Sanyo MCO-18AC9UV) Installation Manual

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INSTRUCTION MANUAL

CO2 Incubator

MCO-18AC(UV MCO-18AC

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CONTENTS

INTRODUCTION P. 2

PRECAUTIONS FOR SAFE OPERATION P. 3

LABELS ON THE INCUBATORS P. 6

ENVIRONMENTAL CONDITIONS P. 7

INCUBATOR COMPONENTS P. 8

Control panel and keypad P. 10

Remote alarm terminal P. 11

INSTALLATION SITE P. 12

INSTALLATION P. 13

Connection of CO

PREVENTING CONTAMINATION P. 15

PRECAUTIONS FOR CULTURES P. 16

START-UP OF INCUBATOR P. 17

SETTING THE CHAMBER TEMPERATURE AND CO

UV LAMP

Precautions when using the UV lamp P. 20

Setting the UV lamp ON period P. 21

Lighting the UV lamp for 24 hours P. 22

KEY LOCK FUNCTION P. 23

WATER LEVEL SENSOR

ALARMS, SAFETY, AND SELF-DIAGNOSIS P. 25

SETTING OF ALARM RESUME TIME P. 26

Operation after power failure P. 26

ROUTINE MAINTENANCE

Cleaning the chamber and inner attachments P. 27

Filling the humidifying pan P. 30

CALIBRATION

Temperature calibration P. 31

calibration P. 32

TROUBLESHOOTING P. 33

DISPOSING OF THE CO

AUTOMATIC CO

STACKING INCUBATORS P. 40

SPECIFICATIONS P. 42

PERFORMANCE P. 43

SAFETY CHECK SHEET P. 44

gas cylinder P. 14

(MCO-18AC(UV) or MCO-18AC with MCO-18UVS3)

INCUBATOR P. 34

CYLINDER CHANGEOVER P. 39

DENSITY P. 18

P. 19

P. 24

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INTRODUCTION

■ Read this manual carefully before using the appliance and follow the instructions for safety operation.

■ Sanyo never guarantee any safety if the appliance is used for any objects other than intended use or

used by any procedures other than those mentioned in this manual.

■ Keep this manual in an adequate place to refer to it as necessary.

■ The contents of the manual will be subjected to change without notice due to the improvement of

performance or functions.

■ Contact Sanyo sales representative or agent if any page of the manual is lost or page order is incorrect.

■ Contact Sanyo sales representative or agent if any point in this manual is unclear or if there are any

inaccuracies.

■ No part of this manual may be reproduced in any form without the expressed written permission of

Sanyo.

CAUTION

SANYO guarantees the product under certain warranty conditions. SANYO in no way shall be responsible for any loss of content or damage of content.

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PRECAUTIONS FOR SAFE OPERATION

It is imperative that the user complies with this manual as it contains important safety advice.

Items and procedures are described so that you can use this unit correctly and safely. If the precautions advised are followed, this will prevent possible injury to the user and any other person.

Precautions are illustrated in the following way:

WARNING

Failure to observe WARNING signs could result in a hazard to personnel possibly resulting in serious injury or death.

CAUTION

Failure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property.

Symbol shows;

this symbol means caution.

this symbol means an action is prohibited.

this symbol means an instruction must be followed.

Be sure to keep this manual in a place accessible to users of this unit.

< Label on the unit >

This mark is labeled on the cover in which the electrical components of high voltage are enclosed to prevent the electric shock. The cover should be removed by a qualified engineer or a service personnel only.

WARNING

As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity of the equipment. It is important that you assess the work site to ensure there is suitable and sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a safe environment must be considered. These may include atmosphere monitoring and warning devices.

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WARNING

Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to

rain water.

Only qualified engineers or service personnel should install the unit. The installation by

unqualified personnel may cause electric shock or fire.

Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from

turning over. If the floor is not strong enough or the installation site is not adequate, this may result

in injury from the unit falling or tipping over.

Never install the unit in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

Never install the unit in a flammable or volatile location. This may cause explosion or fire.

Never install the unit where acid or corrosive gases are present as current leakage or electric

shock may result due to corrosion.

Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not

grounded, it will be necessary to install a ground by qualified engineers.

Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

Connect the unit to a power source as indicated on the rating label attached to the unit. Use

of any other voltage or frequency other than that on the rating label may cause fire or electric shock.

Never store volatile or flammable substances in this unit if the container cannot be sealed. These

may cause explosion or fire.

Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.

This may cause electric shock or injury by accidental contact with moving parts.

Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use

may cause bad effect on your health or environment.

Turn off the power switch (if provided) and disconnect the power supply to the unit prior to

any repair or maintenance of the unit in order to prevent electric shock or injury.

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet

hand. This may cause electric shock.

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WARNING

Ensure you do not inhale or consume medication or aerosols from around the unit at the time of

maintenance. These may be harmful to your health.

Never splash water directly onto the unit as this may cause electric shock or short circuit.

Never put containers with liquid on the unit as this may cause electric shock or short circuit when

the liquid is spilled.

Never bind, process, or step on the power supply cord, or never damage or break the power

supply plug. A broken supply cord or plug may cause fire or electric shock.

Do not use the supply cord if its plug is loose. Such supply cord may cause fire or electric shock.

Never disassemble, repair, or modify the unit yourself. Any such work carried out by an

unauthorized person may result in fire, or electric shock or injury due to a malfunction.

Disconnect the power supply plug if there is something wrong with the unit. Continued

abnormal operation may cause electric shock or fire.

When removing the plug from the power supply outlet, grip the power supply plug, not the cord.

Pulling the cord may result in electric shock or fire by short circuit.

Disconnect the power supply plug before moving the unit. Take care not to damage the power

cord. A damaged cord may cause electric shock or fire.

Disconnect the power plug when the unit is not used for long periods. Keeping the connection

may cause electric shock, current leakage, or fire due to the deterioration of insulation.

If the unit is to be stored unused in an unsupervised area for an extended period, ensure that

children do not have access and that doors cannot be closed completely.

The disposal of the unit should be accomplished by appropriate personnel. Remove doors to

prevent accidents such as suffocation.

Do not put the packing plastic bag within reach of children as suffocation may result.

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CAUTION

Use a dedicated power source (a dedicated circuit with a breaker) as indicated on the rating label

attached to the unit. A branched circuit may cause fire resulting from abnormal heating.

Connect the power supply plug to the power source firmly after removing the dust on the plug.

A dusty plug or improper insertion may cause a heat or ignition.

Never store corrosive substances such as acid or alkali in this unit if the container cannot be

sealed. These may cause corrosion of inner components or electric parts.

Check the setting when starting up of operation after power failure or turning off of power

switch. The stored items may be damaged due to the change of setting.

Be careful not to tip over the unit during movement to prevent damage or injury.

Prepare a safety check sheet when you request any repair or maintenance for the safety of service

personnel.

LABELS ON THE INCUBATORS

Warning and caution labels are attached to the incubator. The following table describes the labels.

This label is attached to covers that access high-voltage electrical components to prevent electric shock. Only a qualified engineer or service personnel should be allowed to open these covers.

This symbol indicates an ultraviolet light (UV) caution.

This symbol indicates that caution is required. Refer to product documentation for

details.

This symbol indicates a hot surface.

This symbol indicates an earth.

This symbol means “ON” for a power switch.

This symbol means “OFF” for a power switch.

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ENVIRONMENTAL CONDITIONS

This equipment is designed to be safe at least under the following conditions (based on the IEC

61010-1):

■ Indoor use;

■ Altitude up to 2000 m;

■ Ambient temperature 5

■ Maximum relative humidity 80% for temperature up to 31

at 40

■ Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage;

■ Other supply voltage fluctuations as stated by the manufacturer;

■ Transient overvoltages according to Installation Categories (Overvoltage Categories) II; For mains

supply the minimum and normal category is II;

■ Pollution degree 2 in accordance with IEC 60664.

C to 40oC

C decreasing linearly to 50% relative humidity

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INCUBATOR COMPONENTS

Handles

9 (inside)

19,20 (inside)

For MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

Handles

10, 11

For MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

Rear Right Side

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Rear Left Side

Page 10

1. Outer door: Sticks to frame with magnetic seal. Door heater is installed in the outer door panel. The door opening is reversible. Contact Sanyo representative or agent to change the door hinge from left to right or vice versa.

