User Guide for Jess
page 1
Copyright © 2018 ProteinSimple. All rights reserved.
ProteinSimple
3001 Orchard Parkway
San Jose, CA 95134
Toll-free in the US and Canada: (888) 607-9692
Tel: (408) 510-5500
Fax: (408) 510-5599
email: support@proteinsimple.com
web: www.proteinsimple.com
User Guide for Jess
P/N PL2-0004
Revision B, November 2018
For research use only. Not for use in diagnostic procedures
Patents and Trademarks
ProteinSimple's Simple Western technology is covered by issued and pending patents in the U.S. and other
countries. For more information please see http://www.proteinsimple.com/Intellectual_Property.html.
ProteinSimple, the ProteinSimple logo and Jess are trademarks or registered trademarks of ProteinSimple.
Other marks appearing in these materials are marks of their respective owners.
Table of Contents
page i
Chapter 1:
Let’s Get Started. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Simple Western Assays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Imaging a Blot. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Chapter 2:
Getting Your Lab Ready . . . . . . . . . . . . . . . . . . . . 3
Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Physical Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Environmental Requirements. . . . . . . . . . . . . . . . . . . . . . . . . 6
Software and Computer Requirements . . . . . . . . . . . . . . . 6
Chapter 3:
Jess. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Chapter 4:
Operating Jess. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Running Immunoassays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Chapter 6:
General Information . . . . . . . . . . . . . . . . . . . . . . . 29
Safety Guidelines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Imaging a Blot. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Self Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Viewing and Changing System Properties . . . . . . . . . . . 22
Viewing Error and Test Logs. . . . . . . . . . . . . . . . . . . . . . . . . . 23
Chapter 5:
Maintenance and Troubleshooting . . . . . . 26
Instrument Software (Embedded) Updates . . . . . . . . . . 27
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
User Guide for Jess
page ii
User Guide for Jess
Chapter 1:
Let’s Get Started
Chapter Overview
•Welcome
page 1
• Simple Western Assays
• Imaging a Blot
User Guide for Jess
Welc ome page 2
Welcome
Congratulations on bringing Jess into your lab! We welcome you as a new user and are excited to be a part
of your work. This user guide will provide you with information on how Simple Western assays work as well
as other useful operating and installation information.
To help you get the most from Jess, we've added some attention phrases to assist you through the user
guide:
NOTE Points out useful information.
IMPORTANT Indicates information necessary for proper operation of Jess.
CAUTION Cautions you about potentially hazardous situations that could result in injury to you or
damage to Jess.
!WARNING! Warns you that serious physical injury can result if the listed precautions aren’t followed.
Simple Western Assays
A Simple Western size assay is an automated Western - no gels, no transfer devices, no blots, no film and no
manual analysis.
Simple Westerns take place in a capillary. Jess automates all steps of the process for you including sample
loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Up to 25
samples can be processed in a single run.
Jess performs Simple Western size-based assays: chemiluminescence or fluorescence immunoassays, and
intra-assay protein normalization. All you have to do is pipette your samples and immunoreagents into Jess's
pre-filled plate, load the capillary cartridge and press Start.
Imaging a Blot
Jess also images PVDF or nitrocellulose blotting membranes with proteins transferred from SDS-PAGE gels
using traditional Western blotting procedures. All you have to do is expose your membrane to an enhanced
chemiluminescent (ECL) substrate, load it into the blot cartridge and press Start.
User Guide for Jess
Chapter 2:
Getting Your Lab Ready
Chapter Overview
•Introduction
page 3
• Space Requirements
• Physical Specifications
• Electrical Requirements
• Environmental Requirements
• Software and Computer Requirements
• General Guidelines and Information
User Guide for Jess
Introduction page 4
Introduction
This chapter will help you prepare the lab for Jess. Please have the space, electrical and environmental
requirements ready prior to installation.
NOTE: Find a buddy to help you unpack Jess. Don’t try to lift the instrument alone, you could cause injury
to yourself or damage to Jess.
Space Requirements
You need a lab bench or table that can support 60 lbs (27 kg) and has enough space for both Jess and her
computer. There should be sufficient clearance for both heat ventilation and to provide access if the instru
ment needs service.
IMPORTANT
Jess needs a stable surface and must remain level to work properly. The lab bench or table can’t
shift or wobble under heavy weight.
-
Dimension Meters Feet
Width 2.2 7.0
Depth 0.9 3.0
Height 1.0 3.3
Table 2-1: Recommended space requirements for Jess.
User Guide for Jess
Physical Specifications page 5
Physical Specifications
Description Specification
Jess’s Dimensions (Door Closed) 0.36 m H x 0.36 m W x 0.57 m D
(1.08' H x 1.08' W x 1.71' D)
Jess’s Dimensions (Door Open) 0.36 m H x 0.53 m W x 0.57 m D
(1.08' H x 1.58' W x 1.71' D)
Jess’s Weight 23 kg (50 lbs)
Computer Workstation Dimensions 0.41 m H x 0.66 m W x 0.76 m D
(1.35' H x 2.17' W x 2.49' D)
For indoor use only. Use up to altitudes of 1524 meters (5000 feet).
Table 2-2: Physical Specifications
Electrical Requirements
Jess requires a dedicated, grounded circuit capable of delivering the appropriate current and voltage for
your country. The power requirements for select regions are listed below:
Region Volts (AC) Frequency (Hz) Amps
US and Canada 120 60 4.2
Europe 240 50 2.1
Japan 100 50/60 5.0
Table 2-3: Power requirements.
