MAURICE

ProteinSimple MAURICE Users Manual

page 1

User Guide for Maurice, Maurice C. and Maurice S.

ProteinSimple 3001 Orchard Parkway San Jose, CA 95134 Toll-free: (888) 607-9692 Tel: (408) 510-5500 Fax: (408) 510-5599 email: support@proteinsimple.com web: www.proteinsimple.com

User Guide for Maurice, Maurice C. and Maurice S.

P/N 046-295

Revision 1, February 2016

For research use only. Not for use in diagnostic procedures

Patents and Trademarks

ProteinSimple's Maurice and iCE technology is covered by issued and pending patents in the U.S. and other countries. For more information please see http://www.proteinsimple.com/Intellectual_Property.html.

ProteinSimple and the ProteinSimple logo are trademarks or registered trademarks of ProteinSimple. Other marks appearing in these materials are marks of their respective owners.

Table of Contents

page 1

Chapter 1:

Let’s Get Started. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Maurice Systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Chapter 2:

Getting Your Lab Ready . . . . . . . . . . . . . . . . . . . . 3

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Physical Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Environmental Requirements. . . . . . . . . . . . . . . . . . . . . . . . . 5

Software and Computer Requirements . . . . . . . . . . . . . . . 6

General Guidelines and Information. . . . . . . . . . . . . . . . . . 6

Intended Use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Lifting and Moving the System: Lift Maurice

Correctly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Chapter 3:

Maurice. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Maurice Systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

External Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

System Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Status Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10

Internal Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Cartridge Slot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10

Sample and Reagent Platform . . . . . . . . . . . . . . . . . . 12

Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

System Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14

Computer Workstation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Compass for iCE Overview . . . . . . . . . . . . . . . . . . . . . . . . . . .18

Changing the Screen View . . . . . . . . . . . . . . . . . . . . . . 19

Batch Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Run Summary Screen . . . . . . . . . . . . . . . . . . . . . . . . . . .20

Analysis Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22

Screen Panes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24

Title Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Main Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Instrument Status Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Screen Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25

View Bar. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Compass for iCE Status Bar. . . . . . . . . . . . . . . . . . . . . . 25

Software Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25

File Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26

Edit Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

View Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

Instrument Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

Window Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Help Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28

Changing the Compass for iCE Main Window

Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Resizing the Main Compass for iCE Window . . . . 28

Resizing the Screen Tab . . . . . . . . . . . . . . . . . . . . . . . . .29

Resizing Screen Panes . . . . . . . . . . . . . . . . . . . . . . . . . . .29

Changing the Location of Screen Panes. . . . . . . . .30

Restoring the Main Window to the Default

Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32

Software Help. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32

Chapter 4:

Compass for iCE Overview . . . . . . . . . . . . . . . . 17

Launching Compass for iCE . . . . . . . . . . . . . . . . . . . . . . . . . 18

User Guide for Maurice, Maurice C. and Maurice S.

Checking for and Installing New Versions of Compass

for iCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Viewing Release Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32

page 2

Viewing the Software Log. . . . . . . . . . . . . . . . . . . . . . . . . . . .33

Compass for iCE Version Information . . . . . . . . . . . . . . . . 33

Directory and File Information. . . . . . . . . . . . . . . . . . . . . . . 34

File Types. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Chapter 5:

cIEF Batches. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Batch Screen Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

Batch Screen Panes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

Software Menus Active in the Batch Screen . . . . .39

Opening a Batch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

Creating a New Batch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Step 1 - Open a Template Batch. . . . . . . . . . . . . . . . .42

Step 2 - Assign Your Samples. . . . . . . . . . . . . . . . . . . .43

Step 3 - Assign Your Method Parameters. . . . . . . .46

Step 4 - Set Up Your Injections. . . . . . . . . . . . . . . . . . .49

Step 5 - Add Batch Notes (Optional). . . . . . . . . . . . .51

Step 6 - Modify Default Analysis Parameters

(Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Step 7 - Save Your Batch . . . . . . . . . . . . . . . . . . . . . . . .52

Viewing Replicate Injections . . . . . . . . . . . . . . . . . . . . . . . . . 53

Batch History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54

Making Changes to a Batch . . . . . . . . . . . . . . . . . . . . . . . . .55

Viewing and Editing Batches in Completed Runs . . . .56

Batch Reports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57

Step 1 - Open a Template Batch. . . . . . . . . . . . . . . . .64

Step 2 - Assign Your Samples. . . . . . . . . . . . . . . . . . . .65

Step 3 - Assign Your Method Parameters . . . . . . . .68

Step 4 - Set Up Your Injections. . . . . . . . . . . . . . . . . . .70

Step 5 - Add Batch Notes (Optional). . . . . . . . . . . . .73

Step 6 - Modify Default Analysis Parameters

(Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

Step 7 - Save Your Batch . . . . . . . . . . . . . . . . . . . . . . . .74

Viewing Replicate Injections . . . . . . . . . . . . . . . . . . . . . . . . .75

Batch History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76

Making Changes to a Batch . . . . . . . . . . . . . . . . . . . . . . . . .77

Viewing and Editing Batches in Completed Runs . . . .78

Batch Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79

Chapter 7: Running cIEF Applications on

Maurice and Maurice C. . . . . . . . . . . . . . . . . . . . 81

Before You Throw the Switch . . . . . . . . . . . . . . . . . . . . . . . .82

Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

Running cIEF Applications . . . . . . . . . . . . . . . . . . . . . . . . . . .82

What You’ll Need. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82

Step 1: Prep Your Markers, Samples and

Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83

Step 2: Prep the Cartridge . . . . . . . . . . . . . . . . . . . . . . .86

Step 3: Install the Cartridge. . . . . . . . . . . . . . . . . . . . . .88

Step 4: Load Samples and Reagents. . . . . . . . . . . . .90

Chapter 6:

CE-SDS Batches. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Batch Screen Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60

Batch Screen Panes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60

Software Menus Active in the Batch Screen . . . . .61

Opening a Batch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63

Creating a New Batch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

User Guide for Maurice, Maurice C. and Maurice S.

Step 5: Create a Batch . . . . . . . . . . . . . . . . . . . . . . . . . . .91

Step 6: Start the Batch. . . . . . . . . . . . . . . . . . . . . . . . . 100

Post-batch Procedures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

Checking Your Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

Step 1: Select Your Detection Mode . . . . . . . . . . . 105

Step 2: Check Your pI Markers . . . . . . . . . . . . . . . . . 105

page 3

Step 3: Checking Sample Peaks . . . . . . . . . . . . . . . .108

Step 4: Assigning Peak Names . . . . . . . . . . . . . . . . .111

Chapter 8: Running CE-SDS Applications on

Maurice and Maurice S. . . . . . . . . . . . . . . . . . . .112

Before You Throw the Switch . . . . . . . . . . . . . . . . . . . . . . .113

Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113

Running CE-SDS Applications . . . . . . . . . . . . . . . . . . . . . .113

What You’ll Need . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113

Step 1: Prep Your Internal Standard, Samples and

Reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114

Internal Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114

Step 2: Prep the Cartridge . . . . . . . . . . . . . . . . . . . . . .120

Step 3: Install the Cartridge. . . . . . . . . . . . . . . . . . . . .122

Step 4: Load Samples and Reagents . . . . . . . . . . .124

Step 5: Create a Batch. . . . . . . . . . . . . . . . . . . . . . . . . .125

Step 6: Start the Batch. . . . . . . . . . . . . . . . . . . . . . . . . .133

Post-batch Procedures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .135

Opening One Run File. . . . . . . . . . . . . . . . . . . . . . . . . 156

Opening Multiple Run Files. . . . . . . . . . . . . . . . . . . . 156

Batch Injection Information . . . . . . . . . . . . . . . . . . . . . . . 157

Injection Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158

Run Status Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159

Viewing the Focus Series (cIEF Only) . . . . . . . . . . . . . . . 161

Viewing the Separation (CE-SDS Only) . . . . . . . . . . . . . 162

Current and Voltage Plots. . . . . . . . . . . . . . . . . . . . . . . . . . 163

Run History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165

Viewing Multiple Events. . . . . . . . . . . . . . . . . . . . . . . 165

Copying History Info . . . . . . . . . . . . . . . . . . . . . . . . . . 167

Viewing Run Errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167

Injection Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168

Example Analysis and Injection Report: CE-SDS169

Example Analysis and Injection Report: cIEF. . . 172

Switching Between Open Run Files . . . . . . . . . . . . . . . . 174

Closing Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175

Checking Your Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139

Step 1: Check Your Internal Standard . . . . . . . . . .139

Step 2: Set Your Molecular Weight (MW)

Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .143

Step 3: Checking Sample Peaks . . . . . . . . . . . . . . . .147

Step 4: Assigning Peak Names . . . . . . . . . . . . . . . . .150

Chapter 9:

Run Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .151

Run Summary Screen Overview. . . . . . . . . . . . . . . . . . . . .152

Run Summary Screen Panes . . . . . . . . . . . . . . . . . . .152

Software Menus Active in the Run Summary

Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .154

Opening Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .156

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 10: Controlling Maurice, Maurice C. and Maurice S.

