Sanyo MCO-40AIC, MCO-20AIC User Manual

Large Capacity Cell Culture CO

MCO-20AIC MCO-40AIC

Incubators

The industry’s most complete cell culture solution for applications requiring larger ﬂexible volume.

Provides a stable cell culture environment, ranging from 7.6 to 15.2 cu.ft., for speciﬁc temperature and CO continuous contamination control.

control with

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General Features & Beneﬁts

Contamination Control

The SANYO Large Capacity Cell Culture CO2 incubator is designed for a wide array of demanding and highly regulated applications in the

biomedical, pharmaceutical, medical research and clinical laboratory. Representing years of research, development and component testing,

the Cell Culture CO2 incubator incorporates a collective of mutually functional systems and design attributes to offer a holistic solution to cell

culture protocols, from the most sophisticated to more familiar and conventional processes.

Large Capacity CO2 Incubator MCO-20AIC MCO-40AIC

SafeCell™ UV Decontamination Standard Standard

Single Beam, IR CO

Decontamination

Active Background Contamination Control• contamination while cell culture protocols are in process.

The patented SafeCell airflow to destroy airborne and humidity pan contaminants.

Sensor Standard Standard

Temperature and Humidity Control

™

ﬁghts

™

UV system scrubs interior

The patented Direct Heat and Air• setpoint temperature through multiple, variable warming points under microprocessor control.

The humidity pan is easy to ﬁll, • easy to clean; the automatic

™

jacket system manages

optical sensor advises of low

Exclusive InCu SaFe

™

copper-enriched stainless steel

water level.

interior surfaces assure constant germicidal protection.

Cabinet Design

Control and Monitoring

Control and information center includes central mounted • touchpad and digital display.

Precise PID logic controls adjust to all temperature •

setpoints and alarm parameters.

High volume, low proﬁle • cabinets are stackable with ﬁeld-reversible doors.

Interior components and • adjustable shelves are

conﬁgured for easy access.

CO2 Control

SANYO proprietary single-beam (IR) CO2 sensor delivers precise CO door openings, and auto sampling with no moving parts.

Ceramic based infrared system that is maintenance free•

Continuous zero calibration is standard.•

control, quick recovery following

MCO-40AIC

Active Background Contamination Control

At the base of the plenum, an isolated beam

of high intensity, ozone-free UV light destroys contaminants in the air and in the water pan, away from cell cultures, not simply collected in a HEPA ﬁlter. Plenum components isolate UV light to protect cell cultures, while the UV process continues in the background as programmed without downtime. Airborne contaminants are eliminated by an automatic 5 minute UV cycle (programmable 0 - 30 minutes). Trace contaminants which attach to walls, shelves and plenum components are destroyed by the germicidal properties of the inCuSaFe™ copper-enriched stainless steel surfaces.

Humidifying Water Comparison

No exposure to UV Five-minute exposure to

Test results after three months confirm the efficacy of SANYO

SafeCell™ UV protection on humidifying

water after three months.

Other design factors which help mitigate contamination include condensation control, inner door gasket design and triple 0.3 micron ﬁlters for vent air and CO

• The SafeCell™ UV air ow plenum promotes temperature

uniformity through the chamber, shaped by natural and mechanical convection through and around the perforated shelves with gentle circulation through the plenum for UV sterilization and warm water humidiﬁcation.

• Contaminants contained within the distilled water in the

humidity pan are destroyed by UV.

SafeCell™ UV

sensor sampling.

• Sterile, humidied air is released from the lower plenum for

vertical convection through and around the perforated shelves

• Interior air motion stops when the door is opened,

minimizing movement of room air into the chamber.

UV Decontamination vs. Heat Sterilization

Independent testing1 conﬁrms that the UV decontamination

technique employed by the SANYO incubator is equally effective against contamination as conventional high heat sterilization over a range of +90°C to +140°C. Whenever overnight or event sterilization of the SANYO incubator chamber is desired, all interior components are removed for autoclaving, exposing all interior surfaces to ultraviolet light. During normal operation when cells are being incubated within the chamber, the UV lamp is visibly isolated from the cell culture chamber by a plenum cover over the humidity pan, permitting UV decontamination of circulated, humidiﬁed air and humidity pan surface water to remain in process without damaging the cells.

