Shellab SCO10W-2 User Manual

WATERJACKET CO2

INCUBATORS

WITH MICROPROCESSOR CONTROL

SCO5W SCO5W-2

SCO10W SCO10W-2

Previously designated

3517 3517-2

3524 3524-2

INSTALLATION AND OPERATION MANUAL

Sheldon Manufacturing Inc. P.O. Box 627 Cornelius, Oregon 97113

EMAIL: [e-mail] INTERNET: http://www.Shellab.com/~Shellab

1-800-322-4897 [phone] FAX [phone]

12/2013

4861607

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TABLE OF CONTENTS

SECTION 1.0 RECEIVING AND INSPECTION
SECTION 2.0 GRAPHIC SYMBOLS
SECTION 3.0 INSTALLATION
SECTION 4.0 CONTROL PANEL OVERVIEW
SECTION 5.0 OPERATION
SECTION 6.0 DATA LOGGER FUNCTION
SECTION 7.0 FYRITE CO CHECKING
SECTION 8.0 HEPA C02 FILTER
SECTION 9.0 MAINTENANCE
SECTION 10.0 TROUBLESHOOTING
SECTION 11.0 PARTS LIST

UNIT SPECIFICATIONS
SCHEMATICS

These units are CO Water Jacketed incubators for professional, industrial or educational
use where the preparation or testing of materials is done at approximately atmospheric
pressure and no flammable, volatile or combustible materials are being heated. These
units are not intended for hazardous or household locations or use.

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INTRODUCTION

Thank you for choosing a water jacket incubator. These units are not intended for use at hazardous or
household locations.
Before you use the unit, read this entire manual carefully to understand how to install, operate, and maintain
the unit in a safe manner. Your satisfaction with the unit will be maximized as you read about its safety and
operational features.

Keep this manual on-hand so it can be used by all operators of the unit. Be sure all operators of the unit are
given appropriate training before you put the unit in service.

Note: Use the unit only in the way described in this manual. Failure to follow the guidelines and

instructions in this manual may be dangerous and illegal.

General Safety Considerations

Your incubator and its recommended accessories have been designed and tested to meet strict safety
requirements.

For continued safe operation of your incubator, always follow basic safety precautions including:

 Read this entire manual before using the incubator.
 Be sure you follow any city, county, or other ordinances in your area regarding the use of this unit.
 Use only approved accessories. Do not modify system components. Any alterations or modifications to

your incubator may be dangerous and will void your warranty.

 Always plug the unit’s power cord into a grounded electrical outlet that conforms to national and local

electrical codes. If the unit is not grounded, parts such as knobs and controls may conduct electricity
and cause serious injury.

 Do not connect the unit to a power source of any other voltage or frequency beyond the range stated on

the power rating on the dataplate inside the door of the unit.

 Do not modify the power cord provided with the unit. If the plug does not fit an outlet, have a proper

outlet installed by a qualified electrician.

 Avoid damaging the power cord. Do not bend it excessively, step on it, place heavy objects on it. A

damaged cord can easily become a shock or fire hazard. Never use a power cord after it has become
damaged.

 Do not position the equipment in such a manner as to make it difficult to disconnect power cord or

coupler.

 Do not attempt to move the unit while in operation or before the unit has been allowed to cool.

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RECEIVING AND INSPECTION

Section

1

IMPORTANT: READ THIS INSTRUCTION MANUAL IMMEDIATELY!

Your satisfaction and safety require a complete understanding of this unit, including its proper
function and operational characteristics. Be sure operators are given adequate training before
attempting to put the unit in service. NOTE: This equipment must be used only for its intended
application; any alterations or modifications will void your warranty.

1.1 Inspection: The carrier, when accepting shipment, also accepts responsibility for safe delivery and is
liable for loss or damage claims. On delivery, inspect for visible exterior damage, note and describe on
the freight bill any damage found and enter your claim on the form supplied by the carrier.

1.2 Inspect for concealed loss or damage on the unit itself, both interior and exterior. If any, the carrier will
arrange for official inspection to substantiate your claim. Save the shipping crate until you are sure the
unit has been delivered in good condition.

1.3 Return Shipment: If for any reason you must return the unit, contact your customer service
representative for authorization and supply nameplate data. For information on where to contact
Customer Service please see the manual cover.

1.4 Accessories: Make sure that all of the equipment indicated on the packing slip is included with the

unit. Carefully check all packaging before discarding. Each chamber comes equipped with 3 shelves,
6 slides, 1 humidity pan and a power cord.

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GRAPHIC SYMBOLS

Section

2

Your incubator has been provided with a display of graphic symbols which is designed to
help in identifying the use and function of the available user adjustable components.