Instrument Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178

Starting a Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178

Stopping a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180

Status Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181

Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181

Instrument Software (Embedded) Updates . . . . . . . . 181

Self Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183

Viewing and Changing System Properties . . . . . . . . . 184

Checking Cartridge Status . . . . . . . . . . . . . . . . . . . . . . . . . 185

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

page 4

Viewing Log Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .186

Runs Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .186

System Logs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .187

Error Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .189

Self Test Logs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .192

Command Log. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .194

Chapter 11:

CE-SDS Data Analysis . . . . . . . . . . . . . . . . . . . . .195

Analysis Screen Overview . . . . . . . . . . . . . . . . . . . . . . . . . . .196

Analysis Screen Panes. . . . . . . . . . . . . . . . . . . . . . . . . .196

Software Menus Active in the Analysis Screen . .197

Opening Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .199

Opening One Run File. . . . . . . . . . . . . . . . . . . . . . . . . .199

Opening Multiple Run Files. . . . . . . . . . . . . . . . . . . . .199

How Run Data is Displayed . . . . . . . . . . . . . . . . . . . . . . . . .201

Experiment Pane: Batch Injection Information .201

Graph Pane: Electropherogram Data . . . . . . . . . .201

Peaks Pane: Calculated Results. . . . . . . . . . . . . . . . .202

Injections Pane: User-Specified Peak Names . . .204

Viewing Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .205

Switching Between Samples and Standards Data

Views . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .205

Selecting and Displaying Injection Data . . . . . . .208

Switching Between Single and Multiple Views of

Injections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .211

Hiding Injection Data . . . . . . . . . . . . . . . . . . . . . . . . . .213

Data Notifications and Warnings. . . . . . . . . . . . . . . . . . .214

Checking Your Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .215

Group Statistics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .216

Using Groups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .216

Viewing Sample Injection Groups . . . . . . . . . . . . . .217

Viewing Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217

Hiding or Removing Injections in Group

Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 219

Copying Results Tables and Graphs. . . . . . . . . . . . . . . . 220

Copying Results Tables . . . . . . . . . . . . . . . . . . . . . . . . 220

Copying the Graph. . . . . . . . . . . . . . . . . . . . . . . . . . . . 220

Saving the Graph as an Image File . . . . . . . . . . . . 220

Exporting Run Files. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221

Exporting Results Tables. . . . . . . . . . . . . . . . . . . . . . . 221

Exporting Raw Sample Electropherogram

Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221

Changing Sample Protein Identification . . . . . . . . . . . 222

Adding or Removing Sample Data. . . . . . . . . . . . 222

Hiding Sample Data . . . . . . . . . . . . . . . . . . . . . . . . . . 224

Changing Peak Names for Sample Data . . . . . . 225

Changing the Electropherogram View . . . . . . . . . . . . . 226

Autoscaling the Electropherogram. . . . . . . . . . . . 227

Customizing the Data Display . . . . . . . . . . . . . . . . 227

Stacking Multiple Electropherograms . . . . . . . . . 233

Overlaying Multiple Electropherograms. . . . . . . 234

Zooming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 235

Selecting Data Viewing Options. . . . . . . . . . . . . . . 236

Adding and Removing Baseline Points . . . . . . . . 240

Selecting the Graph X-axis Range . . . . . . . . . . . . . 241

Closing Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 243

Analysis Settings Overview. . . . . . . . . . . . . . . . . . . . . . . . . 244

Advanced Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . 246

Internal Standard Settings . . . . . . . . . . . . . . . . . . . . 246

Advanced Analysis Settings Groups . . . . . . . . . . . 247

Creating a New Analysis Group . . . . . . . . . . . . . . . 247

Changing the Default Analysis Group. . . . . . . . . 248

User Guide for Maurice, Maurice C. and Maurice S.

page 5

Modifying an Analysis Group . . . . . . . . . . . . . . . . . .249

Deleting an Analysis Group . . . . . . . . . . . . . . . . . . . .249

Applying Analysis Groups to Specific Run Data 250

Markers Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . . . .252

Markers Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .253

Changing the Injection Used for the CE-SDS MW

Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .253

Standards Analysis Settings Groups. . . . . . . . . . . .254

Creating a New Standards Group . . . . . . . . . . . . . .255

Changing the Default Standards Group . . . . . . .258

Modifying a Standards Group. . . . . . . . . . . . . . . . . .258

Deleting a Standards Group . . . . . . . . . . . . . . . . . . .258

Applying Standards Groups to Specific Run

Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259

Peak Fit Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . . . .261

Range Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262

Baseline Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263

Peak Find Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . .263

Peak Fit Analysis Settings Groups . . . . . . . . . . . . . .264

Creating a New Peak Fit Group. . . . . . . . . . . . . . . . .265

Changing the Default Peak Fit Group . . . . . . . . . .267

Modifying a Peak Fit Group . . . . . . . . . . . . . . . . . . . .267

Deleting a Peak Fit Group . . . . . . . . . . . . . . . . . . . . . .268

Applying Peak Fit Groups to Specific Run

Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .268

Peak Names Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271

Peak Names Analysis Settings Groups. . . . . . . . . .271

Creating a Peak Names Group . . . . . . . . . . . . . . . . .272

Modifying a Peak Names Group . . . . . . . . . . . . . . .275

Deleting a Peak Names Group . . . . . . . . . . . . . . . . .275

Applying Peak Names Groups to Run Data . . . .276

Injection Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 278

Example Analysis and Injection Report. . . . . . . . 280

Importing and Exporting Analysis Settings. . . . . . . . . 283

Importing Analysis Settings . . . . . . . . . . . . . . . . . . . 283

Exporting Analysis Settings. . . . . . . . . . . . . . . . . . . . 283

Chapter 12:

cIEF Data Analysis. . . . . . . . . . . . . . . . . . . . . . . . . 285

Analysis Screen Overview . . . . . . . . . . . . . . . . . . . . . . . . . . 286

Analysis Screen Panes . . . . . . . . . . . . . . . . . . . . . . . . . 286

Software Menus Active in the Analysis Screen . 287

Opening Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289

Opening One Run File. . . . . . . . . . . . . . . . . . . . . . . . . 289

Opening Multiple Run Files. . . . . . . . . . . . . . . . . . . . 289

How Run Data is Displayed . . . . . . . . . . . . . . . . . . . . . . . . 291

Experiment Pane: Batch Injection Information 291

Graph Pane: Electropherogram Data . . . . . . . . . 291

Peaks Pane: Calculated Results. . . . . . . . . . . . . . . . 292

Injections Pane: User-Specified Peak Names. . . 293

Viewing Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295

Switching Between Samples and Markers Data

Views. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295

Selecting and Displaying Injection Data . . . . . . 298

Switching Between Single and Multiple Views of

Injections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 301

Hiding Injection Data . . . . . . . . . . . . . . . . . . . . . . . . . 303

Data Notifications and Warnings. . . . . . . . . . . . . . . . . . 304

Checking Your Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 305

Group Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 305

Using Groups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 306

Viewing Sample Injection Groups . . . . . . . . . . . . . 306

Viewing Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 307

User Guide for Maurice, Maurice C. and Maurice S.

page 6

Hiding or Removing Injections in Group

Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .309

Copying Results Tables and Graphs. . . . . . . . . . . . . . . . .310

Copying Results Tables. . . . . . . . . . . . . . . . . . . . . . . . .310

Copying the Graph. . . . . . . . . . . . . . . . . . . . . . . . . . . . .310

Saving the Graph as an Image File. . . . . . . . . . . . .310

Exporting Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .311

Exporting Results Tables . . . . . . . . . . . . . . . . . . . . . . .311

Exporting Raw Sample Electropherogram

Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .311

Changing Sample Protein Identification. . . . . . . . . . . .312

Adding or Removing Sample Data. . . . . . . . . . . . .312

Hiding Sample Data . . . . . . . . . . . . . . . . . . . . . . . . . . .314

Changing Peak Names for Sample Data . . . . . . .315

Changing the Electropherogram View. . . . . . . . . . . . . .316

Autoscaling the Electropherogram . . . . . . . . . . . .317

Customizing the Data Display . . . . . . . . . . . . . . . . .317

Stacking Multiple Electropherograms. . . . . . . . . .323

Overlaying Multiple Electropherograms . . . . . . .324

Zooming. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .325

Selecting Data Viewing Options . . . . . . . . . . . . . . .326

Adding and Removing Baseline Points. . . . . . . . .330

Selecting the Graph X-axis Range . . . . . . . . . . . . . .331

Closing Run Files. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .333

Analysis Settings Overview. . . . . . . . . . . . . . . . . . . . . . . . . .334

Advanced Analysis Settings. . . . . . . . . . . . . . . . . . . . . . . . .336

pI Markers Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . .336

Advanced Analysis Settings Groups . . . . . . . . . . . .337

Creating a New Analysis Group . . . . . . . . . . . . . . . .337

Changing the Default Analysis Group. . . . . . . . . .338

Modifying an Analysis Group . . . . . . . . . . . . . . . . . .339

Deleting an Analysis Group . . . . . . . . . . . . . . . . . . . 339

Applying Analysis Groups to Specific Run Data 340

Detection Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 343

Changing the Detection Method. . . . . . . . . . . . . . 343

Changing the Detection Exposure . . . . . . . . . . . . 344

Peak Fit Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . . . 345

Range Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 346

Baseline Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 346

Peak Find Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 346

Peak Fit Analysis Settings Groups. . . . . . . . . . . . . . 348

Creating a New Peak Fit Group. . . . . . . . . . . . . . . . 348

Changing the Default Peak Fit Group . . . . . . . . . 350

Modifying a Peak Fit Group . . . . . . . . . . . . . . . . . . . 350

Deleting a Peak Fit Group . . . . . . . . . . . . . . . . . . . . . 351

Applying Peak Fit Groups to Specific Run Data 351

Peak Names Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 354

Peak Names Analysis Settings Groups. . . . . . . . . 354

Creating a Peak Names Group . . . . . . . . . . . . . . . . 355

Modifying a Peak Names Group . . . . . . . . . . . . . . 358

Deleting a Peak Names Group . . . . . . . . . . . . . . . . 358

Applying Peak Names Groups to Run Data. . . . 359

pI Markers Analysis Settings. . . . . . . . . . . . . . . . . . . . . . . . 362

Markers Analysis Settings Groups . . . . . . . . . . . . . 362

Creating a New Markers Group . . . . . . . . . . . . . . . 363

Changing the Default Markers Group . . . . . . . . . 365

Modifying a Markers Group . . . . . . . . . . . . . . . . . . . 366

Deleting a Markers Group . . . . . . . . . . . . . . . . . . . . . 366

Applying Markers Groups to Specific Run Data 367

Injection Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 369

Example Analysis and Injection Report . . . . . . . . 371

User Guide for Maurice, Maurice C. and Maurice S.

