Control and information center includes central mounted •
touchpad and digital display.
Precise PID logic controls adjust to all temperature •
setpoints and alarm parameters.
High volume, low profile •
cabinets are stackable with
field-reversible doors.
Interior components and •
adjustable shelves are
configured for easy access.
CO2 Control
SANYO proprietary single-beam (IR) CO2 sensor
delivers precise CO
door openings, and auto sampling with no moving
parts.
Ceramic based infrared system that is maintenance free•
Continuous zero calibration is standard.•
control, quick recovery following
2
MCO-40AIC
Active Background Contamination Control
At the base of the plenum, an isolated beam
of high intensity, ozone-free UV light destroys
contaminants in the air and in the water pan, away from
cell cultures, not simply collected in a HEPA filter. Plenum
components isolate UV light to protect cell cultures, while
the UV process continues in the background as programmed
without downtime. Airborne contaminants are eliminated
by an automatic 5 minute UV cycle (programmable 0 - 30
minutes). Trace contaminants which attach to walls, shelves
and plenum components are destroyed by the germicidal
properties of the inCuSaFe™ copper-enriched stainless steel
surfaces.
Humidifying Water Comparison
No exposure to UVFive-minute exposure to
Test results after three months
confirm the efficacy of SANYO
SafeCell™ UV protection on humidifying
water after three months.
Other design factors which help mitigate contamination
include condensation control, inner door gasket design and
triple 0.3 micron filters for vent air and CO
• The SafeCell™ UV air ow plenum promotes temperature
uniformity through the chamber, shaped by natural and
mechanical convection through and around the perforated
shelves with gentle circulation through the plenum for UV
sterilization and warm water humidification.
• Contaminants contained within the distilled water in the
humidity pan are destroyed by UV.
SafeCell™ UV
sensor sampling.
2
• Sterile, humidied air is released from the lower plenum for
vertical convection through and around the perforated shelves
• Interior air motion stops when the door is opened,
minimizing movement of room air into the chamber.
UV Decontamination vs. Heat Sterilization
Independent testing1 confirms that the UV decontamination
technique employed by the SANYO incubator is equally
effective against contamination as conventional high heat
sterilization over a range of +90°C to +140°C. Whenever
overnight or event sterilization of the SANYO incubator
chamber is desired, all interior components are removed for
autoclaving, exposing all interior surfaces to ultraviolet light.
During normal operation when cells are being incubated
within the chamber, the UV lamp is visibly isolated from the
cell culture chamber by a plenum cover over the humidity pan,
permitting UV decontamination of circulated, humidified air
and humidity pan surface water to remain in process without
damaging the cells.
1. A Compara tive Analysis of Ultra violet Light Decontami nation vs. High Heat S terilization in the Ce ll
Culture CO2 Incubator, with the Use of Co pper-Enriched Stainless St eel Construction to Ac hieve
Active Background Contamination Control" H. Busujima; D. Mistry 2007
METHODUVHIGH HEAT
SANYO
TEST RE SULTS, MAX IMUM LOG REDUCTIONS
Bacteria
Overnight
Active Background
Contamination Control
Yeast
Mold
DECONTAMINATION OPTIONS
> 4.5> 4.5> 4.5
> 2.9> 2.9> 2.9
> 2.7> 2.7> 2.7
™
( +140˚ C)(+ 90˚C )
Ideal for Highly Regulated or Sensitive Applications
Interior components and adjustable shelves are
configured for easy access, in situ sterilization and
Model MCO-20AIC is designed for use with a variety of standard
cell culture vessels and protocols. Four adjustable shelves are included,
standard; maximum shelf capacity is 15 shelves.
23
The combination of SANYO's CO2 incubator performance
functions permit use with confidence in high-value cell
protocols among hard-to-grow cell lines, cells highly
sensitive to contamination, ultra-sensitive media and
reagents, or protocols that require a strict isolation and
sterilization between processes. These include but are not
limited to:
1. MCO-20AIC CO2 incubators have received U.S. Food and Drug Administration 510(k) clearance for in vitro fertilization applications in accordance with the FDA Safe
Medical Devices Act of 1990 and the Medical Device Amendments of 1992. Reference: Number K013703. Regulation Number: 21 CFR 884-6120, Assisted Reproduction
Accessories, Regulatory Class II, Product Code 85MOG, October 30, 2001
Page 3
4
5
Control System
Microprocessor Based Controller
SANYO expertise in electronic innovations applies to the
SANYO MCO-20AIC microprocessor control system. All
incubator functions are managed by a fully integrated
controller which acquires and processes information from data
entry, setpoints and alarm parameters.
