PolyScience 3032 series Instructions For Use Manual

3032 MICROSCOPE SERIES

INSTRUCTIONS

US Headquarters

400 Valley Road
Warrington, PA 18976

[phone] / [phone]
[phone] / [phone] fax
[e-mail]
www.polysciences.com

Our catalog lists over 3,000 unique and specialty products that are used by histology professionals, lab technicians,
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European Headquarters

Handelsstrasse 3
D-69214 Eppelheim
Germany

(49) 6221-765767
(49) 6221-764620 fax
[e-mail]

Asia-Pacific Headquarters

Polysciences Asia Pacific, Inc.
2F-1, 207 DunHua N. Rd.
Taipei, Taiwan 10595

(886) 2 8712 0600
(886) 2 8712 2677 fax
[e-mail]

3032

SAFETY NOTES

. Ope

3

2

1

Figure 1

1

2. Do not discard the molded Styrofoam container; the container

3. Keep the instrument out of direct sunlight, high temperature or

4. If any specimen solutions or other liquids splash onto the stage,

5. CAUTION: The lamp, lamp house (Fig.1-3) and adjacent parts

the shipping carton carefully to prevent any accessory, i.e.

n

objectives or eyepieces, from dropping and being damaged.

should be retained should the microscope ever require

reshipment.

humidity or dusty environments. Ensure the microscope is

located on a smooth, level and firm surface.

objective or any other component, disconnect the power cord

immediately and wipe up the spillage. Otherwise, the instrument

may be damaged.

will become very hot. Do not touch these parts until they have

completely cooled. Never attempt to handle a hot halogen bulb.

6. All electrical connectors (power cord) should be inserted into an

4

Figure

2

electrical surge suppressor to prevent damage due to voltage

fluctuations.

7. For safety when replacing the halogen lamp or fuse, ensure the

main switch is off (“O”), remove the power cord, and replace the

halogen bulb after the bulb and the lamp house has completely

cooled.

8. Confirm that the input voltage indicated on your microscope

corresponds to your line voltage. The use of a different input

voltage than indicated will cause severe damage to the

microscope.

9. When moving the microscope, hold the instrument with one

hand on the lower portion of the eyepiece tube (Fig. 1-1) and the

other hand on the illumination bracket (Fig. 1-2)

10. Halogen lamp: 6 volt 30 watts. Do not use a lamp with

different specifications.

CARE A

. Do no

1

2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on metal

3. The outer surface of the optics should be inspected and cleaned periodically using an air

t attempt to disassemble any component including eyepieces, objectives or focusing

assembly.

surfaces should be cleaned with a damp cloth. More persistent dirt should be removed using

a mild soap solution. Do not use organic solvents for cleansing.

stream from an air bulb. If dirt remains on the optical surface, use a soft cloth or cotton

swab dampened with a lens cleaning solution (available at camera stores). All optical lenses

should be swabbed using a circular motion. A small amount of absorbent cotton wound on

the end of a tapered stick makes a useful tool for cleaning recessed optical surfaces. Avoid

using an excessive amount of solvents as this may cause problems with optical coatings or

cemented optics or the flowing solvent may pick up grease making cleaning more difficult.

Oil immersion objectives should be cleaned immediately after use by removing the oil with

lens tissue or a clean, soft cloth.

ND MAINTENANCE

4. Store the instrument in a cool, dry environment. Cover the microscope with the dust cover

when not in use.

5. Microscopes are precision instruments which require periodic servicing to maintain

prope

r performance and to compensate for normal wear. A regular schedule of

preventative maintenance by qualified personnel is highly recommended. Your authorized

distributor can arrange for this service.

SAFETY SYMBOLS

Symbol Meaning

The su

Before

rface is very hot. Do not touch with your hands.

using, please read the instructions carefully. Improper operation may

result in injury or microscope malfunction.