page 7

Importing and Exporting Analysis Settings . . . . . . . . .374

Importing Analysis Settings . . . . . . . . . . . . . . . . . . . .374

Exporting Analysis Settings . . . . . . . . . . . . . . . . . . . .374

Chapter 13:

Setting Your Preferences . . . . . . . . . . . . . . . . .377

Customize Your Preferences . . . . . . . . . . . . . . . . . . . . . . . .378

Enabling Access Control . . . . . . . . . . . . . . . . . . . . . . . . . . . .379

Setting Data Export Options . . . . . . . . . . . . . . . . . . . . . . . .379

Selecting Custom Plot Colors for Graph Overlay . . . .380

Grouping Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .382

Setting Up Maurice Systems to Send Tweets . . . . . . . .383

Chapter 14: Compass Access Control and

21 CFR Part 11 Compliance . . . . . . . . . . . . . .389

Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .390

Enabling Access Control . . . . . . . . . . . . . . . . . . . . . . . . . . . .391

Logging In to Compass for iCE . . . . . . . . . . . . . . . . . . . . . .392

Locking and Unlocking the Application. . . . . . . .393

Resolving Log In Issues . . . . . . . . . . . . . . . . . . . . . . . . .393

Saving Changes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .394

Password Policy Settings . . . . . . . . . . . . . . . . . . . . . . 406

LDAP Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 407

Encryption Details. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 407

Chapter 15:

Maintenance and Troubleshooting. . . . . 409

Cartridge Handling and Care . . . . . . . . . . . . . . . . . . . . . . 410

cIEF Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 410

CE-SDS Cartridge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 411

Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 412

Daily . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 412

Yearly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413

Changing the Fuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413

Spare Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 414

Software Updates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 414

Instrument Software (Embedded) Updates . . . . . . . . 415

Frequently Asked Questions: cIEF Applications. . . . . 415

Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 419

cIEF Application Troubleshooting . . . . . . . . . . . . . 419

CE-SDS Application Troubleshooting . . . . . . . . . 421

Signing Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .395

Instrument Command Log . . . . . . . . . . . . . . . . . . . . . . . . .395

Run File History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .398

Troubleshooting Problems and Suggested

Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .398

Authorization Server . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .399

Server Administration . . . . . . . . . . . . . . . . . . . . . . . . . .400

Adding Non-admin Users . . . . . . . . . . . . . . . . . . . . . .400

Adding Admin Users . . . . . . . . . . . . . . . . . . . . . . . . . . .404

Resetting User Passwords . . . . . . . . . . . . . . . . . . . . . .405

Audit Trail. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .406

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 16:

General Information . . . . . . . . . . . . . . . . . . . . . . 423

Compliance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424

Safety Guidelines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425

Chemical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425

Chemical Waste Hazards . . . . . . . . . . . . . . . . . . . . . 426

Waste Production and Disposal. . . . . . . . . . . . . . . 427

Safety Data Sheets . . . . . . . . . . . . . . . . . . . . . . . . . . . . 427

Instrument Safety Labels . . . . . . . . . . . . . . . . . . . . . . 428

Consumables and Reagents . . . . . . . . . . . . . . . . . . . . . . . 429

page 8

Maurice CE-SDS Consumables, Kits and

Reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .429

Maurice cIEF Consumables, Kits and Reagents .431

Maurice Systems Consumables and Reagents .433

Customer Service and Technical Support . . . . . . . . . . .433

Legal Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .435

Maurice Disclaimer of Warranty . . . . . . . . . . . . . . .435

Compass Software and Authorization Server

License Agreement. . . . . . . . . . . . . . . . . . . . . . . . . . . . .435

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 1:

Let’s Get Started

Chapter Overview

•Welcome

page 1

•Maurice Systems

User Guide for Maurice, Maurice C. and Maurice S.

Welc ome page 2

Welcome

Congratulations on bringing Maurice into your lab! We welcome you as a new user and are excited to be a part of your work. This user guide will provide you with details on system hardware, operating the system, how to use Compass for iCE software, maintenance procedures and other useful information.

To help you get the most from you new lab addition, we've added some attention phrases to guide you through the user guide:

NOTE Points out useful information.

IMPORTANT Indicates information necessary for proper operation of Maurice systems.

CAUTION Cautions you about potentially hazardous situations that could result in injury to you or

damage to the system.

!WARNING! Warns you that serious physical injury can result if the listed precautions aren’t followed.

Maurice Systems

Maurice, Maurice C. and Maurice S. systems give you identity, purity and heterogeneity data on your biologics, and get you to results faster with short development times and simple workflows!

• They're fluent in cIEF and CE-SDS. They take cIEF up a notch, and CE-SDS is a breeze. You'll get pI and charge heterogeneity data in less than 10 minutes flat — with the added bonus of same-time absorbance and native fluorescence for sensitivity down to 0.7 μg/mL. Their size applications have the high res and wide molecular weight range you need and they're done in 35 minutes.

• They make it easy. Just pop in a ready-to-go cartridge, drop in your sample vials or a 96-well plate, and hit start — they'll do the rest!

• They're time-savers. Develop methods fast so you get to results even faster. Your cIEF and CE-SDS methods are done in a day. The icing? You can develop platform methods and use them for multiple molecules. No maintenance and clean-up needed between the two applications.

• They're dependable. Get reproducible results with tight CVs day in and day out. Your data is reliable no matter what — across samples, users, instruments or labs.

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 2:

Getting Your Lab Ready

Chapter Overview

•Introduction

page 3

• Space Requirements

• Physical Specifications

• Electrical Requirements

• Environmental Requirements

• Software and Computer Requirements

• General Guidelines and Information

User Guide for Maurice, Maurice C. and Maurice S.

Introduction page 4

Introduction

This chapter will help you prepare the lab for Maurice. Please have the space, electrical and environmental requirements ready prior to scheduling your installation.

NOTE: Please wait for an authorized ProteinSimple Field Service Engineer to unpack and install Maurice for you. Don’t try doing this yourself. Handling Maurice incorrectly could cause injury to yourself or damage to the system.

Space Requirements

You need a lab bench or table that can support 100 lb (46 kg) and has enough space for both Maurice and his computer. There should be sufficient clearance for both heat ventilation and to provide access if Maurice needs service.

IMPORTANT

Maurice needs a stable surface and must remain level to work properly. The lab bench or table can’t shift or wobble under heavy weight. Don’t use anti-vibration tables either, since Maurice may not stay level while he’s working.

Dimension Meters Feet

Width 1.5 5.0

Depth 0.8 2.5

Height 0.5 1.5

Recommended space requirements for Maurice.

User Guide for Maurice, Maurice C. and Maurice S.

Physical Specifications page 5

Physical Specifications

Description Specification

Maurice’s Dimensions (Door Closed) 0.44 m x 0.42 m x 0.61m (H x W x D)

1.46’ x 1.38’ x 2.0’ (H x W x D)

Maurice’s Dimensions (Door Open) 0.44 m x 0.57 m x 0.61m (H x W x D)

1.46” x 2.43’ x 2.0’ (H x W x D)

Maurice’s Weight 46 kg (100 lb)

Computer Workstation Dimensions 0.41 m x 0.66 m x 0.76 m (H x W x D)

1.35' x 2.17' x 2.49' (H x W x D)

For indoor use only. Use up to altitudes of 1524 meters (5000 feet).

Table 2-1: Physical Specifications

Electrical Requirements

Maurice requires a dedicated, grounded circuit capable of delivering the appropriate current and voltage for your country. The power requirements for all three Maurice systems are 100 V- 240 V (AC), 50/60 Hz, 500 W.

In addition to these requirements, Maurice needs the grounded circuits terminate at the receptacles, and receptacles must be located within 10 ft (3 m) of the instrument.

Environmental Requirements

Maurice likes a consistent temperature in the lab (not too hot – not too cold). He works best when conditions stay within these ranges:

Requirement Specification

Operating temperature range 18 - 25 °C (64 - 77 °F)

Operating humidity range 20-80% relative, non-condensing

Table 2-2: Environmental requirements.

User Guide for Maurice, Maurice C. and Maurice S.

Software and Computer Requirements page 6

Software and Computer Requirements

Maurice brings his own computer to the lab with Compass for iCE software pre-installed. Compass for iCE is used to run cIEF and CE-SDS applications on Maurice and analyze resulting data. Just in case you need it, a CD containing Compass for iCE software also comes in the box. If you don't want to analyze your data at Maurice's workstation in the lab, Compass for iCE software can also be installed on a separate workstation, such as your desktop computer. Your computer must meet the recommended requirements listed below to run Compass for iCE software and process data.

Component Recommended

Operating System Windows 7

Processor Core i5

Memory 6 GB

Free Disk Space 100 GB

Ethernet Ports 2 - One is required to connect to Maurice, the other is

used for network access

USB Ports 2 - To connect the keyboard and mouse

Table 2-3: Computer requirements.

General Guidelines and Information

Intended Use

NOTE: Maurice is for research use only. Not for use in diagnostic procedures.