2. Inner door: Made of tempered glass, however avoid excessive impact on the glass.

3. Leveling foot: Screw type for adjusting the height. Adjust the foot so that the incubator can be

level.

4. Tray: Can be pulled toward you.

5. Tray support: Can be removed by lifting the front side and pulling toward you.

6. Side support: Right and left side supports can be removed for disinfection. See page 27, 28 and

29.

7. Fan cover: Inlet of circulating air. Removable.

8. Duct: Flow path for circulating air. Removable.

9. Fan (inside the rear duct): Made from polypropylene resin. Can be disinfected by an autoclave.

10. Sample air outlet: This also functions as an internal gas outlet. Normally, cover this outlet with

the sample air outlet cap.

11. Sample air outlet cap: Always attach this cap except at the time of using of sample air outlet.

Note: Operation without sample air outlet cap may result low humidity or condensation on the outer door.

12. Connecting port for CO

gas pipe: When an optional component MCO-21GC (automatic CO

cylinder changeover system) is installed, both A and B are available. If MCO-21GC is not used, only A is available. Refer to page 14 for gas cylinder connection. Ensure that the gas pressure is set at 0.03 MPaG (0.3 kgf/cm

G, 4.3 psiG). Refer to page 39 for automatic CO2 cylinder changeover system.

13. Door switch: Detects the door opening/closing and stops the circulating fan, UV lamp* and electromagnetic valve for CO

when door is open.

14. Humidifying pan: Use sterile distilled water to fill the pan. (MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed) Install the pan properly so that it can be covered with the pan cover.

15. Remote alarm terminal: Refer to page 11.

16. Access port: When not in use, cap with the silicon caps on both outside and inside.

17. Power switch: Main switch of the incubator. Also functions as an over-current breaker. The

switch is covered by a switch cover to prevent the accidental push. To turn on or off the switch, remove the switch cover by loosening the screw.

18. Humidifying pan cover*: Prevents UV light being exposed to the chamber. When filling the pan, lift the front side and take out the pan. See page 30 for details.

19. UV lamp*: Sanyo UV lamp does not generate ozone. Never look at the UV light directly. For replacement, contact Sanyo sales representative or agent.

20. Water level sensor for humidifying pan*: Detects the water level in the humidifying pan. See page 24 for details.

21. Glow starter*: For UV lamp (model; FG-7P)

*MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

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Control panel and keypad

3 4 5 6 7

10 11

151617

1. Digital temperature indicator (TEMPERATURE

C): Normally, this indicator shows the chamber temperature. In the setting mode, it shows the set value of the chamber temperature. If the self diagnostic function detects any abnormality, an error code will be displayed.

2. Heater lamp (HEAT): This lamp lights when the heater is energized.

3. UV indicator (UV): This lamp lights when the UV lamp is ON. The blink of this indicator

recommends the replacement of UV lamp. See page 19 for the details. MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

4. Door lamp (DOOR): This lamp lights when the outer door is open.

5. Water level alarm lamp (RH PAN): This lamp blinks when the water in the humidifying pan is less

than approximately 0.8 liter. MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

6. Upper limit regulator: This regulator is used to set the upper temperature limit.

7. Over heat lamp (OVER HEAT): This lamp lights when the chamber temperature reaches the upper

limit set value.

8. CO

inject lamp (INJECT): This lamp lights when CO2 gas is being injected.

9. Digital CO

chamber. In the setting mode, it indicates the set value of the CO mode, the decimal point in the digital CO

density indicator (CO2 %): Normally, this indicator shows the CO2 concentration in the

concentration. In the calibration

density indicator blinks.

10. Set key (SET): Pressing this key to enter the setting mode, and the digits to be set will blink.

11. Calibration key (CAL): Pressing this key to enter the CO

calibration pre-conditioning mode.

Normally when the outer door is opened, the fan motor will stop operating. This mode allows the fan

motor to continue to circulate the chamber environment to ensure accurate CO

uniformity inside the

chamber. At this time, by pressing this key for approximately 5 seconds, the incubator enters the

temperature calibration input mode. And then pressing this key again, the incubator enters the CO

calibration input mode. See page 31 and 32 for the details. Also, used to change the UV lamp ON

period. See page 21 for the details.

Note:

This operation affects the basic performance. When calibrating the CO

density, contact a Sanyo sales

representative or agent.

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Page 12

12. Alarm buzzer stop key (BUZZER): Press this key to silence the buzzer when the alarm operates and the buzzer sounds (The remote alarm is not stopped).

13. CO

provided that MCO-21GC automatic CO

14. CO

an automatic CO is empty, the CO

gas supply line indicator (A/B): The lamp for the supply line currently in use lights up

cylinder changeover system (option) is installed.

gas supply line switching key : This key to select CO2 gas supply line is available only when

cylinder changeover system MCO-21GC (option) is installed. When one CO2cylinder

is supplied by the other cylinder automatically.

15. Enter key (ENT): Pressing this key memorizes the set value in the controller.

16. Numerical value shift key ( ): Pressing this key in the setting mode causes the numerical value

to shift. In key lock mode, pressing this key makes key lock ON or OFF. See page 23 for details.

17. Digit shift key ( ): Pressing this key in the setting mode causes the changeable digit to shift. Pressing this key more than 5 seconds enters key lock mode. See page 23 for the key lock.

Remote alarm terminal

The terminal of the remote alarm is installed at the rear right side of the incubator. The alarm is

outputted from this terminal. Contact capacity is DC 30 V, 2 A.

Contact output:

between COM. and N.O. between COM. and N.C.

At normal Open Close

At abnormal Close Open

Note:

The alarm is actuated when the power cord is disconnected from the outlet or the power switch is OFF.

• The remote alarm cannot be stopped by pressing the alarm buzzer stop key (BUZZER) since the

remote alarm is not conjunct with the alarm buzzer stop key (BUZZER).

Remote alarm terminal

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INSTALLATION SITE

To operate this incubator properly and to obtain maximum performance, install the incubator in a location with the following conditions:

WARNING

Ventilate a room air occasionally when using CO2 gas for control. The gas density will increase in

an enclosed small room and high level of gas density can be hazardous to health. In addition, avoid

inhaling the chamber air directly when opening the door if CO

Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO

être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la

porte.

■ A location not subjected to direct sunlight

Do not install the incubator under direct sunlight. Installation in a location subjected to direct sunlight

cannot obtain the intended performance.

■ A location away from heat generating sources

Avoid installing the incubator near heat-emitting appliances such as a heater or a boiler etc. Heat can

decrease the intended performance of the incubator.

gas is used.

de l’air peut s’élever et peut

■ 5

C higher than the ambient temperature

The chamber temperature must be at least 5

chamber temperature is set to 37

ambient temperature in adequate range.

■ A location with a sturdy and level floor

Always install the incubator on a sturdy and level floor. The uneven floor or tilted installation may cause

failure or injury. Install the incubator in stable condition to avoid the vibration or noise. Unstable

condition may cause vibration or noise.

C, the ambient temperature must be less than 32oC. Keep the

C higher than the ambient temperature. For example, the

WARNING

Install the incubator on a sturdy floor. If the floor is not strong enough or the installation site is not

adequate, this may result in injury from the incubator falling or tipping over.

Select a level and sturdy floor for installation. This precaution will prevent the incubator from tipping.

Improper installation may result in water spillage or injury from the incubator tipping over.

■ A location not prone to high humidity

Install the incubator in the ambient of 80% R.H. or less humidity. Installation under high humidity may

cause current leakage or electric shock.

WARNING

Do not use the incubator outdoors. Current leakage or electric shock may result if the incubator is

exposed to rain water.

Never install the incubator in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

■ A location without flammable or corrosive gas

Never install the incubator in a flammable or corrosive location. This may cause explosion or fire or may

result in the current leakage or electric shock by the corrosion of the electrical components.

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INSTALLATION

1. Remove the packaging materials and tapes

Remove all transportation packaging materials and tapes. Open the doors and ventilate the incubator.

If the outside panels are dirty, clean them with a neutral detergent and wipe it up with a wet cloth.

WARNING

Do not put the packing plastic bag within reach of children as suffocation may result.

2. Adjust the leveling feet

Extend the leveling feet by rotating them counterclockwise to contact them to the floor. Ensure the

incubator is level.

