Sanyo MCO-19M, MCO-19AIC UV, MCO-19M UVH, MCO-19AIC, MCO-19M UV User Manual

Sterisonic™ GxP
Series Cell Culture
Incubators

CO2 Incubators

MCO-19AIC(UVH)
MCO-19AIC(UV)
MCO-19AIC

CO

/ O2 Incubators

2

MCO-19M(UVH)
MCO-19M(UV)
MCO-19M

Features:

• The industry’s most complete cell culture
solution for highly regulated applications or
conventional incubation.

• Safe, effective and documented two-hour in situ

decontamination for the fastest turn-around.

H

2O2

www.sanyobiomedical.com

My Life. My Work. My Choice.

MCO-19AIC

MCO-19M
MCO-19AIC(UV)
MCO-19AIC(UVH)

MCO-19M(UV)

MCO-19M(UVH)

Sterisonic™GxP Series Cell Culture CO2 and CO2 / O2 Incubators

The industry’s most complete cell culture solution for highly regulated applications or conventional
incubation. Now with safe, effective and documented two-hour in situ H
est turn-around and maximum availability.

Sterisonic™ GxP Selection

Feature

Vapor Decontamination Standard Optional Optional Standard Optional Optional

H

2O2

™

SafeCell

UV Decontamination Standard Standard Optional Standard Standard Optional

Single Beam, Dual Detector IR CO

Oxygen Concentration Control, Zirconia Control N/A N/A N/A Standard Standard Standard

Sensor Standard Standard Standard Standard Standard Standard

2

MCO-

19AIC(UVH)

CO2 Incubators CO2/O2 Incubators

MCO-

19AIC(UV)

MCO­19AIC

decontamination for the fast-

2O2

MCO-

19M(UVH)

MCO-

19M(UV)

MCO-

19M

Product Applications

The combination of Sterisonic™ GxP
incubator performance functions permit
use with condence in high-value cell
protocols among hard-to-grow cell lines,
cells highly sensitive to contamination,
ultra-sensitive media and reagents, or
protocols that require a strict isolation
and decontamination between process­es. These include but are not limited to:

• Stem cell research

• Autologous tissue regeneration

and regenerative medicine

• In vitro fertilization

1

• Genomic and proteomic expression

• Esoteric plant and amphibian

cell culture

• Hypersensitive and transgenic

cell culture

• Low media volume microplate work

1

501(k) clearance applied for. Contact SANYO for status. MCO-19AIC
and MCO-20AIC CO2 incubators have received U.S. Food and Drug
Administration 510(k) clearance for in vitro fertilization applications in
accordance with the FDA Safe Medical Devices Act of 1990 and the Medical
Device Amendments of 1992. Reference: Number K013703. Regulation
Number: 21 CFR 884-6120, Assisted Reproduction Accessories, Regulatory
Class II, Product Code 85MOG, October 30, 2001

Designed for use with a variety
of standard cell culture vessels
and protocols.

• Interior components and adjustable
shelves are congured for easy access,
in situ decontamination and exible ar­rangement for a variety of applications.

• Four adjustable shelves are included,
standard; maximum shelf capacity
is 15 shelves.

MC O -19AIC (UV H)

MC O -19 M

Multi-Gas Incubator, Oxygen
and CO2 Control

• Ideal for in vitro fertilization (IVF), genetic
research, regenerative medicine and
other protocols that require CO
sub-ambient (hypoxic) or above-ambient
oxygen control.

• Cabinet based on MCO-19 platform
with inCu safe
stainless steel chamber, continuous
contamination control, patented D.H.A.
multi-point, air-jacketed temperature
control system, elevated relative humidity
with integral water-level sensor.

™

copper-enriched

and

2

2 Sterisonic™ GxP Series Cell Culture Incubators

Sterisonc™ GxP Features and Benefits

The SANYO Sterisonic™ GxP is designed for a wide array of demanding and highly regulated applications in the biomedical, pharmaceutical,
medical research and clinical laboratory. Representing years of research, development and component testing, the Sterisonic™ GxP incorporates a
collective of mutually functional systems and design attributes to offer a holistic solution to cell culture protocols, from the most sophisticated to
more familiar and conventional processes.

Decontamination System

• The unique Sterisonic™ GxP H2O2
decontamination system limits down­time to less than three hours when
total chamber decontamination with
verication is desired.

• All interior components and CO
sampling loop are sterilized in situ;
no need for removal and autoclaving.

• Active Background Contamination
Control

™

ghts contamination while

cell culture protocols are in process.

The patented SafeCell

system scrubs interior airow
to destroy airborne and humidity pan
contaminants.

Exclusive inCu saFe

™

enriched stainless steel interior
surfaces assure constant germicidal
protection.

Shelf brackets are formed
with an exaggerated angle
to minimize surface contact
with flat shelves*.

™

UV

copper-

2

Control and Monitoring

• The Sterisonic™ GxP control and
information center includes an intuitive
pop-up menu, high resolution LCD
for inputs, outputs and performance
at-a-glance.

• Multi-point data logging offers
push-button graphical display. An
optional PC interface permits remote
transmission for GMP/GLP protocols
as required.

• Precise PID logic controls and
adjusts to all temperature, CO

set-

2

points and alarm parameters.

CO2 Control

SANYO proprietary single-beam,
dual detector infrared (IR2) CO

sensor delivers precise CO

control,

2

quick recovery following door openings,
and auto sampling with no moving parts.

