Shellab SCO2W-2 User Manual

WATERJACKET CO2
INCUBATORS
WITH MICROPROCESSOR CONTROL
SCO2W SCO2W-2
PREVIOUSLY DESIGNATED
INSTALLATION AND OPERATION MANUAL
4861610
Sheldon Manufacturing Inc. P.O. Box 627 Cornelius, Oregon 97113
EMAIL: tech@Shellab.com INTERNET: http://www.Shellab.com/~Shellab
1-800-322-4897 (503) 640-3000 FAX (503) 640-1366
12/2013
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TABLE OF CONTENTS
SECTION 1.0 RECEIVING AND INSPECTION SECTION 2.0 GRAPHIC SYMBOLS SECTION 3.0 INSTALLATION SECTION 4.0 CONTROL PANEL OVERVIEW SECTION 5.0 OPERATION SECTION 6.0 FYRITE CO CHECKING SECTION 7.0 HEPA C02 FILTER SECTION 8.0 MAINTENANCE SECTION 9.0 TROUBLESHOOTING SECTION 10.0 PARTS LIST
UNIT SPECIFICATIONS SCHEMATICS
These units are CO Water Jacketed incubators for professional, industrial or educational use where the preparation or testing of materials is done at approximately atmospheric pressure and no flammable, volatile or combustible materials are being heated. These units are not intended for hazardous or household locations or use.
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RECEIVING AND INSPECTION
Section
1
IMPORTANT: READ THIS INSTRUCTION MANUAL IMMEDIATELY!
Your satisfaction and safety require a complete understanding of this unit, including its proper function and operational characteristics. Be sure operators are given adequate training before attempting to put the unit in service. NOTE: This equipment must be used only for its intended application; any alterations or modifications will void your warranty.
1.1 Inspection: The carrier, when accepting shipment, also accepts responsibility for safe delivery and is liable for loss or damage claims. On delivery, inspect for visible exterior damage, note and describe on the freight bill any damage found and enter your claim on the form supplied by the carrier.
1.2 Inspect for concealed loss or damage on the unit itself, both interior and exterior. If any, the carrier will arrange for official inspection to substantiate your claim. Save the shipping crate until you are sure the unit has been delivered in good condition.
1.3 Return Shipment: If for any reason you must return the unit, contact your customer service representative for authorization and supply nameplate data. For information on where to contact Customer Service please see the manual cover.
1.4 Accessories: Make sure that all of the equipment indicated on the packing slip is included with the unit. Carefully check all packaging before discarding. Each chamber comes equipped with 3 shelves, 4 shelf standards, 1 humidity pan and a power cord. All units are shipped with 4 leveling feet and supply hose kit.
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GRAPHIC SYMBOLS
Section
2
Your incubator has been provided with a display of graphic symbols which is designed to help in identifying the use and function of the available user adjustable components.
2.1 Indicates that you should consult your manual for further description
or discussion of a control or user item.
2.2 Indicates "Temperature".
2.3 Indicates "Over-temperature safety".
2.4 C Indicates "Degrees Centigrade".
2.5 CO
2.6 Indicates "Gas" (CO2 for this unit).
2.7 Indicates "AC Power ".
2.8 Indicates "Water Jacket Low".
2.9 Indicates "ON/I" and "OFF/O".
2.10 Indicates "Relative Humidity".
2.11 %RH Indicates "Percent Relative Humidity".
2.12 Indicates "Potential Shock Hazard" behind this protective partition.
2.13 Indicates "Protective Earth Ground".
Indicates "Carbon Dioxide".
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2.14 Indicates “Unit should be recycled” (Not disposed of in land-fill)
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INSTALLATION
Section
3
Local city, county, or other ordinances may govern the use of this equipment. If you have any questions about local requirements, please contact the appropriate local agency. Installation may be performed by the end user.
Under normal circumstances this unit is intended for use indoors, at room temperatures between 5 and 40C, at no greater than 80% Relative Humidity (at 25) and with a supply voltage that does not vary by more
than 10%. The unit’s technical and environmental specifications includes: altitude not exceeding 2000 m
operating and Pollution Degree of 2. Customer service should be contacted for operating conditions outside of these limits.
CAUTION: Make sure that the incubator is located in its intended position and level before filling the water jacket. Directions for filling the jacket are in Section 5.2.
3.1 Power Source: The power supply must be properly grounded (earthed) and correctly sized to match
3.2 Location: When selecting a site for the unit, consider conditions which may affect performance,
3.3 Lifting / Handling: These units are heavy and care should be taken to use appropriate lifting
3.4 Leveling: The unit must sit level and solidly. Leveling feet are supplied and must be installed in the
3.5 Cleaning: The unit chamber should be cleaned and disinfected prior to use. Individual operating
the unit nameplate rating. The supply voltage must match the nameplate voltage within +/­10%. These units are intended for 50/60 Hz application. If supplied with a detachable cord set, plug
the female end into the inlet on the unit and the male plug into the supply. Assure that units requiring a fuse have a fuse installed. This fuse may be at the inlet or a part of the cord set male plug.
such as heat from steam radiators, ovens, autoclaves, etc. Avoid direct sun, fast-moving air currents, heating/cooling ducts, and high-traffic areas. To ensure air circulation around the unit, allow a minimum of 10cm of clearance between the incubator and surrounding walls, or obstructions to free air flow.
devices that are sufficiently rated for these loads. Units should only be lifted from their bottom surfaces. Doors, handles and knobs are not adequate for lifting or stabilization. The unit should be completely restrained from tipping during lifting or transport. All moving parts, such as shelves and trays should be removed and doors need to be positively locked in the closed position during transfer to prevent shifting and damage.
