN

MOTOR SL# 4880507

4 LOOP CONTROL

BOARD SL# 1750730

J8

MAIN TEMP

SENSOR

DOOR

SENSOR

J9

CO2

SENSOR

J17

HUMIDITY

SENSOR

J16

BUSS

LOAD 1

LOAD 2

LOAD 3

LOAD 4

J1

J2

J3

J4

J5

J6

J7

EARTH GRD

(NEUT)

(HOT)

J10

COMMUNICATION

ALARM CONTACTS

IR CO2 SENSOR

SL# 8320510

12VDC

POWER SUPPLY

SL# 6750507

DOOR SWITCH

SL# X1000022

SL LOGO

LIGHT BOARD

SL# 1750737

ALARM JACK

SL# 100163

HOT TERMINAL BLOCK

NEUTRAL TERMINAL BLOCK

TEMP

DISPLAY

SL# 1750734

CO2

DISPLAY

SL# 1750735

USB BOARD
SL# 1750846

USB EXSTENSION

CABLE SL# 1800533

.47uf

BLACK

BLACK

BLACK

BLACK

WHITE

WHITE

WHITE

WHITE

WHITE

WHITE

WHITE

WHITE

WHITE WHITE

WHITE

WHITEWHITE

ORANGE

ORANGE

YELLOW

YELLOW

RED

BLUE

BLACK

BLACK

WHITE

RED

BLACK

WH HT

BL HT

WHITE

BLACK

WHITE

FUSE HOLDER

SL# 3300501

SCO2W-2 (3503-2) 220V