• Continuous zero calibration is standard.

• An optional semi-automatic, one-point
calibration system is available. Catalog
No. MCO-SG; see Accessories.

2

Zirconia O2 Control System,
MCO-19M Series

A zirconia oxygen sensor main-

tains sub-ambient O
1% to 18%. Additionally, enriched O
levels from 22% to 80% are enabled
with proper safety precautions.

• Concurrently the MCO-19M permits

a CO

range 0% to 20% via infrared

2

sensor.

• Nitrogen gas bubbler accelerates

recovery of chamber humidity levels
following door openings.

• An electronic P.I.D. control maintains

accurate temperature and gas set­points over the entire system range.

• The MCO-19M includes an automatic

gas switchover system that changes
from the primary to a secondary gas
cylinder for either oxygen or nitrogen;
an optional second gas switchover
system is available for CO

• Cabinet based on MCO-19 platform

with inCu saFe

™

copper-enriched
stainless steel chamber, continuous
contamination control, patented D.H.A.
multi-point, air-jacketed temperature
control system, elevated relative hum­idity with integral water-level sensor.

levels from

2

.

2

2

MC O-19 M

MCO-19M shown with
four separate inner
doors with gaskets.

3

Sterisonc™ GxP Features and Benefits

Temperature
and Humidity Control

• The patented Direct Heat and Air™
conditioning system manages setpoint
temperature through multiple, variable
warming points under microprocessor
control.

• The humidity pan is easy to ll, easy
to clean; the automatic optical sensor
advises of low water level.

H2O2 Decontamination

The use of H2O2 decontamination
in biological safety cabinets and barrier
isolators is a popular alternative to ethyl­ene oxide (EtO) as a safer, more efcient
decontamination method. H
been widely used in the pharmaceutical
industry. In aerospace research, H
used to sterilize satellites.

The FDA has recently granted 510(k)
clearance to use H

in individual medi-

2O2

cal device manufacturing applications.
EtO criteria outlined in ANSI/AAMI/ISO

has long

2O2

2O2

is

14937 may be used as a validation guide­line. For references online visit
www.sanyobiomedical.com/sterisonic.

Unlike conventional incubators, unique
features of the SANYO Sterisonic
GxP incubator permit use of the H
process in situ with complete safety,
zero impact on adjacent equipment or
the environment, and speed to return
the incubator to service.

• The H
functions with the patented SANYO
SafeCell

decontamination process

2O2

™

UV system. Following a
seven-minute H
circulation and dwell cycle, vaporization
is stopped and the SafeCell
turned ON for up to ninety minutes.

• When exposed to UV light, the H
vapor breaks down into water and
oxygen, leaving only traces of water
droplets. These droplets automati­cally condense onto a naturally cooler
section of the interior oor for easy
wipe-up.

• Throughout the entire cycle the
Sterisonic

™

GxP airow system con­tinues to gently circulate interior air
assuring 100% vapor contact with all
interior surfaces, ultimately creating
a serial dilution of H
over the UV lamp.

vaporization,

2O2

as it passes

2O2

™

2O2

™

UV lamp

2O2

• Orientation of interior sample ports
of the single beam, dual detector IR
CO

sensor creates a slight Venturi

2

ow through the sample chamber, per­mitting total decontamination of the
CO

system at the same time.

2

• Shape and location of interior compo­nents such as shelves, shelf brackets,
plenum covers and the humidity pan
permit the components to remain
in the chamber during the decontami­nation process, conveniently bypass­ing the need for a separate autoclave
cycle.

• Once the cycle is complete, the door
locking system is released; the inner
door can be opened, interior compo­nents repositioned and the incubator
is returned to service.

Ergonomic Cabinet Design

With reversible inner and outer doors,
a single SANYO incubator offers the
industry's most exible installation
options available.

• Low prole cabinet with door-mounted
control panel permits easy access and
viewing.

• The outer door latch and door heater
cable is easily switched if a reverse
opening is required.

Sterisonic™ GxP Series H2O2 Decontamination Performance Value

2 Hours

SANYO
Sterisonic™

H2O

decontamination vs. high heat decontamination

2

= Uptime (Hours)

The documented two-hour in situ H2O2 sequence puts the fully sterilized Sterisonic™ GxP available and
ready for use quicker than any other incubator worldwide.

4 Sterisonic™ GxP Series Cell Culture Incubators www.sanyobiomedical.com

Brand X

=

Downtime (Hours)

14 Hours

Sterisonc™ GxP Features and Benefits

• Cabinet knock-outs are pre-drilled
and tapped to eliminate drilling and to
simplify re-mounting of door hardware.

• The outer door closes against a
soft, easily cleaned magnetic gasket
designed to eliminate ambient air
shear across the glass inner door,
minimizing condensation.

• A door ajar alarm provides an audible
and visual warning if the outer door
is left open.

• A center-mounted key lock is located
beneath the door for added security;
model MCO-19AIC(UVH) only.

Shelves and Inventory
Management

Convenient space efcient inventory
management is simplied through a sys­tem of adjustable, extendable shelves.

• Inventory shelves and brackets are
formed from polished copper-enriched
stainless steel, removable without
tools, and can remain inside the
incubator during the H
nation cycle or autoclaved separately if
desired.

decontami-

2O2

• Shelves are perforated to permit natu­ral vertical air convection through and
around labware.