four holes in the bottom corners of the unit. With the feet installed and the unit standing upright, each foot can be raised by turning it in a counterclockwise direction. Adjust the foot at each corner until the unit stands level and solid without rocking. If the unit must be moved, drain all of the water from the unit and turn the leveling feet in (clockwise) all the way to prevent damage while moving.
conditions and appropriate protocol will determine the correct procedure for decontamination. A typical decontamination procedure that is adequate for many situations has been described below. As well, certain steps are listed that will help reduce the likelihood of contamination and the necessity of decontamination. Whatever process is appropriate, it needs to be done on a regularly scheduled basis. Depending on usage and protocol, this may be monthly, quarterly or otherwise. Regardless of the decontamination procedure used, certain precautions will need to be taken:
A. Always disconnect the unit from the electrical service when cleaning.
Assure all volatile or flammable cleaners are evaporated and dry before reconnecting the unit to the power supply.
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B. Special care should be taken when cleaning around sensing heads to prevent damage. C. If you use chlorine-based bleaches or abrasive cleaners this will modify the stainless steel
interior finish. DO NOT USE hard tools such as metal wire brushes or steel wool. Use non-abrasive cleaners and soft tools such as plastic brushes.
TYPICAL DECONTAMINATION PROCEDURE
1. Remove the humidity pan every week and autoclave, or wash with soap and water then
disinfect with 70% alcohol solution. Replace in the incubator with fresh, DISTILLED water.
2. Flush the sample port tubing with 70% alcohol solution. Replace any lines that have a
green color.
3. Remove the door gaskets, clean and disinfect. Clean and disinfect all mounting grooves
for the door gaskets.
4. Remove all shelves, shelf supports, shelf standards and shields. Autoclave, or wash and
disinfect as described in item 1.
5. Wash and disinfect all interior surfaces.
6. Give special attention to cleaning and disinfecting all access ports, air bleeds, shaft holes,
electrical pass-through and any other passages into the chamber.
7. Replace all air and CO2 filters every six months or when noticeably dirty on the upstream
side. CO2 filters are located in the shadow box just behind the GAS IN fitting and in line with the CO2 tubing kit. Units with flow meters require operator- supplied air pumps. These pumps should be checked for proper air filtering.
OPERATION FOR MINIMIZING CONTAMINATION
1. Keep the outside of the incubator, including the air in the laboratory, as clean as possible.
This is particularly important for units placed directly on the floor. Do not place incubators near doors, air vents or other areas of high air movement or traffic.
2. The floor around the unit needs to be clean. Units that are placed on the floor should be
mounted higher – typically on a caster platform – for ease of moving the unit during cleaning and access to the back of the unit.
3. Minimize the number of times access is made to the chamber during normal operation.
4. Do not depend on the use of antibiotics to maintain uncontaminated conditions, as this is
an inadequate technique for sterilization. Preferably use aseptic techniques as described above for maintaining sterile conditions in the incubator.
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To use your SHEL LAB CO2 Incubator USB features, you need to download the software by clicking on the image below.
3.6 Shelves and Interior Parts: Shelving, shelf supports and a humidity pan are supplied with each unit. See Figure 1, for proper placement of these accessories.
Figure 1
3.7 Download USB Software: Go to www.shellab.com website home page and scroll down
towards the bottom of the page. Click on this section:
Open the Excel Spreadsheet SLDL0092 and download information.
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CONTROL PANEL OVERVIEW (See Figure 2)
Section
4
All controls are located on the front panel. Units with a detachable cord have a fused inlet located at the top rear of the control panel. This inlet has a recessed male plug, fuse and an EMI filtering system designed to filter out electrical interference. This inlet also prevents any internally generated interference from feeding out to the power grid.
4.1 Power Switch: The I/O (ON/OFF) switch controls all of the power for the incubator and must be in the I/ON position before any systems are operational. Both temperature and CO2 displays will illuminate when the power switch is in the ON position.
4.2 Water Low Light: This pilot lamp is on whenever the internal water jacket float switch has been tripped to the closed position. When the water drops low enough the float switch closes the circuit turning on the indicator light.
4.3 Over Temperature Light: This pilot lamp is on whenever the Over Temperature Safety thermostat has been activated and taken control of the element. During normal operating conditions this indicator should never be on.
4.4 Main Temperature Control: This controller is marked in C and indicates the actual temperature within the chamber to .1C. The UP/DOWN buttons are used for inputing the set point, calibrating the display, and muting or unmuting the audible alarm. The HIGH and LOW alarm indicators will light whenever there is an alarm condition associated with the temperature within the chamber. The MUTE indicator will light whenever the audible alarm has been deactivated. Some alarm conditions have a delay programmed in.
4.5 Heating Light: This pilot lamp is on whenever the Temperature Controller is activating the heating element.
4.6 C02 Control On Water Jacket Incubators: The control sets the rate and the limit of injection
4.7 Injection Light: This pilot lamp is on whenever the CO2 controller is injecting CO2 into the chamber.
4.8 Over Temperature Safety Control (OTP): Located front left of the control panel, this is a hydraulic
of CO2 into the incubator based on input value and some variables. The input value is set by the operator. This is set at 5% CO2 in production. The variables are the amount of humidity in the chamber, and the amount of time the control has been trying to inject CO2.
At start-up, the incubator must be warmed up to operating temperature. This is set in production at 37°C. During warm up, the water tray for humidity should be filled and placed inside. This allows the humidity to reach 95% before CO2 is injected.
