Sanyo MCO-19M UV Installation Manual

INSTRUCTION MANUAL

MCO-19M(UVH)
MCO-19M(UV)

Multi Gas Incubator

MCO-19M

CONTENTS

INTRODUCTION P. 3

PRECAUTIONS FOR SAFE OPERATION P. 4

LABELS ON THE INCUBATOR P. 8
ENVIRONMENTAL CONDITIONS P. 8
INCUBATOR COMPONENTS P. 9

Control panel and keypad P. 12

Remote alarm terminals P. 13
INSTALLATION SITE P. 14
INSTALLATION P. 15
Connection of CO
Connection of N
Connection of gas injection nozzle P. 18
Using the inner door and gastight split doors P. 18
PREVENTING CONTAMINATION P. 19
PRECAUTIONS FOR CULTURES P. 20

Using the unlock key  P. 21

CORRECT OPERATION P. 21
LCD PANEL P. 22
BASIC OPERATIONS ON CONTROL PANEL P. 24
BASIC PARAMETERS
Setting the chamber temperature, CO

Setting the key lock  P. 26
Releasing the key lock  P. 27

Setting the key lock password P. 28
Automatic N
Setting the upper limit alarm temperature P. 32

ALARM PARAMETERS  P. 33

UV LAMP PARAMETERS P. 34
Using the UV lamp P. 34

Precautions when using the UV lamp  P. 35

Setting the UV lamp ON period P. 36
Lighting the UV lamp for 24 hours P. 37
OTHER PARAMETERS
Setting the date, time, and log interval P. 38
Initial settings (LCD/DAQ parameters) P. 40
DISPLAYING THE LOG P. 41
Transferring data P. 43
WATER LEVEL SENSOR P. 44
ROUTINE MAINTENANCE
Cleaning the chamber and inner attachments P. 45
Filling the humidifying pan P. 48
H

DECONTAMINATION P. 49

2O2

H
Precautions when handling H
ALARMS, SAFETY, AND SELF-DIAGNOSIS P. 55
CALIBRATION
Temperature/CO
TROUBLESHOOTING P. 60
DISPOSING OF THE MULTI-GAS INCUBATOR P. 62
AUTOMATIC CO
AUTOMATIC CO
STACKING INCUBATORS P. 72

decontamination P. 51

2O2

gas cylinder P. 16

2

(or O2) gas cylinder P. 17

2

and CO2 density P. 25

2

(or O2) gas cylinder changeover P. 30

2

decontamination reagent P. 54

2O2

calibration P. 58

2/O2

CYLINDER CHANGEOVER P. 67

2

AND O2 DENSITY CALIBRATION P. 69

2

1

CONTENTS

SPECIFICATIONS P. 74
PERFORMANCE P. 75
SAFETY CHECK SHEET P. 76

2

INTRODUCTION

■ Read this manual carefully before using the Product and follow the instructions for safety operation.

■ Sanyo disavows any responsibility for safety if the Product is used for other than the intended use or

used with any procedures other than those given in this manual.

■ Keep this manual in a suitable place so that it can be referred to as necessary.

■ The contents of this manual are subject to change without notice for improvement of performance or

functions.

■ Contact a Sanyo sales representative or agent if any page of the manual is lost or the page order is

incorrect.

■ Contact a Sanyo sales representative or agent if any point in this manual is unclear or if there are any

inaccuracies.

■ No part of this manual may be reproduced in any form without the expressed written permission of

Sanyo.

CAUTION

SANYO guarantees the product under certain warranty conditions. SANYO in no way shall be
responsible for any loss of content or damage of content.

3

PRECAUTIONS FOR SAFE OPERATION

It is imperative that the user complies with this manual as it contains
important safety advice.

Items and procedures are described so that you can use this unit correctly and safely.
If the precautions advised are followed, this will prevent possible injury to the user and
any other person.

Precautions are illustrated in the following way:

WARNING

Failure to observe WARNING signs could result in a hazard to personnel
possibly resulting in serious injury or death.

CAUTION

Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.

Symbol shows;







Be sure to keep this manual in a place accessible to users of this unit.

< Label on the unit >

this symbol means caution.

this symbol means an action is prohibited.

this symbol means an instruction must be followed.

This mark is labeled on the cover in which the electrical components of high voltage
are enclosed to prevent the electric shock.
The cover should be removed by a qualified engineer or a service personnel only.

WARNING

As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity
of the equipment. It is important that you assess the work site to ensure there is suitable and
sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a
safe environment must be considered. These may include atmosphere monitoring and
warning devices.

4

WARNING

Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to

rain water.

Only qualified engineers or service personnel should install the unit. The installation by

unqualified personnel may cause electric shock or fire.

Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from
turning over. If the floor is not strong enough or the installation site is not adequate, this may result

in injury from the unit falling or tipping over.

Never install the unit in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

Never install the unit in a flammable or volatile location. This may cause explosion or fire.

Never install the unit where acid or corrosive gases are present as current leakage or electric

shock may result due to corrosion.

Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not

grounded, it will be necessary to install a ground by qualified engineers.

Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

Connect the unit to a power source as indicated on the rating label attached to the unit. Use

of any other voltage or frequency other than that on the rating label may cause fire or electric shock.

Never store volatile or flammable substances in this unit if the container cannot be sealed. These

may cause explosion or fire.

Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.

This may cause electric shock or injury by accidental contact with moving parts.

Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use

may cause bad effect on your health or environment.

Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any
repair or maintenance of the unit in order to prevent electric shock or injury.

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.

5

WARNING

Ensure you do not inhale or consume medication or aerosols from around the unit at the time of

maintenance. These may be harmful to your health.

Never splash water directly onto the unit as this may cause electric shock or short circuit.

Never put containers with liquid on the unit as this may cause electric shock or short circuit when
the liquid is spilled.

Never bind, process, or step on the power supply cord, or never damage or break the power
supply plug. A broken supply cord or plug may cause fire or electric shock.

Do not use the supply cord if its plug is loose. Such supply cord may cause fire or electric shock.

Never disassemble, repair, or modify the unit yourself. Any such work carried out by an

unauthorized person may result in fire, or electric shock or injury due to a malfunction.

Disconnect the power supply plug if there is something wrong with the unit. Continued

abnormal operation may cause electric shock or fire.

When removing the plug from the power supply outlet, grip the power supply plug, not the cord.

Pulling the cord may result in electric shock or fire by short circuit.

Disconnect the power supply plug before moving the unit. Take care not to damage the power

cord. A damaged cord may cause electric shock or fire.

Disconnect the power plug when the unit is not used for long periods. Keeping the connection

may cause electric shock, current leakage, or fire due to the deterioration of insulation.

If the unit is to be stored unused in an unsupervised area for an extended period, ensure that

children do not have access and that doors cannot be closed completely.

The disposal of the unit should be accomplished by appropriate personnel. Remove doors to

prevent accidents such as suffocation.

Do not put the packing plastic bag within reach of children as suffocation may result.

Use the reagent specified by Sanyo for H

may result in explosion or damage to the Incubator.

When performing H

door. Failure to do so may be harmful to health due to leakage of H

During H
to do so may be harmful to health due to leakage of H

decontamination, plug the access hole with the silicon cap that is provided. Failure

2O2

decontamination, securely close gastight split doors, inner door and outer

2O2

decontamination. Using a different H

2O2

gas.

2O2

gas.

2O2

2O2

solution

6

Use a dedicated power source (a dedicated circuit with a breaker) as indicated on the rating label

attached to the unit. A branched circuit may cause fire resulting from abnormal heating.

Connect the power supply plug to the power source firmly after removing the dust on the plug.

A dusty plug or improper insertion may cause a heat or ignition.

Never store corrosive substances such as acid or alkali in this unit if the container cannot be

sealed. These may cause corrosion of inner components or electric parts.

Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.

Be careful not to tip over the unit during movement to prevent damage or injury.

Prepare a safety check sheet when you request any repair or maintenance for the safety of service

personnel.

Wear rubber gloves when handling the H

result in inflammation of the skin.

H

decontamination can be performed only for the chamber and chamber attachments with

2O2

standard specifications, and not for any other objects.

Perform H

Arranging them in a different way may result in insufficient decontamination.

After H

to wipe off the residual H
decontaminated, and the bottoms of ducts.

2O2

decontamination with the chamber attachments arranged as specified by Sanyo.

2O2

decontamination has been completed, wear rubber gloves and use a non-woven cloth

2O2

CAUTION

reagent. Direct contact with the H

2O2

fluid from the bottom of the chamber, any objects that were

reagent may

2O2

7

LABELS ON THE INCUBATOR

Warning and caution labels are attached to the Incubator. The following table describes the labels.

This label is attached to covers that access high-voltage electrical components to
prevent electric shock. Only a qualified engineer or service personnel should be
allowed to open these covers.

This symbol indicates an ultraviolet light (UV) caution.

This symbol indicates that caution is required. Refer to product documentation for

details.

This symbol indicates a hot surface.

This symbol indicates an earth.

This symbol means “ON” for a power switch.

This symbol means “OFF” for a power switch.

ENVIRONMENTAL CONDITIONS

This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):

■ Indoor use;

■ Altitude up to 2000 m;

■ Ambient temperature 5

■ Maximum relative humidity 80% for temperature up to 31

o

at 40

C;

■ Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage;

■ Other supply voltage fluctuations as stated by the manufacturer;

■ Transient overvoltages according to Installation Categories (Overvoltage Categories) II; For mains

supply the minimum and normal category is II;

■ Pollution degree 2 in accordance with IEC 60664.

o

C to 40oC

o

C decreasing linearly to 50% relative humidity

8

INCUBATOR COMPONENTS

(

)

)

1

18

Handles

Handles

Rear Right Side

3

6

For MCO-19M(UVH)/19M(UV) or
when MCO-19UVS is installed.

12

2

11

13

1524

21

14

9

10(inside

25

4

19

Rear Left Side

5
7

8

16, 17

For MCO-19M(UVH)/19M(UV) or
when MCO-19UVS is installed.

inside

22

23

20

9

1. Outer door: The outer door is held to the frame with a magnetic seal. A door heater is installed in the

door panel. The door opening is reversible. Contact a Sanyo representative or agent to change the door
hinge from left to right or vice versa.

2. Inner door: Open when take the trays out or clean inside.

3. Gastight split door: Made of tempered glass, however avoid excessive impact on the glass.

4. Leveling feet: The leveling feet can be turned to adjust the height. Adjust the feet so that the Incubator

is level.

5. Trays:

tray out.

6. Tray supports: The tray supports can be removed by lifting the front side and pulling toward you.

7. Side supports: The right and left side supports can be removed for disinfection. Refer to page 45.

8. Duct: The duct for the path for circulating air. It is removable.

9. Fan cover: The fan cover serves as the inlet for circulating air. It is removable.

10. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an
autoclave.

11. Sample air outlet: The sample air outlet also functions as an internal gas outlet. Normally, cover this

outlet with the sample air outlet cap.

12. Sample air outlet cap: Always attach this cap except when using the sample air outlet.

13. Door switch: Detects the door opening/closing and stops the circulating fan and electromagnetic

valve for CO

14. Humidifying pan: Fill the humidifying pan with sterile distilled water. Install the pan properly so that it

can be covered with the pan cover.

15. Humidifying pan cover: The cover prevents UV light entering the chamber. When filling the pan, lift

the front side and take out the pan. Refer to page 48 for details.

Set trays at the same level as each gastight split door. Open a inner door when take the

and N2/O2 when door is open. UV lamp is also deactivated by door opening*.

2

16. UV lamp*: This Sanyo UV lamp does not generate ozone. Never look directly at the UV light. For

replacement, contact a Sanyo representative or agent.

17. Water level sensor for humidifying pan: This sensor detects the water level in the humidifying pan.

Refer to page 44 for details.

18. Remote alarm terminals: Refer to page 13.

19. Access port: Place the silicon caps on the port both outside and inside when the port is not being

used.

20. Power switch: This is the main switch for the Incubator. It also functions as an overcurrent breaker.

21. Glow starter*: The glow started if for the UV lamp (model FG-7P)

22. Connecting port A/B for CO

Changeover System is installed, both ports A and B are available. If the MCO-21GC is not installed, only
port A is available. Refer to page 16 for gas cylinder connection. Ensure that the gas pressure is set at

0.03 MPaG (0.3 kgf/cm

2

G, 4.3 psiG). Refer to page 67 for automatic CO2 cylinder changeover.

gas pipe: When the optional MCO-21GC Automatic CO

2

Cylinder

2

10

23. Connecting port A/B for N

the gas pressure is set at 0.05 MPaG (0.5 kgf/cm
gas cylinder changeover.

24. Attachment location for electric key: This is the attachment location for the electric key included in

the optional Component H
decontamination. Refer to the installation procedure for MCO-HL for details.

25. Gas injection nozzle

* MCO-19M(UVH)/19M(UV) or when an optional UV System Kit (MCO-19UVS) is installed.*

2O2

gas pipe: Refer to page 17 for gas cylinder connection. Ensure that

2/O2

Decontamination Kit (MCO-HL). This kit must be attached to perform H2O2

2

G, 7.1 psiG). Refer to page 30 for automatic N2 (or O2)

11

Control panel and keypad

LCD panel

2

1

4

9

1. Upper limit regulator:

This regulator is used to set the upper

temperature limit.

2. OVERHEAT indicator:

This indicator lights when the chamber

temperature reaches the upper limit.

3. MENU Key:

Press this key to access the menu.

4. Contrast knob:

Turn this knob to adjust the contrast of the

LCD.

5. BUZZER Key:
Press this key to silence the buzzer.

Note:

buzzer for an upper limit temperature
alarm.

※ The following optional components must be installed to perform decontamination.
・UV Lamp Add-on Kit (MCO-19UVS) (This is provided as standard equipment for the MCO-19M(UV).)
・H
・H

5

It is not possible to silence the

Decontamination Kit (MCO-HL)

2O2

Generator (MCO-HP)

2O2

6. CE Key:

Press this key to clear the entered value

when entering a setting.

7. Cursor Keys (Up, Down, Left, and Right):

Use these keys to move the cursor on the

LCD panel.

8. ENTER Key:

Press this key to select a menu command.

When entering a setting, pressing this key to

move to the next parameter.

9. Numeric Keys

10. H

Press this key to start H

Refer to page 49 for details.

