Sanyo MCO-19AIC User Manual
INSTRUCTION MANUAL
MCO-19AIC㩿UV㪀
MCO-19AIC
CO2 Incubator
91
CONTENTS
INTRODUCTION 2
PRECAUTIONS FOR SAFE OPERATION 3
LABELS ON THE INCUBATORS 7
ENVIRONMENTAL CONDITIONS 7
INCUBATOR COMPONENTS 8
Control panel and keypad 10
Remote alarm terminals 11
INSTALLATION SITE 12
INSTALLATION 13
Connection of CO
PREVENTING CONTAMINATION 15
PRECAUTIONS FOR CULTURES 16
Using the unlock key 17
CORRECT OPERATION 17
LCD PANEL 18
BASIC OPERATIONS ON CONTROL PANEL 19
BASIC PARAMETERS 20
Setting the chamber temperature and CO
Setting the key lock 21
Removing the key lock 21
Setting the key lock password 22
Setting the upper limit alarm temperature 24
ALARM PARAMETERS 25
UV LAMP PARAMETERS 26
Using the UV lamp 26
Precautions when using the UV lamp 27
Setting the UV lamp ON period 28
Lighting the UV lamp for 24 hours 29
OTHER PARAMETERS 30
Setting the date, time, and log interval 30
Initial settings (LCD/DAQ parameters) 32
DISPLAYING THE LOG 33
Transferring data 34
WATER LEVEL SENSOR 35
ROUTINE MAINTENANCE
Cleaning the chamber and inner attachments 36
Filling the humidifying pan 39
H
DECONTAMINATION 40
2O2
H
Precautions when handling H
ALARMS, SAFETY, AND SELF-DIAGNOSIS 46
CALIBRATION
Temperature/CO
TROUBLESHOOTING 50
DISPOSING OF THE CO
AUTOMATIC CO
AUTOMATIC CO
STACKING INCUBATORS 62
SPECIFICATIONS 64
PERFORMANCE 65
SAFETY CHECK SHEET 66
decontamination 42
2O2
gas cylinder 14
2
density 20
2
decontamination reagent 45
2O2
calibration 48
2
INCUBATOR 52
2
CYLINDER CHANGEOVER 57
2
DENSITY CALIBRATION 59
2
1
92
INTRODUCTION
Ŷ Read this manual carefully before using the Product and follow the instructions for safety operation.
Ŷ Sanyo disavows any responsibility for safety if the Product is used for other than the intended use or
used with any procedures other than those given in this manual.
Ŷ Keep this manual in a suitable place so that it can be referred to as necessary.
Ŷ The contents of this manual are subject to change without notice for improvement of performance or
functions.
Ŷ Contact a Sanyo sales representative or agent if any page of the manual is lost or the page order is
incorrect.
Ŷ Contact a Sanyo sales representative or agent if any point in this manual is unclear or if there are any
inaccuracies.
Ŷ No part of this manual may be reproduced in any form without the expressed written permission of
Sanyo.
93
2
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with this manual as it contains
important safety advice.
Items and procedures are described so that you can use this unit correctly and safely.
If the precautions advised are followed, this will prevent possible injury to the user and
any other person.
Precautions are illustrated in the following way:
WARNING
Failure to observe WARNING signs could result in a hazard to personnel
possibly resulting in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.
Symbol shows;
Be sure to keep this manual in a place accessible to users of this unit.
< Label on the unit >
this symbol means caution.
this symbol means an action is prohibited.
this symbol means an instruction must be followed.
This mark is labeled on the cover in which the electrical components of high voltage
are enclosed to prevent the electric shock.
The cover should be removed by a qualified engineer or a service personnel only.
WARNING
As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity
of the equipment. It is important that you assess the work site to ensure there is suitable and
sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a
safe environment must be considered. These may include atmosphere monitoring and
warning devices.
3
94
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to
rain water.
Only qualified engineers or service personnel should install the unit. The installation by
unqualified personnel may cause electric shock or fire.
Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from
turning over. If the floor is not strong enough or the installation site is not adequate, this may result
in injury from the unit falling or tipping over.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
Never install the unit in a flammable or volatile location. This may cause explosion or fire.
Never install the unit where acid or corrosive gases are present as current leakage or electric
shock may result due to corrosion.
Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not
grounded, it will be necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such
grounding may cause electric shock in the case of an incomplete circuit.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use
of any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit if the container cannot be sealed. These
may cause explosion or fire.
Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.
This may cause electric shock or injury by accidental contact with moving parts.
Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use
may cause bad effect on your health or environment.
Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any
repair or maintenance of the unit in order to prevent electric shock or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.
WARNING
95 4
WARNING
Ensure you do not inhale or consume medication or aerosols from around the unit at the time of
maintenance. These may be harmful to your health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put containers with liquid on the unit as this may cause electric shock or short circuit when
the liquid is spilled.
Never bind, process, or step on the power supply cord, or never damage or break the power
supply plug. A broken supply cord or plug may cause fire or electric shock.
Do not use the supply cord if its plug is loose. Such supply cord may cause fire or electric shock.
Never disassemble, repair, or modify the unit yourself. Any such work carried out by an
unauthorized person may result in fire, or electric shock or injury due to a malfunction.
Disconnect the power supply plug if there is something wrong with the unit. Continued
abnormal operation may cause electric shock or fire.
When removing the plug from the power supply outlet, grip the power supply plug, not the cord.
Pulling the cord may result in electric shock or fire by short circuit.
Disconnect the power supply plug before moving the unit. Take care not to damage the power
cord. A damaged cord may cause electric shock or fire.
Disconnect the power plug when the unit is not used for long periods. Keeping the connection
may cause electric shock, current leakage, or fire due to the deterioration of insulation.
If the unit is to be stored unused in an unsupervised area for an extended period, ensure that
children do not have access and that doors cannot be closed completely.
The disposal of the unit should be accomplished by appropriate personnel. Remove doors to
prevent accidents such as suffocation.
Do not put the packing plastic bag within reach of children as suffocation may result.
Use the reagent specified by Sanyo for H
may result in explosion or damage to the Incubator.
When performing H
do so may be harmful to health due to leakage of H
During H
to do so may be harmful to health due to leakage of H
decontamination, plug the access hole with the silicon cap that is provided. Failure
2O2
decontamination, securely close the internal and external doors. Failure to
2O2
decontamination. Using a different H2O2 solution
2O2
gas.
2O2
gas.
2O2
5
96
Use a dedicated power source (a dedicated circuit with a breaker) as indicated on the rating label
attached to the unit. A branched circuit may cause fire resulting from abnormal heating.
Connect the power supply plug to the power source firmly after removing the dust on the plug.
A dusty plug or improper insertion may cause a heat or ignition.
Never store corrosive substances such as acid or alkali in this unit if the container cannot be
sealed. These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet when you request any repair or maintenance for the safety of service
personnel.
Wear rubber gloves when handling the H
result in inflammation of the skin.
H
decontamination can be performed only for the chamber and chamber attachments with
2O2
standard specifications, and not for any other objects.
Perform H
Arranging them in a different way may result in insufficient decontamination.
After H
to wipe off the residual H
decontaminated, and the bottoms of ducts.
2O2
decontamination with the chamber attachments arranged as specified by Sanyo.
2O2
decontamination has been completed, wear rubber gloves and use a non-woven cloth
2O2
CAUTION
reagent. Direct contact with the H2O2 reagent may
2O2
fluid from the bottom of the chamber, any objects that were
97
6
LABELS ON THE INCUBATOR
Warning and caution labels are attached to the Incubator. The following table describes the labels.
This label is attached to covers that access high-voltage electrical components to
prevent electric shock. Only a qualified engineer or service personnel should be
allowed to open these covers.
This symbol indicates an ultraviolet light (UV) caution.
This symbol indicates that caution is required. Refer to product documentation for
details.
This symbol indicates a hot surface.
This symbol indicates an earth.
This symbol means “ON” for a power switch.
This symbol means “OFF” for a power switch.
EMVIRONMENTAL CONDITIONS
The Indicator is designed to be safe if used under the following conditions (based on the IEC 61010-1):
Ŷ Indoors
Ŷ Altitudes up to 2000 m
Ŷ Ambient temperature from 5 to 40
Ŷ Maximum humidity of 80% for temperatures up to 31
50% at 40
Ŷ Main power supply voltage fluctuations of r10% of the nominal voltage
Ŷ Other power supply voltage fluctuations as stated by the manufacturer
Ŷ Transient overvoltages according to Installation Category II
For the main power supply, the minimum and normal category is II.
