SECTION 1.0 RECEIVING AND INSPECTION
SECTION 2.0 INSTALLATION
SECTION 3.0 GRAPHIC SYMBOL
SECTION 4.0 CONTROLS OVERVIEW
SECTION 5.0 OPERATION
SECTION 6.0 CALABRATION AND READING A FYRITE
SECTION 7.0 MAINTENANCE
SECTION 8.0 HEPA C02 FILTER
SECTION 8.0 TROUBLESHOOTING
SECTION 9.0 PARTS LIST
UNIT SPECIFICATIONS
SCHEMATICS
These units are TUV CUE listed as air jacketed CO2 incubators for professional,
industrial, or educational use where the preparation or testing of materials is done at
approximately atmospheric pressure and no flammable, volatile, or combustible
materials are being heated.
These units have been tested to the following requirements:
CAN/CSA C22.2 No. 61010-1:2012
CAN/CSA C22.2 No. 61010-2-010 + R:2009
UL 61010-1:2004 + R:2005-07 + R:2008-10
UL 61010A-2-010:2002
UL 61010-1:2012
EN 61010-1:2010
EN 61010-2-010:2003
IEC 61010-1:2010
IEC 61010-2-010:2003
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RECEIVING AND INSPECTION
Section
Your satisfaction and safety require a complete understanding of this unit. Read the instructions
thoroughly and be sure all operators are given adequate training before attempting to put the
unit in use. NOTE: This equipment must be used only for its intended application; any
alterations or modifications will void your warranty.
1.1 Inspection: The carrier, when accepting shipment, also accepts responsibility for safe delivery and
is liable for loss or damage. On delivery, inspect for visible exterior damage, note and describe on
the freight bill any damage found, and enter your claim on the form supplied by the carrier.
1.2 Inspect for concealed loss or damage on the unit itself, both interior and exterior. If any, the carrier
will arrange for official inspection to substantiate your claim.
1.3 Return Shipment: Save the shipping crate until you are sure all is well. Never ship anything back
inside the chamber. If for any reason you must return the unit, contact your service representative
for authorization and supply the data plate information. Please see the manual cover for
information on where to contact Customer Service.
1.4Accessories: Verify that your accessory kit is complete. The SCO5A (5215) and SCO5A-2 and
(5215-2) are equipped with 3 shelves, supply hose kit, 4 leveling feet and pan. The SCO10A
(5230) and SCO10A-2 (5230-2) are equipped with 6 shelves, supply hose kit, 4 leveling feet and 2
pans. Check all packaging materials before discarding.
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INSTALLATION
Section
Local city, county, or other ordinances may govern the use of this equipment. If you have any questions
about local requirements, please contact the appropriate local agency. Installation may be performed by the
end user.
Under normal circumstances this unit is intended for use indoors, at room temperatures between 5 and
35C, at no greater than 80% Relative Humidity (at 25) and with a supply voltage that does not vary by more than 10%. The unit’s technical and environmental specifications includes: altitud e not exceeding
2000 m operating and Pollution Degree of 2. Customer service should be contacted for operating
conditions outside of these limits.
2.1 Power Source: The unit power requirements are listed on the data plate. PLUG THE UNIT INTO A
2.2 Location: In selecting a location, consider all conditions, which might affect performance, such as
2.3 Lifting / Handling: These units are heavy and care should be taken to use appropriate lifting
2.4 Leveling: The unit must sit level and solidly. Leveling feet are supplied and must be installed in
2.5 Cleaning: The unit chamber was cleaned with a decontaminant at the factory, but it should be
PROPERLY GROUNDED AND RATED RECEPTACLE OF THE CORRECT STYLE. A separate
circuit is recommended for this unit to prevent loss of product due to overloading or circuit failures
caused by other equipment. The power supply must be properly grounded (earthed) and correctly
sized to match the unit nameplate rating. The supply voltage must match the nameplate voltage within +/- 10%. The US market units are designed for 110 volts @ 50/60 Hz, 6.0 Amps. The EU
market units are designed for 220 volts @ 50/60 Hz, 3.0 Amps. If supplied with a detachable cord
set, plug the female end into the inlet on the unit and the male plug into the supply. Assure that
units requiring fuses have them installed. The fuse is a T type, 10 Amp for 110 vol units, 6.3 Amp
for 220 volt units. This fuse may be at the inlet or a part of the cord set male plug. The unit must be
located to easily allow for it to be unplugged or disconnected.
