Shellab 600 User Manual
Installation and
Operation Manual
BACTRON300, BACTRON600, BACTRON900
Previously Designated:
BACTRONII, BACTRONIV, BACTRONIV-900
BACTRON® 110 – 120 Volts
BACTRON Anaerobic Workstation 110 - 120 Volts
Installation and Operation Manual
Part number (Manual): 4861700-1
Revision: August 27, 2014
Pictured on Cover: BACTRON900 (BACTRONIV-900)
BACTRON600 (BACTRONIV)
BACTRON300 (BACTRONII)
These units are TÜV CUE listed as Climactic Chambers (Anaerobic Chambers) for professional,
industrial, or educational use where the preparation or testing of materials is done at approximately
atmospheric pressure and no flammable, volatile, or combustible materials are being heated.
These units have been tested to the following requirements:
CAN/CSA C22.2 No. 61010-1:2012
CAN/CSA C22.2 No. 61010-2-010 + R:2009
UL 61010A-2-010:2002
UL 61010-1:2012
EN 61010-1:2010
EN 61010-2-010:2003
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TABLE OF CONTENTS
INTRODUCTION ........................................................................................................................................... 5
General Safety Considerations ................................................................................................................. 5
Engineering Improvements ....................................................................................................................... 6
Contacting Assistance ............................................................................................................................... 6
RECEIVING YOUR BACTRON .................................................................................................................... 7
Inspecting the Shipment ............................................................................................................................ 7
Returning the Shipment ............................................................................................................................ 8
Recording Data Plate Information ............................................................................................................. 8
GRAPHIC SYMBOLS ................................................................................................................................... 9
INSTALLATION .......................................................................................................................................... 11
Ambient Conditions ................................................................................................................................. 11
Location ................................................................................................................................................... 11
UV Lighting .............................................................................................................................................. 11
High Altitude Locations ........................................................................................................................... 12
Power Source .......................................................................................................................................... 12
Lifting and Handling ................................................................................................................................ 13
Leveling ................................................................................................................................................... 13
Gas Source ............................................................................................................................................. 14
BACTRON900 Orientation ...................................................................................................................... 15
BACTRON600 Orientation ...................................................................................................................... 15
BACTRON300 Orientation ...................................................................................................................... 16
Workspace Chamber Orientation (All Units) ........................................................................................... 17
Pass Box Orientation .............................................................................................................................. 17
Cleaning .................................................................................................................................................. 18
CONTROL PANEL OVERVIEW ................................................................................................................. 19
OPERATION ............................................................................................................................................... 24
Theory of Operation and Major Component Functions ........................................................................... 24
Setting up the BACTRON ....................................................................................................................... 27
Set up the Gas Supply ............................................................................................................................ 29
Single Gas Connection ........................................................................................................................... 30
Dual Gas Connection .............................................................................................................................. 30
Connecting To an In-House Vacuum System ......................................................................................... 31
Connecting the Vacuum Pump ............................................................................................................... 31
Filling the Manometer .............................................................................................................................. 31
Installing the Arm Port Doors .................................................................................................................. 31
Connecting the Foot Pedal Assembly ..................................................................................................... 32
Shelving Installation BACTRON300 and BACTRON900 ........................................................................ 33
Power the BACTRON ............................................................................................................................. 33
Establishing an Anaerobic environment .................................................................................................. 34
Installing the Sleeve Assemblies ............................................................................................................. 36
Chamber Entry ........................................................................................................................................ 37
Moving in the Pressurized Chamber ....................................................................................................... 37
Verifying an Anaerobic Atmosphere........................................................................................................ 38
Troubleshooting The Presence of Oxygen ............................................................................................. 39
Exiting the Chamber ................................................................................................................................ 40
Setting the Incubator Temperature ......................................................................................................... 41
Setting the Over Temperature Limit ........................................................................................................ 41
Operating the Pass Box .......................................................................................................................... 42