Ŷ Pollution degree 2 according to IEC 60664.
o
C
o
C
o
C decreasing linearly to maximum humidity of
7
98
INCUBATOR COMPONENTS
17
1
6
22
Rear Right Side
12
5
10
21
For MCO-19AIC(UV) or
when MCO-19UVS is installed.
9 (inside)
7
13 (inside)
20
14
8
3
2
4
15, 16 (inside)
For MCO-19AIC(UV) or
when MCO-19UVS is installed.
18
11
19
Rear Left Side
99
8
1. Outer door: The outer door is held to the frame with a magnetic seal. A door heater is installed in the
door panel. The door opening is reversible. Contact a Sanyo representative or agent to change the door
hinge from left to right or vice versa.
2. Inner door: The inner door is made of tempered glass. However do not subject the glass to excessive
impacts.
3. Leveling feet: The leveling feet can be turned to adjust the height. Adjust the feet so that the Incubator
is level.
4. Trays: The trays can be pulled forward.
5. Tray supports: The tray supports can be removed by lifting the front side and pulling toward you.
6. Side supports: The right and left side supports can be removed for disinfection. Refer to page 36 and
37.
7. Fan cover: The fan cover serves as the inlet for circulating air. It is removable.
8. Duct: The duct for the path for circulating air. It is removable.
9. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an autoclave.
10. Sample air outlet: The sample air outlet also functions as an internal gas outlet. Normally, cover this
outlet with the sample air outlet cap.
11. Connecting port A/B for CO
Changeover System is installed, both ports A and B are available. If the MCO-21GC is not installed, only
port A is available. Refer to page 14 for gas cylinder connection. Ensure that the gas pressure is set at
0.03 MPaG (0.3 kgf/cm
12. Door switch: The door switch detects when the door is open and stops the circulating fan and
electromagnetic valve for CO
13. Humidifying pan: Fill the humidifying pan with sterile distilled water. Install the pan properly so that it
can be covered with the pan cover.
14. Humidifying pan cover: The cover prevents UV light entering the chamber. When filling the pan, lift
the front side and take out the pan. Refer to page 39 for details.
15. UV lamp*: This Sanyo UV lamp does not generate ozone. Never look directly at the UV light. For
replacement, contact a Sanyo representative or agent.
16. Water level sensor for humidifying pan: This sensor detects the water level in the humidifying pan.
Refer to page 35 for details.
17. Remote alarm terminals: Refer to page 11.
18. Access port: Place the silicon caps on the port both outside and inside when the port is not being
used.
19. Power switch: This is the main switch for the Incubator. It also functions as an overcurrent breaker.
2
G, 4.3 psiG). Refer to page 57 for automatic CO2 cylinder changeover system.
2
gas pipe: When the optional MCO-21GC Automatic CO2 Cylinder
2
and UV lamp.*
20. Glow starter*: The glow started if for the UV lamp (model FG-7P)
21. Attachment location for electric key: This is the attachment location for the electric key included in
the optional Component H
decontamination. Refer to the installation procedure for MCO-HL for details.
22. Sample air outlet cap: Always attach this cap except when using the sample air outlet.
* MCO-19AIC(UV) or when an optional UV System Kit (MCO-19UVS) is installed.
9
Decontamination Kit (MCO-HL). This kit must be attached to perform H2O2
2O2
100
Control panel and keypad
1
3
2
5
10
1. LCD panel
2. Upper limit regulator:
This regulator is used to set the upper
temperature limit.
3. OVERHEAT indicator:
This indicator lights when the chamber
temperature reaches the upper limit.
4. MENU Key:
Press this key to access the menu.
5. Contrast knob:
Turn this knob to adjust the contrast of the
LCD.
6. BUZZER Key:
Press this key to silence the buzzer.
Note:
buzzer for an upper limit temperature
alarm.
㶎 The following optional components must be installed to perform decontamination.
䊶UV Lamp Add-on Kit (MCO-19UVS) (This is provided as standard equipment for the MCO-19AIC(UV).)
䊶H
䊶H
6
It is not possible to silence the
Decontamination Kit (MCO-HL)
2O2
Generator (MCO-HP)
2O2
7. CE Key:
Press this key to clear the entered value
when entering a setting.
8. Cursor Keys (Up, Down, Left, and Right):
Use these keys to move the cursor on the
LCD panel.
9. ENTER Key:
Press this key to select a menu command.
When entering a setting, pressing this key to
move to the next parameter.㩷
10. Numeric Keys
11. H
Press this key to start H
Refer to page 40 for details.
