Sanyo MCO-18AC9UV) Installation Manual

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INSTRUCTION MANUAL

CO2 Incubator

MCO-18AC(UV MCO-18AC

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CONTENTS

INTRODUCTION P. 2

PRECAUTIONS FOR SAFE OPERATION P. 3

LABELS ON THE INCUBATORS P. 6

ENVIRONMENTAL CONDITIONS P. 7

INCUBATOR COMPONENTS P. 8

Control panel and keypad P. 10

Remote alarm terminal P. 11

INSTALLATION SITE P. 12

INSTALLATION P. 13

Connection of CO

PREVENTING CONTAMINATION P. 15

PRECAUTIONS FOR CULTURES P. 16

START-UP OF INCUBATOR P. 17

SETTING THE CHAMBER TEMPERATURE AND CO

UV LAMP

Precautions when using the UV lamp P. 20

Setting the UV lamp ON period P. 21

Lighting the UV lamp for 24 hours P. 22

KEY LOCK FUNCTION P. 23

WATER LEVEL SENSOR

ALARMS, SAFETY, AND SELF-DIAGNOSIS P. 25

SETTING OF ALARM RESUME TIME P. 26

Operation after power failure P. 26

ROUTINE MAINTENANCE

Cleaning the chamber and inner attachments P. 27

Filling the humidifying pan P. 30

CALIBRATION

Temperature calibration P. 31

calibration P. 32

TROUBLESHOOTING P. 33

DISPOSING OF THE CO

AUTOMATIC CO

STACKING INCUBATORS P. 40

SPECIFICATIONS P. 42

PERFORMANCE P. 43

SAFETY CHECK SHEET P. 44

gas cylinder P. 14

(MCO-18AC(UV) or MCO-18AC with MCO-18UVS3)

INCUBATOR P. 34

CYLINDER CHANGEOVER P. 39

DENSITY P. 18

P. 19

P. 24

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INTRODUCTION

■ Read this manual carefully before using the appliance and follow the instructions for safety operation.

■ Sanyo never guarantee any safety if the appliance is used for any objects other than intended use or

used by any procedures other than those mentioned in this manual.

■ Keep this manual in an adequate place to refer to it as necessary.

■ The contents of the manual will be subjected to change without notice due to the improvement of

performance or functions.

■ Contact Sanyo sales representative or agent if any page of the manual is lost or page order is incorrect.

■ Contact Sanyo sales representative or agent if any point in this manual is unclear or if there are any

inaccuracies.

■ No part of this manual may be reproduced in any form without the expressed written permission of

Sanyo.

CAUTION

SANYO guarantees the product under certain warranty conditions. SANYO in no way shall be responsible for any loss of content or damage of content.

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PRECAUTIONS FOR SAFE OPERATION

It is imperative that the user complies with this manual as it contains important safety advice.

Items and procedures are described so that you can use this unit correctly and safely. If the precautions advised are followed, this will prevent possible injury to the user and any other person.

Precautions are illustrated in the following way:

WARNING

Failure to observe WARNING signs could result in a hazard to personnel possibly resulting in serious injury or death.

CAUTION

Failure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property.

Symbol shows;

this symbol means caution.

this symbol means an action is prohibited.

this symbol means an instruction must be followed.

Be sure to keep this manual in a place accessible to users of this unit.

< Label on the unit >

This mark is labeled on the cover in which the electrical components of high voltage are enclosed to prevent the electric shock. The cover should be removed by a qualified engineer or a service personnel only.

WARNING

As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity of the equipment. It is important that you assess the work site to ensure there is suitable and sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a safe environment must be considered. These may include atmosphere monitoring and warning devices.

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WARNING

Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to

rain water.

Only qualified engineers or service personnel should install the unit. The installation by

unqualified personnel may cause electric shock or fire.

Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from

turning over. If the floor is not strong enough or the installation site is not adequate, this may result

in injury from the unit falling or tipping over.

Never install the unit in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

Never install the unit in a flammable or volatile location. This may cause explosion or fire.

Never install the unit where acid or corrosive gases are present as current leakage or electric

shock may result due to corrosion.

Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not

grounded, it will be necessary to install a ground by qualified engineers.

Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

Connect the unit to a power source as indicated on the rating label attached to the unit. Use

of any other voltage or frequency other than that on the rating label may cause fire or electric shock.

Never store volatile or flammable substances in this unit if the container cannot be sealed. These

may cause explosion or fire.

Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.

This may cause electric shock or injury by accidental contact with moving parts.

Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use

may cause bad effect on your health or environment.

Turn off the power switch (if provided) and disconnect the power supply to the unit prior to

any repair or maintenance of the unit in order to prevent electric shock or injury.

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet

hand. This may cause electric shock.

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WARNING

Ensure you do not inhale or consume medication or aerosols from around the unit at the time of

maintenance. These may be harmful to your health.

Never splash water directly onto the unit as this may cause electric shock or short circuit.

Never put containers with liquid on the unit as this may cause electric shock or short circuit when

the liquid is spilled.

Never bind, process, or step on the power supply cord, or never damage or break the power

supply plug. A broken supply cord or plug may cause fire or electric shock.

Do not use the supply cord if its plug is loose. Such supply cord may cause fire or electric shock.

Never disassemble, repair, or modify the unit yourself. Any such work carried out by an

unauthorized person may result in fire, or electric shock or injury due to a malfunction.

Disconnect the power supply plug if there is something wrong with the unit. Continued

abnormal operation may cause electric shock or fire.

When removing the plug from the power supply outlet, grip the power supply plug, not the cord.

Pulling the cord may result in electric shock or fire by short circuit.

Disconnect the power supply plug before moving the unit. Take care not to damage the power

cord. A damaged cord may cause electric shock or fire.

Disconnect the power plug when the unit is not used for long periods. Keeping the connection

may cause electric shock, current leakage, or fire due to the deterioration of insulation.

If the unit is to be stored unused in an unsupervised area for an extended period, ensure that

children do not have access and that doors cannot be closed completely.

The disposal of the unit should be accomplished by appropriate personnel. Remove doors to

prevent accidents such as suffocation.

Do not put the packing plastic bag within reach of children as suffocation may result.

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CAUTION

Use a dedicated power source (a dedicated circuit with a breaker) as indicated on the rating label

attached to the unit. A branched circuit may cause fire resulting from abnormal heating.

Connect the power supply plug to the power source firmly after removing the dust on the plug.

A dusty plug or improper insertion may cause a heat or ignition.

Never store corrosive substances such as acid or alkali in this unit if the container cannot be

sealed. These may cause corrosion of inner components or electric parts.

Check the setting when starting up of operation after power failure or turning off of power

switch. The stored items may be damaged due to the change of setting.

Be careful not to tip over the unit during movement to prevent damage or injury.

Prepare a safety check sheet when you request any repair or maintenance for the safety of service

personnel.

LABELS ON THE INCUBATORS

Warning and caution labels are attached to the incubator. The following table describes the labels.

This label is attached to covers that access high-voltage electrical components to prevent electric shock. Only a qualified engineer or service personnel should be allowed to open these covers.

This symbol indicates an ultraviolet light (UV) caution.

This symbol indicates that caution is required. Refer to product documentation for

details.

This symbol indicates a hot surface.

This symbol indicates an earth.

This symbol means “ON” for a power switch.

This symbol means “OFF” for a power switch.

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ENVIRONMENTAL CONDITIONS

This equipment is designed to be safe at least under the following conditions (based on the IEC

61010-1):

■ Indoor use;

■ Altitude up to 2000 m;

■ Ambient temperature 5

■ Maximum relative humidity 80% for temperature up to 31

at 40

■ Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage;

■ Other supply voltage fluctuations as stated by the manufacturer;

■ Transient overvoltages according to Installation Categories (Overvoltage Categories) II; For mains

supply the minimum and normal category is II;

■ Pollution degree 2 in accordance with IEC 60664.

C to 40oC

C decreasing linearly to 50% relative humidity

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INCUBATOR COMPONENTS

Handles

9 (inside)

19,20 (inside)

For MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

Handles

10, 11

For MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

Rear Right Side

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Rear Left Side

1. Outer door: Sticks to frame with magnetic seal. Door heater is installed in the outer door panel. The door opening is reversible. Contact Sanyo representative or agent to change the door hinge from left to right or vice versa.

2. Inner door: Made of tempered glass, however avoid excessive impact on the glass.

3. Leveling foot: Screw type for adjusting the height. Adjust the foot so that the incubator can be

level.

4. Tray: Can be pulled toward you.

5. Tray support: Can be removed by lifting the front side and pulling toward you.

6. Side support: Right and left side supports can be removed for disinfection. See page 27, 28 and

29.