• P.I.D. Proportional, integral and derivative controls supervise
temperature, and other features for accurate, repeatable
performance.
• A range of setpoint, alarm and programmable inputs are
established through the use of function keys.
• Standard parameters are factory-set for quick start-up, and
all parameters may be changed as required.
• A remote alarm terminal mounted at the rear of the cabinet
can be connected to an external alarm system.
The MCO-20AIC control panel is center mounted in the outer
door for easy access, even when incubators are stacked.
Microprocessor based controls manage all incubator functions
including setpoints, alarm parameters, UV lamp periods,
programming, calibration and diagnostics. Extra-large digital
displays are easy to read. Tactile feedback touchpad data
shift and entry keys simplify operation. When stacked, door
mounted controls remain easily accessible at eye level.
Digital Temperature Display
Multi-function display reads actual
temperature in normal mode
Heat ON Lamp
Indicates when Direct Heat, Air
Jacket elements are powered
UV Lamp
Indicates ultraviolet lamp ON
Door Open Lamp
Warns of inner or outer door ajar
Low Water Lamp
Warns of low water level in
humidity pan
Overtemperature Setpoint
Adjustment
For manual adjustment of high
temperature limit
Set Function Key
Accesses setpoint mode
Digital Shift/Lock Key
Adjusts position in digital
display
Inject Lamp
CO
2
Indicates CO
flow to chamber
2
Numeric Shift Key
Adjusts digits in digital display
Digital CO2 Display Multi-function
display reads actual CO2 density in
normal mode
Calibration Function Key
Accesses calibration
functions and UV lamp periods
Audible Alarm Silence Key
Silences alarm buzzer
Gas Cylinder Supply Indicator
(Optional) Indicates gas supply source
Gas Cylinder Switch Key
(Optional) Allows manual switchover
from primary to secondary gas cylinder
Enter Key
Writes value to the controller
.
SafeCell UV
Active Background
Contamination Control
(5 min. door openings)
SafeCell UV 24HR
Decontamination
Protocol
(24 Hours, total)
Elapsed Time:
Conventional High Heat
Decontamination
(24 Hours, total)
Overtemperature Warning Lamp
Indicates high temperature limit has
been reached normal mode
Start Incubation
PREP: 15 min.
Before sterilization by UV lamp,
remove all attachments (trays,
tray supports, side supports, top
duct, rear duct, humidifying pan,
and pan cover with fan) in the
chamber.
Start Cycle: 24 hours
Follow keypad function code.
UV lamp is on for 24 hours.
15 min.30 min.45 min.1 2
SafeCell UV on:
5 min. after door
opening
Scrubs interior air
flow to destroy
airborne and
humidity pan
contaminants.
3
SafeCell UV on:
5 min. after door
opening
Scrubs interior air
flow to destroy
airborne and
humidity pan
contaminants.
456789101112131415161718192021222324
SafeCell UV on:
5 min. after door
opening
Scrubs interior air
flow to destroy
airborne and
humidity pan
contaminants.
SafeCell UV on:
5 min. after door
opening
Scrubs interior air
flow to destroy
airborne and
humidity pan
contaminants.
SafeCell UV on: 5 min.
(every 12 hours)
Scrubs interior air flow
to destroy airborne
and humidity pan
contaminants.
Finish.
Shelves, humidity pan and plenum
are returned to operating position.
PREP: 15 min.
Remove interior components
sensitive to high heat.
Start Cycle: 90 min.
Interior chamber elevates to high heat.
Sterilize: 14 hours
Interior chamber remains at high heat.
Finish.
Incubator must cool from high heat temperatures to near ambient.
Page 4
6
7
Design & Technology
Infrared CO2 Control
System
The SANYO MCO-20AIC and 40AIC uses a unique
ceramic based infrared sensor system to maintain
precise CO
humidity changes within the incubator chamber. Sensor
stability is especially useful following door openings while
temperature and humidity return to equilibrium.