2O2

Key:

decontamination.

2O2

10

3

7

8

6

12

Remote alarm terminal

The remote alarm terminal is located at the rear right side of the incubator.

The alarm is outputted from this terminal. Contact capacity is DC 30 V, 2 A.

Contact output:

between COM. and N.O. between COM. and N.C.

At normal Open Close

At abnormal Close Open

Note:

The alarm is actuated when the power cord is disconnected from the outlet or the power switch is OFF.

• The remote alarm cannot be stopped by pressing the alarm buzzer stop key (BUZZER) since the remote

alarm is not conjunct with the alarm buzzer stop key (BUZZER).

Remote alarm

terminal

Remote alarm terminal

13

INSTALLATION SITE

For correct operation of the Incubator, install it in a location with the following conditions.

WARNING

When using CO
small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition,
when opening the Incubator doors, do not directly inhale the air in the chamber.

Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO
être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la
porte.

Normal air environment

z

Install the Incubator in an environment with normal air.

Do not expose to direct sunlight

z

Do not install the Incubator in a location where it will be exposed to direct sunlight. If the Incubator is
operated in direct sunlight, performance will be adversely affected.

Separate from heat sources

z

Do not install the Incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves.
Heat will adversely affect the performance of the Incubator.

Ambient temperature at least 5°C lower than set temperature

z

The control temperature of the Incubator is at least 5°C higher than the ambient temperature. For
example, if the chamber is controlled at 37°C, the ambient temperature must normally be no more than
32°C. Do not allow the ambient temperature to become too high.

Strong and level floor

z

Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the Incubator
will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure
that the installation is stable. An unstable surface may result in vibration or noise.

gas for control, make sure that there is adequate ventilation. Using CO

2

de l’air peut s’élever et peut

2

gas in a

2

WARNING

Install the Incubator at a location that can support the weight. If the floor is not strong enough or if the

installation is insufficient, the Incubator may fall over and cause injury.

Always make sure that the floor is strong, even, and level, and that the Incubator will not tip over.

An insufficient installation may result in injury due to water leakage or the Incubator falling over.

Low humidity

z

Select a site with a relative humidity of 80% or lower. Using the Incubator in high humidity may result in
current leakage or electric shock.

WARNING

Do not use the Incubator outdoors. If the Incubator is exposed to rain water, it may result in current

leakage or electric shock.

Never install the Incubator in a moist location, such as near a sink or water line, or where it is
likely to be exposed to water. In addition, do not install it near water or steam pipes. Moisture can

the insulation to deteriorate, which may result in current leakage or electric shock.

cause

No inflammable or corrosive gas

z

Never install the Incubator in a location where it will be exposed to inflammable or corrosive gas. Doing
so may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective
casing, resulting in current leakage or electric shock.

No falling objects

z

Do not install the Incubator in a location where there is the possibility of objects falling from above. Doing
so may result in damage or accident.

14

INSTALLATION

1. Remove the packing tape and clean up.

Remove all the tape that is securing the doors and inner attachments. Open the doors for ventilation. If

the outer panels are dirty, dampen a cloth with a diluted neutral detergent and wipe them. (Using an

undiluted solution may damage the plastic. Follow the diluting instructions for the detergent that is used.)

Wipe off the residual detergent with a wet cloth and then wipe off any moisture.

WARNING

Do not leave plastic wrapping bags within reach of children. If a bag is placed over a child’s head, it

can block the mouth and nose and cause suffocation.

2. Adjust the leveling feet.

Adjust the leveling feet by turning them counterclockwise to level the Incubator. (See the illustration on

the right.)

3. Ground the Incubator.

Ground the Incubator during installation to prevent electric shock in case the insulation is not sufficient. If

there is no ground wire at the location, consult with qualified service personnel.

When a ground must be installed

z

If a grounded 3-pole outlet is not available, then a ground must be installed. Consult with qualified service

personnel.

WARNING

To prevent electric shock, always ground the Incubator. If grounding is not possible, then have a ground

installed by qualified personnel. If the Incubator is not grounded, it may result in electric shock.

Never connect the ground wire to a gas pipe, water pipe, lightning rod, or telephone ground wire.

Doing so may cause electric shock

Installing a ground fault circuit breaker

z

If using the Incubator in a location with moisture or humidity cannot be avoided, then it is recommended

that a ground fault circuit breaker be installed in the power supply circuit (i.e., the power supply at the

Incubator). Have the circuit breaker installed by qualified service personnel.

.

CAUTION

Do not climb on the Incubator or place objects on top of it. Doing so may damage it or cause it to fall

over, resulting in injury. If it is to be stacked, refer to page 72 and stack it securely.

When the Incubator is not in use

z

Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the

chamber is completely dry before closing the doors. Failure to do so may result in damage.

Before moving the Incubator

z

Before moving the Incubator, empty the water from the humidifying pan, disconnect the power supply plug

from the outlet, and make sure that the cord will not be damaged. Failure to do so may result in electric

shock or fire.

15

Connection of CO2 gas cylinder

WARNING

When connecting a gas cylinder to the Incubator, confirm the gas type. Confirm that the connections
are secure and that no gas will escape. Be sure to use the specified pressure. Using an incorrect

gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to escaping

gas.

Install the Incubator in a location with adequate ventilation. If adequate ventilation cannot be

provided, then install an alarm system using

CO2 and O2 densitometers.

1. Use a liquefied CO

gas cylinder (at least 99.5% pure). The siphon (dip tube) type cannot be used.

2

2. Install the Gas Pressure Regulator (MCO-100L, purchased separately) specified by Sanyo on the CO

gas cylinder. Otherwise, use a CO

for the primary pressure, and 0.2 MPaG (2 kgf/cm

gas pressure regulator rated at 25 MPaG (250 kgf/cm2G, 3600 psiG)

2

2

G, 30 psiG) for the secondary pressure.

2

3. Using the gas supply pipe that is provided, connect the CO

gas pressure regulator to the CO2 gas pipe

2

inlet (located at the lower left-hand side on the rear panel of the Incubator).

Note:

If CO

is supplied to multiple CO2 Incubators from a single gas cylinder, a CO2 solid will be formed in the

2

gas pressure regulator. The gas pressure regulator safety valve will operate, and there may an explosive

sound.

When the MCO-21GC is not mounted

Using the gas supply pipe that is provided, connect the CO

cylinder to CO

gas pipe connection port A on the Incubator. After connecting the pipe, check to make

2

gas pressure regulator for the CO2 gas

2

sure that no gas is leaking.

When the MCO-21GC is mounted

Connect a pair of CO

gas cylinders with CO2 gas pressure regulators independently. The gas supply line

2

will be switched automatically. Connect the main cylinder to port A and the reserve cylinder to port B. After

connecting the cylinders, check to make sure that no gas is leaking.

For details on installing the optional automatic CO

z

Cylinder Changeover System (MCO-21GC), refer to

2

the MCO-21GC installation guide. For details on using the MCO-21GC, refer to page 67.

4. Set the CO

pressure increases, the CO

gas on the secondary side to 0.03 MPaG (0.3 kgf/cm2G, 4.3 psiG) for gas injection. As the

2

gas concentration control range will increase. Excessive pressure may

2

cause gas supply lines inside the Incubator to come loose, which may result in gas poisoning or oxygen

deprivation due to the escaping of gas. If gas lines come loose, the Incubator must be repaired.

The gas lines connected to the Incubator will degrade over time. If any deterioration or abnormalities

z

are found during inspection, replace the lines immediately.

16

Connection of N2 (or O2 ) gas cylinder

WARNING

Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are
connected correctly and are not liable to become disconnected. Ensure that the gas pressure is
set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure
may result in leakage of gas. Elevated level of gas can be hazardous to health and may lead to
asphyxiation and risk of death.

This incubator needs N

When the setting of O

When the setting of O

O

density in the atmosphere is about 20%. For the control of O2 density in the chamber, O2 gas is

2

diluted by N

gas when the setting of O2 density is less than that of the atmosphere. On the contrary, O

2

gas is added when the setting of O2 density is more than that of the atmosphere.

1. Install a gas pressure regulator (optional accessory MCO-100L) on N

regulator rated at 25 MPaG (250 kgf/cm

30 psiG) on the secondary side.

2. Using the gas tube provided, connect the pressure regulator to the connecting port A/B for N

pipe located at the rear left side of the incubator. Connect the main cylinder to the connecting port A for

N

gas pipe and connect the sub-cylinder to the connecting port B for N2/O2 gas pipe so that the gas

2/O2

supply can be switched automatically when one gas cylinder is empty. Connect the same gas type to

both connecting port A/B for N

connecting port A/B for N

3. Set the N

or O2 gas pressure on the secondary side to 0.05 MPaG (0.5 kgf/cm2G, 7.1 psiG) (at gas

2

injection). The higher gas pressure makes control range of O

pressure may cause disconnection of internal pipes inside the incubator, which will result in leakage of N

or O

gas into the atmosphere. Elevated level of CO2 gas or O2 gas can be hazardous to health and

2

may lead to asphyxiation and risk of death or fire by gas leakage. The repair of the incubator will be

necessary if the internal pipe is disconnected.

4. Check that no gas is leaking at any point where the pipe connects with the gas regulator or the

incubator.

The gas lines connected to the Incubator will degrade over time. If any deterioration or abnormalities

z

are found during inspection, replace the lines immediately.

or O2 gas depending on the setting of O2 density. The selection is as follows:

2

density is less than 18%: N2 gas

2

density is more than 22%: O2 gas

2

or O2 gas cylinder. Or use a

2

G, 3600 psiG) on the primary side and 0.2 MPaG (2.0 kgf/cm2G,

gas pipe. Never connect N2 gas and O2 gas at the same time to the

2/O2

gas pipe respectively.

2/O2

density wider. In addition, excessive

2

2

2/O2

2

gas

2

WARNING

gas increases the susceptibility of substances to burn. Take care of the handling of flame in a room

O

2

where the incubator is installed.

17



Connection of gas injection nozzle

In the case of control of O

port by using the connecting pipe enclosed (inner diameter; 5 mm, outer diameter; 9 mm, length; 400

mm). This helps faster recovery of humidity after opening of the inner door.

Fill the humidifying pan with sterile distilled water so that the injection nozzle can be under the water level.

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








density in the chamber, connect the gas injection nozzle to the gas injection

2

Gasinjectionnozzle

Using the inner door and gastight split doors

zTo effectively use gastight split doors, set three trays (standard equipment) at the same level as bottom

side of each gastight split door.

















At the same level
as bottom side of
each gastight
split doors

18

PREVENTING CONTAMINATION

To prevent contamination of the chamber, select a suitable installation site.

Avoid locations with high temperatures or humidity.

z

Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in

the air.

Avoid locations with passers-by or drafts.

z

Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry

of microorganisms into the chamber.

If possible, use a cleanroom.

z

To achieve a better culture, it is recommended that a cleanroom be used if one is available.

Use clean containers.

z

The greatest cause of contamination is dirty containers for cultures. Be careful not to get containers or

trays dirty when taking them in and out.

Keep the chamber clean.

z

Wipe off any fingerprints. If water spills from the humidifying pan, or if the doors are left open for a long

time, condensation may form on the inside of the doors. If that occurs, wipe off the condensation with a

dry sterile gauze. In particular, clean and disinfect the chamber if the culture medium is spilled. For details,

refer to Routine Maintenance on page 45.

Use sterile distilled water in the humidifying pan.

z

Always use sterile distilled water in the humidifying pan. Do not use ultrapure water, because it may

contain red rust-like suspended particles.

A water level alarm is displayed in the status display area on the control panel. Quickly refill the water in

the pan when the water level alarm is displayed. Adding low-temperature water will significantly lower the

temperature in the chamber. Clean the humidifying pan once a month.

Keep the Incubator out of direct airflows from air conditioners or fans.

z

Cool airflow from an air conditioner may cause condensation and lead to possible contamination.

19

PRECAUTIONS FOR CULTURES

Leave space between culture containers.

z

Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate

spacing may result in uneven temperature distribution and CO

Do not place harmful materials in the chamber.

z

Never place samples that release acidic, alkali, or corrosive gas in the chamber. Doing so may cause

damage resulting from discoloration or corrosion.

Close the inner door and all gastight split doors.

z

Always close the inner door and all gastight split doors before closing the outer door. Failure to close the

inner door or any gastight split door will adversely affect performance even if the outer door is closed.

Open and close the doors gently.

z

Always open and close the doors gently. Closing the doors forcefully may cause spillage of the culture

medium, incomplete closing, or damage to the gasket. Before opening the inner door, check through the

glass to confirm that the UV lamp is OFF (if the MCO-19M(UVH)/19M(UV) or the optional MCO-19UVS is

installed).

Be careful when closing the outer door.

z

Use the handle when closing the outer door. Holding the door in other places may cause injury by getting

fingers caught in the door. Do not lean on the outer door. Doing so may result in injury from the outer door

coming loose or the Incubator falling over, or it may cause current leakage or electric shock.

Be careful of the inside of the outer door.

z

The inside of the outer door may become hot.

Avoid using excessive force on the inner door.

z

Do not put your hand on the glass, poke it with sharp objects, or apply strong force. Doing so may result

in injury from breaking the glass.

Check the cause of any alarm buzzer.

z

If an alarm buzzer sounds while the Incubator is in use, immediately check the cause of the alarm. For

details on what may cause an alarm buzzer to sound, refer to page 55.

gas concentration.

2

20

Using the Unlock Key

Unlocking when power is interrupted

z

If power is interrupted to the MCO-19M(UVH)/19M(UV) with an MCO-HL installed, the outer door is

electrically locked. To unlock the outer door while the power is interrupted, use the unlock key that is

provided. To lock the outer door again, turn the unlock key to the lock direction while the door is open.

After the door has been locked condition manually, then close the door.

Note:

The outer door cannot be locked by using the unlock key while the door is closed. Lock the door while it is

open. Attempting to turn the unlock key while the door is closed may damage the electric lock system.

WARNING

For the MCO-19M(UVH)/19M(UV) with an MCO-HL installed, the outer door is electrically locked during

decontamination. The chamber is filled with H

during decontamination.



gas during decontamination. Do not unlock the door

2O2

CORRECT OPERATION

Use the following procedure to start trial operation or actual operation of the Incubator.