Loading the Workstation ......................................................................................................................... 44
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Reactivating the Catalyst Cartridge......................................................................................................... 45
Calibrate the Incubator Temperature display .......................................................................................... 46
Gas Conservation Methods ..................................................................................................................... 47
Activated Charcoal Scrubber and Rejuvenation Cycle ........................................................................... 48
Attaching Equipment to the Chamber Accessory Outlets ....................................................................... 48
Deionized and Distilled Water ................................................................................................................. 49
Humidifying the Incubator ....................................................................................................................... 49
Pressure Unit Conversion ....................................................................................................................... 49
USER MAINTENANCE ............................................................................................................................... 50
Daily Maintenance ................................................................................................................................... 51
Normal Gas Consumption ....................................................................................................................... 51
Leak Diagnosis – Unit Full ...................................................................................................................... 52
Leak Check – Empty Unit ........................................................................................................................ 53
Door Gasket Maintenance and Usage .................................................................................................... 54
Sleeves Maintenance and Usage ........................................................................................................... 54
Replacing the Sleeve Cuffs ..................................................................................................................... 55
Cleaning .................................................................................................................................................. 56
Disinfecting .............................................................................................................................................. 57
Vacuum Pump Maintenance ................................................................................................................... 57
Maintaining the Acrylic Glass Panels ...................................................................................................... 58
Electrical Components ............................................................................................................................ 58
Condensation and the Dew Point ............................................................................................................ 59
UNIT SPECIFICATIONS ............................................................................................................................. 60
Weight ..................................................................................................................................................... 60
Workstation Dimensions ......................................................................................................................... 60
Stand Dimensions ................................................................................................................................... 61
Pass Box Interior Dimensions ................................................................................................................. 61
Pass Box Plate Capacity ......................................................................................................................... 61
Incubator Dimensions ............................................................................................................................. 61
Capacity .................................................................................................................................................. 62
Total Plate Capacity ................................................................................................................................ 63
Temperature ............................................................................................................................................ 63
Power ...................................................................................................................................................... 63
PARTS LIST ............................................................................................................................................... 64
Ordering Parts and Consumables ........................................................................................................... 65
ACCESORIES ............................................................................................................................................ 66
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INTRODUCTION
Thank you for purchasing a Shel Lab BACTRON Anaerobic Chamber Workstation. We know that in
today’s competitive marketplace, customers have many choices when it comes to constant
temperature equipment. We appreciate you choosing ours. Our continued reputation as a leading
laboratory product manufacturer rests with your satisfaction. Sheldon Manufacturing, Inc. stands
behind our products, and we will be there if you need us.
BACTRON workstations are intended for professional, industrial, and educational applications as
anaerobic workstations suitable for the cultivation of clinical bacteria. These units are not intended
for use at hazardous or household locations. Only use this equipment for its intended spectrum of
applications; any alterations or modifications void the warranty.
Before using the BACTRON read this entire manual carefully to understand how to install, operate,
and maintain the workstation in a safe manner. Keep this manual available for use by all
workstation operators. Ensure that all operators are given appropriate training prior to using the
BACTRON.
GENERAL SAFETY CONSIDERATIONS
Note: Failure to follow the guidelines and instructions in this manual may create a protection
impairment by disabling or interfering with the unit’s safety features. This can result in injury
or death.
Your BACTRON and its recommended accessories are designed and tested to meet strict safety
requirements. The workstation is built to connect to a wall power source using the specific power
cord type shipped with the unit.
For continued safe operation of your BACTRON, always follow basic safety precautions including:
Follow all local or regional ordinances in your area regarding the use of this unit. If you
have any questions about local regulations, please contact the appropriate agency.
Use only approved accessories. Do not modify system components. Any alterations or
modifications to your BACTRON can be dangerous and void your warranty.
Always plug the BACTRON power cord into an earth grounded electrical outlet that
conforms to national and local electrical codes. If the workstation is not grounded properly,
parts such as knobs and controls can conduct electricity and cause serious injury.
Avoid damaging the power cord. Do not bend it excessively, step on it, or place heavy
objects on it. A damaged cord can be a shock or fire hazard. Never use a power cord if it is
damaged.
Do not position the workstation in such a manner as to make it difficult to unplug the unit in
the event of an emergency.
