Sanyo CYTOGROW GLP User Manual

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CytoGROW™ GLP Series Cell Culture CO
Incubator
2
MCO-18AC MCO-18AC(UV) MCO-36AC MCO-36AC(UV)
Incubation
Available with optional exclusive SafeCell™ UV decontamination system.
Reliable and accurate CO2 control, uniform temperature and humidity, contamination-resistant interior and easy to use controls.
www.sanyobiomedical.com
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MCO-18AC
MCO-36AC
MCO-18AC(UV)
MCO-36AC(UV)
CytoGROW™ GLP Series Cell Culture CO2 Incubator
Ideal for research and clinical microbiology, the SANYO CytoGROW™ GLP CO2 incubator offers a high-performance solution for mainstream cell culture applications in the research and clinical environment where precise control and contamination resistance is critical.
The CytoGROW™ GLP Series Advantage At-A-Glance
System Feature Competitors SANYO Benefit
Contamination Resistance In Situ
Air and Water Decontamination In Situ
Cabinet Construction
Active Background
Contamination Control
with inCu saFe
enriched stainless
SafeCell
Ease-of-use Direct Heat
and Air Jacket
steel interior
UV Protection
(Optional)
copper-
design
Stainless steel InCu saFe
Not available SafeCell™ UV protection located safely below the interior base destroys airborne
Composite
direct heat or
water jacket
steel does not offer a similar latent protection.
contaminants as they pass over the humidity reservoir surface. Pathogens introduced during door openings are ultimately removed.
The SANYO combination of inCu saFe™ and SafeCell™ UV eliminates the need for time­consuming, disruptive heat decontamination. SANYO minimizes downtime for total cleaning when required, with the benefit of continuous, preventive contamination control during normal operation.
The SA NYO CytoGROW of a water-jacketed cabinet without the inconvenience. SANYO uses sensitivity to ambient temperatures to permit the microprocessor-controlled, multi-zone S ANYO Direct Heat and Air Jacket maximum temperature control and uniformity from three independent heating zones on all sides.
forms integral germicidal barrier against airborne contaminants; stainless
GLP series cabinet provides the stability and uniformity
system to work most efficiently. SANYO maintains
Product Overview
CytoGROW™ GLP series is part of the SANYO MCO incubator product group that share proven control, construction and functional performance attributes. This relational product line extends the most sophisticated SANYO operating systems, offering more range of application and better continuity among users in the research and clinical community.
2 CytoGROW™ GLP Series Cell Culture CO2 Incubator www.sanyobiomedical.com
The 6 cu.ft. (170 liter) CytoGROW™ GLP cabinet integrates SANYO’s proven package of control, heating, gas control and construction features essential to all incubators. This design approach fulfills a broad range of common applications.
Additionally, the standard CytoGROW
GLP cabinet can be enhanced with a selection of options and accessories to meet specific end user needs, offering the industry’s most flexible investment option for current and future incubator needs.
MC O-18AC
Ideal for Research and Clinical Microbiology
Biotech Research and Production• Hospitals and Clinics• Public Health• Medical Research•
Quality Control•
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Incubator Features and Benefits
The SANYO CytoGROW™ GLP series is a full-featured CO2 incubator designed to establish and maintain a critical temperature, CO2 and elevated humidity environment essential to mammalian cell culture and related scientific investigation.
Digital LED display
6
4
3
5
2
8
1
7
Model MCO-18AC is designed for use with a variety of standard cell culture vessels and protocols. Three adjustable shelves are included, standard; maximum shelf capacity is 15 shelves. Microprocessor control is center-mounted in door for easy access, and reversible door swing.
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Product Features
1. SANYO exclusive patented SafeCell™ UV system available as an option
2. InCu saFe
copper-enriched polished antimicrobial stainless steel alloy interior
3. Patented Direct Heat and Air Jacket heating system and construction
4. Thermal conductivity CO with micro-processor for CO
sensor
2
2
recovery without overshoot
5. Outer door heater is integrated with the Direct Heat and Air Jacket
controller to eliminate condensation on the glass inner door
6. Door-mounted microprocessor control panel, center-mounted for easy access and to permit door reversal when required
7. Adjustable leveling feet
8. Removable interior components; no tools required
9. Rounded, electropolished interior corners for easy cleaning
Visual alarm indicator
Positive feedback tactile entry and function keys
Integrated Microprocessor Control
The CytoGROW™ GLP incubator is managed by an integrated micro­processor controller to simplify all incubator functions. Stable temperature and humidity conditions are achieved through a combination of performance systems supervised by the controller complete with alarm, calibration and diagnostic protocols.
