Shel Lab SCO2W User Manual
Installation and
Operation Manual
SCO2W
Previously Designated:
3502 & 3503
WATER JACKETED CO2 INCUBATOR
110 – 120 Voltage
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SCO2W WATER JACKETED CO2 INCUBATORS
110 – 120 Voltage
Installation and Operation Manual
Part Number (Manual): 4861740
Revision: January 19, 2017
These units are TÜV listed as water jacket incubators for professional, industrial, or educational use
where the preparation or testing of materials is done at an ambient air pressure range of 22.14 –
31.3 inHg (75 – 106 kPa), with no flammable, volatile, or combustible materials being heated.
These units have been tested to the following requirements:
CAN/CSA C22.2 No. 61010-1:2012
CAN/CSA C22.2 No. 61010-2-010:2004 Reaffirmed: 2014-07
UL 61010-1:2012-05
UL 61010A-2-010:2002-03
EN 61010-1:2010
EN 61010-2-010:2014
Supplemented by: UL 61010-2-010:2015
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TABLE OF CONTENTS
INTRODUCTION ........................................................................................................................................... 6
General Safety Considerations ................................................................................................................. 6
Engineering Improvements ....................................................................................................................... 7
Contacting Assistance ............................................................................................................................... 7
RECEIVING YOUR UNIT .............................................................................................................................. 8
Inspect the Shipment ................................................................................................................................ 8
Orientation Photo ...................................................................................................................................... 9
Regulator ................................................................................................................................................. 10
Recording Data Plate Information ........................................................................................................... 10
Reference Sensor Devices ..................................................................................................................... 10
INSTALLATION .......................................................................................................................................... 11
Installation Checklist ............................................................................................................................... 11
Required Ambient Conditions ................................................................................................................. 12
Environmental Disruption Sources .......................................................................................................... 12
Power Source Requirements .................................................................................................................. 12
Lifting and Handling ................................................................................................................................ 13
Install Incubator in Location .................................................................................................................... 13
Leveling ................................................................................................................................................... 13
Deionized and Distilled Water ................................................................................................................. 13
Installation - Clean and Disinfect ............................................................................................................. 14
Install Chamber HEPA Filter and Duct .................................................................................................... 14
Shelving Installation ................................................................................................................................ 15
Connect to the CO2 Supply ..................................................................................................................... 16
Access Port Stopper ............................................................................................................................... 17
Fill the Water Jacket ................................................................................................................................ 17
GRAPHIC SYMBOLS ................................................................................................................................. 18
CONTROL PANEL OVERVIEW ................................................................................................................. 20
OPERATION ............................................................................................................................................... 22
Theory of Operation ................................................................................................................................ 22
Put the Incubator into Operation ............................................................................................................. 26
Humidify the Incubator ............................................................................................................................ 27
Set the Temperature Set Point ................................................................................................................ 28
Muting the Audible Temperature Alarm .................................................................................................. 29
Automatic Door Cutoff ............................................................................................................................. 29
Set the CO2 Set Point .............................................................................................................................. 30
Muting the Audible CO2 Alarm ................................................................................................................ 31
No Gas Supply Alarm (NGS) .................................................................................................................. 31
Temperature Accuracy Verification ......................................................................................................... 32
CO2 Accuracy Verification ....................................................................................................................... 34
Set the Over Temperature Limit .............................................................................................................. 36
Load the Incubator .................................................................................................................................. 37
Accessory Compatibility .......................................................................................................................... 37
Data Output Capabilities ......................................................................................................................... 37
Condensation and the Dew Point ............................................................................................................ 38
USER MAINTENANCE ............................................................................................................................... 40
Cleaning and Disinfecting ....................................................................................................................... 40
Minimizing Contamination Exposure ....................................................................................................... 41
Gas Lines and HEPA Filters ................................................................................................................... 42
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Storage of the Incubator .......................................................................................................................... 42
Maintaining Atmospheric Integrity ........................................................................................................... 42
Electrical Components ............................................................................................................................ 42
Replace the Chamber HEPA Filter ......................................................................................................... 43
Calibrate the Temperature display .......................................................................................................... 44
Calibrate the CO2 Display ........................................................................................................................ 48
Anode and Water Quality ........................................................................................................................ 52
UNIT SPECIFICATIONS ............................................................................................................................. 54
Weight ..................................................................................................................................................... 54
Dimensions .............................................................................................................................................. 54
Capacity .................................................................................................................................................. 55
CO2 .......................................................................................................................................................... 55
Temperature ............................................................................................................................................ 55
Power ...................................................................................................................................................... 55
Pressure Conversion Table ..................................................................................................................... 55
PARTS LIST ............................................................................................................................................... 56
Ordering Parts and Consumables ........................................................................................................... 57
Accessories ............................................................................................................................................. 57
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INTRODUCTION
Thank you for purchasing a Shel Lab product. We know that in today’s competitive marketplace
customers have many choices when it comes to constant temperature equipment. We appreciate
you choosing ours. Our continued reputation as a leading laboratory product manufacturer rests
with you. We stand behind our products and will be here for you if you need us.