In addition to the requirements listed above, we recommend the grounded circuits terminate at the receptacles, and receptacles must be located within 10 ft (3 m) of the instrument.
User Guide for Jess
Environmental Requirements page 6
Environmental Requirements
Jess likes a consistent temperature in the lab. She works best when conditions stay within these ranges:
Requirement Specification
Operating temperature range 18–24 °C (64–75 °F)
Operating humidity range 20–60% relative, non-condensing
Table 2-4: Environmental requirements.
Software and Computer Requirements
Jess brings her own computer to the lab with Compass for Simple Western software pre-installed. Compass
software is used to run assays and analyze resulting data. Just in case you need it, a CD containing Compass
for Simple Western also comes in the box. If you don't want to analyze your data at Jess's workstation in the
lab, Compass software can also be installed on a separate workstation, such as your desktop computer. Your
computer must meet the minimum requirements listed in the table below to run the software and process
data.
Component Minimum Recommended
Operating system Windows 7
Processor Core 2 Duo
Memory 2 GB
Free disk space 10 GB
Table 2-5: Computer requirements.
User Guide for Jess
General Guidelines and Information page 7
General Guidelines and Information
Intended Use
NOTE: Jess is for research use only. Not for use in diagnostic procedures.
Lifting and Moving the System: Lift Jess Correctly
IMPORTANT
Take all the standard precautions when lifting or moving Jess. Since Jess weighs 23 kg (50 lbs), you
should not lift the instrument by yourself. Two people should lift the instrument onto the lab bench.
Do not lift the instrument by holding the door or door hinge.
User Guide for Jess
Chapter 3:
Jess
Chapter Overview
• Instrument Overview
page 8
User Guide for Jess
Instrument Overview page 9
Tou ch Pad to
System Door
Status Light
Open Door
Instrument Overview
Jess’s individual hardware components are described in this section.
External Components
System Door
Jess's door gives you access to the inside of the instrument to load the blot cartridge or capillary cartridge
and pre-filled plate. To open the door, make sure that you see a steady blue LED on the front, then touch the
small silver touch pad on the top of Jess’s door. To close, push the door until you feel Jess pull it closed.
NOTE: Jess’s door must be closed before starting a run.
User Guide for Jess
Instrument Overview page 10
Status Lights
The LED on Jess’s front panel provides you with status updates:
• Start-up (magenta): You've turned on the power and the instrument is warming up.
• Ready (steady blue): Jess is powered on and ready for use.
• Opening Door (long blue flash followed by blue pulses): Jess’s door is opening.
• Running (pulsing blue): Jess is running an assay.
• Trying to Open Door While Running (red flash): Jess can’t open the door when she’s running.
• Error (steady red): Jess has detected an error. To get more information, check the Status window of
the Run Summary Screen in Compass for Simple Western.
User Guide for Jess
Instrument Overview page 11
Cartridge Holder
(Capillary)
Cartridge Holder
(Blot)
Internal Components
Cartridge Holder
The cartridge holder will hold:
• The 25-Capillary Cartridge (PS-CC01)
• The 13-Capillary Cartridge (PS-CC02)
• The Blot Cartridge (PN 004-651) containing a loaded membrane
User Guide for Jess
Instrument Overview page 12
Plate
Holder
A1 Position
Sample Plate
Plate Holder
The plate holder holds the pre-filled plate which contains samples and assay reagents.
Sample plates should be inserted into the plate holder so that the A1 well position is aligned with the upper
left corner of the tray.
User Guide for Jess
Instrument Overview page 13
NOTES:
Remove the lid and seal from the plate immediately before inserting into the instrument.
When inserting the sample plate, make sure the plate is firmly seated and level in the tray. Plates that
aren’t level can interfere with the movement of the plate holder.
Rear Panel
Located on Jess’s rear panel is the power entry, power switch and the network connection port.
• System Power - The main system power components consist of the power switch and power input.
!WARNING!
Only use the power supply cord provided with Jess. If the cord is damaged, please contact
ProteinSimple Technical Support toll-free in the US and Canada at 1-888-607-9692 (option 3).
!WARNING!
You can't replace any parts except the power entry fuse.
!WARNING! SHOCK HAZARD
Disconnect the power cord from the power input to disconnect power to the instrument.
• Connect to PC - A 10/100/BASE-T Ethernet (RJ-45 connector) is used to connect Jess to a computer.
User Guide for Jess
Instrument Overview page 14
50/60HZ 510W
415-0004 REV A
250VAC 6.3A
San Jose, CA USA
Model: Jess
Made in USA
Pat: proteinsimple.com/Intellectual_Property
NOTE: Serial numbers are used to identify individual instruments on the network.
System Label
A system label is also located on Jess’s rear panel. It includes the ProteinSimple location, system model,
power requirements, serial number and certification markings.
User Guide for Jess
Chapter 4:
Operating Jess
Chapter Overview
• Before You Throw the Switch
page 15
•Power Up
• Running Immunoassays
• Imaging a Blot
•Self Test
• Viewing and Changing System Properties
•Viewing Error and Test Logs
•Status Modes
•Shutdown
User Guide for Jess
Before You Throw the Switch page 16
Before You Throw the Switch
Ensure that everyone using Jess has:
• Received instruction in general safety practices for laboratories.