2O2
Key:
2O2
11
7
decontamination.
4
8
9
101
10
Remote alarm terminals
The remote alarm terminals are located at the rear right side.
The remote alarm terminals are a contact output.
Normal: Open
Alarm: Closed
Contact capacity: 30 VDC, 2 A
Note:
• When the power switch is OFF or power is interrupted, the contact output will be closed.
• The remote alarm cannot be silenced by pressing the BUZZER Key since the remote alarm is not
conjunct with the BUZZER Key.
Remote alarm terminals
REMOTE ALARM
MAX DC30V 2A
Remote alarm
terminals
N.O.
11
102
INSTALLATION SITE
For correct operation of the Incubator, install it in a location with the following conditions.
WARNING
When using CO2 gas for control, make sure that there is adequate ventilation. Using CO2 gas in a
small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition,
when opening the Incubator doors, do not directly inhale the air in the chamber.
Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO
être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la
porte.
Normal air environment
z
Install the Incubator in an environment with normal air.
Do not expose to direct sunlight
z
Do not install the Incubator in a location where it will be exposed to direct sunlight. If the Incubator is
operated in direct sunlight, performance will be adversely affected.
Separate from heat sources
z
Do not install the Incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves.
Heat will adversely affect the performance of the Incubator.
Ambient temperature at least 5qC lower than set temperature
z
The control temperature of the Incubator is at least 5qC higher than the ambient temperature. For
example, if the chamber is controlled at 37qC, the ambient temperature must normally be no more than
32qC. Do not allow the ambient temperature to become too high.
Strong and level floor
z
Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the Incubator
will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure
that the installation is stable. An unstable surface may result in vibration or noise.
de l’air peut s’élever et peut
2
WARNING
Install the Incubator at a location that can support the weight. If the floor is not strong enough or if the
installation is insufficient, the Incubator may fall over and cause injury.
Always make sure that the floor is strong, even, and level, and that the Incubator will not tip over.
An insufficient installation may result in injury due to water leakage or the Incubator falling over.
Low humidity
z
Select a site with a relative humidity of 80% or lower. Using the Incubator in high humidity may result in
current leakage or electric shock.
WARNING
Do not use the Incubator outdoors. If the Incubator is exposed to rain water, it may result in current
leakage or electric shock.
Never install the Incubator in a moist location, such as near a sink or water line, or where it is
likely to be exposed to water. In addition, do not install it near water or steam pipes. Moisture can
the insulation to deteriorate, which may result in current leakage or electric shock.
cause
No inflammable or corrosive gas
z
Never install the Incubator in a location where it will be exposed to inflammable or corrosive gas. Doing
so may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective
casing, resulting in current leakage or electric shock.
No falling objects
z
Do not install the Incubator in a location where there is the possibility of objects falling from above. Doing
so may result in damage or accident.
103
12
INSTALLATION
1. Remove the packing tape and clean up.
Remove all the tape that is securing the doors and inner attachments. Open the doors for ventilation. If
the outer panels are dirty, dampen a cloth with a diluted neutral detergent and wipe them. (Using an
undiluted solution may damage the plastic. Follow the diluting instructions for the detergent that is used.)
Wipe off the residual detergent with a wet cloth and then wipe off any moisture.
2. Adjust the leveling feet.
Adjust the leveling feet by turning them counterclockwise to
level the
Incubator. (See the illustration on the right.)
3. Ground the Incubator.
Ground the Incubator during installation to prevent electric
shock in case the insulation is not sufficient. If there is no
ground wire at the location, consult with qualified service
personnel.
When a ground must be installed
z
If a grounded 3-pole outlet is not available, then a ground must be installed. Consult with qualified service
personnel.
Leveling feet
WARNING
To prevent electric shock, always ground the Incubator. If grounding is not possible, then have a ground
installed by qualified personnel. If the Incubator is not grounded, it may result in electric shock.
Never connect the ground wire to a gas pipe, water pipe, lightning rod, or telephone ground wire.
Doing so may cause electric shock
Installing a ground fault circuit breaker
z
If using the Incubator in a location with moisture or humidity cannot be avoided, then it is recommended
that a ground fault circuit breaker be installed in the power supply circuit (i.e., the power supply at the
Incubator). Have the circuit breaker installed by qualified service personnel.
.