These incubators are intended for laboratory, industrial, and educational microbiological cultivation
applications. These incubators are not intended for use in hazardous or household locations.
Before using the unit read this manual in its entirety to understand how to install, operate, and
maintain the incubator in a safe manner. Keep this manual available for use by all operators.
Ensure that all operators are given appropriate training before the incubator begins service.
GENERAL SAFETY CONSIDERATIONS
Note: Failure to follow the guidelines and instructions in this manual may create a protection
impairment by disabling or interfering with the unit safety features. This can result in injury or
death.
Your unit and its recommended accessories are designed and tested to meet strict safety
requirements. It is designed to connect to a power source using the specific power cord type
shipped with the unit.
For continued safe operation of your unit, always follow basic safety precautions including:
Always plug the unit power cord into a protective earth grounded electrical receptacle
(outlet) that conforms to national and local electrical codes. If the unit is not grounded
properly, parts such as knobs and controls can conduct electricity and cause serious injury.
Do not bend the power cord excessively, step on it, or place heavy objects on it.
A damaged cord can be a shock or fire hazard. Never use a power cord if it is damaged or
altered in any way.
Always position the unit so that end-users can quickly unplug it in the event of an
emergency.
Do not attempt to move the unit while in operation or before the unit has cooled.
Do not stack the unit without a factory-approved stacking rack or adaptor.
Use only approved accessories. Do not modify system components. Any alterations or
modifications to your incubator can be dangerous and void your warranty.
Follow all local ordinances in your area regarding the use of this unit. If you have any
questions about local requirements, please contact the appropriate agencies.
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INTRODUCTION (CONTINUED)
ENGINEERING IMPROVEMENTS
Sheldon Manufacturing continually improves all of its products. As a result, engineering changes
and improvements are made from time to time. Therefore, some changes, modifications, and
improvements may not be covered in this manual. If your unit operating characteristics or
appearance differs from those described in this manual, please contact your Shel Lab dealer or
distributor for assistance.
CONTACTING ASSISTANCE
If you are unable to resolve a technical issue with the incubator, please contact Sheldon Technical
Support. Phone hours for Technical Support are 6 am – 4:30 pm Pacific Coast Time (west coast of
the United States, UTC -8). Please have the following information ready when calling or emailing:
the model number and the serial number (see page 9).
EMAIL: tech@shellab.com PHONE: 1-800-322-4897 extension 4, or (503) 640-3000 FAX: (503)
640-1366
Sheldon Manufacturing INC.
P.O. Box 627
Cornelius, OR 97113
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1 Copper Pan Token
1 Access Port*
Stopper
1 Ceiling Air Duct
1 Chamber HEPA
Filter
1 Chamber Filter Cap
5800529
7750514
5071323
2800517
6500506
1 CO2 Tubing Kit
1 Fill Port
Stopper*
1 Humidification
Pan
4 Leveling Feet
1 Power Cord
9710500
7750513
995-00015
2700512
1800510
3 Shelves
6 Shelf Slides
4 Shelf Standards
5071131
5071144
5170648
RECEIVING YOUR UNIT
INSPECT THE SHIPMENT
When a unit leaves the factory, safe delivery becomes the responsibility of the carrier.
Damage sustained during transit is not covered by the manufacturing defect warranty.
When you receive your unit, inspect it for concealed loss or damage to its interior and exterior. If
you find any damage to the unit, follow the carrier’s procedure for claiming damage or loss.
1. Carefully inspect the shipping carton for damage.
2. Report any damage to the carrier service that delivered the unit.
3. If the carton is not damaged, open the carton and remove the contents.
4. The unit should come with an Installation and Operation Manual.
5. Verify that the correct number of accessories have been included.
Included Accessories SCO2W
*The access and water fill stoppers ship installed in their respective ports on the unit.