3. Fix the incubator

Two fixtures are attached to the rear of the frame. Fix the frame to the wall with these hooks and rope or

chain.

4. Ground (earth)

WARNING

Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet is

not grounded, it is necessary to install a ground by qualified engineers.

Never ground the incubator through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

● Stacked module

When the product is used in stacked module, make sure to fix the incubator by using the stacking kit

WARNING

Do not climb onto the incubator and do not put articles on the incubator. This may cause injury by

tipping or damage to the incubator. When stacking the incubator, follow the procedure shown on page

40.

● If not used

When the incubator is not used, dispose the water in the humidifying pan and remove any moisture in the

chamber completely. Check that the chamber is completely dry before closing the doors.

● Before moving the incubator

Empty the humidifying pan completely before moving the incubator. Spilled or splashed water may

cause current leakage or electric shock.

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Connection of CO2 gas cylinder

WARNING

Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are

connected correctly and are not liable to become disconnected. Ensure that the gas pressure is

set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure

may result in leakage of CO

lead to asphyxiation and risk of death.

gas. Elevated level of CO2gas can be hazardous to health and may

1. Use a liquefied CO

gas cylinder, not a siphon (dip tube) type. The CO2gas should be 99.5% or more

pure.

2. Install a gas pressure regulator (optional accessory MCO-100L) on the cylinder. Use a regulator

rated at 25 MPaG (250 kgf/cm

G, 3600 psiG) on the primary side and 0.2 MPaG (2.0 kgf/cm2G, 30 psiG)

on the secondary side.

3. Using the gas supply pipe provided, connect the CO

gas pipe located at the rear left side of the CO2 incubator.

gas pressure regulator to the connecting port for

Note:

If CO

is supplied to multiple CO2 incubators from a single gas cylinder, a CO2 solid will be formed in the

gas pressure regulator. The gas pressure regulator safety valve will operate, and there may an explosive

sound.

When the MCO-21GC is not mounted

Using the gas supply pipe that is provided, connect the CO

cylinder to CO

gas pipe connection port A on the incubator. After connecting the pipe, check to make

gas pressure regulator for the CO2 gas

sure that no gas is leaking.

When the MCO-21GC is mounted

Connect a pair of CO

gas cylinders with CO2 gas pressure regulators independently. The gas supply line

will be switched automatically. Connect the main cylinder to port A and the reserve cylinder to port B. After

connecting the cylinders, check to make sure that no gas is leaking.

4. Set the CO

Excessive pressure may cause disconnection of internal pipes inside the CO

in leakage of CO

pressure on the secondary side to 0.03 MPaG (0.3 kgf/cm2G, 4.3 psiG) (at gas injection).

incubator which will result

gas into the atmosphere. Elevated level of CO2gas can be hazardous to health

and may lead to asphyxiation and risk of death. The repair of the incubator will be necessary if the

internal pipe is disconnected.

4. Check that no gas is leaking at any point where the pipe connects with the CO

regulator or the CO

incubator.

Note:

The incubator, including the gas supply pipes and services must be examined at frequent intervals to

ensure they are safe. Ensure that items such as pipes are replaced if there is any sign of deterioration.

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Page 16

PREVENTING CONTAMINATION

To prevent contamination of the chamber, select an appropriate location for installation as well as the

complete disinfection of the chamber components.

● Avoid hot and humid location

Avoid location with high temperature and/or humidity as the presence of bacteria in the air is greater than

in normal environment.

● Avoid drafty location and location with many passers-by

Avoid locations near doors, air conditioners, fans, etc., where slight breezes can facilitate the entry of

bacteria into the chamber.

● Installation in a sterile room

To get the cultivation more efficiently, install the incubator in a sterile room.

● Use clean containers

The contamination is mainly caused by the containers such as Petri dishes or bottles stored in the

chamber. Always keep the containers clean.

● Always keep the chamber clean

The condensation may be caused on the inside of the door by spilled water form humidifying pan or

opening of outer door for long period. Wipe off the condensation completely with a sterile dry gauze.

Especially when the culture medium is spilled, clean and disinfect the chamber immediately. Refer to

page 27 “Routine maintenance” for details.

● Keep the inside panels dry

To protect the inside of the incubator from contamination, the inside panels should always be kept dry. If

water is spilled from a humidifying pan or if the door is kept open for a long period, condensation will form

on the panels, allowing germs to breed. In such a case, wipe away the water with a dry sterile gauze.

Particularly, if the medium is spilled, wipe it up immediately and sterilize the area.

● Fill the humidifying pan with sterile distilled water

Always use sterile distilled water to fill the pan. The water level alarm lamp (RH PAN) on the control

panel blinks when the water level is low. Refill the sterile distilled water to the pan when the water level

alarm lamp (RH PAN) blinks. Note that when low temperature sterile distilled water is poured, the

chamber temperature drops significantly. Clean the humidifying pan once a month.

● Do not place the incubator in the direct air flow from an air conditioning system

Air from an air conditioning system may cause condensation and lead to possible contamination.

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Page 17

PRECAUTIONS FOR CULTURES

● Do not subject to direct air flow

Do not allow the air for air conditioning to hit the incubator or door directly. Direct hit may cause

condensation or contamination.

● Allow adequate space between the cultures

When storing cultures in the chamber, keep the Petri dishes or bottles containing the cultures sufficiently

apart from each other to allow adequate air circulation. Inadequate space may result in uneven

temperature distribution and CO

● Stored materials

Never place acid or alkaline materials or materials that release corrosive gas in the chamber. Such

materials can cause failure resulting from discoloration or corrosion.

● Always shut the inner door

Shut the inner door completely, and then shut the outer door. If the inner door is not closed completely,

even if the outer door is closed, the incubator will fail to exhibit its maximum performance. And close the

doors gently. Rude closing may cause spillage of medium, incomplete closing, or damage of gasket.

concentration in the chamber.

● Open/close the doors gently

Ensure you close the doors gently. Robust closing may cause spillage of medium, incomplete closing,

or damage of gasket. Also, before opening the inner door, check that the UV light is OFF

(MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed).

● Take care of the inside of the outer door.

It may get hot.

● Never lean or press on the glass.

Broken glass due to intentional force may cause injury.

● Alarm

Always investigate the cause and fix the alarm condition immediately when the alarm is activated. Refer

to page 25 for alarm details.

● Fix the tray supports and trays securely

Incomplete installation may cause injury or damage.

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Page 18

START-UP OF INCUBATOR

When start the test operation or the operation, follow these steps as below.

1. Install the incubator referring to “INSTALLATION” on page 13.

2. Remove all transportation packaging materials and tapes on the attachments in the chamber. Then

clean and sterilize the chamber and inner attachments. Refer to “Cleaning the chamber and inner

attachments” on page 27.

3. Add approximately 1.5 L of sterile distilled water to the humidifying pan. (Refer to page 30.)

4. Turn ON the power switch on the rear left side.

Note:

The humidity in the incubator chamber is adjusted to the optimum setting. To prevent condensation on

the surface inside chamber and the inner door, there is a low-temperature area under the humidifying pan

in the bottom of chamber to recondense evaporated moisture. Condensation may occur around the

humidifying pan at the bottom of the chamber, but this does not indicate a problem.

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Page 19

SETTING THE CHAMBER TEMPERATURE AND CO

DENSITY

Table below shows the basic procedure for setting the chamber temperature and CO

density. The

upper limit alarm temperature setting is also shown in the table. Perform key operations in the sequence

indicated in the table. The example in the table is based on the assumption that the desired temperature

is 37

C and CO2 density is 5%. Adjustment of the upper limit regulator should be executed after the

chamber temperature reaches the stable condition.

Note:

• The incubator is set at the factory so that the chamber temperature is 37

C and CO2 density is 0%.

• When starting the incubator for the first time or after not using it for an extended period of time, allow at

least 8 hours for the chamber temperature, humidity, and CO

desired chamber temperature at 0% CO

density. Then change the setting to the desired CO2 density.

sensor to become stable after setting the

Basic operation sequence (Example: Chamber temperature: 33oC, CO2 density: 5%)

Description of operation Key operated Indication after operation

1 Turn the power switch ON. ----

The current chamber temperature is displayed

in the digital temperature indicator.

2 Press set key. SET The left digit blinks.

By pressing numerical value shift

key and digit shift key,

set the figure to 37.0.

When pressed,

the changeable digit is shifted.