• Received instruction in specific safety practices for Jess.
• Received instruction on handling of biohazards (if biohazardous materials are to be used on Jess).
• Read and understood all related SDS documentation.
Power Up
1. Turn on the computer connected to Jess.
2. Turn on the main power switch located on the rear panel.
3. Wait for Jess to initialize.
4. Double-click the Compass for Simple Western software icon to open the application.
5. Connect to the Jess instrument.
6. Run the Self Test.
Running Immunoassays
Step 1 - Get Ready
1. Create or open your assay file in Compass for Simple Western.
2. Prepare the assay plate following the procedure in the product insert.
NOTE: The first capillary in the cartridge has been optimized for running the ready-to-use biotinylated
ladder. Using it for a sample may increase your assay variability.
3. Open a capillary cartridge package and insert the cartridge into the cartridge holder. The light in the
cartridge holder will change from orange to blue when inserted correctly.
4. Remove the plate lid and seal and place the assay plate into the plate holder.
5. Click Start.
User Guide for Jess
Running Immunoassays page 17
IMPORTANT
Capillaries are light sensitive. Keep the cartridge in the sealed package until you are ready to insert
the capillary cartridge into the instrument.
To prevent well evaporation and to get the best results, keep a lid on the assay plate until your are
ready to use it. Once you've pipetted your sample and reagents into the plate, it can't be stored for
later use.
Step 2 - Start the Run
You can start an immunoassay two ways depending on whether you want to run an assay using existing
parameters or set up a new assay.
1. To start a new run with an existing assay:
a. Select File in the main menu and click Open Assay.
b. A list of the last five assays opened will display. Select one of these assays or click Browse to open
the Assay folder and select a different one.
2. To start a run with a new assay:
a. Select File in the main menu, click New Assay. From the pop-up window, select the Assay Type
(Chemiluminescence, Fluorescence and/or Protein Normalization), Size Range (2-40 kDa,
12-230 kDa or 66-440 kDa) and Cartridge (either the 25 or 13 Capillary Cartridge).
User Guide for Jess
Running Immunoassays page 18
b. The Start button will display, which means an assay is already loaded.
c. Go to the Assay screen and make sure it’s the assay you want to use. If not, select File in the main
menu, click Open Assay, and select the correct one.
3. Click Start. The Start Run window for immunoassays displays with the default run file name and loca-
tion. Click Browse if you want to save it in a different location.
User Guide for Jess
Running Immunoassays page 19
Click Start to begin the run. Jess’s status will change to Running, the Start button will change to Stop
and the Run Progress Bar will appear:
Once she’s started, Jess will run all your samples to completion in about 3 hours depending on the
assay. To check on the time remaining, click the Run Summary tab in the upper right corner of the
screen.
NOTE: Jess will continue to run even if Compass for Simple Western is closed. Always check the status light
to confirm whether Jess is performing a run.
Step 3 - Post-Run Procedures
When the run is done, you’ll need to:
1. Remove the capillary cartridge.
2. Remove the sample plate.
Capillary and sample plate disposal depends on the samples that were assayed. If you aren’t sure what your
sample origins are, we recommend used cartridges and plates be disposed of as biohazard waste.
!WARNING! SHARPS HAZARD
Capillaries may present a potential sharps hazard. Dispose of used capillaries according to your institution's sharps disposal policy.
User Guide for Jess
Imaging a Blot page 20
!WARNING! BIOHAZARD
Capillary cartridges and sample plates should be handled by procedures recommended
in the CDC/NIH manual: Biosafety in Microbiological and Biomedical Laboratories (BMBL).
The manual is available from the U.S. Government Printing Office or online at
http://www.cdc.gov/biosafety/publications/bmbl5/.
Depending on the samples used, the capillary cartridge and plate may constitute a biohazard. Dispose of the cartridge and plate in accordance with good laboratory practices
and local, state/provincial and national environmental and health regulations. Read and
understand the Safety Data Sheets (SDSs) provided by the manufacturers of the chemicals before you store, handle or dispose of chemical waste.
Stopping a Run
1. To stop an immunoassay, click Stop. When the run stops, the Start button with reappear and she’ll start
a process that plugs the capillaries in the cartridge. The stopped status, date and time will display in the
Run Summary screen.
2. Once that’s done, you can remove the capillary cartridge and plate and discard them.
Imaging a Blot
Step 1 - Get Ready
1. Open Compass for Simple Western software on your desktop.
2. Make sure that the software is connected to the instrument.
3. Prepare the membrane and assemble the blot cartridge following the product insert.
4. Insert the blot cartridge containing the membrane into the cartridge holder. The light in the cartridge
holder will change from orange to blue when inserted correctly.
5. Close the instrument door by gently pushing it until you feel Jess pull it closed. Jess automatically
detects the blot cartridge and displays a preview image in the Start Run window for imaging.
NOTE: Jess's door must be closed before starting image acquisition.
User Guide for Jess
Imaging a Blot page 21
Step 2 - Start the Run
1. Edit the settings in the Exposure table:
• To add an exposure: Click Add then select the exposure time to change it.
• To remove an exposure: Select the exposure and click Remove.
• To edit exposure time: Click on an exposure and enter a new time.