The ma

The ma

in switch is “on”

in switch is “off”

Phase Annuli Slider

Trinocular Viewing

Head

Lamp House

Illum

Bracket

ination

Eyepieces

Stage

Variable Light Intensity

Adjus

tment Dial

Base

Conden

Fine Focus Knob

ser

X & Y

Stage

ovement

M

Controls

Coarse Focus

Knob

3032 INVERTED MICROSCOPE SERIES

On/Off Switch

1

2．．．．Assembly

2.1 Assembly Diagram

The following figure shows the correct installation seque

components in the exact numerical sequence as in the diagram.

 Inspect all optical surfaces for dust and debris; clean if necessary.
 Please save the hexagonal wrench for future use.
 Retain all stryofoam cartons if reshipment is ever required.

nce of the components. Assemble the

3032

Filter Holder ((10)

Eyepieces (8)

Color Filter (9)

Lamp House (3)

Condenser Illumination

bly (1)

Assem

BNC Electrical Cable (2)

Glass Stage Plate (7)

Objectives (4)

Power Cord (11)

Microscope Base

Stage Extension (6)

Mechanical Stage (5)

Figure 2

2

1

3

5

6

4

2

3032

2.2 Assembly Steps

2-2-1 Installing and Replacing the Lamp (Figure 3）



se the specified halogen lamp: 6V30W

 U





4

Wrap the bulb (1) with gauze or lint free paper; press the

pins (2) into the socket (3) in the lamp house.

Figure 3

When replacing the lamp turn the main switch to “O” (off)

and remove the power cord. Allow the lamp, lamp house

and the adjacent areas to sufficiently cool before handling.

The lamp will become very hot and will cause burns.

 Do n

Fingerprints on the bulb may shorten the bulb life or

interfere with the illumination. Clean all fingerprints

with a dry soft cloth.

ot touch the halogen bulb with your hands.

2-2-2 Installing the Condenser Illumination
Assem

bly（（（（Figure 4））））

Figure 4

4

3

2

1

1. Insert the condenser illumination unit (1) into the

bracket (2).

2. Turn the condenser illumination unit at clockwise
about 90 until the “AS” mark of filter holder③ is

facing forward.

3. Keep the screws the of condenser illumination

unit and the holes of the bracket aligned. Tighten the

screws with the supplied hexagon wrench.

4. Insert BNC connector cable ④ into the BNC
connector plug ⑤.

2-2-3 Installing the Lamp House (Figure 5)

the BNC connector plug① and the lamp house

Keep
pin② aligned. Also keep the bolt③ and the condenser
jack④ aligned. Then gently push the lamp house into

the illumination unit until they are completely

connected.

Figure 5

）

）

）

3

1

2

Figure 6

3

Figure 7

1

2-2-4 Installing the Objectives（

1. Turn the coarse focusing knob① clockwise until the

nosepiece reaches its lowest position. The tension

control collar (2) has been factory adjusted.

2. Install the lowest magnification objective into the

nosepiece. Then, in a clock-wise direction, rotate the

nosepiece and install each succeeding higher

magnification objective.

3. When changing objective magnifications, rotate the

nosepiece until you hear a “click” sound. This ensures

the objective is centered in the optical light path.

★ Inspect the objectives regularly for dirt and oil; clean

necessary.

★ Cover all unused nosepiece holes with a nosepiece

plug③ to prevent dust and contamination from entering.

★ Use the 10x objective to initially focus the image of your

imen.

spec

2-2-5

Extension

1. Attach the mechanical stage to the right side of the fixed

2. Attach the stage extension to the left side of the fixed

Attaching the Mechanical Stage and Stage

（（（（

Figure 8

stage by tightening the two thumb screws on the

underside of the mechanical stage.

stage by tightening the two thumb screws (1) on the

underside of the stage extension.

Figure 8

4

3032

））））

（Fig

（

（

6 & 7））））

.

if

1

2-2-6 Installing the Stage Plate（

1. Install the glass stage plate ① into the stage opening.

Figure 9

1

2-2-7 Installing the Eyepieces（（（（Figu

1. Remove the protective caps on the eyepiece tubes①.

3032

（Fig

（

（

re 9））））

u

re 10））））

2

Figure 10

2. I

nsert completely the eyepieces into eyetubes.