2.1 Indicates that you should consult your manual for further description or

discussion of a control or user item.

2.2 Indicates "Temperature".

2.3 Indicates "Over-temperature safety".

2.4 C Indicates "Degrees Centigrade".

2.5 CO

2.6 Indicates "Gas" (CO2 for this unit).

2.7 Indicates "AC Power ".

2.8 Indicates "Water Jacket Low".

2.9 Indicates "ON/I" and "OFF/O".

2.10 Indicates "Relative Humidity".

2.11 %RH Indicates "Percent Relative Humidity".

2.12 Indicates "Potential Shock Hazard" behind this protective partition.

2.13 Indicates "Protective Earth Ground".

Indicates "Carbon Dioxide".

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2.14 Indicates “Unit should be recycled” (Not disposed of in land-fill)

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INSTALLATION

Section

3

Local city, county, or other ordinances may govern the use of this equipment. If you have any questions about
local requirements, please contact the appropriate local agency. Installation may be performed by the end user.

Under normal circumstances this unit is intended for use indoors, at room temperatures between 5 and 40C,
at no greater than 80% Relative Humidity (at 25) and with a supply voltage that does not vary by more than
10%. The unit’s technical and environmental specifications includes: altitude not exceeding 2000 m operating
and Pollution Degree of 2. Customer service should be contacted for operating conditions outside of these
limits.

CAUTION: Make sure that the incubator is located in its intended position and level before filling the
water jacket. Directions for filling the jacket are in Section 5.2.

3.1 Power Source: The unit power requirements are listed on the data plate. PLUG THE UNIT INTO A

PROPERLY GROUNDED AND RATED RECEPTACLE OF THE CORRECT STYLE. A separate
circuit is recommended for this unit to prevent loss of product due to overloading or circuit failures
caused by other equipment. The power supply must be properly grounded (earthed) and correctly
sized to match the unit nameplate rating. The supply voltage must match the nameplate voltage
within +/- 10%. The US market units are designed for 120 volts @ 60 Hz, 5 Amps. The EU market
units are designed for 230 volts @ 50 Hz, 3.5 Amps. If supplied with a detachable cord set, plug the
female end into the inlet on the unit and the male plug into the supply. Assure that units requiring fuses
have them installed. The fuse is a T type, 10 amp for 120 vol units, 6.3 amp for 230 volt units. This
fuse may be at the inlet or a part of the cord set male plug. The unit must be located to easily allow for
it to be unplugged or disconnected.

3.2 Location: When selecting a site for the unit, consider conditions which may affect performance, such

3.3 Lifting / Handling: These units are heavy and care should be taken to use appropriate lifting devices

3.4 Leveling: The unit must sit level and solidly. Leveling feet are supplied and must be installed in the

3.5 Cleaning: The unit chamber should be cleaned and disinfected prior to use. The operating

as heat from steam radiators, ovens, autoclaves, etc. Avoid direct sun, fast-moving air currents,
heating/cooling ducts, and high-traffic areas. To ensure air circulation around the unit, allow a
minimum of 10cm of clearance between the incubator and surrounding walls, or obstructions to free air
flow.

that are sufficiently rated for these loads. Units should only be lifted from their bottom surfaces.
Doors, handles and knobs are not adequate for lifting or stabilization. The unit should be completely
restrained from tipping during lifting or transport. All moving parts, such as shelves and trays should
be removed and doors need to be positively locked in the closed position during transfer to prevent
shifting and damage.

four holes in the bottom corners of the unit. With the feet installed and the unit standing upright, each
foot can be raised by turning it in a counterclockwise direction. Adjust the foot at each corner until the
unit stands level and solid without rocking. If the unit must be moved, drain all of the water from the unit
and turn the leveling feet in (clockwise) all the way to prevent damage while moving.

conditions and appropriate protocol will determine the correct procedure for decontamination. A
typical decontamination procedure that is adequate for many situations has been described below.
As well, certain steps are listed that will help reduce the likelihood of contamination and the
necessity of decontamination. Whatever process is appropriate, it needs to be done on a regularly
scheduled basis. Depending on usage and protocol, this may be monthly, quarterly or otherwise.

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Regardless of the decontamination procedure used, certain precautions will need to be taken:
A. Always disconnect the unit from the electrical service when cleaning. Assure all volatile or

flammable cleaners are evaporated and dry before reconnecting the unit to the power
supply.

B. Special care should be taken when cleaning around sensing heads to prevent damage.
C. If you use chlorine-based bleaches or abrasive cleaners this will modify the stainless steel

interior finish. DO NOT USE hard tools such as metal wire brushes or steel wool. Use non­abrasive cleaners and soft tools such as plastic brushes.