2. Check the Markers box if the blot contains a molecular weight ladder.
3. Change the file name and location if desired, and click Start to begin imaging.
Step 3 - Post-Run Procedures
When imaging is done, you'll need to:
1. Remove the blot cartridge.
2. Remove your membrane and clean the blot cartridge by rinsing it thoroughly with water. Dry with a lint-
free wipe.
3. Wipe down the area under the blot holder inside the instrument using a dry lint-free wipe.
User Guide for Jess
Self Test page 22
4. Keep the blot cartridge in a clean, dry area and protected from dust.
Stopping a Run
To stop an imaging run, click Stop and confirm your choice. When the run stops, the Start button will reappear so you can start another run when you’re ready.
Self Test
Jess can perform a set of self tests to make sure she’s operating properly. To start the testing, select
Instrument in the main menu and click Self Test. Testing will take about 30 minutes.
NOTE: We recommend running the self test before you begin preparing your samples, reagents and the
assay plate.
To view the log when the test is done, select Instrument, click Properties and click Logs. Select the test log
you would like to view and click View.
To view the self test results click Report.
Viewing and Changing System Properties
From the Compass for Simple Western main menu, select Instrument and click Properties to see Jess’s system properties:
•Name
User Guide for Jess
Viewing Error and Test Logs page 23
Insert image here.
•Location
• Type
• Serial number
• Instrument software version (firmware)
• Network name and address
• Date and time of the instrument clock
• To change system name or location: Click in the name or location boxes and enter the new infor-
mation.
• To sync the instrument clock with the computer: Click Set to PC time.
Viewing Error and Test Logs
From the Compass for Simple Western main menu, select Instrument and click Properties. To view a log,
click Logs.
User Guide for Jess
Viewing Error and Test Logs page 24
The table that displays shows the list of Self Test logs. You can also select Command Log or Instrument Log
to view and save logs related to the instrument and software.
User Guide for Jess
Status Modes page 25
Status Modes
The status bar displays status, buttons and progress bars.
• Ready/Start button - Jess is ready and an assay is loaded. Click Start to begin a run.
• Running/Stop button - Jess is running an assay. The run name, time it started and when it will be
done are shown in the run progress bar. Click Stop to stop the run.
• Error/Reset button - Jess has detected an error. You can go to the Status window in the
Run Summary screen to get more details. Once you’ve been able to correct the error, click Reset.
Shutdown
1. Make sure to remove any plates, cartridges and blots before shutting down the system.
2. Close Compass for Simple Western and shut down Jess’s computer.
3. Jess should remain on unless you think she won’t be used for more than a week. In that case, you can
just turn the power off.
User Guide for Jess
Chapter 5:
Maintenance and
Troubleshooting
page 26
Chapter Overview
•Software Updates
• Instrument Software (Embedded) Updates
•Maintenance
• Troubleshooting
User Guide for Jess
Software Updates page 27
Software Updates
To check for software updates, go to Compass for Simple Western, select Help in the main menu and click
Check for Updates.
Instrument Software (Embedded) Updates
To view the version installed, open Compass for Simple Western, select Instrument in the main menu, then
click Properties. The current version of system software will be displayed.
To check for embedded updates, go to Compass for Simple Western, select Instrument in the main menu,
then Update and select Network. If you are not on the network, contact ProteinSimple Technical Support
for assistance on how to obtain the latest update.
Maintenance
Daily
The following cleaning procedures should be performed on a daily basis.
Clean the Cartridge
• For Capillary Cartridge: Dispose of the used capillary cartridge and sample plate after each run.
• For Blot Cartridge: Use a lint-free cloth and wipe down the glossy surface to prevent image artifacts.
Also be sure to wipe down the black door liner prior to use to prevent image artifacts and high background.
Clean the Door Liner
The door liner must be cleaned before every run with Jess to avoid fluorescence of contaminants during
image capture.
1. Open Jess' door to reveal the door liner (glossy black finish area behind the door).
User Guide for Jess
Troubleshooting page 28
Door liner
2. Spray 70% isopropyl alcohol onto one of the provided Polyester Cleanroom lint-free wipes (PN 104-
0031) or equivalent.
3. Carefully wipe down the door liner with the moist wipe. Avoid touching the door liner with your hands.
4. Wait 30 seconds for the solvent to evaporate before using Jess.
Yea rl y
We recommend Jess has annual preventive maintenance performed by an authorized ProteinSimple
representative. Please contact your Simple Western Sales Specialist or Technical Support to schedule a visit
toll-free in the US and Canada at 1-888-607-9692 (option 3).
Troubleshooting
For Jess and Simple Western assay troubleshooting information, please contact ProteinSimple Technical
Support toll-free in the US and Canada at (888) 607-9692 (option 3), support@proteinsimple.com or visit
http://www.proteinsimple.com/resources.html?product=simple-western. You can also contact your local
Field Application Scientist for help.
User Guide for Jess
Chapter 6:
General Information
Chapter Overview
•Compliance
page 29
• Safety Guidelines
• Customer Service and Technical Support
•Legal Notices
User Guide for Jess
Compliance page 30
Compliance
Jess complies with:
• UL 61010-1:2001: Safety requirements for electrical equipment for measurement, control and
laboratory use - Part 1: General requirements (US)
• EN 61010-1:2001: Safety requirements for electrical equipment for measurement, control and
laboratory use - Part 1: General requirements (EU)
• CAN/CSA 22.2 No. 61010-1-04: Safety requirements for electrical equipment for measurement,
control and laboratory use - Part 1: General requirements (CA)
• EN 61326-1:2006: Electrical equipment for measurement, control and laboratory use. EMC
Requirements. General requirements (EU)
Safety Guidelines
!WARNING!