Lifting and Moving the System: Lift Maurice Correctly

IMPORTANT

Take all the standard precautions when lifting or moving Maurice. Since Maurice systems weigh 46 kg (100 lb), you should not lift him by yourself. Two people should lift him onto the lab bench.

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 3:

Maurice

Chapter Overview

•Maurice Systems

page 7

• External Components

• Internal Components

•Rear Panel

•Computer Workstation

User Guide for Maurice, Maurice C. and Maurice S.

Maurice Systems page 8

Maurice Systems

Maurice, Maurice C. and Maurice S. systems include the instrument, computer workstation, Compass for iCE software and cIEF or CE-SDS Cartridges.

Maurice with Computer Workstation

cIEF and CE-SDS Cartridges

All systems have the same hardware components, computer and software, the only difference between them are the applications you can run:

• Maurice: cIEF and CE-SDS applications

• Maurice C.: cIEF applications only

• Maurice S.: CE-SDS applications only

You can run samples in 96-well plates or in up to 48 sample vials with integrated 0.2 mL inserts on all three systems.

Maurice C. Maurice Maurice S.

User Guide for Maurice, Maurice C. and Maurice S.

External Components page 9

External Components

Tou ch Pla te t o Open Door

System Door

Status Light

!WARNING!

You can’t replace or service any parts on Maurice systems except for the power entry fuse.

System Door

Maurice's door gives you access to the inside of the instrument to load cartridges, reagents and samples. To open the door, first make sure the status light is a steady blue. Then just touch the metal touch plate on the top of the door to open it. Close it by pushing the door until you hear the latch engage.

NOTE: Maurice’s door must be closed before starting a batch.

User Guide for Maurice, Maurice C. and Maurice S.

Internal Components page 10

Status Light

The LED on Maurice’s front panel tells you what he’s doing. Here’s what his different status lights mean:

• Start-up (magenta): You've just turned on the power and Maurice is warming up.

• Ready (steady blue): Maurice is powered on and ready to go.

• Opening Door (long blue flash followed by blue pulses): Maurice’s door is opening.

• Running (pulsing blue): Maurice is running a batch.

• Trying to Open Door While Running (red flash): Maurice’s door can’t be opened when he’s running.

• Error (steady red): Maurice has detected an error. To get more information on the error, check the Status pane in the Run Summary Screen in Compass for iCE.

Internal Components

Cartridge Slot

The cartridge slot holds Maurice’s ready-to-go application cartridges. The cartridge it holds depends on the system:

• Maurice: cIEF and CE-SDS Cartridges

• Maurice C.: cIEF Cartridges only

• Maurice S.: CE-SDS Cartridges only

User Guide for Maurice, Maurice C. and Maurice S.

Internal Components page 11

Cartridge

Slot

The lights on either side of the cartridge slot will be orange after Maurice disengages the cartridge when the door is opened at the end of a batch, and whenever the slot is empty.

The lights change to

blue once a cartridge is installed correctly.

NOTE: You can find cartridge prep, installation and post-run procedures in Chapter 7, “Running cIEF Applications on Maurice and Maurice C.“ and Chapter 8, “Running CE-SDS Applications on Maurice and Maurice S.“

User Guide for Maurice, Maurice C. and Maurice S.

Internal Components page 12

Sample and Reagent Platform

Maurice’s sample and reagent platform has two rows for batch reagents. These reagents are kept at room temperature.

• Row P (top): These reagents are loaded under pressure during the batch. Only use glass reagent vials with pressure caps in this row. Use sure caps with CE-SDS reagents.

• Row N (bottom): Only use reagent vials with clear screw caps in this row.

blue pressure caps with cIEF reagents and orange pres-

The sample block holds either a 96-well plate or 48-vial metal insert and is temperature-controlled. You can set it to 4 °C, 10 °C, 15 °C or turn the temperature control off in Compass for iCE software.

Sample cooling turns on when the run starts, and takes a few minutes to reach the temperature setting. After a run, the sample block stays at the set temperature until you open Maurice’s door, then it shuts off until you start the next run. This prevents excess condensation.

NOTE: Because Maurice holds the sample block temperature after a run until you open the door, samples are still viable for your next run and after overnight runs.

User Guide for Maurice, Maurice C. and Maurice S.

Internal Components page 13

96-well Plate

Insert

NOTES: When you’re using a 96-well plate, well A1 should be in the top left corner of the insert.

48-vial

Insert

You can only use V-bottom plates with the 96-well plate insert.

Remove plate lids before inserting a 96-well plate into Maurice.

You can find info on where to load reagents and samples for cIEF applications in “Step 4: Load Samples and Reagents” on page 90 and for CE-SDS applications in “Step 4: Load Samples and Reagents” on page 124.

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Internal Components page 14

Rear Panel

Located on Maurice’s rear panel is the power entry, power switch and network connector.

Power

Entry

Fuse

Power

Switch

Network Connector

• System Power - The main system power components consist of the power input, fuse and power switch

!WARNING!

Only use the power supply cord provided with Maurice. If the cord is damaged, please contact ProteinSimple Technical Support.

!WARNING! You can't replace or service any parts on Maurice except the power entry fuse.

!WARNING! SHOCK HAZARD

Disconnect the power cord from Maurice’s power input to disconnect power to the instrument.

• Network connection - A 10/100/1000 Mbps Ethernet (RJ-45 connector) is used to connect Maurice to a computer or local network.

NOTE: Serial numbers are used to identify individual instruments.

System Labels

A full system label is located on the rear panel. It includes the ProteinSimple location, system model, power requirements, serial number and certification markings.

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Internal Components page 15

A serial number label is located on the Maurice system’s front lower right side, on the silver system base.

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Computer Workstation page 16

Computer Workstation

The PC has two built-in Ethernet ports, one is used for Maurice and the other is available for your company’s network. ProteinSimple configures one port to have a fixed IP for a local link connection to the instrument, the other is configurable by users and will typically use a DHCP for dynamic IP.

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 4:

Compass for iCE Overview

Chapter Overview

• Launching Compass for iCE

• Compass for iCE Overview

page 17

•Software Menus

• Changing the Compass for iCE Main Window Layout

• Software Help

• Checking for and Installing New Versions of Compass for iCE

•Viewing Release Notes

• Viewing the Software Log

• Compass for iCE Version Information

• Directory and File Information

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page 18 Chapter 4: Compass for iCE Overview

Instrument

Status Bar

Screen Panes

Title bar

Screen Tabs

Status Bar

Main Menu

Launching Compass for iCE

To open Compass for iCE, just double-click the icon on the computer desktop.

Compass for iCE Overview

Compass for iCE has three main screens:

• Batch - You’ll create and review your batch.

• Run Summary - Check out the status of your run.

• Analysis - Take a look at the data from your experiment.

Each screen has these components:

User Guide for Maurice, Maurice C. and Maurice S.

Compass for iCE Overview page 19

Changing the Screen View

To toggle between the Batch, Run Summary and Analysis screens, just click the button in the screen tab located in the upper right corner of the main window.

Batch Screen

The Batch screen is used to create, view, and edit batches. You can assign samples to 96-well plate wells or vials, create and modify methods, customize your injection list and assign methods to each of your injections.

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page 20 Chapter 4: Compass for iCE Overview

Run Summary Screen

The Run Summary screen is used to monitor status of a batch in progress, the CE-SDS separation or cIEF Focus series for each injection and the current and voltage plots for each injection.

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Compass for iCE Overview page 21

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page 22 Chapter 4: Compass for iCE Overview

Analysis Screen

The Analysis screen is used to view data from your batch, including the graph view (electropherograms) and a table with your results. You can also analyze your data for completed runs.

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Compass for iCE Overview page 23

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page 24 Chapter 4: Compass for iCE Overview

Screen Panes

Each of the Batch, Run Summary and Analysis screens have multiple panes that let you view the individual components of a batch, method or data file. Each pane has a labeled tab and a unique icon. We'll describe panes specific to each screen later in the individual screen sections.

The active pane in a screen is blue. To view a pane, click in the pane or on its tab. The example below shows panes in the Batch screen, and the Graph pane is active:

Title Bar

In the title bar you will see the batch file name and the icons that allow the main Compass for iCE window to be minimized, maximized or closed.

Main Menu

Access to various software, instrument and screen operations is available through the main menu. More details on menu commands can be found in “Software Menus” on page 25.

Instrument Status Bar

The instrument status bar is used to start batches and cleaning protocols, indicate system status and show run progress. More details on instrument control and status can be found in Chapter 10, “Controlling Maurice, Maurice C. and Maurice S.“.

User Guide for Maurice, Maurice C. and Maurice S.

Software Menus page 25

NOTE: You will only see the instrument status bar when Compass for iCE is connected to an instrument. There is no status bar on computer workstations that you're only using for data analysis.

Screen Tab

The screen tab lets you move between Batch, Run Summary or Analysis screens and is located in the upper right corner of the main window. Just click a button to view a screen.

View Bar

The view bar is only displayed in the Analysis screen as part of the main menu, and allows you to switch between viewing standards or sample data, data for a single injection or all injections in the batch, or grouped injection data. View bar options are in “Viewing Run Data” on page 295 for cIEF applications or page 205 for CE-SDS applications.

Compass for iCE Status Bar

The status bar is in the lower right corner of the main window. It displays active software processes and their progress.

Software Menus

Some of the items in the Compass for iCE main menu are available in specific screens only, and menu commands change depending on which screen is active. You can find menus and commands available for each screen in the Chapter 5, “cIEF Batches“, Chapter 6, “CE-SDS Batches“, Chapter 9, “Run Status“, Chapter 12, “cI EF Data Analysis“ and Chapter 11, “CE-SDS Data Analysis“.

