Shel Lab SCO10A User Manual
Installation and
Operation Manual
SCO10A SCO5A
Previously Designated:
5230 5215
AIR-JACKETED CO2 INCUBATOR 110 - 120 Voltage
Page left blank
2 | P a g e
SCO5A SCO10A AIR-JACKETED CO2 INCUBATORS
Installation and Operation Manual
Part Number (Manual): 4861730
Revision: March 22, 2016
Note: The SCO10A consists of two stacked and bolted together SCO5A incubators. These
incubators operate independently of one another.
These units are TÜV CUE listed as air-jacketed CO2 Incubators for professional, industrial, or
educational use where the preparation or testing of materials is done at an ambient air pressure
range of 22.14 – 31.3 inHg (75 – 106 kPa) and no flammable, volatile, or combustible materials are
being heated.
The units were tested to the following standards:
CAN/CSA C22.2 No. 61010-1:2012
CAN/CSA C22.2 No. 61010-2-010:2004 Reaffirmed: 2014-07
UL 61010-1:2012-05
UL 61010A-2-010:2002-03
EN 61010-1:2010
EN 61010-2-010:2014
Supplemented by: UL 61010-2-010:2015
3 | P a g e
TABLE OF CONTENTS
INTRODUCTION ........................................................................................................................................... 6
General Safety Considerations ................................................................................................................. 6
Engineering Improvements ....................................................................................................................... 7
Contacting Assistance ............................................................................................................................... 7
RECEIVING YOUR INCUBATOR ................................................................................................................ 8
Inspect the Shipment ................................................................................................................................ 8
Regulator ................................................................................................................................................... 9
Orientation Photos................................................................................................................................... 10
Recording Data Plate Information ........................................................................................................... 12
Reference Sensor Devices ..................................................................................................................... 12
INSTALLATION .......................................................................................................................................... 13
Installation Checklist ............................................................................................................................... 13
Required Ambient Conditions ................................................................................................................. 14
Environmental Disruption Sources .......................................................................................................... 14
Power Source Requirements .................................................................................................................. 14
Lifting and Handling ................................................................................................................................ 15
Install Incubator in Location .................................................................................................................... 15
Leveling ................................................................................................................................................... 15
Deionized and Distilled Water ................................................................................................................. 15
Installation - Clean and Disinfect ............................................................................................................. 16
Install Chamber HEPA Filter and Duct .................................................................................................... 16
Shelving Installation ................................................................................................................................ 17
Connect to the CO2 Supply ..................................................................................................................... 18
Access Port Stopper ............................................................................................................................... 19
GRAPHIC SYMBOLS ................................................................................................................................. 20
CONTROL PANEL OVERVIEW ................................................................................................................. 22
OPERATION ............................................................................................................................................... 24
Theory of Operation ................................................................................................................................ 24
Put the Incubator into Operation ............................................................................................................. 26
Humidify the Incubator ............................................................................................................................ 27
Set the Temperature Set Point ................................................................................................................ 28
Muting the Audible Temperature Alarm .................................................................................................. 29
Automatic Door Cutoff ............................................................................................................................. 29
Set the CO2 Set Point .............................................................................................................................. 30
Muting the Audible CO2 Alarm ................................................................................................................ 31
No Gas Supply Alarm (NGS) .................................................................................................................. 31
Temperature Accuracy Verification ......................................................................................................... 32
CO2 Accuracy Verification ....................................................................................................................... 34
Set the Over Temperature Limit .............................................................................................................. 36
Load the Incubator .................................................................................................................................. 37
Accessory Compatibility .......................................................................................................................... 37
Data Output Capabilities ......................................................................................................................... 37
Condensation and the Dew Point ............................................................................................................ 39
USER MAINTENANCE ............................................................................................................................... 40
Cleaning and Disinfecting ....................................................................................................................... 40
Minimizing Contamination Exposure ....................................................................................................... 41
Gas Lines and HEPA Filters ................................................................................................................... 42
Storage of the Incubator .......................................................................................................................... 42
Maintaining Atmospheric Integrity ........................................................................................................... 42
Electrical Components ............................................................................................................................ 42
4 | P a g e
Replace the Chamber HEPA Filter ......................................................................................................... 43
Calibrate the Temperature display .......................................................................................................... 44
Calibrate the CO2 Display ........................................................................................................................ 48
UNIT SPECIFICATIONS ............................................................................................................................. 52
Weight ..................................................................................................................................................... 52
Dimensions .............................................................................................................................................. 52
Capacity .................................................................................................................................................. 53
CO2 .......................................................................................................................................................... 53
Temperature ............................................................................................................................................ 53
Power ...................................................................................................................................................... 53
Pressure Conversion Chart ..................................................................................................................... 53
PARTS LIST ............................................................................................................................................... 54
Ordering Parts and Consumables ........................................................................................................... 54
5 | P a g e
INTRODUCTION
Thank you for purchasing a Shel Lab product. We know that in today’s competitive marketplace
customers have many choices when it comes to constant temperature equipment. We appreciate
you choosing ours. Our continued reputation as a leading laboratory product manufacturer rests
with you. We stand behind our products and will be here for you if you need us.
