Shellab BACTRONEZ User Manual
Installation and
Operation Manual
BACTRONEZ
BACTRON® 110 – 120 Volts
BACTRONEZ Anaerobic Workstation 110 - 120 Volts
Installation and Operation Manual
Part number (Manual): 4861701
Revision: August 27, 2014
The BACTRONEZ does not come with all of the accessories required for operation. Please see the
Required and Useful Accessories section on page 8 for required items and ordering information.
These units are TÜV CUE listed as Climactic Chambers (Anaerobic Chambers) for professional,
industrial, or educational use where the preparation or testing of materials is done at approximately
atmospheric pressure and no flammable, volatile, or combustible materials are being heated.
These units have been tested to the following requirements:
CAN/CSA C22.2 No. 61010-1:2012
CAN/CSA C22.2 No. 61010-2-010 + R:2009
UL 61010A-2-010:2002
UL 61010-1:2012
EN 61010-1:2010
EN 61010-2-010:2003
2 | P a g e
TABLE OF CONTENTS
INTRODUCTION ........................................................................................................................................... 5
General Safety Considerations ................................................................................................................. 5
Engineering Improvements ....................................................................................................................... 6
Contacting Assistance ............................................................................................................................... 6
RECEIVING YOUR BACTRONEZ ............................................................................................................... 7
Inspecting the Shipment ............................................................................................................................ 7
Required and Useful Accessories ............................................................................................................. 8
Returning the Shipment ............................................................................................................................ 8
Recording Data Plate Information ............................................................................................................. 9
GRAPHIC SYMBOLS ................................................................................................................................. 10
INSTALLATION .......................................................................................................................................... 12
Ambient Conditions ................................................................................................................................. 12
Location ................................................................................................................................................... 12
UV Lighting .............................................................................................................................................. 12
High Altitude Locations ........................................................................................................................... 13
Power Source .......................................................................................................................................... 13
Lifting and Handling ................................................................................................................................ 14
Leveling ................................................................................................................................................... 14
Gas Source ............................................................................................................................................. 15
BACTRONEZ Orientation ....................................................................................................................... 15
Workspace Chamber Orientation ............................................................................................................ 16
Pass Box Orientation .............................................................................................................................. 16
Cleaning .................................................................................................................................................. 17
CONTROL PANEL OVERVIEW ................................................................................................................. 18
OPERATION ............................................................................................................................................... 22
Theory of Operation and Major Component Functions ........................................................................... 22
Setting up the BACTRONEZ ................................................................................................................... 25
Set up the Gas Supply ............................................................................................................................ 27
Single Gas Connection ........................................................................................................................... 28
Dual Gas Connection .............................................................................................................................. 28
Connecting To an In-House Vacuum System ......................................................................................... 29
Connecting the Vacuum Pump ............................................................................................................... 29
Filling the Manometer .............................................................................................................................. 29
Installing the Arm Port Doors .................................................................................................................. 29
Connecting the Foot Pedal Assembly ..................................................................................................... 30
Shelving Installation ................................................................................................................................ 31
Power the BACTRONEZ ......................................................................................................................... 31
Establishing an Anaerobic environment .................................................................................................. 32
Installing the Sleeve Assemblies ............................................................................................................. 34
Chamber Entry ........................................................................................................................................ 35
Moving In The Pressurized Chamber...................................................................................................... 35
Verifying an Anaerobic Atmosphere........................................................................................................ 36
Troubleshooting The Presence of Oxygen ............................................................................................. 37
Exiting the Chamber ................................................................................................................................ 38
Setting the Incubator Temperature ......................................................................................................... 39
Setting the Over Temperature Limit ........................................................................................................ 39
Operating the Pass Box .......................................................................................................................... 40
Loading the Workstation ......................................................................................................................... 42