heat from radiators, ovens, autoclaves, etc. Avoid direct sun, fast-moving air currents,
heating/cooling ducts and high-traffic areas. To ensure air circulation around the unit, allow a
minimum of 10cm (4”) between the unit and walls or partitions which might obstruct free airflow.
devices that are sufficiently rated for these loads. Units should only be lifted from their bottom
surfaces. Doors, handles and knobs are not adequate for lifting or stabilization. The units should
be completely restrained from tipping during lifting and transport. All moving parts, such as shelves
and trays should be removed and doors need to be positively locked in the closed position during
transfer to prevent shifting and damage.
the four holes in the bottom corners of the unit. With the feet installed and the unit standing upright,
each foot can be raised by turning it in a counterclockwise direction. Adjust the foot at each corner
until the unit stands level and solid without rocking. If the unit is to be moved, turn the leveling feet
all the way clockwise to prevent damage while moving.
cleaned and disinfected prior to use. Operating conditions and appropriate protocol will
determine the correct procedure for decontamination. A typical decontamination procedure that
is adequate for many situations is described below, as are certain steps that will help reduce the
likelihood of contamination and the necessity of decontamination. Whatever process is
appropriate, it needs to be done on a regularly scheduled basis. Depending on usage and
protocol, this may be monthly, quarterly or otherwise. Regardless of the decontamination
procedure used, certain precautions will need to be taken:
A. Always disconnect the unit from the electrical service when cleaning.
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Assure all volatile or flammable cleaners are evaporated and dry before reconnecting the unit
to the power supply.
B. Special care should be taken when cleaning around sensing heads to Prevent damage. (See
figure 1)
C. Do not use chlorine-based bleaches or abrasive cleaners. These will modify the
stainless steel interior finish. DO NOT USE hard tools such as metal wire brushes or
steel wool. Use non-abrasive cleaners and soft tools such as plastic brushes. (See
7.1–A)
D. Use only DISTILLED water in the humidity pan. DO NOT USE de-ionized water due to its
reactivity with metal components.
TYPICAL DECONTAMINATION PROCEDURE
1. Remove the humidity pan every week and autoclave, or wash with soap and water then
disinfect with 70% alcohol solution diluted with distilled water. Replace in the incubator with
fresh, DISTILLED water.
2. Flush the sample port tubing with 70% alcohol solution. Replace any discolored lines.
3. Clean and disinfect the liner gaskets. Clean and disinfect all mounting grooves for the door
gaskets.
4. Remove all shelves, shelf supports, shelf standards and slides. Autoclave, or wash and
disinfect as described in item 1.
5. Wash and disinfect all interior surfaces.
6. Give special attention to cleaning and disinfecting all access ports, shaft holes, electrical
pass-through, and any other passages into the chamber.
7. Replace all HEPA (Patent No. 6,333,004) and CO2 filters every six months or when
noticeably dirty on the upstream side. CO2 filters are located in the shadow box just behind
the GAS IN fitting and on CO2 sample line. Hepa filter is located on the top air duct inside the
chamber.
OPERATION FOR MINIMIZING CONTAMINATION
1. Keep the outside of the incubator, including the air in the laboratory, as clean as possible.
This is particularly important for units placed directly on the floor. Do not place incubators
near doors, air vents or other areas of high air movement or traffic.
2. The floor around the unit needs to be clean. Units that are placed on the floor should be
mounted higher – typically on a caster platform – for ease of moving the unit during cleaning
and access to the back of the unit.
3. Minimize the number of times access is made to the chamber during normal operation.
4. Do not depend on the use of antibiotics to maintain uncontaminated conditions, as this is an
inadequate technique for sterilization. Preferably use aseptic techniques as described above
for maintaining sterile conditions in the incubator.
Figure 1
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2.6 Place shelves in chamber as desired.
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GRAPHIC SYMBOLS
Section
Your CO2 incubator is provided with a display of graphic symbols on the control panel,
which are designed to help identify the use and function of the adjustable components.
1. Indicates that you should consult your manual for further
description and discussion of a control or user item.
2. Indicates “Temperature”
3. Indicates “Over Temperature”
4. C Indicates “Degrees Centigrade”
5. Indicates “AC Power”
6. Indicates “Manual Adjustment”
7. Indicates “Gas” (CO2 pressurized gas for this unit)