Available with optional
exclusive SafeCell™ UV
decontamination system.
Reliable and accurate CO2 control,
uniform temperature and humidity,
contamination-resistant interior
and easy to use controls.
www.sanyobiomedical.com
Page 2
MCO-18AC
MCO-36AC
MCO-18AC(UV)
MCO-36AC(UV)
CytoGROW™ GLP Series Cell Culture CO2 Incubator
Ideal for research and clinical microbiology, the SANYO CytoGROW™ GLP CO2 incubator offers
a high-performance solution for mainstream cell culture applications in the research and clinical
environment where precise control and contamination resistance is critical.
The CytoGROW™ GLP Series Advantage At-A-Glance
SystemFeatureCompetitorsSANYO Benefit
™
Contamination
Resistance In Situ
Air and Water
Decontamination In Situ
Cabinet
Construction
Active Background
Contamination Control
with inCu saFe
enriched stainless
SafeCell
Ease-of-use Direct Heat
and Air Jacket
™
steel interior
™
UV Protection
(Optional)
™
copper-
design
Stainless steelInCu saFe
™
Not availableSafeCell™ UV protection located safely below the interior base destroys airborne
Composite
direct heat or
water jacket
steel does not offer a similar latent protection.
contaminants as they pass over the humidity reservoir surface. Pathogens introduced
during door openings are ultimately removed.
The SANYO combination of inCu saFe™ and SafeCell™ UV eliminates the need for timeconsuming, disruptive heat decontamination. SANYO minimizes downtime for total
cleaning when required, with the benefit of continuous, preventive contamination
control during normal operation.
The SA NYO CytoGROW
of a water-jacketed cabinet without the inconvenience. SANYO uses sensitivity
to ambient temperatures to permit the microprocessor-controlled, multi-zone S ANYO
Direct Heat and Air Jacket
maximum temperature control and uniformity from three independent heating
zones on all sides.
forms integral germicidal barrier against airborne contaminants; stainless
™
GLP series cabinet provides the stability and uniformity
™
system to work most efficiently. SANYO maintains
Product Overview
CytoGROW™ GLP series is part of
the SANYO MCO incubator product
group that share proven control,
construction and functional performance
attributes. This relational product line
extends the most sophisticated SANYO
operating systems, offering more range
of application and better continuity
among users in the research and
clinical community.
2 CytoGROW™ GLP Series Cell Culture CO2 Incubator www.sanyobiomedical.com
The 6 cu.ft. (170 liter) CytoGROW™ GLP
cabinet integrates SANYO’s proven
package of control, heating, gas control
and construction features essential to all
incubators. This design approach fulfills a
broad range of common applications.
Additionally, the standard CytoGROW
™
GLP cabinet can be enhanced with a
selection of options and accessories to
meet specific end user needs, offering
the industry’s most flexible investment
option for current and future incubator
needs.
MC O-18AC
Ideal for Research and
Clinical Microbiology
Biotech Research and Production•
Hospitals and Clinics•
Public Health•
Medical Research•
Quality Control•
Page 3
Incubator Features and Benefits
The SANYO CytoGROW™ GLP series is a full-featured CO2 incubator designed to establish and maintain a critical temperature,
CO2 and elevated humidity environment essential to mammalian cell culture and related scientific investigation.
Digital LED display
6
4
3
5
2
8
1
7
Model MCO-18AC is designed for use with a variety of standard cell culture vessels and protocols.
Three adjustable shelves are included, standard; maximum shelf capacity is 15 shelves. Microprocessor control
is center-mounted in door for easy access, and reversible door swing.
9
Product Features
1. SANYO exclusive patented SafeCell™
UV system available as an option
3. Patented Direct Heat and Air Jacket
heating system and construction
4. Thermal conductivity CO
with micro-processor for CO
sensor
2
2
recovery without overshoot
5. Outer door heater is integrated with
the Direct Heat and Air Jacket
™
controller to eliminate condensation
on the glass inner door
6. Door-mounted microprocessor
control panel, center-mounted
for easy access and to permit
door reversal when required
7. Adjustable leveling feet
™
8. Removable interior components;
no tools required
9. Rounded, electropolished interior
corners for easy cleaning
Visual alarm
indicator
Positive feedback tactile
entry and function keys
Integrated
Microprocessor Control
The CytoGROW™ GLP incubator
is managed by an integrated microprocessor controller to simplify all
incubator functions. Stable temperature
and humidity conditions are achieved
through a combination of performance
systems supervised by the controller
complete with alarm, calibration
and diagnostic protocols.