CAUTION
Do not climb on the Incubator or place objects on top of it. Doing so may damage it or cause it to fall
over, resulting in injury. If it is to be stacked, refer to page 62 and stack it securely.
When the Incubator is not in use
z
Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the
chamber is completely dry before closing the doors. Failure to do so may result in damage.
Before moving the Incubator
z
Before moving the Incubator, empty the water from the humidifying pan, disconnect the power supply plug
from the outlet, and make sure that the cord will not be damaged. Failure to do so may result in electric
shock or fire.
13
104
Connecting a CO2 gas cylinder
WARNING
When connecting a gas cylinder to the Incubator, confirm the gas type. Confirm that the connections
are secure and that no gas will escape. Be sure to use the specified pressure. Using an incorrect
gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to escaping
gas.
Install the Incubator in a location with adequate ventilation. If adequate ventilation cannot be
provided, then install an alarm system using
1. Use a liquefied CO
2. Install the Gas Pressure Regulator (MCO-100L, purchased separately) specified by Sanyo on the CO
gas cylinder. Otherwise, use a CO
for the primary pressure, and 0.2 MPaG (2 kgf/cm
3. Using the gas supply pipe that is provided, connect the CO
inlet (located at the lower left-hand side on the rear panel of the Incubator).
Note:
If CO
is supplied to multiple CO2 Incubators from a single gas cylinder, a CO2 solid will be formed in the
2
gas pressure regulator. The gas pressure regulator safety valve will operate, and there may an explosive
sound.
CO2 and O2 densitometers.
gas cylinder (at least 99.5% pure). The siphon (dip tube) type cannot be used.
2
gas pressure regulator rated at 25 MPaG (250 kgf/cm2G, 3600 psiG)
2
2
G, 30 psiG) for the secondary pressure.
2
gas pressure regulator to the CO2 gas pipe
2
When the MCO-21GC is not mounted
Using the gas supply pipe that is provided, connect the CO
cylinder to CO
sure that no gas is leaking.
gas pipe connection port A on the Incubator. After connecting the pipe, check to make
2
gas pressure regulator for the CO2 gas
2
When the MCO-21GC is mounted
Connect a pair of CO
will be switched automatically. Connect the main cylinder to port A and the reserve cylinder to port B. After
connecting the cylinders, check to make sure that no gas is leaking.
gas cylinders with CO2 gas pressure regulators independently. The gas supply line
2
For details on installing the optional automatic CO
z
MCO-21GC installation guide. For details on using the MCO-21GC, refer to page 57.
Cylinder Changeover Kit (MCO-21GC), refer to the
2
4. Set the CO2 gas on the secondary side to 0.03 MPaG (0.3 kgf/cm2G, 4.3 psiG) for gas injection. As the
pressure increases, the CO
cause gas supply lines inside the Incubator to come loose, which may result in gas poisoning or oxygen
deprivation due to the escaping of gas. If gas lines come loose, the Incubator must be repaired.
The gas lines connected to the Incubator will degrade over time. If any deterioration or abnormalities
z
are found during inspection, replace the lines immediately.
gas concentration control range will increase. Excessive pressure may
2
105
14
PREVENTING CONTAMINATION
To prevent contamination of the chamber, select a suitable installation site.
Avoid locations with high temperatures or humidity.
z
Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in
the air.
Avoid locations with passers-by or drafts.
z
Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry
of microorganisms into the chamber.
If possible, use a cleanroom.
z
To achieve a better culture, it is recommended that a cleanroom be used if one is available.
Use clean containers.
z
The greatest cause of contamination is dirty containers for cultures. Be careful not to get containers or
trays dirty when taking them in and out.
Keep the chamber clean.
z
Wipe off any fingerprints. If water spills from the humidifying pan, or if the doors are left open for a long
time, condensation may form on the inside of the doors. If that occurs, wipe off the condensation with a
dry sterile gauze. In particular, clean and disinfect the chamber if the culture medium is spilled. For details,
refer to Routine Maintenance on page 36.
Use sterile distilled water in the humidifying pan.
z
Always use sterile distilled water in the humidifying pan. Do not use ultrapure water, because it may
contain red rust-like suspended particles.
A water level alarm is displayed in the status display area on the control panel. Quickly refill the water in
the pan when the water level alarm is displayed. Adding low-temperature water will significantly lower the
temperature in the chamber. Clean the humidifying pan once a month.