• Shelf brackets are formed with an
exaggerated angle to minimize surface
contact with at shelves (patent pend­ing). Brackets slip easily into vertical
supports that attach to interior cham­ber walls with clearance sufcient to
permit proper air circulation.

Inner Door and Gasket

The inner design is critical to successful
contamination control technique.

• The inner gasket body forms an
effective thermal transition between
the ambient air and warm, humidied
incubator atmosphere, minimizing
condensation and eliminating moisture
traps which can harbor contaminants.

• The inner door gasket is a dual
durometer extrusion (two levels of
softness) from closed-cell silicone
to inhibit contamination.

• The gasket feather-edge allows
the inner glass door to close gently
against the chamber opening for
a tight peripheral seal.

• The entire inner door gasket is remov­able for cleaning and/or replacement
if required.

• Radiant heat from the outer door,
apportioned by the microprocessor

MCO-38AIC(UVH)

Stacking Doubles Capacity
in Same Footprint.

• The Sterisonic™ GxP cabinet is
designed for stacking, allowing one
unit to be positioned on top of
another, doubling interior volume
without additional oor space.

• The combination of stacking and
reversible doors offers the most
installation options possible.

• An optional roller base is recom­mended for stacked installations
to permit mobility if required;
see Accessories.

control as a function of the patented
Direct Heat and Air Jacket

™

, automati­cally warms the inner door glass
in proportion to total heat demand
and condensation control.

Field Reversible Door

The eld-reversible door allows
universal installation using the left-hand
hinge (standard) or a right-hand hinge
modication

• The outer door includes a universal
nger grip at each side.

• Mounting holes for hinge hardware
are pre-drilled and capped with easily
removable trim plugs.

• The door heater cable plugs into the
alternate connection to complete
the change.

Elevated Humidity,
Low Water Level Warning

To avoid cell culture desiccation, the
SANYO Sterisonic
maintains ~95% RH at 37°C.

• Humidication is achieved by a com­bined forced-air and natural evapora­tion method enhanced by the Direct
Heat and Air Jacket
protected by an optical water level
indicator to warn of low water in the
removable humidity pan.

• The humidity pan removes easily for
regular cleaning and rell.

• When lled, the pan slides into place.
The optical sensor returns to position
and the SafeCell
any contaminants introduced during
the process.

• For the H
cycle the humidity pan is repositioned
against the chamber wall to eliminate
the need for separate autoclaving.

™

GxP CO2 incubator

™

base heater, and

™

UV lamp destroys

in situ decontamination

2O2

5

Sterisonic™ GxP Control System

Intelligent Interface Through Integrated LCD Control with Graphical Display—The Sterisonic™ GxP incubator is managed by an integrated micro­processor controller with LCD graphical display to simplify all incubator functions. Stable temperature and humidity conditions are achieved through
a combination of performance systems supervised by the controller complete with alarm, programming, calibration and diagnostic protocols.

Control Features

• The Sterisonic™ GxP control and
information center includes an intuitive
pop-up menu, high resolution LCD
for inputs, outputs and performance
at-a-glance.

• Multi-point data logging offers push­button graphical display. An optional PC
interface permits remote transmission
for GMP/GLP protocols as required.

• Selected performance histories can
be acquired in graphical format for
easy interpretation.

• Precise PID logic controls and adjusts
to all temperature and CO

setpoints

2

and alarm parameters.

• A range of setpoint, alarm and program­mable inputs are established through
pop-up menus and function keys.

• Standard parameters are factory-set for
quick start-up, and all parameters can
be changed as required.

• Logged parameters can be exported
to remote databases, off-site alarm or
data capture systems through optional
communications board for compliance
monitoring; see Accessories.

• A remote alarm terminal mounted
at the rear of the cabinet can be
connected to an external alarm system.

• Tactile feedback, touch pad data shift
and entry keys simplify operation.

• The control panel is center mounted
in the outer door for easy access and
viewing.

Decontamination Cycle

The H2O2 decontamination cycle is
monitored for safety and cycle status.
A physical interlock and neutralization
sequence assures total decontamination
and operator safety.

1

Start Cycle: When the H2O2 button is pressed
a confirming message prompts the user to proceed
with the decontamination cycle or cancel.

2

H2O2 Vapor Cycle: Once the door locks auto­matically, the cycle starts. The flashing H2O2 display
confirms the process and counts down remaining
H2O2 vaporization time.

3

UV Resolution: The H2O2 atomizer automatically
completes after a 7 minute cycle. UV lamp comes ON.
The flashing UV Resolve display counts down remaining
time in the UV cycle as H2O2 is reduced to water and
trace oxygen.

4

Cycle Complete: When the cycle is complete the
door lock releases automatically. The H2O2 atomizer and
cable can be disconnected and removed and all interior
components restored to their normal position.

Sterisonic™ GxP H202

Decontamination Cycle

(3 Hours, total)

Elapsed Time:

Conventional High Heat

Decontamination

(24 Hours, total)

Sterilize: 7 min.

SANYO H2O2 atomizer creates vapor which is
circulated throughout chamber by interior blower.

PREP: 15 min.

Reposition shelves, humidity
pan and plenum inside
SANYO chamber. Interior
surfaces are exposed.

Start Cycle: 30 min.

Press H2O2 start button.
Chamber warms to 45˚C.

15 min. 30 min. 45 min. 1 2

Resolve: 90 min.