All incubator functions are managed •
by a fully integrated microprocessor
controller which acquires and processes information from data entry,
setpoints and alarm parameters.
A range of setpoint, alarm and •
programmable inputs are established
through the use of function keys.
Standard parameters are factory-set •
for quick start-up, and all parameters
can be changed as required.
Most parameters can be exported •
to remote databases, off-site alarm
or data capture systems through
optional communications ports
for compliance monitoring.
A remote alarm terminal at the rear •
of the cabinet can be connected to
an external alarm system.
Tactile feedback, touch pad data •
shift and entry keys simplify
operation.
Side, top and rear wallsDominant heat sourceVariable
Floor
Outer door
Side, top and rear walls
Side, top and rear walls, door
Base heater elevates the humidity reservoir
to achieve 95% RH at 37° C
Warms the inner glass in response to ambient conditions;
eliminates condensation on glass and around the opening
and promotes temperature uniformity
Sealed, surrounds interior chamber with natural
air convection and promotes chamber wall uniformity
Promotes energy efficiency, mitigates effect of
ambient temperature fluctuations on air jacket
Variable
Variable
– –
– –
Ease-of-Use
Direct Heat and Air Jacket™
Heating System
The patented Direct Heat and Air Jacket™
surrounds the inner walls with a natural
convection airflow that converts to radiant
wall heat through thermal conduction
to achieve accurate, uniform and highly
responsive temperature control within
the chamber.
Energizes any, all or
in combination of heating
elements as required.
The microprocessor controller directs •
proportional distribution of power
to independent heating sources surrounding the chamber.
Arranged in three zones, these sources •
include the side, top and rear walls,
the chamber base and the outer door.
Each zone is controlled by the micro-•
processor which manages continuous
feedback from the incubator
chamber sensors.
FRONTSIDE
The patented Direct Heat and Air Jacket™ heating
system distributes proportional energy to the interior
chamber through a natural convection air jacket
surrounded by a high-density insulation to protect
against ambient temperature fluctuations.
The patented Direct Heat and Air Jacket™ conditioning
system manages setpoint temperature through multiple
variable warming points under microprocessor control.
To avoid cell culture desiccation, the CytoGROW™
incubator maintains ~95% RH at 37°C.
4 CytoGROW™ GLP Series Cell Culture CO2 Incubator www.sanyobiomedical.com
Page 5
SANYO Design and Construction
InCu saFe™ Interior Surfaces
for Germicidal Protection
The CytoGROW™ GLP incubators
incorporate inCu saFe
stainless steel alloy interior surfaces
within a comprehensive design created
to eliminate contamination sources and
to mitigate the effect of airborne contaminants introduced through normal use.
Selected to provide natural germicidal •
protection without rust or corrosion,
inCu saFe
™
expresses a natural germicidal attribute to inhibit the growth
of molds, fungi, mycoplasma and
bacteria.
All interior components, including •
the air management plenum, shelf
supports, humidity pan and blower
wheel assembly remove easily for
autoclaving; no tools required.
When components are removed, •
all interior surfaces are exposed for
conventional wipe down.
Large rounded corners and electropol-•
ished surfaces are easy to clean.
™
copper-enriched
Effectiveness of inCu saFe™ - Mycoplasma Survival Results
Mycoplasma
Strain
Mycoplasma
fermentans PG18
Mycoplasma
orale CH19299
Mycoplasma
arginini G230
Mycoplasma
hominis PG21
Chart summarizes test results with four strains of mycoplasma. Results demonstrate how SANYO inCu saFe™
copper-enriched stainless steel alloy offers germicidal properties of conventional C1100 copper, while maintaining
both corrosion-proof and discoloration-resistant properties of conventional Type 304 stainless steel.
Detailed test results are available from SANYO.
Ergonomic Cabinet Design
With a reversible outer door, a single
SANYO incubator offers the industry's
most flexible installation options available in either single or dual (stacked)
cabinet configurations.