Ensure that the vacuum line from the vacuum pump to the workstation is not kinked, and
that the pump is placed in a location with adequate ventilation to avoid overheating.
Do not attempt to move the workstation while in operation.
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INTRODUCTION (CONTINUED)
ENGINEERING IMPROVEMENTS
Sheldon Manufacturing continually improves all of its products. As a result, engineering changes
and improvements are made from time to time. Therefore, some changes, modifications, and
improvements may not be covered in this manual. If your unit’s operating characteristics or
appearance differs from those described in this manual, please contact your Shel Lab dealer or
distributor for assistance.
CONTACTING ASSISTANCE
If you are unable to resolve a technical issue with the BACTRON, please contact Sheldon
Technical Support. Phone hours for Sheldon Technical Support are 6am – 4:30pm Pacific Coast
Time (west coast of the United States, UTC -8).
Please have the following information ready when calling or emailing Technical Support: the model
number and the serial number. These will be found on the unit’s data plate, which is located in the
workspace chamber next to the inner pass box door. See page 8.
EMAIL: tech@shellab.com PHONE: 1-800-322-4897 extension 4 or (503) 640-3000 FAX: (503)
640-1366
Sheldon Manufacturing INC.
P.O. Box 627
Cornelius, OR 97113
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Anaerobic Indicator
Strips (5 packets)
Arm Port Door
Left and Right
PN 9900699 / PN 9900698
BACTRON300 / 900
Shelf Spacers
BACTRON600
Arm Port Door Stands
Catalyst Cartridges
(2)
Foot Pedal Unit
Gas Regulator, AMG
LED Lamp Unit
Leveling Feet
(4)
BACTRON300
Petrie Dish Rack
7 (2 x 11)
BACTRON600
Petrie Dish Rack
10 (2 x 13)
BACTRON900
Petrie Dish Racks
7 (2 x 11) 10 (2 x 13)
Power Cord 5-15
NEMA
Vacuum Pump
Sleeve Cuff
Assemblies
2 (Size 8 Medium)
RECEIVING YOUR BACTRON
Before leaving our factory, all BACTRONs are packaged in high-quality shipping materials to
provide protection from transportation-related damage. When the unit departs the factory, safe
delivery becomes the responsibility of the carrier. Damage sustained during transit is not covered
by the BACTRON’s warranty.
This makes it important that you inspect your BACTRON for concealed loss or damage to its
interior and exterior when receiving it. If you find any damage to the workstation, follow the carrier’s
procedure for claiming damage or loss.
INSPECTING THE SHIPMENT
Carefully inspect the shipping carton for damage. Report any damage to the carrier service that
delivered the BACTRON. If the carton is not damaged, open the carton and remove the contents.
The unit should come with an Installation and Operation Manual, warranty card, and a Certificate of
Compliance.
Verify that the correct number of components are included with the unit:
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Model Number
Serial Number
RECEIVING YOUR BACTRON (CONTINUED)
Carefully check all packaging before discarding. Save the shipping carton until you are certain that
the unit and its accessories function properly.
RETURNING THE SHIPMENT
If you must return the BACTRON for any reason, contact your Shel Lab service representative for a
return of material authorization (RMA). You must provide the unit’s data plate information for an
RMA. See Recording Data Plate Information below. Returns without RMAs will not be accepted.
BACTRONs that have been exposed to contaminants such as, pathogenic microorganisms or toxic
substances, are required to undergo a decontamination procedure prior to the issuance of an RMA
and shipping. Contact your service representative for details.
RECORDING DATA PLATE INFORMATION
Locate the data plate in the workspace chamber above the inner pass box door. The data plate
contains the BACTRON’s model number and serial number. Enter this information below for future
reference.
Date Plate Information
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Symbol
Definition
Indicates that you should consult your service manual for further instructions.
Indique que l'opérateur doit consulter le manuel d'utilisation pour y trouver les instructions
complémentaires.