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page 26 Chapter 4: Compass for iCE Overview

File Menu

The File menu contains basic file commands.

Edit Menu

The Edit menu contains basic editing commands, analysis and preferences options. Specific details on preferences are described in Chapter 13, “Setting Your Preferences“.

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Software Menus page 27

View Menu

The View menu is only available in the Analysis screen, and allows you to change how your data is displayed. For more info on view options check out “Viewing Run Data” on page 295 for cIEF applications or page 205 for CE-SDS applications, and “Using Groups” on page 306 for cIEF applications or page 216 for CE-SDS applications.

Instrument Menu

The Instrument menu is only available when the software is connected directly to your instrument. You can lean more about instrument control options in Chapter 10, “Controlling Maurice, Maurice C. and Maurice S.“

Window Menu

The Window menu lets you to switch between the Batch, Run Summary or Analysis screens, and restore screens to the default layout.

• Batch - Displays the Batch screen where you create, view, and edit batches.

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page 28 Chapter 4: Compass for iCE Overview

• Run Summary - Displays the Run Summary screen which lets you view the status of a batch in progress.

• Analysis - Displays the Analysis screen that lets you view electropherograms and results and change analysis parameters

• Default Layout - Restores the individual panes in the current screen back to their default size and location.

Help Menu

The Help menu gives you access to Help, software updates, release notes and other software info.

• User Guide - Displays the User Guide for Maurice, Maurice C. and Maurice S.

• Check for Updates - Automatically checks to see if a new version of Compass for iCE is available.

• Release Notes - Displays the software release notes for the current and prior versions.

• Compass for iCE Log - Displays the software log file.

• About Compass for iCE - Displays the software version and build information.

Changing the Compass for iCE Main Window Layout

You can easily resize the main window and the individual panes in each screen. Screen panes can also be moved outside of the main window.

Resizing the Main Compass for iCE Window

To resize the main window, roll the mouse over a corner or border until the sizing arrow appears. Then just click and drag to resize.

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Changing the Compass for iCE Main Window Layout page 29

Resizing the Screen Tab

The screen tab can be sized to show all or just some of the screen buttons. To resize, roll the mouse over the left edge of the tab until the sizing arrow appears, then click and drag to resize. If a screen button is hidden, a double arrow will display in the tab. Just click to display and select the hidden screen.

Resizing Screen Panes

• To re size a pane - Roll the mouse over the pane border until the sizing arrow appears. Then just click and drag to resize.

• To maximize a pane - Click the maximize button in the upper right corner or double-click the tab.

The other panes in the screen will automatically minimize to pane bars in the task area along the window border.

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page 30 Chapter 4: Compass for iCE Overview

• To restore all minimized panes - Click Restore on the minimized pane bar.

• To restore only one minimized pane - Click the pane icon on the minimized pane bar.

• To restore a maximized pane to its original size - Double-click the tab or right click the tab and click

Restore.

• To restore all panes to their original sizes - Select Window in the main menu and click Default

Layout.

Changing the Location of Screen Panes

Panes can be moved to different locations within a screen.

• To move a pane - Click on its tab and drag it to the new location. As the pane is moved, area guides will display to assist you in choosing a drop location.

Area guides with a black arrow let you know that if the pane is dropped at that location, it will be resized and relocated as an individual pane in that area of the screen.

Area guides with a folder let you know that if the pane is dropped at that location, it will be added as a new tab in an area with one or more pane tabs.

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Changing the Compass for iCE Main Window Layout page 31

Area guides with a window let you know that if the pane is dropped at that location, it will be a separate window outside the Compass for iCE main window.

This example shows the Analysis screen after moving the Graph pane:

• To detach a pane from the main window - Click on its tab and drag it outside the main Compass for iCE window or right click the tab and click Detached.

• To move a detached pane back inside the main window - Right click the tab and deselect

Detached.

• To restore all panes to their original locations - Select

Window in the main menu and click

Default Layout.

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page 32 Chapter 4: Compass for iCE Overview

Restoring the Main Window to the Default Layout

To restore screen pane sizes and locations to the original Compass for iCE layout, select main menu and click Default Layout.

Window from the

Software Help

Help and click User Guide to view Maurice Systems User Guide.

Select

Checking for and Installing New Versions of Compass for iCE

The software can automatically check to see if a newer version of software is available. To do this:

1. Make sure the computer being used has an active internet connection.

2. Select

3. Click

4. Follow the on-screen instructions to complete the software installation.

5. Reboot the computer before using the new version of software.

Help and click Check for Updates. If an update is found, a screen will display with the new ver-

sion that’s available.

Finish to start the download and install the update.

Viewing Release Notes

Select Help and click Release Notes to view a PDF with feature updates and bug fixes for new and past ver- sions of Compass for iCE. We recommend you review these notes whenever a software update is installed.

NOTE: You can contact ProteinSimple Technical Support to request the release notes for new versions of Compass for iCE before you install it.

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Viewing the Software Log page 33

Viewing the Software Log

Help and click Compass Log to view the software log file.

Select

Compass for iCE Version Information

Help and click About Compass for iCE to view the software version and build number information.

Select

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page 34 Chapter 4: Compass for iCE Overview

Directory and File Information

The main Compass for iCE directory is located in the Program Files folder, and also contains PDF files of the User Guide for Maurice, Maurice C. and Maurice S.

Batch and run files are located in the Documents folder in the User directory on your computer:

• Batches Folder - Contains all batch files that you’ve saved.

• New Batches Folder - Contains Maurice batch template files.

• Runs Folder - Contains all batch data files. Data is automatically written to this folder.

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Directory and File Information page 35

NOTE: When a Compass for iCE software update is performed, the template s in the New Batch folder are overwritten. If you have customized these batches, we recommend saving them in a unique subfolder prior to updating the software, then transferring them back to the New Batch folder after the update to avoid losing your customizations.

File Types

These file types are used by Compass for iCE:

• Batch Files - Use a *.batch file extension.

• Run Files - Use a *.mbz file extension. The default file format for run files is Date_Time_BatchName. An example run file name would be 2016-01-28_18-50-53_CE-SDS.mbz.

• Analysis Settings Files - Exported analysis settings files use a *.settings file extension.

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page 36 Chapter 4: Compass for iCE Overview

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 5:

cIEF Batches

Chapter Overview

• Batch Screen Overview

• Opening a Batch

page 37

• Creating a New Batch

• Viewing Replicate Injections

•Batch History

• Making Changes to a Batch

• Viewing and Editing Batches in Completed Runs

•Batch Reports

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page 38 Chapter 5: cIEF Batches

Batch Screen Overview

You can use the Batch screen to create, view, and edit batches. To get to this screen, click the tab:

Batch Screen Panes

The Batch screen has five panes:

• Layout - Displays a map of either the 96-well plate or 48-vial tray of batch sample locations.

• Injections - Lists the injections, sample ID, sample locations and methods that Maurice or Maurice C. will execute for each sample in the batch.

• History - Lists all batch file events from initial creation to the most current update.

• Notes - Lets you enter specific information about your batch.

• Methods - Lets you create methods and enter method parameters used in the batch.

Batch screen

User Guide for Maurice, Maurice C. and Maurice S.

Batch Screen Overview page 39

Software Menus Active in the Batch Screen

These main menu items are active in the Batch screen:

•File

•Edit

• Instrument (when the software is connected to Maurice or Maurice S.)

•Window

•Help

File Menu

These File menu options are active:

• New Batch - Creates a new batch from a starter template.

• Open Batch - Opens an existing batch.

• Save/Save As - Saves the open batch.

• Batch Report - Exports a table of sample and method details for each injection in the batch as a PDF file.

• Exit - Closes Compass for iCE.

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page 40 Chapter 5: cIEF Batches

Edit Menu

The following Edit menu options are active in the Batch screen:

• Cut - Cuts the information currently selected.

• Copy - Copies the information currently selected.

• Paste - Pastes the copied information.

NOTE: Cut, Copy and Paste work differently depending on the pane you’re in. How and when you can use them is described throughout the chapter.

• Plate Layout - Lets you select between a 96-well plate or a 48-vial tray to run samples in.

• Default Analysis - Displays the default settings that will be used to analyze the data generated with your batch.

• Preferences - Lets you set and save your preferences for data export, graph colors, grouped data and Twitter settings. See Chapter 13, “Setting Your Preferences“ for more information.

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Opening a Batch page 41

Opening a Batch

To open an existing batch:

1. Select

File in the main menu and click Open Batch.

2. A list of the last five batches opened will display. Select one of those or click Browse to open the Batches folder and select a different one.

3. To make changes to the batch, see the steps in “Creating a New Batch” on page 42. When you’re done, select

File from the main menu and click Save.

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page 42 Chapter 5: cIEF Batches

Creating a New Batch

To create a new batch, we recommend that you use one of the template methods and make any necessary modifications from there.

Step 1 - Open a Template Batch

1. Select File in the main menu and click New Batch:

NOTE: If you’re using a Maurice system, both cIEF and CE-SDS template batches are available in the menu.

2. Select

Maurice cIEF. A batch using the default method will display.

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Creating a New Batch page 43

Step 2 - Assign Your Samples

The Layout pane shows the default locations of where batch reagent should be placed in your Maurice system’s samples and reagents platform, and a map of either the 96-well plate or the 48-vial tray used for samples.