The low profile cabinet with door-•
mounted control panel permits easy
access and display viewing.
Cabinet knock-outs are predrilled •
and tapped to eliminate drilling and
to simplify remounting of the field
reversible door.
The outer door heater cable is •
easily switched if a reverse opening
is required.
ErgoStack™ Low Profile Design
The CytoGROW™ GLP cabinet is
designed for stacking, allowing one
unit to be positioned on top of another,
doubling interior volume without
additional floor space.
Negative
Control
no survivalsurvivalno survivalno survival
no survivalsurvivalno survivalno survival
no survivalsurvivalno survivalno survival
no survivalsurvivalno survivalno survival
MCO-36AC(UV)
Conventional Type
304 Stainless Steel
The outer door closes against a •
soft, easily cleaned magnetic gasket
designed to eliminate ambient air
shear across the glass inner door,
minimizing condensation.
The outer door includes a universal •
finger grip at each side.
A door ajar alarm provides an audible •
and visual warning if the outer door
is left open.
Pass-through ports accommodate •
probes or instrumentation leads as
required for specialized cell culture protocols. Each chamber includes a port
positioned in the interior, upper-left rear
wall, with dual silicone (non-VOC) stoppers inside and outside the cabinet for
added protection.
SANYO
InCu saFe
™
Conventional
Copper C1100
The low profile cabinet with •
door-mounted control panel permits
easy access and display viewing.
The combination of stacking and •
reversible doors offers the industry's
most flexible installation options.
An optional roller base is recom-•
mended for stacked installations
to permit mobility if required;
see Accessories.
5
Page 6
SANYO Contamination Control
Integral Contamination
Resistance
CytoGROW™ GLP is designed to accommodate a variety of microbiological and
cell culture protocols used in various
laboratories. Because Active Background
Contamination Control
nation while work is in process, risk of
loss due to contaminated work
is minimized.
Exclusive SANYO inCu saFe•
enriched stainless steel interior
surfaces provide constant germicidal
protection against cross contamination among specimens or contaminants introduced during routine door
openings.
Wide radius, sealed rounded interior •
corners and 100% removable interior
components simplify cleaning and
assure thorough manual decontamination when required.
An optional, patented SafeCell•
system scrubs interior airflow and
humidity pan water to destroy
airborne contaminants.
Active Background
Contamination Control
(5 min. door openings)
SafeCell
Decontamination Protocol
(24 Hours, total)
™
fights contami-
SafeCell™ UV
™
UV 24HR
™
copper-
™
UV
PREP: 15 min.
Before sterilization by UV lamp,
remove all attachments (trays,
tray supports, side supports, top
duct, rear duct, humidifying pan,
and pan cover with fan) in the
chamber.
Active Background
Contamination Control
The CytoGROW™ GLP series can be
ordered with the optional SafeCell
contamination control system which
offers significant benefits by minimizing
interruptions for decontamination,
by improving cell culture growth and
™
expression under stable, repeatable
conditions, and by minimizing the
™
UV
potential for product loss.
SafeCell•
™
UV includes a programmable ultraviolet lamp, isolated from
cell cultures, that sterilizes conditioned
Start Incubation
Start Cycle: 24 hours
Follow keypad function code.
UV lamp is on for 24 hours.
SafeCell™ UV ON:
5 min. after door
opening
Scrubs interior
airflow to destroy
airborne and
humidity pan
contaminants.
SafeCell™ UV ON:
5 min. after door
opening
Scrubs interior
airflow to destroy
airborne and
humidity pan
contaminants.
SafeCell
5 min. after door
opening
Scrubs interior air
flow to destroy
airborne and
humidity pan
contaminants.
Elapsed Time:
Conventional High Heat
Decontamination
(24 Hours, total)
15 min.30 min.45 min.1 2
Start Cycle: 90 min.
Interior chamber elevates to high heat.
6 CytoGROW™ GLP Series Cell Culture CO2 Incubator www.sanyobiomedical.com
3
Sterilize: 14 hours
Interior chamber remains at high heat.
456789101112131415161718192021222324
Page 7
SANYO Contamination Control
air and humidity reservoir water to
prevent contamination without affecting cell cultures in vitro.
