®
Metabolism Chamber
Carbon Dioxide Gas Sensor
(not included)
Instruction Manual
012-10844A
ME-6936
Lid
Oxygen Gas Sensor
(not included)
Cable not to scale
Hole
Introduction
PASCO model ME-6936 Metabolism Chamber is a 250-mL Nalgene© sampling bottle designed to work with a PASCO Oxygen
Gas Sensor and a Carbon Dioxide Gas Sensor for simultaneous
measurement of oxygen gas and carbon dioxide gas during the
study of metabolism.
Sensor Model
PASPORT Oxygen Gas Sensor PS-2126
PASPORT Carbon Dioxide Gas Sensor PS-2110
Oxygen Gas Sensor CI-6562
Carbon Dioxide Gas Sensor CI-6561
Recommended Model
Fast Response Temperature Probe PS-2135
PASPORT Sensor Extension Cable PS-2500
One of the most popular methods of exploring cellular respiration
in the biology lab is to measure the production of carbon dioxide
and the consumption of oxygen by germinating seeds.
The PASPORT gas sensors are designed to work with a
PASPORT interface such as the ones listed below.
PASPORT Interface Model
Xplorer GLX PS-2002
Xplorer PS-2000
PowerLink PS-2001
USB Link PS-2100
SPARK Science Learning System PS-2008
The other gas sensors work with a ScienceWorkshop interface.
ScienceWorkshop Interface Model
ScienceWorkshop 750 CI-7650
ScienceWorkshop 500 CI-6400
See the PASCO catalog or the PASCO web site at
www.pasco.com
for more information about PASCO sensors and interfaces.
800-772-8700 www.pasco.com
Model No. ME-6936 Sample Activities
Sample Activities
1) Introductory Cell Respiration Lab
Teacher Preparation
• Get Alaska pea seeds (or lima beans) - about 100 per lab group. Get glass beads approximately the
same size as the pea seeds (about 25 per lab group).
• Three or four days before the lab, soak the pea seeds in a pan of water overnight. Soak approximately
25 seeds per lab group.
• Twenty-four hours later, remove the seeds from the water and place them on a moist paper towel.
• Place the seeds and towel into a plastic bag or plastic, lidded storage container and leave in a warm,
dry place for twenty-four hours. NOTE: Do not seal the bag or the plastic storage container.
• Provide each lab group with 25 glass beads, 25 dry seeds, and 25 germinating seeds.
Data Collection
NOTE: Do not seal the
plastic bag or plastic
storage container.
1. Start a new experiment on the data collection system.
2. Connect a carbon dioxide (CO
Use the sensor extension cable to connect the CO
3. Calibrate the CO
sensor and calibrate the O2 sensor.
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4. Change the unit of measurement for the O
5. Set up two graphs to display simultaneously. One one graph, display CO
and Time on the x-axis. On the other graph, display O
) gas sensor and an oxygen (O2) gas sensor to the data collection system.
2
2
sensor to the collection system.
2
sensor from “%” to “ppm”.
concentration on the y-axis and Time on the
2
concentration on the y-axis
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x-axis.
6. For the first trial, place 25 glass beads into the Metabolism Chamber.
7. Start recording data.
8. Insert the CO
sensor into the top of the Metabolism Chamber and the O2 sensor into the hole on the
2
side of the Metabolism Chamber. Firmly press the rubber stoppers of the sensors into the chamber. The
Metabolism Chamber must remain vertical; do not lay it on its side.
9. Adjust the scale of the graphs to show all data.
10. After 10 minutes, stop recording data.
11. Name the data run.
12. Remove the sensors and the glass beads from the Metabolism Chamber. Wave the chamber so that the
air in the chamber mixes with air in the room.
13. For the second trial, place 25 dry seeds into the Metabolism Chamber.
14. Start recording data. Replace the sensors into the Metabolism Chamber. Remember that the chamber
must remain vertical.
15. Adjust the scale of the graphs to show all data.
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Model No. ME-6936 Sample Activities
16. After 10 minutes, stop recording data.
17. Name the data run.
18. Remove the sensors and the dry seeds from the chamber.
19. Wave the chamber so that the air in the chamber mixes with the air in the room.
20. For the third trial, place 25 germinating seeds into the Metabolism Chamber.
21. Start data recording. Replace the sensors into the Metabolism Chamber.
22. Adjust the scale of the graphs to show all data.
23. After 10 minutes, stop recording data.
24. Name the data run.
Data Analysis
25. Apply a linear fit to the data runs to find the slope of the line.
26. Compare the rates of CO
27. Compare the rates of O
28. Compare the rates of CO
production in the dry seeds versus the germinating seeds.
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consumption in the dry seeds versus the germinating seeds.
2
production and O2 consumption for the germinating seeds.
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2) AP© Biology Lab 5: Cellular Respiration
Teacher Preparation:
• Get Alaska pea seeds (about 100 per lab group).Get glass beads approximately the same size as the
pea seeds (about 25 per lab group).
• Three to four days before the lab, soak the pea seeds in a pan of water overnight. Soak approximately
75 pea seeds per lab group.
• Twenty-four hours later, remove the pea seeds from the water and place them on a moist paper towel.