The CO2 should be turned OFF during warm up. Failure to do so will cause the control overshoot the set value input by the operator, the injection value can be reset by setting the CO2 levels down until the display shows “CL”. Resetting the desired value resets the injection rate and limit values to that which was input by the operator. The unit would be then be reset to the selected temperature on main and the door and the CO2 set to the desired level.
thermostat that is wired between the Main Temperature Controller and the heating element and functions as an override control. If at any time the Main Temperature Control fails in the ON position and the temperature in the chamber rises above its set point, the OTP is activated and maintains temperature at its own set point. Note that the HEATING indicator will continue to function under the control of the OTP.
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4.9 Interface Ports: Located on the front left of the control panel opposite the Over Temperature Safety, these three (3) ports are where the logged data can be externally accessed through serial and parallel functions.
4.10 CO2 Sample Port: This is located on the front left side of control panel. A sample can be drawn to measure the CO2 content in the chamber at this port.
Figure 2
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OPERATION
Section
5
READ THIS SECTION IN ITS ENTIRETY BEFORE ATTEMPTING OPERATION!
IMPORTANT: In order to ensure the proper operation of this incubator, it is essential that you regularly verify the accuracy of the temperature and CO2 controls. Temperature can be confirmed by use of a thermometer inside of the chamber. CO2 can be confirmed with the use of a Fyrite Gas Analyzer or other equipment such as a gas chromatograph. These must be checked at least on a weekly basis. Any questions should be directed to a technical service representative.
5.1 Power up the unit by attaching the cordset (supplied with the accessories) to the unit inlet and the building power supply. Attach the female end of the cordset into the fused and recessed male plug on the rear of the unit and the male plug end of the cordset into the building supply receptacle. Turn the power switch to the I/ON position.
5.2 Filling and Draining: The front panel of the incubator has a WATER LOW warning light. Fill the water jacket with water by means of the fill port located at the top right rear of the unit marked FILL WJ HERE. Stop filling when the WATER LOW warning light goes off. When full, the unit holds approximately 9.5 U.S. gallons (36.005 liters). To drain the unit, hook a suitable hose to the siphon tube marked SIPHON HERE located to the left of the fill port. Start a siphon and allow unit to empty into a suitable service drain or catchment area.
WATER RECOMMENDATIONS FOR USE IN WATER JACKET INCUBATORS
The addition of a dis-similar metal anode in Water Jacket incubators will allow the use of good­quality tap water to fill the Jacket. The anode is located in a threaded port near the fill port on the rear of the unit. The anode will dissolve as it is in contact with mineral salts or dissolved gasses in the tap water. The anode should be removed and checked for erosion every year. When the anode appears to be over 50% eroded, a new one should be purchased form Sheldon Mfg., Inc. (Replacement Part #0260500)
Tap water with a hardness of more than 30 parts per million (PPM) or 1.5 grains per gallon (GPG) will require assessment of the anode on a yearly basis. Dissolved gasses should be no more than 120 PPM or 7 GPG. The Ph of the water should test to between 6.0 and 8.5. DO NOT USE DEIONIZED WATER.
When in doubt, it is advisable to use DISTILLED WATER ONLY.
5.3 Setting the Controls: Refer to the Control Panel Overview Section 4.6 of this manual.
5.4 Calibrating the Controls: A. Push and hold both the UP and DOWN buttons simultaneously until the control displays CP
(for temperature) or CO (for CO2) after the display will start to blink.
B. While the display is blinking the UP and DOWN buttons can be used to adjust the display to
match the actual condition in the incubator chamber.
C. If no buttons are pressed within five (5) seconds the blinking will stop and the display will
revert to showing the process or actual parameter within the incubator chamber.
5.5 Humidification: Humidification of the unit is achieved by evaporation of water from the chamber humidity pan placed in the bottom of the incubator. By filling this stainless steel reservoir pan with DISTILLED WATER ONLY and allowing this water supply to heat and evaporate, near saturation
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humidity is obtained. Do not use plastic, glass or other metals. Only 300 series stainless metals are acceptable for this reservoir pan. Do not use corrosive chemicals including copper sulphate or chlorine in the pan or chamber as damage may occur. Use Distilled Water Only. DO NOT USE DEIONIZED WATER!
Use of disinfecting chemicals in the pan can change the surface tension of the reservoir water thus preventing evaporation and proper humidification of the chamber. Water in the pan should be changed and the pan cleaned at least once a week to help control contamination and to maintain proper surface tension.
5.6 Temperature Monitoring: To insure that the incubator is operating at the desired temperature, an accurate temperature indicator such as a certified reference thermometer should be placed in the incubator chamber. Try to place the thermometer in the center of the chamber and spaced off the shelf (taping the thermometer down to a petri dish cover is a method that raises it off the shelf and prevents it from rolling and hiding the scale from view). For ease of reading the thermometer would be better if they were electronic with a remote display so they could be read without opening the doors.
5.7 Initial Control Settings: Set controls to the set point you want your incubator to operate at. A. Set the OTP to its maximum position clockwise. After making initial control settings allow
the incubator to warm up and stabilize for 24 hours.
B. All units are shipped set for 37C and CO2 OFF. If other conditions are desired follow the
instructions in Section 5.3 for resetting the controls.
5.8 Temperature Calibration: It is important that the calibration be performed in the following order: Main temperature, and then the Over Temperature Safety.
A. During main temperature calibration it is important that the inner glass door not be opened
for any reason, and that the outer heated door be opened as little as possible.
B. After the incubator has been running for 24 hours and the temperature display is stable at
the set point, read the temperature in the chamber from the reference thermometer, and if necessary, recalibrate the display using the instructions in Section 5.4.