InCu saFe•
™
interior surfaces provide
natural germicidal resistance to contamination.
SafeCell•
™
UV inhibits the growth of
mycoplasma, bacteria, molds, spores,
yeasts and fungi without costly HEPA
filter air scrubbers which accumulate
contaminants in the chamber air.
The SafeCell•
™
UV system can be
factory- or field-installed.
High temperature decontamination •
systems, which can actually encourage in vitro growth of heat resistant
thermophilic and hyperthermophilic
microorganisms, are avoided.
Optional SafeCell™UV System
At the base of the plenum an isolated
beam of high intensity, ozone-free ultraviolet light destroys contaminants in the
air and in the humidity water reservoir,
away from active cell cultures.
Contaminants trapped within the •
distilled water pan are destroyed by
ultraviolet light.
Sterile, humidified air is released •
from the lower plenum for vertical
convection through and around the perforated shelves. Interior air motion is
suspended when the door is opened,
minimizing movement of room air
contaminants into the chamber.
UV light is isolated by the plenum •
cover to protect cell cultures.
Airborne contaminants are eliminated •
by an automatic, factory-set five-minute UV cycle after door opening; the
cycle is programmable 0-30 minutes.
Trace contaminants that attach to •
interior surfaces are destroyed by the
passive germicidal properties of the
inCu saFe
Continuous Active Background Contamination
Control™ process eliminates contamination without
downtime. SafeCell™ is based on an integrated
combination of narrow bandwidth, ozone-free
ultraviolet light, inCu saFe™ copper-enriched stainless
steel alloy interiors and Direct Heat and Air Jacket™
heating managed by the microprocessor controller.
Pictured, MCO-18AC(UV) .
™
surfaces.
™
UV ON:
SafeCell™ UV ON:
5 min. after door
opening
Scrubs interior
airflow to destroy
airborne and
humidity pan
contaminants.
SafeCell™ UV ON:
5 min. (every 12 hours)
Scrubs interior
airflow to destroy
airborne and humidity
pan contaminants.
Finish.
Shelves, humidity pan and plenum
are returned to operating position.
Finish.
Incubator must cool from
high heat temperatures to
near ambient.
7
Page 8
SANYO Contamination Control
Comparative testing commissioned by SANYO and performed by a certified independent testing laboratory confirms that the SANYO UV light decontamination
process “is as effective against bacteria, yeasts and molds as high heat sterilization
at sustained temperatures” ranging from +90°C to +140°C offered in other incubators on the market. Additionally, the SANYO incubator isolates the UV light emission
from cell cultures during normal operation to permit decontamination of the internal atmosphere following routine door openings without damaging cell cultures, a
process which the heat sterilization technique cannot replicate. Contact SANYO for
test results.
UV Light Decontamination vs. High Heat Sterilization
Method
Test Results, Maximum Log Reducations
Bacteria>4.5>4.5>4.5
Yeast>2.9>2.9>2.9
Mold>2.7>2.7>2.7
Decontamination Options
OvernightYESYESYES
Active Background Contamination Control
A Comparative Analysis of Ultraviolet Light Decontamination vs. High Heat Sterilization in the Cell Culture
CO2 Incubator, with the Use of Copper-Enriched Stainless Steel Construction to Achieve Active Background
Contamination Control H. Busujima; D. Mistry 2007
™
UVHigh Heat
YESNONO
Available Data Communications
The microprocessor control system
automatically exports performance
values via an optional 4-20 mA signal
for connection to auxiliary monitoring
system.
Data points include temperature •
and CO
density data.
2
When installed, data transfer •
connectors are located at the
rear of the cabinet.
Accurate CO2 Control
The automatic CO2 control system
delivers precise, reliable and repeatable
CO
control.
2
The •CO
setpoint is adjustable
2
from 0 to 20%.
A • CO
sample port is integrated
2
into the inner door.
Precise Temperature Control
with Elevated Relative Humidity
The patented Direct Heat/Air™ conditioning system manages setpoint temperature through multiple variable warming
points under microprocessor control.
UV Lamp Program Cycles
ModeFunction
After Door
Opening
OFFIf UV protection is not desired.
