ProteinSimple Jess User Manual
User Guide for Jess
page 1
Copyright © 2018 ProteinSimple. All rights reserved.
ProteinSimple
3001 Orchard Parkway
San Jose, CA 95134
Toll-free in the US and Canada: (888) 607-9692
Tel: (408) 510-5500
Fax: (408) 510-5599
email: support@proteinsimple.com
web: www.proteinsimple.com
User Guide for Jess
P/N PL2-0004
Revision B, November 2018
For research use only. Not for use in diagnostic procedures
Patents and Trademarks
ProteinSimple's Simple Western technology is covered by issued and pending patents in the U.S. and other
countries. For more information please see http://www.proteinsimple.com/Intellectual_Property.html.
ProteinSimple, the ProteinSimple logo and Jess are trademarks or registered trademarks of ProteinSimple.
Other marks appearing in these materials are marks of their respective owners.
Table of Contents
page i
Chapter 1:
Let’s Get Started. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Simple Western Assays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Imaging a Blot. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Chapter 2:
Getting Your Lab Ready . . . . . . . . . . . . . . . . . . . . 3
Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Physical Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Environmental Requirements. . . . . . . . . . . . . . . . . . . . . . . . . 6
Software and Computer Requirements . . . . . . . . . . . . . . . 6
Chapter 3:
Jess. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Chapter 4:
Operating Jess. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Running Immunoassays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Chapter 6:
General Information . . . . . . . . . . . . . . . . . . . . . . . 29
Safety Guidelines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Imaging a Blot. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Self Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Viewing and Changing System Properties . . . . . . . . . . . 22
Viewing Error and Test Logs. . . . . . . . . . . . . . . . . . . . . . . . . . 23
Chapter 5:
Maintenance and Troubleshooting . . . . . . 26
Instrument Software (Embedded) Updates . . . . . . . . . . 27
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
User Guide for Jess
page ii
User Guide for Jess
Chapter 1:
Let’s Get Started
Chapter Overview
•Welcome
page 1
• Simple Western Assays
• Imaging a Blot
User Guide for Jess
Welc ome page 2
Welcome
Congratulations on bringing Jess into your lab! We welcome you as a new user and are excited to be a part
of your work. This user guide will provide you with information on how Simple Western assays work as well
as other useful operating and installation information.
To help you get the most from Jess, we've added some attention phrases to assist you through the user
guide:
NOTE Points out useful information.
IMPORTANT Indicates information necessary for proper operation of Jess.
CAUTION Cautions you about potentially hazardous situations that could result in injury to you or
damage to Jess.
!WARNING! Warns you that serious physical injury can result if the listed precautions aren’t followed.
Simple Western Assays
A Simple Western size assay is an automated Western - no gels, no transfer devices, no blots, no film and no
manual analysis.
Simple Westerns take place in a capillary. Jess automates all steps of the process for you including sample
loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Up to 25
samples can be processed in a single run.
Jess performs Simple Western size-based assays: chemiluminescence or fluorescence immunoassays, and
intra-assay protein normalization. All you have to do is pipette your samples and immunoreagents into Jess's
pre-filled plate, load the capillary cartridge and press Start.
Imaging a Blot
Jess also images PVDF or nitrocellulose blotting membranes with proteins transferred from SDS-PAGE gels
using traditional Western blotting procedures. All you have to do is expose your membrane to an enhanced
chemiluminescent (ECL) substrate, load it into the blot cartridge and press Start.
User Guide for Jess
Chapter 2:
Getting Your Lab Ready
Chapter Overview
•Introduction
page 3
• Space Requirements
• Physical Specifications
• Electrical Requirements
• Environmental Requirements
• Software and Computer Requirements
• General Guidelines and Information
User Guide for Jess
Introduction page 4
Introduction
This chapter will help you prepare the lab for Jess. Please have the space, electrical and environmental
requirements ready prior to installation.