Indicates Temperature
Repère température
Indicates the Over Temperature Limit system
Indique le système de dépassement de temperature
Indicates AC Power
Repère le courant alternatif
Indicates I/ON and O/OFF
I repère de la position MARCHE de l'interrupteur d'alimentation
O repère de la position ARRÊT de l'interrupteur d'alimentation
Indicates protective earth ground
Repère terre électrique
Indicates UP and DOWN respectively
Touches de déplacements respectifs vers le HAUT et le BA
Indicates Manually Adjustable
Indique un bouton réglable manuellement
Indicates Potential Shock Hazard
Signale danger électrique
GRAPHIC SYMBOLS
The BACTRON is provided with multiple graphic symbols located on its exterior and interior
surfaces. The symbols identify hazards and the functions of the adjustable components, as well as
important notes found in the user manual.
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Symbol
Definition
WEEE Directive compliant logo
Indicates the unit should be recycled (Not disposed of in land-fill)
Indique l’appareil doit être recyclé (Ne pas jeter dans une décharge)
Indicates an anaerobic environment in the pass box
Indique un environnement anaérobie dans le sas
Indicates an aerobic environment in the pass box
Indique un environnement aérobie dans le sas
Indicates a injection of gas
Indique un flux de gazeux
Indicates the vacuum pump is evacuating the pass box
Indique la pompe à vide se vide dans le sas
Indicates the incubator heater is active
L'élément chauffant est la production de chaleur
Indicates the pass box doors are closed
Indique les portes du sas sont fermés
GRAPHIC SYMBOLS (CONTINUED)
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INSTALLATION
AMBIENT CONDITIONS
This workstation is intended for use indoors, at room temperatures between 15C and 30C (59F
and 86F), at no greater than 80% Relative Humidity (at 25C / 77F). Allow a minimum of 4
inches (10cm) between the workstation and walls or partitions, and 2 inches (5cm) of clearance
above the top of the workstation for unobstructed airflow.
Operating the unit outside of these conditions may adversely affect the unit’s temperature
range and stability.
For conditions outside of those listed above, please contact your distributor or Sheldon Sales to
explore other unit options suited to your laboratory or production environment.
LOCATION
When selecting a location to install your BACTRON, consider environmental conditions that can
affect the workstation’s temperature and atmospheric integrity:
Ovens, autoclaves, and any device that produces significant radiant heat
Heating and cooling ducts, or other sources of fast moving air currents
High-traffic areas
Direct sunlight
Note: Direct exposure to air conditioning vents or other sources of cold air can result in
condensation or fogging on the workstation’s acrylic glass panels, depending on humidity
and other ambient conditions. Prolonged exposure to cold air flows may adversely affect the
temperature performance of the incubator.
UV LIGHTING
Check if your laboratory or workspace contains sources of UV lighting. Sustained exposure to direct
sunlight, UVC or UV germicidal lighting around 254nm, will cause a rapid aging of BACTRON
acrylic glass panels and arm port sleeves. Periodic use of long-wave (365nm) UV hand lamps for
bacterial identification should not damage the acrylic glass. See the Maintaining the Acrylic Glass
Panels entry on page 58 for more details.
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Model
Amperage
BACTRON300 (BACTRONII)
9 Amps
BACTRON600 (BACTRONIV)
11 Amps
BACTRON900 (BACTRONIV-900)
14 Amps
INSTALLATION (CONTINUED)
HIGH ALTITUDE LOCATIONS
BACTRON anaerobic workstations are equipped with two (2) digital vacuum controller - display
units that control the atmospheric cycling operations of the pass box and the vacuum pump line
pressure. These devices are calibrated at the factory at an altitude of 179 feet (54.5m) above sea
level, and read gauge pressure rather than absolute pressure. Altitudes around 6500 feet (2000m)
or higher may affect the operation of the vacuum system, depending on ambient conditions. If the
vacuum pump runs continuously or near continuously when installed at a high altitude location,
contact Sheldon Technical Support (see page 6) for assistance in adjusting the vacuum gauge
settings.
POWER SOURCE
Note: The electrical supply to the BACTRON must conform to all national and local electrical codes.