Reactivating the Catalyst Cartridge......................................................................................................... 42
3 | P a g e
Calibrate the Incubator Temperature display .......................................................................................... 43
Gas Conservation Methods ..................................................................................................................... 44
Activated Charcoal Scrubber and Rejuvenation Cycle ........................................................................... 45
Attaching Equipment to the Chamber Accessory Outlets ....................................................................... 45
Deionized and Distilled Water ................................................................................................................. 46
Humidifying the Incubator ....................................................................................................................... 46
Pressure Unit Conversion ....................................................................................................................... 46
USER MAINTENANCE ............................................................................................................................... 47
Daily Maintenance ................................................................................................................................... 48
Normal Gas Consumption ....................................................................................................................... 48
Leak Diagnosis – Unit Full ...................................................................................................................... 49
Leak Check – Empty Unit ........................................................................................................................ 50
Door Gasket Maintenance and Usage .................................................................................................... 51
Sleeves Maintenance and Usage ........................................................................................................... 51
Replacing the Sleeve Cuffs ..................................................................................................................... 52
Cleaning .................................................................................................................................................. 53
Disinfecting .............................................................................................................................................. 54
Vacuum Pump Maintenance ................................................................................................................... 54
Maintaining the Acrylic Glass Panels ...................................................................................................... 55
Electrical Components ............................................................................................................................ 55
Condensation and the Dew Point ............................................................................................................ 56
UNIT SPECIFICATIONS ............................................................................................................................. 57
Weight ..................................................................................................................................................... 57
Work Station Dimensions ........................................................................................................................ 57
Stand Dimensions ................................................................................................................................... 58
Incubator Dimensions ............................................................................................................................. 58
Pass Box Interior Dimensions ................................................................................................................. 58
Pass Box Plate Capacity ......................................................................................................................... 58
Pass Box Volume .................................................................................................................................... 58
Chamber Capacity................................................................................................................................... 59
Total Plate Capacity ................................................................................................................................ 59
Temperature ............................................................................................................................................ 59
Power ...................................................................................................................................................... 59
PARTS LIST ............................................................................................................................................... 60
Ordering Parts and Consumables ........................................................................................................... 61
ACCESORIES ............................................................................................................................................ 62
4 | P a g e
INTRODUCTION
Thank you for purchasing a Shel Lab BACTRONEZ Anaerobic Chamber Workstation. We know
that in today’s competitive marketplace, customers have many choices when it comes to constant
temperature equipment. We appreciate you choosing ours. Our continued reputation as a leading
laboratory product manufacturer rests with your satisfaction. Sheldon Manufacturing, Inc. stands
behind our products, and we will be there if you need us.
BACTRONEZ workstations are intended for professional, industrial, and educational applications as
anaerobic workstations suitable for the cultivation of clinical bacteria. These workstations are not
intended for use at hazardous or household locations. Only use this equipment for its intended
spectrum of applications; any alterations or modifications void the warranty.
Before using the BACTRONEZ read this entire manual carefully to understand how to install,
operate, and maintain the workstation in a safe manner. Keep this manual available for use by all
workstation operators. Ensure that all operators are given appropriate training prior to using the
BACTRON.
GENERAL SAFETY CONSIDERATIONS
Note: Failure to follow the guidelines and instructions in this manual may create a protection
impairment by disabling or interfering with the unit’s safety features. This can result in injury
or death.
Your BACTRONEZ and its recommended accessories are designed and tested to meet strict safety
requirements. The workstation is built to connect to a wall power source using the specific power
cord type shipped with the unit.
For continued safe operation of your BACTRONEZ, always follow basic safety precautions
including:
Follow all local or regional ordinances in your area regarding the use of this unit. If you
have any questions about local regulations, please contact the appropriate agency.
Use only approved accessories. Do not modify system components. Any alterations or
modifications to your BACTRONEZ can be dangerous and void your warranty.
Always plug the BACTRONEZ power cord into an earth grounded electrical outlet that
conforms to national and local electrical codes. If the workstation is not grounded properly,
parts such as knobs and controls can conduct electricity and cause serious injury.
Avoid damaging the power cord. Do not bend it excessively, step on it, or place heavy
objects on it. A damaged cord can be a shock or fire hazard. Never use a power cord if it is
damaged.