When pressed,

the figure of settable digit changes.

Set temperature is memorized.

4 Press enter key. ENT

By pressing digit shift key and

numerical value shift key, set the

When pressed, the changeable digit is shifted.

figure to 05.0.

Left digit in the digital

density indicator blinks.

When pressed,

the figure of settable digit changes.

6 Press enter key. ENT Set CO

(Executed after the chamber temperature reaches the stable condition)

Adjust upper limit regulator so that

the alarm temp. is 1

C higher than

----

set temperature.

8 Press enter key. ENT

In the digital CO

In the digital temperature indicator, upper limit

temp. is displayed.

The upper limit temp. can be changed

by turning upper limit regulator.

This is the end of set mode and the indicators

display current temperature and CO

density is memorized.

density indicator, HI is displayed.

density.

Note:

● In each set mode, if the change of the setting is not necessary, pressing set key (SET) skips to next set

mode.

● When the CO

density is set to 00.0, the control is OFF regardless of chamber density.

● The upper limit temperature set value will change when the regulator is turned even if the incubator is

not in set mode, because the alarm circuit is an independent circuit.

● In each set mode, the indicator returns to the current temperature and CO

density display mode

automatically when 90 seconds has passed without any key operation.

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Page 20

UV LAMP

The clauses below are applicable when the model is MCO-18AC(UV) or an optional UV system kit

MCO-18UVS3 is installed.

A UV lamp is located inside the duct to sterilize the water in the humidifying pan and air circulating in the

chamber.

Following shows precautions and instructions about the UV lamp.

z When all chamber attachments are installed correctly, only the inside of the duct and the inside of the

humidifying pan cover are exposed to UV light.

z Correctly install all of the chamber attachments when starting a cell culture, and never turn ON the UV

lamp when the humidifying pan cover is removed.

z Always install the humidifying pan cover even when using the incubator without turning ON the UV

lamp. Leaving the cover uninstalled will affect the chamber temperature distribution and the humidity

recovery performance.

(MCO-18AC(UV) or MCO-18AC with MCO-18UVS3)

z The UV lamp stays lit for a preset period of time after the outer door is closed. The default setting is for

5 minutes. The period can be changed when necessary as shown in the page 21.

z If the outer door is not opened for at least 12 consecutive hours, the UV lamp will light for the preset

time period every 12 hours. The period can be changed when necessary as shown in the page 21.

● When checking the UV lamp operation, open the outer door and push the door switch with the inner

door closed. The visible blue light can be checked under the humidifying pan cover. The UV light is

harmful to eyes. Never turn on the UV light with the inner door or humidifying pan cover opened.

z The recommended replacement time for the UV lamp (i.e., when the UV output ratio drops to approx.

70% of its initial value) is when the accumulated ON time reaches 1,000 hours. When the accumulated

ON time reaches approximately 1,000 hours, the UV indicator blinks (when the UV lamp is not lit). It is

recommended that the UV lamp be quickly replaced at this point. Contact a Sanyo sales representative or

agent for information on replacing the UV lamp.

z If the UV lamp burns out, E18 is displayed on the digital temperature indicator. If this occurs, replace

the UV lamp. When replacing the UV lamp, replace the glow starter (type FG-7P) at the same time.

Contact a Sanyo sales representative or agent for information on replacing the UV lamp.

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Page 21

Precautions when using the UV lamp

The clauses below are applicable when the model is MCO-18AC(UV) or an optional UV system kit

MCO-18UVS3 is installed.

WARNING

Do not look directly at UV light. UV light is harmful to the eyes.

Always use the humidifying pan and humidifying pan cover.

The humidifying pan and cover prevent UV light from escaping. Always use them even when not

humidifying. To check whether the UV lamp is lit, open the outer door and then press the door switch with

the inner door still closed. Visible blue light can be confirmed from the front of the humidifying pan cover.

UV light is harmful to the eyes, so do not light the UV lamp when the inner door or humidifying pan cover

is open.

Be careful when handling the UV lamp.

There is a UV lamp inside of the duct. Be careful not to damage it when installing or removing chamber

attachments or the humidifying pan.

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Page 22

Setting the UV lamp ON period

The clauses below are applicable when the model is MCO-18AC(UV) or an optional UV system kit

MCO-18UVS3 is installed.

Follow the procedure below when changing the setting of the UV lamp ON period.

Basic operation sequence (Example: change of UV lamp ON period from 5 minutes to 3 minutes)

Description of operation Key operated Indication after operation

Press calibration key

for 5 seconds.

By pressing numerical value shift

key and digit shift key,

set the figure to F01.

3 Press enter key. ENT

By pressing numerical value shift

key and digit shift key,

set the figure to 003.

5 Press enter key. ENT

x The UV timer can be set from 0 to 30 min. The default setting is for 5 min. When the UV timer is set to zero, the UV lamp will not light. x If the outer door is opened while the UV lamp is lit, the lamp will turn OFF. Then, when the door is closed, the lamp will light for the preset period of time. x If the UV lamp ON time is set for longer than 5 minutes or if only the outer door is repeated opened and closed, condensation may occur in the chamber and may affect temperature distribution. It will also shorten the service life of the UV lamp. x When replacing the UV lamp, contact a Sanyo sales representative or agent.

CAL

The left digit in the digital

temperature indicator is blinked.

When pressed,

the figure of settable digit changes.

When pressed, the changeable digit

is shifted.

The current setting is displayed

in the digital CO

When pressed, the figure of

settable digit changes.

Set value is memorized and the display return to

normal display mode.

density indicator.

Pressing calibration key (CAL) for about 5 seconds leads the calibration mode. In the calibration mode,

the calibration of temperature and CO

performance. Never touch any other keys on the control panel in the event of pressing calibration key

(CAL) accidentally. After about 90 seconds, the incubator returns to chamber temperature display mode

automatically.

density is possible. Wrong key operation affects the basic

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Page 23

Lighting the UV lamp for 24 hours

The clauses below are applicable when the model is MCO-18AC(UV) or an optional UV system kit

MCO-18UVS3 is installed.

If the chamber has been contaminated by dirt or by spilling the medium, use the following procedure to decontaminate the chamber by lighting the UV lamp for 24 hours.

1. Remove all attachments from the chamber, including the trays, tray supports, side supports, fan cover, duct, fan, humidifying pan, and humidifying pan cover. Clean all the attachments in an autoclave or with disinfectant alcohol.

2. Clean and wipe off inside the chamber with disinfectant alcohol.

3. Set the CO

density to 0% and set the UV 24-hour lighting mode.

Description of operation Key operated Indication after operation

1 Press calibration key for 5 seconds. CAL

By pressing numerical value shift

key and digit shift key,

When pressed,

set the figure to F02.

3 Press enter key. ENT

4 By pressing numerical value shift

When pressed,

key, set the figure to 001.

5 Press enter key. ENT

The left digit in the temperature

indicator blinks.

When pressed, the figure of settable digit changes.

the changeable digit is shifted.

“000” is displayed

in the digital CO

density indicator.

the figure of settable digit changes.

Set value is memorized and the display returns to

normal display mode.

・This procedure must be preformed only with the outer door closed and the UV lamp turned OFF. ・The UV lamp will light continuously for 24 hours after this parameter is set. The setting is canceled if the

outer door is opened. Perform the above procedure to set the 24-hours lighting mode again when opening the outer door.

・The incubator keeps running with set temperature and CO

density during UV lighting.

・Install the attachments after completion of the 24-hour lighting mode.

Note: Set the upper limit alarm temperature to at least 10

C higher than the chamber set temperature

when using the 24-hour lighting mode of UV lamp. The 24-hour lighting mode may cause an automatic chamber temperature alarm because the temperature of the chamber will increase.

Pressing calibration key (CAL) for about 5 seconds leads the calibration mode. In the calibration mode,

the calibration of temperature and CO

density is possible. Wrong key operation affects the basic

performance. Never touch any other keys on the control panel in the event of pressing calibration key

(CAL) accidentally. After about 90 seconds, the incubator returns to chamber temperature display mode

automatically.

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Page 24

KEY LOCK FUNCTION

This incubator is provided with a key lock function. When the key lock is ON, change of temperature or

density setting through the key pad is not available.

Note: The key lock is set in OFF mode (L0) at the factory.