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Figure 1:
SCO2W
Back of Unit
CO2 In Port
Fuse in Power Inlet
Chamber
Gasket
Ceiling Air
Duct
Chamber
HEPA Filter
Door Latch
Humidification Pan
Viewing Door
(Glass)
Chamber Door
Viewing Door Knob
Access Port
(Side of Unit)
Door Gasket
Back of Unit
Fill Port
Control Panel
Water-Jacket Ports
Siphon Port
Anode Top
Right Side of Unit
CO2 Sample and USB-Style Ports
RECEIVING (CONTINUED)
ORIENTATION PHOTO
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Model Number
Serial Number
Figure 2: CO2 Regulator
RECEIVING (CONTINUED)
REGULATOR
The incubator must be connected to either a building CO2 gas supply system or a
supply cylinder (tank). A cylinder regulator is not included with the incubator and
must be purchased separately. Please see the Accessories section on page 57 if
you wish to order one from Shel Lab.
RECORDING DATA PLATE INFORMATION
Locate the data plate inside the incubation chamber, on the top right corner of the chamber door.
The data plate contains the incubator model number and serial number. Enter this information
below for future reference.
Data Plate Information
REFERENCE SENSOR DEVICES
Reference sensor devices or a combined device must be purchased separately in order to perform
accuracy verifications or calibrations of the incubator temperature and CO2 displays.
Reference devices must be accurate to at least 0.1°C and 0.1% CO2 For best temperature results,
use a digital device with a wired-connected temperature sensing probe that can be placed in the
incubation chamber through the unit access port. For example, a wire thermocouple probe. For
best CO2 accuracy, use a calibrated digital gas analyzer with sample tubing that can be connected
to the incubator external CO2 sample port. The devices should be regularly calibrated, preferably by
a third party.
Reference readings that avoid chamber door openings during verification and calibration eliminate
subsequent waits for the chamber temperature and gas levels to re-stabilize before proceeding.
This also allows temperature and gas verifications or calibrations to be performed simultaneously.
Select probes suitable for the application temperature you will be calibrating or verifying the
incubator displays at.
Alcohol thermometers are insufficient for conducting accurate temperature verifications and
calibrations. Do not use a mercury thermometer. Never place a mercury thermometer in the
incubation chamber.
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INSTALLATION
INSTALLATION CHECKLIST
Carry out the steps and procedures listed below to install the unit in a new workspace location and
prepare it for use. All procedures are found in the Installation section of this manual.
Pre-Installation
Procure a CO
Always use medical grade CO2.
gas supply for the incubator with a concentration suitable for your application.
2
Check that the required ambient conditions, ventilation, and spacing for the incubator are met,
page 12.
Unit dimensions may be found on page 54
Check for performance-disrupting heat and cold sources in the environment, page 12
Check that a suitable electrical outlet and power supply is present, page 12
Install the Incubator in a suitable location
Review the lifting and handling instructions, page 13
Install the incubator in its workspace location, page 13
Make sure the incubator is level, page 13
Set up the Incubator for use
Clean and disinfect the incubator and accessories (except the HEPA filter) that will be placed
in the incubation chamber, page 14
Do not use deionized water to clean the unit or fill the water jacket, see page 13
Install the chamber HEPA filter and ceiling air duct in the incubation chamber, page 14
Install the shelving in the incubation chamber, page 15
Connect the incubator to the CO
gas supply source, page 16
2
Verify that the rubber stopper is installed in the access port inside the incubation chamber,
page 17
Fill the water jacket with 9.5 gallons (36 liters) of water, page 17
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INSTALLATION (CONTINUED)
REQUIRED AMBIENT CONDITIONS
SCO incubators are intended for use indoors at room temperatures between 15C and 30C (59F
and 86F), at no greater than an ambient 80% Relative Humidity (at 25C / 77F).
4 inches (10cm) minimum ventilation clearance between the incubator and walls or partitions.
2 inches (5cm) minimum clearance above the top of the incubator for unobstructed airflow.
Operating the unit outside these conditions may adversely affect its temperature range and
stability. For conditions outside of those listed above, please contact your distributor to explore
other unit options suited to your laboratory or production environment.