These incubators are intended for laboratory, industrial, and educational microbiological cultivation
applications. These incubators are not intended for use in hazardous or household locations.
Before you use the unit read this manual in its entirety to understand how to install, operate, and
maintain the incubator in a safe manner. Keep this manual available for use by all operators.
Ensure that all operators are given appropriate training before the incubator begins service.
GENERAL SAFETY CONSIDERATIONS
Note: Failure to follow the guidelines and instructions in this manual may create a protection
impairment by disabling or interfering with the unit safety features. This can result in injury or
death.
Your unit and its recommended accessories are designed and tested to meet strict safety
requirements. It is designed to connect to a power source using the specific power cord type
shipped with the unit.
For continued safe operation of your incubator, always follow basic safety precautions including:
Always plug the incubator power cord into a protective earth grounded electrical receptacle
(outlet) that conforms to national and local electrical codes. If the incubator is not grounded
properly, parts such as knobs and controls can conduct electricity and cause serious injury.
Do not bend the power cord excessively, step on it, or place heavy objects on it.
A damaged cord can be a shock or fire hazard. Never use a power cord if it is damaged or
altered in any way.
Always position the unit so that the user can quickly unplug it in the event of an emergency.
Do not attempt to move the unit while in operation or before the unit has cooled.
Do not stack the unit without a factory-approved stacking rack or adaptor.
Use only approved accessories. Do not modify system components. Any alterations or
modifications to your incubator can be dangerous and void your warranty.
Follow all local ordinances in your area regarding the use of this incubator. If you have any
questions about local requirements, please contact the appropriate agencies.
6 | P a g e
INTRODUCTION (CONTINUED)
ENGINEERING IMPROVEMENTS
Sheldon Manufacturing continually improves all of its products. As a result, engineering changes
and improvements are made from time to time. Therefore, some changes, modifications, and
improvements may not be covered in this manual. If your unit operating characteristics or
appearance differs from those described in this manual, please contact your Shel Lab dealer or
distributor for assistance.
CONTACTING ASSISTANCE
If you are unable to resolve a technical issue with the incubator, please contact Sheldon Technical
Support. Phone hours for Technical Support are 6am – 4:30pm Pacific Coast Time (west coast of
the United States, UTC -8). Please have the following information ready when calling or emailing:
the model number and the serial number (see page 11).
EMAIL: tech@shellab.com PHONE: 1-800-322-4897 extension 4, or (503) 640-3000 FAX: (503)
640-1366
Sheldon Manufacturing INC.
P.O. Box 627
Cornelius, OR 97113
USA
7 | P a g e
1 Copper Pan Token
1 Access Port
Stopper*
1 Ceiling Air Duct
1 Chamber HEPA
Filter
1 Chamber Filter Cap
5800529
7750514
5121527
2800517
6500606
1 CO2 Tubing Kit
CO2 HEPA Filter
1 Humidification
Pan
4 Leveling Feet
Power Cord
9710500
2800525
995-00015
2700512
1800510
6 Shelf Slides
4 Stainless Steel
Shelf Standards
3 Shelves
5121026
5170646
5121525
RECEIVING YOUR INCUBATOR
INSPECT THE SHIPMENT
When a unit leaves the factory, safe delivery becomes the responsibility of the carrier.