7. Fan cover: Inlet of circulating air. Removable.

8. Duct: Flow path for circulating air. Removable.

9. Fan (inside the rear duct): Made from polypropylene resin. Can be disinfected by an autoclave.

10. Sample air outlet: This also functions as an internal gas outlet. Normally, cover this outlet with

the sample air outlet cap.

11. Sample air outlet cap: Always attach this cap except at the time of using of sample air outlet.

Note: Operation without sample air outlet cap may result low humidity or condensation on the outer door.

12. Connecting port for CO

gas pipe: When an optional component MCO-21GC (automatic CO

cylinder changeover system) is installed, both A and B are available. If MCO-21GC is not used, only A is available. Refer to page 14 for gas cylinder connection. Ensure that the gas pressure is set at 0.03 MPaG (0.3 kgf/cm

G, 4.3 psiG). Refer to page 39 for automatic CO2 cylinder changeover system.

13. Door switch: Detects the door opening/closing and stops the circulating fan, UV lamp* and electromagnetic valve for CO

when door is open.

14. Humidifying pan: Use sterile distilled water to fill the pan. (MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed) Install the pan properly so that it can be covered with the pan cover.

15. Remote alarm terminal: Refer to page 11.

16. Access port: When not in use, cap with the silicon caps on both outside and inside.

17. Power switch: Main switch of the incubator. Also functions as an over-current breaker. The

switch is covered by a switch cover to prevent the accidental push. To turn on or off the switch, remove the switch cover by loosening the screw.

18. Humidifying pan cover*: Prevents UV light being exposed to the chamber. When filling the pan, lift the front side and take out the pan. See page 30 for details.

19. UV lamp*: Sanyo UV lamp does not generate ozone. Never look at the UV light directly. For replacement, contact Sanyo sales representative or agent.

20. Water level sensor for humidifying pan*: Detects the water level in the humidifying pan. See page 24 for details.

21. Glow starter*: For UV lamp (model; FG-7P)

*MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

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Control panel and keypad

3 4 5 6 7

10 11

151617

1. Digital temperature indicator (TEMPERATURE

C): Normally, this indicator shows the chamber temperature. In the setting mode, it shows the set value of the chamber temperature. If the self diagnostic function detects any abnormality, an error code will be displayed.

2. Heater lamp (HEAT): This lamp lights when the heater is energized.

3. UV indicator (UV): This lamp lights when the UV lamp is ON. The blink of this indicator

recommends the replacement of UV lamp. See page 19 for the details. MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

4. Door lamp (DOOR): This lamp lights when the outer door is open.

5. Water level alarm lamp (RH PAN): This lamp blinks when the water in the humidifying pan is less

than approximately 0.8 liter. MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

6. Upper limit regulator: This regulator is used to set the upper temperature limit.

7. Over heat lamp (OVER HEAT): This lamp lights when the chamber temperature reaches the upper

limit set value.

8. CO

inject lamp (INJECT): This lamp lights when CO2 gas is being injected.

9. Digital CO

chamber. In the setting mode, it indicates the set value of the CO mode, the decimal point in the digital CO

density indicator (CO2 %): Normally, this indicator shows the CO2 concentration in the

concentration. In the calibration

density indicator blinks.

10. Set key (SET): Pressing this key to enter the setting mode, and the digits to be set will blink.

11. Calibration key (CAL): Pressing this key to enter the CO

calibration pre-conditioning mode.

Normally when the outer door is opened, the fan motor will stop operating. This mode allows the fan

motor to continue to circulate the chamber environment to ensure accurate CO

uniformity inside the

chamber. At this time, by pressing this key for approximately 5 seconds, the incubator enters the

temperature calibration input mode. And then pressing this key again, the incubator enters the CO

calibration input mode. See page 31 and 32 for the details. Also, used to change the UV lamp ON

period. See page 21 for the details.

Note:

This operation affects the basic performance. When calibrating the CO

density, contact a Sanyo sales

representative or agent.

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12. Alarm buzzer stop key (BUZZER): Press this key to silence the buzzer when the alarm operates and the buzzer sounds (The remote alarm is not stopped).

13. CO

provided that MCO-21GC automatic CO

14. CO

an automatic CO is empty, the CO

gas supply line indicator (A/B): The lamp for the supply line currently in use lights up

cylinder changeover system (option) is installed.

gas supply line switching key : This key to select CO2 gas supply line is available only when

cylinder changeover system MCO-21GC (option) is installed. When one CO2cylinder

is supplied by the other cylinder automatically.