All incubator functions are managed • by a fully integrated microprocessor controller which acquires and pro­cesses information from data entry, setpoints and alarm parameters.
A range of setpoint, alarm and • programmable inputs are established through the use of function keys.
Standard parameters are factory-set • for quick start-up, and all parameters can be changed as required.
Most parameters can be exported • to remote databases, off-site alarm or data capture systems through optional communications ports for compliance monitoring.
A remote alarm terminal at the rear • of the cabinet can be connected to an external alarm system.
Tactile feedback, touch pad data • shift and entry keys simplify operation.
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SANYO Design and Construction
Direct Heat and Air Jacket™ Heating System
Zone Location Function Energy Microprocessor Controller
Main (Red)
Base (Yel lo w)
Front (Green)
Air Jacket (White)
Insulation (Gray)
Side, top and rear walls Dominant heat source Variable
Floor
Outer door
Side, top and rear walls
Side, top and rear walls, door
Base heater elevates the humidity reservoir to achieve 95% RH at 37° C
Warms the inner glass in response to ambient conditions; eliminates condensation on glass and around the opening and promotes temperature uniformity
Sealed, surrounds interior chamber with natural air convection and promotes chamber wall uniformity
Promotes energy efficiency, mitigates effect of ambient temperature fluctuations on air jacket
Variable
Variable
– –
– –
Ease-of-Use
Direct Heat and Air Jacket™ Heating System
The patented Direct Heat and Air Jacket™ surrounds the inner walls with a natural convection airflow that converts to radiant wall heat through thermal conduction to achieve accurate, uniform and highly responsive temperature control within the chamber.
Energizes any, all or
in combination of heating
elements as required.
The microprocessor controller directs • proportional distribution of power to independent heating sources sur­rounding the chamber.
Arranged in three zones, these sources • include the side, top and rear walls, the chamber base and the outer door.
Each zone is controlled by the micro-• processor which manages continuous feedback from the incubator chamber sensors.
FRONT SIDE
The patented Direct Heat and Air Jacket™ heating system distributes proportional energy to the interior chamber through a natural convection air jacket surrounded by a high-density insulation to protect against ambient temperature fluctuations.
The patented Direct Heat and Air Jacket™ conditioning system manages setpoint temperature through multiple variable warming points under microprocessor control. To avoid cell culture desiccation, the CytoGROW™ incubator maintains ~95% RH at 37°C.
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SANYO Design and Construction
InCu saFe™ Interior Surfaces for Germicidal Protection
The CytoGROW™ GLP incubators incorporate inCu saFe stainless steel alloy interior surfaces within a comprehensive design created to eliminate contamination sources and to mitigate the effect of airborne contam­inants introduced through normal use.
Selected to provide natural germicidal • protection without rust or corrosion, inCu saFe
expresses a natural ger­micidal attribute to inhibit the growth of molds, fungi, mycoplasma and bacteria.
All interior components, including • the air management plenum, shelf supports, humidity pan and blower wheel assembly remove easily for autoclaving; no tools required.
When components are removed, • all interior surfaces are exposed for conventional wipe down.
Large rounded corners and electropol-• ished surfaces are easy to clean.
copper-enriched
Effectiveness of inCu saFe™ - Mycoplasma Survival Results
Mycoplasma Strain
Mycoplasma fermentans PG18
Mycoplasma orale CH19299
Mycoplasma arginini G230
Mycoplasma hominis PG21
Chart summarizes test results with four strains of mycoplasma. Results demonstrate how SANYO inCu saFe™ copper-enriched stainless steel alloy offers germicidal properties of conventional C1100 copper, while maintaining both corrosion-proof and discoloration-resistant properties of conventional Type 304 stainless steel. Detailed test results are available from SANYO.
Ergonomic Cabinet Design
With a reversible outer door, a single SANYO incubator offers the industry's most flexible installation options avail­able in either single or dual (stacked) cabinet configurations.
The low profile cabinet with door-• mounted control panel permits easy access and display viewing.