1. Install the Incubator correctly, referring to Installation on page 15.

2. Remove the packing materials from the chamber and inner attachments. Clean and disinfect the

chamber and inner attachments, referring to Routine Maintenance on page 45.

3. Add approximately 1.5 L of sterile distilled water to the humidifying pan. (Refer to page 48.)

4. Turn ON the power supply switch on the left side of the rear panel of the Incubator.

Note:

The humidity in the Incubator chamber is adjusted to the optimum setting. To prevent condensation on

the surface inside chamber and the inner door, there is a low-temperature area under the humidifying pan

in the bottom of chamber to recondense evaporated moisture. Condensation may occur around the

humidifying pan at the bottom of the chamber, but this does not indicate a problem.

21

LCD PANEL



The following display (called the Top Display) will appear when the power switch is turned ON. The

default temperature is 37.0

and time are preset at the factory. Refer to page 38 to change the date and time.









2






10







1. Display of set value of temperature

The set value of chamber temperature is displayed.

2. Display of current temperature

The current chamber temperature is displayed.

3. Display of set value of CO

The set value of the chamber CO

4. Display of current CO

The current chamber CO

5. Display of current CO

A and B will be displayed if the optional Automatic CO
installed. The port of the CO
Nothing will be displayed if the MCO-21GC is not installed.

6. Display of set value of O

The set value of the chamber O

7. Display of current O2 density

The current chamber O

8. Display of current N

The connecting port A/B for N
reverse video.

1

01 0

1

2

3

4

5

6

7

2

T e

mp 3 7 . 0 o C C O 2 5 . 0 % A B O 2 5 . 0 % A B

３７.０

S t a t u s U V R H P

O K

2

o

C, the default CO2 density is 0% and the default O2 density is 20%. The date

3

11 12 1314151617181920212223242526272829 30 31 32 33 34 35 363738394

５.０

A N D o o r : O p e n

4

density

2

density is displayed.

2

density

2

density is displayed.

2

cylinder

2

Cylinder Changeover System (MCO-21GC) is

2

gas pipe that is currently supplying CO2 will be displayed in reverse video.

2

density

2

density is displayed.

2

density is displayed.

cylinder

2/O2

gas pipe that is currently supplying N2 or CO2 will be displayed in

2/O2

65

５ .

５.０

7

8

0

22





9. Status display field

Various status or alarms are displayed.

・When UV lamp is lit: “UV” is displayed in reverse video. (If the MCO-19M(UVH)/19M(UV) or the optional

MCO-19UVS is installed).

・When humidifying water is low: “RH PAN” is displayed in reverse video and blinks.
・When the door is open: “Door: Open” is displayed in reverse video.

10. Message display field

A message is displayed when fault occurs. The message is displayed alternately in normal characters

and reverse video. Refer to pages from 55 to 57 for alarm details. “OK” is displayed during normal
operation. 







23

BASIC OPERATIONS ON CONTROL PANEL



The following operation are possible through control panel:


z Setting the temperature・・・The chamber temperature can be set (page 25).

z Setting the CO

z Setting the O

z

Setting the key lock・・・Changing the temperature and CO

z Setting alarms・・・The temperature alarm, CO

limit alarm temperature (page 32) can be set.

z Setting UV parameters・・・ON/OFF parameters and the ON period of the UV lamp can be set for the

MCO-19M(UVH)/19M(UV) or when the Incubator is equipped with the optional MCO-19UVS (page 36).

z Setting other parameters・・・The initial settings of the date, time, and log cycle (page 38), and the

LCD display and baud rate (page 40) can be set.

z Displaying the operation log and transferring data・・・A graph of past operation data can be

displayed (page 41) and operation data can be transferred to a PC (page 43).

z H

decontamination・・・H

2O2

performed when the optional H

z Automatic N

30). The CO

System (MCO-21GC) is installed (page 67).

z Setting the standard gas density・・・The standard gas density can be set when the optional

Automatic Standard Gas Calibration Kit (MCO-SG) is installed. (page 69)



density・・・The chamber CO

2

density・・・The chamber O

2

decontamination of the chamber and internal attachments can be

2O2

Generator (MCO-HP) is installed (page 49).

2O2

(or O2) gas cylinder changeover・・・The N

2

gas cylinder can be switched when the optional automatic CO2 Cylinder Changeover

2

density can be set (page 25).

2

density can be set (page 25).

2

density can be disabled (page 26).

2

density alarm, O2 density alarm (page 33) and upper

2

(or O2) gas cylinder can be switched (page

2

24

BASIC PARAMETERS





Setting the chamber temperature, CO



The setting procedure for the chamber temperature, CO

settings:Chamber temperature: 37

The Incubator automatically starts operation using these settings after the power is turned ON.



1. From the Top Display, press the MENU Key to access the menu. Select Set and press the ENTER

Key.




1




2



3



4



5





6



7




2. The Stand-By Setting Display will be displayed. Set the parameters.



1




2



3



4



5





6





The setting ranges of the parameters are as follows:

●Temperature: 0 to 50

●CO

●O



3. Press the MENU Key to display the menu after setting the parameters. Select OK and press the

ENTER Key. The settings will be saved.



Note:

When starting the Incubator for the first time or after not using it for an extended period of time, allow at

least 4 hours for the chamber temperature, humidity, CO

setting the desired chamber temperature at a 0% CO

setting to the desired CO



T e m p 3 7 . 0o C C O 2 5 . 0 %

３７.０

S t a t u s

O K

S t a n d - by S e t t ing
T e m p e r a t u r e

C O 2 D e n s i t y 0 . 0 %
O 2 D e n s i t y 2 0 . 0 %

K e y L o ck 0 (0.Unlock 1 . L o c k)
H i g h L i m i t 5 2 . 0

o

C. To 37.0oC, enter 370.

density: 0 to 20%. To set 5.0%, enter 050.

2

density: 1 to 18 and 22 to 80%. To set 5.0%, enter 050.

2

and O2 density.

2

o

C, CO2 density: 0%, O

５.０

3 7 . 0oC ( 0 . 0oC - 5 0 . 0 o C)

o

and O2 density are given below. (Default

2

density: 20%)

2

( 0 . 0 % - 2 0 . 0 % )
( 1 . 0 % - 1 8 . 0 % )
( 2 2 . 0 % - 8 0 . 0 % )

C

sensor and O2 sensor to become stable after

2

density and a 20% O2 density. Then change the

2

and O2 density

2

O 2 5 . 0 %

M E N U

S e t

５ .

５.０

L o g
T o o l s

M E N U

O K
C a n c e l



25



Setting the key lock



1. To set the key lock, change the value of the key lock parameter from 0 to 1 on the Stand-by Setting
Display and press the ENTER Key. The buzzer will sound briefly and the keys will be locked.




1




2



3



4



5





6

S t a n d - by S e t t ing
T e m p e r a t u r e 3 7 . 0

C O 2 D e n s i t y 5 . 0 %
O 2 D e n s i t y 5 . 0 %

K e y L o ck
H i g h L i m i t 5 2 . 0



2. “Key Lock” will be displayed in reverse video at the right side of “Stand-by Setting”, and changing the

temperature and CO

density settings will be disabled.

2

3. Press the MENU Key to display the menu, select OK and press the ENTER Key. The settings will be

saved.

o

C ( 0 . 0oC - 5 0 . 0 o C)

( 0 . 0 % - 2 0 . 0 % )
( 1 . 0 % - 1 8 . 0 % )
( 2 2 . 0 % - 8 0 . 0 % )

1 ( 0. Unl ock 1 . L o c k)

o

C

M E N U

O K
C a n c e l

26



Releasing the key lock



1. To remove the key lock, change the value of the key lock parameter from 1 to 0 on the Stand-by

Setting Display and press the ENTER Key.




1




2



3



4



5





6

S t a n d - b y S e t t i n g K e y L o c k
T e m p e r a t u r e 3 7 . 0

C O 2 D e n s i t y 5 . 0 %
O 2 D e n s i t y 5 . 0 %

K e y L o ck
H i g h L i m i t 5 2 . 0



2. The cursor will move to the Password Field. Input the 4-digit password (default: 0000) and press the

ENTER Key. When key lock is released, the buzzer will sound briefly, and “Key Lock” will disappear from

the Stand-by Setting Display.





1



2



3



4



5





6

S t a n d - b y S e t t i n g
T e m p e r a t u r e 3 7 . 0

C O 2 D e n s i t y 5 . 0 %
O 2 D e n s i t y 5 . 0 %

K e y L o c k 0
H i g h L i m i t 5 2 . 0



3. Press the MENU Key to display the menu, select OK and press the ENTER Key. The settings will be

saved.



Note: The buzzer will sound for a long time if the wrong password is entered. Enter the correct password.
The password for releasing the key lock must be shared and administered among all users of the

Incubator. The password is set to 0000 when the Incubator is shipped from the factory. The procedure for

changing the password is given next.




o

C ( 0 . 0oC - 5 0 . 0 o C)

( 0 . 0 % - 2 0 . 0 % )
( 1 . 0 % - 1 8 . 0 % )
( 2 2 . 0 % - 8 0 . 0 % )

0 ( 0 . U n l o c k 1 . L o c k )

o

C

K e y L o c k

o

C ( 0 . 0oC - 5 0 . 0 o C)

( 0 . 0 % - 2 0 . 0 % )
( 1 . 0 % - 1 8 . 0 % )
( 2 2 . 0 % - 8 0 . 0 % )

Password

o

C

27



Setting the key lock password



1. From the Top Display, press the MENU Key to display the menu, select Tools, and press the ENTER

Key.




1



2




3



4



5



6




7



2. Select Key Lock PW Setting from the Select Tools Display, press the MENU Key to display the menu,
select OK, and press the ENTER Key.













3. Input the current user password (4 digits), select OK, and press the ENTER Key. (The default user

password is 0000 when the Incubator is shipped from the factory.)













T e m p 3 7 . 0o C C O 2 5 . 0 %

３７.０

S t a t u s

O K

S e l e c t T o o l s ( 1 / 2 )
T e m p / C O 2 C a l i b r a t i o n

A l a r m Se t t i n g
L C D / D A Q S e t t i n g
U V S e t t i n g
K e y L o c k P W S e t t i n g
D a t e T i m e

K e y L o c k P W S e t t i n g

C u r r e n t U s e r P a s s w o r d ＊ ＊ ＊＊

５.０

O 2 5 . 0 %

５ .

５.０

M E N U
S e t
L o g

T o o l s

M E N U

O K

S v C
C a n c e l

28



4. Enter the new user password (4 digits), select OK, and press the ENTER Key.




K e y L o c k P W S e t t i n g





N e w U s e r P a s s w o r d ＊ ＊ ＊＊









5. Enter the new user password again, select OK, and press the ENTER Key to save. After that press the
MENU Key to display the menu, and select Cancel from the menu.




K e y L o c k P W S e t t i n g





N e w U s e r P a s s w o r d ＊＊＊＊




R e E n t e r U s e r P a s s w o r d ＊ ＊＊ ＊







Note:

Be careful not to forget the key lock password. If you have forgotten the password and cannot release the

key lock, contact a Sanyo sales representative or agent.





29



Automatic N2 (or O2) gas cylinder changeover



This incubator switches the gas supply lines when one N

Note: An automatic switcher for the CO

accessory.

Perform the following procedure



1. Connect 2 gas tubes to connecting ports A/B for N
pipes are located on the left back of the Incubator. (Refer to page 9).



2. Connect a N

page 17 for information on connecting the gas cylinders. (“Gas cylinder A” is the N

connected to the connecting port A for N

connected to the connecting port B for N



3. Open the valves of both gas cylinders.



4. The current N







1



2



3




4



5



6



7




5. The incubator detects that there is no more N

chamber does not increase for a while even after opening the N

switches the gas supply line when it detects that there is no more gas. At that time, the following

indications of switching the gas cylinder are provided.



When N2 (or O2) gas gas supply line

switches automatically.

●To silence the buzzer, press the BUZZER Key. The alarm condition is cleared and normal operation

continues.

T e m p 3 7 . 0o C C O 2 5 . 0 % A B O 2 5 . 0 % A B

３７.０

S t a t u s

O K

(or O2) gas cylinder equipped with a gas pressure regulator to each gas tube. Refer to

2

(or O2) gas supply line is displayed on the Top Display.

2



gas supply lines (MCO-21GC) is available as an optional

2

gas pipe, and “gas cylinder B” is the N2 (or O2) gas cylinder

2/O2

gas pipe.)

2/O2

５.０

2

Message display field Display of gas supply

Err02: N2/O2 gas Empty is

displayed.

(or O2) gas cylinder becomes empty.

2

gas pipe. Connecting ports A/B for N2/O2 gas

2/O2

(or O2) gas cylinder

2

Current N2/O2 gas supply line

５ .

５.０

(or O2) gas in a cylinder when the O2 density in the

gas valve in the Incubator. It

2/O2

The character for gas supply line

that is empty blinks.

The gas supply line that is

currently being used is displayed

in reverse video.

30

6. When gas cylinder A is switched to B, remove gas cylinder A and replace it with new one.
Note: Be careful when handling the empty N

cylinder.

●When gas cylinder B becomes empty, the line is switched to gas cylinder A again.

The incubator detects that there is no more N

chamber does not increase for a while even after opening the N

supply line may also be switched for other reasons even though there is gas left in the cylinder: blocking

or restricting of gas tube, reduction of N

cylinder. Always check the gas quantity in the cylinder before disconnecting it.



● Use the following procedure to manual switch the line between N

procedure shows switching the line from N



1. From the Top Display, press the Up Cursor Key for 3 seconds.





1




2



3



4



5




6



7





2. The N




1



2



3




4



5



6




7



(When the line needs to be switched from N

3 seconds.) 



T e m p 3 7 . 0o C C O 2 5 . 0 % A B O 2 5 . 0 % A B

３７.０

S t a t u s

O K

(or O

2

T e m p 3 7 . 0o C C O 2 5 . 0 % A B O 2 5 . 0 % A B

) gas supply line will be switched from cylinder B to A

2

３７.０

S t a t u s

O K

(or O2) gas pressure, or improper opening of N2 (or O2) gas

2

５.０

５.０

(or O2) gas cylinder. Some gas may still be left in the

2

(or O2) gas in a cylinder when the O2 density in the

2

(or O2) gas valve in the Incubator. The

2

(or O2) gas cylinders A and B. This

2

(or O

2

) gas cylinder B to A as an example.