Display Mode Function

Key lock is OFF Enable to change of temperature and CO

Key lock is ON Disable to change of temperature or CO

setting

Procedure for key lock setting (change from key lock OFF to key lock ON)

Description of operation Key operated Indication after operation

1 ----

2 Press digit shift key for 5 seconds.

Press numerical value shift key

and scroll the figure to 1.

L0 is displayed in the temperature

When pressed, the figure of settable

4 Press enter key. ENT

The current chamber temperature and

density are displayed.

indicator.

digit changes.

The key lock is set to ON.

The current chamber temperature is displayed.

Note:

●The key lock function is available for temperature and CO

density setting.

● To cancel the key lock, set to L0 in the above procedure.

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Page 25

WATER LEVEL SENSOR

Water level sensor for the humidifying pan is used with the MCO-18AC(UV) or the MCO-18AC with an

optional UV system kit MCO-18UVS3 installed. The sensor is set in active position with respect to the

installation of the humidifying pan. Take care not to damage the sensor at the time of removal or

installation of the humidifying pan.

When the humidifying pan is removed (side view)

Sensor

Humidifying pan

(MCO-18AC(UV) or MCO-18AC with MCO-18UVS3)

When the humidifying pan is installed (side view)

Sensor

Humidifying pan

Note: At the installation, check the humidifying pan is located far depth and the sensor is down.

Note:

• Lift the sensor before installing the humidifying pan if the sensor is in lower position after maintenance.

• At the time of installation of humidifying pan, check that the pan is set properly and sensor is down in

the pan. The water level alarm lamp (RH PAN) blinks if the sensor is not down completely. In this case,

set the pan again in adequate location.

• The sensor detects the water level every 30 minutes. In addition, detection is executed after operation of

the outer door. It takes several seconds to detect the water level. Therefore, the water level alarm

lamp may blink a few times after the outer door is closed with the humidifying pan filled sufficiently.

CAUTION

The foreign particles on the water surface adhere to the water level sensor and its fittings by the capillary

action because the sensor is always in the water. The adhered foreign particles degrade the sensor

performance and the water level alarm lamp may blink with the enough water in the humidifying pan.

At the cleaning, take care not transmit the excessive force to the sensor lead wire.

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Page 26

ALARMS, SAFETY, AND SELF-DIAGNOSIS

The incubator supports the following alarms, safety functions, and self-diagnostic functions.

Alarms, safety and self-diagnosis for culture operations

Alarm or safety

function

Upper limit temperature alarm

Automatic set temperature alarm

Automatic set CO density alarm

Door check The outer door is open. Door lamp lights -----

Auto-return

lock When the key lock is “ON”. ----- ----- The setting is disabled.

gas cylinder

empty

Gas line changeover

Chamber temperature sensor abnormality

Ambient temperature sensor abnormality

sensor

abnormality

Main heater abnormality

Bottom heater abnormality

Door heater abnormality

Disconnection of sensor for each heater

Low humidifying Wate

UV lamp failure* When the UV lamp is burned out.

Recommendation of new UV lam

If the chamber temperature exceeds the upper limit alarm temperature set value.

If the chamber temperature deviates from the set temperature by ±1 more. If the chamber CO

from the set value b

When there is no key pressing in each settin

If the CO2 density does not increase when the gas valve is opened.

When the gas supply line is switched. (only when MCO-21GC is installed)

If the temperature sensor is disconnected.

If the temperature sensor is short circuited.

If the ambient temperature sensor is disconnected.

If the ambient temperature sensor is short circuited.

If the output voltage of the CO2 sensor is abnormal.

If the upper limit alarm temperature alarm operates, or if the main heater is open circuit, or the main heater relay short circuit.

If the bottom heater goes open circuit, or the bottom heater relay short circuit.

If the door heater goes open circuit, or the door heater relay short circuit.

If the relay of main heater, bottom heater or sensor box heater goes open circuit.

If the water in the humidifying pan is about 0.8 liter.

The accumulated ON time is over about 1,000 hrs.

*MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

Conditions Indication Buzzer Safety operation

Over heat lamp lights.

density deviates

±1% or more.

mode for 90 seconds.

C or

E12 or E16 and chamber temperature are displayed alternatel

All digits on the temperature indicator blink.

All digits on the digital CO density indicator blink.

Normal display mode. -----

E01 is displayed alternatel with the temperature on the digital temperature indicator. E01 is displayed alternatel with the temperature on the digital temperature indicator. CO2 gas supply line indicatorof empty blinks. E05 is displayed alternatel with the temperature on the digital temperature indicator. E06 is displayed alternatel with the temperature on the digital temperature indicator. E09 is displayed alternatel with the temperature on the digital temperature indicator. E10 is displayed alternatel with the temperature on the digital temperature indicator. E11 is displayed alternatel with the temperature on the digital temperature indicator. E12 is displayed alternatel with the temperature on the digital temperature indicator. E13 is displayed alternatel with the temperature on the digital temperature indicator. E14 is displayed alternatel with the temperature on the digital temperature indicator. E16 is displayed alternatel with the temperature on the digital temperature indicator. Water level alarm lamp(RH PAN)blinks. E18 is displayed alternatel with the temperature on the digital temperature indicator. UV indicator blinks when UV lamp is OFF.

Continuous tone

Intermittent tone with 15 minutes delay.

Intermittent tone with

15 minutes delay.

Intermittent tone Remote alarm

Intermittent tone

Intermittent tone Remote alarm

Intermittent tone

Intermittent tone Remote alarm

----- -----

Intermittent tone Remote alarm

----- -----

Heater OFF Remote alarm

Remote alarm with 15 minutes delay

Remote alarm with 15 minutes dela Fan motor OFF CO

valve is closed

UV lamp OFF* (60 seconds after) Heater OFF The setting mode is canceled.

Gas supply line is altered. Remote alarm

Heater OFF Remote alarm

valve is closed

Remote alarm

• The alarm can be canceled by pressing the alarm buzzer stop key (BUZZER), but the remote alarm cannot be stopped. And the upper limit temperature alarm cannot be silenced with the alarm buzzer stop key (BUZZER).

• E01 is cleared automatically when the gas is connected correctly and the buzzer is silenced with the alarm buzzer stop key (BUZZER). When MCO-21GC is installed, press the alarm buzzer stop key (BUZZER) to silence the alarm after changeover of gas supply line.

• If one of E05 to E18 (except for E12, E13, and E14) is displayed, contact a Sanyo sales representative or agent.

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Page 27

SETTING OF ALARM RESUME TIME

The alarm buzzer is silenced by pressing alarm buzzer stop key (BUZZER) on the control panel during

alarm condition (The remote alarm is not stopped).

The buzzer will be activated again after certain suspension if the alarm condition is continued (The remote alarm continues). The suspension time can be set by following the procedure shown in the table

below.

The example in the table is based on the assumption that the desired duration is 20 minutes.

Note: The duration is set in 30 minutes at the factory.

Table Changing procedure for alarm resume time (Ex: change from 30 minutes to 20 minutes)

Description of operation Key operated Indication after operation

1 ----

Press calibration key

for 5 seconds.

Set the figure to F25 with the numerical value shift key

and digit shift key.

CAL The left digit blinks.

The current chamber temperature is displayed.

When pressed,

the figure of settable digit changes.

The settable digit is shifted.

4 Press enter key. ENT

Set the figure to 020 with the

numerical value shift key.

6 Press enter key. ENT

• The settable alarm resume time are 0, 10, 20, 30, 40, 50, or 60 minutes (The setting is 000, 010, 020,

030, 040, 050 or 060 respectively). The buzzer would not reset if the reset time is set in 000.

• It is recommended to set the alarm resume time when the incubator is not under alarm condition. The

setting during alarm condition is effective on the next alarm condition.

• The setting cannot be changed during power failure.

• The remote alarm during power failure cannot be stopped.

• The set mode returns to the temperature display mode automatically when 90 seconds has passed

without any key operation. In this case, any setting before pressing enter key is not memorized.

Pressing calibration key (CAL) for about 5 seconds leads the calibration mode. In the calibration mode,

the calibration of temperature and CO

performance. Never touch any other keys on the control panel in the event of pressing calibration key

(CAL) accidentally. After about 90 seconds, the incubator returns to chamber temperature display mode

automatically.

density is possible. Wrong key operation affects the basic

The current setting is displayed.

The middle digit blinks.