Damage sustained during transit is not covered by the manufacturing defect warranty.
When you receive your unit, inspect it for concealed loss or damage to its interior and exterior. If
you find any damage to the unit, follow the carrier’s procedure for claiming damage or loss.
1. Carefully inspect the shipping carton for damage.
2. Report any damage to the carrier service that delivered the unit.
3. If the carton is not damaged, open the carton and remove the contents.
4. The unit should come with an Installation and Operation Manual.
5. Verify that the correct number of accessories have been included.
Included Accessories SCO5A
8 | P a g e
2 Copper Pan Tokens
2 Access Port
Stoppers*
2 Ceiling Air Ducts
2 Chamber HEPA
Filters
2 Chamber Filter Caps
5800529
7750514
5121527
2800517
6500606
2 CO2 Tubing Kits
2 CO2 HEPA Filters
2 Humidification
Pans
8 Leveling Feet
2 Power Cords
9710500
2800525
995-00015
2700512
1800510
12 Shelf Slides
8 Stainless Steel
Shelf Standards
6 Shelves
5121028
5170646
5121525
Figure 1: CO2
Regulator
(9740558)
RECEIVING (CONTINUED)
Included Accessories SCO10A
REGULATOR
The incubator must be connected to either a building CO2 gas supply system or a supply
cylinder (tank). A cylinder regulator is not included with the incubator, and must be purchased
separately. Please see the Accessories section on page 55 if you wish to order one from Shel
Lab.
9 | P a g e
Figure 2:
SCO10A
Top Incubator
Control Panel
Top
Incubation
Chamber
Bottom Incubation
Chamber Door
Door Gasket
Chamber
Door
Handle
Bottom Viewing
Door (Glass)
Chamber
HEPA Filter
Door Latch
Bottom
Incubator
Shelf Standard
Mounting Rail
Back of Unit
(Top Incubator)
CO2 In Port
Top Viewing
Door (Glass)
Back of Unit
(Bottom
Incubator)
Fuse in Power Inlet
USB-Style Port
CO2 Sample Port
(Side of Unit)
Viewing Door
Knob
Humidification
Pan
Top Incubation
Chamber Door
Access Port (In
Chamber)
Ceiling Air
Duct
Chamber
Gasket
USB-Style
Port
CO2 In Port
Fuse in Power Inlet
Access Port
(In Chamber)
Top Chamber
Data Plate
Bottom
Chamber
Data Plate
CO2 Sample Port
(Side of Unit)
Second Fuse
in Adjacent
Fuse Holder
RECEIVING (CONTINUED)
ORIENTATION PHOTOS
10 | P a g e
Figure 3: SCO5A
Back of Unit
CO
2
In Port
Fuse in Power
Inlet
USB-Style Port
Control Panel
CO2 Sample Port
(Side of Unit)
Chamber
Gasket
Ceiling Air
Duct
Chamber
HEPA Filter
Door Latch
Humidification Pan
Viewing Door (Glass)
Chamber Door
Chamber Door Handle
Viewing Door Knob
Access Port
(Side of Unit)
Door Gasket
RECEIVING (CONTINUED)
11 | P a g e
Model Number
Serial Number
RECEIVING (CONTINUED)
RECORDING DATA PLATE INFORMATION
Locate the data plate on the back of the bottom incubator unit, adjacent to the power cord inlet. The
data plate contains the incubator model and serial numbers. Enter this information below for future
reference.
The SCO10A is also provided with individual SCO5A incubator unit data plates on the top right
corner of each incubation chamber door interior. You do not need to record the SCO5A door data
plate information here.
Date Plate Information
REFERENCE SENSOR DEVICES
Reference sensor devices or a combined device must be purchased separately in order to perform
accuracy verifications and calibrations of the incubator temperature and CO2 displays.
