Shellab BACTROX User Manual

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BACTROX HYPOXIC CHAMBERS

OPERATION MANUAL

MODEL: BACTROX

Sheldon Manufacturing Inc. P.O. Box 627 Cornelius, Oregon 97113

EMAIL: [e-mail] INTERNET: http://www.Shellab.com

Rev 8/ 25/2014

4861671

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Contents

OPERATION MANUAL .................................................................................................. 1

1. ORIENTATION ............................................................................................................... 3

2. RECEIVING AND INSPECTION .................................................................................... 6

3. INSTALLATION .............................................................................................................. 6

4. CONTROL PANEL OVERVIEW ..................................................................................... 8

5. HOSE AND ELECTRICAL CONNECTIONS .................................................................. 8

6. THERMOELECTRIC CONDENSATE CONTROLER .................................................... 9

7. INCUBATOR TEMPERATURE CONTROLLER/SET OVER TEMPERATURE ............ 9

8. CO2 CONTROL CALIBRATION .................................................................................. 10

9. OXYGEN CONTROL CALIBRATION .......................................................................... 11

10. POWER ON THE BACTROX: ...................................................................................... 13

11. MAKING THE CHAMBER HYPOXIC ........................................................................... 14

12. PASS BOX OPERATION ............................................................................................. 14

13. ENTERING THE CHAMBER ........................................................................................ 15

14. EXITING THE CHAMBER ............................................................................................ 15

15. MAINTENANCE ........................................................................................................... 15

16. ACCESSORIES ............................................................................................................ 16

17. TROUBLESHOOTING ................................................................................................. 17

18. BACTROX OPERATION TIPS ..................................................................................... 19

19. UNIT SPECIFICATIONS .............................................................................................. 20

20. DIAGRAMS .................................................................................................................. 21

21. PLUMBING DIAGRAM ................................................................................................. 31

22. BACTROX SPARE PARTS LIST ................................................................................. 32

Bactrox Operations Manual 2

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Document/Chart

holder

Pass Box

GAS

Connections

Incubator

Arm Port Door

Storage

Arm Ports

With Sleeve

Control Panel

Oxygen

Sensor &

Box

IMPORTANT: READ THIS INSTRUCTION MANUAL IMMEDIATELY.

Your satisfaction and safety require a complete understanding of this unit, including its proper function and
operational characteristics. Be sure operators are given adequate training before attempting to put the unit in
service. NOTE: This equipment must be used only for its intended application; any alterations or

modifications will void your warranty.

1. ORIENTATION

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Replaceable Arm Port

door O-ring seals.

Figure 1: Sleeve/Cuff Assembly

The Sleeve/Cuff Assembly consists of a neoprene sleeve, a plastic cuff ring, O-Ring, and a soft rubber cuff. Unit
comes with Latex Gloves and Cuffs. Nitrile Gloves and Cuffs may be ordered from the Parts List. The assembly
allows gloveless chamber operation.

Figure 2: Arm port Doors

CAUTION: The Bactrox chambers have an efficient method of sealing the chamber by utilizing the Arm port

doors as shown in Figure 3. The tightening knob on the Arm port doors should be turned just until the knob
begins to “grab”. DO NOT OVER TIGHTEN. Damage can occur if too much force is placed on the Arm port door
assembly.

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Inner Airlock Door

Oxygen

Sensor and

Box

Incubator

Figure 5. Pass Box

The PASS BOX provides ability to take material into and out of the chamber. PASS BOX can be operated either
automatically or manually. The PASS BOX shelf allows for materials to be conveniently rolled from the PASS
BOX into the chamber.

Figure 6: Incubator Bottom Shelf Spacer

Three (3) are provided with the Bactrox. Refer to Section 8 for installation and precautions.

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2. RECEIVING AND INSPECTION

2.1 The carrier, when accepting shipment, also accepts responsibility for safe delivery and is liable

for loss or damage claims. On delivery, you must inspect for visible exterior damage. Note and
describe on the freight bill any damage found and enter your claim on the form the carrier
supplies.

2.2 Inspect for concealed loss or damage on the unit itself, both interior and exterior. If any, the
carrier will arrange for official inspection to substantiate your claim. Save the shipping crate until
you are sure the unit has been delivered in good condition.

2.3 If for any reason you must return the unit, contact your sales representative for authorization and
supply nameplate data.