The same reagent locations are used for every batch:

• P1 - 0.5% Methyl Cellulose with

blue pressure cap

• P2 - Fluorescence Calibration Standard with blue pressure cap

• P3 - Water vial with blue pressure cap

• P6 - Empty vial (air) with blue pressure cap

• N1 - Water vial with clear screw cap

1. To assign samples, select 48 vials or a 96-well plate depending on what you’re running. Clicking on the vial/plate icon toggles between formats.

2. To select samples:

• Add samples and select methods later: Use your mouse to highlight the well or vial positions

your samples are located in, then click

Add. For this example we’re using vials.

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page 44 Chapter 5: cIEF Batches

NOTE: The template batch automatically adds a sample in well or vial A1 by default, but if you don’t have a sample in this position you can remove it after you’ve added new positions for your samples.

• Add samples with preassigned methods: Highlight the well or vial positions your samples are located in, then right-click and select a method.

Either option of adding samples populates the Injections table:

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Creating a New Batch page 45

To remove samples, just highlight the ones you want to remove and click

Remove. They’ll also be

removed in the Injections table. You can also right-click well(s) or vial(s) in the Layout pane and click

Remove Sample(s).

3. The sample platform is temperature controlled with a default setting of 10 °C. To change the temperature or turn it off, click the

down arrow next to the temperature and select an option.

NOTE: The two reagent rows are kept at room temperature.

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page 46 Chapter 5: cIEF Batches

Step 3 - Assign Your Method Parameters

NOTE: We recommend using the default method parameters. Please see the Maurice cIEF Method Development Guide for more information on method optimization.

The Methods pane lists all of the methods used in a batch. You can add and remove methods here and change method parameters.

1. In the Methods table, click the first cell in the Name column and enter a new method name if needed.

2. Click the first cell in the Separation column, then click the selection button

[...] to set the pre-focusing

and focusing time (in minutes) and voltage (V ).

• To change time and voltage parameters: Just click in a cell under Time or Voltage and type the new value(s).

• To add a profile step: Click

Add. A new row will be added in the table. Then just type in a load

time (in minutes) and voltage value (in V).

• To remove a profile step: Select the row you want to remove and click

Remove.

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Creating a New Batch page 47

3. Click the first cell in the Detection column the selection button [...] to set your exposure times for absorption and fluorescence detection modes.

• To change the exposure time: Just click in a cell under

Exposure and type the new value(s) in

seconds.

NOTES: The first exposure is an instrument default setting and can’t be changed.

Fluorescence is the default detection for the remaining exposures and can’t be changed.

• To add a profile step: Click

Add. A new row will be added in the table. Then just type in an expo-

sure time (in seconds).

• To remove a profile step: Select the row you want to remove and click

Remove.

4. Click the first cell in the Sample Load(s) column and set the load time in seconds.

NOTE: We recommend using the default Sample Load time of 55 seconds. Please contact ProteinSimple Technical Support if you have questions on the Sample Load time to use for your application.

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page 48 Chapter 5: cIEF Batches

5. Click the first cell in the pI Markers column to select pI markers. Add new markers or remove existing ones then click

OK.

NOTE: When you edit the pI markers in the method for a batch, Compass for iCE automatically creates a Markers group in the pI Markers Analysis settings for you.

• To add a pI marker: Click

Add. A new row will be added in the table. Then just type in a pI and a

position (in pixels).

• To remove a pI marker: Select the row you want to remove and click

Remove.

6. Optional: Click the first cell in the Ampholytes column and enter the ampholytes you’re using.

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Creating a New Batch page 49

7. Optional: Click the first cell in the Additives column and enter any additives you’re using.

8. You can now:

•Click

New in the upper right corner of the Methods pane to add a new one if you want to use dif-

ferent methods for different samples. Then just repeat the steps above for the new method.

• Make updates to the remaining methods by repeating the prior steps.

• Select a method and click

Remove in the upper right corner of the Methods pane to delete it.

Step 4 - Set Up Your Injections

The Injection pane lists all sample injections for the batch. Any samples that were added in “Step 2 - Assign Your Samples” are automatically added to this list.

Selecting an injection in the table also selects the sample the injection will be made from in the plate or vial map in the Layout pane and vice-versa.

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1. To add sample names, click the

Sample ID cell for the injection and type a name.

The sample name also displays when you hover the mouse over the sample in the plate or vial map:

2. Assign your methods to each injection. Just click the

Method cell for the injection and select a method

from the drop down menu. If you added your samples with pre-assigned methods and don’t need to make any changes, skip to the next step.

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Creating a New Batch page 51

Hovering over a method name displays the method parameters:

3. You can add or remove injections as needed using a few different options. If you don’t need to make any changes here, just skip to the next step.

• To add sequential replicate injections: Highlight an injection and click

Replicate. A new injec-

tion will be added under the row you selected

• To add injections at the end of the batch: Click on any sample position in the Layout pane or

sample injection in the Injection table and click

Add. A new injection using the same sample will

be added to the end of the table. Assign the method if needed.

• To copy and paste injections: Right-click on an injection in the Injection table and select

Click on the injection number above where you want to insert the copied injection, right click and select

Paste. The copied injection will be inserted under the row you selected.

Step 5 - Add Batch Notes (Optional)

1. Click on the

2. Click in the notes area and type any information you want to add about your batch.

User Guide for Maurice, Maurice C. and Maurice S.

Notes pane.

Copy.

page 52 Chapter 5: cIEF Batches

Step 6 - Modify Default Analysis Parameters (Optional)

You can preset the parameters used to analyze data generated with the batch. We recommend using the default parameters for cIEF applications, but if you need to modify parameters:

1. Select

Edit from the main menu and click Default Analysis. The following screen will display:

2. Change the parameters you want to, then click OK. For detailed information on analysis parameters, please refer to “Analysis Settings Overview” on page 334.

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Viewing Replicate Injections page 53

Step 7 - Save Your Batch

1. Once all of your sample, method and injection info is entered, select on the batch if you want, then click Save.

2. Enter a name for your batch then click

Save.

File > Save. Enter any comments

Viewing Replicate Injections

Injections with replicates have an arrow next to the injection number. You can expand or collapse the list of replicate injections by toggling the arrow:

• To show all replicate injections in the batch, click the

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Expand All Injections button.

page 54 Chapter 5: cIEF Batches

• To hide all replicate injections in the batch, click the

Collapse All Injections button.

Batch History

Clicking on the History pane shows the batch event history, starting with the date and time the batch was created through the most current event. Clicking on a row in the table displays the full event details in the box under the table.

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Making Changes to a Batch page 55

• Date: Date and time of the batch event.

• User Name: User that initiated the event. User names only display if you’re using the Access Con-

trol feature to help satisfy the 21CFR Part 11 data security requirements. Go to “Enabling Access Control” on page 391 to learn how to set it up.

• Message: Description of the event that took place.

• Comment: Comments entered by the user when the batch was saved.

You can copy the information in the History pane to use in other documents:

1. Click the

2. Click

3. Open a document and click

History pane to make sure it’s active.

Edit in the main menu and select Copy.

Paste.

Making Changes to a Batch

1. Select

User Guide for Maurice, Maurice C. and Maurice S.

File in the main menu and click Open Batch.

page 56 Chapter 5: cIEF Batches

2. A list of the last five batches opened will display.

• Select one of these files or click

• To open a batch used in a run file, click

Browse to open the Batch folder and select a different one.

Browse. Go to the Run folder in the Compass for iCE direc-

tory and select the run file that uses the batch you want to edit.

3. To make changes to the batch, see the steps in “Creating a New Batch” on page 42. Then select the main menu and click Save or Save As.

Viewing and Editing Batches in Completed Runs

1. Click the

2. After the run opens, click the

Analysis screen and open your run file(s).

Batch screen to view the batch used with the run.

File from

If you opened more than one run file, you can switch between viewing batches for each file. Just click the

down arrow in the Run box and select the batch you want to view from the drop down list.

3. In completed run files, you can only make edits to the sample and method names in a batch, and these changes are saved to the run file only. Update sample and method names as needed.

4. Click the

Analysis screen. Then select File from the main menu and click Save or Save As to save the

new changes to the run file.

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Batch Reports page 57

Batch Reports

You can export a PDF file of sample and method details for each injection in the batch for completed run files.

1. Go to the

Analysis or Run Summary screen, then click File > Open Run and select a run file (if you

don’t have one open already).

2. After the run opens, go to the

3. Select

File from the main menu and click Batch Report.

Batch screen.

4. The report name defaults to the run file name. If you want to change it, type in the Report Name box to make updates.

5. The Batch Report PDF is exported to the Runs folder in the Compass for iCE directory. It’ll be in a folder with the report name used in the prior step. When the reports are done, the folder opens for you automatically.

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Here’s an example Batch Report:

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 6:

CE-SDS Batches

Chapter Overview

• Batch Screen Overview

• Opening a Batch

page 59

• Creating a New Batch

• Viewing Replicate Injections

•Batch History

• Making Changes to a Batch

• Viewing and Editing Batches in Completed Runs

•Batch Reports

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page 60 Chapter 6: CE-SDS Batches

Batch Screen Overview

You can use the Batch screen to create, view, and edit batches. To get to this screen, click the tab:

Batch Screen Panes

The Batch screen has five panes:

• Layout - Displays a map of either the 96-well plate or 48-vial tray of batch sample locations.

• Injections - Lists the injections, sample ID, sample locations and methods that Maurice or Maurice S. will execute for each sample in the batch.

• History - Lists all batch file events from initial creation to the most current update.

• Notes - Lets you enter specific information about your batch.

• Methods - Lets you create methods and enter method parameters used in the batch.

Batch screen

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Batch Screen Overview page 61

Software Menus Active in the Batch Screen

These main menu items are active in the Batch screen:

•File

•Edit

• Instrument (when the software is connected to Maurice or Maurice S.)