D. In the event hazardous material is spilled onto or into the equipment, appropriate

decontamination must be carried out. If there is any doubt about the compatibility of
decontamination or cleaning agents with parts of the equipment or with material contained,
please contact the manufacturer or his agent. No decontamination or cleaning agents
should be used which could cause a hazard as a result of a reaction with parts of the
equipment or with the material contained in it.

TYPICAL DECONTAMINATION PROCEDURE

1. Remove the humidity pan every week and autoclave, or wash with soap and water then

disinfect with 70% alcohol solution. Replace in the incubator with fresh, DISTILLED water.

2. Flush the sample port tubing with 70% alcohol solution. Replace any lines that have a

green color.

3. Remove the door gaskets, clean and disinfect. Clean and disinfect all mounting grooves for

the door gaskets.

4. Remove all shelves, shelf supports, shelf standards and shields. Autoclave, or wash and

disinfect as described in item 1.

5. Wash and disinfect all interior surfaces.

6. Give special attention to cleaning and disinfecting all access ports, air bleeds, shaft holes,

electrical pass-through and any other passages into the chamber.

7. Replace all air and CO2 filters every six months or when noticeably dirty on the upstream

side. CO2 filters are located in the shadow box just behind the GAS IN fitting and in line with
the CO2 tubing kit. Units with flow meters require operator- supplied air pumps. These
pumps should be checked for proper air filtering.

OPERATION FOR MINIMIZING CONTAMINATION

1. Keep the outside of the incubator, including the air in the laboratory, as clean as possible.

This is particularly important for units placed directly on the floor. Do not place incubators
near doors, air vents or other areas of high air movement or traffic.

2. The floor around the unit needs to be clean. Units that are placed on the floor should be

mounted higher – typically on a caster platform – for ease of moving the unit during cleaning
and access to the back of the unit.

3. Minimize the number of times access is made to the chamber during normal operation.

4. Do not depend on the use of antibiotics to maintain uncontaminated conditions, as this is an

inadequate technique for sterilization. Preferably use aseptic techniques as described
above for maintaining sterile conditions in the incubator.

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To use your SHEL LAB CO2 Incubator USB features, you need
to download the software by clicking on the image below.

3.6 Shelves and Interior Parts: Shelving, shelf supports and a humidity pan are supplied with
each unit. See Figure 1, for proper placement of these accessories.

Figure 1

3.7 Download USB Software: Go to www.shellab.com website home page and scroll down

towards the bottom of the page. Click on this section:

Open the Excel Spreadsheet SLDL0092 and download information

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CONTROL PANEL OVERVIEW

Section

4

All controls are located on the front panel. Units with a detachable cord have a fused inlet located at the top
rear of the control panel. This inlet has a recessed male plug, fuse and an EMI filtering system designed to
filter out electrical interference. This inlet also prevents any internally generated interference from feeding out
to the power grid.

4.1 Power Switch: The I/O (ON/OFF) switch controls all of the power for the incubator and must be in the
I/ON position before any systems are operational. Both temperature and CO2 displays will illuminate
when the power switch is in the ON position.

4.2 Water Low Light: This pilot lamp is on whenever the internal water jacket float switch has been
tripped to the closed position. When the water drops low enough the float switch closes the circuit
turning on the indicator light.

4.3 Over Temperature Light: This pilot lamp is on whenever the Over Temperature Safety thermostat

4.4 Main Temperature Control: This controller is marked in C and indicates the actual temperature

4.5 Heating Light: This pilot lamp is on whenever the Temperature Controller is activating the heating

4.6 C02 Control On Water Jacket Incubators: The control sets the rate and the limit of injection

has been activated and taken control of the element. During normal operating conditions this indicator
should never be on.

within the chamber to .1C. The UP/DOWN buttons are used for imputing the set point, calibrating the
display, and muting or unmuting the audible alarm. The HIGH and LOW alarm indicators will light
whenever there is an alarm condition associated with the temperature within the chamber. The MUTE
indicator will light whenever the audible alarm has been deactivated. Some alarm conditions have a
delay programmed in.

element.

of CO2 into the incubator based on input value and some variables. The input value is set by the
operator. This is set at 5% CO2 in production.

At start-up, the incubator must be warmed up to operating temperature. This is set in production at
37°C. During warm up, the water tray for humidity should be filled and placed inside. This allows
the humidity to reach 90-95% before CO2 is injected.

The CO2 should be turned OFF during warm up. Failure to do so will cause the control overshoot
the set value input by the operator, the injection value can be reset by setting the CO2 levels down
until CL shows on the display. Then resetting the CO2 control to the desired setting resets the