The ceramic based sensor is maintenance free with no
moving parts and eliminates filament bulbs or electromechanical “chopping” devices.
The CO•
The system allows CO•
0.1% setpoint increments.
Actual CO•
A CO•
convenient confirmation of chamber CO
An optional automatic CO•
See Accessories.
A two-stage regulator from the supply cylinder to the •
incubator is required. See Accessories.
control regardless of temperature and relative
2
sensor automatically calibrates every four hours.
2
control over a range from 0-20% in
2
is displayed on the main control panel.
2
sample port mounted on the incubator front permits
2
switchover system is available.
2
density.
2
SafeCell™ UV System
MCO-20AIC and 40AIC include SafeCell™ UV
contamination control technology based on an
integrated combination of narrow bandwidth, ozone-free
ultraviolet light, ceramic infrared CO
copper-enriched stainless steel alloy interiors and Direct
Heat and Air Jacket™ heating managed by a microprocessor
controller.
These incubators offer the industry's most stable cell culture
environment and are useful for the most critical applications
where continuous contamination control is essential to cell
viability. SANYO SafeCell
significant economic benefits by minimizing interruptions
for decontamination, by improving cell culture growth and
expression under stable, repeatable conditions, and by
minimizing the potential for product loss.
SafeCell•
isolated from cell cultures, that sterilizes conditioned air
and humidity reservoir water to prevent contamination
without affecting cell cultures in vitro.
SafeCell•
molds, spores, yeasts and fungi without costly HEPA filter
air scrubbers which accumulate contaminants in
the chamber air.
High temperature decontamination systems, which •
can actually encourage in vitro growth of heat resistant
thermophilic and hyperthermophilic microorganisms,
are avoided.
InCu SaFe•
to contamination.
™
UV includes a programmable ultraviolet lamp,
™
UV inhibits the growth of mycoplasma, bacteria,
™
interior surfaces provide natural resistance
™
UV Series CO2 incubators offer
control, InCu SaFe™
2
Eliminates Need for HEPA
Filter and Heat Cycle
The MCO-20AIC & MCO-40AIC inhibits the growth
of mycoplasmas, bacteria, molds, spores, yeast
and fungi without costly HEPA filter air scrubbers which
accumulate contaminants in the chamber, or disruptive,
high temperature decontamination schemes which can
actually encourage growth of heat resistant thermophilic and
hyperthermophilic microorganisms in vitro.
As a result, the MCO-20AIC & MCO-40AIC offer a sensitive
yet robust platform for short term, high-throughput drug
discovery projects as well as intermediate and long-term cell
culture investigations.
InCu SaFe™ Construction
for Germicidal Protection
™
SANYO offers exclusive use of InCu SaFe
copper-enriched stainless steel alloy interior
surfaces within a technical design created to eliminate
contamination sources and to mitigate the effect of airborne
contaminates introduced through normal use.
Selected to provide natural germicidal protection without •
rust or corrosion, InCu SaFe
attribute to inhibit the growth of molds, fungi, mycoplasma
and bacteria.
All interior components, including the air management •
plenum, shelf supports, humidity pan and blower wheel
assembly are easily removable without tools if required.
When components are removed, all interior surfaces •
are exposed for conventional wipe down.
Large curve corners and electropolished surfaces are •
easy to clean.
™
expresses a natural germicidal
Pass thru ports accommodate probes or instrumentation •
leads as required for specialized cell culture protocols.
Each chamber includes a port positioned in the interior, rear
wall, upper left, with dual, non-VOC silicone stoppers inside
and outside the cabinet for added protection.
Mycoplasma Survival Results
Chart summarizes test results with four strains of
mycoplasma. Results demonstrate how SANYO InCu SaFe
copper enriched stainless steel alloy offers germicidal
properties of conventional C1100 copper while maintaining
both corrosion-proof and discoloration-resistant properties
of conventional Type 304 stainless steel. Detailed
test results are available from SANYO.
process eliminates contamination without downtime.
At the base of the plenum an isolated beam of high
intensity, ozone-free ultraviolet light destroys contaminants
in the air and in the humidity water reservoir, away from
active cell cultures.