ENVIRONMENTAL DISRUPTION SOURCES
When selecting a location to install the unit, consider all environmental conditions that can affect its
temperature performance. For example:
Proximity to ovens, autoclaves, and any device that produces significant radiant heat
Heating and cooling ducts, or other sources of fast-moving air currents
High-traffic areas
Direct sunlight
POWER SOURCE REQUIREMENTS
When selecting a location for the unit, verify that each of the following requirements is satisfied:
The wall power outlet must meet the power requirements listed on the unit data plate. This unit is
intended for 110 – 120 VAC 50/60 Hz applications at 5.0 amps.
Wall power sources must be protective earth grounded. Supplied voltage must not vary
more than 10% from the data plate rating. Damage to the unit may result if supplied
voltage varies more than 10%.
Wall power sources must conform to all national and local electrical codes.
Use a separate circuit to prevent loss of product due to overloading or circuit failure. The
circuit must match or exceeded the amperage requirement listed on the unit the data plate.
The unit must be positioned so that all end-users can quickly unplug the power cord in the event of
an emergency.
The unit is provided with an 115 volt 15 Amp, 9ft 5 in (2.86m) NEMA 5-15P power cord.
The unit is provided with a 250V time-lag, 10 amp, 5x20mm fuse located in the power inlet.
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Figure 3:
Leveling Foot
INSTALLATION (CONTINUED)
LIFTING AND HANDLING
The unit is heavy. Use appropriate powered lifting devices. Follow these guidelines when lifting and
handling the unit:
Lift the unit only from its bottom surface.
Doors, handles, and knobs are not adequate for lifting or stabilization.
Restrain the unit completely while lifting or transporting so it cannot tip.
Remove all removable components, such as shelving, and secure all doors in the closed
position during transfer to prevent shifting and damage.
INSTALL INCUBATOR IN LOCATION
Install the unit in a workspace location that meets the criteria discussed in the previous entries of
the Installation section.
LEVELING
Install the leveling feet shipped with the unit. The unit must be level and stable for safe
operation.
Insert one leveling foot into each of the four holes in the bottom corners of the
unit.
Adjust the foot at each corner until the unit stands level and solid without rocking.
To raise a foot, turn it in a counterclockwise direction.
To lower a foot, turn it in a clockwise direction.
Note: To prevent damage when moving the unit, turn each of the four leveling feet completely
clockwise.
DEIONIZED AND DISTILLED WATER
Do not use deionized water to clean or humidify the incubator, or fill the water jacket. Use of
deionized water may corrode metal surfaces and voids the warranty. The manufacturer
recommends the use of distilled water in the resistance range of 50K Ohm/cm to 1M Ohm/cm, or a
conductivity range of 20.0 uS/cm to 1.0 uS/cm, for cleaning, humidifying, and water-jacketing
applications.
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Chamber
Ceiling Air
Duct
Step 1
Chamber
HEPA Filter
Filter Cap
Back
Step 2
Step 3
Step 5
Front
Front
Mounting Pin
Step 4
Back
Rear Mounting
Pin
INSTALLATION (CONTINUED)
INSTALLATION - CLEAN AND DISINFECT
Cleaning and disinfecting the incubation chamber, shelving
components and ceiling air duct now reduces the risk of
contamination. The chamber and shelving were cleaned and
disinfected at the factory, however, the unit may have been exposed
to contaminants during shipping.
Remove all protective wrappings from shelving components
and the ceiling air duct prior to cleaning.
Do not clean the chamber HEPA filter!
See the Cleaning and Disinfecting entry on page 40 for
information on how to clean and disinfect without damaging
the incubator or its components.
INSTALL CHAMBER HEPA FILTER AND DUCT
Note: Exercise caution to avoid striking the sensors and blower fan
wheel on the chamber ceiling when installing the duct.
Note: The incubator must be turned off and unplugged when carrying
out this procedure.
The ring-style chamber HEPA filter traps particulates, as well as
isolating and killing airborne microbes in the incubation chamber.
Perform the following steps to install the filter and ceiling air duct.
Snap the chamber HEPA filter to the mounting collar on the
chamber ceiling air duct.
a. It may be necessary to tilt the filter to one side or the
other.
Snap the filter cap to the HEPA filter.
Move the air duct into the incubation chamber with the
attached HEPA filter facing down.
Mount the back of the air duct on the rear chamber mounting
pins, one pin at a time.
Mount the front of the duct on the front chamber mountings,
one at a time.