2

５ .

５.０

５ .

５.０

(or O2) gas cylinder A to B, press the Down Cursor Key for

2

31



Setting the upper limit alarm temperature



An upper limit temperature alarm is provided with the Incubator. The alarm temperature can be changed

by using the following procedure.



1. In the Stand-by Setting Display, turn the upper limit regulator on the control panel using a small

screwdriver to set the desired upper limit alarm temperature. The upper limit temperature can be set

between 35 and 51




1




2



3



4



5





6

S t a n d - by S e t t ing
T e m p e r a t u r e 3 7 . 0

C O 2 D e n s i t y 5 . 0 %
O 2 D e n s i t y 5 . 0 %

K e y L o ck 0 ( 0 . U n l o c k 1 . L o c k )
H i g h L i m i t 5 2 . 0



Note:

・Set the upper limit alarm temperature (High Limit) to at least 5

temperature.

・Set the upper limit alarm temperature to at least 50

2. After setting the parameter, press the MENU Key to display the menu, select OK, and press the

ENTER Key. The alarm temperature will be saved.

Refer to the tables of alarms and safety functions for culture operations on page 55 for details.

Note:

・The alarm temperature will be changed at any time by turning the upper limit regulator even if the

Stand-by Setting Display is not displayed.

・Set the upper limit alarm temperature after the chamber reaches the set temperature for operation.




o

C.

o

C ( 0 . 0oC - 5 0 . 0 o C)

( 0 . 0 % - 2 0 . 0 % )
( 1 . 0 % - 1 8 . 0 % )
( 2 2 . 0 % - 8 0 . 0 % )

o

C

o

C higher than the chamber set

o

C when H2O2 decontamination is performed.

32

ALARM PARAMETERS

MEN



1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER

Key.




1



2




3



4



5



6




7

T e m p 3 7 . 0o C C O 2 5 . 0 %

３７.０

S t a t u s

O K



2. Select Alarm Setting from the Select Tools Display, press the MENU Key to display the menu, select
OK, and press the ENTER Key.

01 02 03 04 05 06 07 08 0910 11 12 13 14 15 1617181920212223242526272





S e l e c t T o o l s ( 1 / 2 )



T e m p / C O2 / O 2 C alibration




A l a r m S e t t i n g



L C D / D A Q S e t t i n g



U V S e t t i n g



K e y L o ck P W S etting



D a t e T im e




3. The Alarm Setting Display will appear. The temperature alarm, CO

back time, and door delay can be set on this display. The alarm buzzer can be silenced by pressing the

BUZZER Key. The buzzer will sound again after the specified ring back time if the condition that caused

the alarm continues. The ring back time can be set.

0



1 02 03 04 05 06 07 08 09 10 11 12 13 14 15 1617181920212223242526272829303132 33 34 35 36 37 383940



A l a r m Se t t i n g

1



T e m p A l a r m ± 1 . 0oC ( ±1. 0°C − ± 5 . 0 ° C ）



3



C O 2 A l a r m ± 1 . 0 % ( ± 0 . 5 % −±5 . 0 % ）



O 2 A l a r m ± 1 . 0 % ( ± 0 . 5 % − ± 5 . 0 % ）

4



A l a r m De l a y 15min (0-15min)

5



R i n g Ba c k 30min (0.OFF 1− 9 9 m i n ）



D o o r De l a y 2min (1-30min)



6



・Temperature alarm: ±1.0 to ±5.0
・CO

alarm, O2 alarm: ±0.5 to ±5.0% (Default: ±1.0%)

2

・Alarm delay: 0 to 15 minutes (Default: 15 minutes)
・Ring back time: 1 to 99 minutes, or OFF (Default: 30 minutes)
・Door alarm delay: 1 to 30 minutes (Default: 2 minutes)

５.０

o

C (Default: ±1.0oC)

O 2 5 . 0 %

５ .

５.０

U

OK

Cancel

2303132 33 34 35 36 37 383940

8

alarm, O2 alarm, alarm delay, ring

2

M E N U
S e t
L o g

T o o l s

M E N U

O K

S v c
C a n c e l

M E N U

O K

C a n c e l

33

UV LAMP PARAMETERS



A UV lamp is used with the MCO-19M(UVH)/MCO-19M(UV) or the MCO-19M with an MCO-19UVS UV

Lamp Expansion Kit installed. Use the following procedures to make the settings.



Using the UV lamp



A UV lamp is located inside the duct to sterilize the water in the humidifying pan and the air circulating in

the chamber. Observe the following points to use the UV lamp correctly.

When all chamber attachments are installed correctly, only the inside of the duct and the inside of the

z

humidifying pan cover are exposed to UV light.

Correctly install all of the chamber attachments when starting a cell culture, and never turn ON the UV

z

lamp when the humidifying pan cover is removed.

Always install the humidifying pan cover even when using the Incubator without turning ON the UV

z

lamp. Leaving the cover uninstalled will affect the chamber temperature distribution and the humidity

recovery performance.

The UV lamp stays lit for a preset period of time after the outer door is closed. The default setting is for

z

five minutes.

If the outer door is not opened for at least 12 consecutive hours, the UV lamp will light for the preset

z

time period every 12 hours.

The recommended replacement time for the UV lamp (i.e., when the UV output ratio drops to 60% to

z

70% of its initial value) is when the accumulated ON time reaches 1,000 hours. When the accumulated

ON time reaches approximately 1,000 hours, the “UV” will flash in the status display field on the LCD

panel (when the UV lamp is not lit). It is recommended that the UV lamp be quickly replaced at this point.

Consult a Sanyo sales representative or agent for information on replacing the UV lamp.

If the UV lamp burns out, Err18:UV Lamp Abnormal will be displayed in the message display field. If

z

this occurs, replace the UV lamp. When replacing the UV lamp, replace the glow starter (type FG-7P) at

the same time. Consult a Sanyo sales representative or agent for information on replacing the UV lamp.

If the UV lamp burns out (Err18:UV Lamp Abnormal will be displayed in the message display field), it

z

will not be possible to perform H



decontamination. Replace the UV lamp and the glow starter.

2O2

34



Precautions when using the UV lamp



WARNING

Do not look directly at UV light. UV light is harmful to the eyes.

Always use the humidifying pan and humidifying pan cover.

z

The humidifying pan and cover prevent UV light from escaping. Always use them even when not

humidifying. To check whether the UV lamp is lit, open the outer door and then press the door switch with

the inner door still closed. Visible blue light can be confirmed from the front of the humidifying pan cover.

UV light is harmful to the eyes, so do not light the UV lamp when the inner door or humidifying pan cover

is open.

Be careful when handling the UV lamp.

z

There is a UV lamp inside of the chamber duct. Be careful not to damage it when installing or removing

chamber attachments, the humidifying pan, or the H




Generator.

2O2

35

MEN



Setting the UV lamp ON period


Use the following procedure to change the setting of the UV lamp ON period.



1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER

Key.




1



2




3



4



5



6




7



2. Select UV Setting from the Select Tools Display, press the MENU Key to display the menu, select OK,

and press the ENTER Key.
















3. The UV Setting Display will appear. Set the UV Timer for the UV lamp ON period.




1




3




4



5





6



4. Press the MENU Key to display the menu, select OK, and press the ENTER Key. The parameter will

be saved.




T e m p 3 7 . 0o C C O 2 5 . 0 %

３７.０

S t a t u s

O K

01 02 03 04 05 06 07 08 0910 11 12 13 14 15 1617181920212223242526272

S e l e c t T o o l s ( 1 / 2 )
T e m p / C O2 / O 2 C alibration

A l a r m Se t t i n g
L C D / D A Q S e t t i n g
U V S e t t i n g
K e y L o ck P W S etting
D a t e T im e

U V S e t t i n g
U V T i m er 5 mi n ( 0 - 3 0 mi n )

U V L i f e 0 % (100%=1000h)
U V T i m e r E x t + 0 %

U V 2 4 h M o d e 0 ( 0 . O F F 1 . O N )

５.０

O 2 5 . 0 %

５ .

５.０

U

OK

Cancel

8

9303132 33 34 35 36 37 383940

M E N U
S e t
L o g

T o o l s

M E N U

O K

S v c
C a n c e l

36



• The UV timer can be set from 0 to 30 min. The default setting is for 5 min. When the UV timer is set to
zero, the UV lamp will not light.

• If the outer door is opened while the UV lamp is lit, the lamp will turn OFF. Then, when the door is
closed, the lamp will light for the preset period of time.

• If the UV lamp ON time is set for longer than 10 minutes or if only the outer door is repeated opened
and closed, condensation may occur in the chamber and may affect temperature distribution. It will also
shorten the service life of the UV lamp.

• When replacing the UV lamp, consult with a Sanyo representative or agent.

Note: To compensate for the drop in UV ray output along with increased accumulated ON time of UV

lamp, the Incubator automatically extends the UV lamp ON time according to the accumulated ON time.

The automatically extended time is displayed as a percentage under UV Timer Ext. in the UV Setting
Screen.



Lighting the UV lamp for 24 hours

If the chamber has been contaminated by dirt or by spilling the medium, use the following procedure to
decontaminate the chamber by lighting the UV lamp for 24 hours.



1. Remove all attachments from the chamber, including the trays, tray supports, side supports, fan cover,

duct, fan, humidifying pan, humidifying pan cover, N
attachments in an autoclave or with disinfectant alcohol.

2. Clean and wipe off inside the chamber with disinfectant alcohol.

3. Set the CO




U V S e t t i n g

1



U V T i m er 5 min (0-30min)

3



U V L i f e 0 % (100%=1000h)




U V T i m e r E x t + 0 %

4



5



U V 2 4 h M o d e 1 (0.OFF 1.ON)



6




4. Press the MENU Key to display the menu, select OK, and press the ENTER Key. The parameter will

be saved.

・This procedure must be preformed only with the outer door closed and the UV lamp turned OFF.
・The UV lamp will light continuously for 24 hours after this parameter is set. The setting is canceled if the

outer door is opened. Perform the above procedure to set the 24-hours lighting mode again if you open
the door.

・Install the attachments after completion of the 24-hour lighting mode.

Note: Set the upper limit alarm temperature to at least 10

when using the 24-hour lighting mode of UV lamp. The 24-hour lighting mode may cause an automatic

chamber temperature alarm because the temperature of the chamber will increase.

density to 0% and O2 density to 20%. Set the UV 24h Mode to 1 on the UV Setting Display.

2

or O2 gas injection nozzle, and. Clean all the

2

o

C higher than the chamber set temperature

37

OTHER PARAMETERS



Setting the date, time, and log interval



1. Press the MENU Key from the Top Display to display the menu, select Tools and press the ENTER Key.




1



2




3



4



5



6




7



2. Select Date Time from the Select Tools Display, press the MENU Key to display the menu, select OK,

and press the ENTER Key.

































T e m p 3 7 . 0o C C O 2 5 . 0 %

３７.０

S t a t u s

O K

S e l e c t T o o l s ( 1 / 2 )
T e m p / C O 2 C a l i b r a t i o n

A l a r m Se t t i n g
L C D / D A Q S e t t i n g
U V S e t t i n g
K e y L o ck P W S etting
D a t e T i m e

O 2 5 . 0 %

５ .

５.０

５.０

M E N U
S e t
L o g

T o o l s

M E N U

O K

S v c
C a n c e l

38





3. The Date Time Display will appear. Set the date, time and log interval.




D a t e T i m e

1



3



D a t e 1 0 / 1 0 / 0 1 ( Y Y / M M / D D )




4



5




6



・Entering the date
Example for October 1, 2010: Enter 101001 in the Date Field.

・Entering the time

Example for 10:05:00: Enter 100500 in the Time Field.

・Entering the log interval

Example for 6 minutes: Enter 6 in Log Interval Field.

Note:

■ The default is 6 minutes.

■ The log interval can be set to between 2 and 30 minutes.

■ Relation between the log interval and the period that can be saved

1: Log interval of 2 minutes -- About 5 days

2: Log interval of 6 minutes -- About 14 days

3: Log interval of 30 minutes -- About 70 days

For the data beyond the above period, the older data is deleted to save the newer data.




T i m e 1 0 : 0 5 : 0 0 ( h h ： m m ：s s )

L o g I n t e r v a l 6 m i n ( 2 - 3 0 m i n )

39





Initial settings (LCD/DAQ parameters)



1. Select LCD/DAQ Setting from the Select Tools Display, press the MENU Key to display the menu,
select OK, and press the ENTER Key.





S e l e c t T o o l s ( 1 / 2 )




T e m p / C O 2 C a l i b r a t i o n



A l a r m Se t t i n g



L C D / D A Q S e t t i n g



U V S e t t i n g



K e y L o ck P W S etting




D a t e T i m e




2. The LCD/DAQ Setting Display will appear. Set the initial setting for each parameter as necessary.




L C D / D A Q S e t t i n g

1



L C D B a c k C o l o r 1 ( 1 . B l u e 2 . W h i t e )

3



D A Q S p ee d 0 ( 0. 2400 2. 96 0 0 )




D A Q I D 0 ( 0 . O F F 1 - 2 5 0 )

4



D A Q M o d e 0 ( 0 . L o c a l 1 . R e m o t e )

5



R e m o t e A l a r m 1 ( 0 . O F F 1 . A c t i v e )



6




・LCD Back Color: Setting of the background color (1: Blue, 2: White)



Note:

DAQ is an external monitoring system of the chamber status. It is necessary to set the DAQ speed, DAQ

ID, and DAQ mode to use the optional communications software. Communications software is an
optional accessory. Contact a Sanyo sales representative or agent for details.




M E N U

O K

S v c
C a n c e l

40

DISPLAYING THE LOG



A log of the past chamber temperatures, CO



1. Press the MENU Key from the Top Display to display the menu, select Log and press the ENTER Key.





1




2



3



4



5




6



7

T e m p 3 7 . 0o C C O 2 5 . 0 %

３７.０

S t a t u s

O K

densities and O2 densities can be displayed on a graph.

2

５.０

O 2 5 . 0 %

５ .