Cabinet knock-outs are predrilled • and tapped to eliminate drilling and to simplify remounting of the field reversible door.
The outer door heater cable is • easily switched if a reverse opening is required.
ErgoStack™ Low Profile Design
The CytoGROW™ GLP cabinet is designed for stacking, allowing one unit to be positioned on top of another, doubling interior volume without additional floor space.
Negative
Control
no survival survival no survival no survival
no survival survival no survival no survival
no survival survival no survival no survival
no survival survival no survival no survival
MCO-36AC(UV)
Conventional Type
304 Stainless Steel
The outer door closes against a • soft, easily cleaned magnetic gasket designed to eliminate ambient air shear across the glass inner door, minimizing condensation.
The outer door includes a universal • finger grip at each side.
A door ajar alarm provides an audible • and visual warning if the outer door is left open.
Pass-through ports accommodate • probes or instrumentation leads as required for specialized cell culture pro­tocols. Each chamber includes a port positioned in the interior, upper-left rear wall, with dual silicone (non-VOC) stop­pers inside and outside the cabinet for added protection.
SANYO
InCu saFe
Conventional
Copper C1100
The low profile cabinet with • door-mounted control panel permits easy access and display viewing.
The combination of stacking and • reversible doors offers the industry's most flexible installation options.
An optional roller base is recom-• mended for stacked installations to permit mobility if required; see Accessories.
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SANYO Contamination Control
Integral Contamination Resistance
CytoGROW™ GLP is designed to accom­modate a variety of microbiological and cell culture protocols used in various laboratories. Because Active Background Contamination Control nation while work is in process, risk of loss due to contaminated work is minimized.
Exclusive SANYO inCu saFe• enriched stainless steel interior surfaces provide constant germicidal protection against cross contamina­tion among specimens or contami­nants introduced during routine door openings.
Wide radius, sealed rounded interior • corners and 100% removable interior components simplify cleaning and assure thorough manual decontamina­tion when required.
An optional, patented SafeCell• system scrubs interior airflow and humidity pan water to destroy airborne contaminants.
Active Background
Contamination Control
(5 min. door openings)
SafeCell
Decontamination Protocol
(24 Hours, total)
fights contami-
SafeCell™ UV
UV 24HR
copper-
UV
PREP: 15 min.
Before sterilization by UV lamp, remove all attachments (trays, tray supports, side supports, top duct, rear duct, humidifying pan, and pan cover with fan) in the chamber.
Active Background Contamination Control
The CytoGROW™ GLP series can be ordered with the optional SafeCell contamination control system which offers significant benefits by minimizing interruptions for decontamination, by improving cell culture growth and
expression under stable, repeatable conditions, and by minimizing the
UV
potential for product loss.
SafeCell•
UV includes a program­mable ultraviolet lamp, isolated from cell cultures, that sterilizes conditioned
Start Incubation
Start Cycle: 24 hours
Follow keypad function code. UV lamp is on for 24 hours.
SafeCell™ UV ON: 5 min. after door opening
Scrubs interior airflow to destroy airborne and humidity pan contaminants.
SafeCell™ UV ON: 5 min. after door opening
Scrubs interior airflow to destroy airborne and humidity pan contaminants.
SafeCell 5 min. after door opening
Scrubs interior air flow to destroy airborne and humidity pan contaminants.
Elapsed Time:
Conventional High Heat
Decontamination
(24 Hours, total)
15 min. 30 min. 45 min. 1 2
Start Cycle: 90 min.
Interior chamber elevates to high heat.
6 CytoGROW™ GLP Series Cell Culture CO2 Incubator www.sanyobiomedical.com
3
Sterilize: 14 hours
Interior chamber remains at high heat.
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SANYO Contamination Control
air and humidity reservoir water to prevent contamination without affect­ing cell cultures in vitro.
InCu saFe•
interior surfaces provide natural germicidal resistance to con­tamination.
SafeCell•
UV inhibits the growth of mycoplasma, bacteria, molds, spores, yeasts and fungi without costly HEPA filter air scrubbers which accumulate contaminants in the chamber air.
The SafeCell•
UV system can be
factory- or field-installed.
High temperature decontamination • systems, which can actually encour­age in vitro growth of heat resistant thermophilic and hyperthermophilic microorganisms, are avoided.