When pressed,

the figure of settable digit changes.

The setting is memorized and the current chamber temperature is displayed.

Operation after power failure

The set value is memorized by nonvolatile memory. Accordingly, the incubator resumes the operation

with setting before power failure.

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Page 28

ROUTINE MAINTENANCE

Cleaning the chamber and inner attachments

When the chamber of the incubator is contaminated, the chamber and internal attachments should be

cleaned and sterilized as follows.

WARNING

Before performing any repairs or maintenance, turn OFF the power switch and unplug the incubator.

Failure to do say may result in electric shock or injury.

Be careful not to inhale chemicals, vapors or aerosols when cleaning the incubator. Doing so may

be harmful to health.

CAUTION

Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may

result in cuts or abrasions from sharp edges or corners.

Note:

Be careful not to damage the water level sensor or the UV lamp in the chamber duct (if the

MCO-18AC(UV) or an optional UV system kit MCO-18UVS3 is installed).

Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause

discoloration, corrosion, or rusting.

1. Close the valve of the CO

2. Open the outer door. Remove 2 pins fixing the inner door hinge and remove the inner door.

3. Pull out the all trays. (See Fig. 1.)

4. Lift the front of the tray supports and pull them out. (See Fig. 2.)

5. (MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed)

Lift the humidifying pan cover off from the pins on the rear side. (See Fig. 3.)

gas cylinder and turn off the power to the incubator.

Tray support

Humidifying pan cover

Fig. 1 Fig.2 Fig.3

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Page 29

6. Pull out the humidifying pan. (See Fig. 4.)

7. Loosen the two screws securing the fan cover and take off the fan cover. (See Fig. 5.)

8. Lift the duct and remove it from the pins on the rear side. (See Fig. 6.)

Fan cover

Humidifying pan

Fig.4 Fig.5 Fig.6

9. Remove the fan by pulling out the central spring and then by pulling out the fan. (See Fig. 7.)

10. Remove the screw securing the clamp for the side support and remove the clamp. (See Fig. 8.)

11. Lift the side support off of the pins. (See Fig. 9.)

Fan

Clamp

Side

support

Duct

Fig.7 Fig.8 Fig.9

12. Clean all the attachments with a diluted neutral detergent, and then rinse them thoroughly with

distilled water.

13. Wipe the trays, the inner attachments such as the fan, and the chamber sides with sterilizing alcohol.

Be careful not to leave any residual alcohol.

14. Wipe the inside wall of the chamber with a gauze containing alcohol for sterilization and then wipe off

with a dry gauze.

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Page 30

15. (MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed)

Wipe the water level sensor with sterilizing alcohol. Be careful not to leave any residual alcohol. When

cleaning the sensor, take care not to apply excessive force to the lead wires.

16. To reinstall all the attachments, perform the procedure in reverse order from step 11.

17. Fill the humidifying pan with sterile distilled water.

Note:

• When installing the fan, insert it securely on the motor shaft (See Fig.10). Lightly turn the fan manually

to make sure that it does not strike the rear panel. Improper insertion may cause poor performance.

• As shown in Fig. 11, set the tray with only the front edge bent down. If the tray is set in the wrong

direction, it may not be level and may become unstable.

Fan

Spring

①②

Fig. 10

Bent down

Fig. 11

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Page 31

Filling the humidifying pan

Use the following procedure to fill the humidifying pan or to replace the water.

1. (MCO-18AC(UV) or when an optional UV system

kit MCO-18UVS3 is installed)Lift the humidifying pan

cover. After that pull the humidifying pan forward.

(See Fig. 1.)

2. Dispose of the remaining water in the humidifying

pan and clean the humidifying pan with a neutral

dishwashing detergent. Then rinse it thoroughly with

distilled water and wipe it with sterilizing alcohol.

3. Wipe all moisture from the bottom of the chamber.

4. Return the humidifying pan to the chamber and

add sterile distilled water (approx. 1.5 L, preheated

to 37qC). (See Fig. 2.)

Note:

Preheat to 37qC the water to be added to the

humidifying pan. Adding low-temperature water will

lower the temperature and humidity in the chamber.

Fig. 1

Fig. 2

5. Set the humidifying pan with the inner side flush against the back, and close the inner and outer doors.

(MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed)

Set the humidifying pan with the inner side flush against the back, and replace the humidifying cover

(Refer to page 24). Close the inner and outer doors, and confirm that water level alarm lamp (RH PAN)

on the control panel is off.

Note:

(MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed)

If the water level alarm lamp(RH PAN) blinks, use the above procedure to replenish the water.

CAUTION

(MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed)

When refilling the water in the humidifying pan, always wipe off any dirt from the water level sensor

with sterilizing alcohol. While doing that, be careful not to apply excessive force to the sensor lead

wire.

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Page 32

CALIBRATION

Temperature calibration

1. Press the calibration key (CAL) for approximately 5 seconds again to enter the temperature calibration

input mode.

2. The left digit of the digital temperature indicator blinks, and the digital CO

3. Set the present correct temperature with the digit shift key ( ) and numerical value shift key ( ) ,

then press the enter key (ENT).

4. The incubator automatically reverts to the display mode.

[Example]

If the displayed chamber temperature is 37.0

1. Press the calibration key (CAL) for about 5 seconds.

2. The “3” on the digital temperature indicator blinks, and the digital CO

3. Adjust the set value to the actual value of 36.8

key ( ), then press enter key (ENT).

4. The incubator automatically reverts to the display mode.

density indicator goes out.

C (set value) and the actual temperature is 36.8oC.

density indicator goes out.

C with the digit shift key ( ) and numerical value shift

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Page 33

CALIBRATION

CO2 calibration

Span setting

Span setting should be done under stable condition of temperature, humidity, and CO

1. Press the calibration key (CAL) once to enter the CO

2. Confirm blinking the decimal point in the digital CO

3. Open the outer door and remove the sample air outlet cap on the inner door.

4. Measure the CO

5. Press the calibration key (CAL) for about 5 seconds again to enter the temperature calibration input

mode.

6. The left digit on the digital temperature indicator blinks, and the digital CO

7. Press the calibration key (CAL) once again to enter the CO

8. The left digit on the digital CO

9. Set the present correct CO

then press the enter key (ENT).

10. Replace the sample air outlet cap.

11. The incubator automatically reverts to the display mode.

Note:

This calibration is available when the setting of CO

density in the chamber through the sample air outlet on the inner door.

density.

calibration pre-conditioning mode.

density indicator.

density indicator goes out.

calibration input mode.

density indicator blinks, and the temperature indicator goes out.

density with the digit shift key ( ) and numerical value shift key ( ),

density is 2% or more.

[Example]

For an internal CO

density of 5.0% (setting) and a measured value of 4.5%.

1. Press the calibration key (CAL) once.

2. Press the calibration key (CAL) for about 5 seconds again.

3. The “3” on the digital temperature indicator blinks, and the digital CO

density indicator goes out.

4. Press the calibration key (CAL) once again.

5. The “0” on the digital CO

left digit on the digital CO

6. Set the present correct CO

density indicator blinks, and the digital temperature indicator goes out. The

density indicator blinks, and the temperature indicator goes out.

density (4.5%) with the digit shift key ( ) and numerical value shift key

( ), then press the enter key (ENT).

7. The incubator automatically reverts to the display mode.

Note:

It is important to accurately measure the temperature inside the incubator when calibrating the

temperature. Particularly, the temperature gauge used must have an accuracy of JIS 0.5 Class or better.

The temperature must be measured at several points. The temperature setting must not change by more than ±1.0

C during calibration. If these values are

exceeded, an error buzzer will sound, the input data will be ignored, and the incubator will return to the

display mode. If it is necessary to change the temperature by more than 1.0

C, perform calibration in

several stages over a period of time.

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Page 34

TROUBLESHOOTING

If the incubator does not seem to be working properly, check the following items before calling for service.

Malfunction Check/Remedy

The incubator does not operate at all.

The key operation is disable • The key lock function is set in ON mode.

If the alarm function and the buzzer operates

If the chamber temperature is not equal to the set temperature

If the CO

gas density does

not coincide with the set value

If the chamber humidity does not rise

If the CO

consumption is

too much

If normal cultivation cannot be done and chamber gas density is suspect

If it takes much time to recover the CO

gas is not being

gas density

injected.