•Window

•Help

File Menu

These File menu options are active:

• New Batch - Creates a new batch from a starter template.

• Open Batch - Opens an existing batch.

• Save/Save As - Saves the open batch.

• Batch Report - Exports a table of sample and method details for each injection in the batch as a PDF file. This menu item is only active for batches in completed runs.

• Exit - Closes Compass for iCE.

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Edit Menu

The following Edit menu options are active in the Batch screen:

• Cut - Cuts the information currently selected.

• Copy - Copies the information currently selected.

• Paste - Pastes the copied information.

NOTE: Cut, Copy and Paste work differently depending on the pane you’re in. How and when you can use them is described throughout the chapter.

• Plate Layout - Lets you select between a 96-well plate or a 48-vial tray to run samples in.

• Default Analysis - Displays the default settings that will be used to analyze the data generated with your batch.

• Preferences - Lets you set and save your preferences for data export, graph colors, grouped data and Twitter settings. See Chapter 13, “Setting Your Preferences“ for more information.

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Opening a Batch page 63

Opening a Batch

To open an existing batch:

1. Select

File in the main menu and click Open Batch.

2. A list of the last five batches opened will display. Select one of those or click Browse to open the Batches folder and select a different one.

3. To make changes to the batch, see the steps in “Creating a New Batch” on page 64. When you’re done, select

File from the main menu and click Save.

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page 64 Chapter 6: CE-SDS Batches

Creating a New Batch

To create a new batch, we recommend that you use one of the template methods and make any necessary modifications from there.

Step 1 - Open a Template Batch

1. Select File in the main menu and click New Batch:

NOTE: If you’re using a Maurice system, both cIEF and CE-SDS template batches are available in the menu.

2. Select

Maurice CE-SDS. A batch using the default method will display.

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Creating a New Batch page 65

Step 2 - Assign Your Samples

The same reagent locations are used for every batch:

• P1 - Conditioning Solution 1 with

orange pressure cap

• P2 - Conditioning Solution 2 with orange pressure cap

• P3 - DI water with orange pressure cap

• P4 - Separation Matrix with orange pressure cap

• P5 - Wash Solution vial with orange pressure cap

• P6 - Empty vial (air) with orange pressure cap

• N1 - Wash Solution vial with clear screw cap

• N2 - Wash Solution vial with clear screw cap

• N4 - Running Buffer - Bottom with clear screw cap

1. To assign samples, select a 96-well plate or 48 vials depending on what you’re running. Clicking on the vial/plate icon toggles between formats.

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page 66 Chapter 6: CE-SDS Batches

2. To select samples:

• Add samples and select methods later: Use your mouse to highlight the well or vial positions your samples are located in, then click

Add. For this example we’re using a 96-well plate.

• Add samples with preassigned methods: Highlight the well or vial positions your samples are located in, then right-click and select a method.

Either option of adding samples populates the Injections table:

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Creating a New Batch page 67

To remove samples, just highlight the ones you want to remove and click

Remove. They’ll also be

removed in the Injections table. You can also right-click well(s) or vial(s) in the Layout pane and click

Remove Sample(s).

3. Compass for iCE can monitor the current during a separation for you, stop it if the current drops below the minimum value and reinject the sample for you automatically. Reinjections are enabled by default, but if you don’t want to use this feature just click the reinject icon to toggle it off.

NOTES: If a sample was reinjected, the injection row will be flagged in the Injections pane in the Run Summary screen. See “Injection Flags” on page 158 for more info.

A maximum of 10 reinjections are allowed per batch.

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page 68 Chapter 6: CE-SDS Batches

4. The sample platform is temperature controlled with a default setting of 10 °C. To change the temperature or turn it off, click the down arrow next to the temperature and select an option.

NOTE: The two reagent rows are kept at room temperature.

Step 3 - Assign Your Method Parameters

NOTE: There are three default methods. We recommend using the default method parameters for the listed sample types. Please see the Maurice CE-SDS Application Guide for more information on method optimization.

The Methods pane lists all of the methods used in a batch. You can add and remove methods here and change method parameters.

1. In the Methods table, click the first cell in the Name column and enter a new method name if needed.

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Creating a New Batch page 69

2. Click the first cell in the Sample Load column, then click the selection button [...] to set your sample load profile time (in seconds) and voltage.

• To change time and voltage parameters: Just click in a cell under Time or Voltage and type

the new value(s).

• To add a profile step: Click

Add. A new row will be added in the table. Then just type in a load

time (in seconds) and voltage value.

• To remove a profile step: Select the row you want to remove and click

3. Click the first cell in the Separation column the selection button

[...] to set your separation profile

Remove.

parameters (in minutes) and voltage.

NOTE: Run your reduced IgG samples and IgG Standard for 25 minutes and the CE-SDS MW Markers for 30 minutes. Run your non-reduced IgG samples and IgG Standard for 35 minutes. The default separation voltage for all sample types is 5750 volts.

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page 70 Chapter 6: CE-SDS Batches

• To change time and voltage parameters: Just click in a cell under

Time or Voltage and type

the new value(s).

• To add a profile step: Click

Add. A new row will be added in the table. Then just type in a load

time (in seconds) and voltage value.

• To remove a profile step: Select the row you want to remove and click

Remove.

4. You can now:

•Click

New in the upper right corner of the Methods pane to add a new one if you want to use dif-

ferent methods for different samples. Then just repeat the steps above for the new method.

• Make updates to the remaining methods by repeating the prior steps.

• Select a method and click

Remove in the upper right corner of the Methods pane to delete it.

Step 4 - Set Up Your Injections

The Injection pane lists all sample injections for the batch. Any samples that were added in “Step 2 - Assign Your Samples” are automatically added to this list.

Selecting an injection in the table also selects the sample the injection will be made from in the plate or vial map in the Layout pane and vice-versa.

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Creating a New Batch page 71

1. To add sample names, click the

Sample ID cell for the injection and type a name.

The sample name also displays when you hover the mouse over the sample in the plate or vial map:

2. Assign your methods to each injection. Just click the Method cell for the injection and select a method from the drop down menu. If you added your samples with pre-assigned methods and don’t need to make any changes, skip to the next step.

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page 72 Chapter 6: CE-SDS Batches

Hovering over a method name displays the method parameters:

3. You can add or remove injections as needed using a few different options. If you don’t need to make any changes here, just skip to the next step.

• To add sequential replicate injections: Highlight an injection and click

Replicate. A new injec-

tion will be added under the row you selected

• To add injections at the end of the batch: Click on any sample position in the Layout pane or

sample injection in the Injection table and click

Add. A new injection using the same sample will

be added to the end of the table. Assign the method if needed.

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Creating a New Batch page 73

• To copy and paste injections: Right-click on an injection in the Injection table and select Copy. Click on the injection number above where you want to insert the copied injection, right click and select

Paste. The copied injection will be inserted under the row you selected.

Step 5 - Add Batch Notes (Optional)

1. Click on the

Notes pane.

2. Click in the notes area and type any information you want to add about your batch.

Step 6 - Modify Default Analysis Parameters (Optional)

You can preset the parameters used to analyze data generated with the batch. We recommend using the default parameters for CE-SDS applications, but if you need to modify parameters:

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page 74 Chapter 6: CE-SDS Batches

1. Select Edit from the main menu and click Default Analysis. The following screen will display:

2. Change the parameters you want to, then click OK. For detailed information on analysis parameters, please refer to “Analysis Settings Overview” on page 244.

Step 7 - Save Your Batch

1. Once all of your sample, method and injection info is entered, select on the batch if you want, then click Save.

User Guide for Maurice, Maurice C. and Maurice S.

File > Save. Enter any comments

Viewing Replicate Injections page 75

2. Enter a name for your batch then click

Save.

Viewing Replicate Injections

Injections with replicates have an arrow next to the injection number. You can expand or collapse the list of replicate injections by toggling the arrow:

• To show all replicate injections in the batch, click the

Expand All Injections button.

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page 76 Chapter 6: CE-SDS Batches

• To hide all replicate injections in the batch, click the

Collapse All Injections button.

Batch History

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Making Changes to a Batch page 77

• Date: Date and time of the batch event.

• User Name: User that initiated the event. User names only display if you’re using the Access Control feature to help satisfy the 21CFR Part 11 data security requirements. Go to “Enabling Access Control” on page 391 to learn how to set it up.

• Message: Description of the event that took place.

• Comment: Comments entered by the user when the batch was saved.

You can copy the information in the History pane to use in other documents:

1. Click the

2. Click

3. Open a document and click

History pane to make sure it’s active.

Edit in the main menu and select Copy.

Paste.

Making Changes to a Batch

1. Select

User Guide for Maurice, Maurice C. and Maurice S.

File in the main menu and click Open Batch.

page 78 Chapter 6: CE-SDS Batches

2. A list of the last five batches opened will display.

• Select one of these files or click

• To open a batch used in a run file, click

Browse to open the Batch folder and select a different one.

Browse. Go to the Run folder in the Compass for iCE direc-

tory and select the run file that uses the batch you want to edit.

3. To make changes to the batch, see the steps in “Creating a New Batch” on page 64. Then select the main menu and click Save or Save As.

Viewing and Editing Batches in Completed Runs

1. Click the

2. After the run opens, click the

Analysis screen and open your run file(s).

Batch screen to view the batch used with the run.

File from

If you opened more than one run file, you can switch between viewing batches for each file. Just click the

down arrow in the Run box and select the batch you want to view from the drop down list.

3. In completed run files, you can only make edits to the sample and method names in a batch, and these changes are saved to the run file only. Update sample and method names as needed.