Optional SafeCell™UV System
At the base of the plenum an isolated beam of high intensity, ozone-free ultra­violet light destroys contaminants in the air and in the humidity water reservoir, away from active cell cultures.
Contaminants trapped within the • distilled water pan are destroyed by ultraviolet light.
Sterile, humidified air is released • from the lower plenum for vertical convection through and around the per­forated shelves. Interior air motion is suspended when the door is opened, minimizing movement of room air contaminants into the chamber.
UV light is isolated by the plenum • cover to protect cell cultures.
Airborne contaminants are eliminated • by an automatic, factory-set five-min­ute UV cycle after door opening; the cycle is programmable 0-30 minutes.
Trace contaminants that attach to • interior surfaces are destroyed by the passive germicidal properties of the inCu saFe
Continuous Active Background Contamination Control™ process eliminates contamination without downtime. SafeCell™ is based on an integrated combination of narrow bandwidth, ozone-free ultraviolet light, inCu saFe™ copper-enriched stainless steel alloy interiors and Direct Heat and Air Jacket™ heating managed by the microprocessor controller. Pictured, MCO-18AC(UV) .
surfaces.
UV ON:
SafeCell™ UV ON: 5 min. after door opening
Scrubs interior airflow to destroy airborne and humidity pan contaminants.
SafeCell™ UV ON: 5 min. (every 12 hours)
Scrubs interior airflow to destroy airborne and humidity pan contaminants.
Finish.
Shelves, humidity pan and plenum are returned to operating position.
Finish.
Incubator must cool from high heat temperatures to near ambient.
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SANYO Contamination Control
Comparative testing commissioned by SANYO and performed by a certified inde­pendent testing laboratory confirms that the SANYO UV light decontamination process “is as effective against bacteria, yeasts and molds as high heat sterilization at sustained temperatures” ranging from +90°C to +140°C offered in other incuba­tors on the market. Additionally, the SANYO incubator isolates the UV light emission from cell cultures during normal operation to permit decontamination of the inter­nal atmosphere following routine door openings without damaging cell cultures, a process which the heat sterilization technique cannot replicate. Contact SANYO for test results.
UV Light Decontamination vs. High Heat Sterilization
Method
Test Results, Maximum Log Reducations
Bacteria >4.5 >4.5 >4.5
Yeast >2.9 >2.9 >2.9
Mold >2.7 >2.7 >2.7
Decontamination Options
Overnight YES YES YES
Active Background Contamination Control
A Comparative Analysis of Ultraviolet Light Decontamination vs. High Heat Sterilization in the Cell Culture CO2 Incubator, with the Use of Copper-Enriched Stainless Steel Construction to Achieve Active Background Contamination Control H. Busujima; D. Mistry 2007
UV High Heat
YES NO NO
Available Data Communications
The microprocessor control system automatically exports performance values via an optional 4-20 mA signal for connection to auxiliary monitoring system.
Data points include temperature • and CO
density data.
2
When installed, data transfer • connectors are located at the rear of the cabinet.
Accurate CO2 Control
The automatic CO2 control system delivers precise, reliable and repeatable CO
control.
2
The • CO
setpoint is adjustable
2
from 0 to 20%.
A • CO
sample port is integrated
2
into the inner door.
Precise Temperature Control with Elevated Relative Humidity
The patented Direct Heat/Air™ condition­ing system manages setpoint tempera­ture through multiple variable warming points under microprocessor control.
UV Lamp Program Cycles
Mode Function
After Door Opening
OFF If UV protection is not desired.
24 Hour Continuous ON
The SafeCell™ UV lamp cycle is factory-set for normal use, and can be reprogrammed as desired by entering parameters through the central microprocessor control panel.
UV lamp automatically ON for five minutes after door is closed decontaminates incoming room air.
•Thetimerisfactory-set, user programmable from 0- 30 minutes.
•TheUVlampautomatically cycles ON every 12 hours, if no door opening.
Useful for overnight decontam­ination prior to first use, clinical decontamination protocols between patients, or following total chamber cleaning after main­tenance or service.
Humidification is achieved by a • combined forced-air and natural evaporation method enhanced by the Direct Heat and Air Jacket
base heater; an optional optical water level indicator warns of low water in the removable humidity pan.
The humidity pan can be refilled • without removal, yet removes easily if desired.