UV lamp glows for 90 minutes,
reducing H

to harmless water droplets.

2O2

Finish.

Shelves, humidity pan and plenum
are returned to operating position.

3

4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

PREP: 15 min.

Remove interior components
sensitive to high heat.

Start Cycle: 90 min.

Interior chamber elevates to high heat.

Sterilize: 14 hours

Interior chamber remains at high heat.

6 Sterisonic™ GxP Series Cell Culture Incubators www.sanyobiomedical.com

Sterisonic™ GxP Control System

• Digital alphanumeric LCD display.

• Message display

• Pop-up menu

1

2

3

4

• Visual alarm indicator

decontamination sequence

• H

2O2

start key

• Menu call button

• Display contrast adjustment

• Positive feedback tactile

input buttons

• Positive feedback tactile

entry and function keys

Finish.

Incubator must cool from high heat
temperatures to near ambient.

7

Sterisonic™ GxP Design and Technology

All SANYO CO2 incubators feature patented Direct Heat and Air Jacket™ temperature control for accurate, uniform temperature control and
inCu saFe™ for continuous contamination control. SANYO laboratory CO2 incubators feature selected SafeCell™ UV with exclusive, patented
Active Background Contamination Control™.

Direct Heat and Air Jacket™ Heating System

Zone Location Function Energy Microprocessor Controller

Main
(Red)

Base
(Yel low )

Side, top and rear walls Dominant heat source Variable

Floor

Base heater elevates the humidity reservoir
to achieve 95% RH at 37 °C

Variable

Energizes any, all or

in combination of heating

elements as required.

Front
(Green)

Air Jacket
(White)

Insulation
(Gray)

Outer door

Side, top and rear walls

Side, top and rear walls, door

Direct Heat and Air Jacket™
Heating System

The patented Direct Heat and Air

™

Jacket

surrounds the inner walls with a
natural convection airow that converts
to radiant wall heat through thermal con­duction to achieve accurate, uniform and
highly responsive temperature control
within the chamber.

• The microprocessor controller directs

proportional distribution of power to
independent heating sources sur­rounding the chamber.

• Arranged in three zones, these

sources include the side, top and
rear walls, the chamber base and
the outer door.

• Each zone is controlled by the micro-

processor which manages continuous
feedback from the incubator chamber
sensors.

Warms the inner glass in response to ambient conditions;
eliminates condensation on glass and around the opening
and promotes temperature uniformity

Sealed, surrounds interior chamber with natural
air convection and promotes chamber wall uniformity

Promotes energy efciency, mitigates effect of
ambient temperature uctuations on air jacket

The patented Direct Heat and Air Jacket™ heating
system distributes proportional energy to the interior
chamber through a natural convection air jacket
surrounded by a high density insulation to protect
against ambient temperature fluctuations

Variable

– –

– –

H2O2 Decontamination

The unique Sterisonic™ GxP H2O2

decontamination system limits
downtime to less than three hours when
total chamber decontamination is desired.

• All interior components and CO

sampling

2

loop are sterilized in situ; no need for
removal and autoclaving.

• Available as standard on the

MCO-19AIC(UVH) and MCO-19M(UVH);
optional on the MCO-19AIC(UV) and
MCO-19M(UV).

• Following decontamination, SANYO Active

Background Contamination Control

™

ghts
contamination while cell culture protocols
are in process.

SafeCell™ UV System

Sterisonic GxP Models –UVH

and –UV include SafeCell
contamination control technology based on
an integrated combination of narrow band­width, ozone-free ultraviolet light, ceramic
infrared CO

control, inCu saFe™ copper-

2

enriched stainless steel alloy interiors and
Direct Heat and Air Jacket

™

aged by a microprocessor controller.

™

UV

heating man-

8 Sterisonic™ GxP Series Cell Culture Incubators www.sanyobiomedical.com

Sterisonic™ GxP Design and Technology

These incubators offer the industry's
most stable cell culture environment
and are useful for the most critical
applications where continuous contami­nation control is essential to cell viability.
SANYO SafeCell

™

UV Series CO2 incuba­tors offer signicant economic benets
by minimizing interruptions for decon­tamination, by improving cell culture
growth and expression under stable,
repeatable conditions, and by minimizing
the potential for product loss.

• SafeCell

™

UV includes a programmable
ultraviolet lamp, isolated from cell
cultures, that sterilizes conditioned air
and humidity reservoir water to pre­vent contamination without affecting
cell cultures in vitro.

• SafeCell

™

UV inhibits the growth of
mycoplasma, bacteria, molds, spores,
yeasts and fungi without costly HEPA
lter air scrubbers which accumulate
contaminants in the chamber air.

• High temperature decontamination
systems, which can actually encour­age in vitro growth of heat resistant
thermophilic and hyperthermophilic
microorganisms, are avoided.

• inCu saFe

™

interior surfaces provide

natural resistance to contamination.

• The SafeCell

™

UV lamp reduces H2O2
to trace sterile water condensate
following the seven-minute vapor
decontamination process.

Dual Wavelength Infrared
CO2 Control System

The SANYO single beam, dual
detector infrared CO
unprecedented control accuracy and
stability by simultaneously measuring
two wavelengths.