3. INSTALLATION

3.1 Local city, county, or other ordinances may govern the use of this equipment. If you have any

questions about local requirements, please contact the appropriate local agency.

3.2 Under normal circumstances these units are intended for use indoors, at room temperatures
between 5 and 40C, at no greater than 80% relative Humidity (at 25C) and with a supply
voltage that does not vary by more than 10%. These chambers should not be operated at an
altitude exceeding 2000 meters. Installation category is CAT-II.

3.3 Pollution Degree 2. Customer service should be contacted for operating conditions outside of
these limits. Installation may be performed by the end user. It is unnecessary for this unit to be
installed by a technician.

3.4 Location: In selecting a location, consider all conditions that might affect performance, such as
heat from radiators, ovens, autoclaves, etc. Avoid direct sun, UV light sources, fast-moving air
currents, heating and cooling ducts, and high traffic areas. Allow a minimum of 10 cm between
the unit and any walls or partitions that might obstruct free airflow and to promote easy access to
the power cord and source. Under normal circumstances this unit is intended for use indoors.

3.5 Power Source: The unit power requirements are listed on the data plate. Check the data plate
for voltage, cycle, and ampere requirements. Plug the power cord into a PROPERLY
GROUNDED (earthed) Power source OF THE CORRECT SIZE AND STYLE INDICATED ON
THE UNIT NAMEPLATE RATING. A separate circuit is recommended for this unit to prevent
loss of product due to overloading or circuit failures caused by other equipment. The supply
voltage must match the nameplate voltage within +/- 10%. The US market units are designed for
120 VAC @ 60 Hz, 12.0 Amps. The EU market units are designed for 230 VAC @ 50/60Hz, 6.0
Amps. If supplied with a detachable cord set, plug the female end into the inlet on the unit and
the male plug into the supply. Assure that units requiring fuses have them installed. The fuse is a
T type, 12.5 Amp for 110 volt units, 10 Amp for 220 volt units. This fuse may be at the inlet or a
part of the cord set male plug.

3.6 Leveling: The unit must sit level and solidly. Leveling feet are supplied and must be installed in
the four holes in the bottom corners of the unit. With the feet installed and the unit standing
upright, each foot can be raised by turning it in a counterclockwise direction. Adjust the foot at
each corner until the unit stands level and solid without rocking. If the unit must be moved, turn
the leveling feet in (clockwise) all the way to prevent damage while moving.

3.7 Lifting and Handling: CAUTION: Use appropriate lifting devices that are sufficiently rated for
these loads. Failure to do so could result in minor or moderate injury. Units should only be lifted
from their bottom surfaces. Handles and knobs are not adequate for lifting or stabilization. The
unit should be completely restrained from tipping during lifting or transport. All moving parts such
as trays or covers should be removed during transfer to prevent shifting and damage.

3.8 Cleaning: Appropriate lab practice includes cleaning and disinfecting the unit prior to use. Your
operating conditions and appropriate protocol will determine the correct procedure for cleaning.
A typical cleaning procedure is described below and will help reduce the likelihood of
contamination and the necessity of decontamination. Clean the unit regularly. Depending on
usage and protocol, this may be weekly, monthly or quarterly. Cleaning the unit does not qualify
the unit as decontaminated.

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WARNING: Follow these safety precautions regardless of the cleaning procedure. Failure to
do so may cause serious injury or death.

 Always disconnect the unit from the electrical service when cleaning.
 Assure all volatile or flammable cleaners are evaporated and dry before reconnecting

the unit to the power supply.

NOTICE: Follow these precautions regardless of cleaning procedure. Failure to do so
may cause property damage and void your warranty.

 Special care should be taken when cleaning around sensing heads to prevent damage.
 The Bactrox unit was cleaned at the factory, however, a general cleaning is

recommended. Use BENZALKONIUM CHLORIDE to clean your chamber. See list of
chamber accessories products. Do not use chlorine-based bleaches, ammonium-based
disinfectants or aerosol canisters or abrasive cleaners. These will modify the powder
coated and stainless steel interior finishes and create a biologically toxic atmosphere
inside the chamber.

 Do not use hard tools such as metal wire brushes or steel wool. Use non-abrasive

cleaners and soft tools such as plastic brushes.

Typical Bactrox Cleaning Procedure

 Remove the condensate collection vessel pan every week, wash with soap and water,

and then disinfect with 70% alcohol solution. Replace in the chamber.