Reference devices must be accurate to at least 0.1°C and 0.1% CO2. The devices should be
regularly calibrated, preferably by a third party. For best temperature results, use a digital device
with a wired-connected temperature sensing probe that can be placed in the incubation chamber
through the unit access port. For example a wire thermocouple probe. For best CO2 accuracy, use
a calibrated digital gas analyzer with sample tubing that can be connected to the incubator external
CO2 sample port.
Reference readings that avoid chamber door openings during verification and calibration eliminate
subsequent waits for the chamber temperature and gas levels to re-stabilize before proceeding.
This also allows temperature and gas verifications or calibrations to be performed simultaneously.
Select probes suitable for the application temperature you will be calibrating or verifying the
incubator displays at.
Alcohol thermometers are insufficient for conducting accurate temperature verifications and
calibrations. Do not use a mercury thermometer. Never place a mercury thermometer in the
incubation chamber.
12 | P a g e
INSTALLATION
INSTALLATION CHECKLIST
Carry out the steps and procedures listed below to install the unit in a new workspace location and
prepare it for use. All procedures are found in the Installation section of this manual.
Pre-Installation
Procure a CO
Always use medical grade CO2.
gas supply for the incubator with a concentration suitable for your application.
2
Check that the required ambient conditions, ventilation, and spacing for the incubator are met,
page 14.
Unit dimensions may be found on page 52
Check for performance-disrupting heat and cold sources in the environment, page 14
Check that a suitable electrical outlet and power supply is present, page 14
Install the Incubator in a suitable location
Review the lifting and handling instructions, page 15
Install the incubator in its workspace location, page 15
Make sure the incubator is level, page 15
Set up the Incubator for use
Clean and disinfect the incubator and accessories that will be placed in the incubation
chamber, page 16
Do not use deionized water to clean the unit, see page 15
Install the shelving in the incubation chamber, page 17
Connect the incubator to the CO
gas supply source, page 18
2
Verify the port stopper is installed in the access port inside the incubation chamber, page 19
13 | P a g e
INSTALLATION (CONTINUED)
REQUIRED AMBIENT CONDITIONS
SCO incubators are intended for use indoors at room temperatures between 15C and 30C (59F
and 86F), at no greater than an ambient 80% Relative Humidity (at 25C / 77F).
4 inches (10cm) minimum ventilation clearance between the incubator and walls or partitions.
2 inches (5cm) minimum clearance above the top of the incubator for unobstructed airflow.
Operating the unit outside these conditions may adversely affect its temperature range and
stability. For conditions outside of those listed above, please contact your distributor to explore
other unit options suited to your laboratory or production environment.
ENVIRONMENTAL DISRUPTION SOURCES
When selecting a location to install the incubator, consider all environmental conditions that can
affect the unit temperature performance. For example:
Proximity to ovens, autoclaves, and any device that produces significant radiant heat
Heating and cooling ducts, or other sources of fast-moving air currents
High-traffic areas
Direct sunlight
POWER SOURCE REQUIREMENTS
When selecting a location for the unit, verify that each of the following requirements are satisfied.
Wall power sources must match the power requirements listed on the unit data plate. These units
are intended for 110 – 120 VAC 50/60 Hz applications at 6.0 amps.
Wall power sources must be protective earth grounded and conform to all national and
local electrical codes.
Supplied voltage must not vary more than 10% from the data plate rating. Damage to
the unit may result if supplied voltage varies more than 10%.
Use a separate circuit to prevent loss of product due to overloading or circuit failure. The
circuit must match or exceed the amperage requirement listed on the unit the data plate.
The unit must be positioned so that all end-users can quickly unplug power cords in the event of an
emergency.
The unit is provided with a 115 volt 15 Amp, 9ft 5 in (2.86m) NEMA 5-15P power cord for
each inlet (SCO5A 1 cord, SCO10A 2 cords).
The unit is provided with a 250V time-lag T 10 amp 5x20mm fuse located in each power
inlet.