５.０

M E N U
S e t

L o g

T o o l s

41





2. The log will be displayed with dots. Press the Up Cursor Key and Down Cursor Key to switch between

the temperature, CO

scroll the displayed data (Left Cursor Key: older data, Right Cursor Key: newer data).




Temperature



5 0 ° C 2 0 10/10/01 T e mp



1



3




4



5





0 1 2 24

6







CO

density

2



2 0 % 2 0 10/10/01 C O 2



1



3




4



5





6

1 2 24

0







O2 density



9 0 % 2 0 10/10/01 O 2



1



3




4



5





6

1 2 24

0







density and O2 density displays. Press the Left Cursor Key and Right Cursor Key to

2

Switched with Up and Down Cursor

Switched with Up and Down Cursor

42



Transferring data



Use the following procedure to transfer the log data to a PC.


1. To transfer the log data for one day, press the MENU Key to display the menu, select PC 1D, and
press the ENTER Key. To transfer all of the log data, select PC All and press the ENTER Key.




5 0 ° C 2 0 10/10/01 T e mp



1



3




4



5





6



2. A Progress Display will appear. On a HyperTerminal on PC, specify a transfer, text capture, and the

name of the file to save. Use TXT or CSY as the file name extension. Press the MENU Key to display the

menu, select Start, and press the ENTER Key.

The transfer will be started. “Finished” will be displayed when the transfer has been completed. Select

Cancel from the menu after the transfer has been completed and press the ENTER Key.




1



3





4



5




6





Setting in PC side for transmission of log data (For Windows 2000 and Windows XP)

1. From the Windows Start Button, select Program

HyperTerminal to start the HyperTerminal. (If the HyperTerminal is not available from the Start Menu,

execute the following file: C:¥Program Files¥Windows NT¥hypertrm.exe.)

2. In the HyperTerminal Window, set a new connection, the name (for example, Sanyo), connection

settings, method of connection, COM1, properties of COM1, and port.

Baud rate: 9,600, Data bits: 8, Parity: None, Stop bits: 1, Flow control: Xon/Xoff.

(The communications condition of the Incubator will be set automatically to the above settings when the

Progress Display appears.)

Note: An optional RS232C/RS485 Interface MTR-480 (RS-232C/RS-485) is required to transfer data.

0

1 2 24

P r o g r e ss

S e n d l og d a t a to PC.

L o g D a t a 2 0 1 0 / 1 0 / 0 1

F i n i s h e d .

M E N U

P C 1 D

-

Accessories

P C A l l
C l e a r
C a n c e l

M E N U

S t a r t

C a n c e l

-

Communications

-

43

WATER LEVEL SENSOR

This Incubator is equipped with a water level sensor for the humidifying pan. The sensor is set

automatically when the humidifying pan is installed. Take care not to damage the sensor when removing

or installing the humidifying pan.

When the humidifying pan is removed (side view)

When the humidifying pan is installed (side view)

Note: Make sure that the humidifying pan is all the way to the back and that the sensor comes down

when you install the humidifying pan.

Note:

• Lift the sensor before installing the humidifying pan if the sensor is in the lower position after

maintenance.

• When installing the humidifying pan, make sure that the pan is set properly and that sensor comes down

into the pan. “RH PAN” will be displayed in reverse video in the status display field on LCD panel if the

sensor does not come down completely. If necessary, set the pan again in the proper location.

• The sensor detects the water level every 30 minutes and just after the outer door is closed. It takes

several seconds to detect the water level. Therefore, “RH PAN” may displayed in reverse video several

times in the status display field on LCD panel after the outer door is closed even when the humidifying pan

is full.

Pan

Pan

Sensor

Sensor

CAUTION

Foreign particles on the water surface can adhere to the water level sensor and fittings by capillary action

because the sensor is always in the water. The adhered foreign particles degrade sensor performance

and “RH PAN” may displayed in reverse video in the status display field on LCD panel even though there

is sufficient water in the humidifying pan. Be sure to wipe OFF any dirt on the water level sensor with

disinfectant alcohol whenever you change the humidifying water. When cleaning the sensor, take care

not to apply excessive force to the lead wires.

44

ROUTINE MAINTENANCE

t

Cleaning the chamber and inner attachments

When using the Incubator for the first time or when performing H

Chamber Attachments on the following page and remove and clean the inner attachments.

WARNING

Before performing any repairs or maintenance, turn OFF the power switch and unplug the Incubator.

Failure to do say may result in electric shock or injury.

Be careful not to inhale chemicals, vapors or aerosols when cleaning the Incubator. Doing so may

be harmful to health.

decontamination, refer to Removing

2O2

CAUTION

Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may

result in cuts or abrasions from sharp edges or corners.

Note:

Be careful not to damage the humidifying pan water level sensor or the UV lamp in the chamber duct (if

the MCO-19M(UVH)/19M(UV) or the optional MCO-19UVS is installed).

Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause

discoloration, corrosion, or rusting.

1. Close the valve of the CO

2. Open the outer and inner doors and pull out the all trays. (See Fig. 1.)
Note: Open the inner door after closing all gastight split doors.

3. Lift the front of the tray supports and pull them out. (See Fig. 2.)

4. Lift the humidifying pan cover off from the pins on the rear side. (See Fig. 3.)

and N2/O2 gas cylinder and turn off the power to the incubator.

2

Tray suppor

Fig. 1 Fig. 2 Fig. 3

Humidifying pan cover

45

5. Pull out the N

6. Pull out the humidifying pan. (See Fig. 5.)

7. Loosen the two screws securing the fan cover and take off the fan cover. (See Fig. 6.)

gas injection nozzle and N2/O2 gas injection nozzle tube. (See Fig. 4.)

2/O2

Gas injection nozzle tube

8. Lift the duct and remove it from the pins on the rear side. (See Fig. 7.)

9. Remove the chamber circulation fan by pulling out the central spring and then by pulling out the fan.

(See Fig. 8.)

Gas injection nozzle

Fig. 4

Humidifying pan

Fig. 5

Fan cover

Fig. 6

10. Remove the screw securing the clamp for the side support and remove the clamp. (See Fig. 9.)

11. Lift the tray brace off of the pins. (See Fig. 10.)

Duct

Clamp

Fig. 7

Side

support

Fig. 9

Fan

Fig. 8

Fig. 10

46

12. Clean all the attachments with a diluted neutral detergent, and then rinse them thoroughly with

distilled water.

13. Wipe the trays, the inner attachments such as the chamber circulation fan, and the chamber sides
with sterilizing alcohol. Be careful not to leave any residual alcohol.

14. Wipe the water level sensor with sterilizing alcohol. Be careful not to leave any residual alcohol. When
cleaning the sensor, take care not to apply excessive force to the lead wires.

15. To reinstall all the attachments, perform the procedure in reverse order from step 14.

Note:

• When installing the fan, insert it securely on the motor shaft (See Fig.11). Lightly turn the fan manually

to make sure that it does not strike the rear panel. Improper insertion may cause poor performance.

• As shown in Fig. 12, set the tray with only the front edge bent down. If the tray is set in the wrong

direction, it may not be level and may become unstable.

Spring

Fan

① ②

Fig. 11

Bent down

Fig. 12

47

Filling the humidifying pan

Use the following procedure to fill the humidifying pan or to replace the water.

1. Lift the humidifying pan cover. (See Fig. 1.)

2. Pull the humidifying pan forward. (See Fig. 2.)

3. Dispose of the remaining water in the pan and

clean the pan with a neutral dishwashing detergent.

Then rinse it thoroughly with distilled water and wipe

it with sterilizing alcohol.

4. Wipe all moisture from the bottom of the chamber.

5. Return the pan to the chamber and add sterile

distilled water (approx. 1.5 L, preheated to 37°C).

(See Fig. 3.)

6. Set the pan with the inner side flush against the

back, and replace the cover. Close all gastight split

doors, inner door and outer door, and confirm that

RH PAN is not displayed in reverse video in the

status display area.

Note:

• Preheat to 37°C the water to be added to the

humidifying pan. Adding low-temperature water will

lower the temperature and humidity in the chamber.

• If the water level alarm indication “RH PAN” is

displayed, use the above procedure to replenish the

water.

Fig. 1

Fig. 2

CAUTION

When refilling the water in the humidifying pan,

always wipe off any dirt from the water level
sensor with sterilizing alcohol. While doing that,

be careful not to apply excessive force to the

sensor lead wire.

Fig. 3

48

H2O2 DECONTAMINATION



When the chamber has been contaminated, or when cleaning the chamber prior to starting a culture,

H

decontamination can be performed using the H2O2 Generator.

2O2

The following products must be purchased separately in order to perform H

z

performing H

decontamination, check to make sure that these are correctly installed.

2O2

• UV Lamp Expansion Kit (MCO-19UVS) (Not required for the MCO-19M(UV).)

• H

• H

Decontamination Kit (MCO-HL)

2O2

Generator (MCO-HP)

2O2

The H

z

decontamination function decontaminates the chamber and the inner attachments. Do not

2O2

place any objects other than the specified attachments in the chamber during decontamination.

Before performing H

z

decontamination, clean the chamber and the inner attachments with sterilizing

2O2

alcohol.

The chamber temperature during H

z

decontamination reaches 45°C. Before performing H2O2

2O2

decontamination, set the upper limit temperature alarm to 50°C or higher.

For the H

z

reagent, use one bottle of the H2O2 Decontamination Reagent specified by Sanyo

2O2

(MCO-H202).

After decontamination, dilute the residual H

z

Decontamination Reagent in the H2O2 Generator with a

2O2

large quantity of water and dispose of it, and rinse with distilled water. (Do not wash either the inside or

outside of the H

Generator with alcohol.)

2O2

decontamination. Before

2O2

WARNING

Use the reagent specified by Sanyo for H2O2 decontamination. Using a different H

solution may

2O2

cause explosion or damage to the Incubator, or insufficient decontamination.

Do not use chemicals other than the H

to the H

Generator.

2O2

reagent, such as alcohol. Doing so may result in damage

2O2

WARNING

When performing H2O

door are securely closed. During H

provided. Failure to do so may be harmful to health due to leakage of H

decontamination, make sure that gastight split doors, inner door and outer

2

decontamination, plug the access hole with the silicon cap that is

2O2

gas.

2O2

CAUTION

H2O2 decontamination can be performed only for the chamber and chamber attachments with standard

specifications, and not for any other objects.

49

CAUTION

Perform H2O2 decontamination with the chamber attachments arranged as specified by Sanyo.

Arranging them in a different way may result in insufficient decontamination.

CAUTION

Wear rubber gloves when handling the H2O2 reagent. Direct contact with the H

in inflammation of the skin.

CAUTION

After H2O2 decontamination has been completed, residual H2O2 solution will remain on the bottom of the

chamber, the H

gloves, wipe it off with a non-woven cloth. Failure to do so may result in a deficient culture.



Generator, and the bottom of the duct. Wearing protective glasses and rubber

2O2

reagent may result

2O2

50

t

H2O2 decontamination

Use the following procedure to perform H

1. Take out all trays, tray supports, side supports, the humidifying pan cover, the N

nozzle, the N

or O2 gas injection nozzle tube, the humidifying pan, the fan cover, and the duct from the

2

chamber. Dispose of the water in the humidifying pan, wipe the inside walls of the chamber with a gauze
containing water or alcohol for sterilization.

2. Attach the duct and side supports, and set the tray supports in the 2nd, 5th, 9th holes from the top of

the side supports. Then set the trays.

3. Pour one bottle of H

pins on the H

Generator in the 2 holes on the lower left side of the duct (Fig. 1).

2O2

Decontamination Reagent (MCO-H2O2) into the H2O2 Generator. Set the two

2O2

4. Connect the H

Generator and connector on the bottom right of the far side of the chamber with the

2O2

enclosed cable. Be sure to keep the connector cap (Fig. 1).

5. Set the humidifying pan cover, humidifying pan, fan cover, the N

or O

gas injection nozzle tube as shown in Fig. 2, and close the inner door and outer door .

2

Note: The trays included as accessory are designed to be appropriate for decontamination. If Half Trays

(MCO-25ST, optional) or Trays for previous models are used, decontamination may not be sufficiently
effective.

Front

H2O2 Generator

Ultrasound oscillator

Front

2O2

UV lamp

decontamination using the H2O2 Generator (MCO-HP).

or O2 gas injection

2

or O2 gas injection nozzle and the N2

2

Fron

Connector

Humidifying
pan cover

Fan cover

N2 or O2 gas
injection nozzle
N

or O2 gas

2

injection nozzle tube

Humidifying pan

H

2O2

Generator

Fig. 1

Fig. 2

51

6. Press the H

H 2 O 2 D e c o n t a m ination

1

3

4

5

6

7. If the system is normal, the following display will appear. (If the system is not normal, refer to Table 2
on page 57.) Select OK and press the ENTER Key to start H

be performed automatically to step 10. The buzzer will sound when decontamination has been completed

(It takes about 1 hour 40 minutes after warming-up).

Note: The outer door will be locked with an electric lock for safety until the decontamination has been
completed.

H 2 O 2 D ec o n t a m ination

1

3

4

5

6

8. During the H
Note: H

2O2

H 2 O 2 D e c o n UV Resolve S t e p:4

1

2

3

4

5

6

7

Key for 3 seconds. The system check will start.

2O2

H 2 O 2 D e c o n S y s tem C h e c k ・ ・・ ・ ・

decontamination. Decontamination will

2O2

M E N U

R e a d y t o S t a rt?

mist generation, “H2O2 Decon” will flash at the top left corner of the screen.

2O2

O K

C a n c e l

mist generates from the H2O2 Generator and then quickly changes to a gas.

７min

９０min

T e m p : 4 5 . 0
D o o r : L o c k e d

℃

52

9. After completion of H

resolution by UV light will start.

1

2

3

4

5

6

7

H 2 O 2 D e c o n U V R e s o l v e S t e p:7

０min

10. After H

gas resolution, H2O2 decontamination has been completed. The following display will

2O2

appear. Open the outer door and disconnect the cable of H

chamber. Then take out the H

Note: Always put on protection glasses and rubber gloves when performing decontamination.

1

2

3

4

5

6

7

H 2 O 2 D e c o n t a m i n a t i o n

D e c o n F i n i s h e d .