 Remove all shelves, shelf supports, shelf standards and shields. Wash and disinfect as

described in item 1. For initial set up, clean and disinfect shelving parts prior to
installation.

 Wash and disinfect all interior surfaces including inside the incubator.
 Replace all N2 and CO2 filters every six months or when noticeably dirty on the

upstream side. CO2 and N2 filters are located in the shadow box just behind the GAS
IN fitting and in line with the CO2 tubing kit and inside the pump box in the shadow box.

3.9 Gas Source: Install the gas regulator(s) on the tank(s) of gas. Chain the gas tanks(s) to a
secure position on the wall. Set the regulator(s) to 15 psi.

3.10 To achieve a hypoxic (1-20% O2) environment we recommend using a tank of pure Nitrogen
(N).

3.11 To achieve a hypoxic atmosphere enriched with carbon dioxide (1-20% CO2) we recommend a
tank of pure Carbon Dioxide (CO2).

3.12 N2 must be connected to N2 PORT. CO2 must be connected to the CO2 Port.

Figure 7

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4. CONTROL PANEL OVERVIEW

4.1 POWER: The I/O (ON/OFF) switch controls all of the power for the chamber and must be in the

I/ON position before any systems are operational. Both “SET TEMPERATURE” “SETO2 %” and
“SET CO2 %” displays will illuminate when the power switch is in the ON position.

4.2 SET TEMPERATURE: This controller is marked “SET TEMPERATURE” and indicates the
actual temperature within the chamber to 0.1°C. The UP/DOWN buttons are used for inputting
the set point, calibrating the display, and muting or unmuting the audible alarm. The HIGH and
LOW alarm indicators will light whenever there is an alarm condition associated with the
temperature within the chamber. The MUTE indicator will light whenever the audible alarm has
been deactivated.

4.3 HEATING ACTIVATED LIGHT Heating activated light will be lit whenever the main incubator
heating element is on.

4.4 SET OVER TEMPERATURE: This is a hydraulic thermostat that is wired between the Main
temperature controller and the heating element and functions as an override control. During
normal operation, if at any time the “SET Temperature” control fails in the ON position, and the
temperature in the incubator rises above its set point, the “SET OVER TEMPERATURE” is
activated and maintains temperature at its own set point. Note that the HEATING ACTIVATED
LIGHT indicator will not continue to function while overridden by Over Temperature Limit.

4.5 OVER TEMPERATURE ACTIVATED LIGHT: This pilot lamp is on whenever the “SET OVER
TEMPERATURE” safety thermostat has been activated and taken control of the heating

element. During normal operating conditions this indicator should never be on.

4.6 OXYGEN CONTROL: This controller is marked “SET OXYGEN %” and indicates the
percentage of O2 content within the chamber to 0.1%. The UP/DOWN buttons are used to input
the set point, calibrating the display, and muting or unmuting the audible alarm. The HIGH and
LOW alarm indicators will light whenever there is an alarm condition associated with the O2%
within the chamber. The MUTE indicator will light whenever the audible alarm has been
deactivated.

4.7 N2 INJECTING: This pilot lamp is on whenever the oxygen control has been activated and is
actively injecting N2 gas via the solenoid valve.

4.8 AIR INJECTING: This pilot lamp is on whenever the oxygen control has been activated and is
actively injecting air via the solenoid valve associated with the air supply pump.

4.9 CO2 CONTROL: This controller is marked “SET CO2 %” and indicates the percentage of CO2
content within the chamber to 0.1%. The UP/DOWN buttons are used to input the set point,
calibrating the display, and muting or unmuting the audible alarm. The HIGH and LOW alarm
indicators will light whenever there is an alarm condition associated with the CO2% within the
chamber. The MUTE indicator will light whenever the audible alarm has been deactivated.

4.10 CO2 INJECTING: This pilot lamp is on whenever the Carbon Dioxide control has been
activated and is actively injecting CO2 gas via the solenoid valve.

4.11 PASS BOX CONTROL: The “START PASS BOX PURGE” button when engaged will actively
initiate a PASS BOX purging cycle. The cycle time is ~45 seconds.

4.12 PASS BOX PURGING: This pilot light is on whenever the PASS BOX PURGE button has been
pressed. The light will blink for the duration of the ~45 second cycle.