Do not position the workstation in such a manner as to make it difficult to unplug the unit in
the event of an emergency.
Ensure that the vacuum line from the vacuum pump to the workstation is not kinked, and
that the pump is placed in a location with adequate ventilation to avoid overheating.
Do not attempt to move the workstation while in operation.
5 | P a g e
INTRODUCTION (CONTINUED)
ENGINEERING IMPROVEMENTS
Sheldon Manufacturing continually improves all of its products. As a result, engineering changes
and improvements are made from time to time. Therefore, some changes, modifications, and
improvements may not be covered in this manual. If your unit’s operating characteristics or
appearance differs from those described in this manual, please contact your Shel Lab dealer or
distributor for assistance.
CONTACTING ASSISTANCE
If you are unable to resolve a technical issue with the BACTRON, please contact Sheldon
Technical Support. Phone hours for Sheldon Technical Support are 6am – 4:30pm Pacific Coast
Time (west coast of the United States, UTC -8).
Please have the following information ready when calling or emailing Technical Support: the model
number and the serial number. These will be found on the unit’s data plate, which is located in the
workspace chamber next to the inner pass box door. See page 9.
EMAIL: tech@shellab.com PHONE: 1-800-322-4897 extension 2 or (503) 640-3000 FAX: (503)
640-1366
Sheldon Manufacturing INC.
P.O. Box 627
Cornelius, OR 97113
6 | P a g e
Anaerobic Indicator
Strips (5 packets)
2 Arm Port Doors,
Left and Right
PN 9900699 / PN 9900698
Shelf Spacers (3)
Sleeve Cuff
Assemblies
2 (Size 8 Medium)
Catalyst Cartridge
(1)
Power Cord 5-15
NEMA
(1)
RECEIVING YOUR BACTRONEZ
INSPECTING THE SHIPMENT
Before leaving our factory, all BACTRONEZs are packaged in high-quality shipping materials to
provide protection from transportation-related damage. When the unit departs the factory, safe
delivery becomes the responsibility of the carrier. Damage sustained during transit is not covered
by the BACTRONEZ’s warranty.
This makes it important that you inspect your BACTRONEZ for concealed loss or damage to its
interior and exterior when receiving it. If you find any damage to the workstation, follow the carrier’s
procedure for claiming damage or loss. If the carton is not damaged, open the carton and remove
the contents. The unit should come with an Installation and Operation Manual, warranty card, and a
Certificate of Compliance.
Verify that the correct number of components are included with the unit:
Carefully check all packaging before discarding. Save the shipping carton until you are certain that
the unit and its accessories function properly.
7 | P a g e
Catalyst Cartridge
Foot Pedal Unit
Gas Regulator, AMG
LED Lamp Unit
Petrie Dish Rack
(2 X 11 inches)
Vacuum Pump 120V
RECEIVING (CONTINUED)
REQUIRED AND USEFUL ACCESSORIES
The BACTRONEZ Does not include a vacuum pump, foot pedal assembly, an additional catalyst
for use when the factory installed catalyst needs to be regenerated, or an AMG gas regulator. It
also does not come with an LED viewing light or Petri plate racks.
A vacuum source, foot pedal assembly, AMG gas regulator, and second catalyst are necessary for
the operation of the BACTRONEZ.
Order part number 917-995-0005 to purchase all these items as a package. Items may also be
purchased individually. Please see the Shel Lab website for pricing.
RETURNING THE SHIPMENT
If you must return the BACTRONEZ for any reason, contact your Shel Lab service representative
for a return of material authorization (RMA). You must provide the unit’s data plate information for
an RMA. See Recording Data Plate Information on the next page.
Returns without RMAs will not be accepted. BACTRONEZs that have been exposed to
contaminants such as, pathogenic microorganisms or toxic substances, are required to undergo a
decontamination procedure prior to the issuance of an RMA and shipping. Contact your service
representative for details.