2-2-8 Installing the Color Filters (Figure 11)

★ Allow the color filter to cool completely before you

e them. Remove the filter holder①, and then

chang

insert the color filter ②required.

2

● Mount the color filter so it lays flat ③ in the holder as

shown.

★ The color filter must be laying flat. If not, the filter

1

3

Figure 11

has b

een installed incorrectly④④④④.

● More than one filter may be installed only if the total

thickness is less than 11mm.

5

3032

2-2-9 Connecting the Power Cord（

1

Figure 12

and 14））））

★

★ Do not place stress or strain on the power

★ ★

cord. These will damage the cord and cause
a danger to the user.

1. Turn the main switch ① to “O” (off) before

connecting the power cord.

2. Insert the power cord plug ② into the electrical
connector ③.

3. Insert the power cord ④ into an electrical

receptacle (5).

4. Insert the BNC connector plug ⑨ into the BNC
connector jack ⑧.

★★★★ Ensure the power cord is connected to a

grounded receptacle.

（Fig

（

（

12, 13

.

Use of an electrical surge suppressor receptacle

is highly recommended.

Figure 13

2-2-10 Replacing the Fuse (Figures 12 & 13））））

urn the main switch ① to “O”

T

replacing the fuse. Unplug the power cord.

(off) before

Rotate the fuse holder ⑥ out of the base ⑦

5

4

with a flat edge screwdriver, replace the fuse,

then insert the fuse holder and tighten.

★Fuse rating: 250V，，，，500mA.

Figure 14

6

3. Adjustment 3032

Diopter ring

Light path selector lever

Light switch

Tension adjustment collar

Aperture diaphragm adjust lever

Phase center bolt

Main switch Coarse focus knob Fine focus knob

Figure 15

Color filter holder

Phase contrast slider

7

4. Adjustments 3032

4-1 M

icroscope base

Figure 16

Figure 17

1

1

3

2

1

Figure 18

4-1-1 Turning on the Lamp（

（Fig

（

（

Connect the power cord; turn the main switch ①
to “－” (on).

re 16））））

u

4-1-2 Adjusting the Brightness（（（（Figu

Rotate the variable light intensity dial (Fig. 17-1)

clockwise to increase the brightness. Rotating the dial

counterclockwise reduces the brightness.

Using the lowest feasible brightness will increase

the bulb life.

re 17））））

4-1-3 Adjusting the Tension Adjustment

r（（（（Figure 18））））

Colla

★

The tension of the coarse focus knob

factory adjusted.

Rotate the tension adjustment collar ① in the

direction shown by the arrow (Figure 18) to increase
the tension of the coarse focus knob ②. Rotating the

collar in the opposite direction will decrease the

tension.

The coarse focus knob is too loose if the nosepiece

drops automatically or if the specimen looses focus
soon after focusing with the fine focus knob ③.

Tighten the tension adjustment collar if either of

these occurs.

②②②②

has been

8

3032

4-2 S

tage

3

2

Figure 19

5

Figure 20

1

6

7

4

4-2-1 Viewing the Specimen（

For optimum viewing, ensure the thickness, as

marked on each objective (0.17mm or 1.2mm) is the

same as your container, dish or slide.

Use the appropriate bracket to hold your Terasaki well

plate (3), culture dish (4), flask (5) or slide (5). A larger

flask may be placed directly on the stage. Slides may

also be placed by securing with the slide holder (2).

The specimen is positioned by turning the X (6) and Y (7)

stage movement controls.

（Fig

（

（

res 19 & 20））））

u

9

3032

4-3 V

iewing Adjustments

D

Figure 21

Figure 22

2

3

4-3-1

1. Using the 10x objective and your right eye

2. Then observe the specimen with your left eye

The diopter range is ±5

4-3-2 Interpupillary Distance Adjustment

iopter Adjustment（

only, observe your specimen through the right

eyepiece and bring it into focus,

only through the left eyepiece. If the specimen

is not in focus, rotate the diopter ring① until a

sharp image is obtained.