C. After the main temperature display has restabilized and maintained set point for several
hours, check the actual temperature again. If it does not match the display repeat the calibration.
D. After the Main Temperatures are set and adjusted, the Overtemperature Safety Control
needs to be set. Do this by turning the OTP Control Knob counterclockwise until the Overtemperature Light comes on (this will blink in sequence with Heating Activated Light). Next, slowly turn the knob clockwise until the light goes off, then turn the knob another 8 degrees approximately. This should set the Overtemperature 1 degree above the Main Temperature set point.
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5.9 CO2 Supply System and Control System: The CO2 system is rated for pressures between 15 to 20 PSI which should never be exceeded at any time. Prior to any attempt to hook up or run the CO2 control system, the water jacket must be filled and the temperatures adjusted as noted in section 5.7.
Figure 3
A. The CO2 inlet fitting is located at the back of the unit near the top (see Figure 3). It is
marked CO2 TO CHAMBER. A supply hose with in-line CO2 filter (supplied with your accessories) connects from the fitting to a CO2 tank and regulator. When the 20 PSI tank is empty its output pressure gauge will indicate 15 PSI. At this time the empty tank can be replaced. Note that there are no alarms to indicate when the tank is empty, so operators should monitor gas consumption.
B. It is highly recommended that a good quality 0-60 PSI output range DUAL STAGE pressure
regulator be used on the CO2 tank. The dual stage regulator will have two pressure gauges. The high pressure gauge (0-4000 PSI) will indicate the pressure within the tank. The low pressure gauge (0-60 PSI) will indicate the output pressure on the supply hose to the incubator. Do not use single stage regulators. They will not supply a stable pressure. Just because a regulator has two gauges does not mean that it is a dual stage regulator. Insure
that you are using a dual stage regulator.
C. It is normal for the high pressure gauge on your regulator to start out reading 800 to 1000
PSI with a full tank. The reading will drop to 500 to 800 PSI quickly and will stay there for most of the duration of the tank. At the end of use the pressure will drop quickly to zero to indicate that the tank is completely empty. Pure CO2 is in a liquid state in the tank, and a constant vapor pressure is generated in the tank above the liquid level. The CO2 is drawn off of the top as a gas. The same vapor pressure is maintained as long as any liquid is left in the tank. When the last of the liquid has evaporated into gas then the pressure will drop rapidly as the gas is drawn off.
D. Only medical grade CO2 should be used in your incubator. The use of any lesser grade may
damage your incubator and void your warranty.
E. The micro processor CO2 control system interprets the information from the CO2 sensor,
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displays the CO2 concentration directly on the digital display, reads the CO2 set point and controls the percentage of CO2 in the incubator chamber.
F. The IR controlled unit is known as a direct sensor and operates under the principal that a
certain frequency of infrared light is absorbed by CO2. The more CO2 is present the more light is absorbed. Its accuracy is consistent no matter what the conditions are in the incubator. The IR controller utilizes an infared spectrophotometer to directly read the flow of CO2 gas into the chamber.
G. It is recommended that the accuracy of your CO2 control system be monitored by measuring
the actual CO2 concentration on a weekly basis with a Fyrite or other measuring device. This should be done when the chamber has not been disturbed for an extended period of time, i.e. after the weekend, or first thing on Monday morning.
5.10 Setting the CO2 Control: Attach the supply hose to the incubator and turn on the CO2 supply. Set the CO2 control to the desired set point using the procedure described in section 5.3.
5.11 Adjusting CO2 Display: After the incubator has had several hours to stabilize at CO2 set point, measure the actual CO2% with a Fyrite. If there is any difference between the Fryite and the display, use the procedure described in section 5.4 for making the display match the Fyrite. See Section 7.0 for Fyrite use.
NOTE: When using the Fyrite, insure that gas is not being injected while the reading is being taken. Changing the CO2 set point to 0.0 prior to taking the sample and change the set point back to the desired value after the use of the Fyrite is finished.
5.12 Audible Alarms: The temperature control and the CO2 control are both equipped with visual and audible alarms.
A. Temperature Alarms: The temperature control has alarm indicators for high and low
conditions that are activated whenever the actual temperature is 1 above or below the set point.
B. CO2 Alarms: The CO2 controller has alarm indicators for high and low alarm conditions that
are activated whenever the actual CO2% is 1% above or below the set point.
C. Audible Alarms: Both controls are equipped with audible alarms that are activated when
either of the HIGH or LOW indicators are activated. The audible alarms can be muted for a single alarm occurrence by pressing and holding down either the UP or DOWN button for several seconds until the alarm mutes. There is a built in delay of approximately 15 minutes on the occurrence of a LOW alarm. This time delay prevents the audible alarm from activating every time the door is opened and the temperature and CO2 drops.
5.13 Description of nGS (NO Gas Warning) Function: (If equipped)
The CO2 Low alarm activates 15 minutes after the CO2 level drops more than 1% below the set point. If the cause is not corrected in the next 5 minutes the CO2 display will flash nGS, indicating a no gas warning. nGS will continue to flash until the CO2 level is within 1% of set point (or above 4% if the set point is 5%). The audible alarm can be muted but the visual low alarm indicator will continue to be lit and the display will continue to flash nGS until the low alarm deactivates. No action is required by the user other than to correct the cause of the CO2 drop. Note: It may take several hours after loss of gas before the CO2 low alarm comes on. This depends on the unit’s natural rate of decay if no CO2 is injected. CO2 low alarm does not activate until CO2 has fallen more than 1% of set point (or below 4% if set point is 5%).