• The incubator is not plugged correctly into a power outlet.

• The circuit breaker at the power source is active or a power

failure has occurred.

[At the beginning of operation]

• The chamber temperature is not equal to the set value.

• The chamber CO

a. The secondary pressure of the pressure regulator is not equal to the set value (0.03MPaG, 0.3kgf/cm b. The tube is not connected securely between the pressure regulator and the incubator.

[During operation]

• The upper limit alarm temperature is not set at least 1 than the set chamber temperature.

• The set temperature value was changed, or the door was left open for a long period. Or a low temperature load was placed inside the incubator. In this case, if the incubator is left as it is, the alarm will eventually clear itself.

• The gas tube has slipped off or the gas leaks.

• The set value of the gas density was changed.

• The gas cylinder is empty. Check the primary pressure of the

cylinder once a week. (The primary pressure of less than

3.8MPaG (38kgf/cm Replace the cylinder soon.)

• The ambient temperature must always be at least 5 the set temperature.

• The outer door was closed while the inner door was left open.

• The secondary pressure is not set to 0.03MPaG (0.3kgf/cm

4.3psiG).

• The gas tube is clogged or chinked.

• The humidifying pan is not filled with sterile distilled water.

(Always use sterile distilled water.)

• The humidifying pan is not filled with sterile distilled water. (Always use sterile distilled water.)

• The door is opened frequently.

• There is any gas leakage at the connection or pin hole on the

tube. It is recommended to replace the tube once a year.

• The gasket of the inner door is not completely sealed.

• The access port at the upper left corner is opened.

• The environment around the incubator is not normal. The

source of the contaminated gas is nearby.

• The incubator is installed in an enclosed space.

• HEPA filter is provided in the gas piping. If it takes much time

to recover the gas density even though the gas pressure is normal, it may be that dust on the HEPA filter prevents the gas flow. Contact a Sanyo sales representative or agent.

• The CO

method. CO concentration in the chamber approaches the set value. Injections may be stopped for periods of approximately 15 seconds, but that is not an error.

density is not equal to the set value.

G, 4.3psiG).

C higher

G) means a little gas in the cylinder.

C less than

control method for the incubator is the ON-OFF

gas is intermittently injected as the gas

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Page 35

Note: If the problem still has not been solved after trying the above checks and countermeasures, or for

any problems not covered here, contact a Sanyo sales representative or agent.

DISPOSING OF THE CO2 INCUBATOR

When disposing of the CO2 incubator, contact a Sanyo sales representative or agent.

WARNING

The incubator must be dismantled and disposed of by qualified personnel only. If the incubator is

left where outsiders enter, it may result unexpected accident (for example, children to become locked

inside).

Before disposing a CO

extent possible by the user.

incubator with biohazardous danger, decontaminate the CO2 incubator to the

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Page 36

Note: This symbol mark and recycle system are applied only to EU countries and not applied to the countries in the other area of the world.

Waste Electrical and Electronic Equipment (WEEE) Directive-2002/96/EC

(English)

Your SANYO product is designed and manufactured with high quality materials and components which can be recycled and reused.

This symbol means that electrical and electronic equipment, at their end-of-life, should be disposed of separately from your household waste.

Please dispose of this equipment at your local community waste collection/recycling centre.

In the European Union there are separate collection systems for used electrical and electronic products.

Please help us to conserve the environment we live in!

(German)

Ihr SANYO Produkt wurde entworfen und hergestellt mit qualitativ hochwertigen Materialien und Komponenten, die recycelt und wiederverwendet werden können.

Dieses Symbol bedeutet, daß elektrische und elektronische Geräte am Ende ihrer Nutzungsdauer von Hausmüll getrennt entsorgt werden sollen.

Bitte entsorgen Sie dieses Gerät bei Ihrer örtlichen kommunalen Sammelstelle oder im Recycling Centre.

In der Europäischen Union gibt es unterschiedliche Sammelsysteme für Elektrik- und Elektronikgeräte.

Helfen Sie uns bitte, die Umwelt zu erhalten, in der wir leben!

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Page 37

(French)

Votre produit Sanyo est conçu et fabriqué avec des matèriels et des composants de qualité supérieure qui peuvent être recyclés et réutilisés.

Ce symbole signifie que les équipements électriques et électroniques en fin de vie doivent être éliminés séparément des ordures ménagères.

Nous vous prions donc de confier cet équipement à votre centre local de collecte/recyclage. Dans l’Union Européenne, il existe des systèmes sélectifs de collecte pour les produits électriques et électroniques usagés.

Aidez-nous à conserver l’environnement dans lequel nous vivons !

Les machines ou appareils électriques et électroniques contiennent fréquemment des matières qui, si elles sont traitées ou éliminées de manière inappropriée, peuvent s’avérer potentiellement dangereuses pour la santé humaine et pour l’environnement. Cependant, ces matières sont nécessaires au bon fonctionnement de votre appareil ou de votre machine. Pour cette raison, il vous est demandé de ne pas vous débarrasser de votre appareil ou machine usagé avec vos ordures ménagères.

(Spanish)

Los productos SANYO están diseñados y fabricados con materiales y componentes de alta calidad, que pueden ser reciclados y reutilizados.

Este símbolo significa que el equipo eléctrico y electrónico, al final de su ciclo de vida, no se debe desechar con el resto de residuos domésticos.

Por favor, deposite su viejo “televisor” en el punto de recogida de residuos o contacte con su administración local.

En la Unión Europea existen sistemas de recogida específicos para residuos de aparatos eléctricos y electrónicos.

Por favor, ayúdenos a conservar el medio ambiente!

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Page 38

(Portuguese)

O seu produto SANYO foi concebido e produzido com materiais e componentes de alta qualidade que podem ser reciclados e reutilizados.

Este símbolo significa que o equipamento eléctrico e electrónico no final da sua vida útil deverá ser descartado separadamente do seu lixo doméstico.

Por favor, entregue este equipamento no seu ponto local de recolha/reciclagem.

Na União Europeia existem sistemas de recolha separados para produtos eléctricos e electrónicos usados.

Por favor, ajude-nos a conservar o ambiente em que vivemos!

(Italian)

Il vostro prodotto SANYO è stato costruito da materiali e componenti di alta qualità, che sono riutilizzabili o riciclabili.

Prodotti elettrici ed elettronici portando questo simbolo alla fine dell’uso devono essere smaltiti separatamente dai rifiuti casalinghi.

Vi preghiamo di smaltire questo apparecchio al deposito comunale. Nell’Unione Europea esistono sistemi di raccolta differenziata per prodotti elettrici ed elettronici.

Aiutateci a conservare l’ambiente in cui viviamo!

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Page 39

(Dutch)

Sanyo producten zijn ontwikkeld en gefabriceerd uit eerste kwaliteit materialen, de onderdelen kunnen worden gerecycled en weer worden gebruikt.

Het symbool betekent dat de elektrische en elektronische onderdelen wanneer deze vernietigd gaan worden , dit separaat gebeurt van het normale huisafval.

Zorg ervoor dat het verwijderen van de apparatuur bij de lokaal erkende instanties gaat gebeuren. In de Europese Unie wordt de gebruikte elektrische en elektronische apparatuur bij de daarvoor wettelijke instanties aangeboden.

Alstublieft help allen mee om het milieu te beschermen.

(Swedish)

Din SANYO produkt är designad och tillverkad av material och komponenter med hög kvalitet som kan återvinnas och återanvändas.

Denna symbol betyder att elektriska och elektroniska produkter, efter slutanvändande, skall sorteras och lämnas separat från Ditt hushållsavfall.

Vänligen, lämna denna produkt hos Din lokala mottagningstation för avfall/återvinningsstation.

Inom den Europeiska Unionen finns det separata återvinningssystem för begagnade elektriska och elektroniska produkter.

Vänligen, hjälp oss att bevara miljön vi lever i!

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Page 40

AUTOMATIC CO2 CYLINDER CHANGEOVER

An automatic CO

system switches the gas supply line when one CO

cylinder changeover system (MCO-21GC) is available as an optional accessory. This

gas cylinder is empty.

Note: The installation of the MCO-21GC must be performed only by qualified service personnel.

Install the MCO-21GC and then perform the following procedure

1. Connect the CO

gas pipes to connecting ports for CO2 gas pipes A and B. Connecting ports for CO

gas pipes are located on the left back of the incubator. (Refer to page 8).