（（（（Figu

While observing with both eyes, hold the

left and right prism eye tubes. Rotate the

eye tubes around the central axis. The

interpupillary distance is correct when the

left and right fields of view coincide
completely with one image.

The interpupillary distance range is

48-75mm.

（F

（

（

re 22））））

gure 21））））

i

10

S

Figure 23

1

4-3-3

★ The light path desired is obtained by pulling out or

● For binocular observation, push the light path selector lever

● For trinocular observation, pull the light path selector lever

Selecto

witching the Light Path（

pushing in the light path selector lever ①.

“in” completely.

“out” completely.

Light Path

r Lever

Illumination

Proportions

（F

g.23））））

i

（

（

Applications

Pushing in the

lever

ompletely

c

Pulling out the

lever

ompletely

c

Epi Fluorescent

Models

100% for

Binocular

observation

20% for

Binocular

observation

and 80% for

Trinocular

observation

100%

Binocular

or

100%

Trinocular

Binocular

observation

Binocular

observation,

video monitor,

computer monitor

and

microphotography

Fluorescence

Applications

11

3032

4-4 I

llumination Unit

1

2

Figure 24

70-80%

30-20%

Figure 25

U

4-4-1

Select the appropriate color filter according to the

sing Color Filters（

type of illumination being used. Insert the filter

（F

gure 24））））

i

（

（

into the filter holder.

The filter holder can hold multiple filters up to a

total thickness of 11mm or less.

Color

Filter

IF550

LBD

Us

age in Microscopy

Filter

Green

use for phase contrast microscopy

ilter

Blue F

use for bright field observation and

microphotography

4-4-2 Using the Aperture Diaphragm（（（（Figu

● When using bright field observation, the aperture diaphragm is used to control the numerical

re 25））））

aperture of the illumination system not the brightness. Only when the numerical apertures of the

objective and the illumination system are equal can higher image resolution, contrast, and

increased depth of field be obtained.

● Generally, when observing the chromatic specimens, the N.A. of the condenser aperture diaphragm

is adjusted to 70-80% of the numerical aperture marked on the objective.

To adjust the aperture diaphragm, remove the eyepiece then looked into the viewing tube. Your field of

view will appear as Figure 25. The proportion may be changed by turning the aperture adjustment
lever: (①is the image of the aperture diaphragm & ② is the edge of the objective).

12

5．

hase Contrast Microscopy 3032

．P

．

．

5-1

Nomenclature

Figure 26

1 2

Figure 27

5-1-1

Phase CCCContrast

Phase

Pha

ontrast OOOObjective

se Phase

ontrast ontrast

bjective（（（（FFFFig

bjectivebjective

The microscope’s standard phase objectives are

10x and 20x. They are mounted on the objective

turret as described in 2-2-4.

5-1-2 Phase Contrast Annulus Slider

(Figure 27)

● The phase annulus ring has been centered. The phase

annul

us ring may be adjusted by using the centering

telescope as described in 5-2-2.

● The 10X/20X phase ring ① is used with the 10X and

20X phase contrast objectives. The empty opening

② is used for bright field observation.

igure

ure 26

igig

ure ure

26））））

2626

13

Phase Contrast Microscopy 3032

5-2 I

nstallation and use

2

1

Figure 28

1

2

Figure 29

3

Figure 30

5-2-1 Installing the Phase Contrast Slider（

（Fig

（

（

28））））

1. With the slider ① facing upwards (the surface with

printing), insert it into the illumination system.

2. Brightfield and phase contrast light rings have their

own position. Move the slider until you hear the

“click” to ensure the ring or the opening is centered

in the light path.

3. When observing with a phase contrast objective,

keep the aperture diaphragm adjustment lever ② in

the position “O” (open).

5-2-2Centering Telescope (Figures 29 and 30））））

phase annulus has been pre-centered.

The

Adjustments will usually not be required with

normal usage.

If adjustments are required:

1. Place the specimen on the stage and focus as usual.

2.

Remove the eyepiece without the diopter adjustment

and replace it with the centering telescope.