If Jess is not used as specified by ProteinSimple, overall safety will be impaired.
!WARNING!
If Jess is damaged and doesn’t function properly, stop the instrument safely and contact ProteinSimple
Technical Support right away toll-free in the US and Canada at 1-888-607-9692 (option 3).
!WARNING!
You cannot replace or service any parts.
CAUTION
Avoid using Jess in ways not specified by ProteinSimple. Although the instrument has been
designed to protect you, this protection may not be effective if the instrument isn’t used properly.
User Guide for Jess
Safety Guidelines page 31
Chemical Hazards
!WARNING! CHEMICAL HAZARD
Some chemicals used can be potentially hazardous, and can cause injury or illness.
• Read and understand the Safety Data Sheets (SDSs) provided by the chemical manufacturer before
you store, handle or work with any chemicals or hazardous materials.
• Minimize contact with and inhalation of chemicals. Wear appropriate personal protective equipment
when handling chemicals (e.g., safety glasses, gloves, clothing). For additional safety guidelines, consult the SDS.
• Do not leave chemical containers open.
• Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer's cleanup
procedures as recommended by the SDS.
• Comply with all local, state/provincial and national laws and regulations related to chemical storage,
handling and disposal.
Chemical Waste Hazards
!WARNING! BIOHAZARD
Capillary cartridges and sample plates should be handled by procedures recommended
in the CDC/NIH manual: Biosafety in Microbiological and Biomedical Laboratories (BMBL).
The manual is available from the U.S. Government Printing Office or online at
http://www.cdc.gov/biosafety/publications/bmbl5/.
Depending on the samples used, the capillary cartridge and plate may constitute a biohazard. Dispose of the cartridge and plate in accordance with good laboratory practices
and local, state/provincial and national environmental and health regulations.
• Read and understand the Safety Data Sheets (SDSs) provided by the manufacturers of the chemicals
in a waste container before you store, handle or dispose of chemical waste.
• Minimize contact with chemical waste. Wear appropriate personal protective equipment when handling chemicals (e.g., safety glasses, gloves, clothing).
User Guide for Jess
Safety Guidelines page 32
Safety Data Sheets
Some chemicals used with Jess may be listed as hazardous. Warnings are displayed on the labels of all
chemicals when hazards exist.
Safety Data Sheets (SDSs) provide users with safety information needed to store, handle, transport and dispose of the chemicals safely. We recommend updating laboratory SDS records periodically.
SDSs for ProteinSimple reagents are available online at http://www.proteinsimple.com/technical_li-
brary.html or by calling toll-free in the US and Canada 1-888-607-9692. Otherwise, call the chemical manu-
facturer directly or visit their web site.
Instrument Safety Labels
The following safety labels are located on Jess. Each label will display a safety alert symbol indicating a
potential safety hazard.
Symbol Description
Risk of Electric Shock.
Refer to Jess User Guide before proceeding.
Danger of hazardous waste. Use caution in these areas. This warning only applies if using
hazardous material. Jess reagents are not considered hazardous waste. If you are using
hazardous materials, please contact your field service representative to place labels in
the appropriate locations.
User Guide for Jess
Customer Service and Technical Support page 33
Customer Service and Technical Support
Te le ph on e
(408) 510-5500
(888) 607-9692 (toll-free in the US and Canada)
Fax
(408) 510-5599
E-mail
support@proteinsimple.com
Web
www.proteinsimple.com
Address
ProteinSimple
3001 Orchard Parkway
San Jose, CA 95134
USA
User Guide for Jess
Legal Notices page 34
Legal Notices
NOTE: Read the Legal Notices carefully before using Jess.
Jess Disclaimer of Warranty
EXCEPT AS EXPRESSLY PROVIDED IN ANY ProteinSimple SOFTWARE LICENSE AGREEMENT OR QUOTATION, THE PRODUCTS SOLD AND SERVICES PROVIDED BY ProteinSimple ARE PROVIDED ON AN “AS IS” AND “AS AVAILABLE” BASIS WITHOUT WARRANTY OF ANY KIND. ProteinSimple
AND ITS SUPPLIERS DO NOT WARRANT THE SECURITY, PRIVACY OR ACCURACY OF ANY DATA PROVIDED VIA THE PRODUCTS OR SERVICES, AND
YOU AGREE THAT THE USE OF ANY SUCH DATA BY YOU IS AT YOUR SOLE RISK. TO THE MAXIMUM EXTENT ALLOWED UNDER APPLICABLE LAW,
ProteinSimple AND ITS SUPPLIERS DISCLAIM ANY AND ALL WARRANTIES, WHETHER EXPRESS, IMPLIED OR STATUTORY, INCLUDING, WITHOUT
LIMITATION, ANY WARRANTY OF MERCHANTABILITY, TITLE, NON-INFRINGEMENT OR FITNESS FOR A PARTICULAR PURPOSE.