• Place the pea seeds and the towel in a plastic bag or plastic, lidded storage container and leave it in a
warm, dry place for another 24 hours. Do not seal the bag or plastic storage container.
• On the day of the lab, prepare boiled germinating pea seeds and chilled germinating pea seeds (about
25 each per lab group).
• For each lab group, put 25 germinating pea seeds into boiling water for 5 minutes. After removing
them from the water, allow them to cool, pat them dry, and place them in a beaker labeled “boiled germinating pea seeds”.
• For each lab group, put 25 germinating pea seeds into a beaker labeled “chilled germinating pea
seeds”. Place the beaker in an ice water bath. Keep the beakers in a refrigerator until ready to use.
• For each lab group, put 25 germinating pea seeds into a beaker labeled “germinating pea seeds”.
• Provide each lab group with 25 dry pea seeds, 25 glass beads, 25 germinating pea seeds, 25 boiled
germinating pea seeds, and 25 chilled germinating pea seeds.
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Model No. ME-6936 Sample Activities
Data Collection
1. Start a new experiment on the data collection system.
2. Connect a carbon dioxide (CO
Use the sensor extension cable to connect the CO
3. Calibrate the CO
sensor and calibrate the O2 sensor.
2
4. Change the unit of measurement for the O
5. Set up two graphs to display simultaneously. One one graph, display CO
and Time on the x-axis. On the other graph, display O
) gas sensor and an oxygen (O2) gas sensor to the data collection system.
2
2
sensor to the collection system.
2
sensor from “%” to “ppm”.
concentration on the y-axis and Time on the
2
concentration on the y-axis
2
x-axis.
6. For the first trial, place 25 glass beads into the Metabolism Chamber.
7. Start recording data.
8. Insert the CO
sensor into the top of the Metabolism Chamber and the O2 sensor into the hole on the
2
side of the Metabolism Chamber. Firmly press the rubber stoppers of the sensors into the chamber. The
Metabolism Chamber must remain vertical; do not lay it on its side.
9. Adjust the scale of the graphs to show all data.
10. After 10 minutes, stop recording data.
11. Name the data run “Glass Beads”.
12. Remove the sensors and the glass beads from the Metabolism Chamber. Wave the chamber so that the
air in the chamber mixes with air in the room.
13. Repeat the data recording procedure (steps 7 to 12) for dry pea seeds, germinating pea seeds, boiled germinating pea seeds, and chilled germinating pea seeds.
Data Analysis
14. Apply a linear fit to each of the five CO
15. Apply a linear fit to each of the five O
16. Compare and analyze the rate of CO
data runs to find the slope of each line.
2
data runs to find the slope of each line.
2
production and O2 consumption in the following:
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• glass beads versus germinating pea seeds
• germinating pea seeds versus chilled germinating pea seeds
• germinating pea seeds versus boiled germinating pea seeds
• chilled germinating pea seeds versus boiled germinating pea seeds
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Model No. ME-6936 Sample Data
Sample Data
Graph 1: Germinating pea seeds in Metabolism Chamber at room temperature - CO2 (ppm) and O2 gas
(ppm) versus Time (s).
O2 Concentration
Concentration
CO
2
©
Graph 2: Sample Data from AP
Chamber at varying temperatures - CO
Biology Lab 5: Cellular Respiration. Germinating pea seeds in Metabolism
(ppm) versus Time (s).
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Germinating, room temperature
Germinating, chilled
Glass beads
Germinating, boiled
Dry pea seeds
Graph 3: Sample Data from AP Biology Lab 5: Cellular Respiration. Germinating pea seeds in Metabolism
Chamber at varying temperatures - O
(ppm) versus Time (s).
2
Glass beads
Dry pea seeds
Germinating, chilled
Germinating, boiled
Germinating, room temperature
NOTE: See the PASCO Model PS-2876 Advanced Biology Manual for
a more detailed version of the respiration labs.
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Model No. ME-6936 Technical Support
Technical Support
For assistance with any PASCO product, contact
PAS C O a t :
Address: PASCO scientific
10101 Foothills Blvd.
Roseville, CA 95747-7100
Phone: 916-786-3800 (worldwide)
800-772-8700 (U.S.)
Fax: (916) 786-7565
Web: www.pasco.com
Email: support@pasco.com
For more information about the Metabolism Chamber and the latest revision of this Instruction Manual, visit:
www.pasco.com/go?ME-6936
Limited Warranty For a description of the product warranty, see the PASCO catalog.
Copyright The PASCO scientific 012-10844A Metabo-
lism Chamber Instruction Manual is copyrighted with all
rights reserved. Permission is granted to non-profit educational institutions for reproduction of any part of this manual, providing the reproductions are used only in their
laboratories and classrooms, and are not sold for profit.
Reproduction under any other circumstances, without the
written consent of PASCO scientific, is prohibited.
Trademarks PASCO and PASCO scientific are trademarks or registered trademarks of PASCO scientific, in the
United States and/or in other countries. All other brands,
products, or service names are or may be trademarks or
service marks of, and are used to identify, products or services of their respective owners. For more information visit
www.pasco.com/legal.
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