Contaminants trapped within the distilled water pan are •
destroyed by ultraviolet light.
Sterile, humidified air is released from the lower plenum •
for vertical convection through and around the
67
perforated shelves. Interior air motion is suspended when
the door is opened, minimizing movement of room air
contaminants into the chamber.
UV light is isolated by the plenum cover to protect cell cultures•
Airborne contaminants are eliminated by an automatic UV •
cycle that automatically turns ON for a specified period after
each door opening.
Trace contaminants that attach to interior surfaces are •
destroyed by the passive germicidal properties of the
InCu SaFe
™
surfaces.
™
The SafeCell™ UV lamp cycle is factory set for normal use, and can be re-programmed as
desired by entering parameters through the central microprocessor control panel.
SafeCell™ UV
ModeFunction
After Door
Opening
OFFIf UV protection is not desired.
24 HourUseful for overnight decontamination prior to first
UV lamp automatically ON for five minutes after
door is closed decontaminates incoming room air.
use, clinical decontamination protocols between
patients, or following total chamber wipe-out after
maintenance or service.
Page 5
8
9
Design & Technology
Direct Heat and Air Jacket™ Heating System
The patented Direct Heat and Air Jacket™ surrounds the inner
walls with a natural convection airow that converts to radiant
wall heat through thermal conduction to achieve accurate,
uniform and highly responsive temperature control within
the chamber.
The microprocessor controller directs proportional •
distribution of power to independent heating sources
surrounding the chamber.
Arranged in three zones, these sources include the side, •
top and rear walls, the chamber base and the outer door.
Each zone is controlled by the microprocessor which •
manages continuous feedback from the incubator
chamber sensors.
Zone LocationFunctionEnergyMicroprocessor Controller
Main
(Red)
Base
(Yellow)
Front
(Green)
Air Jacket
(White)
Insulation
(Grey)
Side, top and
rear walls
FloorBase heater elevates the humidity reservoir to
Outer doorWarms the inner glass in response to ambient conditions;
Side, top and
rear walls
Side, top and
rear walls, door
Dominant heat sourceVariable
achieve 95%RH at 37°C
eliminates condensation on glass and around the opening
and promotes temperature uniformity
Sealed, surrounds interior chamber with natural air
convection
Promotes energy efficiency, mitigates effect of ambient
temperature uctuations on air jacket
Variable
Variable
– –– –
– –– –
The patented Direct Heat and
Air Jacket™ heating system
distributes proportional energy
to the interior chamber through
a natural convection air jacket
surrounded by a high density
insulation to protect against
ambient temperature fluctuations
Energizes any, all or a
combination of heating elements
as required.
CO2 Incubator Design
Elevated Humidity,
Low Water Level Warning
To avoid cell culture desiccation, the MCO-20AIC & 40AIC
CO
incubator maintains ~95% RH at 37°C.
2
Humidification is achieved by a combined forced-air •
and natural evaporation method enhanced by the Direct
Heat and Air Jacket
optical water level indicator to warn of low water in
the removable humidity pan.
The humidity removes easily for regular cleaning and refill.•
When filled the pan slides into place and the optical •
sensor returns to position and the SafeCell
destroys any contaminants introduced during the process.
Ergonomic Cabinet Design
With reversible inner and outer doors, a single
SANYO incubator offers the industry's most exible
installation options available.
Low profile cabinet with door-mounted control •
panel permits easy access and viewing.
The outer door latch and door heater cable is •
easily switched if a reverse opening is required.
Cabinet knock-outs are pre-drilled and tapped to •
eliminate drilling and to simplify re-mounting of
door hardware.
The outer door closes against a soft, easily •
cleaned magnetic gasket designed to eliminate
ambient air shear across the glass inner door,
minimizing condensation.
A door ajar alarm provides an audible and visual •
warning if the outer door is left open.
™
base heater, and protected by an
™
UV lamp
atmosphere, minimizing condensation and eliminating
moisture traps which can harbor contaminants.
The inner door gasket is a dual durometer extrusion •
(two levels of softness) from closed-cell silicone to
inhibit contamination.
The gasket feather-edge allows the inner glass door •
to close gently against the chamber opening for a
tight peripheral seal.
The entire inner door gasket is removable for cleaning •
and/or replacement if required.