Always position the workstation so that the users have access to the power cord and can quickly
unplug it in the event of an emergency.
When choosing a location for the BACTRON check that the voltage and ampere requirements on
the workstation’s data plate match those of your wall source. The source must be an earth
grounded outlet The supplied voltage must not vary more than 10% from the data plate rating.
Damage to the workstation may result if supplied voltage varies more than 10%. Each
BACTRON workstation is provided with a 110 – 120VAC 8ft (2.5m) 5-15 NEMA power cord.
These incubators are intended for a 50/60 Hz, 110 - 120 volt applications at the following
amperages:
Use a separate circuit to prevent loss of the unit due to overloading or circuit failure.
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INSTALLATION (CONTINUED)
LIFTING AND HANDLING
The BACTRON is heavy, and care should be taken to use appropriate lifting devices that are
sufficiently rated for these loads. Follow these guidelines when lifting and handling the BACTRON
workstation:
Lift the BACTRON only from its bottom surface.
Doors, handles, and knobs are not adequate for lifting or stabilization.
Restrain the BACTRON completely while lifting or transporting so it cannot tip.
Remove all moving parts, such as shelf spacers and trays, and secure all doors in the
closed position during transfer to prevent shifting and damage.
Note: To prevent damage when moving the BACTRON, turn each of the four leveling feet
completely clockwise.
LEVELING
The BACTRON must be level and stable for safe operation. Each BACTRON ships with four
leveling feet. Insert one leveling foot into each of the four holes in the bottom corners of the
workstation. Adjust the foot at each corner until the workstation stands level and solid without
rocking. To raise a foot, turn it in a counterclockwise direction; to lower a foot, turn it in a clockwise
direction.
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INSTALLATION (CONTINUED)
GAS SOURCE
Warning: Never exceed a 5% hydrogen concentration inside the unit’s chamber.
Avertissement: La concentration d’hydrogène ne doit pas dépasser 5% dans la chambre anaérobie.
The BACTRON requires an anaerobic gas mixture with 5% hydrogen to drive the oxygen-capturing
catalytic process in the workspace chamber. To reduce anaerobic gas consumption during auto
cycle pass box purges, the BACTRON may also be connected to a second compressed gas
cylinder containing an inert gas such as, nitrogen (N2), for use in the first two stages of the threestage auto cycle. Manual cycles of the pass box will only draw from the anaerobic mixed gas
cylinder attached to the GAS 1 In port.
For a single cylinder of anaerobic mixed gas (AMG), Sheldon Manufacturing recommends
5% hydrogen (H2), 5% Carbon Dioxide (CO2), and 90% Nitrogen (N2).
If you are using a two gas anaerobic application, Sheldon recommends one cylinder of
AMG gas at the above ratios, and a second cylinder of 100% Nitrogen (N2).
Gas regulators for BACTRON applications will need to be set at 15 - 20 psi. Please see the
Pressure Unit Conversion table on page 49 in the Operation Section for working with
units other than Pounds per Square Inch.
Anaerobic Mixed Gas is often sold by gas suppliers under the category of Anaerobic Incubation
Mixtures or Biological Atmospheres. Contact your site safety officer and review your institutional
safety protocols for handling, storing, and using compressed gasses. Follow all local ordinances
and national regulations regarding compressed gases in research, clinical, or production
environments.
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Gas and
Vacuum Ports
Main Control
Panel
Manometer
Side Storage
Incubator
Pass Box
Arm Ports
LED
Lamp
Catalyst
Cartridge
Workspace
Chamber
Workspace
Chamber
Incubator
Catalyst
Cartridge
Gas and
Vacuum
Ports
Workspace Incubator
Control Panel
Manometer
Side
Storage
Incubator
Pass Box
Arm Ports
LED Lamp
Main Control Panel
(Side Storage
Incubator)
INSTALLATION (CONTINUED)
BACTRON900 ORIENTATION
BACTRON600 ORIENTATION
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Manometer
Main Control
Panel
Pass Box
Arm Ports
Workspace Chamber
Incubator
Catalyst
Cartridge
Gas and Vacuum
Ports
INSTALLATION (CONTINUED)
BACTRON300 ORIENTATION
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Catalyst
Cartridge
Data Plate
INSTALLATION (CONTINUED)
WORKSPACE CHAMBER ORIENTATION (ALL UNITS)
Figure 1: Workspace Chamber Layout
PASS BOX ORIENTATION
Figure 2: Pass Box Inner Door and Sliding Shelf
The pass box is provided with an integral sliding shelf to facilitate material transfers. Small items
may be introduced into the chamber via the Arm Port Doors and Sleeve Assemblies using proper
sleeve technique.