When filled, the humidity pan • slides into place, the optional optical sensor returns to position and the optional SafeCell
UV lamp destroys any contaminants introduced during the process.
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Interior Doors and Shelves
Shelves and Inventory Management
Convenient space efficient inventory management is simplified through a system of adjustable, extendable shelves.
Inventory shelves and brackets • are formed from cooper-enriched germicidal stainless steel, removable without tools.
Shelves are perforated to permit • natural vertical air convection through and around labware.
Three shelves are supplied standard; • additional shelves are available.
Inner Door and Gasket
The inner chamber design is critical to successful contamination control technique.
The inner gasket body forms an • effective thermal transition between the ambient air and warm, humidified incubator atmosphere, minimizing condensation and eliminating moisture traps which can harbor contaminants.
The entire inner door gasket is • removable for cleaning and/or replacement if required.
Radiant heat from the outer door, • apportioned by the microprocessor control as a function of the patented Direct Heat and Air Jacket matically warms the inner door glass in proportion to total heat demand and condensation control.
A • CO
sample port mounted
2
through the inner door permits manual sampling of interior atmosphere without opening the cabinet.
, auto-
Field-Reversible Door
The field-reversible door allows universal installation using the left-hand hinge (standard) or a right-hand hinge modification.
The outer door includes a universal • finger grip at each side.
Mounting holes for hinge hardware • are predrilled and capped with easily removable trim plugs.
The door heater cable plugs into • the alternate connection to complete the change.
MC O-18AC
Reversible Outer Door— Flexible Installation Options
Shelves are easily arranged in 1.1"/29 mm increments. Three shelves are supplied with the MCO-18AC. Total incubator capacity is fifteen shelves.
With left- or right-swing outer • door, a single SANYO incubator offers the industry's most flexible installation options available in either single or dual (stacked) cabinet configurations.
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Specifications
CytoGROW™ GLP Cell Culture CO2 Incubator
Description With SafeCell™ UV System
Single Chamber Model Number MCO-18 AC MCO-18AC(UV)
Dual Chamber Model Number (Two Independent Units, Stacked )
Operating System
SafeCell
UV Optional Standard
CO
Sensor Thermal Conductivity, Standard
2
InCu saFe
Copper-Enriched Stainless Steel Interior Standard
Microprocessor Controller/Display, Door Mounted Standard, Centered
Direct Heat and Air
(DHA) Jacket Construction Standard
Sterilization and Decontamination
Interior UV Lamp, Programmable, Ozone Free
Germicidal Protection Standard, Copper-enriched stainless steel interior
MCO- 36AC MCO-36AC(UV)
253.7 nm wave length, 4W, Optional
Environmental Performance
Temperature Control Range +5°C above ambient to 50°C (in a 5°C to 35°C ambient)
Temperature Uniformity Deviation ±0.25°C (in 25°C ambient, setting 37°C, 5% CO
CO
Control Range 0 to 20%
2
CO
Uniformit y Deviation ±0.15% (in 25°C ambient, setting 37°C, 5% CO2, no load )
2
, no load )
2
Airflow Continuous via fan with inner door closed, gentle bottom-to-top airflow
Interior Humidity
95% RH at 37°C through evaporation via DH A
optional reflective /deflective optical low-water sensor
heating system,
Control and Interface
Temperature and CO2 Control Setpoint resolution 0.1% and 0.1°C
Display LED
Communication Optional 4-20mA connection, MCO- 420MA; optional PC interface, MTR-480
Cabinet Design and Construction
Superstructure, Exterior Cabinet and Door Galvanized steel exterior, baked-on enamel finish
Interior and Shelves 3 shelves, copper-enriched stainless steel (15 maximum)
Inner Door Tempered glass
Insulation Rigid foam polyurethane
Outer Door Reversible