4.13 RS232: Electrical connection for data logging and or calibration of the Oxygen, Carbon Dioxide
and Temperature controls.

5. HOSE AND ELECTRICAL CONNECTIONS

5.1 A supply hose connects from the N2 PORT fitting to a N2 tank and regulator (CGA 580).

5.2 A supply hose connects from the CO2 PORT fitting to a CO2 tank and regulator (CGA 320).

5.3 N2 and CO2 Regulator Considerations:

5.3.1 Ensure that you are using a dual stage regulator CGA 580 for N2 and CGA 320 for

CO2. It is highly recommended that a good quality 0-60 PSI output range DUAL
STAGE pressure regulator be used on the N2 tank and CO2 tanks. The dual stage
regulator will have two pressure gauges. The high pressure gauge (0-4000 PSI) will
indicate the pressure within the tank. The low pressure gauge (0-60 PSI) will indicate
the output pressure on the supply hose to the chamber. Single stage regulators do
not provide as stable performance as dual stage.

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5.3.2 Reading & Understanding Your CO2 Tank Levels: Pure CO2 is in a liquid state in the
tank, and a constant vapor pressure is generated in the tank above the liquid level.
The CO2 is drawn off of the top as a gas. It is normal for the high pressure gauge on
your regulator to start out reading 800 to 1000 PSI with a full tank. The same vapor
pressure is maintained as long as any liquid is left in the tank. The reading will drop
from 800 to 500 PSI quickly and will stay there for most of the duration of the tank. At
the end of use, the pressure will drop quickly to zero to indicate that the tank is
completely empty. When the last of the liquid has evaporated into gas then the
pressure will drop rapidly as the gas is drawn off.

5.3.3 NOTE: Pressure from the regulator to the incubator should be set at 15 PSI. Models
with additional internal regulators have pressure reduction to 10 PSI to prevent
overshoot.

5.3.4 NOTICE: Only medical grade CO2 should be used in your Bactrox. Failure to do so
may damage the unit and void your warranty.

NOTE: Pressure from the regulator to the incubator should be set at 15 PSI. An additional

internal regulator further reduces the pressure to 10 PSI to prevent overshoot.

5.4 Hose ports for the gas are provided on the upper left side of the unit. The Bactrox chamber has
five access ports on the left side of the unit. See Diagrams Section page 33 (INTERIOR
PLUMBING) for more details.

5.5 Install one end of the hose from the N2 gas regulator of the gas tank to the port marked “N2
PORT” on the plumbing connections panel.

5.6 Install one end of the hose from the CO2 gas regulator of the gas tank to the port marked “CO2
PORT” on then plumbing connections panel.

NOTE: CONNECTING HOSES. Hose connections are intended to be simple. Insert the hose into
the barb until the hose stops.

5.7 Plug power cord from Bactrox into a suitable outlet. Refer to the Installation Section of this

manual for additional information. Turn the power switch ON to verify power to the system.

5.8 When the power switch to the Hypoxic Chamber is activated, you should see a light in the back­lit power switch, the O2 and CO2 digital display panels illuminate, along with a readout of the
incubator temperature. If any of these appear not to work, consult the Troubleshooting Guide.

6. THERMOELECTRIC CONDENSATE CONTROLER

6.1 The thermoelectric condensate controller collects excess humidity and eliminates chamber

condensation. Moisture is funneled into a tube for collection inside your chamber.

6.2 The thermoelectric condensate controller is located on the left side of the chamber interior
behind circulation fan. A plastic tube drains excess moisture into the working chamber, on the
back left side.

6.3 Place a container, e.g., glass flask or beaker, under the tube for collection. Empty the container
DAILY.

6.4 Desiccants are not recommended in the chamber. Desiccants are drying agents that will pull
moisture from your samples by removing humidity and leaving condensate.

7. INCUBATOR TEMPERATURE CONTROLLER/SET OVER
TEMPERATURE

Under certain circumstances, samples placed directly on the bottom shelf may become too hot and dry out. Shelf
spacers are provided to minimize this condition. Three spacers are provided for the BACTROX. Place these
spacers on the bottom shelf. Note the slots go to the front and back.