24 Hour
Continuous
ON
The SafeCell™ UV lamp cycle is factory-set for
normal use, and can be reprogrammed as desired
by entering parameters through the central
microprocessor control panel.
UV lamp automatically ON
for five minutes after door
is closed decontaminates
incoming room air.
•Thetimerisfactory-set,
user programmable from
0- 30 minutes.
•TheUVlampautomatically
cycles ON every 12 hours,
if no door opening.
Useful for overnight decontamination prior to first use, clinical
decontamination protocols
between patients, or following
total chamber cleaning after maintenance or service.
Humidification is achieved by a •
combined forced-air and natural
evaporation method enhanced by
the Direct Heat and Air Jacket
™
base
heater; an optional optical water
level indicator warns of low water
in the removable humidity pan.
The humidity pan can be refilled •
without removal, yet removes
easily if desired.
When filled, the humidity pan •
slides into place, the optional optical
sensor returns to position and the
optional SafeCell
™
UV lamp destroys
any contaminants introduced
during the process.
8 CytoGROW™ GLP Series Cell Culture CO2 Incubator www.sanyobiomedical.com
Page 9
Interior Doors and Shelves
Shelves and Inventory
Management
Convenient space efficient inventory
management is simplified through a
system of adjustable, extendable
shelves.
Inventory shelves and brackets •
are formed from cooper-enriched
germicidal stainless steel, removable
without tools.
Shelves are perforated to permit •
natural vertical air convection through
and around labware.
Three shelves are supplied standard; •
additional shelves are available.
Inner Door and Gasket
The inner chamber design is critical to
successful contamination control
technique.
The inner gasket body forms an •
effective thermal transition between
the ambient air and warm, humidified
incubator atmosphere, minimizing
condensation and eliminating moisture
traps which can harbor contaminants.
The entire inner door gasket is •
removable for cleaning and/or
replacement if required.
Radiant heat from the outer door, •
apportioned by the microprocessor
control as a function of the patented
Direct Heat and Air Jacket
matically warms the inner door
glass in proportion to total heat
demand and condensation control.
A • CO
sample port mounted
2
through the inner door permits
manual sampling of interior
atmosphere without opening
the cabinet.
™
, auto-
Field-Reversible Door
The field-reversible door allows
universal installation using the
left-hand hinge (standard) or a
right-hand hinge modification.
The outer door includes a universal •
finger grip at each side.
Mounting holes for hinge hardware •
are predrilled and capped with
easily removable trim plugs.
The door heater cable plugs into •
the alternate connection to complete
the change.
Shelves are easily arranged in 1.1"/29 mm
increments. Three shelves are supplied with the
MCO-18AC. Total incubator capacity is fifteen
shelves.
With left- or right-swing outer •
door, a single SANYO incubator
offers the industry's most flexible
installation options available in
either single or dual (stacked)
cabinet configurations.
9
Page 10
Specifications
CytoGROW™ GLP Cell Culture CO2 Incubator
DescriptionWith SafeCell™ UV System
Single Chamber Model NumberMCO-18 ACMCO-18AC(UV)
Dual Chamber Model Number
(Two Independent Units, Stacked )
Operating System
™
SafeCell
UVOptionalStandard
CO
SensorThermal Conductivity, Standard
2
InCu saFe
™
Copper-Enriched Stainless Steel InteriorStandard
Microprocessor Controller/Display, Door MountedStandard, Centered
Temperature and CO2 ControlSetpoint resolution 0.1% and 0.1°C
DisplayLED
CommunicationOptional 4-20mA connection, MCO- 420MA; optional PC interface, MTR-480
Cabinet Design and Construction
Superstructure, Exterior Cabinet and DoorGalvanized steel exterior, baked-on enamel finish