P l e a s e R e m o v e H 2 O 2 U n

11. The following display will appear. Press the MENU Key to display the menu, select OK, and press the

ENTER Key to return to the Top Display.

1

2

3

4

5

6

7

H 2 O 2 D e c o n t a m i n a t i o n S t e p:8

D e c o n F i n i s h e d .

P U S H M E N U O K

mist generation, “UV Resolve” will flash at the top of the screen, and H2O2 gas

2O2

８９min

T e m p : 4 5 . 0
D o o r : L o c k e d

Generator from the connector in the

2O2

Generator.

2O2

S t e p:8

T e m p : 4 5 . 0

i t D o o r : U n l o c k

T e m p : 4 5 . 0 ℃
D o o r : U n l o c k

℃

℃

53

12. Dilute the remaining H

it. Rinse and wash the H

environment outside of the chamber.

Note: After H

13. After H

bottom part of the H

less), so put on protective glasses and rubber gloves and wipe it up with a non-woven cloth.

14. Ventilate the chamber sufficiently and place all the attachments back into the chamber.
Note: Be sure to attach the connector cap.

CAUTION



The electric lock will remained locked if power fails during H2O2 decontamination. After the power comes

back ON, the H

decontamination again because the decontamination will be incomplete.

decontamination, cover the connector on the chamber side with the connector cap.

2O2

decontamination, surplus H2O2 liquid will remain at the bottom of the chamber and in the

2O2

2O2

gas resolution process will start execution and finish automatically. Execute the

2O2

reagent in the H2O2 Generator with a large volume of water and dispose of

2O2

Generator with distilled water. Then keep the H2O2 Generator in a clean

2O2

Generator duct. This solution contains H2O2 at a low concentration (about 1% or

WARNING

Never open the door by unlocking it with the unlock key during H2O2 decontamination or H2O2 gas

resolution with the UV lamp. H

gas leakage is potentially harmful to health.

2O2

Precautions when handling H2O2 decontamination reagent

Observe the following precautions when handling the H



Handling Precautions

z

• Wear protective equipment, such as protective glasses and rubber gloves.

• Do not use fire in the area where the reagent is being handled.

• Do not leave any reagent in the container after it has been used or while it is being used.

• Do not place inflammable or combustible materials near the area where the reagent is handled.

Precautions for Storage

z

• Always close the container cover securely to prevent impurities from becoming mixed in the reagent.

• Check the container to make sure that there is no damage, corrosion, or cracking.

• Store the container with the inlet facing upwards, and make sure that the container will not tip or be

knocked over.

• If possible, store the reagent in a location where water is available and where the floor can be easily

washed.

Precautions for Disposal

z

• Dispose according to the rule in your country.

Decontamination Reagent (MCO-H2O2).

2O2



54

ALARMS, SAFETY, AND SELF-DIAGNOSIS

)

The Incubator supports the following alarms, safety functions, and self-diagnostic functions.

Table 1: Alarms and Safety Functions for Culture Operations

Alarm or safety

function

Upper limit
temperature
alarm

Automatic set
temperature
alarm

Automatic set
CO2
concentration
alarm
Automatic set
O2
concentration
alarm

Auto-return

The chamber temperature exceeds the
upper limit alarm temperature set value.

The chamber temperature is out of the
automatic set temperature alarm setting
range (±1.0 to ±5.0

The chamber CO2 concentration is out
of the automatic set CO
alarm setting range (±0.5% to ±5.0%).

The chamber O2 concentration is out of
the automatic set CO
alarm setting range (±0.5% to ±5.0%).

There are no key operations for approx.
90 s in a setting mode.

Key lock The key lock is ON.

Door alarm The outer door is open.

CO2 gas
cylinder empty

The CO2 concentration does not
increase when the CO
opened.

Conditions Display Buzzer Safety operation

Heater OFF
Remote alarm
operates

Remote alarm
operates after delay
of 0 to 15 min.

Remote alarm
operates after delay
of 0 to 15 min.

Remote alarm
operates after delay
of 0 to 30 min.

The setting mode is
canceled.

Setting changes are
prohibited.
The CO
closed.
The N
closed.

valve is

2

2/O2

valve is

The heater turns
OFF after 1 min.

Remote alarm
operates.

o

C).

concentration

2

concentration

2

valve is

2

OVERHEAT indicator lights. Continuous tone

Warning :High Temp or
Warning :Low Temp

is displayed in the message display
field.

Warning :High CO
Warning :Low CO

in the message display field.

Warning :High O
Warning :Low O

in the message display field.

Density or

2

Density is displayed

2

Density or

2

Density is displayed

2

Intermittent tone
after set alarm delay
time (0 to 15 min)
has elapsed
Intermittent tone
after set alarm delay
time (0 to 15 min)
has elapsed
Intermittent tone
after set alarm delay
time (0 to 30 min)
has elapsed

Top screen is displayed. ----­Key Lock is displayed on the standby

operation setting screen.

-----

Intermittent tone

：

Open is displayed in the

Door

message display field.

after set alarm delay
time (1 to 30 min)
has elapsed

Err01: CO

the message display field.

Gas Empty is displayed in

2



Intermittent tone

N2 (or O

cylinder empty

) gas

2

CO2 line
changeover

N2 (or O

changeover

) line

2

Connection
mistake of
CO2 line and
N

(or O

2

line

2

Selection
Mistake of
N2 gas and
O2 gas.

Chamber
temperature
sensor error

Sensor box
temperature
sensor error

Ambient
temperature
sensor error

CO2 sensor
error

The O2 concentration does not increase
when the N

valve is opened.

2/O2

The CO2 gas supply is switched
automatically to the reserve cylinder
(only when the MCO-21GC is installed).

The N2/O2 gas supply is switched
automatically to the reserve cylinder.

The CO

concentration keeps

2

increasing when the CO
closed.

concentration keeps keep

The O

2

changing into the opposite direction
when the N

valve is opened.

2/O2

valve is

2

The chamber temperature sensor is
disconnected.

The chamber temperature sensor is
short-circuited.

The sensor box temperature sensor is
disconnected.

The sensor box temperature sensor is
short-circuited.

The ambient temperature sensor is
disconnected.

The ambient temperature sensor is
short-circuited.

The Vref or Vgas output voltage for the
CO

sensor is abnormal.

2

Err02: N
in the message display field.

Err01: CO

the message display field.
The text flashes for the empty CO
supply line.
Err02: N
in the message display field.
The text flashes for the empty N
gas supply line.

Err03: CO
displayed in the message display field.

Err04: N
displayed in the message display field.

Err05

Gas Empty is displayed

2/O2



Gas Empty is displayed in

2



Gas Empty is displayed

2/O2



Gas Connect Abnormal is

2



Gas Connect Abnormal is

2/O2



：

Temp Sensor Open

2/O2

gas

2

is displayed in the message display
field.

：

Temp Sensor Short

Err06

is displayed in the message display
field.

Err07: CO

is displayed in the message display

S Box Temp Sensor Open

2

field.
Err08：CO2S Box Temp Sensor Short is

displayed in the message display field.
Err09: AT Sensor Open is displayed in

the message display field.
Err10: AT Sensor Short is displayed in

the message display field.

Err11: CO
Err12: CO

displayed in the message display field.

Sensor Vref Abnormal or

2



Sensor Vgas Abnormal is

2



Intermittent tone

〃

〃

〃

〃

〃

〃

〃

〃

〃

〃

〃

Remote alarm
operates.

The CO
is switched.

gas supply

2

Remote alarm
operates.

2/O2

gas

The N
supply is switched.
Remote alarm
operates.
The CO
closed.
The N
closed.
The CO
closed.
The N
closed.

valve is

2

2/O2

valve is

2

2/O2

valve is

valve is

The heater turns
OFF.
Remote alarm
operates.
The heater turns
OFF.
Remote alarm
operates.
CO2 valve is closed.
Remote alarm
operates.

valve is closed.

CO

2

Remote alarm
operates.

Remote alarm
operates.

Remote alarm
operates.

valve is closed.

CO

2

Remote alarm
operates.

55



Alarm or safety

function

O2 sensor error

Main heater
error

Bottom heater
error

Door heater
error

Sensor box
heater error

Heater SSR
burnout
Low humidifying
water

UV lamp failure

New UV lamp
recommended

The measured voltage
for the O

Main heater burnout occurs or the main
heater SSR is short-circuited when the
upper limit temperature alarm operates.

Bottom heater burnout occurs or the
bottom heater SSR is short-circuited.

Door heater burnout occurs or the door
heater SSR is short-circuited.

Sensor box heater burnout occurs
or the sensor box heater SSR is
short-circuited.
Main, bottom, door, or sensor box
heater SSR burnout occurs.
The water in the humidifying pan
drops to approx. 600 ml.
The UV lamp burns out (only for the
MCO-19M(UVH)/19M(UV) or when the
optional MCO-19UVS is installed).
The accumulated ON time reaches
approx. 1,000 hours (only for the
MCO-19M(UVH)/19M(UV) or when the
optional MCO-19UVS is installed).

Conditions Display Buzzer Safety operation

N

2/O2

sensor is abnormal.

2

Err19: O

displayed in the message display field.

Err13: Main Heater Abnormal is
displayed in the message display field.

Err14: Humidity Heater Abnormal

is displayed in the message display
field.

Err15: Door Heater Abnormal is
displayed in the message display field.

Err16: CO

displayed in the message display field.
Err17: Heater SSR Open is displayed

in the message display field.
RH PAN flashes in reverse video in the
status display field.

Err18: UV Lamp Abnormal is displayed
in the message display field.

Warning :UV Life is displayed in the
message display field.

Sensor Abnormal is

2



S Box Heater Abnormal is

2

Intermittent tone

〃

〃

〃

〃

〃

----- -----

Intermittent tone

----- -----

closed.
Remote alarm
operates

Remote alarm
operates.

Remote alarm
operates.

Remote alarm
operates.

Remote alarm
operates.

Remote alarm
operates.

Remote alarm
operates.

∗ The buzzer can be stopped by pressing the BUZZER Key, but remote alarms will not stop. In addition, a

buzzer that sounds because of the upper limit temperature alarm cannot be stopped by pressing the

BUZZER Key.

∗ Err01 recovery is achieved automatically by correctly connecting the gas and pressing the BUZZER Key

to stop the buzzer. In addition, when the MCO-21GC is installed and the gas supply is switched to the

reserve gas cylinder, press the BUZZER Key and replace the gas cylinder.

∗ Err02 recovery is achieved automatically by correctly connecting the gas and pressing the BUZZER Key

to stop the buzzer. In addition, when the gas supply is switched to the reserve gas cylinder, press the

BUZZER Key and replace the gas cylinder.

∗ For Err03, confirm whether a CO2 gas cylinder is correctly connected to a connecting port A/B for CO

gas pipe. Refer to page 16

∗ For Err04, confirm whether a N

N

gas pipe. Refer to page 17

2/O2

(or O2) gas cylinder is correctly connected to a connecting port A/B for

2

∗ If an error from Err05 to Err19 occurs (except for the upper limit temperature alarm in Err13 to Err16),

then consult a Sanyo sales representative or agent.

valve is

2

56



Table 2: Alarm and Safety Functions for H

Alarm and safety

function

System check error
at start of H2O2

decontamination

Failure during H2O2

decontamination

Failure during H2O
gas analysis by UV

The H2O2 Generator is not
connected.

There is no H2O2 solution or
the H
failed (or is disconnected).

A door is not closed.

The volume of H2O2 generated
is abnormal.

Power was interrupted during
H

The UV lamp failed during
H

2

Power was interrupted during
H

Condition Display Buzzer Safety operation

level sensor has

2O2

decontamination.

2O2

gas analysis by UV.

2O2

gas analysis by UV.

2O2

Decontamination

2O2

H2O2 Decon System Error is displayed
in the screen center.
Err31:H2O2 Unit No Connect is
displayed in the message display field.

H2O2 Decon System Error is displayed
in the screen center.
Err32: H2O2 Level is Low is displayed in
the message display field.

H2O2 Decon System Error displayed in
the screen center.
Err33: Door is Open is displayed in the
message display field.

Err34:H2O2 Volume is displayed in the
message display field.

After power is restored, Err35: Power
Failed is displayed in the screen
message display field.
After completion, Decon Failed is
displayed in the screen center.

Err36: UV Lamp Failure is displayed in
the message display field.

After power is restored, Err37: Power
Failed is displayed in the message
display field.

-----

-----

-----

Intermittent
tone with
15min delay

Intermittent
tone

Intermittent
tone

-----

Decontamination mode is
cancelled.

Decontamination mode is
cancelled.

Decontamination mode is
cancelled.

Moves to UV analysis.
Remote alarm operates with 15
min delay.

Electrically locked during power
interruption. After power is
restored, moves to UV analysis.
Remote alarm operates.

UV analysis time is extended.
Remote alarm operates.

Electrically locked during power
interruption. After power is
restored, UV analysis is
repeated.

Table 3: Alarm and Safety Functions for automati c CO2 and O2 density calibration

Alarm and safety

function

System check error
at start/operating of
automatic CO2 and
O2 density
calibration

The gas pressure for the
standard gas calibration CO
gas line is less than the
specified pressure.

Condition Display Buzzer Safety operation

Std Gas Calib Error is displayed in the
screen center.

2

Err41: CO2 Std Gas Empty is displayed
in the message display field.

Intermittent
tone

The standard gas calibration
mode is cancelled.
Remote alarm operates.

Note：

For models with electric locking, an unlock key is provided in order to unlock the door during a power

interruption or in case the electric lock fails. Always store this key in a safe place. It is recommended that

you make a note of the key symbol and number in case the key is lost.

WARNING

Do not use the unlock key to unlock the door during H2O2 decontamination or during H2O2 gas
analysis by UV. Doing so may cause harm to health from H

gas leakage.

2O2

57

 CALIBRATION

Temperature/CO2/O2 calibration

1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER

Key.




1



2




3



4



5



6




7



2. Select Temp/CO2/O2 Calibration from the Select Tools Display, press the MENU Key to display the
menu, select OK, and press the ENTER Key.















3. The Temp/CO2/O2 Calibration Display will appear.



Example

Enter the value shown below if the displayed chamber temperature is 37.0

is 36.5

●Temperature span: Enter 365.