Compass for Simple Western Software and Authorization Server License Agreement
IMPORTANT - PLEASE READ CAREFULLY THE TERMS OF THIS COMPASS SOFTWARE AND AUTHORIZATION SERVER LICENSE AGREEMENT
(“AGREEMENT”). BY CLICKING ON THE “I AGREE” BUTTON, (1) YOU ACKNOWLEDGE THAT YOU HAVE READ, UNDERSTAND AND AGREE TO BE
BOUND BY THIS AGREEMENT AND (2) YOU REPRESENT THAT YOU HAVE THE AUTHORITY TO ENTER INTO THIS AGREEMENT, PERSONALLY OR IF
YOU HAVE NAMED A COMPANY AS CUSTOMER, ON BEHALF OF THAT COMPANY (YOU OR ANY SUCH COMPANY, THE “CUSTOMER”), AND TO
BIND THE CUSTOMER TO THE TERMS OF THIS AGREEMENT. IF YOU DO NOT AGREE TO ALL TERMS AND CONDITIONS OF THIS AGREEMENT, OR
IF YOU DO NOT HAVE S UCH AUTHOR ITY, YOU S HOULD CLICK ON TH E “CANCEL” BUTTON TO DISCONTINUE THE DOWNLOAD OF THE LICENSED
SOFTWARE.
1. Definitions
1.1 “Authorized Use Parameters” means the following usage restrictions, which restrict the operation of the Licensed Software to a
particular set of conditions: Customer shall (a) limit simultaneous use of the Licensed Software to a maximum of ten (10) Authorized
Users; and (b) use the Licensed Software only in connection with the accompanying System purchased by Customer pursuant to the
System Quotation and located at the Site.
1.2 “Authorized User” means one (1) User who initiates the execution of the Licensed Software and/or interacts with or directs the
Licensed Software in the performance of its functions. Multiple Authorized Users may work simultaneously with one installation of the
Licensed Software, as on a server, or they may each have their own installation on single-user machines, or a mix of these, provided
that in all cases the total number of simultaneous Users does not exceed the applicable Authorized Use Parameters.
1.3 “Company” means ProteinSimple.
1.4 “Documentation” means Company's then-current manuals, guides and online help pages, if any, applicable to the Licensed Software
and made generally available by Company to its customers.
1.5 “Enterprise” means those organizations that have Internet addresses located at top level and second-level domain names set forth in
the System Quotation.
1.6 “Error” means a reproducible error in the Licensed Software that prevents such Licensed Software from operating substantially in
accordance with its Documentation.
1.7 “Executable Code” means the fully compiled binary version of Licensed Software that can be executed by a computer and used by
an end user without further compilation.
1.8 “Intellectual Property Rights” means all copyrights, trade secrets, patents, patent applications, moral rights, contract rights and
other proprietary rights, but specifically excluding any trademarks or service marks.
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1.9 “Licensed Software” means the Compass for Simple Western software program in Executable Code form, and any Updates that
Company makes available to Customer in accordance with this Agreement.
1.10 “Site” means the facility or campus set forth in the System Quotation.
1.11 “System” means the proprietary NP1000, NP100, Simon, Sally, Peggy, Wes, Jess, Sally Sue and Peggy Sue protein analysis system or any
future model or successor thereto that is provided to Customer by Company pursuant to a separate agreement between the parties
(the “System Quotation”).
1.12 “Update” means those releases of the Licensed Software that Company provides to customers to correct Errors, fix bugs, or create
minor improvements, incremental features or enhancements of existing features which Company designates by a change in the
number to the right of the first or second decimal point. Updates do not include those releases of the Licensed Software that provide
substantial new features or additional functionality which Company designates by a change in the number to the left of the first
decimal point.
1.13 “User” means any individual that has an e-mail address within the Enterprise.
2. License and Restrictions
2.1 License Grant. Subject to the terms and conditions of this Agreement and the payment of the required fees set forth in the System
Quotation, Company grants to Customer a nontransferable, nonexclusive, royalty-free, revocable, worldwide license (without the right
to sublicense) to (a) install the Licensed Software on any computer located at any Site; (b) use, execute and display the Licensed
Software, in Executable Code form only; and (c) copy the Licensed Software and Documentation, solely as necessary to support
Authorized Users; in each of the foregoing, solely in accordance with the Documentation and the Authorized Use Parameters.
Customer agrees that it will comply with the Authorized Use Parameters.
2.2 License Restrictions. Customer acknowledges that the Licensed Software and its structure and organization constitute valuable
trade secrets of Company. Accordingly, the license granted in this Agreement is subject to the following restrictions: Customer and its
Authorized Users (a) may not reverse engineer, disassemble, decompile or otherwise attempt to derive the source code of Licensed
Software; (b) may not modify, adapt, alter, translate or create derivative works from the Licensed Software; (c) may not merge the
Licensed Software with other software; (d) may not use the Licensed Software in any service bureau or time-sharing arrangement,
license, sell, rent, lease, transfer, assign, distribute, host, outsource, disclose or otherwise commercially exploit or make the Licensed
Software or Documentation available to any third party; (e) shall only make that number of exact copies of the Licensed Software and
Documentation as delivered by Company that are necessary to support Customer's use of the Licensed Software in accordance with
this Agreement; (f) shall include any titles, trademarks and copyright and restricted rights notices that are included on or in the
Licensed Software as delivered by Company on and in any copies of the Licensed Software that it makes; and (g) shall ensure that
Customer's use of the Licensed Software does not exceed the scope of the license that Customer has purchased pursuant to this
Agreement.