Restore gas supply. Check CO2 tank and gas supply lines to the unit. As soon as the gas supply is restored and the CO2 level rises to less than 1% below set point
(above 4.0% if set point is 5%) the display will stop blinking nGS and will again show the sensor reading. No action is required by the user other than correcting the cause of the drop in gas supply. The audible alarm can be muted but the visual low alarm indicator will continue to be lit and the display will continue to flash nGS until the low alarm deactivates.
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FYRITE CO2 CHECKING
Section
6
Section
6
A Bacharach FYRITE CO Gas Analyzer is recommended to measure CO concentrations in the incubator chamber. This test instrument is not supplied with the incubator but is readily available from your dealer. Follow the instructions provided with the Fyrite instrument carefully to insure correct and accurate readings.
6.1 Press button on top of Fyrite canister to release CO concentration. Tip canister to the side to ensure all fluid is released from the top of canister.
6.2 Loosen screw on slide scale and align top of fluid with zero on the scale. Tighten screw.
6.3 Connect hose and aspirator bulb to unit being tested. The sample port for connection is located in the upper front corner of the right side of the incubator.
6.4 Place the hose sampling cap directly over the plunger valve on top of canister and depress firmly.
6.5 With button depressed, squeeze bulb 27 times. On the last squeeze, with bulb still deflated, release hose from button.
6.6 Turn Fyrite canister upside down 3 times, each time allowing all fluid to flow to he opposite end of the canister.
6.7 Tip canister to the 45 degree position to ensure all fluid has been released from top of canister.
6.8 Read CO concentration in %.
NOTE: Your Fyrite indicator will come with a complete set of detailed instructions which should be followed carefully. The fluid used inside this Fyrite instrument is poisonous and corrosive and must not be taken internally. In event of a spill or accidental body contact with the Fyrite fluid, follow instructions on the refill bottle carefully.
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HEPA CO2 FILTER
Section
7
The Hepa filter attaches to a fixed coupling ring with a direct “snap-in” action. Push straight up to the coupling ring to connect. Pull the filter straight down to disengage. Replacement Hepa filters are available from Sheldon Mfg., Inc. Part number 2800517.
The CO2 Incubator design features a HEPA filtration system with a patented copper housing. (Patent No. 6,333,004) As the chamber air is drawn through the filter system, airborne microbes and isolated particulates are not only removed from the air, but destroyed as well. It is through copper oxidation that the contaminants are actually killed. The HEPA filter is easily replaced without the need of any tools and has an efficiency rating of 99.97% at 0.3 microns.
Install the HEPA Filter onto the duct prior to installing the duct in the unit.
WARNING: Ensure that when installing the duct to ensure that the duct is not touching the C02 sensor head as this may cause erroneous readings.
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MAINTENANCE
Section
8
NOTE: Prior to any maintenance or service on this unit, disconnect the service cord from the power supply.
8.1 Cleaning: Cleaning and decontamination are recommended on a regular basis. To prepare the incubator for cleaning remove all interior parts such as the shelf standards and false top. All stainless steel parts are autoclavable. Please review the Cleaning procedures described in the Installation section for detailed instructions.
8.2 Check CO2 supply periodically; don't let it run out. The internal auto tank switch is not alarmed.
8.3 CO2 supply lines and connections can be checked for leaks with a liquid soap solution. Apply liquid soap solution to all areas of the supply line and observe for bubbles that will indicate a leak.
8.4 Keep the CO2 flow system free of impurities. Erratic CO2 control is usually traceable to the CO2 pressure regulator on the tank, impurities in the tank, or impurities in the solenoid valve. Replace the CO2 in-line filters every six (6) months or when the filters have become noticeably dirty on the upstream side. There is a CO2 filter attached to the tubing kit, and one inside the shadow box connected to the GAS-IN line.
8.5 If the data logger fails to function as specified, check to verify that the port connections are solid and not loose. If the data logger still fails to function properly call Customer Service for assistance.
8.6 There is no maintenance required on any controls. If the controls fail to operate as specified, please review Section 9.0 Troubleshooting, prior to contacting customer service.
8.7 Storage: If the incubator is to be turned off for any length of time, dry the chamber and humidity pan thoroughly and leave at room temperature. Failure to do this may cause the interior to become contaminated. No adjustment to controls should be required when restarting the unit. If the unit is to be shut down for transport, drain the water jacket, remove humidity pan and shelves,
dry the chamber and disconnect the CO2 and power supply’s. See section 3.3, Lifting / Handling
for transport procedures.
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TROUBLESHOOTING
TEMPERATURE
Temperature too high
1/ controller set too high-see section 5.3 2/ controller failed on – call Customer Service 3/ wiring error – call Customer Service
Display reads "HI" or "400"+
probe is unplugged, is broken or wire to sensor is broken – trace wire from display to probe; move wire and watch display to see intermittent problems
Chamber temp spikes over set point and then settles to set point
recalibrate – see section 5.4
Temperature too low
1/ OTP too low – see section 5.8.E 2/ controller set too low – see section 5.3 3/ unit not recovered from door opening – wait for display to stop changing 4/ unit not recovered from power failure or being turned off – incubators will need 24 hours to warm up and stabilize 5/ element failure – see if heating light is on; compare current draw to data plate 6/ controller failure – confirm with front panel lights that controller is calling for heat 7/ OTP failure – confirm with front panel lights that it is operating correctly 8/ wiring problem – check all functions and compare wiring to diagram in section 10.0, especially around any areas recently worked on 9/ loose connection – check shadow box for loose connections
Display reads "LO"
1/ sensor is plugged in backwards – reverse sensor wires to controller 2/ if ambient room temperature is lower than range of unit – compare set points and ambient temperature to rated specifications in section 9.0
Unit will not heat over a temperature that is below set point
1/ confirm that fan is moving and that amperage and voltage match data plate – check fan motor motion in shadow box and feel for air movement in chamber 2/ confirm that set point is set high enough ,turn OTP counterclockwise and see if heating light or safety light comes on 3/ check connections to sensor 4/ check calibration – using independent certified reference thermometer, follow instructions in sections 5.4 and 5.8
Unit will not heat up at all
Section
9
CAUTION: Extreme caution must be exercised any time access is made into areas housing electrical components. Repair, replacement or adjustment of components in these areas must only be done by qualified technicians familiar with electrical circuitry and the operation of the chamber.