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INSTALLATION (CONTINUED)
CLEANING
The BACTRON interior was cleaned and disinfected at the factory, but not sterilized. See the
Cleaning procedure in the User Maintenance section for more information.
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CONTROL PANEL OVERVIEW
Figure3: Main Control Panel
Main Control Panel BACTRON300
The main control panel on the BACTRON300 controls the heater and the Over Temperature Limit
System of the workspace chamber incubator. Controls for cycling the pass box atmosphere,
along with the power switch for the BACTRON, and the workstation gas injection light are also
located on this panel.
Main Control Panel BACTRON600
The main control panel on the BACTRON600 controls the heater and the Over Temperature Limit
System of the side storage incubator. Controls for the purging the pass box atmosphere, along
with the power switch for the BACTRON, and the workstation gas injection light are also located on
this panel.
Main Control Panel BACTRON900
The main control panel on the BACTRON900 controls the heater and the Over Temperature Limit
System of the side storage incubator. The controls for the workspace chamber incubator are
located on the workspace control panel (see page 23). The controls for the pass box, along with
the power switch for the BACTRON, and the workstation gas injection light are also located on the
main panel.
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CONTROL PANEL OVERVIEW (CONTINUED)
Main Control Panel Continued
Power Switch
The main power switch on the control panel controls all power to the workstation and must be in the
on ( I ) position before any of the BACTRON’s systems are powered. The switch will illuminate
when on.
Chamber Gas Light
This light indicates that anaerobic mixed gas is being injected into the workspace chamber.
Main Temperature Control and Green Digital Display
Labeled INCUBATOR, this control consists of a green digital display and up and down arrow
buttons used to adjust the incubator’s operating temperature (set point). In its default mode the
display shows the current incubator temperature, accurate to within + 0.1C. The arrow buttons can
also place the unit in its calibration mode. During temperature calibrations, the controls used to
enter temperature offsets to match the displayed temperature to the temperature detected by an
independent reference thermometer.
Heating Activated Light
The clear pilot light located beneath the label HEATING ACTIVATED illuminates whenever the
heating elements are powered and warming the incubator.
Set Over Temperature
This graduated dial sets the incubator’s temperature limit for the Over Temperature Limit backup
system. The OTL System operates independently of the Main Temperature Controller, and
prevents uncontrolled heating of the incubator in the event of a failure of the Main Controller while
in its heating mode. For more details, please see the explanation of the Over Temperature Limit
System in the Operation section’s Theory of Operation.
OTL Light
This pilot light is marked OVERTEMPERATURE ACTIVATED and is directly below the Over
Temperature Limit control. The light will turn on when the OTL System has taken control of the
incubator, and will be accompanied by a buzzer alarm. Under normal operating conditions this light
should not illuminate.
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CONTROL PANEL OVERVIEW (CONTINUED)
Pass Box Doors Light
The clear Doors pilot light indicates that both pass box doors are closed. This light must be on in
order to cycle the pass box.
Anaerobic Light
This is light activates after the completion of a full auto cycle of the pass box Interior.
Aerobic Light
This light activates when the outer pass box door has been opened, exposing the pass box to
aerobic atmosphere. It remains on until the next completion of a full auto cycle.
Pass Box Vacuum Controller and Digital Display
The main control panel vacuum gauge-controller displays the level of vacuum in inches of mercury
in the pass box interior. It controls the application of vacuum to the pass box during the pass box
auto cycle.
Figure 4: Pass Box Vacuum
Controller and Display
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