2.3 Open Source Software. Certain items of independent, third-party code may be included in the Licensed Software that are subject to
open source licenses (“Open Source Software”). Such Open Source Software is licensed under the terms of the license that
accompanies such Open Source Software. Nothing in this Agreement limits Customer's rights under, or grants Customer rights that
supersede, the terms and conditions of any applicable end user license for such Open Source Software. In particular, nothing in this
Agreement restricts Customer's right to copy, modify and distribute such Open Source Software that is subject to the terms of such
open source licenses.
2.4 Ownership. Company reserves all rights not expressly granted to Customer in this Agreement. Without limiting the generality of the
foregoing, Customer acknowledges and agrees that, except as expressly set forth in this Agreement, Company and its suppliers retain
all Intellectual Property Rights, title and interest in and to the Licensed Software and Documentation.
3. Support and Maintenance Services
3.1 Services. Subject to Customer's payment of the Services fees, as set forth in the System Quotation, and to the terms and conditions
herein, Company will use commercially reasonable efforts to provide to Customer the following support and maintenance services
(the “Services”) for the Licensed Software: (a) Company will answer technical questions concerning functions and features of the
Licensed Software; (b) Company will provide Error verification, analysis and corrective efforts for the Licensed Software; and (c)
Company will provide, without charge, Updates of the software released during the term of this Agreement. Customer will be
responsible for providing, in a manner consistent with good industry practice, all Services to Users. Customer acknowledges that
Company may not be able to correct all reported Errors. Any Update of the Licensed Software will be deemed part of the Licensed
Software and Customer will use such Updates in accordance with the requirements and obligations in this Agreement.
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3.2 Service Conditions. Company's obligation to provide the Services is conditioned on Customer: (a) notifying Company of any Error
within a reasonable period of time; (b) providing Company all information relating to the Error; (c) providing access to the Licensed
Software and Customer's facility where the Licensed Software is located and informing Company of any potential hazards which may
be encountered while servicing the Licensed Software. Customer may contact Company via telephone toll-free in the US and Canada
1-888-607-9692 or e-mail at support@proteinsimple.com during the hours of 8:00 AM (Pacific Time) and 5:00 PM (Pacific Time) Monday
through Friday, excluding holidays, to re port a ny Error. A list of standard holidays will be provided to Customer upon request. Company
shall have the right to determine in its sole discretion what corrective action Company will perform to support the Licensed Software.
Company may subcontract the Services to a third party contractor provided that Company will be responsible for the third party
contractor's compliance with this Agreement.
3.3 Service Exclusions. Company will not be obligated to provide the Services if (a) Company determines that an Error is caused by
malfunction of any hardware (other than malfunction of the System) or third party software used with the Licensed Software; or (b)
Customer has failed to incorporate the latest Update previously released to Customer.
4. Warr anty
4.1 Licensed Software Warranty. Company warrants that the Licensed Software, as properly installed, and under normal use, will
perform substantially in accordance with its Documentation during the Warranty Period. The “Warranty Period” for the Licensed
Software begins on date Customer downloads the Licensed Software and ends twelve (12) months thereafter.
4.2 Remedy. If Customer notifies Company in writing during the Warranty Period of an Error, Company will, at its expense and as its sole
obligation for any breach of the foregoing warranty, use commercially reasonable efforts to correct the Error or replace the Licensed
Software. Any Error correction or replacement of the Licensed Software will not extend the original Warranty Period. The warranty and
the remedies provided above will not apply to the Licensed Software if (a) Company determines that an Error is caused by accident,
abuse, misuse, negligence, fire, earthquake, flood, other force majeure event, failure of electrical power, the use of unauthorized
products or unauthorized repairs or modifications; (b) Company determines that an Error is caused during or as a result of delivery; (c)
a problem arises from or is based on Company's compliance with Customer's specifications; or (d) Company determines that an Error
is caused by malfunction of any hardware (other than malfunction of the System) or third party software used with the Licensed
Software.
4.3 Disclaimer. THE WARRANTIES ABOVE ARE EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER EXPRESS, IMPLIED OR
STATUTORY, INCLUDING WITHOUT LIMITATION THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR
PURPOSE, TITLE AND NONINFRINGEMENT.
5. Limitation of Liability. NEITHER COMPANY NOR ITS SUPPLIERS SHALL BE RESPONSIBLE OR LIABLE WITH RESPECT TO ANY SUBJECT MATTER
OF THIS AGREEMENT OR TERMS OR CONDITIONS RELATED THERETO UNDER ANY CONTRACT, NEGLIGENCE, STRICT LIABILITY OR OTHER
THEORY (A) FOR LOSS OR INACCURACY OF DATA, LOSS OF PROFITS OR COST OF PROCUREMENT OF SUBSTITUTE GOODS, SERVICES OR
TECHNOLOGY, OR (B) FOR ANY INDIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES INCLUDING, BUT NOT LIMITED TO LOSS OF
REVENUES AND LOSS OF PROFITS. COMPANY'S AGGREGATE CUMULATIVE LIABILITY HEREUNDER SHALL NOT EXCEED THE GREATER OF FIVE
HUNDRED DOLLARS ($500.00).
6. Term and Termination
6.1 Term of Agreement. The Agreement is effective on the date Customer downloads the Licensed Software and shall remain in effect
until terminated by either party as provided in this section.