Always make a visual inspection of the incubator and control console when troubleshooting. Look for loose or disconnected wires or tubing, which may be the source of the trouble.
NOTE: IF BREAKERS TRIP REPEATEDLY, OR FUSES BLOW REPEATEDLY, CALL QUALIFIED SERVICE PERSONNEL.
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1/ verify that controller is asking for heat by looking for controller light – if pilot light is not on continuously during initial start up there is a problem with the controller 2/ check amperage – amperage should be virtually at maximum rated (data plate) amperage 3/ do all controller functions work? 4/ is the OTP set high enough? – for diagnostics, should be fully clockwise with the pilot light never on 5/ has the fuse/circuit breaker blown?
Indicated chamber temperature unstable
1/ ±0.1 may be normal 2/ is fan working? – remove top panel and verify movement of cooling fan in center of shadow box 3/ is ambient room temperature radically changing – either door opening or room airflow from heaters or air conditioning? – stabilize ambient conditions 4/ sensor miss-located, damaged, or wires may be damaged - check mounts for control and OTP sensors, then trace wires between sensors and controls 5/ calibration sensitivity – call Customer Service 6/ OTP set too low – be sure that OTP set point is more than 5 degrees over desired Main set point; check if OTP pilot is on continuously; turn controller knob completely clockwise to see if problem solved then follow instructions in section 5.8.E for correct setting. 7/ electrical noise – remove nearby sources of RFI including motors, arcing relays or radio transmitters 8/ bad connection on temperature sensor or faulty sensor – check connectors for continuity and mechanical soundness while watching display for erratic behavior; check sensor and wiring for mechanical damage 9/ bad connections or faulty capacitor – check connectors for mechanical soundness and look for corrosion around terminals or signs of arcing or other visible deterioration
Will not maintain set point
1/ assure that set point is at least 5 degrees over ambient room temperature 2/ see if ambient is fluctuating
Display and reference thermometer don’t match
1/ calibration error – see section 5.4 2/ temperature sensor failure – evaluate if pilot light is operating correctly 3/ controller failure – evaluate if pilot light is operating correctly 4/ allow at least two hours to stabilize 5/ verify that reference thermometer is certified
Can't adjust set points or calibration
1/ turn entire unit off and on to reset 2/ if repeatedly happens, call Customer Service
Calibrated at one temperature, but not at another
This can be a normal condition when operating temperature varies widely. For maximum accuracy, calibration should be done at or as close to the set point temperature.
CO2 LEVEL
Overshoots set point but stabilizes ­display and Fyrite match
19
1/ turn set point up and down to see if solenoid valve works by feeling and listening to valve 2/ recalibrate with Fyrite, see section 5.11 and section 7.0 3/ fan not operating correctly a- fan motor stopped b- fan blade fell off c- wrong fan blade installed or mounted backwards 4/ wrong restrictor installed or missing altogether 5/ tank pressure too high, see section 5.9.A 6/ CO2 sensor partially plugged with dirt or condensation 7/ regulator set wrong, see section 5.9 8/ incubator too heavily loaded 9/ incubator being operated without shelving 10/ CO2 attached to sample port instead of “CO2 IN” fitting 12/ if display and Fyrite does not match, see Troubleshooting Co2-5
Overshoots set point and continues to rise
- display and Fyrite match
1/ debris in solenoid causing it to leak continuously 2/ solenoid failed while open 3/ unit miss-wired 4/ controller output failed or shorted 5/ CO2 sensor or interface failure 6/ CO2 sensor plugged by debris or condensation
Rises very slowly
1/ restrictor partially plugged 2/ filter overly dirty or partially plugged 3/ CO2 tank regulator set too low, see section 5.9 4/ hose kinked or leaking 5/ poor door seal 6/ CO2 tank contains mixed gas, not 100% medical grade CO2
Never rises
1/ CO2 tank empty 2/ solenoid failed while closed 3/ CO2 controller output failed while open 4/ restrictor plugged 5/ CO2 hose blockage 6/ CO2 filter plugged 7/ set point is at 0.0 and has not been reset, see section 5.3 and 5.4
Display and Fyrite reading do not match
1/ calibration error - clear chamber for 12 hrs and confirm at "0" 2/ turn set point up and down to see if solenoid valve works by feeling and listening to valve 3/ assure that IR tube block is completely tight, 24 series 4/ if display is varying when doing Fyrite test, have a leak on IR base or mounting plate, or CO2 is injecting at time of test. 5/ take voltage reading across sensor leads 0=0V and 20=1V 6/ assure that sensor is properly mounted 7/ DISPLAY LOWER THAN FYRITE: air leak around the CO2 sensor or the fan motor shaft 8/ DISPLAY HIGHER THAN FYRITE: leak in tube from sample port to chamber, verify with liquid soap solution. 9/ DISPLAY HIGHER THAN FYRITE: Fyrite fluid worn out, replace fluid with Fyrite refill. 10/ DISPLAY HIGHER THAN FYRITE: poor Fyrite sampling technique, see section 6.0 11/ DISPLAY HIGHER THAN FYRITE: defective Fyrite pump or tubing 12/ CO2 sensor, interface or controller failure
Is unstable – display or actual reading varies around set point
20