14 | P a g e
Figure 4:
Leveling Foot
INSTALLATION (CONTINUED)
LIFTING AND HANDLING
The unit is heavy. Use appropriate powered lifting devices. Follow these guidelines when lifting and
handling the unit:
Lift the unit only from its bottom surface.
Doors, handles, and knobs are not adequate for lifting or stabilization.
Restrain the unit completely while lifting or transporting so it cannot tip.
Remove all removable components, such as shelving, and secure all doors in the closed
position during transfer to prevent shifting and damage.
INSTALL INCUBATOR IN LOCATION
Install the unit in a workspace location that meets the criteria discussed in the previous entries of
the Installation section.
LEVELING
Install the leveling feet shipped with the unit. The unit must be level and stable for safe
operation.
Insert one leveling foot into each of the four holes in the bottom corners of the unit.
Adjust the foot at each corner until the unit stands level and solid without rocking. To
raise a foot, turn it in a counterclockwise direction.
To lower a foot, turn it in a clockwise direction.
Note: To prevent damage when moving the unit, turn each of the four leveling feet completely
clockwise.
DEIONIZED AND DISTILLED WATER
Do not use deionized water to clean or humidify the incubator, or fill the water jacket. Use of
deionized water may corrode metal surfaces and voids the warranty. The manufacturer
recommends the use of distilled water in the resistance range of 50K Ohm/cm to 1M Ohm/cm, or a
conductivity range of 20.0 uS/cm to 1.0 uS/cm, for cleaning, humidifying, and water-jacketing
applications.
15 | P a g e
Front
Chamber
Ceiling Air
Duct
Step 1
Chamber
HEPA Filter
Filter Cap
Back
Step 2
Step 3
Step 5
Front
Front Mounting Pins
Step 4
Back
Rear Mounting Pins
INSTALLATION (CONTINUED)
INSTALLATION - CLEAN AND DISINFECT
Cleaning and disinfecting the unit incubation chamber, shelving
components, and ceiling air duct now reduces the risk of
contamination. The chamber and shelving were cleaned and
disinfected at the factory, however, the unit may have been exposed
to contaminants during shipping.
Remove all protective wrappings from shelving components
and the ceiling air duct prior to cleaning.
Do not clean the chamber HEPA filter!
See the Cleaning and Disinfecting entry on page 40 for
information on how to clean and disinfect without damaging
the incubator or its components.
INSTALL CHAMBER HEPA FILTER AND DUCT
Note: Exercise caution to avoid striking the sensors and blower fan
wheel on the chamber ceiling when installing the duct.
Note: The incubator must be turned off and unplugged when carrying
out this procedure.
The ring-style chamber HEPA filter traps particulates, as well as
isolating and killing airborne microbes in the incubation chamber.
Perform the following steps to install the filter and ceiling air duct.
Snap the chamber HEPA filter to the mounting collar on the
chamber ceiling air duct.
a. It may be necessary to tilt the filter to one side or the
other.
Snap the filter cap to the HEPA filter.
Move the air duct into the incubation chamber with the
attached HEPA filter facing down.
Mount the back of the air duct on the rear chamber mounting
pins, one pin at a time.
16 | P a g e
Mount the front of the duct on the front chamber mountings,
one at a time.
Figure 5: Shelving Installation
INSTALLATION (CONTINUED)
SHELVING INSTALLATION
Note: Always install the copper token in the humidification pan. Copper is known to have
antimicrobial properties that retard the growth of microorganisms in the pan.
Install the shelving and humidification pan in the in the
incubation chamber.
Install the shelf standard rails.
a. Align the keyhole slot of the standard with the
mounting peg on the side of the chamber wall.
b. Mount the shelf standard.
Install the shelf slides.
a. Insert the shelf slide into the shelf standard
using a rocking motion.
b. The shelf slide will sit level when correctly
installed.
Install the shelves.
a. Slide into position.
Install the humidification pan.
a. Place the copper token in the humidity pan.
b. Secure the token using the clip on the bottom
of the pan.
c. Place the pan on the chamber floor.
17 | P a g e
Loading...