Access Port Single, 1.18"/ 30 mm with silicone (non -VOC ) stoppers (upper-left rear)
Leveling Feet 4, adjustable
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3.1" (80mm)
24.8" (630mm)
24.4” (620mm)
19.3" (490mm)
4.5" (115mm)
4.9" (125mm)
22.8" (580mm)
20.6" (523mm)
34.6" (880mm)
26.2" (665mm)
.75" (20mm)
1.7” (44mm)18.4" (469mm)
Energy, Electrical and Utilities
CytoGROW™ GLP Cell Culture Incubator
Description
Maximum Power Consumption 310W
Maximum Heat Discharge 1116 kJ /h
Electrical Connection 115V,60Hz, 1 phase, NEMA 5 -15P plug
CO
Gas Connection 4 to 6 mm barbed fitting
2
CO
Gas Input P ressure 5 psi from two-stage CO2 regulator
2
CO
Gas Cylinder Switchover System Optional, MCO-21GC, internal
2
Dimensions
CytoGROW™ GLP Cell Culture Incubator
Model Number
MC O-18A C
MCO-18AC(UV)
MCO-36AC
MCO-36AC(UV)
Volume
(cu.ft. per chamber)
6.0
170 L
6.0
170 L
12.0
340 L
12.0
340 L
Exterior Dimensions
(w x f-b x h)
24.4" x 27.9" x 35.4"
620 x 710 x 90 0 mm
24.4" x 27.9" x 35.4"
620 x 710 x 90 0 mm
24.4" x 27.9" x 70.8 "
620 x 710 x 180 0 mm
24.4" x 27.9" x 70.8
620 x 710 x 180 0 mm
Interior Dimensions
(w x f-b x h)
19.3" x 20.6" x 26.2"
490 x 523 x 66 5 mm
19.3" x 20.6" x 26.2"
490 x 523 x 66 5 mm
19.3" x 20.6" x 26.2" (per chamber)
490 x 523 x 66 5 mm (per chamber)
19.3" x 20.6" x 26.2" (per chamber)
490 x 523 x 66 5 mm (per chamber)
FRONT SIDE TOP
Net Weight
(nominal)
205 lbs
93 kg
205 lbs
93 kg
410 lbs
186 kg
410 lbs
186 kg
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Accessories
SANYO Biomedical products include a broad range of accessories to meet specific application requirements. For accessories or options not listed herein, contact SANYO or your authorized SANYO sales representative.
CytoGROW™ Cell Culture Incubator
Accessory Description Model Number
UV System K it*
SafeCell Narrow-bandwidth 25 3.7nm lamp and assembly. Includes water level sensor and water pan cover.
MCO-18 UV S3
Automatic CO
CO
Cylinder Regulator, CGA fitting 320 MCO-10 0L
2
Roller Base. For use in single or stacked installations. MCO-18 RB
InCu saFe
InCu saFe
Communications Port. Located at rear of chamber. Connec tor, cable and software not supplied.* MCO-420MA
Cylinder Switchover System* (per chamber) MCO- 21GC
2
Full Shelf and Brackets. Includes two shelf brackets. MCO -47ST
Half Tray MCO-25ST
*Factory installed; specify when ordering.
Unique SANYO Services
On-site consultation•
Specialized documentation for each • individual unit
Customized testing procedures • based on personalized customer requirements
SANYO Connect
SANYO’s customer-driven biomedi­cal service program guarantees local attention from qualified SANYO service representatives, whenever and wher­ever you need it.
New Unit Installation and Training•
Predelivery and On-Site Services
Predelivery services include factory acceptance testing, calibration, and temperature mapping. On-site services include installation qualification, operational qualification, performance qualification, calibration and temperature mapping.
Preventative Maintenance•
Warranty and Non-Warranty Repairs•
Calibration/Validation Services•
Refurbishment and Reconditioning•
Customized Service and Warranty • Programs
In-Stock Parts for Immediate Delivery•
Product conforms to RoHS (European Restriction of Hazardous Substance directives)
SANYO Electric Co.,Ltd., Biomedical Division, Gumma is certified for quality management system:ISO9001/medical devices quality management system:ISO13485/environmental management system:ISO14001
UV U.S. Patent 6255103; Direct Heat and Air Jacket™ U.S. Patent 5519188;
SafeCell SafeCell™ UV, inCu saFe™, Direct Heat and Air Jacket™, P.I.D./R™ and Active Background Contamination Control™, are trademarks of SANYO Electric Biomedical Co., Ltd.
© 2010 Specifications subject to change without notice.
SANYO North America Corporation
1300 Michael Drive, Suite A, Wood Dale, IL 60191
Toll free USA (800) 858-8442, Fax (630) 238-0074
Biomedical Solutions Division
www.sanyobiomedical.com
LR.MCO-20/40AIC.6-10
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