Keep the Incubator out of direct airflows from air conditioners or fans.
z
Cool airflow from an air conditioner may cause condensation and lead to possible contamination.
15
106
PRECAUTIONS FOR CULTURES
Leave space between culture containers.
z
Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate
spacing may result in uneven temperature distribution and CO
Do not place harmful materials in the chamber.
z
Never place samples that release acidic, alkali, or corrosive gas in the chamber. Doing so may cause
damage resulting from discoloration or corrosion.
Close the inner door.
z
Always close the inner door before closing the outer door. Failure to close the inner door will adversely
affect performance even if the outer door is closed.
Open and close the doors gently.
z
Always open and close the doors gently. Closing the doors forcefully may cause spillage of the culture
medium, incomplete closing, or damage to the gasket. Before opening the inner door, check through the
glass to confirm that the UV lamp is OFF (if the MCO-19AIC(UV) or the optional MCO-19UVS is
installed).
Be careful when closing the outer door.
z
Use the handle when closing the outer door. Holding the door in other places may cause injury by getting
fingers caught in the door. Do not lean on the outer door. Doing so may result in injury from the outer door
coming loose or the Incubator falling over, or it may cause current leakage or electric shock.
Be careful of the inside of the outer door.
z
The inside of the outer door may become hot.
Avoid using excessive force on the inner door.
z
Do not put your hand on the glass, poke it with sharp objects, or apply strong force. Doing so may result
in injury from breaking the glass.
Check the cause of any alarm buzzer.
z
If an alarm buzzer sounds while the Incubator is in use, immediately check the cause of the alarm. For
details on what may cause an alarm buzzer to sound, refer to page 46.
gas concentration.
2
107
16
Using the Unlock Key
Unlocking when power is interrupted
z
If power is interrupted to the MCO-19AIC(UV)/19AIC with an MCO-HL installed, the outer door is
electrically locked. To unlock the outer door while the power is interrupted, use the unlock key that is
provided. To lock the outer door again, turn the unlock key to the lock direction while the door is open.
After the door has been locked condition manually, then close the door.
Note:
The outer door cannot be locked by using the unlock key while the door is closed. Lock the door while it is
open. Attempting to turn the unlock key while the door is closed may damage the electric lock system.
WARNING
For the MCO-19AIC(UV)/19AIC with an MCO-HL installed, the outer door is electrically locked during
decontamination. The chamber is filled with H
during decontamination.
㩷
gas during decontamination. Do not unlock the door
2O2
CORRECT OPERATION
Use the following procedure to start trial operation or actual operation of the Incubator.
1. Install the Incubator correctly, referring to Installation on page 13.
2. Remove the packing materials from the chamber and inner attachments. Clean and disinfect the
chamber and inner attachments, referring to Routine Maintenance on page 36.
3. Add approximately 1.5 L of sterile distilled water to the humidifying pan. (Refer to page 39.)
4. Turn ON the power supply switch on the left side of the rear panel of the Incubator.
Note:
The humidity in the Incubator chamber is adjusted to the optimum setting. To prevent condensation on
the surface inside chamber and the inner door, there is a low-temperature area under the humidifying pan
in the bottom of chamber to recondense evaporated moisture. Condensation may occur around the
humidifying pan at the bottom of the chamber (on the inside of the humidifying pan cover), but this does
not indicate a problem.
WARNING
Do not leave plastic wrapping bags within reach of children. If a bag is placed over a child’s head, it
can block the mouth and nose and cause suffocation.
17
108
LCD PANEL㩷
㩷
The following display (called the Top Display) will appear when the power switch is turned ON. The
default temperature is 37.0
factory. Refer to page 30 to change the date and time.㩷㩷
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2
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1. Display of set value of temperature
The set value of chamber temperature is displayed.
2. Display of current temperature
The current chamber temperature is displayed.
3. Display of set value of CO
The set value of the chamber CO
4. Display of current CO2 density
The current chamber CO
5. Display of current CO
A and B will be displayed if the optional Automatic CO
installed. The port of the CO
Nothing will be displayed if the MCO-21GC is not installed.
6. Status display field
Various status or alarms are displayed.
䊶When UV lamp is lit: “UV” is displayed in reverse video.
䊶When humidifying water is low: “RH PAN” is displayed in reverse video.
䊶When the door is open: “Door: Open” is displayed in reverse video.