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INSTALLATION (CONTINUED)
SHELVING INSTALLATION
Note: Always install the copper token in the humidification pan. Copper is known to have
antimicrobial properties that retard the growth of microorganisms in the pan.
Install the shelving and humidification pan in the in the
incubation chamber.
Install the shelf standard rails.
a. Align the keyhole slot of the standard with the
mounting peg on the side of the chamber wall.
b. Mount the shelf standard.
Install the shelf slides.
a. Insert the shelf slide into the shelf standard
using a rocking motion.
b. The shelf slide will sit level when correctly
installed.
Install the shelves.
a. Slide into position.
Install the humidification pan.
a. Place the copper token in the humidity pan.
b. Secure the token using the clip on the bottom
of the pan.
c. Place the pan on the chamber floor.
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PSI
Megapascals
Kilopascals
Bar
15 - 20 psi
0.103 - 0.137 Mpa
103.42 - 137.89 Kpa
1.03 - 1.378 bar
Figure 6: CO2
to Chamber
Incubator
Figure 5: Gas
Tubing Kit
Figure 4: CO2 Cylinder
Connection
CO2 Regulator
HEPA Filter
INSTALLATION (CONTINUED)
CONNECT TO THE CO
Note: Always use medical grade CO2. Use of non-medical grade CO2 risks introducing
contaminants into the chamber may damage the incubator and will void the manufacturing
defect warranty.
The incubator may be connected to either a building supply source or a supply cylinder.
Two-Stage Regulators: If connecting to a supply cylinder always use a two-stage CO2 pressure
regulator. Be aware that some single-stage regulators have 2 gauges. Make certain your regulator
is a two-stage regulator. Precise regulation of the gas input flow is vital for the incubator
performance.
2
SUPPLY
Connect to the CO2 supply
Attach the CO2 regulator to a medical grade CO2 cylinder, if using a cylinder supply.
Set the wall source control or cylinder regulator to 15 - 20 Pounds per Square Inch (psi).
Do not exceed 25 psi.
Remove the dust cover from the CO2–to-Chamber port on the back of the unit.
Connect the gas tubing to the incubator and regulator or wall source.
a. Connect the black tubing to the regulator or wall source.
b. Connect the clear tubing to the CO2 to Chamber port on the back of the
incubator.
Do not start a flow of CO2 to the incubator at this time.
End of procedure
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Figure 7: Port Stopper
in the Access Port
Fill Port
9 Gallons
(36 Liters)
INSTALLATION (CONTINUED)
ACCESS PORT STOPPER
Verify the port stopper is installed in the access port on the right side of the
unit. The unit will not meet its temperature performance specifications or
maintain a CO2 set point concentration without the stopper installed.
The stopper must be installed inside the chamber for the unit to obtain the
meet its temperature specifications and prevent condensation from forming
inside the port.
FILL THE WATER JACKET
The water jacket requires 9.5 gallons (36 liters) to provide sufficient thermal insulation for the incubator
to operate at its stated temperature specifications.
The manufacturer recommends the use of distilled water or
filtered tap water if possible. Please see the Anode and
Water Quality entry in the maintenance section on page 52.
Use of a funnel or hose while filling the jacket is strongly
recommended.
Locate the water fill port on the back right side of the
incubator.
Remove the port stopper.
a. Place the port stopper in a location where it will be easy to
find.
Fill the jacket with 9.5 gallons (36 liters) by pouring water in
through the fill port.
Place the stopper back in the port.
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Symbol
Definition
Indicates that you should consult your user manual for further instructions.
Indique que l'opérateur doit consulter le manuel d'utilisation pour y trouver les instructions
complémentaires.
Indicates temperature
Repère température
Indicates the Over Temperature Limit system
Indique le système de dépassement de temperatur
Indicates AC Power
Repère le courant alternatif
Indicates I/ON and O/OFF
I repère de la position MARCHE de l'interrupteur d'alimentation
O repère de la position ARRÊT de l'interrupteur d'alimentation
Indicates protective earth ground
Repère terre électrique
Indicates Up and Down respectively
Touches de déplacements respectifs vers le HAUT et le BA
Indicates USB Port
Indique Port USB
GRAPHIC SYMBOLS
Each incubator is provided with multiple graphic symbols on its exterior and internal surfaces.
These symbols identify hazards and the functions of the adjustable components, as well as
important notes in the user manual.
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