PASCO ME-6936 User Manual

Metabolism Chamber

Carbon Dioxide Gas Sensor

(not included)

Instruction Manual

012-10844A

ME-6936

Lid

Oxygen Gas Sensor

(not included)

Cable not to scale

Hole

Introduction

PASCO model ME-6936 Metabolism Chamber is a 250-mL Nalgene© sampling bottle designed to work with a PASCO Oxygen Gas Sensor and a Carbon Dioxide Gas Sensor for simultaneous measurement of oxygen gas and carbon dioxide gas during the study of metabolism.

Sensor Model

PASPORT Oxygen Gas Sensor PS-2126

PASPORT Carbon Dioxide Gas Sensor PS-2110

Oxygen Gas Sensor CI-6562

Carbon Dioxide Gas Sensor CI-6561

Recommended Model

Fast Response Temperature Probe PS-2135

PASPORT Sensor Extension Cable PS-2500

One of the most popular methods of exploring cellular respiration in the biology lab is to measure the production of carbon dioxide and the consumption of oxygen by germinating seeds.

The PASPORT gas sensors are designed to work with a PASPORT interface such as the ones listed below.

PASPORT Interface Model

Xplorer GLX PS-2002

Xplorer PS-2000

PowerLink PS-2001

USB Link PS-2100

SPARK Science Learning System PS-2008

The other gas sensors work with a ScienceWorkshop interface.

ScienceWorkshop Interface Model

ScienceWorkshop 750 CI-7650

ScienceWorkshop 500 CI-6400

See the PASCO catalog or the PASCO web site at

www.pasco.com

for more information about PASCO sensors and interfaces.

800-772-8700 www.pasco.com

Model No. ME-6936 Sample Activities

Sample Activities

1) Introductory Cell Respiration Lab

Teacher Preparation

• Get Alaska pea seeds (or lima beans) - about 100 per lab group. Get glass beads approximately the same size as the pea seeds (about 25 per lab group).

• Three or four days before the lab, soak the pea seeds in a pan of water overnight. Soak approximately 25 seeds per lab group.

• Twenty-four hours later, remove the seeds from the water and place them on a moist paper towel.

• Place the seeds and towel into a plastic bag or plastic, lidded storage container and leave in a warm, dry place for twenty-four hours. NOTE: Do not seal the bag or the plastic storage container.

• Provide each lab group with 25 glass beads, 25 dry seeds, and 25 germinating seeds.

Data Collection

NOTE: Do not seal the plastic bag or plastic storage container.

1. Start a new experiment on the data collection system.

2. Connect a carbon dioxide (CO

Use the sensor extension cable to connect the CO

3. Calibrate the CO

sensor and calibrate the O2 sensor.

4. Change the unit of measurement for the O

5. Set up two graphs to display simultaneously. One one graph, display CO

and Time on the x-axis. On the other graph, display O

) gas sensor and an oxygen (O2) gas sensor to the data collection system.

sensor to the collection system.

sensor from “%” to “ppm”.

concentration on the y-axis and Time on the

concentration on the y-axis

x-axis.

6. For the first trial, place 25 glass beads into the Metabolism Chamber.

7. Start recording data.

8. Insert the CO

sensor into the top of the Metabolism Chamber and the O2 sensor into the hole on the

side of the Metabolism Chamber. Firmly press the rubber stoppers of the sensors into the chamber. The Metabolism Chamber must remain vertical; do not lay it on its side.

9. Adjust the scale of the graphs to show all data.

10. After 10 minutes, stop recording data.

11. Name the data run.

12. Remove the sensors and the glass beads from the Metabolism Chamber. Wave the chamber so that the

air in the chamber mixes with air in the room.

13. For the second trial, place 25 dry seeds into the Metabolism Chamber.

14. Start recording data. Replace the sensors into the Metabolism Chamber. Remember that the chamber

must remain vertical.

15. Adjust the scale of the graphs to show all data.

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