1




3




4



5





6



T e m p 3 7 . 0o C C O 2 5 . 0 %

３７.０

S t a t u s

O K

S e l e c t T o o l s ( 1 / 2 )
T e m p / C O 2 / O 2 C a l i b r a t i o n

A l a r m Se t t i n g
L C D / D A Q S e t t i n g
U V S e t t i n g
K e y L o ck P W S etting
D a t e T i m e

o

C.

T e m p / C O 2 / O 2 C a l i b r a t i o n
P V

T e m p S p a n 3 6 . 5oC37 . 0oC 0 . 5°C
C O 2 Z e r o 3 . 9 1 V 5 0 0
C O 2 S p a n 0 . 0 % 5 . 0 % 3 . 1 6 V 7 0 0
O 2 Z er o 5 0 0
O 2 S p a n 0 . 0 % 5 . 0 % 5 8 m V 5 0 0

５.０

O 2 5 . 0 %

５ .

５.０

Volt

M E N U
S e t
L o g

T o o l s

M E N U

O K

S v c
C a n c e l

o

C but the actual temperature

58



Example

Enter the value shown below if the displayed chamber CO

density is 5.0% but the actual CO2 density is

2

4.5%.

●CO

density span: Enter 045.

2




T e m p / C O 2 / O 2 C a l i b r a t i o n

1




P V

3



T e m p S p a n 0 . 0oC37 . 0oC 0 . 5°C



C O 2 Z e r o 3 . 9 1 V 5 0 0

4



C O 2 S p a n 4 . 5 % 5.0% 3.16 V 7 0 0

5



O 2 Z er o 5 0 0




O 2 S p a n 0 . 0 % 5 . 0 % 5 8 m V 5 0 0

6

Volt



Example

Enter the value shown below if the displayed chamber O

density is 5.0% but the actual O2 density is

2

5.5%.

●O

density span: Enter 055.

2




T e m p / C O 2 / O 2 C a l i b r a t i o n

1




P V

3



T e m p S p a n 0 . 0oC37 . 0oC 0 . 5°C



C O 2 Z e r o 3 . 9 1 V 5 0 0

4



C O 2 S p a n 0 . 0 % 5 . 0 % 3 . 1 6 V 7 0 0

5



O 2 Z er o 5 0 0




O 2 S p a n 5 . 5 % 5 . 0 % 5 8 m V 5 0 0

6

Volt



4. Press the MENU Key to display the menu, select OK, and press the ENTER Key.

5. After the Select Tools Display is displayed, press MENU Key to display the menu, select Cancel, and

press the ENTER Key to return to the Top Display.

Note:

It is important to accurately measure the temperature inside the Incubator when calibrating the

temperature. Particularly, the temperature gauge used must have an accuracy of JIS 0.5 Class or better.

The temperature must be measured at several points.

The temperature setting can not change by more than ±1.0

o

C during calibration. If these values are

exceeded, an error buzzer will sound, the input data will be ignored, and the Incubator will return to the

o

display mode. If it is necessary to change the temperature by more than 1.0

C, perform calibration in

several stages over a period of time.

59

 TROUBLESHOOTING

If the Incubator does not seem to be working properly, check the following items before calling for service.

Symptom Items to check and countermeasures

The Incubator does not
operate at all.

• Is the Incubator plugged in?

• Is there a power outage, or has a circuit breaker interrupted the

power?

The key is not working.

• Is the key lock function turned OFF?

An alarm has occurred. When starting operation:

• Does the chamber temperature match the set value?

• Does the CO

gas concentration in the chamber match the set

2/O2

value?

(1) Is the secondary pressure for the CO

specified value of 0.03 MPaG (0.3 kgf/cm

(2) Is the secondary pressure for the N

specified value of 0.05 MPaG (0.5 kgf/cm

(3) Is the gas tube properly connected?

During operation:

• Is the upper limit alarm temperature set at least 1°C higher than the
chamber set temperature?

• Has the temperature setting been changed? Has the door been left
open for a long time? Has a low-temperature object been placed in the
chamber? If any of these is the case, the alarm will be automatically
cleared if you wait.

• Has the gas tube come loose, or is there a gas leak?

gas pressure regulator at the

2

2

G. 4.3 psiG)?

gas pressure regulator at the

2/O2

2

G, 7.1 psiG)?

The chamber temperature
does not match the set value.

The gas concentration does
not match the set value.

The chamber temperature
does not rise.

• Has the CO

/O2 gas concentration setting been changed?

2

• Is the gas cylinder empty? Check the primary pressure of the gas
cylinder once a week. (When the primary pressure is 3.8 MPa (38
kgf/cm

2

G) or lower, it is a sign that there is little gas remaining.

Replace the cylinder soon.)

• Is the ambient temperature less than 5°C different from the set value
for the chamber temperature?

• Is the outer door closed with the gastight split doors or inner door left
open?

• Is the secondary pressure for the CO
specified value of 0.03 MPaG (0.3 kgf/cm

• Is the secondary pressure for the N
specified value of 0.05 MPaG (0.5 kgf/cm

gas pressure regulator at the

2

2

G, 4.3 psiG)?

gas pressure regulator at the

2/O2

2

G, 7.1 psiG)?

• Is the gas tube blocked?

• Is there enough water in the humidifying pan? (Be sure to use sterile

distilled water.)

60



Symptom Items to check and countermeasures

A large quantity of gas is being
consumed.

• Are the doors being frequently opened and closed?

• Check whether gas is leaking from connectors due to deterioration

of the gas tube, or whether there may be any pinhole leaks. The
gas tube is a replaceable part, and it is recommended that it be

replaced once a year.

• Is the packing seal for gastight split doors and inner door
defective?

• Is the access hole open?

Normal cultures are not possible,
and the gas concentration is
suspect.

The gas concentration is slow to
recover.

• Is the ambient air environment around the Incubator normal? Is
there a source of polluted gas in the vicinity?

• A HEPA filter is used for the Incubator gas piping. If gas
concentration is slow to recover when the CO

gas concentration is

2

normal, it is possible that the HEPA filter may be clogged. Consult a
Sanyo representative or agent.

• Is there little gas remaining in the gas cylinder?

• Is the secondary pressure for the CO

specified value of 0.03 MPaG (0.3 kgf/cm

• Is the secondary pressure for the N
the specified value of 0.05 MPaG (0.5 kgf/cm

gas pressure regulator at the

2

2

G, 4.3 psiG)?

/CO2 gas pressure regulator at

2

2

G, 7.1 psiG)?

• Is the gas tube blocked?

The gas is not being injected.

• The control method for the Incubator is the ON-OFF method. Gas
is intermittently injected as the gas concentration in the chamber
approaches the set value. Injections may be stopped for periods of
approximately 60 seconds, but that is not an error.

The outer door does not open. MCO-19M(UVH), or when the optional MCO-HL is installed:

• When the power is OFF, the electric lock is locked and the outer
door does not open. Either turn ON the power or use the accessory
unlock key to override the electric lock.

• During decontamination the outer door is electrically locked and
does not open.

H2O2 decontamination cannot be
performed.

• If the MCO-19M(UV)/19M is being used, is the optional MCO-HL
installed?

• If the MCO-19M is being used, are the optional MCO-HL and
MCO-19UVS installed?

• Is the UV lamp burned out? If the UV lamp is burned out, H
decontamination will not be possible.

• Is the H

• Has the entire bottle of H

• Has

the upper limit temperature alarm been set to 50°C or higher?

Generator cable properly connected?

2O2

Decontamination Reagent been used?

2O2

Note: If the problem still has not been solved after trying the above checks and countermeasures, or for

any problems not covered here, contact a Sanyo representative or agent.

61

2O2

DISPOSING OF THE MULTI-GAS INCUBATOR

When disposing of the multi-gas incubator, contact a Sanyo sales representative or agent.

WARNING

The incubator must be dismantled and disposed of by qualified personnel only. If the incubator is

left where outsiders enter, it may result unexpected accident (for example, children to become locked

inside).

Before disposing a multi-gas incubator with biohazardous danger, decontaminate the multi-gas incubator

to the extent possible by the user.

62

Note:
This symbol mark and recycle system apply only to EU countries. They
do not applied to the countries in other areas of the world.

Waste Electrical and Electronic Equipment (WEEE) Directive 2002/96/EC

(English)

This SANYO product is designed and manufactured with high-quality materials and components that can
be recycled and reused.

This symbol means that the electrical and electronic equipment, at their end-of-life, should be disposed of
separately from household wastes.

Dispose of this product at your local community waste collection/recycling center.

In the European Union there are separate collection systems for used electrical and electronic products.

Please help us to conserve the environment we live in.

(German)

Ihr SANYO Produkt wurde entworfen und hergestellt mit qualitativ hochwertigen Materialien und
Komponenten, die recycelt und wiederverwendet werden können.

Dieses Symbol bedeutet, daß elektrische und elektronische Geräte am Ende ihrer Nutzungsdauer von
Hausmüll getrennt entsorgt werden sollen.

Bitte entsorgen Sie dieses Gerät bei Ihrer örtlichen kommunalen Sammelstelle oder im Recycling Centre.

In der Europäischen Union gibt es unterschiedliche Sammelsysteme für Elektrik- und Elektronikgeräte.

Helfen Sie uns bitte, die Umwelt zu erhalten, in der wir leben!

63

(French)

Votre produit Sanyo est conçu et fabriqué avec des matèriels et des composants de qualité supérieure qui
peuvent être recyclés et réutilisés.

Ce symbole signifie que les équipements électriques et électroniques en fin de vie doivent être éliminés
séparément des ordures ménagères.

Nous vous prions donc de confier cet équipement à votre centre local de collecte/recyclage.
Dans l’Union Européenne, il existe des systèmes sélectifs de collecte pour les produits électriques et
électroniques usagés.

Aidez-nous à conserver l’environnement dans lequel nous vivons !

Les machines ou appareils électriques et électroniques contiennent fréquemment des matières qui, si elles
sont traitées ou éliminées de manière inappropriée, peuvent s’avérer potentiellement dangereuses pour la
santé humaine et pour l’environnement.
Cependant, ces matières sont nécessaires au bon fonctionnement de votre appareil ou de votre machine.
Pour cette raison, il vous est demandé de ne pas vous débarrasser de votre appareil ou machine usagé
avec vos ordures ménagères.

(Spanish)

Los productos SANYO están diseñados y fabricados con materiales y componentes de alta calidad, que
pueden ser reciclados y reutilizados.

Este símbolo significa que el equipo eléctrico y electrónico, al final de su ciclo de vida, no se debe
desechar con el resto de residuos domésticos.

Por favor, deposite su viejo “televisor” en el punto de recogida de residuos o contacte con su
administración local.

En la Unión Europea existen sistemas de recogida específicos para residuos de aparatos eléctricos y
electrónicos.

Por favor, ayúdenos a conservar el medio ambiente!

64

(Portuguese)

O seu produto SANYO foi concebido e produzido com materiais e componentes de alta qualidade que
podem ser reciclados e reutilizados.

Este símbolo significa que o equipamento eléctrico e electrónico no final da sua vida útil deverá ser
descartado separadamente do seu lixo doméstico.

Por favor, entregue este equipamento no seu ponto local de recolha/reciclagem.

Na União Europeia existem sistemas de recolha separados para produtos eléctricos e electrónicos
usados.

Por favor, ajude-nos a conservar o ambiente em que vivemos!

(Italian)

Il vostro prodotto SANYO è stato costruito da materiali e componenti di alta qualità, che sono riutilizzabili o
riciclabili.

Prodotti elettrici ed elettronici portando questo simbolo alla fine dell’uso devono essere smaltiti
separatamente dai rifiuti casalinghi.

Vi preghiamo di smaltire questo apparecchio al deposito comunale.
Nell’Unione Europea esistono sistemi di raccolta differenziata per prodotti elettrici ed elettronici.

Aiutateci a conservare l’ambiente in cui viviamo!

65

(Dutch)

Sanyo producten zijn ontwikkeld en gefabriceerd uit eerste kwaliteit materialen, de onderdelen kunnen
worden gerecycled en weer worden gebruikt.

Het symbool betekent dat de elektrische en elektronische onderdelen wanneer deze vernietigd gaan
worden , dit separaat gebeurt van het normale huisafval.

Zorg ervoor dat het verwijderen van de apparatuur bij de lokaal erkende instanties gaat gebeuren.
In de Europese Unie wordt de gebruikte elektrische en elektronische apparatuur bij de daarvoor wettelijke
instanties aangeboden.

Alstublieft help allen mee om het milieu te beschermen.

(Swedish)

Din SANYO produkt är designad och tillverkad av material och komponenter med hög kvalitet som kan
återvinnas och återanvändas.

Denna symbol betyder att elektriska och elektroniska produkter, efter slutanvändande, skall sorteras och
lämnas separat från Ditt hushållsavfall.

Vänligen, lämna denna produkt hos Din lokala mottagningstation för avfall/återvinningsstation.

Inom den Europeiska Unionen finns det separata återvinningssystem för begagnade elektriska och
elektroniska produkter.

Vänligen, hjälp oss att bevara miljön vi lever i!

66

AUTOMATIC CO2 CYLINDER CHANGEOVER

An automatic CO

system switches the gas supply lines when one CO

Note: The installation of the MCO-21GC must be performed only by qualified service personnel.

Install the MCO-21GC and then perform the following procedure



1. Connect 2 gas tubes to connecting ports A/B for CO
located on the left back of the Incubator. (Refer to page 9).
Note: Only port A can be used when the MCO-21GC is not installed. There will be 2 ports, ports A and B,
after the MCO-21GC is installed.



2. Connect a CO

for information on connecting the gas cylinders. (“Gas cylinder A” is the CO2 gas cylinder connected to

the connecting port A for CO

connecting port B for N



3. Open the valves of both gas cylinders.



4. The current CO

(“A” and “B” will be displayed only when the MCO-21GC is installed. Nothing will be displayed if

MCO-21GC is not installed.)







1




2



3



4



5




6



7



5. The MCO-21GC detects that there is no more CO

chamber does not increase for a while even after opening the CO

the gas supply line when it detects that there is no more gas. At that time, the following indications of

switching the gas cylinder are provided.



When CO2 gas supply line switches

automatically (Only when MCO-21GC

is installed)

●To silence the buzzer, press the BUZZER Key. The alarm condition is cleared and normal operation

continues.