The chamber sample and a reference
is congured around a ceramic-based
sensor linked to the microprocessor
controller with a sophisticated P.I.D./R
(proportional, integral and derivative) algo­rithm. Benets include ultra-fast recovery
without overshoot and accurate CO
ages during periods of frequent incubator
access with multiple door openings.

system offers

2

™

aver-

2

• The ceramic-based technique is imper­vious to changes in temperature and
relative humidity, highly stable during
door openings, and sensitive to resto­ration of setpoint equilibrium when the
door is closed.

• The sensor maintains a continuous
auto-zero function for accuracy.

• Actual CO

is displayed on the main

2

control panel.

• A CO

sample port mounted on the

2

incubator front permits convenient
conrmation of chamber CO

• An optional automatic CO

density.

2

switchover

2

system is available; see Accessories.
A two-stage regulator from the supply
cylinder to the incubator is required;
see Accessories.

MCO-19M Series
Multi-Gas Performance
O2 Control System

In addition to precise CO
MCO-19M Series incubator delivers
exceptional O

control in support of cell

2

culture processes which require below­ambient or above-ambient oxygen levels.
These include in vitro fertilization (IVF),
gene research, regenerative medicine
and emerging applications that are sensi­tive to oxygen levels other than ambient.

• A zirconia oxygen sensor maintains
sub-ambient O

levels from 1% to

2

18%. Additionally, enriched O
from 22% to 80% are enabled with
proper safety precautions.

• Concurrently the MCO-19M permits
a CO

range 0% to 20% via infrared

2

sensor.

• Nitrogen gas bubbler accelerates
recovery of chamber humidity levels
following door openings.

• An electronic P.I.D. control maintains
accurate temperature and gas set­points over the entire system range.

• The MCO-19M includes an automatic
gas switchover system that changes
from the primary to a secondary gas
cylinder for either oxygen or nitrogen;

control, the

2

levels

2

an optional second gas switchover
system is available for CO

.

2

• Cabinet based on MCO-19 platform
with inCu safe™ copper-enriched
stainless steel chamber, continu­ous contamination control, patented
D.H.A. multi-point, air-jacketed temper­ature control system, elevated relative
humidity with integral water-level
sensor.

inCu saFe™ Construction
for Germicidal Protection

SANYO offers exclusive use
of inCu saFe
steel alloy interior surfaces within
a technical design created to eliminate
contamination sources and to mitigate
the effect of airborne contaminates
introduced through normal use.

• Selected to provide natural germicidal

protection without rust or corrosion,
inCu saFe
micidal attribute to inhibit the growth
of molds, fungi, mycoplasma and
bacteria.

• All interior components, including

the air management plenum, shelf
supports, humidity pan and blower
wheel assembly are easily removable
without tools if required.

• During the H

cycle interior components can be
repositioned within the chamber for in
situ decontamination.

• When components are removed, all

interior surfaces are exposed for con­ventional wipe down.

• Large curve corners and electropol-

ished surfaces are easy to clean.

• Pass thru ports accommodate probes

or instrumentation leads as required
for specialized cell culture protocols.
Each chamber includes a port posi­tioned in the interior, rear wall, upper
left, with dual, non-VOC silicone stop­pers inside and outside the cabinet for
added protection.

™

copper-enriched stainless

™

expresses a natural ger-

decontamination

2O2

9

Sterisonic™ GxP Design and Technology

Active Background
Contamination Control

A continuous Active Background Con­tamination Control
contamination without downtime.

At the base of the plenum an isolated
beam of high intensity, ozone-free
ultraviolet light destroys contaminants
in the air and in the humidity water res­ervoir, away from active cell cultures.

™

process eliminates

™

SafeCell™ UV Lamp Program Cycles

Mode Function

After H

2O2

Vaporization

After Door
Opening

OFF If UV protection is not desired.

24 Hour
Continuous ON

The UV lamp automatic ally cycles ON for up to 90 minutes following the seven-minute

H2O2 vapor cycle, reducing the H2O2 to water droplets. These droplets automatically

condense onto a naturally cooler section of the interior oor for easy wipe -up.

UV lamp automatically ON for ve minutes after door is closed decontaminates
incoming room air.

Useful for overnight decontamination prior to rst use, clinical decontamination protocols
between patients, or following total chamber wipe-out af ter maintenance or ser vice.

• Contaminants trapped within the
distilled water pan are destroyed by
ultraviolet light.

• Sterile, humidied air is released
from the lower plenum for vertical con­vection through and around the per­forated shelves. Interior air motion is
suspended when the door is opened,
minimizing movement of room air
contaminants into the chamber.

• UV light is isolated by the plenum
cover to protect cell cultures

• Airborne contaminants are eliminated
by an automatic UV cycle that au­tomatically turns ON for a specied
period after each door opening.

• Trace contaminants that attach to
interior surfaces are destroyed by the
passive germicidal properties of the
inCu saFe

™

surfaces.

The SafeCell™ UV lamp cycle is factory set for normal use, and can be re-programmed as desired by entering
parameters through the central microprocessor control panel. Program parameters for the H2O2 decontamination cycle
are non-adjustable for operator safety.

Mycoplasma Survival Results

Mycoplasma
Strain

Mycoplasma
fermentans PG18

Mycoplasma
orale CH19299

Mycoplasma
arginini G230

Mycoplasma
hominis PG21

Chart summarizes test results with four strains of mycoplasma. Results demonstrate how SANYO InCu saFe™
copper enriched stainless steel alloy offers germicidal properties of conventional C1100 copper while maintaining
both corrosion-proof and discoloration-resistant properties of conventional Type 304 stainless steel.
Detailed test results are available from SANYO.