Radiant heat from the outer door, apportioned by the •
microprocessor control as a function of the patented
Direct Heat and Air Jacket
™
, automatically warms the
inner door glass in proportion to total heat demand
and condensation control.
Shelves and Inventory
Management
Inventory management components including
shelves, brackets and shelf supports are formed
from copper-enriched polished stainless steel to inhibit
contamination. All components are removable without tools
for cleaning or autoclaving if required.
Incubator shelves are perforated to permit natural vertical •
air convection through and around labware.
Shelves are easily accessible and can be removed with one •
hand for transfer to a bench or biological safety cabinet.
Shelf brackets slip easily into vertical supports that attach •
to interior chamber walls with clearance sufficient to permit
air circulation against all interior surfaces.
Additional shelves include two brackets. See Accessories.•
Field Reversible Door
Shelves and Inventory Management
Convenient, space efficient inventory
management is simplified through a system
of adjustable, extendable shelves.
• Inventory shelves and brackets are formed from polished copper enriched
stainless steel, removable without tools, and can remain inside the
incubator during the UV decontamination cycle or autoclaved separately if
desired.
• Shelves are perforated to permit natural vertical air convection
through and around labware.
The field-reversible door allows universal installation
using the left-hand hinge (standard) or a right-hand
hinge modification
The outer door includes a universal finger grip •
at each side.
Mounting holes for hinge hardware are pre-drilled •
and capped with easily removable trim plugs.
The door heater cable plugs into the alternate •
connection to complete the change.
Inner Door and Gasket
The inner design is critical to successful contamination
control technique.
The inner gasket body forms an effective thermal transition •
between the ambient air and warm, humidified incubator
Shelves are easily arranged in
1.1”/29mm increments.
Five shelves are supplied
with the MCO-20AIC. Total
incubator capacity is fifteen
shelves.
Optional PC interface, Catalog No. MTR-480 with RS232/RS485 data ports available.
Cabinet Design and Construction
Superstructure, Exterior Cabinet and DoorGalvanized steel exterior, baked-on enamel finish.
Interior and ShelvesCopper-enriched stainless steel.
Inner DoorTempered glass.
InsulationRigid foam polyurethane.
Outer DoorReversible, heated.
Stacking Doubles Capacity in Same Footprint
Access PortSingle opening with interior and exterior 30mm non-VOC silicone stoppers.
Leveling Feet4, Adjustable.
The SANYO CO2 Incubator cabinet is designed for stacking,
allowing one unit to be positioned on top of another, doubling interior volume
without additional floor space.
• The combination of stacking and reversible doors offers the most installation options possible.
• An optional roller base is recommended for stacked installations to permit mobility if required;
see Accessories.
Page 7
Specifications & Accessories
Accessories (SANYO Large Capacity CO2 Incubators)
Single Chamber Model Number
Dual Chamber Model Number
Optional AccessoriesCatalog Number
Automatic CO2 Cylinder Switchover SystemMCO-21GC
CO2 Cylinder Regulator, CGA tting 320MCO-100L
Roller Base. For use in single or stacked installations.MCO-20RB
InCu SaFe™ Shelf and Brackets.
Includes two shelf brackets. Full shelf
Communications Port. Located at rear of chamber.
Connector, cable and software not supplied.
Communications Port. Located at rear of chamber, analog 4-20mA.MCO-420MA
SafeCell™ UV System Kit Narrow-bandwidth
253.7nm lamp and assembly.
SANYO Biomedical products include a broad range of accessories to meet specic applications requirements.
For accessory requirements or options not listed herein, contact SANYO or your authorized SANYO sales representative.
MCO-20AIC
MCO-40AIC
MCO-58ST
MTR-480
Built-In
Product conforms to RoHS
(European Restriction of Hazardous
Substance directives)
SANYO Electric Co.,Ltd., Biomedical Division, Gumma is certified for Quality management
UV U.S. Patent 6255103; Direct Heat and Air Jacket™ U.S. Patent 5519188;
SafeCell
SafeCell™ UV, InCu SaFe™, Direct Heat and Air Jacket™, P.I.D./R™ and Active Background
Contamination Control™, are trademarks of SANYO Electric Biomedical Co., Ltd.