Note: Only port A can be used when the MCO-21GC is not installed. There will be 2 ports, ports A and B, after the MCO-21GC is installed.

2. Connect a CO

for information on connecting the CO

to the CO

gas pipe connected to port B.)

gas cylinder equipped with a gas pressure regulator to each gas pipe. Refer to page 14

gas cylinders. (“Gas cylinder A” is the CO2 gas cylinder connected

gas pipe connected to port A, and “gas cylinder B” is the CO2 gas cylinder connected to the

3. Open the valve of both gas cylinders.

4. Check that the CO

gas supply line indicator on the control panel is lit by pressing the CO2 gas supply

line switching key.

5. Select a CO

6. The MCO-21GC detects that there is no more CO

chamber does not increase for a while even after opening the CO

gas supply line (A or B).

gas in a cylinder when the CO2density in the

gas valve in the incubator. It switches

the gas supply line when it detects that there is no more gas. At that time, the following indications of

switching the gas cylinder are provided.

gas supply line indicator

Digital temperature indicator

Buzzer

Empty line The other line

E01 is displayed alternately with the

temperature on the digital

Intermittent tone Blinks Lights

temperature indicator.

●To silence the buzzer, press the alarm buzzer stop key (BUZZER). The alarm condition is cleared and

normal operation continues.

7. When gas cylinder A is switched to B, remove gas cylinder A and replace it with new one.

Note: Be careful when handling the empty CO

gas cylinder. Some gas may still be left in the cylinder.

8. When gas cylinder B becomes empty, the line is switched to gas cylinder A again.

The MCO-21GC detects that there is no more CO

does not increase for a while even after opening the CO

gas in a cylinder when the CO2density in the chamber

gas valve in the incubator. The supply line may

also be switched for other reasons even though there is gas left in the cylinder: blocking or restricting of

gas tube, reduction of CO

gas pressure, or improper opening of CO2 gas cylinder. Always check the gas

quantity in the cylinder before disconnecting it.

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Page 41

STACKING INCUBATORS

Use the following procedure to stack incubators. This work is potentially dangerous, so contact a Sanyo

sales representative or agent.

CAUTION

x Select a floor that is strong enough to support the stacked incubators.

x Never stack three or more incubators. Doing so is dangerous.

1. Take out two stacking plates A, two stacking plates B, four protective stickers, and four screws from

accessory bag.

Note:

When stacking two incubators, use two stacking plates (A and B) for each one. Only one pair of

stacking plates is provided per incubator, so take out the stacking plates (A and B) from both incubators.

Similarly only 2 protective stickers are provided per incubator

2. Unplug both of the incubators that are to be stacked.

3. Remove the caps at the two places in the front of the top surface of the incubator that is to be on the

bottom, and attach the protective stickers to the four corners on the top surface of the incubator that is to

be on the bottom. (See Fig. A. on the page 41)

4. Secure stacking plates A with the screws that are provided.

5. Remove the front panel (4 screws) from the incubator that is to be on the top.

6. Place the upper incubator on top of the lower one, being careful not to pinch your fingers.

7. Make the lower incubator level by adjusting the leveling feet, and then level the upper incubator in the

same way.

8. Secure stacking plates A on the top surface of lower incubator to the upper one with the accessory

screws.

9. Remove from the back of the lower incubator the two hooks (4 screws) and remove from the upper

incubator the 2 screws on the lower rear panel.

10. Use the 6 screws that were removed in step 9 to secure stacking plates B to the rear panels of the

upper and lower incubators.

11. Replace the front panel (4 screws) to the upper incubator.

12. Use the hooks on the back of the upper incubator to prevent the incubators from falling over.

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Page 42

Front panel

Front side

Stacking plate A

Stacking plate B

Protective sticker

Hook

Fig.A

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Page 43

SPECIFICATIONS

Name CO2 incubator

Model MCO-18AC MCO-18AC(UV)

External dimensions W620 x D710 x H900 (mm)

Internal dimensions W490 x D523x H665 (mm)

Interior volume 170 L

Exterior Painted steel

Interior Stainless steel containing copper

Outer door Painted steel Inner door Tempered glass

Tray 3 trays made of stainless steel containing copper

Access port Inner diameter; 30 mm, On the back side

Insulation Rigid polyurethane foamed-in place

Heating system DHA system (heater jacket + air jacket system)

Heater 282 W

Humidifying system Natural evaporation with humidifying pan

Temperature controller PID control system

Temperature display Digital display

controller ON-OFF control system/TC sensor

density display Digital display

Air circulation Fan assisted

Air filter

UV lamp

Water level sensor

Alarm

Automatic set temperature alarm, Automatic set CO

Upper limit temperature alarm, various gas/sensor/heater alarms Remote alarm contact Allowable contact capacity: DC 30 V, 2 A

inlet connection 4 to 6 mm diameter tube can be connected

inlet pressure 0.03MPaG (0.3kgf/cm2G, 4.3psiG)

Accessories 3 trays, 3 sets of tray support, 1 gas tube, 1 humidifying pan,

Stacking plate A and B, 2 protective stickers, 2 tube bands

Weight 92 kg 93 kg

Optional accessory

Note: Design or specifications will be subject to change without notice.

UV system kit (MCO-18UVS3) Standard equipment

UV replacement kit (MCO-20UV), 4-20mA Interface(MCO-420MA, USA only)

Extra tray (MCO-47ST) including 2 tray supports, Half tray (MCO-25ST)

Automatic CO

RS232C/RS485 Interface (MTR-480), Ethernet (LAN) Interface (MTR-L03)

W450 x D450 x H12 (mm), Maximum load; 7 kg/tray

0.3μm, Efficiency; 99.97% or more

----- 4W X 1 (ozone-free emission)

-----

----Low humidifying water alarm

Optical type

UV lamp failure alarm

density alarm

Gas pressure regulator (MCO-100L)

cylinder changeover system (MCO-21GC), Roller base (MCO-18RB)

SANYO Data acquisition software (MTR-5000)

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Page 44

PERFORMANCE

Temperature control range

Temperature distribution

Temperature variation

control range

variation

Ambient temperature +5

± 0.25

C (ambient temperature; 25oC, setting; 37oC, CO2 5%, no load)

± 0.1

C (ambient temperature; 25oC, setting; 37oC, CO2 5%, no load)

± 0.15% (ambient temperature; 25

Chamber humidity

Heat emission

C to 35oC, Humidity: equal or less than 80% R.H.

Applicable environment condition

Temperature: 5

(The designed performance may not be obtained when

the ambient temperature is equal or less than 15

Noise level

Power consumption

Rated voltage, frequency Amperage

Max. 300 W Max. 310 W

110 to 120 V, 60 Hz 220 V, 60 Hz 220 to 240 V, 50 Hz

Max. 2.6 A Max. 1.4 A Max. 1.4 A

Note: The unit with CE mark complies with EC directives.

It is based on our measuring method.

C to 50oC (ambient temperature; 5oC to 35oC)

0 to 20%

C, setting; 37oC, CO2 5%, no load)

95 ± 5%RH

Max. 1116 kJ/h

24 dB (A scale)

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Page 45

CAUTION

Please fill in this form before servicing.

Hand over this form to the service engineer to keep for his and your safety.

Safety check sheet

1. Incubator contents : Risk of infection: Risk of toxicity: Risk from radioactive sources:

(List all potentially hazardous materials that have been stored in this unit.) Notes :

Contamination of the unit

Unit interior No contamination Decontaminated Contaminated Others:

□Yes □No

□Yes □No □Yes □No

□Yes □No

□Yes □No □Yes □No

□Yes □No

3. Instructions for safe repair/maintenance of the unit a) The unit is safe to work on b) There is some danger (see below) Procedure to be adhered to in order to reduce safety risk indicated in b) below.

Date : Signature :

Address, Division :

Telephone :

Product name :

incubator

Please decontaminate the unit yourself before calling the service engineer.

Model :

MCO-18AC

MCO-18AC(UV)

- 93 -

□Yes □No

□Yes □No □Yes □No

□Yes □No

Serial number : Date of Installation :

Page 46

SANYO Electric Co., Ltd

Printed in Japan

Sanyo MCO-18AC9UV) Installation Manual

Specifications and Main Features

Frequently Asked Questions

User Manual