Positive

Control

survival no survival survival survival

survival no survival survival survival

survival no survival survival survival

survival no survival survival survival

Conventional Type

304 Stainless Steel

SANYO

InCu saFe

™

Conventional

Copper C1100

10 Sterisonic™ GxP Series Cell Culture Incubators www.sanyobiomedical.com

Sterisonic™ GxP Specifications

Sterisonic™ GxP Series Energy, Electrical and Utilties

Description

MCO-19AIC

MCO-19AIC(UV)

MCO-19AIC(UVH)

MCO-19M

MCO-19M(UV)

MCO-19M(UVH)

Maximum Power Consumption 310W 310W

Maximum Heat Discharge 1062 BTU 1062 BTU

Electrical Connection

Gas Connection 4 to 6 mm inner diameter tubing 4 to 6 mm inner diameter tubing

CO

2

CO

Gas Input Pressure Nominal 4.3 psi from two- stage CO2 regulator Nominal 4.3 psi from two- stage CO2 regulator

2

CO

Gas Cylinder Switchover System Optional Optional

2

O

Control System N/A Microprocessor PID, Zirconia Sensor

2

O

Range and Variation N/A 1-18%, 22-8 0%

2

N

Inlet Connect/ Pressure N/A Nominal 7 psi from two-stage regulator

2/O2

N

Switchover System N/A Standard

2/O2

115V,60Hz, 1 phase, NEMA 5 -15P plug provided;

requires NEMA 5-15R grounded receptacle.

115V,60Hz, 1 phase, NEMA 5 -15P plug provided;

requires NEMA 5-15R grounded receptacle.

Sterisonic™ GxP Series Dimensions, Weights and Capacities

Model Number

MC O-19AI C
MCO-19AIC(UV)
MCO-19AIC(UVH)

MC O-19 M
MC O-19 M(UV )
MC O-19 M(UV H)

Volume

(cu.ft.)

6.0

170 L

6.0

170 L

Exterior Dimensions

(w x f-b x h)

24.4" x 27.9" x 35.4"

620 x 710 x 900 mm

24.4" x 27.9" x 35.4"

620 x 710 x 900 mm

Interior Dimensions

(w x f-b x h)

19.3" x 20.6" x 26 .2"

490 x 523 x 665 mm

19.3" x 20.6" x 26 .2"

490 x 523 x 665 mm

Shelves Net Weight

15 max / 4 supplied std.

17.7 " x 17.7 " (15.4 lbs capacity)

450 x 450 mm (7 kg capacity)

15 max / 3 supplied std.

17.7 " x 17.7 " (15.4 lbs capacity)

450 x 450 mm (7 kg capacity)

(nominal)

205 lbs

93 kg

207 lbs

94 kg

4.5"

(115 mm)

4.9"
(125 mm)

FRONT SIDE

24.4" (620 mm)

19.3" (490 mm)

22.8" (580 mm)

26.2" (665 mm)

34.6" (880 mm)

0.8"
(20 mm)

24.8" (630 mm)

20.6" (523 mm)

18.4" (469 mm)

3.1"

(80 mm)

1.9" (49 mm)

1.3" (34 mm)

1.7" (44 mm)

11

Sterisonic™ GxP CO2 Series Specifications

Sterisonic™ GxP CO2 Incubators

Description

Single Chamber Model Number MCO-19AIC(UVH) MCO-19AIC(UV) MCO-19AIC

Dual Chamber Model Number MCO-38AIC(UVH) MCO-38AIC(UV) MCO-38AIC

Major Operating Systems

H2O2 Decontamination System Standard Optional Optional

™

SafeCell

UV System Standard Standard Optional

Single Beam, Dual Detector IR CO

inCu saFe

™

Copper Enriched Stainless Steel Interior Standard Standard Standard

LCD Graphical Controller/ Display, Door Mounted Standard Standard Standard

Direct Heat, Air

™

(DHA ) Air Jacket Standard Standard Standard

Decontamination

H

Decontamination System Vaporization in situ Optional Optional

2O2

Interior UV Lamp, Programmable, Ozone Free Standard Standard Optional

Copper Enriched Stainless Steel Interior
with Germicidal Protection

Sensor Standard Standard Standard

2

Standard Standard Standard

Environmental Performance

Temperature Control Range +5ºC above ambient to 50°C (in a 5ºC to 35ºC ambient)

Temperature Control Uniformity Deviation ±0.25ºC (in 25ºC ambient, setting 37 ºC, 5% CO

CO

Control Range and Deviation 0% to 20%, ±0.15% in 25ºC ambient, set ting 37 ºC, 5% CO2, no load

2

CO

Sensor Platform

2

Calibration Single point zero automatic; semi- automatic one point span @ reference optional.

CO

2

Ceramic based, single beam, dual wavelength measurement

of actual vs. contrast, with continuous auto-zero calibration.

, no load)

2

Airow Gentle vertical air ow, continuous with inner door closed.

Interior Humidity 95%RH @ 37°C through evaporation via DHA

™

heating system; reective optical low water sensor.

Control, Monitoring, Alarm

Temperature and CO

Control P.I.D., setpoint resolution 0.1% and 0.1°C

2

Display Alphanumeric LCD digital display messaging.

Data Acquisition Data Acquisition Automatic log function of temperature and CO

Communications

Optional PC interface, Catalog No. MTR- 4 80 with RS232/RS4 85 data ports available

Remote alarm contacts standard. Optional 4-20mA connection.