Interior and Shelves3 shelves, copper-enriched stainless steel (15 maximum)
Inner DoorTempered glass
InsulationRigid foam polyurethane
Outer DoorReversible
Access PortSingle, 1.18"/ 30 mm with silicone (non -VOC ) stoppers (upper-left rear)
Leveling Feet4, adjustable
10 CytoGROW™ GLP Series Cell Culture CO2 Incubator www.sanyobiomedical.com
Page 11
3.1" (80mm)
24.8" (630mm)
24.4” (620mm)
19.3" (490mm)
4.5" (115mm)
4.9" (125mm)
22.8" (580mm)
20.6" (523mm)
34.6" (880mm)
26.2" (665mm)
.75" (20mm)
1.7” (44mm)18.4" (469mm)
Energy, Electrical and Utilities
CytoGROW™ GLP Cell Culture Incubator
Description
Maximum Power Consumption310W
Maximum Heat Discharge1116 kJ /h
Electrical Connection115V,60Hz, 1 phase, NEMA 5 -15P plug
CO
Gas Connection4 to 6 mm barbed fitting
2
CO
Gas Input P ressure5 psi from two-stage CO2 regulator
2
CO
Gas Cylinder Switchover SystemOptional, MCO-21GC, internal
2
Dimensions
CytoGROW™ GLP Cell Culture Incubator
Model Number
MC O-18A C
MCO-18AC(UV)
MCO-36AC
MCO-36AC(UV)
Volume
(cu.ft. per chamber)
6.0
170 L
6.0
170 L
12.0
340 L
12.0
340 L
Exterior Dimensions
(w x f-b x h)
24.4" x 27.9" x 35.4"
620 x 710 x 90 0 mm
24.4" x 27.9" x 35.4"
620 x 710 x 90 0 mm
24.4" x 27.9" x 70.8 "
620 x 710 x 180 0 mm
24.4" x 27.9" x 70.8
620 x 710 x 180 0 mm
Interior Dimensions
(w x f-b x h)
19.3" x 20.6" x 26.2"
490 x 523 x 66 5 mm
19.3" x 20.6" x 26.2"
490 x 523 x 66 5 mm
19.3" x 20.6" x 26.2" (per chamber)
490 x 523 x 66 5 mm (per chamber)
19.3" x 20.6" x 26.2" (per chamber)
490 x 523 x 66 5 mm (per chamber)
FRONTSIDETOP
Net Weight
(nominal)
205 lbs
93 kg
205 lbs
93 kg
410 lbs
186 kg
410 lbs
186 kg
11
Page 12
Accessories
SANYO Biomedical products include a broad range of accessories to meet specific application requirements. For accessories or options
not listed herein, contact SANYO or your authorized SANYO sales representative.
CytoGROW™ Cell Culture Incubator
Accessory DescriptionModel Number
™
UV System K it*
SafeCell
Narrow-bandwidth 25 3.7nm lamp and assembly. Includes water level sensor and water pan cover.
MCO-18 UV S3
Automatic CO
CO
Cylinder Regulator, CGA fitting 320MCO-10 0L
2
Roller Base. For use in single or stacked installations.MCO-18 RB
InCu saFe
InCu saFe
Communications Port. Located at rear of chamber. Connec tor, cable and software not supplied.*MCO-420MA
Full Shelf and Brackets. Includes two shelf brackets.MCO -47ST
™
Half TrayMCO-25ST
*Factory installed; specify when ordering.
Unique SANYO Services
On-site consultation•
Specialized documentation for each •
individual unit
Customized testing procedures •
based on personalized customer
requirements
SANYO Connect
SANYO’s customer-driven biomedical service program guarantees local
attention from qualified SANYO service
representatives, whenever and wherever you need it.
New Unit Installation and Training•
Predelivery and On-Site Services
Predelivery services include factory
acceptance testing, calibration, and
temperature mapping. On-site services
include installation qualification,
operational qualification, performance
qualification, calibration and temperature
mapping.
Preventative Maintenance•
Warranty and Non-Warranty Repairs•
Calibration/Validation Services•
Refurbishment and Reconditioning•
Customized Service and Warranty •
Programs
In-Stock Parts for Immediate Delivery•
Product conforms to RoHS
(European Restriction of Hazardous
Substance directives)
SANYO Electric Co.,Ltd., Biomedical Division, Gumma is certified for quality management
system:ISO9001/medical devices quality management system:ISO13485/environmental
management system:ISO14001
™
UV U.S. Patent 6255103; Direct Heat and Air Jacket™ U.S. Patent 5519188;
SafeCell
SafeCell™ UV, inCu saFe™, Direct Heat and Air Jacket™, P.I.D./R™ and Active Background
Contamination Control™, are trademarks of SANYO Electric Biomedical Co., Ltd.