3. Ensure sure the matched phase contrast objective and

annulus ring in the phase contrast slider are in the

center of the light path.

4. Using the centering telescope to observe the light

ring’s image ① and the phase contrast ring’s
image②. If the light ring’s image is not sharp, turn

the recessed screws in the phase annulus slider until
you can see a clear image of the light ring②

superimposed on light ring (1). The best image will

be obtained only when the two rings coincide.

5. Changing containers, dishes or slides of different

thicknesses will require re-adjustment of the phase

annulus.

u

re

14

6．．．．Microscope Video and Photography 3032

6-1

Microscope Video

6-1-1 Selecting the Light Path（

1

Figure 31

Video

CCD

1. For binocular observation, push the light path selector

lever (1) in completely.

2. Focus your specimen as usual.

3. For video or camera usage, pull horizontally on the light

path selector lever until it is completely pulled out.

6-1-2 Installing the Video Adapter（（（（Figu

1. Loosen the locking thumb screw ① on the trinocular

viewing head and remove the protective cap②.

2. Remove the protective caps on the both ends of the

video adapter③.

3. Screw the threaded end into the CCD/CMOS port and

tighten the thumb screw on the adapter (3).

3. Install the accessories into the vertical port and tighten

the thumb screw ①.

4. Attach your video camera to the video port (4).

6-1-3 Focusing（（（（Figu

re 32））））

（Fig

（

（

u

re 31））））

re 32））））

2

Figure 32

4

3

1

Focus on your specimen while observing through the

eyepieces. Ensure the light path selector lever is fully

pulled out. Observe the image on the video or

computer monitor. If the image is not in focus, turn the

revolving video tube④ until the image is sharp.

14

3032

6-2 M

icroscope Photography

2

Figure 33

Camera

4

3

1

6-2-1 Selecting the Light Path (Figure 31)

1. Fo

r binocular observation, push in completely the

light path selector lever (1).

2. Focus your specimen as usual.

3. For microphotography, pull horizontally on the light

path selector lever until it is completely pulled out.

6-2-2 Installing the Photo Camera（

（Fig

（

（

1. Loosen the locking thumb screw① on the

trinocular viewing head and remove the
protective cap②.

2. Install the adapter③ into the vertical port, and

tighten the thumb screw ①.

3. Insert the camera adapter into ④ into the camera

port.

● Camera magnification = objective magnification x

camera

★ The shutter on some cameras may cause a jarring

lens magnification

impact when photographing through the

microscope. To weaken the impact and obtain a

clear image, select a longer exposure time.

6-2-3 Focusing

cus as usual in the binocular mode. When using the

Fo

microscope for photography, use the camera’s viewfinder

to focus the specimen. Please refer to the camera’s user

manual for additional details.

15

re 33））））

u

7.Specifications 3032

Optical

Sy

stem

Viewing

Head

Eyepiece Wide Field Eyepiece 10X; 22 mm field of view

Nosepiece Reversed Quintuple Nosepiece

Objectives

Focusing

m

Syste

Mechanical

Stage

Illumination Halogen Lamp 6volt 30watt; Variable intensity

Condenser Long working Distance Condenser, N.A. 0.3; Working Distance 72mm

tive Specifications

Objec

TYPE

Infinity

Long

orking

W

Distance

Plan

Achromatic

Objective

Infinity

Long

orking

W

Distance

Plan Phase

Contrast

Objective

Infinity Optical System

C

ompensation Free Trinocular Head Inclined 30º

Division ratio: 20% for Binocular Viewing and 80% for Video Imaging &

Microphotography

Infin

ity Long Working Distance Plan Achromatic: 4X & 40X

Infinity Long Working Distance Plan Phase Contrast: 10X & 20X

Coaxial Coarse and Fine Focusing System

Sensitivity and Graduation of Fine Focus: 0.002mm

Movement Range(from the surface focus of stage plate): up 8mm, down 3mm

Size: 250mm x 160mm
Movement Range: 120mm（width）×78mm（length）

MAGNIFICATION

4X 0.1 25.2 ∞ 45 ─

40X 0.6 3

10X 0.25 11 ∞ 45 0.17mm

20X 0.4 6 ∞ 4

NUMERICAL

APERTU

（N.A）

RE

WORKING

DISTANCE

（mm）

.2 ∞ 45 1.2mm

CONJUGATE

DISTANCE

（mm）

FOCUS

DISTANCE

（mm）

5 0.17mm

THICKNESS

COVER

SLIP

16

TROUBLESHOOTING GUIDE

If a

problem occurs during the course of use, please refer to the tables below

before contacting your distributor.