.

2

Cabinet Design and Construction

Interior, Shelves 4, Copper-enriched stainless steel.

Inner Door Tempered glass.

Insulation Rigid foam polyurethane.

Outer Door Reversible, heated.

Access Port Single opening with interior and exterior 1.18" (30 mm) non -VOC silicone stoppers.

Leveling Feet 4, adjustable.

12 Sterisonic™ GxP Series Cell Culture Incubators www.sanyobiomedical.com

Sterisonic™ GxP CO2/O2 Series Specifications

Sterisonic™ GxP CO2/O2 Incubators

Description

Single Chamber Model Number MCO-19M(UVH) MCO-19M(UV) MCO-19 M

Dual Chamber Model Number MCO-38M(UVH) MCO- 38M(UV ) MCO -38M

Major Operating Systems

H2O2 Decontamination System Standard Optional Optional

™

SafeCell

Single Beam, Dual Detector IR CO

inCu saFe

LCD Graphical Controller/ Display, Door Mounted Standard Standard Standard

Direct Heat, Air

Decontamination

H

Interior UV Lamp, Programmable, Ozone Free Standard Standard Optional

Copper Enriched Stainless Steel Interior
with Germicidal Protection

UV System Standard Standard Optional

Sensor Standard Standard Standard

2

™

Copper Enriched Stainless Steel Interior Standard Standard Standard

™

(DHA ) Air Jacket Standard Standard Standard

Decontamination System Vaporization in situ Optional Optional

2O2

Standard Standard Standard

Environmental Performance

Temperature Control Range +5ºC above ambient to 50°C (in a 5ºC to 35ºC ambient)

Temperature Control Uniformity Deviation ±0.25ºC (in 25ºC ambient, setting 37 ºC, 5% CO

CO

Control Range and Deviation 0% to 20%, ±0.15% in 25ºC ambient, set ting 37 ºC, 5% CO2, no load

2

CO

Sensor Platform

2

Calibration Single point zero automatic; semi-automatic one point span at reference (optional).

CO

2

O

Control Range and Deviation 1-18%, 22-80%, ±0.2% in 25º C ambient, setting 37º C, no load

2

O

Sensor Platform Zirconia sensor, microprocessor P.I.D. control

2

Ceramic based, single beam, dual wavelength measurement

of actual vs. contrast, with continuous auto-zero calibration.

, no load)

2

Airow Gentle vertical air ow, continuous with inner door closed.

Interior Humidity 95%RH @ 37°C through evaporation via DHA

™

heating system; reective optical low water sensor.

Control, Monitoring, Alarm

Temperature and CO

Control P.I.D., setpoint resolution 0.1% and 0.1°C

2

Display Alphanumeric LCD digital display messaging.

Data Acquisition Data Acquisition Automatic log function of temperature and CO

Communications

Optional PC interface, Catalog No. MTR- 4 80 with RS232/RS4 85 data ports available

Remote alarm contacts standard. Optional 4-20mA connection.

.

2

Cabinet Design and Construction

Interior, Shelves 3, Copper-enriched stainless steel.

Inner Door 4 separate, gasketed inner doors, tempered glass

Insulation Rigid foam polyurethane.

Outer Door Reversible, heated.

Access Port Single opening with interior and exterior 1.18" (30 mm) non -VOC silicone stoppers.

Leveling Feet 4, adjustable.

13

Sterisonic™ GxP CO2 Series Accessories

SANYO Biomedical products include a broad range of accessories to meet specific application requirements. For accessories or options
not listed herein, contact SANYO or your authorized SANYO sales representative.

Sterisonic™ GxP CO2 Series Incubators

Single Chamber Model Number

Dual Chamber Model Number

H

Decontamination K it Built-in MCO-HL N / A

2O2

H

Vapor Atomizer MCO-HP N/A N / A

2O2

H

Reagent (Formulated for SAN YO Sterisonic™ GxP) MCO- H2O2 N/A N / A

2O2

Automatic CO

Gas Calibration System, semi-automatic one point calibration function. MCO-SG MCO-SG MCO-SG

CO

Cylinder Regulator, CGA tting 320 M CO -10 0L M CO -10 0L MCO-10 0 L

2

Roller Base. For use in single or stacked installations. MCO-18 RB MC O -18R B MCO -18 RB

inCu saFe

inCu saFe

Integrated Cooling Option MCO-CL MCO-CL MCO- CL

Communications Port. Located at rear of chamber.
Connector, cable and software not supplied.

Communications Port. Located at rear of chamber, analog 4 -20 mA . MCO-420MA MCO-420MA MCO- 420MA

SafeCell

Cylinder Switchover System M CO - 21GC MCO-21G C M CO- 21G C

2

™

Shelf and Brackets. Includes two shelf brackets. Full shelf MCO- 47S T M CO -47ST MCO- 47S T

™

Half Tray System MCO-25ST MCO-25ST MCO -25ST

™

UV System Kit Narrow-bandwidth 253.7nm lamp and assembly. Built-In Built- In MCO -19 UV S

MCO-19AIC(UVH)

MCO-38AIC(UVH)

Catalo g No. Catalog No. C atalog No.