4. Click the

Analysis screen. Then select File from the main menu and click Save or Save As to save the

new changes to the run file.

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Batch Reports page 79

Batch Reports

You can export a PDF file of sample and method details for each injection in the batch for completed run files.

1. Go to the

Analysis or Run Summary screen, then click File > Open Run and select a run file (if you

don’t have one open already).

2. After the run opens, go to the

3. Select

File from the main menu and click Batch Report.

Batch screen.

4. The report name defaults to the run file name. If you want to change it, type in the Report Name box to make updates.

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page 80 Chapter 6: CE-SDS Batches

Here’s an example Batch Report:

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Chapter 7:

Running cIEF Applications on Maurice and Maurice C.

page 81

Chapter Overview

• Before You Throw the Switch

•Power Up

• Running cIEF Applications

• Post-batch Procedures

•Checking Your Data

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Before You Throw the Switch page 82

Before You Throw the Switch

Ensure that everyone using Maurice have:

• Received instruction in general safety practices for laboratories.

• Received instruction in specific safety practices for Maurice.

• Received instruction on handling of biohazards (if biohazardous materials are to be used on Maurice).

• Read and understood all Product Inserts and related Safety Data Sheets (SDS).

Power Up

1. Turn on the computer connected to Maurice.

2. Turn on Maurice’s main power switch.

3. Wait for Maurice to initialize. His indicator light will turn from magenta to blue when he’s done.

4. Double-click the Compass for iCE software icon on the computer desktop to open the application.

5. Connect Maurice to Compass for iCE.

Running cIEF Applications

What You’ll Need

• Maurice cIEF Cartridges

• Maurice cIEF Method Development Kit (optional)

• Maurice System Suitability Kit (optional)

• Maurice cIEF Fluorescence Calibration Standard

• Maurice cIEF pI Markers (3.38, 4.05, 5.85, 6.14, 7.05, 8.4, 9.99, or 10.17)

• 0.5% Methyl Cellulose Solution

• 1% Methyl Cellulose Solution

• iCE Electrolyte Kit

• Deionized (DI) water

• Glass reagent vials, 2 mL

• 96-well plate or vials with integrated inserts for samples

•Clear screw caps for vials

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Running cIEF Applications page 83

• Blue pressure caps for vials

• P10, P20, P200, P1000 pipettes and tips

• Electrolyte pipette

•Vortexer

• Table-top centrifuge with plate adapter or vial adapter (12 mm, 2 mL vials)

Step 1: Prep Your Markers, Samples and Reagents

NOTES: You can prepare your samples to run either in 96-well plates or vials.

If you need to seal the 96-well plate during your run, we recommend the 4titude Pierceable Film (PN 4ti0566, 4titude). It can be used in both absorbance and native fluorescence modes. If you’re currently using X-Pierce adhesive film (PN XP-100, Excel Scientific), we recommend using it for absorbance mode only.

System Suitability Peptide Panel (Optional)

NOTES: Run the System Suitability Peptide Panel when you need to confirm performance on Maurice.

The System Suitability Peptide Panel is lyophilized. Always reseal the foil bag containing the unopened tubes with desiccant to prevent moisture absorption.

1. Using scissors, carefully cut the top the package off leaving the sealing strip intact.

2. Take out the strip of tubes and cut one clear tube of lyophilized System Suitability Peptide Panel from the strip. Return the remaining tubes to the original package, reseal tightly and store at 2-8°C.

3. Pierce the foil on the tube with a clean pipette tip.

4. Add 40 μL of DI water to the tube. Gently resuspend by pipetting up and down to mix.

5. Add 160 μL of the System Suitability Test Mix to the freshly reconstituted Peptide Panel. Gently mix by pipetting up and down. Transfer this solution to a 1.5 mL microcentrifuge tube.

6. Vortex the tube 3 times, 5 seconds each.

7. Centrifuge the tube at 10,000 xg for 3 minutes to sediment any particulates.

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Running cIEF Applications page 84

8. Carefully aspirate the top 160 μL of the solution and pipette it into a sample vial with integrated insert or well of a 96-well plate. You’ll want to insert the pipette tip all the way to the bottom of the insert or well when you dispense the solution to avoid introducing bubbles.

NOTE: Make sure to check for and remove any bubbles at the bottom of the sample vial or well.

9. If you're using vials, close the sample vial with a clear screw cap.

Clear screw cap

Sample vial with

integrated inser t

Samples

1. In a microcentrifuge tube, prepare your sample at a concentration of 1 mg/mL in a final volume of 40 μL in DI water.

2. In a separate tube, prepare IEF Separation Mix containing your chosen pI marker(s).

NOTE: Check out the Method Development Guide for suggested IEF Separation Mix recipes.

3. Add 160 μL of IEF Separation Mix to the 40 μL of your sample.

4. Vortex the tube 3 times, 5 seconds each.

5. Centrifuge the tube at 10,000 xg for 3 minutes to sediment any particulates

6. Carefully aspirate the top 160 μL of the sample and pipette it into your sample vial with integrated insert or well of a 96-well plate by inserting the pipette tip all the way to the bottom to avoid introducing bubbles.

Note: Make sure to check for and remove any bubbles at the bottom of the sample vial or well.

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Running cIEF Applications page 85

7. If you're using vials, close the sample vial with a clear screw cap.

pI Markers

1. Open the vial of lyophilized pI marker by lifting the center tab and gently pulling it back to break the metal seal. Slowly remove the rubber stopper.

2. Add 210 μL of DI water to the vial.

3. Put the rubber stopper back in the vial and vortex 3 times, 5 seconds each time to completely reconstitute the lyophilized cake. Repeat this step for all pI markers you're using.

4. Aliquot 20 μL of each reconstituted pI marker into separate tubes for storage.

NOTES: Keep your reconstituted pI markers on ice until you're ready to add them to your sample or IEF Separation Mix.

If you'll use the pI markers within a month, store the aliquots at 2-8 °C. Otherwise, store the aliquots at

-20 °C. They'll be stable up to 6 months.

5. Use 2 μL of each pI marker for every 200 μL of sample.

Reagents

NOTE: Don't reuse reagents, vials or the clear screw caps. Always keep the blue pressure caps paired with their respective reagents. If you want to reuse pressure caps, first wash them thoroughly with DI water, soak overnight in DI water, rinse caps thoroughly with DI water again and air dry them before reusing.

1. Pipette 2 mL of 0.5% Methyl Cellulose into a glass reagent vial, label and close with a

blue pressure cap.

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Running cIEF Applications page 86

Pressure cap

Glass reagent vial

2. Pipette 500 μL of Fluorescence Calibration Standard in a glass reagent vial, label and close with a

blue

pressure cap.

3. Pipette 2 mL of DI water into a glass reagent vial, label and close with a

4. Close an empty glass reagent vial with a

blue pressure cap.

5. Pipette 2 mL of DI water into a glass reagent vial, label and close with a clear screw cap.

Clear screw

cap

Glass reagent vial

Step 2: Prep the Cartridge

1. Take the cIEF Cartridge out of its packaging. Save the packaging, you’ll need it later to store the cartridge.

NOTE: When you remove the cartridge from its packaging, make sure the cartridge inlet doesn’t come in contact with any surface.

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Running cIEF Applications page 87

2. Put the cartridge on a flat surface with its electrolyte tanks facing up.

3. Remove the stoppers from both electrolyte tanks.

Anolyte

Tan k

4. Add 2 mL of Catholyte solution to the OH

5. Add 2 mL Anolyte solution to the H

electrolyte tank (red port).

NOTE: Make sure you don’t overfill the electrolyte tanks.

Optical

Window

Catholyte

Tan k

electrolyte tank (white port).

Cartridge

Inlet

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Running cIEF Applications page 88

6. Seal each tank with the rubber stoppers. Use the grey stopper for the OH- tank and the red one for the H+ tank. If excess liquid comes out of the tank, make sure to wipe it with a laboratory wipe.

Step 3: Install the Cartridge

1. Open Maurice’s door by touching the metal plate on top of the door.

NOTE: The indicator light on Maurice’s front panel will blink rapidly as the door disengages.

2. Swing the door open to access the cartridge slot and the reagents and samples platform. The lights on either side of the slot will be

User Guide for Maurice, Maurice C. and Maurice S.

orange.

Running cIEF Applications page 89

3. Double check to make sure you’ve got electrolytes loaded and the tanks are properly sealed with the stoppers.

4. Lift the cartridge and hold it vertically using the finger holds on either side, cartridge inlet down, with the cIEF label facing you.

5. Gently insert it into the slot. You’ll feel the alignment groove on the cartridge align inside the slot when you insert it.

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Running cIEF Applications page 90

6. Continue to slide the cartridge into the slot until the locking mechanism engages. The lights on either side of the slot will change to blue once the cartridge is installed correctly.

Step 4: Load Samples and Reagents

1. Place the reagent vials into their respective positions on the sample and reagents platform:

NOTES: The two reagent rows in Maurice’s reagent and sample platform are kept at room temperature.

Pressure caps are blue and have a raised, ringed surface. They should only be used in Reagent Row P. Use reagent vials with clear screw caps in Row N.

• P1 - 0.5% Methyl Cellulose with

blue pressure cap

• P2 - Fluorescence Calibration Standard with blue pressure cap

• P3 - Water vial with blue pressure cap

• P6 - Empty vial (air) with blue pressure cap

• N1 - Water vial with clear screw cap

User Guide for Maurice, Maurice C. and Maurice S.

ProteinSimple MAURICE Users Manual

Specifications and Main Features

Frequently Asked Questions

User Manual