8 | P a g e
Model Number
Serial Number
RECEIVING (CONTINUED)
RECORDING DATA PLATE INFORMATION
Locate the data plate in the workspace chamber above the inner pass box door. The data plate
contains the BACTRONEZ’s model number and serial number. Enter this information below for
future reference.
Date Plate Information
9 | P a g e
Symbol
Definition
Indicates that you should consult your service manual for further instructions.
Indique que l'opérateur doit consulter le manuel d'utilisation pour y trouver les instructions
complémentaires.
Indicates Temperature
Repère température
Indicates the Over Temperature Limit system
Indique le système de dépassement de temperature
Indicates AC Power
Repère le courant alternatif
Indicates I/ON and O/OFF
I repère de la position MARCHE de l'interrupteur d'alimentation
O repère de la position ARRÊT de l'interrupteur d'alimentation
Indicates protective earth ground
Repère terre électrique
Indicates UP and DOWN respectively
Touches de déplacements respectifs vers le HAUT et le BA
Indicates Manually Adjustable
Indique un bouton réglable manuellement
Indicates Potential Shock Hazard
Signale danger électrique
GRAPHIC SYMBOLS
The BACTRONEZ is provided with multiple graphic symbols located on its exterior and interior
surfaces. The symbols identify hazards and the functions of the adjustable components, as well as
important notes found in the user manual.
10 | P a g e
Symbol
Definition
WEEE Directive compliant logo
Indicates the unit should be recycled (Not disposed of in land-fill)
Indique l’appareil doit être recyclé (Ne pas jeter dans une décharge)
Indicates an anaerobic environment in the pass box
Indique un environnement anaérobie dans le sas
Indicates an aerobic environment in the pass box
Indique un environnement aérobie dans le sas
Indicates a injection of gas
Indique un flux de gazeux
Indicates the vacuum pump is evacuating the pass box
Indique la pompe à vide se vide dans le sas
Indicates the incubator heater is active
L'élément chauffant est la production de chaleur
Indicates the pass box doors are closed
Indique les portes du sas sont fermés
GRAPHIC SYMBOLS (CONTINUED)
11 | P a g e
INSTALLATION
AMBIENT CONDITIONS
This workstation is intended for use indoors, at room temperatures between 15C and 30C (59F
and 86F), at no greater than 80% Relative Humidity (at 25C / 77F). Allow a minimum of 4
inches (10cm) between the workstation and walls or partitions, and 2 inches (5cm) of clearance
above the top of the workstation for unobstructed airflow.
Operating the unit outside of these conditions may adversely affect the unit’s temperature
range and stability.
For conditions outside of those listed above, please contact your distributor or Sheldon Sales to
explore other unit options suited to your laboratory or production environment.
LOCATION
When selecting a location to install your BACTRONEZ, consider environmental conditions that can
affect the workstation’s temperature and atmospheric integrity:
Ovens, autoclaves, and any device that produces significant radiant heat
Heating and cooling ducts, or other sources of fast moving air currents
High-traffic areas
Direct sunlight
Note: Direct exposure to air conditioning vents or other sources of cold air can result in
condensation or fogging on the workstation’s acrylic glass panels, depending on humidity
and other ambient conditions. Prolonged exposure to cold air flows may adversely affect the
temperature performance of the incubator.
UV LIGHTING
Check if your laboratory or workspace contains sources of UV lighting. Sustained exposure to direct
sunlight, UVC or UV germicidal lighting around 254nm, will cause a rapid aging of BACTRONEZ
acrylic glass panels and arm port sleeves. Periodic use of long-wave (365nm) UV hand lamps for
bacterial identification should not damage the acrylic glass. See the Maintaining the Acrylic Glass
Panels entry on page 55 for more details.