MTR-480 MTR- 480 MTR-480

MCO-19AIC(UV)

MCO-38AIC(UV)

MCO-19AIC

MCO-38AIC

H2O2 Reagent (MCO-H2O2)

SANYO H2O2 solution is specially formulated for optimal
use with the MCO-HP atomizer. Each pre-measured
bottle is sufficient for a complete H2O2 decontamination
sequence. Unit of issue: six per carton.

H2O2 Vapor Atomizer (MCO-HP)

Shown with connecting cable, standard.

Integrated Cooling Coil (MCO-CL)

Factory installed; specify when ordering. Water bath/
circulator not included. Permits stable operation at
ambient or below ambient temperatures.
Extends performance specifications as follows:

• Temperature Range: +18°C to +50°C, distribution
+/-0.25°C, variation +/-0.1°C.

• Relative Humidity: 5°C to +50°C, 95% +/- 5%RH; 20°C
to 25°C >80%RH; 18°C >70%RH

Includes temperature mapping results for individual
unit per serial number.

Relative Temperature Performance

+50ºC +50ºC

With

Cooling Coil

+18 ºC

Without

Cooling Coil

+29ºC over

Ambient

Ambient
Temperature
+24ºC

14 Sterisonic™ GxP Series Cell Culture Incubators www.sanyobiomedical.com

Sterisonic™ GxP CO2/O2 Series Accessories

Sterisonic™ GxP CO2/O2 Series Incubators

Single Chamber Model Number

Dual Chamber Model Number

H

Decontamination K it Built-in N /A N / A

2O2

H

Vapor Atomizer MCO-HP N/A N / A

2O2

H

Reagent (Formulated for SAN YO Sterisonic™ GxP) MCO- H2O2 MCO- H2O2 N / A

2O2

Automatic CO

Cylinder Switchover System M CO - 21GC MCO-21G C M CO- 21G C

2

Gas Calibration System, semi-automatic one point calibration function. MCO-SG MCO-SG MCO-SG

CO

Cylinder Regulator, CGA tting 320 M CO -10 0L M CO -10 0L MCO-10 0 L

2

N

Cylinder Regulator, CGA tting 5 80 (for low oxygen applications) MC O -100N MCO -10 0N MCO -10 0N

2

O

Cylinder Regulator, CGA tting 5 40 (for high oxygen applications) MCO-10 0 M M CO -10 0 M MCO-100 M

2

Roller Base. For use in single or stacked installations. MCO-18 RB MC O -18R B MCO -18 RB

inCu saFe

inCu saFe

™

Shelf and Brackets. Includes two shelf brackets. Full shelf MCO- 47S T M CO -47ST MCO- 47S T

™

Half Tray System MCO-25ST MCO-25ST MCO -25ST

Integrated Cooling Option MCO-CL MCO-CL MCO- CL

Communications Port. Located at rear of chamber.
Connector, cable and software not supplied.

Communications Port. Located at rear of chamber, analog 4-20 mA . MCO-420MA MCO-420MA MCO- 420MA

™

SafeCell

UV System Kit Narrow-bandwidth 253.7nm lamp and assembly. Built-In Built- In MCO -19 UV S

MCO-19M(UVH

MCO-38M(UVH)

MCO-19M(UV)

MCO-38M(UV)

MCO-19M

MCO-38M

Catalo g No. Catalog No. C atalog No.

MTR-480 MTR- 480 MTR-480

Integrated Cooling Option

Feature

Temperature Range +18ºC to +50 ºC, distribution + / -0.25 ºC, variation +/ - 0.1ºC

Relative Humidity 5ºC to +50ºC, 9 5% +/ - 5%RH; 20ºC to 25ºC > 80%RH; 18ºC > 70%RH

Includes temperature mapping results for individual unit per serial number

Components Stainless Steel Cooling Coil, Interconnection Lines, 6 L, Refrigerated Water Bath & Circulator (Optional / Not Provided )

15

Service and Technical Support

Unique SANYO Services

• On-site consultation

• Specialized documentation for each
individual unit

• Customized testing procedures
based on personalized customer
requirements

SANYO Connect

SANYO’s customer-driven biomedi­cal service program guarantees local
attention from qualied SANYO service
representatives, whenever and wher­ever you need it.

• New Unit Installation and Training

• Preventative Maintenance

• Warranty and Non-Warranty Repairs

• Calibration/Validation Services

• Refurbishment and Reconditioning

• Customized Service and Warranty
Programs

• In-Stock Parts for Immediate Delivery

Predelivery and On-Site Services

Predelivery services include factory
acceptance testing, calibration, and
temperature mapping. On-site services
include installation qualication,
operational qualication, performance
qualication, calibration and temperature
mapping.

Product conforms to RoHS
(European Restriction of Hazardous
Substance directives)

SANYO Electric Co.,Ltd., Biomedical Division, Gumma is certied for quality management
system:ISO9001/medical devices quality management system:ISO13485/environmental
management system:ISO14001

™

UV U.S. Patent 6255103; Direct Heat and Air Jacket™ U.S. Patent 5519188;

SafeCell
SafeCell™ UV, inCu saFe™, Direct Heat and Air Jacket™, P.I.D./R™ and Active Background
Contamination Control™, are trademarks of SANYO Electric Biomedical Co., Ltd.

© 2010 Specifications subject to change without notice.

SANYO North America Corporation

1300 Michael Drive, Suite A, Wood Dale, IL 60191

Toll free USA (80 0) 858-8442, Fax [phone]

Biomedical Solutions Division

www.sanyobiomedical.com

101910 V6