OPTICAL

Problem Cause

Darkness at the periphery Revolving nosepiece not in
or uneven brightness in the click stop position swingi
field of view

Dirt or dust on the viewfield Dirt or dust on the lens - Clean the lens
eyepiece
objecti
specime

Poor image quality No cove
the sl

Covergl

Slide m
glass

Immersio
objecti

No imme
100xR

Air bub

Conden
or open

Conden
low the

Image moves while focusing Specimen rises from stage Secure the specimen in the
surface s

Revolv
in the

Image tinged yellow Blue fi

co

, condenser,

ve, collector lens or

n

rglass attached to Attach a 0.17mm coverglass

ide

ass is too thick or thin Use a coverglass of the

ay be upside down Turn slide over so the cover-

n oil is on a dry Check the objectives, clean

ve (especially the 40xR) if necessary

rsion oil used with Use immersion oil

objective

bles in immersion oil Remove bubbles

ser aperture is closed Open or close properly

too much

ser is positioned too Position the condenser at

IMAGE PROBLEMS

ing nosepiece is not Revolve the nosepiece to the

click-stop position click-stop position

lter not used Use daylight blue filter

C

orrective Measure

v

Revol
click-stop position by

approp
(0.17mm)

e the nosepiece to

g the objective

n

y

rrectl

lide holder

into the optical path

riate thickness

faces up

upper limit

IMAGE PROBLEMS

Problem Cause

Image tinged yellow Lamp

Image is too bright Lamp

Insufficient brightness Lamp intensity is too low Adjust the light intensity by
rotat
dial

Aperture
too f

Conde
the

Image will not focus with Slide upside down Turn t
high power objectives cover

Cover

High power objective contacts Slide upside down Turn t
slide when changed from cover
low power objective
Cover

Diopt
properl

Lamp does not light when No electrical power Check p
switched on
Lamp

Fuse

Slippage of focus when using Tension adjustment is set too Increase the tension on the
the coarse focusing knob low focusi

Fine focus is ineffective Tension adjustment is set too Loosen the tension on the
high f

intensity is too low Adjust the light intensity by
rota
dia

intensity is too high Adjust the light intensity by
rota
dia

diaphragm closed Open to the proper setting

ar

nser position too low Position the condenser at

MECHANICAL PROBLEMS

glass is to thick Use a 0.17mm cover glass

glass is to thick Use a 0.17mm cover glass

er adjustment is not set Readjust the diopter settings

y

bulb burnt out Replace bulb

blown out Replace fuse

Corrective Measure

ing the intensity control

t

l

ing the intensity control

t

l

ing the intensity control

upper limit

he slide over so the

glass faces up

he slide over so the

glass faces up

ower cord connection

ng knobs

ocusing knobs

Order online anytime at www.polysciences.com

US Headquarters

400 Valley Road
Warrington, PA 18976

European Headquarters

Handelsstrasse 3
D-69214 Eppelheim
Germany

Asia-Pacific Headquarters

Polysciences Asia Pacific, Inc.
2F-1, 207 DunHua N. Rd.
Taipei, Taiwan 10595

[phone] / [phone]
[phone] / [phone] fax
[e-mail]
www.polysciences.com

(49) 6221-765767
(49) 6221-764620 fax
[e-mail]

(886) 2 8712 0600
(886) 2 8712 2677 fax
[e-mail]

Our catalog lists over 3,000 unique and specialty products that are used by histology professionals, lab technicians,
pathologists, chemists and scientists worldwide. Visit www.polysciences.com to learn more about our product lines.