NOTE: Find a buddy to help you unpack Jess. Don’t try to lift the instrument alone, you could cause injury
to yourself or damage to Jess.
Space Requirements
You need a lab bench or table that can support 60 lbs (27 kg) and has enough space for both Jess and her
computer. There should be sufficient clearance for both heat ventilation and to provide access if the instru
ment needs service.
IMPORTANT
Jess needs a stable surface and must remain level to work properly. The lab bench or table can’t
shift or wobble under heavy weight.
-
Dimension Meters Feet
Width 2.2 7.0
Depth 0.9 3.0
Height 1.0 3.3
Table 2-1: Recommended space requirements for Jess.
User Guide for Jess
Physical Specifications page 5
Physical Specifications
Description Specification
Jess’s Dimensions (Door Closed) 0.36 m H x 0.36 m W x 0.57 m D
(1.08' H x 1.08' W x 1.71' D)
Jess’s Dimensions (Door Open) 0.36 m H x 0.53 m W x 0.57 m D
(1.08' H x 1.58' W x 1.71' D)
Jess’s Weight 23 kg (50 lbs)
Computer Workstation Dimensions 0.41 m H x 0.66 m W x 0.76 m D
(1.35' H x 2.17' W x 2.49' D)
For indoor use only. Use up to altitudes of 1524 meters (5000 feet).
Table 2-2: Physical Specifications
Electrical Requirements
Jess requires a dedicated, grounded circuit capable of delivering the appropriate current and voltage for
your country. The power requirements for select regions are listed below:
Region Volts (AC) Frequency (Hz) Amps
US and Canada 120 60 4.2
Europe 240 50 2.1
Japan 100 50/60 5.0
Table 2-3: Power requirements.
In addition to the requirements listed above, we recommend the grounded circuits terminate at the receptacles, and receptacles must be located within 10 ft (3 m) of the instrument.
User Guide for Jess
Environmental Requirements page 6
Environmental Requirements
Jess likes a consistent temperature in the lab. She works best when conditions stay within these ranges:
Requirement Specification
Operating temperature range 18–24 °C (64–75 °F)
Operating humidity range 20–60% relative, non-condensing
Table 2-4: Environmental requirements.
Software and Computer Requirements
Jess brings her own computer to the lab with Compass for Simple Western software pre-installed. Compass
software is used to run assays and analyze resulting data. Just in case you need it, a CD containing Compass
for Simple Western also comes in the box. If you don't want to analyze your data at Jess's workstation in the
lab, Compass software can also be installed on a separate workstation, such as your desktop computer. Your
computer must meet the minimum requirements listed in the table below to run the software and process
data.
Component Minimum Recommended
Operating system Windows 7
Processor Core 2 Duo
Memory 2 GB
Free disk space 10 GB
Table 2-5: Computer requirements.
User Guide for Jess
General Guidelines and Information page 7
General Guidelines and Information
Intended Use
NOTE: Jess is for research use only. Not for use in diagnostic procedures.
Lifting and Moving the System: Lift Jess Correctly
IMPORTANT
Take all the standard precautions when lifting or moving Jess. Since Jess weighs 23 kg (50 lbs), you
should not lift the instrument by yourself. Two people should lift the instrument onto the lab bench.
Do not lift the instrument by holding the door or door hinge.
User Guide for Jess
Chapter 3:
Jess
Chapter Overview
• Instrument Overview
page 8
User Guide for Jess
Instrument Overview page 9
Tou ch Pad to
System Door
Status Light
Open Door
Instrument Overview
Jess’s individual hardware components are described in this section.
External Components
System Door
Jess's door gives you access to the inside of the instrument to load the blot cartridge or capillary cartridge
and pre-filled plate. To open the door, make sure that you see a steady blue LED on the front, then touch the
small silver touch pad on the top of Jess’s door. To close, push the door until you feel Jess pull it closed.
NOTE: Jess’s door must be closed before starting a run.
User Guide for Jess
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