1. A Compara tive Analysis of Ultra violet Light Decontami nation vs. High Heat S terilization in the Ce ll Culture CO2 Incubator, with the Use of Co pper-Enriched Stainless St eel Construction to Ac hieve Active Background Contamination Control" H. Busujima; D. Mistry 2007

METHOD UV HIGH HEAT

SANYO

TEST RE SULTS, MAX IMUM LOG REDUCTIONS

Bacteria

Overnight

Active Background Contamination Control

Yeast

Mold

DECONTAMINATION OPTIONS

> 4.5 > 4.5 > 4.5

> 2.9 > 2.9 > 2.9

> 2.7 > 2.7 > 2.7

™

( +140˚ C) (+ 90˚C )

Ideal for Highly Regulated or Sensitive Applications

Interior components and adjustable shelves are conﬁgured for easy access, in situ sterilization and

exiblearrangementforavarietyofapplications.

Model MCO-20AIC is designed for use with a variety of standard cell culture vessels and protocols. Four adjustable shelves are included, standard; maximum shelf capacity is 15 shelves.

2 3

The combination of SANYO's CO2 incubator performance functions permit use with conﬁdence in high-value cell protocols among hard-to-grow cell lines, cells highly sensitive to contamination, ultra-sensitive media and reagents, or protocols that require a strict isolation and sterilization between processes. These include but are not limited to:

• Stemcellresearch

• Autologoustissueregeneration&regenerativemedicine

• In vitro fertilization

• Genomicandproteomicexpression

• Esotericplantandamphibiancellculture

• Hypersensitiveandtransgeniccellculture

• Lowmediavolumemicroplatework

1. MCO-20AIC CO2 incubators have received U.S. Food and Drug Administration 510(k) clearance for in vitro fertilization applications in accordance with the FDA Safe Medical Devices Act of 1990 and the Medical Device Amendments of 1992. Reference: Number K013703. Regulation Number: 21 CFR 884-6120, Assisted Reproduction Accessories, Regulatory Class II, Product Code 85MOG, October 30, 2001

Control System

Microprocessor Based Controller

SANYO expertise in electronic innovations applies to the

SANYO MCO-20AIC microprocessor control system. All

incubator functions are managed by a fully integrated controller which acquires and processes information from data entry, setpoints and alarm parameters.

• P.I.D. Proportional, integral and derivative controls supervise temperature, and other features for accurate, repeatable performance.

• A range of setpoint, alarm and programmable inputs are

established through the use of function keys.

• Standard parameters are factory-set for quick start-up, and

all parameters may be changed as required.

• A remote alarm terminal mounted at the rear of the cabinet

can be connected to an external alarm system.

The MCO-20AIC control panel is center mounted in the outer

door for easy access, even when incubators are stacked. Microprocessor based controls manage all incubator functions including setpoints, alarm parameters, UV lamp periods, programming, calibration and diagnostics. Extra-large digital displays are easy to read. Tactile feedback touchpad data shift and entry keys simplify operation. When stacked, door mounted controls remain easily accessible at eye level.

Digital Temperature Display

Multi-function display reads actual

temperature in normal mode

Heat ON Lamp

Indicates when Direct Heat, Air

Jacket elements are powered

UV Lamp

Indicates ultraviolet lamp ON

Door Open Lamp

Warns of inner or outer door ajar

Low Water Lamp

Warns of low water level in

humidity pan

Overtemperature Setpoint

Adjustment

For manual adjustment of high

temperature limit

Set Function Key

Accesses setpoint mode

Digital Shift/Lock Key

Adjusts position in digital

display

Inject Lamp

Indicates CO

ﬂow to chamber

Numeric Shift Key

Adjusts digits in digital display

Digital CO2 Display Multi-function

display reads actual CO2 density in normal mode

Calibration Function Key

Accesses calibration functions and UV lamp periods

Audible Alarm Silence Key

Silences alarm buzzer

Gas Cylinder Supply Indicator

(Optional) Indicates gas supply source

Gas Cylinder Switch Key

(Optional) Allows manual switchover from primary to secondary gas cylinder

Enter Key

Writes value to the controller

SafeCell UV

Active Background

Contamination Control

(5 min. door openings)

SafeCell UV 24HR

Decontamination

Protocol

(24 Hours, total)

Elapsed Time:

Conventional High Heat

Decontamination

(24 Hours, total)

Overtemperature Warning Lamp

Indicates high temperature limit has

been reached normal mode

Start Incubation

PREP: 15 min.

Before sterilization by UV lamp, remove all attachments (trays, tray supports, side supports, top duct, rear duct, humidifying pan, and pan cover with fan) in the chamber.

Start Cycle: 24 hours

Follow keypad function code. UV lamp is on for 24 hours.

15 min. 30 min. 45 min. 1 2

SafeCell UV on: 5 min. after door opening