7. Message display field
A message is displayed when fault occurs. The message is displayed alternately in normal characters
and reverse video. Refer to pages 46 and 47 for alarm details. “OK” is displayed during normal
operation. 㩷
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1
01 0
2
T e m p 3 7 . 0 o C C O 2 5 . 0 % A B
㧟㧣㧜
S t at u s U V R H
O K
7
1
2
3
4
5
6
7
o
C and the default CO2 density is 0%. The date and time are preset at the
3
11 12 13 1415161718192021222324252627282930 31 32 33 34 35 363738394
㧡㧜
A N
P
density
2
density is displayed.
2
density is displayed.
2
cylinder
2
Cylinder Changeover System (MCO-21GC) is
2
gas pipe that is currently supplying CO2 will be displayed in reverse video.
2
5
D o o r : O p e n
0
4
109
18
BASIC OPERATIONS ON CONTROL PANEL㩷
㩷
The following operation are possible through control panel:㩷
㩷
1. Setting the temperature䊶䊶䊶The chamber temperature can be set (page 20).
2. Setting the CO
3. Setting the key lock䊶䊶䊶Changing the temperature and CO
4. Setting alarms䊶䊶䊶The temperature alarm, CO
temperature (page 24) can be set.
5. Setting UV parameters䊶䊶䊶ON/OFF parameters and the ON period of the UV lamp can be set for the
MCO-19AIC(UV) or when the Incubator is equipped with the optional MCO-19UVS (page 26).
6. Setting other parameters䊶䊶䊶The initial settings of the date, time, and log cycle (page 30), and the
LCD display and baud rate (page 32) can be set.
7. Displaying the operation log and transferring data䊶䊶䊶A graph of past operation data can be
displayed (page 33) and operation data can be transferred to a PC (page 34).
8. H
decontamination䊶䊶䊶H2O2 decontamination of the chamber and internal attachments can be
2O2
performed when the optional H
9. Automatic CO
Cylinder Changeover System (MCO-21GC) is installed (page 57).
CO
2
10. Setting the CO
optional Automatic CO
㩷
density䊶䊶䊶The chamber CO2 density can be set (page 20).
2
density can be disabled (page 21).
2
density alarm (page 25), and upper limit alarm
2
Generator (MCO-HP) is installed (page 40).
2O2
cylinder changeover䊶䊶䊶The cylinder can be switched when the optional automatic
2
standard gas density䊶䊶䊶The CO2 standard gas density can be set when the
2
Standard Gas Calibration Kit (MCO-SG) is installed. (page 59)
2
19
110
BASIC PARAMETERS
㩷
㩷 Setting the chamber temperature and CO2 density㩷
㩷
The setting procedure for the chamber temperature and CO
Chamber temperature: 37
o
C, CO2 density: 0%)㩷
The Incubator automatically starts operation using these settings after the power is turned ON.
㩷
1. From the Top Display, press the MENU Key to access the menu. Select Set and press the ENTER
Key.
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1
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2
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3
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4
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5
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6
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7
m p 3 7 . 0o C C O 2 5 . 0 %
T e
㧟㧣㧜
S t a t u s
O K
㧡㧜
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2. The Stand-By Setting Display will be displayed. Set the parameters.㩷
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1
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2
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3
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4
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5
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6
S t a n d - by S e t t ing
T e m p e r a t u r e
C O 2 D e n s i t y 5 . 0 %
K e y L o c k 0
H i g h L i m i t 5 2 . 0
3 7 . 0oC ( 0 . 0oC- 5 0 . 0 o C )
( 0 . U n l o c k 1 . L o c k )
o
C
㩷
㩷
The setting ranges of the parameters are as follows:
ƔTemperature: 0 to 50
ƔCO
density: 0 to 20%. To set 5.0%, enter 050.㩷
2
o
C. To 37.0oC, enter 370.
㩷
3. Press the MENU Key to display the menu after setting the parameters. Select OK and press the
ENTER Key. The settings will be saved.
㩷
Note:
When starting the Incubator for the first time or after not using it for an extended period of time, allow at
least 4 hours for the chamber temperature, humidity, and CO
desired chamber temperature at a 0% CO
density. Then change the setting to the desired CO2 density.
2
㩷
㩷
㩷
density are given below. (Default settings:㩷
2
M E N U
S e t
L o g
T o o l s
M E N U
O K
(0.0% - 20. 0 % )
sensor to become stable after setting the
2
C a n c e l
111
20
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