1/ confirm that fan is working 2/ check for air leak around CO2 sensor or fan motor shaft 3/ magnetic field interference 4/ door not sealing properly 5/ atmospheric pressure fluctuations 6/ defective Fyrite pump or tubing 7/ CO2 entering chamber too quickly a- tank pressure too high b- regulator set wrong c- restrictor incorrect or missing 8/ electronic problem with CO2 sensor, interface or controller 9/ top of unit exposed to cold air drafts 10/ unit being operated without shadow box cover 11/ electrical interference – atmospheric or conducted 12/ incubator too heavily loaded
Can't adjust set points or calibration ­"locked up"
1/ turn unit off and on to reset processor in controller 2/ if repeatedly happens, call Customer Service
Feeding continuously or abnormally high CO2 usage
1/ do decay test: if more than 1% decay in an hour, check for leak : door gasket tightness, motor inlet to chamber, sensor or probe inlet to chamber, chamber access tube plugged 2/ check output signal from controller 3/ check solenoid valve for correct operation 4/ leak in plumbing including between regulator and CO2 tank 5/ door being opened too often
Won’t hold calibration – Fyrite reading varies but display stable
1/ atmospheric pressure fluctuations 2/ top of unit exposed to cold air drafts 3/ unit being operated without shadow box cover in place 4/ condensation collecting on CO2 sensor 5/ CO2 sensor or interface failure 6/ unit incorrectly calibrated, see section 5.4 7/ taking Fyrite reading too soon after the door has been opened 8/ air leak around CO2 sensor mounting plate
Low CO2 alarm activated and nGS (NO Gas warning) flashing on CO2 display.
CO2 gas supply has been interrupted or run out. Tank ran out of gas or did not switch over. Pinched gas line tubing Severe leak in gas line Tubing is disconnected from the gas supply..
Low CO2 alarm activated and nGS (NO Gas warning) flashing on CO2 display.
CO2 gas supply has been interrupted or run out. Tank ran out of gas or did not switch over. Pinched gas line tubing Severe leak in gas line Tubing is disconnected from the gas supply.
MECHANICAL
Door not sealing
1/ check physical condition of gasket 2/ confirm that door latch pulls door in tightly 3/ assure that gasket is in original location
Motor doesn't move
21
1/ if shaft spins freely: check connections to motor and check voltage to motor; 2/ if shaft rubs or is frozen, relieve binding and retest
Motor makes noise
1) Make sure that the fan or blower wheel is not contacting its housing. Adjust the motor mounting bracket position to re-center the fan or blower wheel, if necessary.
2) Check the fan or blower wheel for damage or out of balance condition. Replace the fan or blower wheel if it is damaged or out of balance.
3) Turn the motor shaft to make sure that it spins freely. If it binds or the bearings make a rubbing or scrapping sound then replace the motor.
Solenoid valve buzzing
After removing solenoid clean with alcohol for carbon build-up then blow out. Check valve seat or channels for contamination. Check CO2 filter and/or grade of CO2 used. If seat is worn, replace.
OTHER
Controller on at all times - "locked-up"
1/ turn unit off and on to reset 2/ if cannot change any condition on the front panel, call Customer Service
Front panel displays are all off
1/ Check for wire damage.
Unit or wall fuse/circuit breaker is blown
1/ check wall power source 2/ compare current draw and compare to specs on data plate 3/ see what other loads are on the wall circuit
Unit will not turn on
1/ check wall power source 2/ check fuse/circuit breaker on unit or in wall 3/ see if unit is on, e.g., fan or heater, and just controller is off 4/ check all wiring connections, esp. around the on/off switch
Unit is smoking – Out of box
Put unit under vent and run at full power for one hour.
Contamination in chamber
1/ see cleaning procedure in Installation section 3.0 2/ develop and follow Standard operating procedure for specific application; include definition of cleaning technique and maintenance schedule.
NOTE: THE TEMPERATURE OF THE GLASS DOOR AND THE INTERIOR CHAMBER WALLS MUST BE EQUAL TO PREVENT CONDENSATION.
The incubator is designed so that no internal electrical servicing should be required under normal conditions. If electrical servicing is necessary, it should be performed by qualified service personnel. FOR PERSONAL SAFETY, ALWAYS DISCONNECT THE POWER BEFORE SERVICING.
22
PARTS LIST
Description
SCO2W
115V
SCO2W-2
220V
Anode
0260500
0260500
Cable Panel Mount USB Type B Extention
1800533
1800533
CO2 Display Board
1750735
1750735
CO2 Filter
2800525
2800525
CO2 Pre-Heater
2350546
2350551
CO2 Sensor w/ Interface
9700501
9700501
Cord Set
1800510
1800539
Cord Set - European
NA
1800500
Door Heater
2350550
2350550
Door Switch
7850578
7850578
Element Coil
2350503
2350554
Fan Blade
2600543
2600543
Fan Motor
4880508
4880507
Glass Door Latch
9530516
9530516
Gasket
3450645
3450645
Humidity Pan
995-00015
995-00015
IR Transmitter
8320510
8320510
Leveling Feet
2700512
2700512
Overtemperature Safety
1750860
1750860
Pod Control 4 Loop
1750725
1750725
Power Inlet Fuse
3300516
3300515
Power Inlet
4200522
5200522
Power Switch
7850532
7850532
Shelf
5071131
5071131
SL Logo Slug
5800529
5800529
Solenoid Valve
8600528
8600529
Temperature Display Board
1750734
1750734
USB to RS232 Bridge Board
1750846
1750846
Water Level Switch
7850575
7850575
Section
10
23
UNIT SPECIFICATIONS
Weight
Shipping
Net
SCO2W SCO2W-2
(3503 3503-2)
175 lbs.