6.2 Termination For Material Breach. Either party may terminate this Agreement upon written notice if the other party materially
breaches this Agreement and fails to cure such breach within thirty (30) calendar days following receipt of written notice from the
other party specifying the breach in detail. Notwithstanding the foregoing, Company may immediately terminate this Agreement and
all licenses granted hereunder if Customer breaches Section 2 (License and Restrictions) hereof or upon termination of the System
Quotation. The foregoing rights of termination are in addition to any other rights and remedies provided in this Agreement or by law.
6.3 Effect of Termination. Upon termination of this Agreement (or termination or expiration of any license granted hereunder), all rights
of Customer to use the Licensed Software and Documentation will cease and (a) all license rights granted under this Agreement will
immediately terminate and Customer shall promptly stop all use of the Licensed Software and Documentation; (b) all Services will
terminate immediately; (c) Customer shall promptly erase all copies of the Licensed Software from Customer's computers, and destroy
all copies of the Licensed Software and Documentation on tangible media in Customer's possession or control or return such copies
to Company; and (d) upon request by Company, Customer shall certify in writing to Company that it has returned or destroyed such
Licensed Software and Documentation. The parties' rights and obligations under Sections 1 (Definitions), 2.4 (Ownership), 4.3
(Disclaimer), 5 (Limitation of Liability), 6 (Term and Termination), and 7 (General) shall survive termination of this Agreement.
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7. General
7.1 Assignment. This Agreement and Customer's rights hereunder may not be assigned to any third party by Customer except with the
prior written approval of Company. Any attempted assignment of this Agreement or any rights or obligations hereunder will be null
and void.
7.2 Governing Law. This Agreement is made in, governed by, and shall be construed in accordance with the laws of the State of
California, without regard to any conflicts of law principles that would result in application of laws of any other jurisdiction. The United
Nations Convention on Contracts for the International Sale of Goods does not apply to this contract. Any legal action or other legal
proceeding relating to this contract or the enforcement of any provision of this contract must be brought in any state or federal court
located in Santa Clara County, California. Customer and Company expressly and irrevocably consents and submits to the jurisdiction of
such courts.
7.3 Injunctive Relief. Customer acknowledges that the Licensed Software contains valuable trade secrets and proprietary information of
Company, that any actual or threatened breach of this Agreement will cause harm to Company for which monetary damages would
be an inadequate remedy, and that injunctive relief is an appropriate remedy for such breach.
7.4 Modifications. Company reserves the right to change the terms and conditions of this Agreement or its policies relating to the
Licensed Software at any time. Company will notify Customer of any material changes to this Agreement by sending Customer an email to the last e-mail address Customer provided to Company or by prominently posting notice of the changes on Company's
website. Any material changes to this Agreement will be effective upon the earlier of thirty (30) calendar days following Company's
dispatch of an e-mail notice to Customer or thirty (30) calendar days following Company's posting of notice of the changes on
Company's website. These changes will be effective immediately for new users of our Licensed Software. Please note that at all times
Customer is responsible for providing Company with its most current e-mail address. In the event that the last e-mail address that
Customer has provided Company is not valid, or for any reason Company is not capable of delivering to Customer the notice
described above, Company's dispatch of the e-mail containing such notice will nonetheless constitute effective notice of the changes
described in the notice. If Customer does not agree with the changes to this Agreement, Customer must notify Company prior to the
effective date of the changes that Customer wishes to terminate its license to the Licensed Software. Continued use of the Licensed
Sof tware, following notice of s uch cha nges, s hall in dicate Customer's ac knowledgement o f such c hanges and agreem ent to b e bound
by the terms and conditions of such changes.
7.5 Severability. In the event any provision of this Agreement is held to be invalid or unenforceable, the remaining provisions of this
Agreement will remain in full force.
7.6 Waiv er. The waiver by either party of any default or breach of this Agreement shall not constitute a waiver of any other or subsequent
default or breach.
7.7 Export. Customer agrees not to export, reexport or transfer, directly or indirectly, any U.S. technical data acquired from Company, or
any products utilizing such data, in violation of the United States export laws or regulations.
7.8 Force Majeure. Company shall not be liable, directly or indirectly, for any delay or failure in performance of any obligation under this
Agreement, including any delivery obligation, where such delay or failure arises or results from a cause beyond Company's reasonable
control, or beyond the reasonable control of Company's suppliers or contractors, including, but not limited to strike, boycott or other
labor disputes, embargo, governmental regulation, inability or delay in obtaining materials, acts of God, war, earthquake, fire or flood.
In the event of such force majeure, the time for delivery or other performance will be extended for a period equal to the duration of
the delay caused thereby, provided that Company notifies Customer of the nature and duration of such force majeure event.
7.9 Entire Agreement; Notice. This Agreement constitutes the complete agreement between the parties and supersedes all prior or
contemporaneous agreements or representations, written or oral, concerning the subject matter of this Agreement. Except as
otherwise expressly provided in this Agreement, any modifications of this Agreement must be in writing and agreed to by both
parties. Company may provide any notice to Customer by e-mail. Customer may provide notice to Company by sending an e-mail to
info@proteinsimple.com or a letter by United States mail to ProteinSimple, 3001 Orchard Parkway, San Jose, CA 95134, or to such other
address as Company may specify in writing by posting the new address on the Company website.
7.10 Relationship of the Parties. The parties are acting hereunder as independent contractors and not as partners, agents, fiduciaries or
joint venturers. Neither party has the power or authority represent, act for, bind or otherwise create or assume any obligation on behalf
of the other party.
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