7.1 Setting Incubator Controller: The incubator temperature controller regulates the incubator
temperature by use of Up/Down buttons. To enter set point mode on the control, press either the
Up or Down button one time. The digital display will start to blink, going from bright to dim. While
blinking, the Digital Display is showing the set point. To change the set point, use the Up and
Down buttons. If the buttons are not pressed for five (5) seconds, the display will stop blinking
and will read the temperature of the unit. Note that the SET OVER TERMPERATURE knob
should be turned to its maximum position, (clockwise) until the unit has stabilized at desired set

Bactrox Operations Manual 9

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point temperature. Allow the incubator at least 24 hours to stabilize. Then re-calibrate the digital
display to your reference thermometer, follow the calibration instructions. This incubator was
calibrated at the factory at 370C.

7.2 Setting Incubator Safety: First, set the control to the desired incubator temperature and then the
INCUBATOR SAFETY to its maximum position. Allow 24 hours for stabilization before
proceeding.

7.3 If, after 24 hours the temperature is not at desired level, adjust the SET TEMPERATURE up or
down using the arrows until the precise desired temperature is achieved.

7.4 When stabilization at the desired temperature has been achieved, turn the SET OVER
TERMPERATURE control knob counter-clockwise until the OVER TEMP ACTIVATED light
comes on and audible alarm is activated. Next, carefully turn the SET OVER TERMPERATURE
knob clockwise until the light is just off. The safety is now set; the OVER TEMP ACTIVATED
light should remain OFF during normal operation.

7.5 NOTE: An accurate thermometer should be used inside the incubator as a reference when
setting and calibrating the incubator temperature.

7.6 It is a good idea to mark the SET OVER TERMPERATURE label with the desired setting or
position as a backup in case the knob is moved accidentally. If the OVER TEMP ACTIVATED
light is on and audio alarm is activated at any time, check the SET TEMPERATURE controller
setting to be sure that it is not set above the SET OVER TERMPERATURE setting.

7.7 Alarm Controls and Display: The temperature control, O2, and the CO2 control are both
equipped with visual and audible alarms.

• Temperature Alarms: The temperature control has alarm indicators for high and low conditions

that are activated whenever the actual temperature is 1°C above or below the set point.

• O

Alarms: The O2 controller has alarm indicators for high and low alarm conditions that are

2

activated whenever the actual O2% is 1% above or below the set point.

• CO2 Alarms: The CO2 controller has alarm indicators for high and low alarm conditions that are

activated whenever the actual CO2% is 1% above or below the set point.

• Audible Alarms: Both controls have audible alarms that are activated when either of the HIGH

or LOW indicators is activated.

7.8 Muting Audible Alarms: The audible alarms can be muted for a single alarm occurrence by
pressing and holding down EITHER the UP or DOWN button for several seconds until the alarm
mutes. There is a built in delay of approximately 15 minutes on the occurrence of a LOW alarm.
This time delay prevents the audible alarm from activating every time the door is opened and the
temperature and CO2 drops.

8. CO2 CONTROL CALIBRATION

8.1 Manufacturer’s Recommendation for Monitoring CO2 Levels: CO2 sensors are factory calibrated

and, under normal circumstances, need no calibration. It is recommended that the accuracy of
your CO2 control system be monitored by measuring the actual CO2 concentration on a weekly
basis with a Fyrite or other measuring device. This should be done when the chamber has not
been opened for an extended period of time, i.e. after the weekend, and should only take 1-2
hours.

8.2 Setting the CO2 Control: Attach the supply hose from the CO2 tank to the Bactrox CO2 inlet fitting
and turn on the CO2 supply. Set the CO2 control to the desired set point using the up and down
arrows. The CO2 level comes from the factory preset to OFF.

8.3 Adjusting CO2 Display: After the Bactrox has had several hours to stabilize at the desired CO
set point and measure the actual CO2% with a Fyrite gas analyzer. If there is any difference
between the Fyrite gas analyzer and the display, use the procedure described in section 7 page
14 for calibrating CO2. See Section 9 Page 19 (next page) for Fyrite use and instruction.

NOTE: When using the Fyrite gas analyzer, insure that gas is not being injected while the reading is
being taken. Always change the CO2 set point to 0.0 prior to taking the sample and change the set point
back to the desired value after the use of the Fyrite gas analyzer is finished.

8.4 Using a FYRITE to Measure CO2 Levels

A Bacharach FYRITE CO2 Gas Analyzer is recommended to measure CO
concentrations in the Bactrox chamber. This test instrument is not supplied with the

2

2

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