12 | P a g e
INSTALLATION (CONTINUED)
HIGH ALTITUDE LOCATIONS
BACTRONEZ anaerobic workstations are equipped with two (2) digital vacuum controller - display
units that control the atmospheric cycling operations of the pass box and the vacuum pump line
pressure. These units are calibrated at the factory at an altitude of 179 feet (54.5m) above sea
level, and read gauge pressure rather than absolute pressure. Altitudes around 6500 feet (2000m)
or higher may affect the operation of the vacuum system, depending on ambient conditions. If the
vacuum pump runs continuously or near continuously when installed at a high altitude location,
contact Sheldon Technical Support (see page 6) for assistance in adjusting the vacuum gauge
settings.
POWER SOURCE
Always position the workstation so that the users have access to the power cord and can quickly
unplug it in the event of an emergency.
When choosing a location for the BACTRONEZ check that the voltage and ampere requirements
on the workstation’s data plate match those of your wall source. The source must be an earth
grounded outletThe supplied voltage must not vary more than 10% from the data plate rating.
Damage to the workstation may result if supplied voltage varies more than 10%. Each
BACTRONEZ workstation is provided with a 110 – 120VAC 8ft (2.5m) 5-15 NEMA power cord.
These incubators are intended for a 50/60 Hz, 110 - 120 volt applications at the 9 Amps
Use a separate circuit to prevent loss of the unit due to overloading or circuit failure.
Note: Electrical supply to the BACTRONEZ must conform to all national and local electrical codes.
13 | P a g e
INSTALLATION (CONTINUED)
LIFTING AND HANDLING
The BACTRONEZ is heavy, and care should be taken to use appropriate lifting devices that are
sufficiently rated for these loads. Follow these guidelines when lifting and handling the
BACTRONEZ workstation:
Lift the BACTRONEZ only from its bottom surface.
Doors, handles, and knobs are not adequate for lifting or stabilization.
Restrain the BACTRONEZ completely while lifting or transporting so it cannot tip.
Remove all moving parts, such as shelf spacers and trays, and secure all doors in the
closed position during transfer to prevent shifting and damage.
Note: To prevent damage when moving the BACTRONEZ, turn each of the four leveling feet
completely clockwise.
LEVELING
The BACTRONEZ must be level and stable for safe operation. Each BACTRONEZ ships with four
leveling feet. Insert one leveling foot into each of the four holes in the bottom corners of the
workstation. Adjust each foot at each corner until the workstation stands level and solid without
rocking. To raise a foot, turn it in a counterclockwise direction; to lower a foot, turn it in a clockwise
direction.
14 | P a g e
Catalyst
Cartridge
Manometer
Workspace Chamber
Incubator
Arm Ports
Pass Box
Main Control
Panel
Gas and Vacuum
Panel
INSTALLATION (CONTINUED)
GAS SOURCE
Warning: Never exceed a 5% hydrogen concentration inside the unit’s chamber.
Avertissement: La concentration d’hydrogène ne doit pas dépasser 5% dans la chambre anaérobie.
The BACTRONEZ requires an anaerobic gas mixture with 5% hydrogen to drive the oxygencapturing catalytic process in the workspace chamber. To reduce anaerobic gas consumption
during pass box auto cycles, the BACTRONEZ may also be connected to a second compressed
gas cylinder containing an inert gas such as, nitrogen (N2), for use in the first two stages of the
three-stage auto cycle. Manual cycles of the pass box will only draw from the anaerobic
mixed gas cylinder attached to the GAS 1 In port.
For a single cylinder of anaerobic mixed gas (AMG), Sheldon Manufacturing recommends
5% hydrogen (H2), 5% Carbon Dioxide (CO2), and 90% Nitrogen (N2).
If you are using a two gas anaerobic application, Sheldon recommends one cylinder of
AMG gas at the above ratios, and a second cylinder of 100% Nitrogen (N2).
Gas regulators for BACTRONEZ applications will need to be set at 15 - 20 psi. Please see
the Pressure Unit Conversion table on page 46 in the Operation Section for working with
units other than Pounds per Square Inch.
Anaerobic Mixed Gas is often sold by gas suppliers under the category of Anaerobic Incubation
Mixtures or Biological Atmospheres. Contact your site safety officer and review your institutional
safety protocols for handling, storing, and using compressed gasses. Follow all local ordinances
and national regulations regarding compressed gases in research, clinical, or production
environments.