15. Enter key (ENT): Pressing this key memorizes the set value in the controller.

16. Numerical value shift key ( ): Pressing this key in the setting mode causes the numerical value

to shift. In key lock mode, pressing this key makes key lock ON or OFF. See page 23 for details.

17. Digit shift key ( ): Pressing this key in the setting mode causes the changeable digit to shift. Pressing this key more than 5 seconds enters key lock mode. See page 23 for the key lock.

Remote alarm terminal

The terminal of the remote alarm is installed at the rear right side of the incubator. The alarm is

outputted from this terminal. Contact capacity is DC 30 V, 2 A.

Contact output:

between COM. and N.O. between COM. and N.C.

At normal Open Close

At abnormal Close Open

Note:

The alarm is actuated when the power cord is disconnected from the outlet or the power switch is OFF.

• The remote alarm cannot be stopped by pressing the alarm buzzer stop key (BUZZER) since the

remote alarm is not conjunct with the alarm buzzer stop key (BUZZER).

Remote alarm terminal

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INSTALLATION SITE

To operate this incubator properly and to obtain maximum performance, install the incubator in a location with the following conditions:

WARNING

Ventilate a room air occasionally when using CO2 gas for control. The gas density will increase in

an enclosed small room and high level of gas density can be hazardous to health. In addition, avoid

inhaling the chamber air directly when opening the door if CO

Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO

être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la

porte.

■ A location not subjected to direct sunlight

Do not install the incubator under direct sunlight. Installation in a location subjected to direct sunlight

cannot obtain the intended performance.

■ A location away from heat generating sources

Avoid installing the incubator near heat-emitting appliances such as a heater or a boiler etc. Heat can

decrease the intended performance of the incubator.

gas is used.

de l’air peut s’élever et peut

■ 5

C higher than the ambient temperature

The chamber temperature must be at least 5

chamber temperature is set to 37

ambient temperature in adequate range.

■ A location with a sturdy and level floor

Always install the incubator on a sturdy and level floor. The uneven floor or tilted installation may cause

failure or injury. Install the incubator in stable condition to avoid the vibration or noise. Unstable

condition may cause vibration or noise.

C, the ambient temperature must be less than 32oC. Keep the

C higher than the ambient temperature. For example, the

WARNING

Install the incubator on a sturdy floor. If the floor is not strong enough or the installation site is not

adequate, this may result in injury from the incubator falling or tipping over.

Select a level and sturdy floor for installation. This precaution will prevent the incubator from tipping.

Improper installation may result in water spillage or injury from the incubator tipping over.

■ A location not prone to high humidity

Install the incubator in the ambient of 80% R.H. or less humidity. Installation under high humidity may

cause current leakage or electric shock.

WARNING

Do not use the incubator outdoors. Current leakage or electric shock may result if the incubator is

exposed to rain water.

Never install the incubator in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

■ A location without flammable or corrosive gas

Never install the incubator in a flammable or corrosive location. This may cause explosion or fire or may

result in the current leakage or electric shock by the corrosion of the electrical components.

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INSTALLATION

1. Remove the packaging materials and tapes

Remove all transportation packaging materials and tapes. Open the doors and ventilate the incubator.

If the outside panels are dirty, clean them with a neutral detergent and wipe it up with a wet cloth.

WARNING

Do not put the packing plastic bag within reach of children as suffocation may result.

2. Adjust the leveling feet

Extend the leveling feet by rotating them counterclockwise to contact them to the floor. Ensure the

incubator is level.

3. Fix the incubator

Two fixtures are attached to the rear of the frame. Fix the frame to the wall with these hooks and rope or

chain.

4. Ground (earth)

WARNING

Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet is

not grounded, it is necessary to install a ground by qualified engineers.

Never ground the incubator through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

● Stacked module

When the product is used in stacked module, make sure to fix the incubator by using the stacking kit

WARNING

Do not climb onto the incubator and do not put articles on the incubator. This may cause injury by

tipping or damage to the incubator. When stacking the incubator, follow the procedure shown on page

40.

● If not used

When the incubator is not used, dispose the water in the humidifying pan and remove any moisture in the

chamber completely. Check that the chamber is completely dry before closing the doors.

● Before moving the incubator

Empty the humidifying pan completely before moving the incubator. Spilled or splashed water may

cause current leakage or electric shock.

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