140 lbs.
Dimensions
Exterior WxDxH (IN.)
Interior
WxDxH (IN.)
SCO2W SCO2W-2
(3503 3503-2)
20x 21.6 x 26.5
15.8 x 15.8 x 12.5
Capacity
Cubic Feet
SCO2W SCO2W-2
(3503 3503-2)
1.8
Temperature
Range
Uniformity
CO2
SCO2W SCO2W-2
(3503 3503-2
Amb. 8C to 60C
+0.2C @ 37C
0 – 20%
Model
Amp
Voltage
Hertz
SCO2W (3503)
5 amp
110-120V
50/60 Hz
SCO2W-2 (3503-2)
3.5 amp
208-240V
50/60 Hz
24
FIGURE 5
M
GREEN CHERRY
ROUND
POWER SWITCH
SL# 7850532
POWER INLET 6A SL# 104179
WATER FILL
SWITCH SL# 210004
WATER FILL INDICATOR
SL# 200020
CO2 PREHEATER SL# 100909
6W
HEATING INDICATOR SL# 200021
OTP SL# 100001J
OTP INDICATOR
SL# 200020
MAIN HEATING ELEMENT
SL# 2350503
DOOR HEATERS
SL# 103068
10W
10W 10W
10W
CO2 INDICATOR
SL# 200021
CO2 SOLENOID
SL# 8600528
CIRCULATING FAN
MOTOR SL# 4880508
4 LOOP CONTROL
BOARD SL# 1750730
J8
MAIN TEMP
SENSOR
DOOR
SENSOR
J9
CO2
SENSOR
J17
HUMIDITY SENSOR
J16
BUSS
LOAD 1
LOAD 2
LOAD 3
LOAD 4
J1
J2
J3
J4
J5
J6
J7
EARTH GRD
(NEUT)
(HOT)
J10
COMMUNICATION
ALARM CONTACTS
IR CO2 SENSOR
SL# 8320510
12VDC
POWER SUPPLY
SL# 6750507
DOOR SWITCH
SL# X1000022
SL LOGO
LIGHT BOARD
SL# 1750737
ALARM JACK
SL# 100163
HOT TERMINAL BLOCK
NEUTRAL TERMINAL BLOCK
TEMP
DISPLAY
SL# 1750734
CO2
DISPLAY
SL# 1750735
USB BOARD SL# 1750846
USB EXSTENSION
CABLE SL# 1800533
.47uf
BLACK
BLACK
BLACK
BLACK
WHITE
WHITE
WHITE
WHITE
WHITE
WHITE
WHITE
WHITE
WHITE WHITE
WHITE
WHITEWHITE
ORANGE
ORANGE
YELLOW
YELLOW
RED
BLUE
BLACK
BLACK
WHITE
RED
BLACK
WH HT
BL HT
WHITE
BLACK
WIRING DIAGRAM
SCO2W (3503) 110V
25
WIRING DIAGRAM
M
GREEN CHERRY
ROUND
POWER SWITCH
SL# 7850532
FUSED POWER INLET
6A SL# 104179
WATER FILL
SWITCH SL# 210004
WATER FILL INDICATOR
SL# 200020
CO2 PREHEATER SL# 103328
6W
HEATING INDICATOR SL# 200021
OTP SL# 100001J
OTP INDICATOR
SL# 200020
MAIN HEATING ELEMENT
SL# 120071
DOOR HEATERS
SL# 103068
10W 10W 10W
10W
CO2 INDICATOR
SL# 200021
CO2 SOLENOID
SL# 8600529
CIRCULATING FAN
MOTOR SL# 4880507
4 LOOP CONTROL
BOARD SL# 1750730
J8
MAIN TEMP
SENSOR
DOOR
SENSOR
J9
CO2
SENSOR
J17
HUMIDITY
SENSOR
J16
BUSS
LOAD 1
LOAD 2
LOAD 3
LOAD 4
J1
J2
J3
J4
J5
J6
J7
EARTH GRD
(NEUT)
(HOT)
J10
COMMUNICATION
ALARM CONTACTS
IR CO2 SENSOR
SL# 8320510
12VDC
POWER SUPPLY
SL# 6750507
DOOR SWITCH
SL# X1000022
SL LOGO
LIGHT BOARD
SL# 1750737
ALARM JACK
SL# 100163
HOT TERMINAL BLOCK
NEUTRAL TERMINAL BLOCK
TEMP
DISPLAY
SL# 1750734
CO2
DISPLAY
SL# 1750735
USB BOARD SL# 1750846
USB EXSTENSION
CABLE SL# 1800533
.47uf
BLACK
BLACK
BLACK
BLACK
WHITE
WHITE
WHITE
WHITE
WHITE
WHITE
WHITE
WHITE
WHITE WHITE
WHITE
WHITEWHITE
ORANGE
ORANGE
YELLOW
YELLOW
RED
BLUE
BLACK
BLACK
WHITE
RED
BLACK
WH HT
BL HT
WHITE
BLACK
WHITE
FUSE HOLDER
SL# 3300501
SCO2W-2 (3503-2) 220V
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