BACTRONEZ ORIENTATION
15 | P a g e
Catalyst
Cartridge
and
Handel
Data Plate
INSTALLATION (CONTINUED)
WORKSPACE CHAMBER ORIENTATION
Figure 1: Workspace Chamber Layout
PASS BOX ORIENTATION
Figure 2: Pass Box Inner Door and Sliding Shelf
The pass box is provided with an integral sliding shelf to facilitate material transfers. Small items
may be introduced into the chamber via the Arm Port Doors and Sleeve Assemblies using proper
sleeve technique.
16 | P a g e
INSTALLATION (CONTINUED)
CLEANING
The BACTRONEZ interior was cleaned and disinfected at the factory, but not sterilized. See the
Cleaning procedure in the User Maintenance section for more information.
17 | P a g e
CONTROL PANEL OVERVIEW
Figure3: Main Control Panel
Power Switch
The main power switch on the control panel controls all power to the workstation and must be in the
on ( I ) position before any of the BACTRONEZ’s systems are powered. The switch will illuminate
when on.
Chamber Gas Light
This light indicates that anaerobic mixed gas is being injected into the workspace chamber.
Main Temperature Control and Green Digital Display
This control is marked SET TEMPERATURE and consists of up and down arrow buttons for
inputting a set point temperature and switching to calibration mode. The green digital display shows
the incubator’s temperature in its default display, accurate to within + 0.1C, as well as the
temperature set point when the arrow keys are being used to make set point adjustments. In
calibration mode, the control and display are used to enter temperature offsets to match the
BACTRONEZ’s displayed temperature to the temperature detected by an independent calibration
(reference) thermometer.
18 | P a g e
CONTROL PANEL OVERVIEW (CONTINUED)
Heating Activated Light
The clear pilot light located beneath the label HEATING ACTIVATED illuminates whenever the
heating elements are powered and warming the incubator.
Set Over Temperature
This graduated dial sets the incubator’s temperature limit for the Over Temperature Limit backup
system. The OTL System operates independently of the Main Temperature Control, and prevents
uncontrolled heating of the incubator in the event of a failure of the Main Controller while in its
heating mode. For more details, please see the explanation of the Over Temperature Limit
System in the Operation section’s Theory of Operation.
OTL Light
This pilot light is marked OVERTEMPERATURE ACTIVATED and is directly below the Over
Temperature Limit control. The light will turn on when the OTL System has taken control of the
incubator, and will be accompanied by a buzzer alarm. Under normal operating conditions this light
should not illuminate.
Pass Box Doors Light
The clear Doors pilot light indicates that both pass box doors are closed. This light must be on in
order to cycle the pass box.
Anaerobic Light
This is light activates after the completion of a full auto cycle of the pass box Interior.
Aerobic Light
This light activates when the outer pass box door has been opened, exposing the pass box to
aerobic atmosphere. It remains on until the next completion of a full auto cycle.
19 | P a g e
CONTROL PANEL OVERVIEW (CONTINUED)
Pass Box Vacuum Controller and Digital Display
The main control panel vacuum gauge-controller displays the level of vacuum in inches of mercury
in the pass box interior. It controls the application of vacuum to the pass box during the pass box
auto cycle.
Figure 4: Pass Box Vacuum
Controller and Display
Automatic Start / Reset Switch
The all black Automatic Start / Reset switch initiates or aborts the pass box’s auto vacuum-andgas-recharge cycle used to remove aerobic atmosphere from the pass box. Pressing then releasing
on the left (Start) the will initiate the auto cycle. Pressing and holding on the right (Reset) for three
seconds will abort and then restore the pass box to a near standard atmospheric pressure sufficient
that the pass box doors can be easily opened.
Pass Box Gas Light
This light indicates that the anaerobic mixed gas is being injected into the pass box during the auto
cycle. The pass box Gas light does not illuminate during manually gas injections.
20 | P a g e
Loading...