T e m p 3 7 . 0o C C O 2 5 . 0 % A B O 2 5 . 0 % A B

３７.０

S t a t u s

O K

cylinder changeover system (MCO-21GC) is available as an optional accessory. This

2

gas cylinder becomes empty.

2

gas pipe. Connecting ports for CO2 gas pipes are

2

gas cylinder equipped with a gas pressure regulator to each gas tube. Refer to page 16

2

gas pipe, and “gas cylinder B” is the CO2 gas cylinder connected to the

2

gas pipe.)

2/O2

gas supply line is displayed on the Top Display.

2

Current CO2 gas supply line

５.０

gas in a cylinder when the CO2 density in the

2

2



Message display field Display of gas supply

Err01: CO

displayed.

Gas Empty is

2



５ .

５.０

gas valve in the Incubator. It switches

The character for gas supply line

that is empty blinks.

The gas supply line that is

currently being used is displayed

in reverse video.

67

6. When gas cylinder A is switched to B, remove gas cylinder A and replace it with new one.
Note: Be careful when handling the empty CO

●When gas cylinder B becomes empty, the line is switched to gas cylinder A again.

The MCO-21GC detects that there is no more CO

does not increase for a while even after opening the CO

also be switched for other reasons even though there is gas left in the cylinder: blocking or restricting of

gas tube, reduction of CO

quantity in the cylinder before disconnecting it.



● Use the following procedure to manual switch the line between CO

MCO-21GC is installed). This procedure shows switching the line fromCO
example.



1. From the Top Display, press the Left Cursor Key for 3 seconds.





1



2



3



4




5



6



7




2. The CO




1



2




3



4



5



6




7


(When the line needs to be switched from cylinder A to B, press the Right Cursor Key for 3 seconds.) 



T e m p 3 7 . 0o C C O 2 5 . 0 % A B O 2 5 . 0 % A B

３７.０

S t a t u s

O K

gas supply line will be switched from cylinder B to A

2

T e m p 3 7 . 0o C C O 2 5 . 0 % A B O 2 5 . 0 % A B

３７.０

S t a t u s

O K

gas pressure, or improper opening of CO2 gas cylinder. Always check the gas

2

５.０

５.０

gas cylinder. Some gas may still be left in the cylinder.

2

gas in a cylinder when the CO2 density in the chamber

2

gas valve in the Incubator. The supply line may

2

cylinders A and B (only when

2

cylinder B to A as an

2

５ .

５.０

５ .

５.０

68

AUTOMATIC CO2 AND O2 DENSITY CALIBRATION



To calibrate the CO

By connecting a standard gas cylinder that has a density that is the same as the set CO

(usually 5% CO

the CO



● Setting connected standard gas density

1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER

Key.













2. Press the Down Cursor Key from the Select Tools (1/2) Display to display the Select Tools (2/2) Display,
and then select Standard Gas Setting, press the MENU Key to display the menu, select OK, and press

the ENTER Key.






























and O2 density display as necessary.

2

1

2

3

4

5

6

7

T e m p 3 7 . 0o C C O 2 5 . 0 %

３７.０

S t a t u s

O K

S e l e c t T o o l s ( 1 / 2 )
T e m p / C O2 / O 2 C alibration

A l a r m Se t t i n g
L C D / D A Q S e t t i n g
U V S e t t i n g
K e y L o ck P W S etting
D a t e T i m e

S e l e c t T o o l s ( 2 / 2 )
S t a n d a r d G a s S e t t i n g

S t a n d a r d G a s C a l i b r a t i o n

and O2 density, a Semi-automatic one point Gas Calibration Kit (MCO-SG) is available.

2

and O2 density

2

and 5% O2), the CO2 sensor and O2 sensor can be calibrated to maintain the accuracy of

2

５.０

O 2 5 . 0 %

５ .

５.０

M E N U
S e t
L o g

T o o l s

M E N U

O K

S v c
C a n c e l

M E N U

O K

S v c
C a n c e l

69



3. Input the CO

select OK, and press the ENTER Key.




S t a n d a r d G a s S e t t i n g

1




3



S t d G a s C O 2 D e n s i t y 5 . 0 % ( 4 . 0 % - 2 1 . 0 % )



( N o Calibrat i o n : 0 . 0 % )

4



S t d G a s O 2 D e n s i t y 5 . 0 % ( 0 . 5 % - 1 8 . 0 % )

5



( 2 2 . 0 % - 8 1 . 0 % )



( N o Calibration:18.1 % - 2 1 . 9%)



6



Ex. Standard gas density input

Standard gas density

Calibrate both of CO2 and O2 density CO2:5% / O2:5% 050 050

Calibrate only CO2 density CO2:5% / O2:20% 050 200

Calibrate only O2 density CO2:0% / O2:5% 000 050

● Calibration method for CO

1. Select Standard Gas Calibration from the Select Tools (2/2) Display, press the MENU Key to display
the menu, select OK, and press the ENTER Key.




S e l e c t T o o l s ( 2 / 2 )



S t a n d a r d G a s S e t t i n g



S t a n d a r d G a s C a l i b r a t i o n









2. The system check will start.




S t a n d a r d G a s C a l i b r a t i o n S t e p ： 1

1



3



S t d G a s C a l i b S y s t e m C h e c k ・・・・・




4



CO2： 5. 5% O 2 ： 4 . 5 %

5



STG： 5. 0% STG ： 5 . 0 %




6




and O2 density of standard gas to be used, press the MENU Key to display the menu,

2

M E N U

O K

C a n c e l

and O2 gas density

2

(For example)

Input value of

CO

density

2

Input value of

O2 density

M E N U

O K

S v c
C a n c e l

70



3. If the system is normal, the following display will appear. Select OK and press the ENTER Key to start

calibrating the CO














































S t a n d a r d G a s C a l i b r a t i o n S t e p ： 2

1

3

S t d G a s C alib Start?

4

CO2： 5. 5% O 2 ： 4 . 5 %

5

STG： 5. 0% ST G ： 5 . 0 %

6

S t a n d a r d G a s C a l i b r a t i o n S t e p ： 3

1

3

S t d G a s C a l i b r a t i n g ・・・・・

4

CO2： 5. 5% O 2 ： 4 . 5 %

5

STG： 5. 0% ST G ： 5 . 0 %

6

1

2

3

4

5

6

7

T e m p 3 7 . 0 o C C O 2 5 . 0 % A B O 2 5 . 0 % A B

３７.０

S t a t u s

O K

and O2 density. After calibration, return to normal cultivating operation.

2

M E N U

O K
C a n c e l

５.０

５ .

５.０

71

STACKING INCUBATORS

Use the following procedure to stack Incubators. This work is potentially dangerous, so contact a Sanyo

representative or agent.

CAUTION

• Select a floor that is strong enough to support the stacked incubators.

• Never stack three or more incubators. Doing so is dangerous.

1. Take out two stacking plates A, two stacking plates B, four protective stickers, and four screws from

accessory bag.

Note:

When stacking two incubators, use two stacking plates (A and B) for each one. Only one pair of

stacking plates is provided per incubator, so take out the stacking plates (A and B) from both incubators.

Similarly only 2 protective stickers are provided per incubator

2. Unplug both of the Incubators that are to be stacked.

3. Remove the caps at the two places in the front of the top surface of the Incubator that is to be on the

bottom, attach the protective stickers to the four corners on the top surface of the Incubator that is to be

on the bottom. (See Fig. A.)

4. Secure stacking plate A with the screws that are provided.

5. Remove the front panel (5 screws) from the Incubator that is to be on the top.

6. Place the upper Incubator on top of the lower one, being careful not to pinch your fingers.

7. Make the lower Incubator level by adjusting the legs, and then level the upper Incubator in the same

way.

8. Secure the stacking plate A fixed to the lower incubator to the upper Incubator with the accessory

screws.

9. Remove from the back of the lower Incubator the two hooks (4 screws) and remove from the upper

Incubator the 2 screws on the lower rear panel.

10. Use the 6 screws that were removed in step 9 to secure stacking plate B to the rear panels of the

upper and lower Incubators.

11. Replace the front panel (5 screws) to the upper Incubator.

12. Use the hooks on the back of the upper Incubator to prevent the Incubators from falling over.

72

Front panel

Front

Stacking plate A

Stacking plate B

Protective sticker

Hook

Fig.A

73

SPECIFICATIONS

Name Multi-gas Incubator

Models MCO-19M MCO-19M(UV) MCO-19M(UVH)

External dimensions W620 x D710 x H900 mm (W24.4 x D28.0 x H35.4 inch)

Internal dimensions W490 x D523 x H665 mm (W19.3 x D20.6 x H26.2 inch)

Interior volume 162 L (5.72 cu.ft.)

Exterior Painted steel

Interior Stainless steel containing copper

Outer door Painted steel
Inner door Stainless steel with gasket

Gastight split door 4 doors made of tempered glass

Trays 3 trays made of stainless steel containing copper

W450 x D450 x H12 mm (W17.7 x D17.7 x H0.47 inch), Maximum load: 7 kg/tray
Access port Inner diameter: 30 mm (1.18 inch), On the back side

Insulation Rigid polyurethane foamed-in place

Heating system DHA system (heater jacket + air jacket system)

Heater 314 W

Humidifying system Natural evaporation with humidifying pan

Temperature controller PID control system

Temperature display Digital display

CO2 controller PID control system

CO2 density display Digital display

O2 controller PID control system

O2 density display Digital display

Air circulation Fan assisted

Air filter 0.3 µm, Efficiency: 99.97% or higher

UV lamp

Water level sensor

Alarms

Remote alarm contacts Allowable contact capacity: 30 VDC, 2 A

CO2 inlet connection 4 to 6 mm (0.157 to 0.236 inch) diameter tube can be connected

CO2 inlet pressure 0.03 MPaG (0.3 kgf/cm2G, 4.3 psiG)

O2 inlet connection 4 to 6 mm diameter tube can be connected

O2 inlet pressure 0.05 MPaG (0.5 kgf/cm2G, 7.1 psiG)

Weight 94 kg

Automatic set temperature alarm, Automatic set CO2 density alarm

Automatic set O2 density alarm, Upper limit temperature alarm

----- 4W X 1 (ozone-free emission)

Optical

various gas/sensor/heater alarms

74

Name Multi-gas Incubator

Models MCO-19M MCO-19M(UV) MCO-19M(UVH)

3 trays, 3 sets of tray supports, 3 gas tubes, 1 humidifying pan,

Accessories

1 pair of stacking plates A and B, 2 protective stickers, 6 tube bands

H

2O2

UV Lamp Add-on Kit

(MCO-19UVS)

Optional accessories

Half Trays (MCO-25ST), Automatic CO

Roller Base (MCO-18RB), UV lamp replacement kit (MCO-20UV)

Stacking spacer (MCO-18SB, when stacking on the MCO-175),

Stacking spacer (MCO-21SB, when stacking on the MCO-20AIC)

Ethernet (LAN) Interface (MTR-L03), RS232C/RS485 Interface (MTR-480)

Note: Designs and specifications are subject to change without notice.

1 gas injection nozzle, 1 gas injection nozzle tube

H

Generator (MCO-HP)

2O2

Decontamination Kit (MCO-HL)

Standard equipment

H2O2 Decontamination Reagent (MCO-H2O2),

Automatic CO

Standard Gas Calibration Kit (MCO-SG),

2

Gas Pressure Regulator (MCO-100L),

Extra Trays (MCO-47ST, includes 2 tray supports),

Cylinder Changeover System (MCO-21GC),

2

SANYO Data acquisition software (MTR-5000)

4-20mA Interface (MCO-420MA, USA only)

Standard equipment

PERFORMANCE

Temperature control range

Temperature distribution

Temperature variation

CO2 control range

CO2 variation

O2 control range

O2 variation

Chamber humidity

Applicable environment
condition

Noise level

Power consumption

Heat emission Max. 1,274 kJ/h
Rated voltage, frequency
Amperage

Note: The unit with CE mark complies with EC directives.

Based on our measuring method.

Designs and specifications are subject to change without notice.

Ambient temperature+5 to 50

o

± 0.25

o

± 0.1

C (ambient temperature: 25oC, setting: 37oC, CO2: 5%, O2: 5%, no load)

± 0.15% (ambient temperature: 25

± 0.2% (ambient temperature: 25

Max. 354 W Max. 335 W

110 to 120 V, 60 Hz 220 V, 60 Hz 220 to 240 V, 50 Hz

Max. 3.1 A Max. 1.5 A Max. 1.5 A

o

C (ambient temperature: 5 to 35oC)

C (ambient temperature: 25oC, setting: 37oC, CO2: 5%, O2: 5%, no load)

0 to 20%

o

C, setting: 37oC, CO2: 5%, O2: 5%, no load)

1 to 18% and 22 to 80%

o

C, setting: 37oC, CO2: 5%, O2: 5%, no load)

95% ± 5%

Temperature: 5 to 35

o

C, Humidity: 80% max.

(The designed performance may not be obtained

If the ambient temperature is equal or less than 15

o

C)

30 dB (A scale)

Max. 1,206 kJ/h

75

CAUTION

Please fill in this form before servicing.

Hand over this form to the service engineer to keep for his and your safety.

Safety check sheet

1. Unit contents
Risk of infection:
Risk of toxicity:
Risk from radioactive sources:

(List all potentially hazardous materials that have been stored in this unit.)
Notes :

2.

Contamination of the unit

Unit interior
No contamination
Decontaminated
Contaminated
Others:

3. Instructions for safe repair/maintenance of the unit
a) The unit is safe to work on
b) There is some danger (see below)
Procedure to be adhered to in order to reduce safety risk indicated in b) below.

Date :
Signature :

Address, Division :

Telephone :

□Yes □No

□Yes □No
□Yes □No

□Yes □No
□Yes □No

□Yes □No

□Yes □No
□Yes □No
□Yes □No

□Yes □No
□Yes □No

Product name :

Multi-gas incubator

Please decontaminate the unit yourself before calling the service engineer.

Model :

MCO-19M(UVH)

MCO-19M(UV)

MCO-19M

Serial number : Date of Installation :

76

7FB6P101569000

(

26 August 2010)

5-5, Keihan-Hondori 2-Chome
Moriguchi City, Osaka 570-8677 Japan

Printed in Japan